Rasip1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasip1
Entrez Gene ID	292912
Full Name	Ras interacting protein 1
General protein information	Preferred Names Ras interacting protein 1 Names ras-interacting protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229030.3	XP_006229092.1	ras-interacting protein 1 isoform X1
NM_001106261.2	NP_001099731.1	ras-interacting protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rasip1	NP_082820.1
Rattus norvegicus (Norway rat)	Rasip1	NP_001099731.1
Danio rerio (zebrafish)	LOC100151130	XP_001921579.4
Canis lupus familiaris (dog)	RASIP1	XP_541512.3
Bos taurus (cattle)	RASIP1	NP_001180089.1
Xenopus tropicalis (tropical clawed frog)	rasip1	XP_004916310.1
Homo sapiens (human)	RASIP1	NP_060275.2

Gene Ontology
Bibliography

Related articles in PubMed

Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity.
de Kreuk BJ, Gingras AR, Knight JD, Liu JJ, Gingras AC, Ginsberg MH
eLife5e11394(2016 Jan)

Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC.
McKnight NC, Jefferies HB, Alemu EA, Saunders RE, Howell M, Johansen T, Tooze SA
The EMBO journal31(8)1931-46(2012 Apr)

Blood vessel tubulogenesis requires Rasip1 regulation of GTPase signaling.
Xu K, Sacharidou A, Fu S, Chong DC, Skaug B, Chen ZJ, Davis GE, Cleaver O
Developmental cell20(4)526-39(2011 Apr)

Rasip1 is required for endothelial cell motility, angiogenesis and vessel formation.
Xu K, Chong DC, Rankin SA, Zorn AM, Cleaver O
Developmental biology329(2)269-79(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rasip1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasip1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04520	NM_001106261.2 Latest version!	Rattus norvegicus Ras interacting protein 1 (Rasip1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
ORa22710	XM_006229030.3 Latest version!	Rattus norvegicus Ras interacting protein 1 (Rasip1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasip1 ( NM_001106261.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099731.1 Rattus norvegicus Ras interacting protein 1 (Rasip1), mRNA.

