Aars cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aars
Entrez Gene ID	292023
Full Name	alanyl-tRNA synthetase
General protein information	Preferred Names alanyl-tRNA synthetase Names alanine--tRNA ligase, cytoplasmic alaRS alanyl-tRNA synthetase, cytoplasmic
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255582.3	XP_006255644.1	alanine--tRNA ligase, cytoplasmic isoform X1
NM_001100517.1	NP_001093987.1	alanine--tRNA ligase, cytoplasmic
XM_006255581.2	XP_006255643.1	alanine--tRNA ligase, cytoplasmic isoform X1
XM_006255580.3	XP_006255642.1	alanine--tRNA ligase, cytoplasmic isoform X1
XM_006255583.3	XP_006255645.1	alanine--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AARS	XP_001169520.2
Caenorhabditis elegans (roundworm)	aars-2	NP_491281.1
Neurospora crassa	NCU02566	XP_965697.2
Xenopus tropicalis (tropical clawed frog)	aars	XP_002931707.1
	AARS	XP_002808351.1
Mus musculus (house mouse)	Aars	NP_666329.2
Danio rerio (zebrafish)	aars	NP_001037775.1
Oryza sativa (rice)	Os10g0182000	NP_001064254.1
Canis lupus familiaris (dog)	AARS	XP_536788.2
Gallus gallus (chicken)	AARS	NP_001005836.1
Drosophila melanogaster (fruit fly)	Aats-ala	NP_523511.2
Kluyveromyces lactis	KLLA0F02431g	XP_455190.1
Schizosaccharomyces pombe (fission yeast)	ala1	NP_593640.1
Homo sapiens (human)	AARS	NP_001596.2
Bos taurus (cattle)	AARS	NP_001094545.1
Rattus norvegicus (Norway rat)	Aars	NP_001093987.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009701	XP_318757.4
Saccharomyces cerevisiae (baker's yeast)	ALA1	NP_014980.3
Eremothecium gossypii	AGOS_ADR363C	NP_984459.1
Magnaporthe oryzae (rice blast fungus)	MGG_03607	XP_003716291.1
Arabidopsis thaliana (thale cress)	ALATS	NP_001185186.1

Gene Ontology
Bibliography

Related articles in PubMed

Deficient activity of alanyl-tRNA synthetase underlies an autosomal recessive syndrome of progressive microcephaly, hypomyelination, and epileptic encephalopathy.
Nakayama T, Wu J, Galvin-Parton P, Weiss J, Andriola MR, Hill RS, Vaughan DJ, El-Quessny M, Barry BJ, Partlow JN, Barkovich AJ, Ling J, Mochida GH
Human mutation38(10)1348-1354(2017 Oct)

Evolutionary Gain of Alanine Mischarging to Noncognate tRNAs with a G4:U69 Base Pair.
Sun L, Gomes AC, He W, Zhou H, Wang X, Pan DW, Schimmel P, Pan T, Yang XL
Journal of the American Chemical Society138(39)12948-12955(2016 Oct)

Deficiencies in tRNA synthetase editing activity cause cardioproteinopathy.
Liu Y, Satz JS, Vo MN, Nangle LA, Schimmel P, Ackerman SL
Proceedings of the National Academy of Sciences of the United States of America111(49)17570-5(2014 Dec)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Aars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00340	NM_001100517.1 Latest version!	Rattus norvegicus alanyl-tRNA synthetase (Aars), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa00340	XM_006255581.2 Latest version!	Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa00340	XM_006255582.3 Latest version!	Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa00340	XM_006255583.3 Latest version!	Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa00340	XM_006255580.3 Latest version!	Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aars ( NM_001100517.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001093987.1 Rattus norvegicus alanyl-tRNA synthetase (Aars), mRNA.

