Ska1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ska1
Entrez Gene ID	291441
Full Name	spindle and kinetochore associated complex subunit 1
Synonyms	RGD1310784
General protein information	Preferred Names spindle and kinetochore associated complex subunit 1 Names spindle and kinetochore-associated protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772108.1	XP_008770330.1	spindle and kinetochore-associated protein 1 isoform X2
NM_001106134.1	NP_001099604.1	spindle and kinetochore-associated protein 1
XM_008772106.1	XP_008770328.1	spindle and kinetochore-associated protein 1 isoform X1
XM_008772107.1	XP_008770329.1	spindle and kinetochore-associated protein 1 isoform X1
XM_006254920.3	XP_006254982.1	spindle and kinetochore-associated protein 1 isoform X4
XM_006254918.3	XP_006254980.1	spindle and kinetochore-associated protein 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SKA1	XP_512132.2
	SKA1	XP_001093574.1
Mus musculus (house mouse)	Ska1	NP_079857.3
Rattus norvegicus (Norway rat)	Ska1	NP_001099604.1
Xenopus tropicalis (tropical clawed frog)	ska1	NP_001119997.1
Homo sapiens (human)	SKA1	NP_001034624.1
Canis lupus familiaris (dog)	SKA1	XP_548812.1
Bos taurus (cattle)	SKA1	NP_001068795.2
Danio rerio (zebrafish)	ska1	NP_001002592.1

Gene Ontology
Bibliography

Related articles in PubMed

The human kinetochore Ska1 complex facilitates microtubule depolymerization-coupled motility.
Welburn JP, Grishchuk EL, Backer CB, Wilson-Kubalek EM, Yates JR 3rd, Cheeseman IM
Developmental cell16(3)374-85(2009 Mar)

Whole genome functional analysis identifies novel components required for mitotic spindle integrity in human cells.
Rines DR, Gomez-Ferreria MA, Zhou Y, DeJesus P, Grob S, Batalov S, Labow M, Huesken D, Mickanin C, Hall J, Reinhardt M, Natt F, Lange J, Sharp DJ, Chanda SK, Caldwell JS
Genome biology9(2)R44(2008)

Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2.
Hanisch A, Sillj? HH, Nigg EA
The EMBO journal25(23)5504-15(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ska1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ska1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00243	NM_001106134.1 Latest version!	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa00243	XM_006254920.3 Latest version!	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa38499	XM_006254918.3 Latest version!	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa44951	XM_008772107.1 Latest version!	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa44951	XM_008772106.1 Latest version!	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa44952	XM_008772108.1 Latest version!	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ska1 ( NM_001106134.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099604.1 Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), mRNA.

Price & Availability↑

CloneID	ORa00243
Clone ID Related Accession (Same CDS sequence)	NM_001106134.1 , XM_006254920.3
Accession Version	NM_001106134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Rattus norvegicus(Norway rat)
Product	spindle and kinetochore-associated protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474095.2. On or before Oct 4, 2007 this sequence version replaced XM_001053750.1, XM_214527.3. ##Evidence-Data-START## Transcript exon combination :: BC158659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689604 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCGA CTCATAAGCC AGATCTGGAT CCTAAAGAGT CAGTTAAAGC TGAGGAGCCT 
GTACTGCCAA AGAAGCCTCC CAAAGAGCAG AGAGTTATTA AGGAAATGCA CTTTATAACC 
ACGGATGAGT TCAGTGGCGT ACCTGCGTAC ATGAAATCCC GTTTAACTTA TTGTCAAATT 
AATGATGTTA TCAAGGAAAT TAACAAGGCA GTAGTTAGTA AATATAAGAT TATACATCAA 
CCAAAAGCAT CTATGAGCTC TGTGAAGAGA AATCTCTACC AAAGATTCAT TAATGAAGAA 
ACGAAGGACA CGAAAGGTCG TCATTTCATC GTGGAAGCTG ACATAAAGGA GTTCACGACT 
TTGAAAGTTG ACAAGAAGTT TCATGTGATC ATGAACATAC TGCGCCATTG CCAGAGACTG 
TCGGAGGTCC GAGGCGGAGG ACTTACCCGA TATGTCATAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099604.1
CDS	196..960
Translation	MASDLEQLCSYVNEKIENIKKILSLRKLGQDPTLKTTLSKIGDEIITVNELLNQFELEIQYQEQTNSSLKELCKSLEEEFKDVEHLKEHIPSHLPQVTVTQSSTHKPDLDPKESVKAEEPVLPKKPPKEQRVIKEMHFITTDEFSGVPAYMKSRLTYCQINDVIKEINKAVVSKYKIIHQPKASMSSVKRNLYQRFINEETKDTKGRHFIVEADIKEFTTLKVDKKFHVIMNILRHCQRLSEVRGGGLTRYVIT