Price & Availability↑

CloneID	ORa04520
Clone ID Related Accession (Same CDS sequence)	NM_001106261.2
Accession Version	NM_001106261.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	ras-interacting protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168876.1. On Sep 4, 2008 this sequence version replaced NM_001106261.1. ##Evidence-Data-START## Transcript exon combination :: BC168876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCCAG GGGCGGCTGA TGCTCAGATG GTGCCAACGG ACCCTGGAGA CTTTGACCAG 
TTGACTCAGT GCCTCATTCA GGCACCCAGC AACCGTCCTT ACTTCCTTCT GCTCCAGGGC 
TACCAGGATG CTCAGGACTT CGTGGTGTAT GTGATGACAC GAGAGCAGCA CGTGTTCGGC 
CGAGGTGGGC CCTCGTCGTC CCGCGGCGGA TCCCCTGCCC CATATGTGGA CACCTTCCTC 
AACGCCCCAG ACATCCTGCC TCGACATTGC ACGGTGCGCG CGGGTCCTGA GCCCCCAGCT 
ATGGTGCGCC CATCCCGGGG CGCCCCGGTC ACACACAATG GCTGCCTTTT GCTGCGCGAG 
GCAGAGTTGC ATCCCGGAGA CCTGCTAGGC CTGGGAGAGC ATTTCCTGTT CATGTATAAG 
GACCCCCGCG CCGGGGGCTC TGGTCCCGCT CGGCCACCCT GGCTGCCTGC GCGCCCCGGG 
GCCGCGCCAC CGGGTCCGGG CTGGGCCTTT TCTTGTCGCC TGTGTGGCCG TGGCCTGCAG 
GAGCGCGGCG AGGCACTGGC TGCCTACCTG GACGGCCGCG AGCCGGTGCT GCGCTTTAGA 
CCACGAGAGG AAGAGGCGCT GTTGGGAGAG ATCGTGCGCG CTGCAGCCTC GGGCGCAGGA 
GACCTGCCAC CACTCGGGCC CGCCACTCTG CTGGCACTGT GTGTGCAGCA TTCGGCGAGG 
GAGCTAGAGC TGGGCCACCT GCCTCGCTTG CTGGGCCGCC TGGCCCGGCT CATCAAAGAG 
GCCGTTTGGG AAAAAATTAA AGAAATTGGA GACCGCCAGC CAGAGAACCA CCCAGAGGGA 
GTTCCTGAGG TGCCTCTGAC CCCAGAGGCT GTGTCTGTGG AGCTGAGACC GCTCATACTG 
TGGATGGCCA ATACCACAGA GCTTCTGAGC TTTGTTCAGG AGAAAGTGCT AGAGATGGAG 
AAAGAGGCTG ACCAGGAGGG CCTGTCCTCC GACCCTCAGC TCTGCAATGA TTTGGAATTG 
TGTGACGAGG CCTTGGCCCT CCTGGATGAG GTCATCATGT GTACCTTCCA GCAGTCCGTC 
TACTACCTCA CCAAGACTCT CTATTCGACA CTGCCAGCTC TCCTGGACAG TAACCCTTTC 
ACTGCCGGGG CAGAGTTGCC GGGGCCTGGT GCAGAGCTGG AGGCCATGCC ACCTGGACTC 
AGGCCTACCC TGGGTGTGTT CCAGGCAGCT CTAGAACTGA CCAGTCAGTG TGAGCTGCAT 
CCCGACCTCG TGTCCCAGAC TTTTGGCTAC TTGTTCTTCT TCTCCAATGC ATCCCTTCTC 
AACTCGCTGA TGGAACGAGG TCAAGGCCGA CCTTTCTATC AGTGGTCTCG AGCCGTCCAA 
ATCAGAACCA ACTTGGATCT CGTTTTGGAT TGGCTGCAGG GGGCCGGGCT GGGTGACATT 
GCCACTGAGT TCTTCCGGAA GCTCTCCATT GCCGTGAACC TGCTGTGTGT ACCCCGTACC 
TCCCTGCTCA AGGCTTCATG GAGCAGCTTA CGGACTGACT ACCCCACCTT GACTCCTGCT 
CAACTCCACC ATCTCCTCAG CCACTACCAG TTGGGTCCTG GCCGCGGACC ACCACCAGCT 
TGGGACCCAC CACCTGCAGA GCGAGATGCT GTGGACACAG GGGATATCTT TGAAAGTTTT 
TCCTCGCATC CTCCTCTCAT CCTACCCCTG GGTAGCTCAC GCCTGCGCCT CACGGGCCCA 
GTGACAGATG ATGCATTACA CCGAGAACTA CGCAGGCTCC GTCGCCTCCT CTGGGATCTT 
GAGCAGCAGG AGCTGCCAGC CAATCATCGC CATGGACCGC CGGTGGCTGC GACACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099731.1
CDS	264..2123
Translation	MAPGAADAQMVPTDPGDFDQLTQCLIQAPSNRPYFLLLQGYQDAQDFVVYVMTREQHVFGRGGPSSSRGGSPAPYVDTFLNAPDILPRHCTVRAGPEPPAMVRPSRGAPVTHNGCLLLREAELHPGDLLGLGEHFLFMYKDPRAGGSGPARPPWLPARPGAAPPGPGWAFSCRLCGRGLQERGEALAAYLDGREPVLRFRPREEEALLGEIVRAAASGAGDLPPLGPATLLALCVQHSARELELGHLPRLLGRLARLIKEAVWEKIKEIGDRQPENHPEGVPEVPLTPEAVSVELRPLILWMANTTELLSFVQEKVLEMEKEADQEGLSSDPQLCNDLELCDEALALLDEVIMCTFQQSVYYLTKTLYSTLPALLDSNPFTAGAELPGPGAELEAMPPGLRPTLGVFQAALELTSQCELHPDLVSQTFGYLFFFSNASLLNSLMERGQGRPFYQWSRAVQIRTNLDLVLDWLQGAGLGDIATEFFRKLSIAVNLLCVPRTSLLKASWSSLRTDYPTLTPAQLHHLLSHYQLGPGRGPPPAWDPPPAERDAVDTGDIFESFSSHPPLILPLGSSRLRLTGPVTDDALHRELRRLRRLLWDLEQQELPANHRHGPPVAATP

