Rnf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf4
Entrez Gene ID	29274
Full Name	ring finger protein 4
General protein information	Preferred Names ring finger protein 4 Names E3 ubiquitin-protein ligase RNF4 E3 ubiquitin ligase RNF4 RING-type E3 ubiquitin transferase RNF4 SNURF small nuclear ring finger protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21
Summary	may act as a coregulator for nuclear receptor mediated transcriptional activation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019182.2	NP_062055.1	E3 ubiquitin-protein ligase RNF4
XM_008770272.2	XP_008768494.1	E3 ubiquitin-protein ligase RNF4 isoform X1
XM_017599146.1	XP_017454635.1	E3 ubiquitin-protein ligase RNF4 isoform X1
XM_008770273.2	XP_008768495.1	E3 ubiquitin-protein ligase RNF4 isoform X1
XM_008770271.2	XP_008768493.1	E3 ubiquitin-protein ligase RNF4 isoform X1
XM_017599145.1	XP_017454634.1	E3 ubiquitin-protein ligase RNF4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF4	NP_001171938.1
Pan troglodytes (chimpanzee)	RNF4	NP_001238838.1
Danio rerio (zebrafish)	im:7142942	XP_683428.1
Xenopus tropicalis (tropical clawed frog)	rnf4	NP_001016554.1
Canis lupus familiaris (dog)	RNF4	XP_853173.1
Mus musculus (house mouse)	Rnf4	NP_035408.1
Bos taurus (cattle)	RNF4	NP_001040054.1
Rattus norvegicus (Norway rat)	Rnf4	NP_062055.1
Gallus gallus (chicken)	RNF4	NP_001012907.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains.
Branigan E, Plechanovov? A, Jaffray EG, Naismith JH, Hay RT
Nature structural & molecular biology22(8)597-602(2015 Aug)

RNF4 interacts with both SUMO and nucleosomes to promote the DNA damage response.
Groocock LM, Nie M, Prudden J, Moiani D, Wang T, Cheltsov A, Rambo RP, Arvai AS, Hitomi C, Tainer JA, Luger K, Perry JJ, Lazzerini-Denchi E, Boddy MN
EMBO reports15(5)601-8(2014 May)

The Nrf2 regulatory network provides an interface between redox and intermediary metabolism.
Hayes JD, Dinkova-Kostova AT
Trends in biochemical sciences39(4)199-218(2014 Apr)

Push back to respond better: regulatory inhibition of the DNA double-strand break response.
Panier S, Durocher D
Nature reviews. Molecular cell biology14(10)661-72(2013 Oct)

Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis.
Plechanovov? A, Jaffray EG, Tatham MH, Naismith JH, Hay RT
Nature489(7414)115-20(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that ubiquitin-conjugating enzyme E2 variant 2 (Ube2V2) and ring finger protein 4 (RNF4) together induce an active conformation of the ubiquitin-conjugating enzyme Ubc13-ubiquitin (Ubc13~Ub) thioester.
Title: Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains.

crystal structure of the dimeric RING domain of rat RNF4 in complex with E2 (UbcH5A) linked by an isopeptide bond to ubiquitin
Title: Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis.

The dimeric RING ligase RNF4 facilitates ubiquitin transfer by dimeric RING ligases preferentially binding the E2~ubiquitin thioester across the dimer and activating the thioester bond for catalysis.
Title: Mechanism of ubiquitylation by dimeric RING ligase RNF4.

Data show that the RING-domain-containing ubiquitin E3 ligase, RNF4 (also known as SNURF), targets poly-SUMO-modified proteins for degradation mediated by ubiquitin.
Title: RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation.

SNURF is involved in the regulation of processes required for late steps of spermatid maturation
Title: Expression of the nuclear RING finger protein SNURF/RNF4 during rat testis development suggests a role in spermatid maturation.

The following Rnf4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03959	NM_019182.2 Latest version!	Rattus norvegicus ring finger protein 4 (Rnf4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa03959	XM_017599146.1 Latest version!	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa03959	XM_008770272.2 Latest version!	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa03959	XM_017599145.1 Latest version!	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa03959	XM_008770271.2 Latest version!	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa03959	XM_008770273.2 Latest version!	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf4 ( NM_019182.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062055.1 Rattus norvegicus ring finger protein 4 (Rnf4), mRNA.

