Tnni3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnni3
Entrez Gene ID	29248
Full Name	troponin I3, cardiac type
Synonyms	TnI,cTNI
General protein information	Preferred Names troponin I3, cardiac type Names troponin I, cardiac muscle cardiac troponin I troponin 1, type 3 troponin I type 3 (cardiac)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12
Summary	cardiac isoform of troponin I, which is a subunit of the troponin complex; inhibits actomyosin ATPase activity in muscle conraction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017144.2	NP_058840.1	troponin I, cardiac muscle

Pathways from BioSystems

Homologies

	TNNI3	XP_001085820.1
Mus musculus (house mouse)	Tnni3	NP_033432.1
Rattus norvegicus (Norway rat)	Tnni3	NP_058840.1
Pan troglodytes (chimpanzee)	TNNI3	XP_001134934.2
Bos taurus (cattle)	LOC511094	NP_001035607.1
Xenopus tropicalis (tropical clawed frog)	tnni3	NP_001011410.1
Homo sapiens (human)	TNNI3	NP_000354.4
Canis lupus familiaris (dog)	TNNI3	NP_001003041.1

Gene Ontology
Bibliography

Related articles in PubMed

The concerted movement of the switch region of Troponin I in cardiac muscle thin filaments as tracked by conventional and pulsed (DEER) EPR.
Potluri PR, Chamoun J, Cooke JA, Badr M, Guse JA, Rayes R, Cordina NM, McCamey D, Fajer PG, Brown LJ
Journal of structural biology200(3)376-387(2017 Dec)

Functional significance of C-terminal mobile domain of cardiac troponin I.
Bohlooli Ghashghaee N, Tanner BCW, Dong WJ
Archives of biochemistry and biophysics63438-46(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Compared to cTnI(WT), both truncations displayed greater Ca(2+)-sensitivity and faster cross-bridge attachment rates at both SLs. Furthermore, cTnI(1-167) slowed MgADP release rate and enhanced cross-bridge binding. Our findings imply that cTnI-MD truncations affect the blocked-to closed-state transition(s) and destabilize the closed-state position of tropomyosin.
Title: Functional significance of C-terminal mobile domain of cardiac troponin I.

PKC phosphorylates cardiac troponin I (cTnI) S23/24, S43/45 and T144 to fine tune myocyte function.
Title: Functional communication between PKC-targeted cardiac troponin I phosphorylation sites.

These findings demonstrate that S-nitrosylation and S-glutathionylation exert opposing effects on Ca(2+) sensitivity in mammalian FT muscle fibers, mediated by competitive actions on Cys134 of TnIf.

cTnIS43/45N is a functionally conservative substitution, and may be appropriate for use as a phospho-null in rodent models designed for studies on PKC modulation of cardiac performance
Title: Functionally conservative substitutions at cardiac troponin I S43/45.

the interaction between cTnC and cTnI in skinned papillary muscle strips is dependent on sarcomere length
Title: Sarcomere length dependent effects on the interaction between cTnC and cTnI in skinned papillary muscle strips.

The following Tnni3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnni3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13315	NM_017144.2 Latest version!	Rattus norvegicus troponin I3, cardiac type (Tnni3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnni3 ( NM_017144.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058840.1 Rattus norvegicus troponin I3, cardiac type (Tnni3), mRNA.

Price & Availability↑

CloneID	ORa13315
Clone ID Related Accession (Same CDS sequence)	NM_017144.2
Accession Version	NM_017144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, cardiac muscle
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X58499.1. On Aug 31, 2012 this sequence version replaced NM_017144.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X58499.1, CK358716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATG AGAGCAGCGA TGCGGCTGGG GAACCCCAGC CGGCGCCTGC TCCTGTCCGA 
CGTCGCTCCT CGGCCAACTA CCGAGCCTAT GCCACCGAGC CACATGCCAA GAAAAAGTCT 
AAGATCTCCG CCTCCAGAAA ACTTCAGTTG AAGACTCTGA TGCTGCAGAT TGCGAAGCAG 
GAGATGGAGC GTGAGGCAGA GGAGCGACGT GGAGAGAAGG GCCGCGTTCT GAGCACGCGT 
TGCCAGCCCT TGGTGTTGGA TGGGCTGGGC TTTGAAGAGC TTCAGGACCT ATGCCGGCAG 
CTTCACGCTC GTGTGGACAA AGTGGATGAA GAGAGATACG ACGTGGAAGC AAAAGTCACC 
AAGAACATCA CTGAGATTGC AGATCTGACC CAGAAGATCT ATGACCTGCG TGGCAAGTTT 
AAGCGGCCCA CTCTCCGAAG AGTGAGAATC TCAGCAGATG CCATGATGCA GGCACTACTG 
GGGACCCGGG CCAAGGAATC CTTGGACCTG AGGGCCCACC TCAAGCAGGT GAAGAAGGAG 
GACATTGAGA AGGAAAACCG GGAGGTGGGA GACTGGCGCA AGAATATCGA TGCACTAAGT 
GGAATGGAAG GCCGAAAGAA AAAGTTTGAG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058840.1
CDS	105..740
Translation	MADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQEMEREAEERRGEKGRVLSTRCQPLVLDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDIEKENREVGDWRKNIDALSGMEGRKKKFEG

Target ORF information:

RefSeq Version	NM_017144.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I3, cardiac type (Tnni3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017144.2

ORF Insert Sequence:

ATGGCGGATG AGAGCAGCGA TGCGGCTGGG GAACCCCAGC CGGCGCCTGC TCCTGTCCGA 
CGTCGCTCCT CGGCCAACTA CCGAGCCTAT GCCACCGAGC CACATGCCAA GAAAAAGTCT 
AAGATCTCCG CCTCCAGAAA ACTTCAGTTG AAGACTCTGA TGCTGCAGAT TGCGAAGCAG 
GAGATGGAGC GTGAGGCAGA GGAGCGACGT GGAGAGAAGG GCCGCGTTCT GAGCACGCGT 
TGCCAGCCCT TGGTGTTGGA TGGGCTGGGC TTTGAAGAGC TTCAGGACCT ATGCCGGCAG 
CTTCACGCTC GTGTGGACAA AGTGGATGAA GAGAGATACG ACGTGGAAGC AAAAGTCACC 
AAGAACATCA CTGAGATTGC AGATCTGACC CAGAAGATCT ATGACCTGCG TGGCAAGTTT 
AAGCGGCCCA CTCTCCGAAG AGTGAGAATC TCAGCAGATG CCATGATGCA GGCACTACTG 
GGGACCCGGG CCAAGGAATC CTTGGACCTG AGGGCCCACC TCAAGCAGGT GAAGAAGGAG 
GACATTGAGA AGGAAAACCG GGAGGTGGGA GACTGGCGCA AGAATATCGA TGCACTAAGT 
GGAATGGAAG GCCGAAAGAA AAAGTTTGAG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The concerted movement of the switch region of Troponin I in cardiac muscle thin filaments as tracked by conventional and pulsed (DEER) EPR.
Journal of structural biology200(3)376-387(2017 Dec)
Potluri PR,Chamoun J,Cooke JA,Badr M,Guse JA,Rayes R,Cordina NM,McCamey D,Fajer PG,Brown LJ

Functional significance of C-terminal mobile domain of cardiac troponin I.
Archives of biochemistry and biophysics63438-46(2017 Nov)
Bohlooli Ghashghaee N,Tanner BCW,Dong WJ

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Tnni3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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