Target ORF information:

RefSeq Version	NM_001106261.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ras interacting protein 1 (Rasip1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106261.2

ORF Insert Sequence:

ATGGCCCCAG GGGCGGCTGA TGCTCAGATG GTGCCAACGG ACCCTGGAGA CTTTGACCAG 
TTGACTCAGT GCCTCATTCA GGCACCCAGC AACCGTCCTT ACTTCCTTCT GCTCCAGGGC 
TACCAGGATG CTCAGGACTT CGTGGTGTAT GTGATGACAC GAGAGCAGCA CGTGTTCGGC 
CGAGGTGGGC CCTCGTCGTC CCGCGGCGGA TCCCCTGCCC CATATGTGGA CACCTTCCTC 
AACGCCCCAG ACATCCTGCC TCGACATTGC ACGGTGCGCG CGGGTCCTGA GCCCCCAGCT 
ATGGTGCGCC CATCCCGGGG CGCCCCGGTC ACACACAATG GCTGCCTTTT GCTGCGCGAG 
GCAGAGTTGC ATCCCGGAGA CCTGCTAGGC CTGGGAGAGC ATTTCCTGTT CATGTATAAG 
GACCCCCGCG CCGGGGGCTC TGGTCCCGCT CGGCCACCCT GGCTGCCTGC GCGCCCCGGG 
GCCGCGCCAC CGGGTCCGGG CTGGGCCTTT TCTTGTCGCC TGTGTGGCCG TGGCCTGCAG 
GAGCGCGGCG AGGCACTGGC TGCCTACCTG GACGGCCGCG AGCCGGTGCT GCGCTTTAGA 
CCACGAGAGG AAGAGGCGCT GTTGGGAGAG ATCGTGCGCG CTGCAGCCTC GGGCGCAGGA 
GACCTGCCAC CACTCGGGCC CGCCACTCTG CTGGCACTGT GTGTGCAGCA TTCGGCGAGG 
GAGCTAGAGC TGGGCCACCT GCCTCGCTTG CTGGGCCGCC TGGCCCGGCT CATCAAAGAG 
GCCGTTTGGG AAAAAATTAA AGAAATTGGA GACCGCCAGC CAGAGAACCA CCCAGAGGGA 
GTTCCTGAGG TGCCTCTGAC CCCAGAGGCT GTGTCTGTGG AGCTGAGACC GCTCATACTG 
TGGATGGCCA ATACCACAGA GCTTCTGAGC TTTGTTCAGG AGAAAGTGCT AGAGATGGAG 
AAAGAGGCTG ACCAGGAGGG CCTGTCCTCC GACCCTCAGC TCTGCAATGA TTTGGAATTG 
TGTGACGAGG CCTTGGCCCT CCTGGATGAG GTCATCATGT GTACCTTCCA GCAGTCCGTC 
TACTACCTCA CCAAGACTCT CTATTCGACA CTGCCAGCTC TCCTGGACAG TAACCCTTTC 
ACTGCCGGGG CAGAGTTGCC GGGGCCTGGT GCAGAGCTGG AGGCCATGCC ACCTGGACTC 
AGGCCTACCC TGGGTGTGTT CCAGGCAGCT CTAGAACTGA CCAGTCAGTG TGAGCTGCAT 
CCCGACCTCG TGTCCCAGAC TTTTGGCTAC TTGTTCTTCT TCTCCAATGC ATCCCTTCTC 
AACTCGCTGA TGGAACGAGG TCAAGGCCGA CCTTTCTATC AGTGGTCTCG AGCCGTCCAA 
