Nlrp12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nlrp12
Entrez Gene ID	292541
Full Name	NLR family, pyrin domain containing 12
Synonyms	Nalp12
General protein information	Preferred Names NLR family, pyrin domain containing 12 Names NACHT, LRR and PYD domains-containing protein 12 NACHT, leucine rich repeat and PYD containing 12
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_017588879.1	XP_017444368.1	NACHT, LRR and PYD domains-containing protein 12 isoform X1
XM_017588881.1	XP_017444370.1	NACHT, LRR and PYD domains-containing protein 12 isoform X3
XM_008758753.2	XP_008756975.1	NACHT, LRR and PYD domains-containing protein 12 isoform X2
XM_017588883.1	XP_017444372.1	NACHT, LRR and PYD domains-containing protein 12 isoform X5
XM_017588882.1	XP_017444371.1	NACHT, LRR and PYD domains-containing protein 12 isoform X4
NM_001169142.1	NP_001162613.1	NACHT, LRR and PYD domains-containing protein 12
XM_017588880.1	XP_017444369.1	NACHT, LRR and PYD domains-containing protein 12 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NLRP12	XP_524387.2
	NLRP12	XP_001118846.2
Bos taurus (cattle)	NLRP12	NP_001179480.1
Rattus norvegicus (Norway rat)	Nlrp12	NP_001162613.1
Homo sapiens (human)	NLRP12	NP_001264055.1
Canis lupus familiaris (dog)	NLRP12	XP_005616175.1
Canis lupus familiaris (dog)	LOC100855742	XP_005616257.1
Mus musculus (house mouse)	Nlrp12	NP_001028603.1

Gene Ontology
Bibliography

Related articles in PubMed

NLRP12 suppresses colon inflammation and tumorigenesis through the negative regulation of noncanonical NF-?B signaling.
Allen IC, Wilson JE, Schneider M, Lich JD, Roberts RA, Arthur JC, Woodford RM, Davis BK, Uronis JM, Herfarth HH, Jobin C, Rogers AB, Ting JP
Immunity36(5)742-54(2012 May)

Cutting edge: NLRP12 controls dendritic and myeloid cell migration to affect contact hypersensitivity.
Arthur JC, Lich JD, Ye Z, Allen IC, Gris D, Wilson JE, Schneider M, Roney KE, O'Connor BP, Moore CB, Morrison A, Sutterwala FS, Bertin J, Koller BH, Liu Z, Ting JP
Journal of immunology (Baltimore, Md. : 1950)185(8)4515-9(2010 Oct)

Mutations in NALP12 cause hereditary periodic fever syndromes.
J?ru I, Duquesnoy P, Fernandes-Alnemri T, Cochet E, Yu JW, Lackmy-Port-Lis M, Grimprel E, Landman-Parker J, Hentgen V, Marlin S, McElreavey K, Sarkisian T, Grateau G, Alnemri ES, Amselem S
Proceedings of the National Academy of Sciences of the United States of America105(5)1614-9(2008 Feb)

Monarch-1 suppresses non-canonical NF-kappaB activation and p52-dependent chemokine expression in monocytes.
Lich JD, Williams KL, Moore CB, Arthur JC, Davis BK, Taxman DJ, Ting JP
Journal of immunology (Baltimore, Md. : 1950)178(3)1256-60(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nlrp12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nlrp12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa56431	XM_017588881.1 Latest version!	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa56432	XM_017588882.1 Latest version!	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa56433	XM_017588883.1 Latest version!	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa56430	XM_017588880.1 Latest version!	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56430	XM_017588879.1 Latest version!	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa30178	NM_001169142.1 Latest version!	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa30178	XM_008758753.2 Latest version!	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nlrp12 ( XM_017588881.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444370.1 Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa56431
Clone ID Related Accession (Same CDS sequence)	XM_017588881.1
Accession Version	XM_017588881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCAGCTGAAG 
ATCTGCCACC TTGGACAAGC TTCCTGTGAA GATCTGGCTT CTACTCTCAA AATGAACCAG 
AGCCTGATGG AGCTGGACCT GGGTCTGAAT GACCTTGGGG ATTCTGGGGC GCTTCTGCTG 
TGTGAGGGCC TCAGGCATCC AGATTGCAAA CTCCAGACCC TCCGGCTGGG CATTTGCCGA 
CTGGGCTCAG ACGCGTGTGC AGGGGTTGCT AGTGTGCTCC AGGTCAACAC GTGCCTCCGA 
GAGCTGGACC TGAGCTTCAA TGACTTGGGA GACAGGGGCC TGTGGCTGCT GGGGGAAGGA 
CTGAGGCACC AGACCTGCAG ACTCCAGAAG CTCTGGCTGG ACAGTTGCGG CCTCACTTCC 
AAAGCATGTG AGGACCTGTC TTCAGTCCTG GGAATCAGTC AGACCCTGAA TGAGCTTTAT 
CTGACCAATA ATGCTCTGGG GGACACTGGT GTTCGTCTGC TGTGCAAGAG GCTGAGGCAT 
CCGGGGTGCA AGCTTCGTGT CCTGTGGCTT TTCGGAATGG ACCTGAACAA AGTGACCCAC 
AGGAGGATGG CGGCACTTCG AGTCACAAAA CCATACCTAG ATATTGGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444370.1
CDS	200..3193
Translation	MLPATAKDALHRLSTYLEELEAGELKKFKLYLGIAEELGQDKIPRGRMEMAGPLEMAQLMVAHMGTKEAWLLALSTFERIHRKDLWERGQGEDLVRVTPSNGLCSLESQSTCLSDVSPDAPRKDPQITYKDYVRRKFRLMEDRNARLGECVNLSHRYTRLLLVKEHSNPIWAQQKLLETGWEHSRTRGHQASPIQMETLFEPDEERPEPPRTVVLQGAAGMGKSMLTHKVMLDWADGRLFQDQFDYVFYISCRELNRSHTQCSVHDLLSSCWPEHGAPLEDLIRAPDRLLFIIDGFHELHPSFHDVQGPWCHCWEEKRPTELLLGSLIRRLLLPQLSLLITTRPCALEKLHGLLEHPRHVEILGFSEAEREEYFYRYFHNTGQASQVFSFMRDYEPLFTMCFVPMVSWVVCTCLKQQLESGELLRQTSRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFEEEDLGKHGLDGAEVSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFTAMYCALHGREAVRRALAEYGFSERNFLAHTVRFLFGLLNEEMRCYLERNLGWTISPQVKEEALAWIQNKARSEGSTLQHGSLELLSCLYEIQEEDFIQQALSHFQVVVVRNLSTKMEHVVCSFCARYCRGTEVLHLYGSAYSTGAEDGPPEPPGAQTQSTHSQERNILPDIYSAYLSATICTNSNLIELALYRNALGSQGVRLLCQGLRHANCKLQNLRLKRCHISGSACQDLAAAIIANRNLIRLDLSGNSIGVLGLELLCEGLQHPMCRLQMIQLKICHLGQASCEDLASTLKMNQSLMELDLGLNDLGDSGALLLCEGLRHPDCKLQTLRLGICRLGSDACAGVASVLQVNTCLRELDLSFNDLGDRGLWLLGEGLRHQTCRLQKLWLDSCGLTSKACEDLSSVLGISQTLNELYLTNNALGDTGVRLLCKRLRHPGCKLRVLWLFGMDLNKVTHRRMAALRVTKPYLDIGC

Target ORF information:

RefSeq Version	XM_017588881.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588881.1

ORF Insert Sequence:

