Eif3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif3a
Entrez Gene ID	292148
Full Name	eukaryotic translation initiation factor 3, subunit A
Synonyms	Eif3s10,ZH12
General protein information	Preferred Names eukaryotic translation initiation factor 3, subunit A Names eukaryotic translation initiation factor 3 subunit A eIF-3-theta eukaryotic translation initiation factor 3 subunit 10 eukaryotic translation initiation factor 3, subunit 10 (theta)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q55

mRNA and Protein(s)

mRNA	Protein	Name
NM_001047087.2	NP_001040552.2	eukaryotic translation initiation factor 3 subunit A

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF3A	NP_003741.1
Pan troglodytes (chimpanzee)	EIF3A	XP_001153575.1
Mus musculus (house mouse)	Eif3a	NP_034253.3
Oryza sativa (rice)	Os01g0120800	NP_001041871.1
Danio rerio (zebrafish)	eif3s10	NP_956114.2
Anopheles gambiae (African malaria mosquito)	EIF3A_ANOGA	XP_312628.5
Caenorhabditis elegans (roundworm)	egl-45	NP_498698.1
Saccharomyces cerevisiae (baker's yeast)	RPG1	NP_009635.1
Arabidopsis thaliana (thale cress)	EIF3A	NP_192881.1
Bos taurus (cattle)	EIF3A	NP_001193243.1
Drosophila melanogaster (fruit fly)	eIF3-S10	NP_649470.2
Kluyveromyces lactis	KLLA0C14498g	XP_452849.1
Neurospora crassa	NCU00040	XP_956151.2
Rattus norvegicus (Norway rat)	Eif3a	NP_001040552.2
Gallus gallus (chicken)	EIF3A	XP_421787.3
Schizosaccharomyces pombe (fission yeast)	tif32	NP_596379.1
Magnaporthe oryzae (rice blast fungus)	MGG_10192	XP_003713822.1
Canis lupus familiaris (dog)	EIF3A	XP_852131.1
Eremothecium gossypii	AGOS_ABL065W	NP_982882.1
Xenopus tropicalis (tropical clawed frog)	eif3a	NP_001096173.1

Gene Ontology
Bibliography

Related articles in PubMed

The Role of miR-497/EIF3A Axis in TGF?1-Induced Epithelial-Mesenchymal Transition and Extracellular Matrix in Rat Alveolar Epithelial Cells and Pulmonary Fibroblasts.
Guo R, Lv Y, Ouyang Y, Liu S, Li D
Journal of cellular biochemistry118(10)3401-3408(2017 Oct)

eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation.
Lee AS, Kranzusch PJ, Doudna JA, Cate JH
Nature536(7614)96-9(2016 08)

Knockdown of eIF3a ameliorates cardiac fibrosis by inhibiting the TGF-?1/Smad3 signaling pathway.
Li B, Chen H, Yang X, Wang Y, Qin L, Chu Y
Cellular and molecular biology (Noisy-le-Grand, France)62(7)97-101(2016 Jun)

Role of eukaryotic translation initiation factors 3a in hypoxia-induced right ventricular remodeling of rats.
Li WQ, Li XH, Wu YH, Du J, Wang AP, Li D, Li YJ
Life sciences14461-8(2016 Jan)

eIF3 targets cell-proliferation messenger RNAs for translational activation or repression.
Lee AS, Kranzusch PJ, Cate JH
Nature522(7554)111-4(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-497 could suppress TGFbeta1-induced pulmonary fibroblast proliferation and EIF3A, Collagen I and alpha-SMA protein levels.
Title: The Role of miR-497/EIF3A Axis in TGF?1-Induced Epithelial-Mesenchymal Transition and Extracellular Matrix in Rat Alveolar Epithelial Cells and Pulmonary Fibroblasts.

silencing eIF3a might alleviate TGF-beta1-induced cardiac fibrogenesis in cardiac fibroblasts by inhibiting Smad3 activation.
Title: Knockdown of eIF3a ameliorates cardiac fibrosis by inhibiting the TGF-?1/Smad3 signaling pathway.

The eIF3a plays a crucial role in hypoxia-induced right ventricular remodeling by regulating TGF-beta1-induced proliferation and differentiation of cardiac fibroblasts
Title: Role of eukaryotic translation initiation factors 3a in hypoxia-induced right ventricular remodeling of rats.