ATCAGAACCA ACTTGGATCT CGTTTTGGAT TGGCTGCAGG GGGCCGGGCT GGGTGACATT 
GCCACTGAGT TCTTCCGGAA GCTCTCCATT GCCGTGAACC TGCTGTGTGT ACCCCGTACC 
TCCCTGCTCA AGGCTTCATG GAGCAGCTTA CGGACTGACT ACCCCACCTT GACTCCTGCT 
CAACTCCACC ATCTCCTCAG CCACTACCAG TTGGGTCCTG GCCGCGGACC ACCACCAGCT 
TGGGACCCAC CACCTGCAGA GCGAGATGCT GTGGACACAG GGGATATCTT TGAAAGTTTT 
TCCTCGCATC CTCCTCTCAT CCTACCCCTG GGTAGCTCAC GCCTGCGCCT CACGGGCCCA 
GTGACAGATG ATGCATTACA CCGAGAACTA CGCAGGCTCC GTCGCCTCCT CTGGGATCTT 
GAGCAGCAGG AGCTGCCAGC CAATCATCGC CATGGACCGC CGGTGGCTGC GACACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasip1 ( XM_006229030.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229092.1 Rattus norvegicus Ras interacting protein 1 (Rasip1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22710
Clone ID Related Accession (Same CDS sequence)	XM_006229030.3
Accession Version	XM_006229030.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ras-interacting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229030.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTATCTG GTGAACGAAA GGAGGGCGGA AGCCCCCGCT TTGGGAAGCT CCATCTCCCT 
GTGGGCCTGT GGATCAATTC CCCAAGGAAA CAGCTCGCCA AACTGGGGCG GCGCTGGCCT 
AGCGCTGCCT CTGTCAAGTC CTCGTCCTCC GACACGGGGA GCCGCAGCAG CGAGCCCCTG 
CCGCCACCGC CGCCGCCGCA CGTGGAGCTG CGGCGCGTTG GTGCGGTGAA GGCGGCGGGG 
GGCGCCTCGG GCAGCCGAGC CAAGCGCATC TCGCAGCTGT TTCGGGGCTC GGGGGCCGGC 
GGCGCCGGGG GTCCTGGCAC GCCCGGGGGC GCGCAGCGCT GGGCCAGCGA GAAGAAGCTG 
CCTGAACTGG CGGCGGGTGT TGCTCCGGAG CCCCCGTTGC CCACGCGCGC CGCGGTGCCC 
CCGGGAGTGC TCAAGATCTT CGCGGCCGGG CTGGCGTCGG GCGCCAACTA CAAGAGCGTG 
CTGGCTACGG AGCGATCTAC CGCGCGCGAG CTGGTGGCGG AGGCGCTGGA GCGCTACGGG 
TTGACGGGCG GCCGCGGAGC GGGCGACAGC GGCTGCGTGG ACGCCTACGC GCTTTGCGAC 
GCTCTGGGCC GACCCGCCGC TGGCGTGGGC GGTGGCGAGT GGCGCGCGGA GCACCTGCGG 
