Cfl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cfl1
Entrez Gene ID	29271
Full Name	cofilin 1
General protein information	Preferred Names cofilin 1 Names cofilin-1 cofilin 1, non-muscle cofilin, non-muscle isoform
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	plays a role in nuclear import of actin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017147.2	NP_058843.1	cofilin-1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CFL1	NP_001015655.1
Pan troglodytes (chimpanzee)	CFL1	XP_003313184.1
Canis lupus familiaris (dog)	CFL1	XP_533231.1
Rattus norvegicus (Norway rat)	Cfl1	NP_058843.1
Xenopus tropicalis (tropical clawed frog)	cfl1	NP_998878.1
	CFL1	XP_002799522.1
Homo sapiens (human)	CFL1	NP_005498.1
Mus musculus (house mouse)	Cfl1	NP_031713.1

Gene Ontology
Bibliography

Related articles in PubMed

The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton.
Rogg M, Yasuda-Yamahara M, Abed A, Dinse P, Helmst?dter M, Conzelmann AC, Frimmel J, Sellung D, Biniossek ML, Kretz O, Grahammer F, Schilling O, Huber TB, Schell C
Scientific reports7(1)15910(2017 Nov)

c?Maf inducing protein inhibits cofilin?1 activity and alters podocyte cytoskeleton organization.
Yu L, Ye J, Liu Q, Feng J, Gu X, Sun Q, Lu G
Molecular medicine reports16(4)4955-4963(2017 Oct)

Topical administration of a Rock/Net inhibitor promotes retinal ganglion cell survival and axon regeneration after optic nerve injury.
Shaw PX, Sang A, Wang Y, Ho D, Douglas C, Dia L, Goldberg JL
Experimental eye research15833-42(2017 05)

The Rho kinase inhibitor, fasudil, ameliorates diabetes-induced cardiac dysfunction by improving calcium clearance and actin remodeling.
Lai D, Gao J, Bi X, He H, Shi X, Weng S, Chen Y, Yang Y, Ye Y, Fu G
Journal of molecular medicine (Berlin, Germany)95(2)155-165(2017 Feb)

Myoinositol Attenuates the Cell Loss and Biochemical Changes Induced by Kainic Acid Status Epilepticus.
Tsverava L, Lordkipanidze T, Lepsveridze E, Nozadze M, Kikvidze M, Solomonia R
BioMed research international20162794096(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cofilin1 is involved in hypertensive nephropathy by modulating the nuclear translocation of NF-kappaB and the expression of its downstream inflammatory factors in renal tubular epithelial cells
Title: Cofilin1 is involved in hypertension-induced renal damage via the regulation of NF-?B in renal tubular epithelial cells.

Cofilin1 regulates neocortical functional plasticity through the remodeling of dendritic spines within circuits that connect columns.
Title: Cofilin1 controls transcolumnar plasticity in dendritic spines in adult barrel cortex.

Data indicate that RNAi-mediated silencing of cofilin induces dendritic spine shrinkage.
Title: Activity-dependent dendritic spine shrinkage and growth involve downregulation of cofilin via distinct mechanisms.

Prevention of the hypertrophic response to adenosine receptor agonists by adenosine receptor activation is mediated by prevention of RhoA stimulation and actin polymerization.
Title: Prevention of RhoA activation and cofilin-mediated actin polymerization mediates the antihypertrophic effect of adenosine receptor agonists in angiotensin II- and endothelin-1-treated cardiomyocytes.

Although ADF and cofilin-1 have many redundant functions, each of these proteins has functional differences that affect F-actin structures, cell adhesion and lamellipodial dynamics, all of which are important determinants of cell migration.
Title: Non-overlapping activities of ADF and cofilin-1 during the migration of metastatic breast tumor cells.

The following Cfl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cfl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03029	NM_017147.2 Latest version!	Rattus norvegicus cofilin 1 (Cfl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cfl1 ( NM_017147.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058843.1 Rattus norvegicus cofilin 1 (Cfl1), mRNA.