The following Eif3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09958	NM_001047087.2 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 3, subunit A (Eif3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$916.30 $1309.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif3a ( NM_001047087.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001040552.2 Rattus norvegicus eukaryotic translation initiation factor 3, subunit A (Eif3a), mRNA.

Price & Availability↑

CloneID	ORa09958
Clone ID Related Accession (Same CDS sequence)	NM_001047087.2
Accession Version	NM_001047087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic translation initiation factor 3 subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB698626.1 and AB259154.1. On Oct 15, 2009 this sequence version replaced NM_001047087.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ221906.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGCCT ATTTCCAGAG GCCGGAAAAT GCCCTCAAGC GCGCCAACGA ATTTCTTGAG 
GTTGGCAAAA AGCAGCCTGC CCTGGATGTT CTTTATGATG TTATGAAAAG TAAGAAACAC 
AGAACATGGC AGAAGATACA CGAGCCAATT ATGCTGAAAT ACTTGGAACT ATGTGTGGAT 
CTTCGTAAGA GCCACTTGGC TAAGGAAGGA TTATATCAAT ATAAGAACAT TTGTCAGCAG 
GTAAACATTA AATCTTTAGA AGATGTTGTT AGGGCATATT TGAAATTAGC AGAGGAAAAA 
ACAGAAGCTG CTAAAGAAGA GTCCCAACAA ATGGTGTTAG ACATAGAAGA TCTGGATAAT 
ATTCAGACTC CTGAGAGTGT TCTCTTAAGT GCAGTAAGTG GGGAAGACAC TCAGGATCGT 
ACTGACAGAT TGCTACTGAC TCCCTGGGTC AAGTTCCTGT GGGAATCATA CAGGCAGTGT 
TTGGACCTTC TTCGAAACAA TTCTAGAGTA GAGCGTCTTT ACCATGATAT CGCCCAACAA 
GCTTTCAAAT TCTGCCTCCA GTATACTCGC AAGGCTGAGT TCCGCAAGCT GTGTGACAAC 
TTGCGAATGC ACTTATCCCA GATTCAGCGC CACCATAACC AAAGTACAGC AATCAATCTT 
AATAATCCAG AGAGCCAGTC CATGCATTTG GAAACCAGAC TTGTTCAGCT GGACAGTGCT 
ATCAGCATGG AATTGTGGCA GGAAGCCTTC AAAGCTGTGG AAGATATTCA TGGACTATTT 
TCCTTGTCTA AAAAACCACC TAAGCCTCAG TTGATGGCAA ATTACTATAA CAAAGTTTCA 
ACGGTGTTTT GGAAATCTGG AAATGCTTTG TTTCATGCAT CTACACTTCA TCGTCTTTAT 
CATCTGTCTA GAGAAATGAG AAAGAATCTT ACACAAGATG AGATGCAAAG AATGTCTACT 
AGAGTCCTTT TGGCGACTCT TTCCATTCCT ATTACTCCTG AGCGAACTGA TATTGCACGA 
CTTTTGGATA TGGATGGTAT TATAGTTGAG AAGCAGCGGC GCCTTGCCAC ATTGCTAGGT 