GTGCTGGCCG ACGCCGAGCG CCCCCTGCTG GTGCAGGACC TGTGGCGCGC GCGGCCGGGC 
TGGGCGCGGC GCTTTGAGCT GCGCGGCCGC GAGGAGGCCC GTCGGCTGGA GCAGGAGGCC 
TTCGGTGCTG CGGATGCAGA CGGCACAAAC GCCCCATCTT GGCGGACCCA GAAGAATAGG 
TCTCGGGCAG CATCTGGCGG GGCAGCTTTG GCCAGTCCTG GTCCAGGATC AGGATCCGGG 
ACCCCCACTG GGTCCGGGGG CAAGGAGCGA TCTGAAAATC TGTCGCTGCG TCGAAGTGTG 
TCTGAGCTGA GTCTACAGGG GCGGAGGCGG AGGCAGCAGG AACGCAGGCA GCAGGCACTT 
AGCATGGCCC CAGGGGCGGC TGATGCTCAG ATGGTGCCAA CGGACCCTGG AGACTTTGAC 
CAGTTGACTC AGTGCCTCAT TCAGGCACCC AGCAACCGTC CTTACTTCCT TCTGCTCCAG 
GGCTACCAGG ATGCTCAGGA CTTCGTGGTG TATGTGATGA CACGAGAGCA GCACGTGTTC 
GGCCGAGGTG GGCCCTCGTC GTCCCGCGGC GGATCCCCTG CCCCATATGT GGACACCTTC 
CTCAACGCCC CAGACATCCT GCCTCGACAT TGCACGGTGC GCGCGGGTCC TGAGCCCCCA 
GCTATGGTGC GCCCATCCCG GGGCGCCCCG GTCACACACA ATGGCTGCCT TTTGCTGCGC 
GAGGCAGAGT TGCATCCCGG AGACCTGCTA GGCCTGGGAG AGCATTTCCT GTTCATGTAT 
AAGGACCCCC GCGCCGGGGG CTCTGGTCCC GCTCGGCCAC CCTGGCTGCC TGCGCGCCCC 
GGGGCCGCGC CACCGGGTCC GGGCTGGGCC TTTTCTTGTC GCCTGTGTGG CCGTGGCCTG 
CAGGAGCGCG GCGAGGCACT GGCTGCCTAC CTGGACGGCC GCGAGCCGGT GCTGCGCTTT 
AGACCACGAG AGGAAGAGGC GCTGTTGGGA GAGATCGTGC GCGCTGCAGC CTCGGGCGCA 
GGAGACCTGC CACCACTCGG GCCCGCCACT CTGCTGGCAC TGTGTGTGCA GCATTCGGCG 
AGGGAGCTAG AGCTGGGCCA CCTGCCTCGC TTGCTGGGCC GCCTGGCCCG GCTCATCAAA 
GAGGCCGTTT GGGAAAAAAT TAAAGAAATT GGAGACCGCC AGCCAGAGAA CCACCCAGAG 
GGAGTTCCTG AGGTGCCTCT GACCCCAGAG GCTGTGTCTG TGGAGCTGAG ACCGCTCATA 
CTGTGGATGG CCAATACCAC AGAGCTTCTG AGCTTTGTTC AGGAGAAAGT GCTAGAGATG 
GAGAAAGAGG CTGACCAGGA GGGCCTGTCC TCCGACCCTC AGCTCTGCAA TGATTTGGAA 
TTGTGTGACG AGGCCTTGGC CCTCCTGGAT GAGGTCATCA TGTGTACCTT CCAGCAGTCC 
GTCTACTACC TCACCAAGAC TCTCTATTCG ACACTGCCAG CTCTCCTGGA CAGTAACCCT 
TTCACTGCCG GGGCAGAGTT GCCGGGGCCT GGTGCAGAGC TGGAGGCCAT GCCACCTGGA 