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCAGCTGAAG 
ATCTGCCACC TTGGACAAGC TTCCTGTGAA GATCTGGCTT CTACTCTCAA AATGAACCAG 
AGCCTGATGG AGCTGGACCT GGGTCTGAAT GACCTTGGGG ATTCTGGGGC GCTTCTGCTG 
TGTGAGGGCC TCAGGCATCC AGATTGCAAA CTCCAGACCC TCCGGCTGGG CATTTGCCGA 
CTGGGCTCAG ACGCGTGTGC AGGGGTTGCT AGTGTGCTCC AGGTCAACAC GTGCCTCCGA 
GAGCTGGACC TGAGCTTCAA TGACTTGGGA GACAGGGGCC TGTGGCTGCT GGGGGAAGGA 
CTGAGGCACC AGACCTGCAG ACTCCAGAAG CTCTGGCTGG ACAGTTGCGG CCTCACTTCC 
AAAGCATGTG AGGACCTGTC TTCAGTCCTG GGAATCAGTC AGACCCTGAA TGAGCTTTAT 
CTGACCAATA ATGCTCTGGG GGACACTGGT GTTCGTCTGC TGTGCAAGAG GCTGAGGCAT 
CCGGGGTGCA AGCTTCGTGT CCTGTGGCTT TTCGGAATGG ACCTGAACAA AGTGACCCAC 
AGGAGGATGG CGGCACTTCG AGTCACAAAA CCATACCTAG ATATTGGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp12 ( XM_017588882.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444371.1 Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa56432
Clone ID Related Accession (Same CDS sequence)	XM_017588882.1
Accession Version	XM_017588882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTATG CTCTCTTGAG AGCCAGTCAA CGTGCCTTTC GGACGTCTCT CCTGATGCTC 
CAAGAAAAGA CCTTTCCTTC CTCTCTACTA GATCCCCAGA TAACCTACAA AGACTATGTC 
CGAAGGAAAT TCCGGCTAAT GGAAGACCGC AATGCGCGAC TAGGCGAATG TGTGAACTTA 
AGTCATCGTT ACACTCGGCT TCTCCTAGTA AAGGAACACT CAAATCCTAT CTGGGCACAG 
CAAAAACTTC TGGAAACAGG GTGGGAACAC TCCAGAACCA GGGGCCACCA GGCTAGCCCT 
ATCCAAATGG AGACCCTCTT TGAGCCAGAT GAAGAACGCC CGGAGCCACC ACGCACAGTG 
GTATTACAAG GGGCAGCGGG GATGGGGAAG TCCATGTTGA CCCACAAAGT GATGTTGGAC 
TGGGCCGATG GGAGACTCTT CCAAGACCAG TTCGATTATG TCTTCTATAT CAGCTGCAGG 
GAGTTGAATA GAAGCCACAC CCAGTGCAGT GTACATGACC TCCTCTCCAG CTGCTGGCCA 
GAGCATGGTG CACCCCTCGA GGACCTCATC CGGGCTCCTG ATCGTCTTCT ATTCATCATC 
GATGGCTTCC ATGAGCTTCA TCCCTCTTTC CATGACGTTC AGGGTCCCTG GTGCCACTGC 
TGGGAGGAGA AACGACCCAC GGAACTCCTC CTCGGAAGCC TGATTCGGAG GTTGCTTCTG 
CCCCAGCTCT CTTTGCTCAT CACCACACGA CCCTGTGCAC TGGAGAAGCT GCACGGCTTG 
TTGGAACACC CTAGGCACGT GGAGATCCTG GGCTTCTCCG AGGCAGAGAG GGAGGAATAT 
TTCTACAGAT ATTTCCACAA CACTGGACAA GCGAGCCAAG TGTTCAGCTT CATGAGGGAC 
TATGAGCCCC TCTTTACCAT GTGTTTTGTT CCCATGGTGT CCTGGGTGGT CTGCACCTGC 
TTAAAGCAGC AGCTAGAAAG TGGGGAGCTT TTAAGACAAA CATCTAGGAC CACCACAGCG 
GTTTATATGT TCTACCTTCT GAGCCTGATG CAGCCCAAGC CAGGGACCCC AACCTTCAAA 
GTCCCAGCCA ACCAGAGAGG CCTGGTCTCT CTGGCTGCAG AGGGCCTCTG GAATCAGAAG 
ATTCTCTTCG AGGAAGAGGA TCTTGGGAAA CATGGCCTAG ATGGAGCAGA GGTCTCCACC 
TTCCTCAACG TGAACATATT CCAGAAGGGC ATCAAGTGTG AGAAATTCTA CAGCTTCATC 
CACCTGAGTT TCCAGGAATT CTTCACAGCC ATGTACTGTG CACTGCACGG CAGAGAGGCG 
GTGAGGAGAG CACTGGCTGA GTATGGTTTC TCAGAAAGGA ACTTCTTGGC CCACACCGTC 
CGCTTTCTCT TTGGCCTCCT CAATGAAGAG ATGAGATGCT ACCTCGAGAG GAATCTCGGC 
TGGACCATCT CCCCTCAGGT CAAGGAGGAA GCATTGGCGT GGATCCAAAA CAAGGCTCGA 
AGTGAGGGCT CCACCCTGCA GCATGGCTCC CTGGAGCTTC TCAGCTGCTT GTACGAGATC 
CAGGAGGAAG ACTTCATCCA GCAGGCCCTG AGCCACTTTC AGGTGGTTGT TGTCAGAAAC 
CTCTCAACGA AGATGGAGCA CGTGGTCTGC TCGTTTTGTG CGAGGTACTG CAGAGGCACA 
GAAGTGCTTC ACTTGTATGG GAGTGCCTAT AGTACAGGCG CAGAGGATGG CCCGCCAGAA 
CCTCCAGGAG CCCAGACGCA GTCCACACAC TCACAGGAAA GGAACATCCT TCCTGACATT 
TACAGTGCAT ACCTCTCAGC AACCATCTGC ACCAACTCAA ACCTGATCGA GCTGGCCTTG 
TATCGAAACG CCTTGGGCAG CCAGGGCGTG AGGCTGCTCT GTCAAGGCCT CCGACATGCA 
AACTGCAAGC TTCAGAACCT GAGGCTGAAG AGGTGTCACA TCTCCGGATC AGCCTGCCAG 
GATCTCGCAG CGGCTATCAT CGCCAATAGG AATTTAATCA GGCTGGACCT CAGTGGCAAC 
AGCATTGGAG TGCTAGGCCT AGAGCTGCTC TGTGAGGGGT TACAGCACCC CATGTGTAGG 
CTGCAGATGA TCCATTTTTC AAGTCCCTGC TCCTCCCGAG AACTGTGGAT GAAGAATAGA 
ACTTGGCCTA CTCTTGACCC AAGGCTGAGG AAGTGTTTGC TGGAGGCTGC GGCTGGCCGA 
GCACTGGCCT CTGTTCTCAG CAACAACTCA CATCTTGTAG AACTGGATCT GACAGGAAAC 
CCCTTGGAGG ATTTGGGGCT GAAGTTACTG TGTCAAGGGC TGAGGCACCC AGTCTGCAGG 
CTGCGGACCT TGTGGCTGAA GATCTGCCAC CTTGGACAAG CTTCCTGTGA AGATCTGGCT 
TCTACTCTCA AAATGAACCA GAGCCTGATG GAGCTGGACC TGGGTCTGAA TGACCTTGGG 
GATTCTGGGG CGCTTCTGCT GTGTGAGGGC CTCAGGCATC CAGATTGCAA ACTCCAGACC 
CTCCGGCTGG GCATTTGCCG ACTGGGCTCA GACGCGTGTG CAGGGGTTGC TAGTGTGCTC 
CAGGTCAACA CGTGCCTCCG AGAGCTGGAC CTGAGCTTCA ATGACTTGGG AGACAGGGGC 
CTGTGGCTGC TGGGGGAAGG ACTGAGGCAC CAGACCTGCA GACTCCAGAA GCTCTGGCTG 
GACAGTTGCG GCCTCACTTC CAAAGCATGT GAGGACCTGT CTTCAGTCCT GGGAATCAGT 
CAGACCCTGA ATGAGCTTTA TCTGACCAAT AATGCTCTGG GGGACACTGG TGTTCGTCTG 
CTGTGCAAGA GGCTGAGGCA TCCGGGGTGC AAGCTTCGTG TCCTGTGGCT TTTCGGAATG 
GACCTGAACA AAGTGACCCA CAGGAGGATG GCGGCACTTC GAGTCACAAA ACCATACCTA 
GATATTGGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444371.1
CDS	99..3053
Translation	MVYALLRASQRAFRTSLLMLQEKTFPSSLLDPQITYKDYVRRKFRLMEDRNARLGECVNLSHRYTRLLLVKEHSNPIWAQQKLLETGWEHSRTRGHQASPIQMETLFEPDEERPEPPRTVVLQGAAGMGKSMLTHKVMLDWADGRLFQDQFDYVFYISCRELNRSHTQCSVHDLLSSCWPEHGAPLEDLIRAPDRLLFIIDGFHELHPSFHDVQGPWCHCWEEKRPTELLLGSLIRRLLLPQLSLLITTRPCALEKLHGLLEHPRHVEILGFSEAEREEYFYRYFHNTGQASQVFSFMRDYEPLFTMCFVPMVSWVVCTCLKQQLESGELLRQTSRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFEEEDLGKHGLDGAEVSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFTAMYCALHGREAVRRALAEYGFSERNFLAHTVRFLFGLLNEEMRCYLERNLGWTISPQVKEEALAWIQNKARSEGSTLQHGSLELLSCLYEIQEEDFIQQALSHFQVVVVRNLSTKMEHVVCSFCARYCRGTEVLHLYGSAYSTGAEDGPPEPPGAQTQSTHSQERNILPDIYSAYLSATICTNSNLIELALYRNALGSQGVRLLCQGLRHANCKLQNLRLKRCHISGSACQDLAAAIIANRNLIRLDLSGNSIGVLGLELLCEGLQHPMCRLQMIHFSSPCSSRELWMKNRTWPTLDPRLRKCLLEAAAGRALASVLSNNSHLVELDLTGNPLEDLGLKLLCQGLRHPVCRLRTLWLKICHLGQASCEDLASTLKMNQSLMELDLGLNDLGDSGALLLCEGLRHPDCKLQTLRLGICRLGSDACAGVASVLQVNTCLRELDLSFNDLGDRGLWLLGEGLRHQTCRLQKLWLDSCGLTSKACEDLSSVLGISQTLNELYLTNNALGDTGVRLLCKRLRHPGCKLRVLWLFGMDLNKVTHRRMAALRVTKPYLDIGC

