gRNAs
The following Cnot1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cnot1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa03643 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001134840.1 | |
| Accession Version | NM_001134840.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-31 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | CCR4-NOT transcription complex subunit 1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07042803.1. On Sep 9, 2008 this sequence version replaced XM_001060702.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001128312.1 |
| CDS | 307..7437 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001134840.1 |
1 | ATGAATCTTG ACTCGCTCTC GCTGGCCTTG TCTCAAATCA GCTACCTGGT GGACAATTTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. |
Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression. |
Structural basis for the recruitment of the human CCR4-NOT deadenylase complex by tristetraprolin. |
RNA decay machines: deadenylation by the Ccr4-not and Pan2-Pan3 complexes. |
The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets. |
