Rpl27a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rpl27a
Entrez Gene ID	293418
Full Name	ribosomal protein L27a
General protein information	Preferred Names ribosomal protein L27a Names 60S ribosomal protein L27a
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q33

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106290.1	NP_001099760.1	60S ribosomal protein L27a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RPL27A	NP_001029256.1
Rattus norvegicus (Norway rat)	Rpl27a	NP_001099760.1
Danio rerio (zebrafish)	rpl27a	NP_956324.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011706	XP_320804.4
Magnaporthe oryzae (rice blast fungus)	MGG_06721	XP_003709432.1
Arabidopsis thaliana (thale cress)	RPL27AB	NP_173743.1
Oryza sativa (rice)	Os02g0175600	NP_001046056.1
	RPL27A	XP_002799693.1
Kluyveromyces lactis	KLLA0F06831g	XP_455390.1
Schizosaccharomyces pombe (fission yeast)	rpl2801	NP_596326.1
Neurospora crassa	NCU03806	XP_961195.2
Homo sapiens (human)	RPL27A	NP_000981.1
Canis lupus familiaris (dog)	RPL27A	NP_001239154.1
Mus musculus (house mouse)	Rpl27a	NP_036105.2
Schizosaccharomyces pombe (fission yeast)	rpl2802	NP_587907.1
Arabidopsis thaliana (thale cress)	AT1G70600	NP_177217.1
Bos taurus (cattle)	RPL27A	NP_001019642.1
Caenorhabditis elegans (roundworm)	Y37E3.8	NP_490927.1
Saccharomyces cerevisiae (baker's yeast)	RPL28	NP_011412.1
Oryza sativa (rice)	Os07g0613200	NP_001060254.1
Oryza sativa (rice)	Os03g0408300	NP_001050337.1
Gallus gallus (chicken)	RPL27A	NP_001264592.1
Drosophila melanogaster (fruit fly)	RpL27A	NP_001188689.2
Eremothecium gossypii	AGOS_AFL035C	NP_985513.1
Xenopus tropicalis (tropical clawed frog)	rpl27a	NP_001123801.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human and Drosophila 80S ribosome.
Anger AM, Armache JP, Berninghausen O, Habeck M, Subklewe M, Wilson DN, Beckmann R
Nature497(7447)80-5(2013 May)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing.
Odintsova TI, M?ller EC, Ivanov AV, Egorov TA, Bienert R, Vladimirov SN, Kostka S, Otto A, Wittmann-Liebold B, Karpova GG
Journal of protein chemistry22(3)249-58(2003 Apr)

Functional substitution of mouse ribosomal protein L27' for yeast ribosomal protein L29 in yeast ribosomes.
Fleming G, Belhumeur P, Skup D, Fried HM
Proceedings of the National Academy of Sciences of the United States of America86(1)217-21(1989 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rpl27a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rpl27a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00731	NM_001106290.1 Latest version!	Rattus norvegicus ribosomal protein L27a (Rpl27a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rpl27a ( NM_001106290.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099760.1 Rattus norvegicus ribosomal protein L27a (Rpl27a), mRNA.

Price & Availability↑

CloneID	ORa00731
Clone ID Related Accession (Same CDS sequence)	NM_001106290.1
Accession Version	NM_001106290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	60S ribosomal protein L27a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473956.2. On or before Oct 4, 2007 this sequence version replaced XM_215041.4, XM_001077420.1. ##Evidence-Data-START## Transcript exon combination :: CB313343.1, FQ144030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCATCCA GACTGAGGAA GACCCGGAAA CTCCGGGGCC ACGTGAGCCA CGGCCACGGT 
CGCATCGGTA AGCACCGCAA GCACCCAGGA GGCCGCGGGA ATGCTGGAGG CATGCATCAC 
CACAGGATCA ACTTTGACAA ATATCATCCA GGTTACTTCG GCAAAGTTGG CATGAGGCAT 
TACCACTTGA AGAGGAACCA GAGCTTCTGC CCAACTGTCA ACCTGGATAA ATTATGGACG 
TTGGTCAGCG AGCAGACACG GGTCAATGCA GCAAAAAACA AGAATGGAGT TGCTCCCATC 
ATTGACGTTG TTCGATCAGG CTACTACAAA GTTCTGGGGA AGGGGAAGCT CCCTAAGCAA 
CCTGTCATCG TGAAGGCCAA GTTCTTCAGC AGAAGAGCTG AAGAGAAGAT AAAGGGTGTG 
GGAGGTGCCT GTGTCCTGGT GGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099760.1
CDS	29..475
Translation	MPSRLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKNGVAPIIDVVRSGYYKVLGKGKLPKQPVIVKAKFFSRRAEEKIKGVGGACVLVA

Target ORF information:

RefSeq Version	NM_001106290.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ribosomal protein L27a (Rpl27a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106290.1

ORF Insert Sequence:

ATGCCATCCA GACTGAGGAA GACCCGGAAA CTCCGGGGCC ACGTGAGCCA CGGCCACGGT 
CGCATCGGTA AGCACCGCAA GCACCCAGGA GGCCGCGGGA ATGCTGGAGG CATGCATCAC 
CACAGGATCA ACTTTGACAA ATATCATCCA GGTTACTTCG GCAAAGTTGG CATGAGGCAT 
TACCACTTGA AGAGGAACCA GAGCTTCTGC CCAACTGTCA ACCTGGATAA ATTATGGACG 
TTGGTCAGCG AGCAGACACG GGTCAATGCA GCAAAAAACA AGAATGGAGT TGCTCCCATC 
ATTGACGTTG TTCGATCAGG CTACTACAAA GTTCTGGGGA AGGGGAAGCT CCCTAAGCAA 
CCTGTCATCG TGAAGGCCAA GTTCTTCAGC AGAAGAGCTG AAGAGAAGAT AAAGGGTGTG 
GGAGGTGCCT GTGTCCTGGT GGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human and Drosophila 80S ribosome.
Nature497(7447)80-5(2013 May)
Anger AM,Armache JP,Berninghausen O,Habeck M,Subklewe M,Wilson DN,Beckmann R

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing.
Journal of protein chemistry22(3)249-58(2003 Apr)
Odintsova TI,M?ller EC,Ivanov AV,Egorov TA,Bienert R,Vladimirov SN,Kostka S,Otto A,Wittmann-Liebold B,Karpova GG

Functional substitution of mouse ribosomal protein L27' for yeast ribosomal protein L29 in yeast ribosomes.
Proceedings of the National Academy of Sciences of the United States of America86(1)217-21(1989 Jan)
Fleming G,Belhumeur P,Skup D,Fried HM