CTTCAAGCAC CACCCACACG AATTGGACTT ATTAATGATA TGGTCAGGTT TAATGTGCTA 
CAATATGTTG TCCCAGAAGT GAAAGACCTT TACAACTGGT TGGAGGTGGA ATTCAACCCA 
CTAAAACTCT GTGAGAGAGT TACAAAGGTA TTAAATTGGG TTAGGGAACA ACCTGAAAAA 
GAACCAGAAT TGCAACAATA TGTGCCACAA CTACAGAACA ATACCATACT CCGTCTTCTG 
CAACAGGTGG CACAGATTTA TCAGAGCATT GAGTTTTCTC GTTTGACCTC TCTGGTTCCT 
TTTGTTGATG CTTTCCAACT GGAACGGGCC ATAGTAGATG CAGCTAGGCA CTGTGACCTG 
CAGGTTCGTA TTGACCATAC TTCTCGGACT CTGAGTTTTG GATCAGACTT AAATTATGCA 
ACTCGAGAAG ATGCCCCAAT TGGTCCTCAT CTGCAGAGCA TGCCTTCAGA ACAAATAAGA 
AATCAACTGA CAGCCATGTC CTCGGTACTT GCAAAAGCGC TTGAAGTCAT CAAGCCTGCT 
CATATTCTGC AAGAGAAAGA AGAACAGCAT CAGTTGGCAG TTACTGCGTA TATTAAAAAT 
TCACGGAAAG AGCATCAGAG GATCCTGGCT CGGAGACAGA CAATTGAGGA GAGAAAAGAG 
CGTCTTGAGA GTCTGAATAT TCAACGTGAG AAGGAAGAAC TAGAGCAGAG GGAAGCTGAA 
CTCCAGAAAG TACGGAAGGC TGAAGAAGAA AGGCTGCGTC AAGAGGCAAA GGAGCGAGAG 
AAGGAACGGA TCCTTCAAGA ACATGAACAA ATCAAGAAGA AAACTGTTCG GGAGCGGTTA 
GAACAGATCA AGAAGACAGA GCTGGGTGCC AAAGCATTTA AAGATATTGA CATTGAAGAC 
CTTGAAGAAT TGGATCCAGA TTTTATTATG GCCAAACAAG TTGAACAACT AGAAAAAGAA 
AAGAAGGAAC TTCAGGAACG CCTTAAGAAT CAAGAAAAGA AGATTGACTA TTTTGAGAGA 
GCTAAGCGTT TGGAAGAAAT TCCTTTAATA AAGAGTGCTT ACGAGGAACA AAGGGTTAAA 
GACATGGACC TGTGGGAACA ACAAGAAGAA GAAAGAATTA CTACAATGCA GCTAGAACGA 
GAAAAAGCTC TGGAACATAA GAATCGGATG TCACGAATGT TGGAAGACAG AGATCTATTT 
GTGATGCGGC TCAAAGCTGC CCGGCAGTCT GTCTATGAGG AGAAACTGAA ACAGTTTGAA 
GAGCGTTTAG CAGAAGAAAG GCATAATCGC CTGGAAGAAC GGAAGAGGCA ACGGAAAGAA 
GAACGCAAAA TAACTTACTA CAGAGAAAAG GAAGAGGAAG AGCAGAGAAG GGCAGAGGAG 
CAGATGCTCA AAGAGCGAGA AGAAAGAGAA CGAGCTGAGA GAGCAAAACG TGAGGAAGAA 
CTTAGAGAAT ATCAGGAGCG AGTCAAGAAA CTGGAAGAAG TAGAAAGGAA AAAGCGGCAA 
AGAGAGCTGG AAATTGAAGA AAGGGAAAGA CGCAGAGAGG AAGAAAGGAG ACTTGGTGAG 
GATCCACTTT CTAGGAAGGA CTCTCGTTGG GGAGATAGAG ATTCAGAAGG CACCTGGAGA 
AAAGGACCAG AAGCTGACTC TGAGTGGAGA AGAGGCCCAC CAGAAAAGGA GTGGAGACGA 
GAAACTCGGG ATGATGAGAG GCCTCACAGG AGAGATGAGG ATCGGCTGAG GCGCTTGGGG 
GGGGACGATG AAGAGAGAGA GTCGTCTCTT AGACCAGATG ATGATCGCAT CCCCAGGCGT 
GGCCTGGATG ATGACAGAGG GCCTAGACGT GGTCCCGATG AAGATAGATT TTCTCGTAGA 
GGAACAGATG ATGACCGTCC TTCCTGGCGT AATGCAGATG ATGACCGGCC TCCCAGGCGA 