Target ORF information:

RefSeq Version	XM_017588882.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588882.1

ORF Insert Sequence:

ATGGTCTATG CTCTCTTGAG AGCCAGTCAA CGTGCCTTTC GGACGTCTCT CCTGATGCTC 
CAAGAAAAGA CCTTTCCTTC CTCTCTACTA GATCCCCAGA TAACCTACAA AGACTATGTC 
CGAAGGAAAT TCCGGCTAAT GGAAGACCGC AATGCGCGAC TAGGCGAATG TGTGAACTTA 
AGTCATCGTT ACACTCGGCT TCTCCTAGTA AAGGAACACT CAAATCCTAT CTGGGCACAG 
CAAAAACTTC TGGAAACAGG GTGGGAACAC TCCAGAACCA GGGGCCACCA GGCTAGCCCT 
ATCCAAATGG AGACCCTCTT TGAGCCAGAT GAAGAACGCC CGGAGCCACC ACGCACAGTG 
GTATTACAAG GGGCAGCGGG GATGGGGAAG TCCATGTTGA CCCACAAAGT GATGTTGGAC 
TGGGCCGATG GGAGACTCTT CCAAGACCAG TTCGATTATG TCTTCTATAT CAGCTGCAGG 
GAGTTGAATA GAAGCCACAC CCAGTGCAGT GTACATGACC TCCTCTCCAG CTGCTGGCCA 
GAGCATGGTG CACCCCTCGA GGACCTCATC CGGGCTCCTG ATCGTCTTCT ATTCATCATC 
GATGGCTTCC ATGAGCTTCA TCCCTCTTTC CATGACGTTC AGGGTCCCTG GTGCCACTGC 
TGGGAGGAGA AACGACCCAC GGAACTCCTC CTCGGAAGCC TGATTCGGAG GTTGCTTCTG 
CCCCAGCTCT CTTTGCTCAT CACCACACGA CCCTGTGCAC TGGAGAAGCT GCACGGCTTG 
TTGGAACACC CTAGGCACGT GGAGATCCTG GGCTTCTCCG AGGCAGAGAG GGAGGAATAT 
TTCTACAGAT ATTTCCACAA CACTGGACAA GCGAGCCAAG TGTTCAGCTT CATGAGGGAC 
TATGAGCCCC TCTTTACCAT GTGTTTTGTT CCCATGGTGT CCTGGGTGGT CTGCACCTGC 
TTAAAGCAGC AGCTAGAAAG TGGGGAGCTT TTAAGACAAA CATCTAGGAC CACCACAGCG 
GTTTATATGT TCTACCTTCT GAGCCTGATG CAGCCCAAGC CAGGGACCCC AACCTTCAAA 
GTCCCAGCCA ACCAGAGAGG CCTGGTCTCT CTGGCTGCAG AGGGCCTCTG GAATCAGAAG 
ATTCTCTTCG AGGAAGAGGA TCTTGGGAAA CATGGCCTAG ATGGAGCAGA GGTCTCCACC 
TTCCTCAACG TGAACATATT CCAGAAGGGC ATCAAGTGTG AGAAATTCTA CAGCTTCATC 
CACCTGAGTT TCCAGGAATT CTTCACAGCC ATGTACTGTG CACTGCACGG CAGAGAGGCG 
GTGAGGAGAG CACTGGCTGA GTATGGTTTC TCAGAAAGGA ACTTCTTGGC CCACACCGTC 
CGCTTTCTCT TTGGCCTCCT CAATGAAGAG ATGAGATGCT ACCTCGAGAG GAATCTCGGC 
TGGACCATCT CCCCTCAGGT CAAGGAGGAA GCATTGGCGT GGATCCAAAA CAAGGCTCGA 
AGTGAGGGCT CCACCCTGCA GCATGGCTCC CTGGAGCTTC TCAGCTGCTT GTACGAGATC 
CAGGAGGAAG ACTTCATCCA GCAGGCCCTG AGCCACTTTC AGGTGGTTGT TGTCAGAAAC 
CTCTCAACGA AGATGGAGCA CGTGGTCTGC TCGTTTTGTG CGAGGTACTG CAGAGGCACA 
GAAGTGCTTC ACTTGTATGG GAGTGCCTAT AGTACAGGCG CAGAGGATGG CCCGCCAGAA 
CCTCCAGGAG CCCAGACGCA GTCCACACAC TCACAGGAAA GGAACATCCT TCCTGACATT 
TACAGTGCAT ACCTCTCAGC AACCATCTGC ACCAACTCAA ACCTGATCGA GCTGGCCTTG 
TATCGAAACG CCTTGGGCAG CCAGGGCGTG AGGCTGCTCT GTCAAGGCCT CCGACATGCA 
AACTGCAAGC TTCAGAACCT GAGGCTGAAG AGGTGTCACA TCTCCGGATC AGCCTGCCAG 
GATCTCGCAG CGGCTATCAT CGCCAATAGG AATTTAATCA GGCTGGACCT CAGTGGCAAC 
AGCATTGGAG TGCTAGGCCT AGAGCTGCTC TGTGAGGGGT TACAGCACCC CATGTGTAGG 
CTGCAGATGA TCCATTTTTC AAGTCCCTGC TCCTCCCGAG AACTGTGGAT GAAGAATAGA 
ACTTGGCCTA CTCTTGACCC AAGGCTGAGG AAGTGTTTGC TGGAGGCTGC GGCTGGCCGA 
GCACTGGCCT CTGTTCTCAG CAACAACTCA CATCTTGTAG AACTGGATCT GACAGGAAAC 
CCCTTGGAGG ATTTGGGGCT GAAGTTACTG TGTCAAGGGC TGAGGCACCC AGTCTGCAGG 
CTGCGGACCT TGTGGCTGAA GATCTGCCAC CTTGGACAAG CTTCCTGTGA AGATCTGGCT 
TCTACTCTCA AAATGAACCA GAGCCTGATG GAGCTGGACC TGGGTCTGAA TGACCTTGGG 
GATTCTGGGG CGCTTCTGCT GTGTGAGGGC CTCAGGCATC CAGATTGCAA ACTCCAGACC 
CTCCGGCTGG GCATTTGCCG ACTGGGCTCA GACGCGTGTG CAGGGGTTGC TAGTGTGCTC 
CAGGTCAACA CGTGCCTCCG AGAGCTGGAC CTGAGCTTCA ATGACTTGGG AGACAGGGGC 
CTGTGGCTGC TGGGGGAAGG ACTGAGGCAC CAGACCTGCA GACTCCAGAA GCTCTGGCTG 
GACAGTTGCG GCCTCACTTC CAAAGCATGT GAGGACCTGT CTTCAGTCCT GGGAATCAGT 
CAGACCCTGA ATGAGCTTTA TCTGACCAAT AATGCTCTGG GGGACACTGG TGTTCGTCTG 
CTGTGCAAGA GGCTGAGGCA TCCGGGGTGC AAGCTTCGTG TCCTGTGGCT TTTCGGAATG 
GACCTGAACA AAGTGACCCA CAGGAGGATG GCGGCACTTC GAGTCACAAA ACCATACCTA 
GATATTGGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp12 ( XM_017588883.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444372.1 Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa56433
Clone ID Related Accession (Same CDS sequence)	XM_017588883.1
Accession Version	XM_017588883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CGCTGAGGAA GTGTTTGCTG GAGGCTGCGG CTGGCCGAGC ACTGGCCTCT 
GTTCTCAGCA ACAACTCACA TCTTGTAGAA CTGGATCTGA CAGGAAACCC CTTGGAGGAT 
TTGGGGCTGA AGTTACTGTG TCAAGGGCTG AGGCACCCAG TCTGCAGGCT GCGGACCTTG 
TGGCTGAAGA TCTGCCACCT TGGACAAGCT TCCTGTGAAG ATCTGGCTTC TACTCTCAAA 
ATGAACCAGA GCCTGATGGA GCTGGACCTG GGTCTGAATG ACCTTGGGGA TTCTGGGGCG 
CTTCTGCTGT GTGAGGGCCT CAGGCATCCA GATTGCAAAC TCCAGACCCT CCGGCTGGGC 
ATTTGCCGAC TGGGCTCAGA CGCGTGTGCA GGGGTTGCTA GTGTGCTCCA GGTCAACACG 
TGCCTCCGAG AGCTGGACCT GAGCTTCAAT GACTTGGGAG ACAGGGGCCT GTGGCTGCTG 
GGGGAAGGAC TGAGGCACCA GACCTGCAGA CTCCAGAAGC TCTGGCTGGA CAGTTGCGGC 
CTCACTTCCA AAGCATGTGA GGACCTGTCT TCAGTCCTGG GAATCAGTCA GACCCTGAAT 
GAGCTTTATC TGACCAATAA TGCTCTGGGG GACACTGGTG TTCGTCTGCT GTGCAAGAGG 
CTGAGGCATC CGGGGTGCAA GCTTCGTGTC CTGTGGCTTT TCGGAATGGA CCTGAACAAA 
GTGACCCACA GGAGGATGGC GGCACTTCGA GTCACAAAAC CATACCTAGA TATTGGGTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444372.1
CDS	199..3021
Translation	MLPATAKDALHRLSTYLEELEAGELKKFKLYLGIAEELGQDKIPRGRMEMAGPLEMAQLMVAHMGTKEAWLLALSTFERIHRKDLWERGQGEDLVRVTPSNGLCSLESQSTCLSDVSPDAPRKDPQITYKDYVRRKFRLMEDRNARLGECVNLSHRYTRLLLVKEHSNPIWAQQKLLETGWEHSRTRGHQASPIQMETLFEPDEERPEPPRTVVLQGAAGMGKSMLTHKVMLDWADGRLFQDQFDYVFYISCRELNRSHTQCSVHDLLSSCWPEHGAPLEDLIRAPDRLLFIIDGFHELHPSFHDVQGPWCHCWEEKRPTELLLGSLIRRLLLPQLSLLITTRPCALEKLHGLLEHPRHVEILGFSEAEREEYFYRYFHNTGQASQVFSFMRDYEPLFTMCFVPMVSWVVCTCLKQQLESGELLRQTSRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFEEEDLGKHGLDGAEVSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFTAMYCALHGREAVRRALAEYGFSERNFLAHTVRFLFGLLNEEMRCYLERNLGWTISPQVKEEALAWIQNKARSEGSTLQHGSLELLSCLYEIQEEDFIQQALSHFQVVVVRNLSTKMEHVVCSFCARYCRGTEVLHLYGSAYSTGAEDGPPEPPGAQTQSTHSLRKCLLEAAAGRALASVLSNNSHLVELDLTGNPLEDLGLKLLCQGLRHPVCRLRTLWLKICHLGQASCEDLASTLKMNQSLMELDLGLNDLGDSGALLLCEGLRHPDCKLQTLRLGICRLGSDACAGVASVLQVNTCLRELDLSFNDLGDRGLWLLGEGLRHQTCRLQKLWLDSCGLTSKACEDLSSVLGISQTLNELYLTNNALGDTGVRLLCKRLRHPGCKLRVLWLFGMDLNKVTHRRMAALRVTKPYLDIGC