CTCAGGCCTA CCCTGGGTGT GTTCCAGGCA GCTCTAGAAC TGACCAGTCA GTGTGAGCTG 
CATCCCGACC TCGTGTCCCA GACTTTTGGC TACTTGTTCT TCTTCTCCAA TGCATCCCTT 
CTCAACTCGC TGATGGAACG AGGTCAAGGC CGACCTTTCT ATCAGTGGTC TCGAGCCGTC 
CAAATCAGAA CCAACTTGGA TCTCGTTTTG GATTGGCTGC AGGGGGCCGG GCTGGGTGAC 
ATTGCCACTG AGTTCTTCCG GAAGCTCTCC ATTGCCGTGA ACCTGCTGTG TGTACCCCGT 
ACCTCCCTGC TCAAGGCTTC ATGGAGCAGC TTACGGACTG ACTACCCCAC CTTGACTCCT 
GCTCAACTCC ACCATCTCCT CAGCCACTAC CAGTTGGGTC CTGGCCGCGG ACCACCACCA 
GCTTGGGACC CACCACCTGC AGAGCGAGAT GCTGTGGACA CAGGGGATAT CTTTGAAAGT 
TTTTCCTCGC ATCCTCCTCT CATCCTACCC CTGGGTAGCT CACGCCTGCG CCTCACGGGC 
CCAGTGACAG ATGATGCATT ACACCGAGAA CTACGCAGGC TCCGTCGCCT CCTCTGGGAT 
CTTGAGCAGC AGGAGCTGCC AGCCAATCAT CGCCATGGAC CGCCGGTGGC TGCGACACCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229092.1
CDS	112..2994
Translation	MLSGERKEGGSPRFGKLHLPVGLWINSPRKQLAKLGRRWPSAASVKSSSSDTGSRSSEPLPPPPPPHVELRRVGAVKAAGGASGSRAKRISQLFRGSGAGGAGGPGTPGGAQRWASEKKLPELAAGVAPEPPLPTRAAVPPGVLKIFAAGLASGANYKSVLATERSTARELVAEALERYGLTGGRGAGDSGCVDAYALCDALGRPAAGVGGGEWRAEHLRVLADAERPLLVQDLWRARPGWARRFELRGREEARRLEQEAFGAADADGTNAPSWRTQKNRSRAASGGAALASPGPGSGSGTPTGSGGKERSENLSLRRSVSELSLQGRRRRQQERRQQALSMAPGAADAQMVPTDPGDFDQLTQCLIQAPSNRPYFLLLQGYQDAQDFVVYVMTREQHVFGRGGPSSSRGGSPAPYVDTFLNAPDILPRHCTVRAGPEPPAMVRPSRGAPVTHNGCLLLREAELHPGDLLGLGEHFLFMYKDPRAGGSGPARPPWLPARPGAAPPGPGWAFSCRLCGRGLQERGEALAAYLDGREPVLRFRPREEEALLGEIVRAAASGAGDLPPLGPATLLALCVQHSARELELGHLPRLLGRLARLIKEAVWEKIKEIGDRQPENHPEGVPEVPLTPEAVSVELRPLILWMANTTELLSFVQEKVLEMEKEADQEGLSSDPQLCNDLELCDEALALLDEVIMCTFQQSVYYLTKTLYSTLPALLDSNPFTAGAELPGPGAELEAMPPGLRPTLGVFQAALELTSQCELHPDLVSQTFGYLFFFSNASLLNSLMERGQGRPFYQWSRAVQIRTNLDLVLDWLQGAGLGDIATEFFRKLSIAVNLLCVPRTSLLKASWSSLRTDYPTLTPAQLHHLLSHYQLGPGRGPPPAWDPPPAERDAVDTGDIFESFSSHPPLILPLGSSRLRLTGPVTDDALHRELRRLRRLLWDLEQQELPANHRHGPPVAATP

