Polb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polb
Entrez Gene ID	29240
Full Name	DNA polymerase beta
General protein information	Preferred Names DNA polymerase beta Names DNA polymerase beta DNA pol beta DNA-directed DNA polymerase beta polymerase (DNA directed), beta polymerase (DNA) beta polymerase-beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5
Summary	plays a role in DNA repair and bypass of pyrimidine dimers produced by UV-irradiation; also involved in base-excision repair [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017141.2	NP_058837.2	DNA polymerase beta

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Polb	NP_035260.1
Rattus norvegicus (Norway rat)	Polb	NP_058837.2
Xenopus tropicalis (tropical clawed frog)	polb	NP_001006894.2
Homo sapiens (human)	POLB	NP_002681.1
	POLB	XP_001097548.1
Canis lupus familiaris (dog)	POLB	XP_532790.2
Bos taurus (cattle)	POLB	NP_001029936.1
Gallus gallus (chicken)	POLB	XP_424407.2
Danio rerio (zebrafish)	polb	NP_001003879.1
Pan troglodytes (chimpanzee)	POLB	XP_001143904.1

Gene Ontology
Bibliography

Related articles in PubMed

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wilson R, Geyer SH, Reissig L, Rose J, Szumska D, Hardman E, Prin F, McGuire C, Ramirez-Solis R, White J, Galli A, Tudor C, Tuck E, Mazzeo CI, Smith JC, Robertson E, Adams DJ, Mohun T, Weninger WJ
Wellcome open research11(2016)

Characterization of the redox transition of the XRCC1 N-terminal domain.
Gabel SA, Smith CE, Cuneo MJ, Mueller GA, Kirby TW, DeRose EF, Krahn JM, London RE
Structure (London, England : 1993)22(12)1754-63(2014 Dec)

Opposing roles of mitochondrial and nuclear PARP1 in the regulation of mitochondrial and nuclear DNA integrity: implications for the regulation of mitochondrial function.
Szczesny B, Brunyanszki A, Olah G, Mitra S, Szabo C
Nucleic acids research42(21)13161-73(2014 Dec)

Mutation of POLB causes lupus in mice.
Senejani AG, Liu Y, Kidane D, Maher SE, Zeiss CJ, Park HJ, Kashgarian M, McNiff JM, Zelterman D, Bothwell AL, Sweasy JB
Cell reports6(1)1-8(2014 Jan)

Structural changes in the hydrophobic hinge region adversely affect the activity and fidelity of the I260Q mutator DNA polymerase ?.
Gridley CL, Rangarajan S, Firbank S, Dalal S, Sweasy JB, Jaeger J
Biochemistry52(25)4422-32(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The I260Q variant of DNA polymerase beta is an efficient mutator polymerase with fairly indiscriminate misincorporation activities opposite all template bases.
Title: Structural changes in the hydrophobic hinge region adversely affect the activity and fidelity of the I260Q mutator DNA polymerase ?.

The closed state of E295K has a more distorted active site than the active site in the wild-type Polb.
Title: Unfavorable electrostatic and steric interactions in DNA polymerase ? E295K mutant interfere with the enzyme's pathway.

decline in pol beta activity started between 30 and 45 days postnatal in all the tissues. Post mitotic tissues showed pronounced decline than the proliferating tissues
Title: Age-related decline in DNA polymerase ? activity in rat brain and tissues.

at low pH the chemical step is rate limiting for catalysis, but at high pH, a postchemistry conformational step is rate limiting due to a pH-dependent increase in the rate of nucleotidyl transfer
Title: Kinetic mechanism of active site assembly and chemical catalysis of DNA polymerase ?.

These results point to the hinge region of DNA polymerase beta as being critical in the maintenance of the proper geometry of the dNTP binding pocket.
Title: Hinge residue I174 is critical for proper dNTP selection by DNA polymerase beta.

The following Polb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02999	NM_017141.2 Latest version!	Rattus norvegicus DNA polymerase beta (Polb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polb ( NM_017141.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058837.2 Rattus norvegicus DNA polymerase beta (Polb), mRNA.