Target ORF information:

RefSeq Version	XM_017588883.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588883.1

ORF Insert Sequence:

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CGCTGAGGAA GTGTTTGCTG GAGGCTGCGG CTGGCCGAGC ACTGGCCTCT 
GTTCTCAGCA ACAACTCACA TCTTGTAGAA CTGGATCTGA CAGGAAACCC CTTGGAGGAT 
TTGGGGCTGA AGTTACTGTG TCAAGGGCTG AGGCACCCAG TCTGCAGGCT GCGGACCTTG 
TGGCTGAAGA TCTGCCACCT TGGACAAGCT TCCTGTGAAG ATCTGGCTTC TACTCTCAAA 
ATGAACCAGA GCCTGATGGA GCTGGACCTG GGTCTGAATG ACCTTGGGGA TTCTGGGGCG 
CTTCTGCTGT GTGAGGGCCT CAGGCATCCA GATTGCAAAC TCCAGACCCT CCGGCTGGGC 
ATTTGCCGAC TGGGCTCAGA CGCGTGTGCA GGGGTTGCTA GTGTGCTCCA GGTCAACACG 
TGCCTCCGAG AGCTGGACCT GAGCTTCAAT GACTTGGGAG ACAGGGGCCT GTGGCTGCTG 
GGGGAAGGAC TGAGGCACCA GACCTGCAGA CTCCAGAAGC TCTGGCTGGA CAGTTGCGGC 
CTCACTTCCA AAGCATGTGA GGACCTGTCT TCAGTCCTGG GAATCAGTCA GACCCTGAAT 
GAGCTTTATC TGACCAATAA TGCTCTGGGG GACACTGGTG TTCGTCTGCT GTGCAAGAGG 
CTGAGGCATC CGGGGTGCAA GCTTCGTGTC CTGTGGCTTT TCGGAATGGA CCTGAACAAA 
GTGACCCACA GGAGGATGGC GGCACTTCGA GTCACAAAAC CATACCTAGA TATTGGGTGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp12 ( XM_017588879.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444368.1 Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56430
Clone ID Related Accession (Same CDS sequence)	XM_017588879.1 , XM_017588880.1
Accession Version	XM_017588879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCATTTTTCA 
AGTCCCTGCT CCTCCCGAGA ACTGTGGATG AAGAATAGAA CTTGGCCTAC TCTTGACCCA 
AGGCTGAGGA AGTGTTTGCT GGAGGCTGCG GCTGGCCGAG CACTGGCCTC TGTTCTCAGC 
AACAACTCAC ATCTTGTAGA ACTGGATCTG ACAGGAAACC CCTTGGAGGA TTTGGGGCTG 
AAGTTACTGT GTCAAGGGCT GAGGCACCCA GTCTGCAGGC TGCGGACCTT GTGGCTGAAG 
ATCTGCCACC TTGGACAAGC TTCCTGTGAA GATCTGGCTT CTACTCTCAA AATGAACCAG 
AGCCTGATGG AGCTGGACCT GGGTCTGAAT GACCTTGGGG ATTCTGGGGC GCTTCTGCTG 
TGTGAGGGCC TCAGGCATCC AGATTGCAAA CTCCAGACCC TCCGGCTGGG CATTTGCCGA 
CTGGGCTCAG ACGCGTGTGC AGGGGTTGCT AGTGTGCTCC AGGTCAACAC GTGCCTCCGA 
GAGCTGGACC TGAGCTTCAA TGACTTGGGA GACAGGGGCC TGTGGCTGCT GGGGGAAGGA 
CTGAGGCACC AGACCTGCAG ACTCCAGAAG CTCTGGCTGG ACAGTTGCGG CCTCACTTCC 
AAAGCATGTG AGGACCTGTC TTCAGTCCTG GGAATCAGTC AGACCCTGAA TGAGCTTTAT 
CTGACCAATA ATGCTCTGGG GGACACTGGT GTTCGTCTGC TGTGCAAGAG GCTGAGGCAT 
CCGGGGTGCA AGCTTCGTGT CCTGTGGCTT TTCGGAATGG ACCTGAACAA AGTGACCCAC 
AGGAGGATGG CGGCACTTCG AGTCACAAAA CCATACCTAG ATATTGGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444368.1
CDS	201..3434
Translation	MLPATAKDALHRLSTYLEELEAGELKKFKLYLGIAEELGQDKIPRGRMEMAGPLEMAQLMVAHMGTKEAWLLALSTFERIHRKDLWERGQGEDLVRVTPSNGLCSLESQSTCLSDVSPDAPRKDPQITYKDYVRRKFRLMEDRNARLGECVNLSHRYTRLLLVKEHSNPIWAQQKLLETGWEHSRTRGHQASPIQMETLFEPDEERPEPPRTVVLQGAAGMGKSMLTHKVMLDWADGRLFQDQFDYVFYISCRELNRSHTQCSVHDLLSSCWPEHGAPLEDLIRAPDRLLFIIDGFHELHPSFHDVQGPWCHCWEEKRPTELLLGSLIRRLLLPQLSLLITTRPCALEKLHGLLEHPRHVEILGFSEAEREEYFYRYFHNTGQASQVFSFMRDYEPLFTMCFVPMVSWVVCTCLKQQLESGELLRQTSRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFEEEDLGKHGLDGAEVSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFTAMYCALHGREAVRRALAEYGFSERNFLAHTVRFLFGLLNEEMRCYLERNLGWTISPQVKEEALAWIQNKARSEGSTLQHGSLELLSCLYEIQEEDFIQQALSHFQVVVVRNLSTKMEHVVCSFCARYCRGTEVLHLYGSAYSTGAEDGPPEPPGAQTQSTHSQERNILPDIYSAYLSATICTNSNLIELALYRNALGSQGVRLLCQGLRHANCKLQNLRLKRCHISGSACQDLAAAIIANRNLIRLDLSGNSIGVLGLELLCEGLQHPMCRLQMIHFSSPCSSRELWMKNRTWPTLDPRLRKCLLEAAAGRALASVLSNNSHLVELDLTGNPLEDLGLKLLCQGLRHPVCRLRTLWLKICHLGQASCEDLASTLKMNQSLMELDLGLNDLGDSGALLLCEGLRHPDCKLQTLRLGICRLGSDACAGVASVLQVNTCLRELDLSFNDLGDRGLWLLGEGLRHQTCRLQKLWLDSCGLTSKACEDLSSVLGISQTLNELYLTNNALGDTGVRLLCKRLRHPGCKLRVLWLFGMDLNKVTHRRMAALRVTKPYLDIGC

Target ORF information:

RefSeq Version	XM_017588879.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588879.1