ATTGGTGATG ATGACAGGGG AAGCTGGCGT CACACAGATG ATGACAGACC ACCCAGACGG 
GGACTGGATG ATGACAGACC ACCCAGACGG GGACTGGATG ATGAGAGAGG AAGCTGGCGC 
ACAGCAGAAG AGGACAGAGG ACCTAGACGT GGGATGGATG ATGACCGGGG GCCAAGACGA 
GGAGGTGCTG ATGATGAGCG GTCATCCTGG CGGAATGCTG ATGACGATAG GGGTCCCAGG 
AGGGGGATGG ATGACGATAG GGGTCCCAGG CGAGGGTTGG ATGATGATCG AGGACCTTGG 
AGGAATGCTG CTGAAGATAG GATTTCCAGG AGAGGTGCAG ATGATGACCG GGGGCCTTGG 
AGAAATATGG ATGACGATCG GGTTCCTAGA CGGGGTGATG ATGCAAGACC TGGTCCCTGG 
AGACCATTTG TCAAACCAGG TGGATGGAGA GAGAAAGAAA AGGCTAGAGA AGAGAGTTGG 
GGTCCACCTC GAGAATCAAG ACCACCAGAA GAACGTGAAT GGGATAGAGA CAAAGAGAAG 
GACAGAGATA ATCAAGATCG AGAGGAGAAC GACAAGGACC TTGAACGTGA TAGGGACAGA 
GAGAGAGATG GGGACCGGGA GGATCGTTTC AGAAGACCCA GGGATGAAGG TGGCTGGAGA 
AGAGGACCAG CAGAAGAATC TTCAAGCTGG AGAGATTCAA GTCGCCGTGA TGATAGGGAC 
AGGGATGACC GACGGCGGGA CAGAGATGAT CGTCGTGATC TGAGAGACCT GAGAGACAGA 
AGGGATTTAA GAGATGATAG AGATCGGAGA GGCCCTCCCC TTAGATCAGA GCGAGAAGAA 
GCAAGCTCCT GGAGACGCAC TGATGACAGG AAAGATGACC GGACTGAAGA AAGGGATCCA 
CCTCGTCGTG TTCCTCCCCC AACTCTTTCA AGAGAGAGAG AAAGAGAGAG AGATCGAGAA 
GGTGAGAAAG AGAAAGCATC TTGGAGAGCT GAGAAAGATA GGGAGTCCCT TCGTCGTACT 
AAGAATGAAA CTGATGAAGA TGGATGGACC ACAGTACGAC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001040552.2
CDS	185..4249
Translation	MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKLAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVAQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYIKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRVKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHNRLEERKRQRKEERKITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGEDPLSRKDSRWGDRDSEGTWRKGPEADSEWRRGPPEKEWRRETRDDERPHRRDEDRLRRLGGDDEERESSLRPDDDRIPRRGLDDDRGPRRGPDEDRFSRRGTDDDRPSWRNADDDRPPRRIGDDDRGSWRHTDDDRPPRRGLDDDRPPRRGLDDERGSWRTAEEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGMDDDRGPRRGLDDDRGPWRNAAEDRISRRGADDDRGPWRNMDDDRVPRRGDDARPGPWRPFVKPGGWREKEKAREESWGPPRESRPPEEREWDRDKEKDRDNQDREENDKDLERDRDRERDGDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRDRDDRRDLRDLRDRRDLRDDRDRRGPPLRSEREEASSWRRTDDRKDDRTEERDPPRRVPPPTLSRERERERDREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR

Target ORF information:

RefSeq Version	NM_001047087.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 3, subunit A (Eif3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047087.2

ORF Insert Sequence:

ATGCCGGCCT ATTTCCAGAG GCCGGAAAAT GCCCTCAAGC GCGCCAACGA ATTTCTTGAG 
GTTGGCAAAA AGCAGCCTGC CCTGGATGTT CTTTATGATG TTATGAAAAG TAAGAAACAC 
AGAACATGGC AGAAGATACA CGAGCCAATT ATGCTGAAAT ACTTGGAACT ATGTGTGGAT 
CTTCGTAAGA GCCACTTGGC TAAGGAAGGA TTATATCAAT ATAAGAACAT TTGTCAGCAG 
GTAAACATTA AATCTTTAGA AGATGTTGTT AGGGCATATT TGAAATTAGC AGAGGAAAAA 
ACAGAAGCTG CTAAAGAAGA GTCCCAACAA ATGGTGTTAG ACATAGAAGA TCTGGATAAT 
ATTCAGACTC CTGAGAGTGT TCTCTTAAGT GCAGTAAGTG GGGAAGACAC TCAGGATCGT 
ACTGACAGAT TGCTACTGAC TCCCTGGGTC AAGTTCCTGT GGGAATCATA CAGGCAGTGT 
TTGGACCTTC TTCGAAACAA TTCTAGAGTA GAGCGTCTTT ACCATGATAT CGCCCAACAA 
GCTTTCAAAT TCTGCCTCCA GTATACTCGC AAGGCTGAGT TCCGCAAGCT GTGTGACAAC 
TTGCGAATGC ACTTATCCCA GATTCAGCGC CACCATAACC AAAGTACAGC AATCAATCTT 
AATAATCCAG AGAGCCAGTC CATGCATTTG GAAACCAGAC TTGTTCAGCT GGACAGTGCT 
ATCAGCATGG AATTGTGGCA GGAAGCCTTC AAAGCTGTGG AAGATATTCA TGGACTATTT 
TCCTTGTCTA AAAAACCACC TAAGCCTCAG TTGATGGCAA ATTACTATAA CAAAGTTTCA 
ACGGTGTTTT GGAAATCTGG AAATGCTTTG TTTCATGCAT CTACACTTCA TCGTCTTTAT 
CATCTGTCTA GAGAAATGAG AAAGAATCTT ACACAAGATG AGATGCAAAG AATGTCTACT 
AGAGTCCTTT TGGCGACTCT TTCCATTCCT ATTACTCCTG AGCGAACTGA TATTGCACGA 
CTTTTGGATA TGGATGGTAT TATAGTTGAG AAGCAGCGGC GCCTTGCCAC ATTGCTAGGT 
CTTCAAGCAC CACCCACACG AATTGGACTT ATTAATGATA TGGTCAGGTT TAATGTGCTA 
CAATATGTTG TCCCAGAAGT GAAAGACCTT TACAACTGGT TGGAGGTGGA ATTCAACCCA 
CTAAAACTCT GTGAGAGAGT TACAAAGGTA TTAAATTGGG TTAGGGAACA ACCTGAAAAA 
GAACCAGAAT TGCAACAATA TGTGCCACAA CTACAGAACA ATACCATACT CCGTCTTCTG 
CAACAGGTGG CACAGATTTA TCAGAGCATT GAGTTTTCTC GTTTGACCTC TCTGGTTCCT 
TTTGTTGATG CTTTCCAACT GGAACGGGCC ATAGTAGATG CAGCTAGGCA CTGTGACCTG 
CAGGTTCGTA TTGACCATAC TTCTCGGACT CTGAGTTTTG GATCAGACTT AAATTATGCA 
ACTCGAGAAG ATGCCCCAAT TGGTCCTCAT CTGCAGAGCA TGCCTTCAGA ACAAATAAGA 
AATCAACTGA CAGCCATGTC CTCGGTACTT GCAAAAGCGC TTGAAGTCAT CAAGCCTGCT 
CATATTCTGC AAGAGAAAGA AGAACAGCAT CAGTTGGCAG TTACTGCGTA TATTAAAAAT 
TCACGGAAAG AGCATCAGAG GATCCTGGCT CGGAGACAGA CAATTGAGGA GAGAAAAGAG 
CGTCTTGAGA GTCTGAATAT TCAACGTGAG AAGGAAGAAC TAGAGCAGAG GGAAGCTGAA 
CTCCAGAAAG TACGGAAGGC TGAAGAAGAA AGGCTGCGTC AAGAGGCAAA GGAGCGAGAG 
AAGGAACGGA TCCTTCAAGA ACATGAACAA ATCAAGAAGA AAACTGTTCG GGAGCGGTTA 
GAACAGATCA AGAAGACAGA GCTGGGTGCC AAAGCATTTA AAGATATTGA CATTGAAGAC 
CTTGAAGAAT TGGATCCAGA TTTTATTATG GCCAAACAAG TTGAACAACT AGAAAAAGAA 
AAGAAGGAAC TTCAGGAACG CCTTAAGAAT CAAGAAAAGA AGATTGACTA TTTTGAGAGA 
GCTAAGCGTT TGGAAGAAAT TCCTTTAATA AAGAGTGCTT ACGAGGAACA AAGGGTTAAA 
GACATGGACC TGTGGGAACA ACAAGAAGAA GAAAGAATTA CTACAATGCA GCTAGAACGA 
GAAAAAGCTC TGGAACATAA GAATCGGATG TCACGAATGT TGGAAGACAG AGATCTATTT 
GTGATGCGGC TCAAAGCTGC CCGGCAGTCT GTCTATGAGG AGAAACTGAA ACAGTTTGAA 
GAGCGTTTAG CAGAAGAAAG GCATAATCGC CTGGAAGAAC GGAAGAGGCA ACGGAAAGAA 
GAACGCAAAA TAACTTACTA CAGAGAAAAG GAAGAGGAAG AGCAGAGAAG GGCAGAGGAG 