Price & Availability↑

CloneID	ORa02999
Clone ID Related Accession (Same CDS sequence)	NM_017141.2
Accession Version	NM_017141.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098668.1. On Jun 10, 2007 this sequence version replaced NM_017141.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098668.1, U38801.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAAAC GCAAGGCCCC GCAGGAGACC CTCAACGGCG GCATCACGGA CATGCTCGTG 
GAACTCGCAA ACTTTGAGAA GAACGTGAGC CAGGCGATCC ACAAGTACAA TGCATACAGA 
AAAGCAGCAT CTGTGATAGC AAAGTACCCA CACAAAATCA AGAGTGGAGC AGAAGCTAAG 
AAATTGCCAG GAGTAGGAAC AAAAATTGCT GAAAAGATTG ATGAATTTTT AGCAACTGGA 
AAATTGCGTA AACTGGAAAA GATTCGTCAG GATGATACAA GTTCATCCAT CAACTTCCTG 
ACTCGAGTTA CTGGCATCGG GCCATCTGCT GCAAGGAAGC TTGTAGATGA AGGAATTAAA 
ACATTAGAAG ATCTCAGGAA AAATGAAGAT AAATTGAACC ACCATCAGCG AATTGGGCTG 
AAATATTTTG AGGACTTTGA AAAGAGAATT CCTCGTGAGG AGATGCTGCA AATGCAGGAC 
ATTGTTCTTA ATGAAGTTAA AAAGCTGGAT CCCGAGTACA TCGCTACAGT CTGCGGCAGT 
TTCCGAAGAG GCGCAGAGTC CAGTGGAGAT ATGGACGTTC TGCTGACCCA CCCAAACTTC 
ACGTCAGAAT CAAGCAAACA GCCAAAGTTG TTACATCGTG TTGTGGAACA GTTACAAAAA 
GTCCGTTTCA TTACAGATAC TCTGTCAAAG GGTGAGACAA AGTTCATGGG TGTTTGCCAG 
CTTCCCAGCG AGAATGATGA AAACGAATAT CCACACAGGA GAATCGATAT CAGGTTGATC 
CCCAAAGATC AGTACTACTG TGGTGTTCTC TACTTCACTG GAAGTGACAT CTTTAATAAG 
AATATGAGAG CGCATGCCCT GGAAAAGGGC TTCACAATCA ATGAGTACAC CATCCGCCCC 
CTGGGGGTCA CTGGGGTTGC TGGGGAGCCC CTTCCGGTGG ACAGCGAGCA GGACATTTTC 
GATTACATCC AGTGGCGCTA CCGGGAGCCC AAGGACAGGA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058837.2
CDS	13..1020
Translation	MSKRKAPQETLNGGITDMLVELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVTGIGPSAARKLVDEGIKTLEDLRKNEDKLNHHQRIGLKYFEDFEKRIPREEMLQMQDIVLNEVKKLDPEYIATVCGSFRRGAESSGDMDVLLTHPNFTSESSKQPKLLHRVVEQLQKVRFITDTLSKGETKFMGVCQLPSENDENEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEQDIFDYIQWRYREPKDRSE

Target ORF information:

RefSeq Version	NM_017141.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA polymerase beta (Polb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017141.2

ORF Insert Sequence:

ATGAGCAAAC GCAAGGCCCC GCAGGAGACC CTCAACGGCG GCATCACGGA CATGCTCGTG 
GAACTCGCAA ACTTTGAGAA GAACGTGAGC CAGGCGATCC ACAAGTACAA TGCATACAGA 
AAAGCAGCAT CTGTGATAGC AAAGTACCCA CACAAAATCA AGAGTGGAGC AGAAGCTAAG 
AAATTGCCAG GAGTAGGAAC AAAAATTGCT GAAAAGATTG ATGAATTTTT AGCAACTGGA 
AAATTGCGTA AACTGGAAAA GATTCGTCAG GATGATACAA GTTCATCCAT CAACTTCCTG 
ACTCGAGTTA CTGGCATCGG GCCATCTGCT GCAAGGAAGC TTGTAGATGA AGGAATTAAA 
ACATTAGAAG ATCTCAGGAA AAATGAAGAT AAATTGAACC ACCATCAGCG AATTGGGCTG 
AAATATTTTG AGGACTTTGA AAAGAGAATT CCTCGTGAGG AGATGCTGCA AATGCAGGAC 
ATTGTTCTTA ATGAAGTTAA AAAGCTGGAT CCCGAGTACA TCGCTACAGT CTGCGGCAGT 
TTCCGAAGAG GCGCAGAGTC CAGTGGAGAT ATGGACGTTC TGCTGACCCA CCCAAACTTC 
ACGTCAGAAT CAAGCAAACA GCCAAAGTTG TTACATCGTG TTGTGGAACA GTTACAAAAA 
GTCCGTTTCA TTACAGATAC TCTGTCAAAG GGTGAGACAA AGTTCATGGG TGTTTGCCAG 
CTTCCCAGCG AGAATGATGA AAACGAATAT CCACACAGGA GAATCGATAT CAGGTTGATC 
CCCAAAGATC AGTACTACTG TGGTGTTCTC TACTTCACTG GAAGTGACAT CTTTAATAAG 
AATATGAGAG CGCATGCCCT GGAAAAGGGC TTCACAATCA ATGAGTACAC CATCCGCCCC 
CTGGGGGTCA CTGGGGTTGC TGGGGAGCCC CTTCCGGTGG ACAGCGAGCA GGACATTTTC 
GATTACATCC AGTGGCGCTA CCGGGAGCCC AAGGACAGGA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wellcome open research11(2016)
Wilson R,Geyer SH,Reissig L,Rose J,Szumska D,Hardman E,Prin F,McGuire C,Ramirez-Solis R,White J,Galli A,Tudor C,Tuck E,Mazzeo CI,Smith JC,Robertson E,Adams DJ,Mohun T,Weninger WJ

Characterization of the redox transition of the XRCC1 N-terminal domain.
Structure (London, England : 1993)22(12)1754-63(2014 Dec)
Gabel SA,Smith CE,Cuneo MJ,Mueller GA,Kirby TW,DeRose EF,Krahn JM,London RE

Opposing roles of mitochondrial and nuclear PARP1 in the regulation of mitochondrial and nuclear DNA integrity: implications for the regulation of mitochondrial function.
Nucleic acids research42(21)13161-73(2014 Dec)
Szczesny B,Brunyanszki A,Olah G,Mitra S,Szabo C

Mutation of POLB causes lupus in mice.
Cell reports6(1)1-8(2014 Jan)
Senejani AG,Liu Y,Kidane D,Maher SE,Zeiss CJ,Park HJ,Kashgarian M,McNiff JM,Zelterman D,Bothwell AL,Sweasy JB

Structural changes in the hydrophobic hinge region adversely affect the activity and fidelity of the I260Q mutator DNA polymerase ?.
Biochemistry52(25)4422-32(2013 Jun)
Gridley CL,Rangarajan S,Firbank S,Dalal S,Sweasy JB,Jaeger J

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Polb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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