Chrne cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrne
Entrez Gene ID	29422
Full Name	cholinergic receptor nicotinic epsilon subunit
Synonyms	nAChR
General protein information	Preferred Names cholinergic receptor nicotinic epsilon subunit Names acetylcholine receptor subunit epsilon acetylcholine receptor epsilon cholinergic receptor, nicotinic epsilon cholinergic receptor, nicotinic, epsilon (muscle) cholinergic receptor, nicotinic, epsilon polypeptide epsilon-subunit gene for acetylcholine receptor epsilon subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	subunit of the acetylcholine receptor AChR epsilon; may act at the muscle endplate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597172.1	XP_017452661.1	acetylcholine receptor subunit epsilon isoform X2
NM_017194.1	NP_058890.1	acetylcholine receptor subunit epsilon precursor
XM_017597173.1	XP_017452662.1	acetylcholine receptor subunit epsilon isoform X3
XM_017597174.1	XP_017452663.1	acetylcholine receptor subunit epsilon isoform X4
XM_017597171.1	XP_017452660.1	acetylcholine receptor subunit epsilon isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRNE	NP_000071.1
Rattus norvegicus (Norway rat)	Chrne	NP_058890.1
Xenopus tropicalis (tropical clawed frog)	chrne	XP_002941867.2
Pan troglodytes (chimpanzee)	CHRNE	XP_523556.4
Canis lupus familiaris (dog)	CHRNE	XP_536608.3
Bos taurus (cattle)	CHRNE	NP_776697.1
	CHRNE	XP_001097094.1
Mus musculus (house mouse)	Chrne	NP_033733.1

Gene Ontology
Bibliography

Related articles in PubMed

The neuromuscular junction: selective remodeling of synaptic regulators at the nerve/muscle interface.
Witzemann V, Chevessier F, Pacifici PG, Yampolsky P
Mechanisms of development130(6-8)402-11(2013 Jun-Aug)

A novel mode-of-action mediated by the fetal muscle nicotinic acetylcholine receptor resulting in developmental toxicity in rats.
Rasoulpour RJ, Ellis-Hutchings RG, Terry C, Millar NS, Zablotny CL, Gibb A, Marshall V, Collins T, Carney EW, Billington R
Toxicological sciences : an official journal of the Society of Toxicology127(2)522-34(2012 Jun)

Mammalian nicotinic acetylcholine receptors: from structure to function.
Albuquerque EX, Pereira EF, Alkondon M, Rogers SW
Physiological reviews89(1)73-120(2009 Jan)

The acetylcholine receptor gamma-to-epsilon switch occurs in individual endplates.
Yumoto N, Wakatsuki S, Sehara-Fujisawa A
Biochemical and biophysical research communications331(4)1522-7(2005 Jun)

Aberrant development of motor axons and neuromuscular synapses in MyoD-null mice.
Wang ZZ, Washabaugh CH, Yao Y, Wang JM, Zhang L, Ontell MP, Watkins SC, Rudnicki MA, Ontell M
The Journal of neuroscience : the official journal of the Society for Neuroscience23(12)5161-9(2003 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Chrne gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrne cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03195	NM_017194.1 Latest version!	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa33294	XM_017597174.1 Latest version!	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43265	XM_017597173.1 Latest version!	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa57276	XM_017597171.1 Latest version!	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa57277	XM_017597172.1 Latest version!	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrne ( NM_017194.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058890.1 Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), mRNA.

Price & Availability↑

CloneID	ORa03195
Clone ID Related Accession (Same CDS sequence)	NM_017194.1
Accession Version	NM_017194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	acetylcholine receptor subunit epsilon precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X74836.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X74836.1, X13252.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGACAATGG CTCTGCTTGG GACCCTGCTT CTCCTGGCAC TCTTTGGCAG AAGCCAAGGC 
AAGAATGAAG AGCTGAGCCT GTATCACCAT CTCTTCGACA ATTATGACCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ATGAGAAAGA AGAGACTCTG ACCACCAGTG TCTGGATTGG AATTGAATGG 
CAAGACTATC GGCTCAACTT CAGCAAGGAC GATTTTGCAG GCGTAGAAAT CCTGCGGGTC 
CCTTCCGAAC ATGTATGGCT GCCAGAGATT GTTCTGGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACTGCAA TGTTCTGGTC TATGAGGGAG GCTCTGTGAG CTGGCTGCCC 
CCAGCTATCT ACCGCAGCAC GTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG TTCCCAGACC TACAATGCTG AAGAGGTGGA GTTAATCTTT 
GCAGTGGATG ACGATGGCAA TGCCATCAAC AAAATTGACA TCGACACCGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT TGACTACTGC CCAGGCATGA TTCGCCATTA TGAGGGAGGC 
TCCACAGAAG ACCCTGGAGA AACTGACGTC ATCTACACGC TCATCATCCG TCGAAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGTGTGCTCA TTTCTGGCTT GGTGCTACTC 
GCTTACTTCC TACCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTCCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCGCTGC TGGGCAGGTA TCTTATTTTT GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG CGAACGCCAA CGACTCACGC CACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTACTG CCGCGCCTCC TCGGCTTGAG CCCACCCCCA 
GAGGATCCCG GAGCTGCCTC ACCAGCGAGG CGTGCCTCAT CTGTGGGCAT TCTGCTTAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG ACAGAGGCAT 
CGGCATGGAA CTTGGACCGC AGCCGCCCTC TGCCAGAACC TGGGTGCTGC AGCCCCTGAA 
GTCCGCTGCT GTGTGGATGC TGTGAACTTT GTGGCTGAGA GCACAAGGGA CCAGGAAGCC 
ACTGGAGAGG AACTGTCTGA CTGGGTGCGT ATGGGGAAGG CCCTCGACAA TGTCTGTTTT 
TGGGCAGCGT TGGTGCTCTT CAGCGTCGGT TCTACGCTCA TCTTCCTTGG AGGTTACTTC 
AACCAAGTTC CTGATCTCCC CTACCCACCG TGCATCCAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058890.1
CDS	42..1526
Translation	MTMALLGTLLLLALFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIEWQDYRLNFSKDDFAGVEILRVPSEHVWLPEIVLENNIDGQFGVAYDCNVLVYEGGSVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVELIFAVDDDGNAINKIDIDTAAFTENGEWAIDYCPGMIRHYEGGSTEDPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGLSPPPEDPGAASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAAALCQNLGAAAPEVRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP

Target ORF information:

RefSeq Version	NM_017194.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017194.1

ORF Insert Sequence:

ATGACAATGG CTCTGCTTGG GACCCTGCTT CTCCTGGCAC TCTTTGGCAG AAGCCAAGGC 
AAGAATGAAG AGCTGAGCCT GTATCACCAT CTCTTCGACA ATTATGACCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ATGAGAAAGA AGAGACTCTG ACCACCAGTG TCTGGATTGG AATTGAATGG 
CAAGACTATC GGCTCAACTT CAGCAAGGAC GATTTTGCAG GCGTAGAAAT CCTGCGGGTC 
CCTTCCGAAC ATGTATGGCT GCCAGAGATT GTTCTGGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACTGCAA TGTTCTGGTC TATGAGGGAG GCTCTGTGAG CTGGCTGCCC 
CCAGCTATCT ACCGCAGCAC GTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG TTCCCAGACC TACAATGCTG AAGAGGTGGA GTTAATCTTT 
GCAGTGGATG ACGATGGCAA TGCCATCAAC AAAATTGACA TCGACACCGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT TGACTACTGC CCAGGCATGA TTCGCCATTA TGAGGGAGGC 
TCCACAGAAG ACCCTGGAGA AACTGACGTC ATCTACACGC TCATCATCCG TCGAAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGTGTGCTCA TTTCTGGCTT GGTGCTACTC 
GCTTACTTCC TACCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTCCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCGCTGC TGGGCAGGTA TCTTATTTTT GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG CGAACGCCAA CGACTCACGC CACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTACTG CCGCGCCTCC TCGGCTTGAG CCCACCCCCA 
GAGGATCCCG GAGCTGCCTC ACCAGCGAGG CGTGCCTCAT CTGTGGGCAT TCTGCTTAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG ACAGAGGCAT 
CGGCATGGAA CTTGGACCGC AGCCGCCCTC TGCCAGAACC TGGGTGCTGC AGCCCCTGAA 
GTCCGCTGCT GTGTGGATGC TGTGAACTTT GTGGCTGAGA GCACAAGGGA CCAGGAAGCC 
ACTGGAGAGG AACTGTCTGA CTGGGTGCGT ATGGGGAAGG CCCTCGACAA TGTCTGTTTT 
TGGGCAGCGT TGGTGCTCTT CAGCGTCGGT TCTACGCTCA TCTTCCTTGG AGGTTACTTC 
AACCAAGTTC CTGATCTCCC CTACCCACCG TGCATCCAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrne ( XM_017597174.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452663.1 Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa33294
Clone ID Related Accession (Same CDS sequence)	XM_017597174.1
Accession Version	XM_017597174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acetylcholine receptor subunit epsilon isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGG CTCTGCTTGG GACCCTGCTT CTCCTGGCAC TCTTTGGCAG AAGCCAAGGC 
AAGAATGAAG AGCTGAGCCT GTATCACCAT CTCTTCGACA ATTATGACCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ATGAGAAAGA AGAGACTCTG ACCACCAGTG TCTGGATTGG AATTGAATGG 
CAAGACTATC GGCTCAACTT CAGCAAGGAC GATTTTGCAG GCGTAGAAAT CCTGCGGGTC 
CCTTCCGAAC ATGTATGGCT GCCAGAGATT GTTCTGGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACTGCAA TGTTCTGGTC TATGAGGGAG GCTCTGTGAG CTGGCTGCCC 
CCAGCTATCT ACCGCAGCAC GTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG TTCCCAGACC TACAATGCTG AAGAGGTGGA GTTCATCTTT 
GCAGTGGATG ACGATGGCAA TGCCATCAAC AAAATTGACA TCGACACCGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT TGACTACTGC CCAGGCATGA TTCGCCATTA TGAGGGAGGC 
TCCACAGAAG ACCCTGGAGA AACTGACGTC ATCTACACGC TCATCATCCG TCGAAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGTGTGCTCA TTTCTGGCTT GGTGCTACTC 
GCTTACTTCC TACCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTCCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCGCTGC TGGGCAGGTA TCTTATTTTT GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG CGAACGCCAA CGACTCACGC CACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTACTG CCGCGCCTCC TCGGCTTGAG CCCACCCCCA 
GAGGATCCCG GAGCTGCCTC ACCAGCGAGG CGTGCCTCAT CTGTGGGCAT TCTGCTTAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG ACAGAGGCAT 
CGGCATGGAA CTTGGACCGC CGCCCTCTGC CAGAACCTGA GTGCTGCAGC CCCTGAAGTC 
CGCTGCTGTG TGGATGCTGT GAACTTTGTG GCTGAGAGCA CAAGGGACCA GGAAGCCACT 
GGAGAGGAAC TGTCTGACTG GGTGCGTATG GGGAAGGCCC TCGACAATGT CTGTTTTTGG 
GCAGCGTTGG TGCTCTTCAG CGTCGGTTCT ACGCTCATCT TCCTTGGAGG TTACTTCAAC 
CAAGTTCCTG ATCTCCCCTA CCCACCGTGC ATCCAACCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452663.1
CDS	1961..3442
Translation	MTMALLGTLLLLALFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIEWQDYRLNFSKDDFAGVEILRVPSEHVWLPEIVLENNIDGQFGVAYDCNVLVYEGGSVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNAINKIDIDTAAFTENGEWAIDYCPGMIRHYEGGSTEDPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGLSPPPEDPGAASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAALCQNLSAAAPEVRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP

Target ORF information:

RefSeq Version	XM_017597174.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597174.1

ORF Insert Sequence:

ATGACAATGG CTCTGCTTGG GACCCTGCTT CTCCTGGCAC TCTTTGGCAG AAGCCAAGGC 
AAGAATGAAG AGCTGAGCCT GTATCACCAT CTCTTCGACA ATTATGACCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ATGAGAAAGA AGAGACTCTG ACCACCAGTG TCTGGATTGG AATTGAATGG 
CAAGACTATC GGCTCAACTT CAGCAAGGAC GATTTTGCAG GCGTAGAAAT CCTGCGGGTC 
CCTTCCGAAC ATGTATGGCT GCCAGAGATT GTTCTGGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACTGCAA TGTTCTGGTC TATGAGGGAG GCTCTGTGAG CTGGCTGCCC 
CCAGCTATCT ACCGCAGCAC GTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG TTCCCAGACC TACAATGCTG AAGAGGTGGA GTTCATCTTT 
GCAGTGGATG ACGATGGCAA TGCCATCAAC AAAATTGACA TCGACACCGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT TGACTACTGC CCAGGCATGA TTCGCCATTA TGAGGGAGGC 
TCCACAGAAG ACCCTGGAGA AACTGACGTC ATCTACACGC TCATCATCCG TCGAAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGTGTGCTCA TTTCTGGCTT GGTGCTACTC 
GCTTACTTCC TACCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTCCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCGCTGC TGGGCAGGTA TCTTATTTTT GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG CGAACGCCAA CGACTCACGC CACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTACTG CCGCGCCTCC TCGGCTTGAG CCCACCCCCA 
GAGGATCCCG GAGCTGCCTC ACCAGCGAGG CGTGCCTCAT CTGTGGGCAT TCTGCTTAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG ACAGAGGCAT 
CGGCATGGAA CTTGGACCGC CGCCCTCTGC CAGAACCTGA GTGCTGCAGC CCCTGAAGTC 
CGCTGCTGTG TGGATGCTGT GAACTTTGTG GCTGAGAGCA CAAGGGACCA GGAAGCCACT 
GGAGAGGAAC TGTCTGACTG GGTGCGTATG GGGAAGGCCC TCGACAATGT CTGTTTTTGG 
GCAGCGTTGG TGCTCTTCAG CGTCGGTTCT ACGCTCATCT TCCTTGGAGG TTACTTCAAC 
CAAGTTCCTG ATCTCCCCTA CCCACCGTGC ATCCAACCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrne ( XM_017597173.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452662.1 Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa43265
Clone ID Related Accession (Same CDS sequence)	XM_017597173.1
Accession Version	XM_017597173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acetylcholine receptor subunit epsilon isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGG CTCTGCTTGG GACCCTGCTT CTCCTGGCAC TCTTTGGCAG AAGCCAAGGC 
AAGAATGAAG AGCTGAGCCT GTATCACCAT CTCTTCGACA ATTATGACCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ATGAGAAAGA AGAGACTCTG ACCACCAGTG TCTGGATTGG AATTGAATGG 
CAAGACTATC GGCTCAACTT CAGCAAGGAC GATTTTGCAG GCGTAGAAAT CCTGCGGGTC 
CCTTCCGAAC ATGTATGGCT GCCAGAGATT GTTCTGGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACTGCAA TGTTCTGGTC TATGAGGGAG GCTCTGTGAG CTGGCTGCCC 
CCAGCTATCT ACCGCAGCAC GTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG TTCCCAGACC TACAATGCTG AAGAGGTGGA GTTCATCTTT 
GCAGTGGATG ACGATGGCAA TGCCATCAAC AAAATTGACA TCGACACCGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT TGACTACTGC CCAGGCATGA TTCGCCATTA TGAGGGAGGC 
TCCACAGAAG ACCCTGGAGA AACTGACGTC ATCTACACGC TCATCATCCG TCGAAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGTGTGCTCA TTTCTGGCTT GGTGCTACTC 
GCTTACTTCC TACCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTCCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCGCTGC TGGGCAGGTA TCTTATTTTT GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG CGAACGCCAA CGACTCACGC CACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTACTG CCGCGCCTCC TCGGCTTGAG CCCACCCCCA 
GAGGATCCCG GAGCTGCCTC ACCAGCGAGG CGTGCCTCAT CTGTGGGCAT TCTGCTTAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG ACAGAGGCAT 
CGGCATGGAA CTTGGACCGC AGCCGCCCTC TGCCAGAACC TGAGTGCTGC AGCCCCTGAA 
GTCCGCTGCT GTGTGGATGC TGTGAACTTT GTGGCTGAGA GCACAAGGGA CCAGGAAGCC 
ACTGGAGAGG AACTGTCTGA CTGGGTGCGT ATGGGGAAGG CCCTCGACAA TGTCTGTTTT 
TGGGCAGCGT TGGTGCTCTT CAGCGTCGGT TCTACGCTCA TCTTCCTTGG AGGTTACTTC 
AACCAAGTTC CTGATCTCCC CTACCCACCG TGCATCCAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452662.1
CDS	150..1634
Translation	MTMALLGTLLLLALFGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIEWQDYRLNFSKDDFAGVEILRVPSEHVWLPEIVLENNIDGQFGVAYDCNVLVYEGGSVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNAINKIDIDTAAFTENGEWAIDYCPGMIRHYEGGSTEDPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGLSPPPEDPGAASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAAALCQNLSAAAPEVRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP

Target ORF information:

RefSeq Version	XM_017597173.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597173.1

ORF Insert Sequence:

ATGACAATGG CTCTGCTTGG GACCCTGCTT CTCCTGGCAC TCTTTGGCAG AAGCCAAGGC 
AAGAATGAAG AGCTGAGCCT GTATCACCAT CTCTTCGACA ATTATGACCC AGAATGCCGG 
CCAGTTAGGA GACCTGAGGA CACTGTCACC ATCACCCTCA AGGTCACCCT AACCAACCTC 
ATCTCACTGA ATGAGAAAGA AGAGACTCTG ACCACCAGTG TCTGGATTGG AATTGAATGG 
CAAGACTATC GGCTCAACTT CAGCAAGGAC GATTTTGCAG GCGTAGAAAT CCTGCGGGTC 
CCTTCCGAAC ATGTATGGCT GCCAGAGATT GTTCTGGAAA ACAATATTGA TGGGCAGTTT 
GGAGTGGCCT ACGACTGCAA TGTTCTGGTC TATGAGGGAG GCTCTGTGAG CTGGCTGCCC 
CCAGCTATCT ACCGCAGCAC GTGCGCAGTG GAGGTCACCT ATTTCCCCTT TGACTGGCAG 
AACTGCTCTC TCATTTTTCG TTCCCAGACC TACAATGCTG AAGAGGTGGA GTTCATCTTT 
GCAGTGGATG ACGATGGCAA TGCCATCAAC AAAATTGACA TCGACACCGC AGCTTTTACC 
GAGAATGGAG AATGGGCCAT TGACTACTGC CCAGGCATGA TTCGCCATTA TGAGGGAGGC 
TCCACAGAAG ACCCTGGAGA AACTGACGTC ATCTACACGC TCATCATCCG TCGAAAGCCG 
CTTTTTTACG TCATTAACAT CATTGTGCCT TGTGTGCTCA TTTCTGGCTT GGTGCTACTC 
GCTTACTTCC TACCTGCGCA GGCTGGTGGC CAGAAATGCA CGGTCTCTAT CAACGTCCTG 
CTAGCCCAGA CTGTCTTCTT GTTCCTAATT GCCCAGAAAA TTCCAGAGAC TTCTCTGAGC 
GTGCCGCTGC TGGGCAGGTA TCTTATTTTT GTCATGGTGG TTGCCACGCT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTATCTTTG CGAACGCCAA CGACTCACGC CACATCCCCT 
CGGCTGCGCC AGATTTTATT AGAGCTACTG CCGCGCCTCC TCGGCTTGAG CCCACCCCCA 
GAGGATCCCG GAGCTGCCTC ACCAGCGAGG CGTGCCTCAT CTGTGGGCAT TCTGCTTAGA 
GCGGAGGAGC TCATCTTGAA AAAGCCGCGG AGCGAACTCG TGTTTGAGGG ACAGAGGCAT 
CGGCATGGAA CTTGGACCGC AGCCGCCCTC TGCCAGAACC TGAGTGCTGC AGCCCCTGAA 
GTCCGCTGCT GTGTGGATGC TGTGAACTTT GTGGCTGAGA GCACAAGGGA CCAGGAAGCC 
ACTGGAGAGG AACTGTCTGA CTGGGTGCGT ATGGGGAAGG CCCTCGACAA TGTCTGTTTT 
TGGGCAGCGT TGGTGCTCTT CAGCGTCGGT TCTACGCTCA TCTTCCTTGG AGGTTACTTC 
AACCAAGTTC CTGATCTCCC CTACCCACCG TGCATCCAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrne ( XM_017597171.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452660.1 Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X1, mRNA.