Target ORF information:

RefSeq Version	XM_006229030.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ras interacting protein 1 (Rasip1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229030.3

ORF Insert Sequence:

ATGCTATCTG GTGAACGAAA GGAGGGCGGA AGCCCCCGCT TTGGGAAGCT CCATCTCCCT 
GTGGGCCTGT GGATCAATTC CCCAAGGAAA CAGCTCGCCA AACTGGGGCG GCGCTGGCCT 
AGCGCTGCCT CTGTCAAGTC CTCGTCCTCC GACACGGGGA GCCGCAGCAG CGAGCCCCTG 
CCGCCACCGC CGCCGCCGCA CGTGGAGCTG CGGCGCGTTG GTGCGGTGAA GGCGGCGGGG 
GGCGCCTCGG GCAGCCGAGC CAAGCGCATC TCGCAGCTGT TTCGGGGCTC GGGGGCCGGC 
GGCGCCGGGG GTCCTGGCAC GCCCGGGGGC GCGCAGCGCT GGGCCAGCGA GAAGAAGCTG 
CCTGAACTGG CGGCGGGTGT TGCTCCGGAG CCCCCGTTGC CCACGCGCGC CGCGGTGCCC 
CCGGGAGTGC TCAAGATCTT CGCGGCCGGG CTGGCGTCGG GCGCCAACTA CAAGAGCGTG 
CTGGCTACGG AGCGATCTAC CGCGCGCGAG CTGGTGGCGG AGGCGCTGGA GCGCTACGGG 
TTGACGGGCG GCCGCGGAGC GGGCGACAGC GGCTGCGTGG ACGCCTACGC GCTTTGCGAC 
GCTCTGGGCC GACCCGCCGC TGGCGTGGGC GGTGGCGAGT GGCGCGCGGA GCACCTGCGG 
GTGCTGGCCG ACGCCGAGCG CCCCCTGCTG GTGCAGGACC TGTGGCGCGC GCGGCCGGGC 
TGGGCGCGGC GCTTTGAGCT GCGCGGCCGC GAGGAGGCCC GTCGGCTGGA GCAGGAGGCC 
TTCGGTGCTG CGGATGCAGA CGGCACAAAC GCCCCATCTT GGCGGACCCA GAAGAATAGG 
TCTCGGGCAG CATCTGGCGG GGCAGCTTTG GCCAGTCCTG GTCCAGGATC AGGATCCGGG 
ACCCCCACTG GGTCCGGGGG CAAGGAGCGA TCTGAAAATC TGTCGCTGCG TCGAAGTGTG 
TCTGAGCTGA GTCTACAGGG GCGGAGGCGG AGGCAGCAGG AACGCAGGCA GCAGGCACTT 
AGCATGGCCC CAGGGGCGGC TGATGCTCAG ATGGTGCCAA CGGACCCTGG AGACTTTGAC 
CAGTTGACTC AGTGCCTCAT TCAGGCACCC AGCAACCGTC CTTACTTCCT TCTGCTCCAG 
GGCTACCAGG ATGCTCAGGA CTTCGTGGTG TATGTGATGA CACGAGAGCA GCACGTGTTC 
GGCCGAGGTG GGCCCTCGTC GTCCCGCGGC GGATCCCCTG CCCCATATGT GGACACCTTC 
CTCAACGCCC CAGACATCCT GCCTCGACAT TGCACGGTGC GCGCGGGTCC TGAGCCCCCA 
GCTATGGTGC GCCCATCCCG GGGCGCCCCG GTCACACACA ATGGCTGCCT TTTGCTGCGC 
GAGGCAGAGT TGCATCCCGG AGACCTGCTA GGCCTGGGAG AGCATTTCCT GTTCATGTAT 
AAGGACCCCC GCGCCGGGGG CTCTGGTCCC GCTCGGCCAC CCTGGCTGCC TGCGCGCCCC 
GGGGCCGCGC CACCGGGTCC GGGCTGGGCC TTTTCTTGTC GCCTGTGTGG CCGTGGCCTG 
CAGGAGCGCG GCGAGGCACT GGCTGCCTAC CTGGACGGCC GCGAGCCGGT GCTGCGCTTT 
AGACCACGAG AGGAAGAGGC GCTGTTGGGA GAGATCGTGC GCGCTGCAGC CTCGGGCGCA 
GGAGACCTGC CACCACTCGG GCCCGCCACT CTGCTGGCAC TGTGTGTGCA GCATTCGGCG 