Price & Availability↑

CloneID	ORa03029
Clone ID Related Accession (Same CDS sequence)	NM_017147.2
Accession Version	NM_017147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	cofilin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC059143.1. On Feb 9, 2004 this sequence version replaced NM_017147.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC059143.1, FQ214504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCTG GTGTGGCTGT CTCTGATGGA GTCATCAAGG TGTTCAATGA CATGAAAGTT 
CGCAAGTCTT CAACGCCAGA AGAAGTGAAG AAACGCAAGA AGGCAGTGCT CTTTTGCCTG 
AGTGAGGACA AGAAGAACAT CATCCTGGAG GAGGGCAAGG AGATTCTGGT AGGAGATGTG 
GGGCAGACTG TGGACGACCC CTACACCACT TTTGTCAAGA TGCTGCCAGA CAAGGACTGC 
CGCTATGCTC TCTATGACGC AACCTATGAG ACCAAGGAGA GCAAGAAGGA GGACCTGGTA 
TTCATTTTCT GGGCCCCCGA GAGTGCACCC CTTAAGAGCA AAATGATCTA TGCCAGCTCC 
AAGGATGCCA TCAAGAAGAA ACTGACAGGA ATCAAGCACG AATTACAAGC TAACTGCTAC 
GAGGAGGTCA AGGACCGCTG CACCCTGGCA GAGAAACTAG GTGGCAGCGC CGTCATTTCC 
CTGGAGGGCA AGCCTTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058843.1
CDS	82..582
Translation	MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYTTFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL

Target ORF information:

RefSeq Version	NM_017147.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cofilin 1 (Cfl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017147.2

ORF Insert Sequence:

ATGGCCTCTG GTGTGGCTGT CTCTGATGGA GTCATCAAGG TGTTCAATGA CATGAAAGTT 
CGCAAGTCTT CAACGCCAGA AGAAGTGAAG AAACGCAAGA AGGCAGTGCT CTTTTGCCTG 
AGTGAGGACA AGAAGAACAT CATCCTGGAG GAGGGCAAGG AGATTCTGGT AGGAGATGTG 
GGGCAGACTG TGGACGACCC CTACACCACT TTTGTCAAGA TGCTGCCAGA CAAGGACTGC 
CGCTATGCTC TCTATGACGC AACCTATGAG ACCAAGGAGA GCAAGAAGGA GGACCTGGTA 
TTCATTTTCT GGGCCCCCGA GAGTGCACCC CTTAAGAGCA AAATGATCTA TGCCAGCTCC 
AAGGATGCCA TCAAGAAGAA ACTGACAGGA ATCAAGCACG AATTACAAGC TAACTGCTAC 
GAGGAGGTCA AGGACCGCTG CACCCTGGCA GAGAAACTAG GTGGCAGCGC CGTCATTTCC 
CTGGAGGGCA AGCCTTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton.
Scientific reports7(1)15910(2017 Nov)
Rogg M,Yasuda-Yamahara M,Abed A,Dinse P,Helmst?dter M,Conzelmann AC,Frimmel J,Sellung D,Biniossek ML,Kretz O,Grahammer F,Schilling O,Huber TB,Schell C

c?Maf inducing protein inhibits cofilin?1 activity and alters podocyte cytoskeleton organization.
Molecular medicine reports16(4)4955-4963(2017 Oct)
Yu L,Ye J,Liu Q,Feng J,Gu X,Sun Q,Lu G

Topical administration of a Rock/Net inhibitor promotes retinal ganglion cell survival and axon regeneration after optic nerve injury.
Experimental eye research15833-42(2017 05)
Shaw PX,Sang A,Wang Y,Ho D,Douglas C,Dia L,Goldberg JL

The Rho kinase inhibitor, fasudil, ameliorates diabetes-induced cardiac dysfunction by improving calcium clearance and actin remodeling.
Journal of molecular medicine (Berlin, Germany)95(2)155-165(2017 Feb)
Lai D,Gao J,Bi X,He H,Shi X,Weng S,Chen Y,Yang Y,Ye Y,Fu G

Myoinositol Attenuates the Cell Loss and Biochemical Changes Induced by Kainic Acid Status Epilepticus.
BioMed research international20162794096(2016)
Tsverava L,Lordkipanidze T,Lepsveridze E,Nozadze M,Kikvidze M,Solomonia R

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Cfl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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