ORF Insert Sequence:

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCATTTTTCA 
AGTCCCTGCT CCTCCCGAGA ACTGTGGATG AAGAATAGAA CTTGGCCTAC TCTTGACCCA 
AGGCTGAGGA AGTGTTTGCT GGAGGCTGCG GCTGGCCGAG CACTGGCCTC TGTTCTCAGC 
AACAACTCAC ATCTTGTAGA ACTGGATCTG ACAGGAAACC CCTTGGAGGA TTTGGGGCTG 
AAGTTACTGT GTCAAGGGCT GAGGCACCCA GTCTGCAGGC TGCGGACCTT GTGGCTGAAG 
ATCTGCCACC TTGGACAAGC TTCCTGTGAA GATCTGGCTT CTACTCTCAA AATGAACCAG 
AGCCTGATGG AGCTGGACCT GGGTCTGAAT GACCTTGGGG ATTCTGGGGC GCTTCTGCTG 
TGTGAGGGCC TCAGGCATCC AGATTGCAAA CTCCAGACCC TCCGGCTGGG CATTTGCCGA 
CTGGGCTCAG ACGCGTGTGC AGGGGTTGCT AGTGTGCTCC AGGTCAACAC GTGCCTCCGA 
GAGCTGGACC TGAGCTTCAA TGACTTGGGA GACAGGGGCC TGTGGCTGCT GGGGGAAGGA 
CTGAGGCACC AGACCTGCAG ACTCCAGAAG CTCTGGCTGG ACAGTTGCGG CCTCACTTCC 
AAAGCATGTG AGGACCTGTC TTCAGTCCTG GGAATCAGTC AGACCCTGAA TGAGCTTTAT 
CTGACCAATA ATGCTCTGGG GGACACTGGT GTTCGTCTGC TGTGCAAGAG GCTGAGGCAT 
CCGGGGTGCA AGCTTCGTGT CCTGTGGCTT TTCGGAATGG ACCTGAACAA AGTGACCCAC 
AGGAGGATGG CGGCACTTCG AGTCACAAAA CCATACCTAG ATATTGGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp12 ( XM_017588880.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444369.1 Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X2, mRNA.

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CloneID	ORa56430
Clone ID Related Accession (Same CDS sequence)	XM_017588879.1 , XM_017588880.1
Accession Version	XM_017588880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCATTTTTCA 
AGTCCCTGCT CCTCCCGAGA ACTGTGGATG AAGAATAGAA CTTGGCCTAC TCTTGACCCA 
AGGCTGAGGA AGTGTTTGCT GGAGGCTGCG GCTGGCCGAG CACTGGCCTC TGTTCTCAGC 
AACAACTCAC ATCTTGTAGA ACTGGATCTG ACAGGAAACC CCTTGGAGGA TTTGGGGCTG 
AAGTTACTGT GTCAAGGGCT GAGGCACCCA GTCTGCAGGC TGCGGACCTT GTGGCTGAAG 
ATCTGCCACC TTGGACAAGC TTCCTGTGAA GATCTGGCTT CTACTCTCAA AATGAACCAG 
AGCCTGATGG AGCTGGACCT GGGTCTGAAT GACCTTGGGG ATTCTGGGGC GCTTCTGCTG 
TGTGAGGGCC TCAGGCATCC AGATTGCAAA CTCCAGACCC TCCGGCTGGG CATTTGCCGA 
CTGGGCTCAG ACGCGTGTGC AGGGGTTGCT AGTGTGCTCC AGGTCAACAC GTGCCTCCGA 
GAGCTGGACC TGAGCTTCAA TGACTTGGGA GACAGGGGCC TGTGGCTGCT GGGGGAAGGA 
CTGAGGCACC AGACCTGCAG ACTCCAGAAG CTCTGGCTGG ACAGTTGCGG CCTCACTTCC 
AAAGCATGTG AGGACCTGTC TTCAGTCCTG GGAATCAGTC AGACCCTGAA TGAGCTTTAT 
CTGACCAATA ATGCTCTGGG GGACACTGGT GTTCGTCTGC TGTGCAAGAG GCTGAGGCAT 
CCGGGGTGCA AGCTTCGTGT CCTGTGGCTT TTCGGAATGG ACCTGAACAA AGTGACCCAC 
AGGAGGATGG CGGCACTTCG AGTCACAAAA CCATACCTAG ATATTGGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444369.1
CDS	201..3434
Translation	MLPATAKDALHRLSTYLEELEAGELKKFKLYLGIAEELGQDKIPRGRMEMAGPLEMAQLMVAHMGTKEAWLLALSTFERIHRKDLWERGQGEDLVRVTPSNGLCSLESQSTCLSDVSPDAPRKDPQITYKDYVRRKFRLMEDRNARLGECVNLSHRYTRLLLVKEHSNPIWAQQKLLETGWEHSRTRGHQASPIQMETLFEPDEERPEPPRTVVLQGAAGMGKSMLTHKVMLDWADGRLFQDQFDYVFYISCRELNRSHTQCSVHDLLSSCWPEHGAPLEDLIRAPDRLLFIIDGFHELHPSFHDVQGPWCHCWEEKRPTELLLGSLIRRLLLPQLSLLITTRPCALEKLHGLLEHPRHVEILGFSEAEREEYFYRYFHNTGQASQVFSFMRDYEPLFTMCFVPMVSWVVCTCLKQQLESGELLRQTSRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFEEEDLGKHGLDGAEVSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFTAMYCALHGREAVRRALAEYGFSERNFLAHTVRFLFGLLNEEMRCYLERNLGWTISPQVKEEALAWIQNKARSEGSTLQHGSLELLSCLYEIQEEDFIQQALSHFQVVVVRNLSTKMEHVVCSFCARYCRGTEVLHLYGSAYSTGAEDGPPEPPGAQTQSTHSQERNILPDIYSAYLSATICTNSNLIELALYRNALGSQGVRLLCQGLRHANCKLQNLRLKRCHISGSACQDLAAAIIANRNLIRLDLSGNSIGVLGLELLCEGLQHPMCRLQMIHFSSPCSSRELWMKNRTWPTLDPRLRKCLLEAAAGRALASVLSNNSHLVELDLTGNPLEDLGLKLLCQGLRHPVCRLRTLWLKICHLGQASCEDLASTLKMNQSLMELDLGLNDLGDSGALLLCEGLRHPDCKLQTLRLGICRLGSDACAGVASVLQVNTCLRELDLSFNDLGDRGLWLLGEGLRHQTCRLQKLWLDSCGLTSKACEDLSSVLGISQTLNELYLTNNALGDTGVRLLCKRLRHPGCKLRVLWLFGMDLNKVTHRRMAALRVTKPYLDIGC