CAGATGCTCA AAGAGCGAGA AGAAAGAGAA CGAGCTGAGA GAGCAAAACG TGAGGAAGAA 
CTTAGAGAAT ATCAGGAGCG AGTCAAGAAA CTGGAAGAAG TAGAAAGGAA AAAGCGGCAA 
AGAGAGCTGG AAATTGAAGA AAGGGAAAGA CGCAGAGAGG AAGAAAGGAG ACTTGGTGAG 
GATCCACTTT CTAGGAAGGA CTCTCGTTGG GGAGATAGAG ATTCAGAAGG CACCTGGAGA 
AAAGGACCAG AAGCTGACTC TGAGTGGAGA AGAGGCCCAC CAGAAAAGGA GTGGAGACGA 
GAAACTCGGG ATGATGAGAG GCCTCACAGG AGAGATGAGG ATCGGCTGAG GCGCTTGGGG 
GGGGACGATG AAGAGAGAGA GTCGTCTCTT AGACCAGATG ATGATCGCAT CCCCAGGCGT 
GGCCTGGATG ATGACAGAGG GCCTAGACGT GGTCCCGATG AAGATAGATT TTCTCGTAGA 
GGAACAGATG ATGACCGTCC TTCCTGGCGT AATGCAGATG ATGACCGGCC TCCCAGGCGA 
ATTGGTGATG ATGACAGGGG AAGCTGGCGT CACACAGATG ATGACAGACC ACCCAGACGG 
GGACTGGATG ATGACAGACC ACCCAGACGG GGACTGGATG ATGAGAGAGG AAGCTGGCGC 
ACAGCAGAAG AGGACAGAGG ACCTAGACGT GGGATGGATG ATGACCGGGG GCCAAGACGA 
GGAGGTGCTG ATGATGAGCG GTCATCCTGG CGGAATGCTG ATGACGATAG GGGTCCCAGG 
AGGGGGATGG ATGACGATAG GGGTCCCAGG CGAGGGTTGG ATGATGATCG AGGACCTTGG 
AGGAATGCTG CTGAAGATAG GATTTCCAGG AGAGGTGCAG ATGATGACCG GGGGCCTTGG 
AGAAATATGG ATGACGATCG GGTTCCTAGA CGGGGTGATG ATGCAAGACC TGGTCCCTGG 
AGACCATTTG TCAAACCAGG TGGATGGAGA GAGAAAGAAA AGGCTAGAGA AGAGAGTTGG 
GGTCCACCTC GAGAATCAAG ACCACCAGAA GAACGTGAAT GGGATAGAGA CAAAGAGAAG 
GACAGAGATA ATCAAGATCG AGAGGAGAAC GACAAGGACC TTGAACGTGA TAGGGACAGA 
GAGAGAGATG GGGACCGGGA GGATCGTTTC AGAAGACCCA GGGATGAAGG TGGCTGGAGA 
AGAGGACCAG CAGAAGAATC TTCAAGCTGG AGAGATTCAA GTCGCCGTGA TGATAGGGAC 
AGGGATGACC GACGGCGGGA CAGAGATGAT CGTCGTGATC TGAGAGACCT GAGAGACAGA 
AGGGATTTAA GAGATGATAG AGATCGGAGA GGCCCTCCCC TTAGATCAGA GCGAGAAGAA 
GCAAGCTCCT GGAGACGCAC TGATGACAGG AAAGATGACC GGACTGAAGA AAGGGATCCA 
CCTCGTCGTG TTCCTCCCCC AACTCTTTCA AGAGAGAGAG AAAGAGAGAG AGATCGAGAA 
GGTGAGAAAG AGAAAGCATC TTGGAGAGCT GAGAAAGATA GGGAGTCCCT TCGTCGTACT 
AAGAATGAAA CTGATGAAGA TGGATGGACC ACAGTACGAC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Role of miR-497/EIF3A Axis in TGF?1-Induced Epithelial-Mesenchymal Transition and Extracellular Matrix in Rat Alveolar Epithelial Cells and Pulmonary Fibroblasts.
Journal of cellular biochemistry118(10)3401-3408(2017 Oct)
Guo R,Lv Y,Ouyang Y,Liu S,Li D

eIF3d is an mRNA cap-binding protein that is required for specialized translation initiation.
Nature536(7614)96-9(2016 08)
Lee AS,Kranzusch PJ,Doudna JA,Cate JH

Knockdown of eIF3a ameliorates cardiac fibrosis by inhibiting the TGF-?1/Smad3 signaling pathway.
Cellular and molecular biology (Noisy-le-Grand, France)62(7)97-101(2016 Jun)
Li B,Chen H,Yang X,Wang Y,Qin L,Chu Y

Role of eukaryotic translation initiation factors 3a in hypoxia-induced right ventricular remodeling of rats.
Life sciences14461-8(2016 Jan)
Li WQ,Li XH,Wu YH,Du J,Wang AP,Li D,Li YJ

eIF3 targets cell-proliferation messenger RNAs for translational activation or repression.
Nature522(7554)111-4(2015 Jun)
Lee AS,Kranzusch PJ,Cate JH

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Eif3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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