Price & Availability↑

CloneID	ORa00340
Clone ID Related Accession (Same CDS sequence)	XM_006255582.3 , NM_001100517.1 , XM_006255581.2 , XM_006255580.3 , XM_006255583.3
Accession Version	NM_001100517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	alanine--tRNA ligase, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473972.1. On or before Oct 22, 2008 this sequence version replaced XM_001077503.1, XM_214690.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093987.1
CDS	94..3000
Translation	MDSTLTAREIRERFINYFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTVDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEAKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDTDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCQTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRAKGLEATDDSPKYNYHSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIFDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLRVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGDADQKGSLVAPDRLRFDFTAKGAMSTEQIKKTEEIVNGMIEAAKPVYTLDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEVKVKAQSAPNKDVQKEIADLGEVLATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

Target ORF information:

RefSeq Version	NM_001100517.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alanyl-tRNA synthetase (Aars), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100517.1

ORF Insert Sequence:

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aars ( XM_006255582.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255644.1 Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa00340
Clone ID Related Accession (Same CDS sequence)	XM_006255582.3 , NM_001100517.1 , XM_006255581.2 , XM_006255580.3 , XM_006255583.3
Accession Version	XM_006255582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alanine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255582.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255644.1
CDS	364..3270
Translation	MDSTLTAREIRERFINYFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTVDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEAKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDTDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCQTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRAKGLEATDDSPKYNYHSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIFDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLRVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGDADQKGSLVAPDRLRFDFTAKGAMSTEQIKKTEEIVNGMIEAAKPVYTLDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEVKVKAQSAPNKDVQKEIADLGEVLATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

Target ORF information:

RefSeq Version	XM_006255582.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255582.3

ORF Insert Sequence:

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aars ( XM_006255581.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255643.1 Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa00340
Clone ID Related Accession (Same CDS sequence)	XM_006255582.3 , NM_001100517.1 , XM_006255581.2 , XM_006255580.3 , XM_006255583.3
Accession Version	XM_006255581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alanine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006255581.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255643.1
CDS	189..3095
Translation	MDSTLTAREIRERFINYFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTVDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEAKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDTDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCQTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRAKGLEATDDSPKYNYHSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIFDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLRVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGDADQKGSLVAPDRLRFDFTAKGAMSTEQIKKTEEIVNGMIEAAKPVYTLDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEVKVKAQSAPNKDVQKEIADLGEVLATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

Target ORF information:

RefSeq Version	XM_006255581.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255581.2

ORF Insert Sequence:

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aars ( XM_006255580.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255642.1 Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00340
Clone ID Related Accession (Same CDS sequence)	XM_006255582.3 , NM_001100517.1 , XM_006255581.2 , XM_006255580.3 , XM_006255583.3
Accession Version	XM_006255580.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alanine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255580.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255642.1
CDS	368..3274
Translation	MDSTLTAREIRERFINYFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTVDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEAKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDTDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCQTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRAKGLEATDDSPKYNYHSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIFDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLRVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGDADQKGSLVAPDRLRFDFTAKGAMSTEQIKKTEEIVNGMIEAAKPVYTLDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEVKVKAQSAPNKDVQKEIADLGEVLATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

Target ORF information:

RefSeq Version	XM_006255580.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255580.3

ORF Insert Sequence:

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aars ( XM_006255583.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255645.1 Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X4, mRNA.

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CloneID	ORa00340
Clone ID Related Accession (Same CDS sequence)	XM_006255582.3 , NM_001100517.1 , XM_006255581.2 , XM_006255580.3 , XM_006255583.3
Accession Version	XM_006255583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alanine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255645.1
CDS	76..2982
Translation	MDSTLTAREIRERFINYFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTVDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEAKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDTDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDCQTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRAKGLEATDDSPKYNYHSDSSGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIFDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLRVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVLGDADQKGSLVAPDRLRFDFTAKGAMSTEQIKKTEEIVNGMIEAAKPVYTLDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEVKVKAQSAPNKDVQKEIADLGEVLATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDMSAQATGKNVGCLQEALQLATSFAQLRLGDVKN

Target ORF information:

RefSeq Version	XM_006255583.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alanyl-tRNA synthetase (Aars), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255583.3

ORF Insert Sequence:

ATGGACTCCA CTCTAACAGC ACGAGAGATC CGAGAGCGGT TTATAAATTA TTTCAAACGA 
AATGAGCACA CCTATGTCCA CTCCTCCGCC ACCATCCCTC TGGACGACCC CACCTTGCTC 
TTCGCCAACG CAGGCATGAA CCAGTTCAAA CCCATCTTCC TAAACACAGT AGACCCTTCT 
CACCCCATGG CAAAGCTGAG CAGAGCTGCC AATACCCAGA AGTGCATCCG AGCCGGGGGC 
AAACACAATG ACCTGGATGA CGTGGGCAAA GATGTCTACC ACCACACCTT CTTTGAGATG 
CTGGGCTCCT GGTCTTTTGG GGACTATTTT AAGGAATTGG CATGTAAGAT GGCTCTGGAA 
CTTCTTACCC AGGAGTTTGG TATTCCAGTT GAAAGACTCT ATGTTACTTA CTTTGGTGGG 
GATGAGGCAG CCGGCCTAGA GCCAGATCTG GAGTGTAGAC AGATCTGGCA AAATTTAGGA 
CTGGATGAAG CCAAAATTCT CCCTGGCAAC ATGAAGGACA ACTTCTGGGA GATGGGTGAC 
ACGGGCCCCT GTGGTCCCTG CAGTGAGATC CACTATGACC GCATTGGTGG CAGGGACGCT 
GCACACCTTG TCAACCAGGA TGACCCCAAT GTGCTAGAGA TCTGGAATCT CGTGTTCATC 
CAATATAACA GGGAATCTGA TGGTGTTCTG AAACCTCTCC CCAAGAAAAG CATTGACACA 
GGGATGGGCC TGGAGAGATT GGTGTCTGTG CTGCAGAACA AGATGTCCAA CTATGACACT 
GACCTTTTCG TTCCTTACTT CGAAGCCATT CAGAAGGGTA CAGGCGCCCG GCCGTATACT 
GGGAAGGTTG GTGCTGAGGA CACTGATGGA ATTGACATGG CCTACAGGGT TCTGGCTGAC 
CACGCCCGGA CCATCACTGT GGCGCTGGCT GATGGCGGGC GACCTGACAA CACAGGTCGG 
GGGTATGTGC TGAGACGGAT CCTTCGCCGA GCTGTTCGCT ATTCCCACGA GAAACTGAAC 
GCCAGCAGGG GTTTCTTCGC CACATTAGTT GATGTTGTCG TTCAATCCCT GGGAGACGCC 
TTTCCTGAGC TGAAGAAGGA CCCAGATATG GTGAAGGACA TCATTAATGA AGAAGAGGTA 
CAGTTTCTCA AGACTCTCAG CAGAGGGCGG CGCATCCTGG ACCGGAAAAT TCAGAGCTTA 
GGAGACTGCC AAACCATCCC AGGTGATACC GCTTGGCTCC TCTATGATAC TTACGGATTC 
CCAGTGGATC TCACTGGATT GATTGCTGAA GAGAAGGGCC TGGTGGTAGA TATGGATGGC 
TTTGAAGAAG AGAGGAAACT GGCCCAGCTG AAGTCACAGG GCAAGGGGGC AGGGGGCGAA 
GACCTCATCA TGCTGGACAT TTATGCGATT GAAGAACTCC GGGCAAAGGG TTTGGAGGCC 
ACAGATGATT CTCCAAAGTA TAACTACCAT TCAGACTCCA GTGGTAGCTA TGTGTTTGAA 
TGTACAGTGG CTACCGTGCT TGCTCTGCGT AGGGAAAAGA TGTTTGTGGA CGAGGTGGTC 
ACTGGCCAGG AGTGCGGAGT GGTGCTGGAC AAGACCTGTT TCTATGCCGA GCAAGGAGGG 
CAGATCTTCG ATGAAGGCTA CTTGGTGAAG GTCGACGACA GCAGTGAGGA TAAAACTGAG 
TTTACAGTGA AGAATGCTCA GGTCCGAGGC GGGTATGTGC TGCACATAGG AACAATATAT 
GGTAACCTGA GAGTGGGGGA TCAAGTCCGG CTGTTCATTG ACGAGCCCCG ACGAAGACCT 
GTCATGAGCA ACCATACAGC TACACACATC CTGAACTTCG CCTTGCGCTC GGTGCTTGGG 
GATGCAGACC AGAAAGGTTC ACTGGTTGCT CCTGACCGCC TTCGGTTTGA CTTCACTGCC 