Target ORF information:

RefSeq Version	XM_017588880.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588880.1

ORF Insert Sequence:

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCATTTTTCA 
AGTCCCTGCT CCTCCCGAGA ACTGTGGATG AAGAATAGAA CTTGGCCTAC TCTTGACCCA 
AGGCTGAGGA AGTGTTTGCT GGAGGCTGCG GCTGGCCGAG CACTGGCCTC TGTTCTCAGC 
AACAACTCAC ATCTTGTAGA ACTGGATCTG ACAGGAAACC CCTTGGAGGA TTTGGGGCTG 
AAGTTACTGT GTCAAGGGCT GAGGCACCCA GTCTGCAGGC TGCGGACCTT GTGGCTGAAG 
ATCTGCCACC TTGGACAAGC TTCCTGTGAA GATCTGGCTT CTACTCTCAA AATGAACCAG 
AGCCTGATGG AGCTGGACCT GGGTCTGAAT GACCTTGGGG ATTCTGGGGC GCTTCTGCTG 
TGTGAGGGCC TCAGGCATCC AGATTGCAAA CTCCAGACCC TCCGGCTGGG CATTTGCCGA 
CTGGGCTCAG ACGCGTGTGC AGGGGTTGCT AGTGTGCTCC AGGTCAACAC GTGCCTCCGA 
GAGCTGGACC TGAGCTTCAA TGACTTGGGA GACAGGGGCC TGTGGCTGCT GGGGGAAGGA 
CTGAGGCACC AGACCTGCAG ACTCCAGAAG CTCTGGCTGG ACAGTTGCGG CCTCACTTCC 
AAAGCATGTG AGGACCTGTC TTCAGTCCTG GGAATCAGTC AGACCCTGAA TGAGCTTTAT 
CTGACCAATA ATGCTCTGGG GGACACTGGT GTTCGTCTGC TGTGCAAGAG GCTGAGGCAT 
CCGGGGTGCA AGCTTCGTGT CCTGTGGCTT TTCGGAATGG ACCTGAACAA AGTGACCCAC 
AGGAGGATGG CGGCACTTCG AGTCACAAAA CCATACCTAG ATATTGGGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp12 ( NM_001169142.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001162613.1 Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), mRNA.

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CloneID	ORa30178
Clone ID Related Accession (Same CDS sequence)	XM_008758753.2 , NM_001169142.1
Accession Version	NM_001169142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC106193.5. On or before Dec 18, 2009 this sequence version replaced XM_001066862.1, XM_218181.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689594 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCAGCTGAGG 
AAGTGTTTGC TGGAGGCTGC GGCTGGCCGA GCACTGGCCT CTGTTCTCAG CAACAACTCA 
CATCTTGTAG AACTGGATCT GACAGGAAAC CCCTTGGAGG ATTTGGGGCT GAAGTTACTG 
TGTCAAGGGC TGAGGCACCC AGTCTGCAGG CTGCGGACCT TGTGGCTGAA GATCTGCCAC 
CTTGGACAAG CTTCCTGTGA AGATCTGGCT TCTACTCTCA AAATGAACCA GAGCCTGATG 
GAGCTGGACC TGGGTCTGAA TGACCTTGGG GATTCTGGGG CGCTTCTGCT GTGTGAGGGC 
CTCAGGCATC CAGATTGCAA ACTCCAGACC CTCCGGCTGG GCATTTGCCG ACTGGGCTCA 
GACGCGTGTG CAGGGGTTGC TAGTGTGCTC CAGGTCAACA CGTGCCTCCG AGAGCTGGAC 
CTGAGCTTCA ATGACTTGGG AGACAGGGGC CTGTGGCTGC TGGGGGAAGG ACTGAGGCAC 
CAGACCTGCA GACTCCAGAA GCTCTGGCTG GACAGTTGCG GCCTCACTTC CAAAGCATGT 
GAGGACCTGT CTTCAGTCCT GGGAATCAGT CAGACCCTGA ATGAGCTTTA TCTGACCAAT 
AATGCTCTGG GGGACACTGG TGTTCGTCTG CTGTGCAAGA GGCTGAGGCA TCCGGGGTGC 
AAGCTTCGTG TCCTGTGGCT TTTCGGAATG GACCTGAACA AAGTGACCCA CAGGAGGATG 
GCGGCACTTC GAGTCACAAA ACCATACCTA GATATTGGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162613.1
CDS	1..3165
Translation	MLPATAKDALHRLSTYLEELEAGELKKFKLYLGIAEELGQDKIPRGRMEMAGPLEMAQLMVAHMGTKEAWLLALSTFERIHRKDLWERGQGEDLVRVTPSNGLCSLESQSTCLSDVSPDAPRKDPQITYKDYVRRKFRLMEDRNARLGECVNLSHRYTRLLLVKEHSNPIWAQQKLLETGWEHSRTRGHQASPIQMETLFEPDEERPEPPRTVVLQGAAGMGKSMLTHKVMLDWADGRLFQDQFDYVFYISCRELNRSHTQCSVHDLLSSCWPEHGAPLEDLIRAPDRLLFIIDGFHELHPSFHDVQGPWCHCWEEKRPTELLLGSLIRRLLLPQLSLLITTRPCALEKLHGLLEHPRHVEILGFSEAEREEYFYRYFHNTGQASQVFSFMRDYEPLFTMCFVPMVSWVVCTCLKQQLESGELLRQTSRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFEEEDLGKHGLDGAEVSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFTAMYCALHGREAVRRALAEYGFSERNFLAHTVRFLFGLLNEEMRCYLERNLGWTISPQVKEEALAWIQNKARSEGSTLQHGSLELLSCLYEIQEEDFIQQALSHFQVVVVRNLSTKMEHVVCSFCARYCRGTEVLHLYGSAYSTGAEDGPPEPPGAQTQSTHSQERNILPDIYSAYLSATICTNSNLIELALYRNALGSQGVRLLCQGLRHANCKLQNLRLKRCHISGSACQDLAAAIIANRNLIRLDLSGNSIGVLGLELLCEGLQHPMCRLQMIQLRKCLLEAAAGRALASVLSNNSHLVELDLTGNPLEDLGLKLLCQGLRHPVCRLRTLWLKICHLGQASCEDLASTLKMNQSLMELDLGLNDLGDSGALLLCEGLRHPDCKLQTLRLGICRLGSDACAGVASVLQVNTCLRELDLSFNDLGDRGLWLLGEGLRHQTCRLQKLWLDSCGLTSKACEDLSSVLGISQTLNELYLTNNALGDTGVRLLCKRLRHPGCKLRVLWLFGMDLNKVTHRRMAALRVTKPYLDIGC