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CloneID	ORa57276
Clone ID Related Accession (Same CDS sequence)	XM_017597171.1
Accession Version	XM_017597171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acetylcholine receptor subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA TTCTTTTTTT TCTTTGTTTT TTCAGGACAG AATTTCTTTC TGTGCAACAA 
CCCTGGCTGT GCTGGAACTC GCTTAGTATA CCAGGCTGGC CTCAAACTCA CAGAGATCCC 
CCTGTCTCTG CCTCCTGTGT GCTGGGTTTA AAGGTGTACA TCACCGATGC CTGGCACTGT 
GATCATCATG GTGAGAAGCA GACAGGCAGA AGCCAAGGCA AGAATGAAGA GCTGAGCCTG 
TATCACCATC TCTTCGACAA TTATGACCCA GAATGCCGGC CAGTTAGGAG ACCTGAGGAC 
ACTGTCACCA TCACCCTCAA GGTCACCCTA ACCAACCTCA TCTCACTGAA TGAGAAAGAA 
GAGACTCTGA CCACCAGTGT CTGGATTGGA ATTGAATGGC AAGACTATCG GCTCAACTTC 
AGCAAGGACG ATTTTGCAGG CGTAGAAATC CTGCGGGTCC CTTCCGAACA TGTATGGCTG 
CCAGAGATTG TTCTGGAAAA CAATATTGAT GGGCAGTTTG GAGTGGCCTA CGACTGCAAT 
GTTCTGGTCT ATGAGGGAGG CTCTGTGAGC TGGCTGCCCC CAGCTATCTA CCGCAGCACG 
TGCGCAGTGG AGGTCACCTA TTTCCCCTTT GACTGGCAGA ACTGCTCTCT CATTTTTCGT 
TCCCAGACCT ACAATGCTGA AGAGGTGGAG TTCATCTTTG CAGTGGATGA CGATGGCAAT 
GCCATCAACA AAATTGACAT CGACACCGCA GCTTTTACCG AGAATGGAGA ATGGGCCATT 
GACTACTGCC CAGGCATGAT TCGCCATTAT GAGGGAGGCT CCACAGAAGA CCCTGGAGAA 
ACTGACGTCA TCTACACGCT CATCATCCGT CGAAAGCCGC TTTTTTACGT CATTAACATC 
ATTGTGCCTT GTGTGCTCAT TTCTGGCTTG GTGCTACTCG CTTACTTCCT ACCTGCGCAG 
GCTGGTGGCC AGAAATGCAC GGTCTCTATC AACGTCCTGC TAGCCCAGAC TGTCTTCTTG 
TTCCTAATTG CCCAGAAAAT TCCAGAGACT TCTCTGAGCG TGCCGCTGCT GGGCAGGTAT 
CTTATTTTTG TCATGGTGGT TGCCACGCTC ATTGTCATGA ATTGCGTCAT CGTGCTCAAC 
GTATCTTTGC GAACGCCAAC GACTCACGCC ACATCCCCTC GGCTGCGCCA GATTTTATTA 
GAGCTACTGC CGCGCCTCCT CGGCTTGAGC CCACCCCCAG AGGATCCCGG AGCTGCCTCA 
CCAGCGAGGC GTGCCTCATC TGTGGGCATT CTGCTTAGAG CGGAGGAGCT CATCTTGAAA 
AAGCCGCGGA GCGAACTCGT GTTTGAGGGA CAGAGGCATC GGCATGGAAC TTGGACCGCA 
GCCGCCCTCT GCCAGAACCT GAGTGCTGCA GCCCCTGAAG TCCGCTGCTG TGTGGATGCT 
GTGAACTTTG TGGCTGAGAG CACAAGGGAC CAGGAAGCCA CTGGAGAGGA ACTGTCTGAC 
TGGGTGCGTA TGGGGAAGGC CCTCGACAAT GTCTGTTTTT GGGCAGCGTT GGTGCTCTTC 
AGCGTCGGTT CTACGCTCAT CTTCCTTGGA GGTTACTTCA ACCAAGTTCC TGATCTCCCC 
TACCCACCGT GCATCCAACC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452660.1
CDS	94..1737
Translation	MSKILFFLCFFRTEFLSVQQPWLCWNSLSIPGWPQTHRDPPVSASCVLGLKVYITDAWHCDHHGEKQTGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIEWQDYRLNFSKDDFAGVEILRVPSEHVWLPEIVLENNIDGQFGVAYDCNVLVYEGGSVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNAINKIDIDTAAFTENGEWAIDYCPGMIRHYEGGSTEDPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGLSPPPEDPGAASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAAALCQNLSAAAPEVRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP

Target ORF information:

RefSeq Version	XM_017597171.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597171.1

ORF Insert Sequence:

ATGAGTAAAA TTCTTTTTTT TCTTTGTTTT TTCAGGACAG AATTTCTTTC TGTGCAACAA 
CCCTGGCTGT GCTGGAACTC GCTTAGTATA CCAGGCTGGC CTCAAACTCA CAGAGATCCC 
CCTGTCTCTG CCTCCTGTGT GCTGGGTTTA AAGGTGTACA TCACCGATGC CTGGCACTGT 
GATCATCATG GTGAGAAGCA GACAGGCAGA AGCCAAGGCA AGAATGAAGA GCTGAGCCTG 
TATCACCATC TCTTCGACAA TTATGACCCA GAATGCCGGC CAGTTAGGAG ACCTGAGGAC 
ACTGTCACCA TCACCCTCAA GGTCACCCTA ACCAACCTCA TCTCACTGAA TGAGAAAGAA 
GAGACTCTGA CCACCAGTGT CTGGATTGGA ATTGAATGGC AAGACTATCG GCTCAACTTC 
AGCAAGGACG ATTTTGCAGG CGTAGAAATC CTGCGGGTCC CTTCCGAACA TGTATGGCTG 
CCAGAGATTG TTCTGGAAAA CAATATTGAT GGGCAGTTTG GAGTGGCCTA CGACTGCAAT 
GTTCTGGTCT ATGAGGGAGG CTCTGTGAGC TGGCTGCCCC CAGCTATCTA CCGCAGCACG 
TGCGCAGTGG AGGTCACCTA TTTCCCCTTT GACTGGCAGA ACTGCTCTCT CATTTTTCGT 
TCCCAGACCT ACAATGCTGA AGAGGTGGAG TTCATCTTTG CAGTGGATGA CGATGGCAAT 
GCCATCAACA AAATTGACAT CGACACCGCA GCTTTTACCG AGAATGGAGA ATGGGCCATT 
GACTACTGCC CAGGCATGAT TCGCCATTAT GAGGGAGGCT CCACAGAAGA CCCTGGAGAA 
ACTGACGTCA TCTACACGCT CATCATCCGT CGAAAGCCGC TTTTTTACGT CATTAACATC 
ATTGTGCCTT GTGTGCTCAT TTCTGGCTTG GTGCTACTCG CTTACTTCCT ACCTGCGCAG 
GCTGGTGGCC AGAAATGCAC GGTCTCTATC AACGTCCTGC TAGCCCAGAC TGTCTTCTTG 
TTCCTAATTG CCCAGAAAAT TCCAGAGACT TCTCTGAGCG TGCCGCTGCT GGGCAGGTAT 
CTTATTTTTG TCATGGTGGT TGCCACGCTC ATTGTCATGA ATTGCGTCAT CGTGCTCAAC 
GTATCTTTGC GAACGCCAAC GACTCACGCC ACATCCCCTC GGCTGCGCCA GATTTTATTA 
GAGCTACTGC CGCGCCTCCT CGGCTTGAGC CCACCCCCAG AGGATCCCGG AGCTGCCTCA 
CCAGCGAGGC GTGCCTCATC TGTGGGCATT CTGCTTAGAG CGGAGGAGCT CATCTTGAAA 
AAGCCGCGGA GCGAACTCGT GTTTGAGGGA CAGAGGCATC GGCATGGAAC TTGGACCGCA 
GCCGCCCTCT GCCAGAACCT GAGTGCTGCA GCCCCTGAAG TCCGCTGCTG TGTGGATGCT 
GTGAACTTTG TGGCTGAGAG CACAAGGGAC CAGGAAGCCA CTGGAGAGGA ACTGTCTGAC 
TGGGTGCGTA TGGGGAAGGC CCTCGACAAT GTCTGTTTTT GGGCAGCGTT GGTGCTCTTC 
AGCGTCGGTT CTACGCTCAT CTTCCTTGGA GGTTACTTCA ACCAAGTTCC TGATCTCCCC 
TACCCACCGT GCATCCAACC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrne ( XM_017597172.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452661.1 Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57277
Clone ID Related Accession (Same CDS sequence)	XM_017597172.1
Accession Version	XM_017597172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acetylcholine receptor subunit epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA TTCTTTTTTT TCTTTGTTTT TTCAGGACAG AATTTCTTTC TGTGCAACAA 
CCCTGGCTGT GCTGGAACTC GCTTAGTATA CCAGGCTGGC CTCAAACTCA CAGAGATCCC 