Price & Availability↑

CloneID	ORa03959
Clone ID Related Accession (Same CDS sequence)	NM_019182.2 , XM_008770272.2 , XM_017599146.1 , XM_008770273.2 , XM_008770271.2 , XM_017599145.1
Accession Version	NM_019182.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062024.1. On Jun 10, 2007 this sequence version replaced NM_019182.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062024.1, FN804513.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062055.1
CDS	272..856
Translation	MSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	NM_019182.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 4 (Rnf4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019182.2

ORF Insert Sequence:

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf4 ( XM_008770272.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768494.1 Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa03959
Clone ID Related Accession (Same CDS sequence)	NM_019182.2 , XM_008770272.2 , XM_017599146.1 , XM_008770273.2 , XM_008770271.2 , XM_017599145.1
Accession Version	XM_008770272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768494.1
CDS	312..896
Translation	MSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	XM_008770272.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770272.2

ORF Insert Sequence:

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf4 ( XM_017599146.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454635.1 Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa03959
Clone ID Related Accession (Same CDS sequence)	NM_019182.2 , XM_008770272.2 , XM_017599146.1 , XM_008770273.2 , XM_008770271.2 , XM_017599145.1
Accession Version	XM_017599146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454635.1
CDS	306..890
Translation	MSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	XM_017599146.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599146.1

ORF Insert Sequence:

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf4 ( XM_008770273.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768495.1 Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa03959
Clone ID Related Accession (Same CDS sequence)	NM_019182.2 , XM_008770272.2 , XM_017599146.1 , XM_008770273.2 , XM_008770271.2 , XM_017599145.1
Accession Version	XM_008770273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768495.1
CDS	386..970
Translation	MSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	XM_008770273.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770273.2

ORF Insert Sequence:

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf4 ( XM_008770271.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768493.1 Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa03959
Clone ID Related Accession (Same CDS sequence)	NM_019182.2 , XM_008770272.2 , XM_017599146.1 , XM_008770273.2 , XM_008770271.2 , XM_017599145.1
Accession Version	XM_008770271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768493.1
CDS	381..965
Translation	MSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	XM_008770271.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770271.2

ORF Insert Sequence:

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf4 ( XM_017599145.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454634.1 Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X4, mRNA.

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CloneID	ORa03959
Clone ID Related Accession (Same CDS sequence)	NM_019182.2 , XM_008770272.2 , XM_017599146.1 , XM_008770273.2 , XM_008770271.2 , XM_017599145.1
Accession Version	XM_017599145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454634.1
CDS	290..874
Translation	MSTRNPQRKRRGGAVNSRQTQKRTRETTSTPEISLEAEPIELVETVGDEIVDLTCESLEPVVVDLTHNDSVVIVEERRRPRRNGRRLRQDHADSCVVSSDDEELSKDKDVYVTTHTPRSTKDEGTTGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

Target ORF information:

RefSeq Version	XM_017599145.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 4 (Rnf4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599145.1

ORF Insert Sequence:

ATGAGTACAA GAAATCCTCA AAGAAAGCGG CGTGGTGGAG CTGTGAATTC TAGACAAACC 
CAGAAGCGAA CTCGGGAAAC AACTTCAACC CCTGAGATTT CCTTGGAAGC AGAACCCATA 
GAACTTGTGG AAACCGTTGG AGATGAAATC GTGGACCTCA CCTGTGAATC TTTAGAGCCT 
GTGGTTGTGG ACCTGACTCA CAATGACTCT GTTGTGATTG TTGAAGAAAG GAGAAGGCCA 
AGGAGAAATG GGAGGAGGTT GCGCCAAGAC CATGCTGACA GCTGTGTGGT GAGCAGTGAT 
GATGAAGAAC TGTCTAAGGA CAAAGATGTA TACGTGACTA CCCATACTCC TCGAAGCACC 
AAGGATGAAG GAACTACTGG ACTTAGGCCC TCGGGCACCG TCAGTTGTCC GATCTGCATG 
GATGGATACT CTGAGATTGT GCAGAATGGG CGTCTCATTG TTTCTACAGA ATGTGGCCAC 
GTCTTCTGTA GCCAGTGCCT CCGTGATTCC CTTAAGAATG CTAACACTTG CCCAACTTGT 
AGGAAAAAGA TCAACCATAA ACGGTACCAT CCCATTTATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains.
Nature structural & molecular biology22(8)597-602(2015 Aug)
Branigan E,Plechanovov? A,Jaffray EG,Naismith JH,Hay RT

RNF4 interacts with both SUMO and nucleosomes to promote the DNA damage response.
EMBO reports15(5)601-8(2014 May)
Groocock LM,Nie M,Prudden J,Moiani D,Wang T,Cheltsov A,Rambo RP,Arvai AS,Hitomi C,Tainer JA,Luger K,Perry JJ,Lazzerini-Denchi E,Boddy MN

The Nrf2 regulatory network provides an interface between redox and intermediary metabolism.
Trends in biochemical sciences39(4)199-218(2014 Apr)
Hayes JD,Dinkova-Kostova AT

Push back to respond better: regulatory inhibition of the DNA double-strand break response.
Nature reviews. Molecular cell biology14(10)661-72(2013 Oct)
Panier S,Durocher D

Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis.
Nature489(7414)115-20(2012 Sep)
Plechanovov? A,Jaffray EG,Tatham MH,Naismith JH,Hay RT

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Rnf4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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