AAGGGAGCCA TGTCCACGGA GCAAATCAAG AAGACTGAGG AGATTGTCAA TGGCATGATT 
GAGGCAGCTA AGCCTGTCTA CACCCTGGAT TGTCCCCTGG CTGCAGCAAA AGCCATCCAG 
GGCCTTCGAG CTGTATTTGA TGAGACCTAT CCTGACCCTG TGCGAGTTGT CTCCATTGGA 
GTCCCAGTGT CAGAACTGCT AGATGACCCC TCCGGGCCTG CCGGCTCCCT TACTTCTGTT 
GAGTTTTGTG GAGGAACACA TCTACGGAAT TCTAGCCATG CAGGAGCTTT CGTGATAGTG 
ACTGAAGAAG CCATTGCCAA GGGTATCCGC AGGATTGTTG CTGTCACAGG TGCTGAAGCC 
CAGAAGGCCC TCAGGAAGTC AGAAACCTTG AAGAAATCTC TCTCGGCCAT GGAGGTCAAA 
GTGAAGGCCC AGTCTGCACC CAACAAGGAT GTGCAGAAGG AAATTGCTGA CCTTGGTGAG 
GTCCTGGCCA CTGCAGTCAT TCCCCAGTGG CAGAAAGATG AACAACGGGA GACTTTGAAA 
TCCCTGAAGA AAGTCATGGA TGACCTAGAC CGGGCCAGCA AAGCCGATGT CCAGAAGCGA 
GTGTTAGAGA AGACTAAGCA GCTGATTGAC AGCAACCCCA ACCAGCCTCT GGTCATCCTG 
GAGATGGAGA GCGGTGCATC GGCCAAGGCC CTGAATGAAG CTTTGAAGCT TTTCAAGACA 
CATTCTCCTC AGACATCTGC AATGCTCTTC ACAGTAGACA ATGAGGCTGG CAAGATCACA 
TGCTTGTGTC AGGTTCCTCA GAATGCAGCC AACCGGGGGC TAAAAGCCAG CGAGTGGGTC 
CAGCAGGTTT CAGGCCTGAT GGATGGTAAA GGTGGTGGGA AGGACATGTC TGCCCAGGCC 
ACAGGCAAGA ACGTGGGCTG CCTTCAGGAA GCACTGCAGC TGGCTACTTC CTTTGCCCAG 
CTCCGTCTCG GAGATGTAAA GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficient activity of alanyl-tRNA synthetase underlies an autosomal recessive syndrome of progressive microcephaly, hypomyelination, and epileptic encephalopathy.
Human mutation38(10)1348-1354(2017 Oct)
Nakayama T,Wu J,Galvin-Parton P,Weiss J,Andriola MR,Hill RS,Vaughan DJ,El-Quessny M,Barry BJ,Partlow JN,Barkovich AJ,Ling J,Mochida GH

Evolutionary Gain of Alanine Mischarging to Noncognate tRNAs with a G4:U69 Base Pair.
Journal of the American Chemical Society138(39)12948-12955(2016 Oct)
Sun L,Gomes AC,He W,Zhou H,Wang X,Pan DW,Schimmel P,Pan T,Yang XL

Deficiencies in tRNA synthetase editing activity cause cardioproteinopathy.
Proceedings of the National Academy of Sciences of the United States of America111(49)17570-5(2014 Dec)
Liu Y,Satz JS,Vo MN,Nangle LA,Schimmel P,Ackerman SL

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

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Aars cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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