CCTGTCTCTG CCTCCTGTGT GCTGGGTTTA AAGGTGTACA TCACCGATGC CTGGCACTGT 
GATCATCATG GTGAGAAGCA GACAGGCAGA AGCCAAGGCA AGAATGAAGA GCTGAGCCTG 
TATCACCATC TCTTCGACAA TTATGACCCA GAATGCCGGC CAGTTAGGAG ACCTGAGGAC 
ACTGTCACCA TCACCCTCAA GGTCACCCTA ACCAACCTCA TCTCACTGAA TGAGAAAGAA 
GAGACTCTGA CCACCAGTGT CTGGATTGGA ATTGAATGGC AAGACTATCG GCTCAACTTC 
AGCAAGGACG ATTTTGCAGG CGTAGAAATC CTGCGGGTCC CTTCCGAACA TGTATGGCTG 
CCAGAGATTG TTCTGGAAAA CAATATTGAT GGGCAGTTTG GAGTGGCCTA CGACTGCAAT 
GTTCTGGTCT ATGAGGGAGG CTCTGTGAGC TGGCTGCCCC CAGCTATCTA CCGCAGCACG 
TGCGCAGTGG AGGTCACCTA TTTCCCCTTT GACTGGCAGA ACTGCTCTCT CATTTTTCGT 
TCCCAGACCT ACAATGCTGA AGAGGTGGAG TTCATCTTTG CAGTGGATGA CGATGGCAAT 
GCCATCAACA AAATTGACAT CGACACCGCA GCTTTTACCG AGAATGGAGA ATGGGCCATT 
GACTACTGCC CAGGCATGAT TCGCCATTAT GAGGGAGGCT CCACAGAAGA CCCTGGAGAA 
ACTGACGTCA TCTACACGCT CATCATCCGT CGAAAGCCGC TTTTTTACGT CATTAACATC 
ATTGTGCCTT GTGTGCTCAT TTCTGGCTTG GTGCTACTCG CTTACTTCCT ACCTGCGCAG 
GCTGGTGGCC AGAAATGCAC GGTCTCTATC AACGTCCTGC TAGCCCAGAC TGTCTTCTTG 
TTCCTAATTG CCCAGAAAAT TCCAGAGACT TCTCTGAGCG TGCCGCTGCT GGGCAGGTAT 
CTTATTTTTG TCATGGTGGT TGCCACGCTC ATTGTCATGA ATTGCGTCAT CGTGCTCAAC 
GTATCTTTGC GAACGCCAAC GACTCACGCC ACATCCCCTC GGCTGCGCCA GATTTTATTA 
GAGCTACTGC CGCGCCTCCT CGGCTTGAGC CCACCCCCAG AGGATCCCGG AGCTGCCTCA 
CCAGCGAGGC GTGCCTCATC TGTGGGCATT CTGCTTAGAG CGGAGGAGCT CATCTTGAAA 
AAGCCGCGGA GCGAACTCGT GTTTGAGGGA CAGAGGCATC GGCATGGAAC TTGGACCGCC 
GCCCTCTGCC AGAACCTGAG TGCTGCAGCC CCTGAAGTCC GCTGCTGTGT GGATGCTGTG 
AACTTTGTGG CTGAGAGCAC AAGGGACCAG GAAGCCACTG GAGAGGAACT GTCTGACTGG 
GTGCGTATGG GGAAGGCCCT CGACAATGTC TGTTTTTGGG CAGCGTTGGT GCTCTTCAGC 
GTCGGTTCTA CGCTCATCTT CCTTGGAGGT TACTTCAACC AAGTTCCTGA TCTCCCCTAC 
CCACCGTGCA TCCAACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452661.1
CDS	95..1735
Translation	MSKILFFLCFFRTEFLSVQQPWLCWNSLSIPGWPQTHRDPPVSASCVLGLKVYITDAWHCDHHGEKQTGRSQGKNEELSLYHHLFDNYDPECRPVRRPEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIEWQDYRLNFSKDDFAGVEILRVPSEHVWLPEIVLENNIDGQFGVAYDCNVLVYEGGSVSWLPPAIYRSTCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFIFAVDDDGNAINKIDIDTAAFTENGEWAIDYCPGMIRHYEGGSTEDPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHATSPRLRQILLELLPRLLGLSPPPEDPGAASPARRASSVGILLRAEELILKKPRSELVFEGQRHRHGTWTAALCQNLSAAAPEVRCCVDAVNFVAESTRDQEATGEELSDWVRMGKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP

Target ORF information:

RefSeq Version	XM_017597172.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor nicotinic epsilon subunit (Chrne), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597172.1

ORF Insert Sequence:

ATGAGTAAAA TTCTTTTTTT TCTTTGTTTT TTCAGGACAG AATTTCTTTC TGTGCAACAA 
CCCTGGCTGT GCTGGAACTC GCTTAGTATA CCAGGCTGGC CTCAAACTCA CAGAGATCCC 
CCTGTCTCTG CCTCCTGTGT GCTGGGTTTA AAGGTGTACA TCACCGATGC CTGGCACTGT 
GATCATCATG GTGAGAAGCA GACAGGCAGA AGCCAAGGCA AGAATGAAGA GCTGAGCCTG 
TATCACCATC TCTTCGACAA TTATGACCCA GAATGCCGGC CAGTTAGGAG ACCTGAGGAC 
ACTGTCACCA TCACCCTCAA GGTCACCCTA ACCAACCTCA TCTCACTGAA TGAGAAAGAA 
GAGACTCTGA CCACCAGTGT CTGGATTGGA ATTGAATGGC AAGACTATCG GCTCAACTTC 
AGCAAGGACG ATTTTGCAGG CGTAGAAATC CTGCGGGTCC CTTCCGAACA TGTATGGCTG 
CCAGAGATTG TTCTGGAAAA CAATATTGAT GGGCAGTTTG GAGTGGCCTA CGACTGCAAT 
GTTCTGGTCT ATGAGGGAGG CTCTGTGAGC TGGCTGCCCC CAGCTATCTA CCGCAGCACG 
TGCGCAGTGG AGGTCACCTA TTTCCCCTTT GACTGGCAGA ACTGCTCTCT CATTTTTCGT 
TCCCAGACCT ACAATGCTGA AGAGGTGGAG TTCATCTTTG CAGTGGATGA CGATGGCAAT 
GCCATCAACA AAATTGACAT CGACACCGCA GCTTTTACCG AGAATGGAGA ATGGGCCATT 
GACTACTGCC CAGGCATGAT TCGCCATTAT GAGGGAGGCT CCACAGAAGA CCCTGGAGAA 
ACTGACGTCA TCTACACGCT CATCATCCGT CGAAAGCCGC TTTTTTACGT CATTAACATC 
ATTGTGCCTT GTGTGCTCAT TTCTGGCTTG GTGCTACTCG CTTACTTCCT ACCTGCGCAG 
GCTGGTGGCC AGAAATGCAC GGTCTCTATC AACGTCCTGC TAGCCCAGAC TGTCTTCTTG 
TTCCTAATTG CCCAGAAAAT TCCAGAGACT TCTCTGAGCG TGCCGCTGCT GGGCAGGTAT 
CTTATTTTTG TCATGGTGGT TGCCACGCTC ATTGTCATGA ATTGCGTCAT CGTGCTCAAC 
GTATCTTTGC GAACGCCAAC GACTCACGCC ACATCCCCTC GGCTGCGCCA GATTTTATTA 
GAGCTACTGC CGCGCCTCCT CGGCTTGAGC CCACCCCCAG AGGATCCCGG AGCTGCCTCA 
CCAGCGAGGC GTGCCTCATC TGTGGGCATT CTGCTTAGAG CGGAGGAGCT CATCTTGAAA 
AAGCCGCGGA GCGAACTCGT GTTTGAGGGA CAGAGGCATC GGCATGGAAC TTGGACCGCC 
GCCCTCTGCC AGAACCTGAG TGCTGCAGCC CCTGAAGTCC GCTGCTGTGT GGATGCTGTG 
AACTTTGTGG CTGAGAGCAC AAGGGACCAG GAAGCCACTG GAGAGGAACT GTCTGACTGG 
GTGCGTATGG GGAAGGCCCT CGACAATGTC TGTTTTTGGG CAGCGTTGGT GCTCTTCAGC 
GTCGGTTCTA CGCTCATCTT CCTTGGAGGT TACTTCAACC AAGTTCCTGA TCTCCCCTAC 
CCACCGTGCA TCCAACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The neuromuscular junction: selective remodeling of synaptic regulators at the nerve/muscle interface.
Mechanisms of development130(6-8)402-11(2013 Jun-Aug)
Witzemann V,Chevessier F,Pacifici PG,Yampolsky P

A novel mode-of-action mediated by the fetal muscle nicotinic acetylcholine receptor resulting in developmental toxicity in rats.
Toxicological sciences : an official journal of the Society of Toxicology127(2)522-34(2012 Jun)
Rasoulpour RJ,Ellis-Hutchings RG,Terry C,Millar NS,Zablotny CL,Gibb A,Marshall V,Collins T,Carney EW,Billington R

Mammalian nicotinic acetylcholine receptors: from structure to function.
Physiological reviews89(1)73-120(2009 Jan)
Albuquerque EX,Pereira EF,Alkondon M,Rogers SW

The acetylcholine receptor gamma-to-epsilon switch occurs in individual endplates.
Biochemical and biophysical research communications331(4)1522-7(2005 Jun)
Yumoto N,Wakatsuki S,Sehara-Fujisawa A

Aberrant development of motor axons and neuromuscular synapses in MyoD-null mice.
The Journal of neuroscience : the official journal of the Society for Neuroscience23(12)5161-9(2003 Jun)
Wang ZZ,Washabaugh CH,Yao Y,Wang JM,Zhang L,Ontell MP,Watkins SC,Rudnicki MA,Ontell M

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Chrne cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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