AGGGAGCTAG AGCTGGGCCA CCTGCCTCGC TTGCTGGGCC GCCTGGCCCG GCTCATCAAA 
GAGGCCGTTT GGGAAAAAAT TAAAGAAATT GGAGACCGCC AGCCAGAGAA CCACCCAGAG 
GGAGTTCCTG AGGTGCCTCT GACCCCAGAG GCTGTGTCTG TGGAGCTGAG ACCGCTCATA 
CTGTGGATGG CCAATACCAC AGAGCTTCTG AGCTTTGTTC AGGAGAAAGT GCTAGAGATG 
GAGAAAGAGG CTGACCAGGA GGGCCTGTCC TCCGACCCTC AGCTCTGCAA TGATTTGGAA 
TTGTGTGACG AGGCCTTGGC CCTCCTGGAT GAGGTCATCA TGTGTACCTT CCAGCAGTCC 
GTCTACTACC TCACCAAGAC TCTCTATTCG ACACTGCCAG CTCTCCTGGA CAGTAACCCT 
TTCACTGCCG GGGCAGAGTT GCCGGGGCCT GGTGCAGAGC TGGAGGCCAT GCCACCTGGA 
CTCAGGCCTA CCCTGGGTGT GTTCCAGGCA GCTCTAGAAC TGACCAGTCA GTGTGAGCTG 
CATCCCGACC TCGTGTCCCA GACTTTTGGC TACTTGTTCT TCTTCTCCAA TGCATCCCTT 
CTCAACTCGC TGATGGAACG AGGTCAAGGC CGACCTTTCT ATCAGTGGTC TCGAGCCGTC 
CAAATCAGAA CCAACTTGGA TCTCGTTTTG GATTGGCTGC AGGGGGCCGG GCTGGGTGAC 
ATTGCCACTG AGTTCTTCCG GAAGCTCTCC ATTGCCGTGA ACCTGCTGTG TGTACCCCGT 
ACCTCCCTGC TCAAGGCTTC ATGGAGCAGC TTACGGACTG ACTACCCCAC CTTGACTCCT 
GCTCAACTCC ACCATCTCCT CAGCCACTAC CAGTTGGGTC CTGGCCGCGG ACCACCACCA 
GCTTGGGACC CACCACCTGC AGAGCGAGAT GCTGTGGACA CAGGGGATAT CTTTGAAAGT 
TTTTCCTCGC ATCCTCCTCT CATCCTACCC CTGGGTAGCT CACGCCTGCG CCTCACGGGC 
CCAGTGACAG ATGATGCATT ACACCGAGAA CTACGCAGGC TCCGTCGCCT CCTCTGGGAT 
CTTGAGCAGC AGGAGCTGCC AGCCAATCAT CGCCATGGAC CGCCGGTGGC TGCGACACCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heart of glass anchors Rasip1 at endothelial cell-cell junctions to support vascular integrity.
eLife5e11394(2016 Jan)
de Kreuk BJ,Gingras AR,Knight JD,Liu JJ,Gingras AC,Ginsberg MH

Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC.
The EMBO journal31(8)1931-46(2012 Apr)
McKnight NC,Jefferies HB,Alemu EA,Saunders RE,Howell M,Johansen T,Tooze SA

Blood vessel tubulogenesis requires Rasip1 regulation of GTPase signaling.
Developmental cell20(4)526-39(2011 Apr)
Xu K,Sacharidou A,Fu S,Chong DC,Skaug B,Chen ZJ,Davis GE,Cleaver O

Rasip1 is required for endothelial cell motility, angiogenesis and vessel formation.
Developmental biology329(2)269-79(2009 May)
Xu K,Chong DC,Rankin SA,Zorn AM,Cleaver O

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Rasip1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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