Target ORF information:

RefSeq Version	NM_001169142.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169142.1

ORF Insert Sequence:

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCAGCTGAGG 
AAGTGTTTGC TGGAGGCTGC GGCTGGCCGA GCACTGGCCT CTGTTCTCAG CAACAACTCA 
CATCTTGTAG AACTGGATCT GACAGGAAAC CCCTTGGAGG ATTTGGGGCT GAAGTTACTG 
TGTCAAGGGC TGAGGCACCC AGTCTGCAGG CTGCGGACCT TGTGGCTGAA GATCTGCCAC 
CTTGGACAAG CTTCCTGTGA AGATCTGGCT TCTACTCTCA AAATGAACCA GAGCCTGATG 
GAGCTGGACC TGGGTCTGAA TGACCTTGGG GATTCTGGGG CGCTTCTGCT GTGTGAGGGC 
CTCAGGCATC CAGATTGCAA ACTCCAGACC CTCCGGCTGG GCATTTGCCG ACTGGGCTCA 
GACGCGTGTG CAGGGGTTGC TAGTGTGCTC CAGGTCAACA CGTGCCTCCG AGAGCTGGAC 
CTGAGCTTCA ATGACTTGGG AGACAGGGGC CTGTGGCTGC TGGGGGAAGG ACTGAGGCAC 
CAGACCTGCA GACTCCAGAA GCTCTGGCTG GACAGTTGCG GCCTCACTTC CAAAGCATGT 
GAGGACCTGT CTTCAGTCCT GGGAATCAGT CAGACCCTGA ATGAGCTTTA TCTGACCAAT 
AATGCTCTGG GGGACACTGG TGTTCGTCTG CTGTGCAAGA GGCTGAGGCA TCCGGGGTGC 
AAGCTTCGTG TCCTGTGGCT TTTCGGAATG GACCTGAACA AAGTGACCCA CAGGAGGATG 
GCGGCACTTC GAGTCACAAA ACCATACCTA GATATTGGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nlrp12 ( XM_008758753.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008756975.1 Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X3, mRNA.

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CloneID	ORa30178
Clone ID Related Accession (Same CDS sequence)	XM_008758753.2 , NM_001169142.1
Accession Version	XM_008758753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008758753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCAGCTGAGG 
AAGTGTTTGC TGGAGGCTGC GGCTGGCCGA GCACTGGCCT CTGTTCTCAG CAACAACTCA 
CATCTTGTAG AACTGGATCT GACAGGAAAC CCCTTGGAGG ATTTGGGGCT GAAGTTACTG 
TGTCAAGGGC TGAGGCACCC AGTCTGCAGG CTGCGGACCT TGTGGCTGAA GATCTGCCAC 
CTTGGACAAG CTTCCTGTGA AGATCTGGCT TCTACTCTCA AAATGAACCA GAGCCTGATG 
GAGCTGGACC TGGGTCTGAA TGACCTTGGG GATTCTGGGG CGCTTCTGCT GTGTGAGGGC 
CTCAGGCATC CAGATTGCAA ACTCCAGACC CTCCGGCTGG GCATTTGCCG ACTGGGCTCA 
GACGCGTGTG CAGGGGTTGC TAGTGTGCTC CAGGTCAACA CGTGCCTCCG AGAGCTGGAC 
CTGAGCTTCA ATGACTTGGG AGACAGGGGC CTGTGGCTGC TGGGGGAAGG ACTGAGGCAC 
CAGACCTGCA GACTCCAGAA GCTCTGGCTG GACAGTTGCG GCCTCACTTC CAAAGCATGT 
GAGGACCTGT CTTCAGTCCT GGGAATCAGT CAGACCCTGA ATGAGCTTTA TCTGACCAAT 
AATGCTCTGG GGGACACTGG TGTTCGTCTG CTGTGCAAGA GGCTGAGGCA TCCGGGGTGC 
AAGCTTCGTG TCCTGTGGCT TTTCGGAATG GACCTGAACA AAGTGACCCA CAGGAGGATG 
GCGGCACTTC GAGTCACAAA ACCATACCTA GATATTGGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008756975.1
CDS	201..3365
Translation	MLPATAKDALHRLSTYLEELEAGELKKFKLYLGIAEELGQDKIPRGRMEMAGPLEMAQLMVAHMGTKEAWLLALSTFERIHRKDLWERGQGEDLVRVTPSNGLCSLESQSTCLSDVSPDAPRKDPQITYKDYVRRKFRLMEDRNARLGECVNLSHRYTRLLLVKEHSNPIWAQQKLLETGWEHSRTRGHQASPIQMETLFEPDEERPEPPRTVVLQGAAGMGKSMLTHKVMLDWADGRLFQDQFDYVFYISCRELNRSHTQCSVHDLLSSCWPEHGAPLEDLIRAPDRLLFIIDGFHELHPSFHDVQGPWCHCWEEKRPTELLLGSLIRRLLLPQLSLLITTRPCALEKLHGLLEHPRHVEILGFSEAEREEYFYRYFHNTGQASQVFSFMRDYEPLFTMCFVPMVSWVVCTCLKQQLESGELLRQTSRTTTAVYMFYLLSLMQPKPGTPTFKVPANQRGLVSLAAEGLWNQKILFEEEDLGKHGLDGAEVSTFLNVNIFQKGIKCEKFYSFIHLSFQEFFTAMYCALHGREAVRRALAEYGFSERNFLAHTVRFLFGLLNEEMRCYLERNLGWTISPQVKEEALAWIQNKARSEGSTLQHGSLELLSCLYEIQEEDFIQQALSHFQVVVVRNLSTKMEHVVCSFCARYCRGTEVLHLYGSAYSTGAEDGPPEPPGAQTQSTHSQERNILPDIYSAYLSATICTNSNLIELALYRNALGSQGVRLLCQGLRHANCKLQNLRLKRCHISGSACQDLAAAIIANRNLIRLDLSGNSIGVLGLELLCEGLQHPMCRLQMIQLRKCLLEAAAGRALASVLSNNSHLVELDLTGNPLEDLGLKLLCQGLRHPVCRLRTLWLKICHLGQASCEDLASTLKMNQSLMELDLGLNDLGDSGALLLCEGLRHPDCKLQTLRLGICRLGSDACAGVASVLQVNTCLRELDLSFNDLGDRGLWLLGEGLRHQTCRLQKLWLDSCGLTSKACEDLSSVLGISQTLNELYLTNNALGDTGVRLLCKRLRHPGCKLRVLWLFGMDLNKVTHRRMAALRVTKPYLDIGC

Target ORF information:

RefSeq Version	XM_008758753.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NLR family, pyrin domain containing 12 (Nlrp12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008758753.2

ORF Insert Sequence:

ATGTTGCCAG CTACAGCCAA GGATGCCCTC CATCGACTGT CCACCTACCT GGAAGAACTT 
GAGGCTGGGG AACTGAAGAA ATTCAAATTA TACCTGGGGA TTGCGGAGGA ACTGGGCCAG 
GACAAGATCC CCAGGGGACG AATGGAGATG GCTGGTCCTC TGGAAATGGC CCAATTGATG 
GTGGCCCATA TGGGGACAAA GGAGGCTTGG CTTCTGGCTC TCAGCACCTT TGAGAGGATC 
CACAGGAAGG ACCTGTGGGA GCGAGGACAG GGAGAAGACC TGGTGAGGGT GACTCCAAGT 
AATGGTCTAT GCTCTCTTGA GAGCCAGTCA ACGTGCCTTT CGGACGTCTC TCCTGATGCT 
CCAAGAAAAG ATCCCCAGAT AACCTACAAA GACTATGTCC GAAGGAAATT CCGGCTAATG 
GAAGACCGCA ATGCGCGACT AGGCGAATGT GTGAACTTAA GTCATCGTTA CACTCGGCTT 
CTCCTAGTAA AGGAACACTC AAATCCTATC TGGGCACAGC AAAAACTTCT GGAAACAGGG 
TGGGAACACT CCAGAACCAG GGGCCACCAG GCTAGCCCTA TCCAAATGGA GACCCTCTTT 
GAGCCAGATG AAGAACGCCC GGAGCCACCA CGCACAGTGG TATTACAAGG GGCAGCGGGG 
ATGGGGAAGT CCATGTTGAC CCACAAAGTG ATGTTGGACT GGGCCGATGG GAGACTCTTC 
CAAGACCAGT TCGATTATGT CTTCTATATC AGCTGCAGGG AGTTGAATAG AAGCCACACC 
CAGTGCAGTG TACATGACCT CCTCTCCAGC TGCTGGCCAG AGCATGGTGC ACCCCTCGAG 
GACCTCATCC GGGCTCCTGA TCGTCTTCTA TTCATCATCG ATGGCTTCCA TGAGCTTCAT 
CCCTCTTTCC ATGACGTTCA GGGTCCCTGG TGCCACTGCT GGGAGGAGAA ACGACCCACG 
GAACTCCTCC TCGGAAGCCT GATTCGGAGG TTGCTTCTGC CCCAGCTCTC TTTGCTCATC 
ACCACACGAC CCTGTGCACT GGAGAAGCTG CACGGCTTGT TGGAACACCC TAGGCACGTG 
GAGATCCTGG GCTTCTCCGA GGCAGAGAGG GAGGAATATT TCTACAGATA TTTCCACAAC 
ACTGGACAAG CGAGCCAAGT GTTCAGCTTC ATGAGGGACT ATGAGCCCCT CTTTACCATG 
TGTTTTGTTC CCATGGTGTC CTGGGTGGTC TGCACCTGCT TAAAGCAGCA GCTAGAAAGT 
GGGGAGCTTT TAAGACAAAC ATCTAGGACC ACCACAGCGG TTTATATGTT CTACCTTCTG 
AGCCTGATGC AGCCCAAGCC AGGGACCCCA ACCTTCAAAG TCCCAGCCAA CCAGAGAGGC 
CTGGTCTCTC TGGCTGCAGA GGGCCTCTGG AATCAGAAGA TTCTCTTCGA GGAAGAGGAT 
CTTGGGAAAC ATGGCCTAGA TGGAGCAGAG GTCTCCACCT TCCTCAACGT GAACATATTC 
CAGAAGGGCA TCAAGTGTGA GAAATTCTAC AGCTTCATCC ACCTGAGTTT CCAGGAATTC 
TTCACAGCCA TGTACTGTGC ACTGCACGGC AGAGAGGCGG TGAGGAGAGC ACTGGCTGAG 
TATGGTTTCT CAGAAAGGAA CTTCTTGGCC CACACCGTCC GCTTTCTCTT TGGCCTCCTC 
AATGAAGAGA TGAGATGCTA CCTCGAGAGG AATCTCGGCT GGACCATCTC CCCTCAGGTC 
AAGGAGGAAG CATTGGCGTG GATCCAAAAC AAGGCTCGAA GTGAGGGCTC CACCCTGCAG 
CATGGCTCCC TGGAGCTTCT CAGCTGCTTG TACGAGATCC AGGAGGAAGA CTTCATCCAG 
CAGGCCCTGA GCCACTTTCA GGTGGTTGTT GTCAGAAACC TCTCAACGAA GATGGAGCAC 
GTGGTCTGCT CGTTTTGTGC GAGGTACTGC AGAGGCACAG AAGTGCTTCA CTTGTATGGG 
AGTGCCTATA GTACAGGCGC AGAGGATGGC CCGCCAGAAC CTCCAGGAGC CCAGACGCAG 
TCCACACACT CACAGGAAAG GAACATCCTT CCTGACATTT ACAGTGCATA CCTCTCAGCA 
ACCATCTGCA CCAACTCAAA CCTGATCGAG CTGGCCTTGT ATCGAAACGC CTTGGGCAGC 
CAGGGCGTGA GGCTGCTCTG TCAAGGCCTC CGACATGCAA ACTGCAAGCT TCAGAACCTG 
AGGCTGAAGA GGTGTCACAT CTCCGGATCA GCCTGCCAGG ATCTCGCAGC GGCTATCATC 
GCCAATAGGA ATTTAATCAG GCTGGACCTC AGTGGCAACA GCATTGGAGT GCTAGGCCTA 
GAGCTGCTCT GTGAGGGGTT ACAGCACCCC ATGTGTAGGC TGCAGATGAT CCAGCTGAGG 
AAGTGTTTGC TGGAGGCTGC GGCTGGCCGA GCACTGGCCT CTGTTCTCAG CAACAACTCA 
CATCTTGTAG AACTGGATCT GACAGGAAAC CCCTTGGAGG ATTTGGGGCT GAAGTTACTG 
TGTCAAGGGC TGAGGCACCC AGTCTGCAGG CTGCGGACCT TGTGGCTGAA GATCTGCCAC 
CTTGGACAAG CTTCCTGTGA AGATCTGGCT TCTACTCTCA AAATGAACCA GAGCCTGATG 
GAGCTGGACC TGGGTCTGAA TGACCTTGGG GATTCTGGGG CGCTTCTGCT GTGTGAGGGC 
CTCAGGCATC CAGATTGCAA ACTCCAGACC CTCCGGCTGG GCATTTGCCG ACTGGGCTCA 
GACGCGTGTG CAGGGGTTGC TAGTGTGCTC CAGGTCAACA CGTGCCTCCG AGAGCTGGAC 
CTGAGCTTCA ATGACTTGGG AGACAGGGGC CTGTGGCTGC TGGGGGAAGG ACTGAGGCAC 
CAGACCTGCA GACTCCAGAA GCTCTGGCTG GACAGTTGCG GCCTCACTTC CAAAGCATGT 
GAGGACCTGT CTTCAGTCCT GGGAATCAGT CAGACCCTGA ATGAGCTTTA TCTGACCAAT 
AATGCTCTGG GGGACACTGG TGTTCGTCTG CTGTGCAAGA GGCTGAGGCA TCCGGGGTGC 
AAGCTTCGTG TCCTGTGGCT TTTCGGAATG GACCTGAACA AAGTGACCCA CAGGAGGATG 
GCGGCACTTC GAGTCACAAA ACCATACCTA GATATTGGGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NLRP12 suppresses colon inflammation and tumorigenesis through the negative regulation of noncanonical NF-?B signaling.
Immunity36(5)742-54(2012 May)
Allen IC,Wilson JE,Schneider M,Lich JD,Roberts RA,Arthur JC,Woodford RM,Davis BK,Uronis JM,Herfarth HH,Jobin C,Rogers AB,Ting JP

Cutting edge: NLRP12 controls dendritic and myeloid cell migration to affect contact hypersensitivity.
Journal of immunology (Baltimore, Md. : 1950)185(8)4515-9(2010 Oct)
Arthur JC,Lich JD,Ye Z,Allen IC,Gris D,Wilson JE,Schneider M,Roney KE,O'Connor BP,Moore CB,Morrison A,Sutterwala FS,Bertin J,Koller BH,Liu Z,Ting JP

Mutations in NALP12 cause hereditary periodic fever syndromes.
Proceedings of the National Academy of Sciences of the United States of America105(5)1614-9(2008 Feb)
J?ru I,Duquesnoy P,Fernandes-Alnemri T,Cochet E,Yu JW,Lackmy-Port-Lis M,Grimprel E,Landman-Parker J,Hentgen V,Marlin S,McElreavey K,Sarkisian T,Grateau G,Alnemri ES,Amselem S

Monarch-1 suppresses non-canonical NF-kappaB activation and p52-dependent chemokine expression in monocytes.
Journal of immunology (Baltimore, Md. : 1950)178(3)1256-60(2007 Feb)
Lich JD,Williams KL,Moore CB,Arthur JC,Davis BK,Taxman DJ,Ting JP

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Nlrp12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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