Target ORF information:

RefSeq Version	NM_001106134.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106134.1

ORF Insert Sequence:

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCGA CTCATAAGCC AGATCTGGAT CCTAAAGAGT CAGTTAAAGC TGAGGAGCCT 
GTACTGCCAA AGAAGCCTCC CAAAGAGCAG AGAGTTATTA AGGAAATGCA CTTTATAACC 
ACGGATGAGT TCAGTGGCGT ACCTGCGTAC ATGAAATCCC GTTTAACTTA TTGTCAAATT 
AATGATGTTA TCAAGGAAAT TAACAAGGCA GTAGTTAGTA AATATAAGAT TATACATCAA 
CCAAAAGCAT CTATGAGCTC TGTGAAGAGA AATCTCTACC AAAGATTCAT TAATGAAGAA 
ACGAAGGACA CGAAAGGTCG TCATTTCATC GTGGAAGCTG ACATAAAGGA GTTCACGACT 
TTGAAAGTTG ACAAGAAGTT TCATGTGATC ATGAACATAC TGCGCCATTG CCAGAGACTG 
TCGGAGGTCC GAGGCGGAGG ACTTACCCGA TATGTCATAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ska1 ( XM_006254920.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254982.1 Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa00243
Clone ID Related Accession (Same CDS sequence)	NM_001106134.1 , XM_006254920.3
Accession Version	XM_006254920.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spindle and kinetochore-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254920.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCGA CTCATAAGCC AGATCTGGAT CCTAAAGAGT CAGTTAAAGC TGAGGAGCCT 
GTACTGCCAA AGAAGCCTCC CAAAGAGCAG AGAGTTATTA AGGAAATGCA CTTTATAACC 
ACGGATGAGT TCAGTGGCGT ACCTGCGTAC ATGAAATCCC GTTTAACTTA TTGTCAAATT 
AATGATGTTA TCAAGGAAAT TAACAAGGCA GTAGTTAGTA AATATAAGAT TATACATCAA 
CCAAAAGCAT CTATGAGCTC TGTGAAGAGA AATCTCTACC AAAGATTCAT TAATGAAGAA 
ACGAAGGACA CGAAAGGTCG TCATTTCATC GTGGAAGCTG ACATAAAGGA GTTCACGACT 
TTGAAAGTTG ACAAGAAGTT TCATGTGATC ATGAACATAC TGCGCCATTG CCAGAGACTG 
TCGGAGGTCC GAGGCGGAGG ACTTACCCGA TATGTCATAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254982.1
CDS	203..967
Translation	MASDLEQLCSYVNEKIENIKKILSLRKLGQDPTLKTTLSKIGDEIITVNELLNQFELEIQYQEQTNSSLKELCKSLEEEFKDVEHLKEHIPSHLPQVTVTQSSTHKPDLDPKESVKAEEPVLPKKPPKEQRVIKEMHFITTDEFSGVPAYMKSRLTYCQINDVIKEINKAVVSKYKIIHQPKASMSSVKRNLYQRFINEETKDTKGRHFIVEADIKEFTTLKVDKKFHVIMNILRHCQRLSEVRGGGLTRYVIT

Target ORF information:

RefSeq Version	XM_006254920.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254920.3

ORF Insert Sequence:

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCGA CTCATAAGCC AGATCTGGAT CCTAAAGAGT CAGTTAAAGC TGAGGAGCCT 
GTACTGCCAA AGAAGCCTCC CAAAGAGCAG AGAGTTATTA AGGAAATGCA CTTTATAACC 
ACGGATGAGT TCAGTGGCGT ACCTGCGTAC ATGAAATCCC GTTTAACTTA TTGTCAAATT 
AATGATGTTA TCAAGGAAAT TAACAAGGCA GTAGTTAGTA AATATAAGAT TATACATCAA 
CCAAAAGCAT CTATGAGCTC TGTGAAGAGA AATCTCTACC AAAGATTCAT TAATGAAGAA 
ACGAAGGACA CGAAAGGTCG TCATTTCATC GTGGAAGCTG ACATAAAGGA GTTCACGACT 
TTGAAAGTTG ACAAGAAGTT TCATGTGATC ATGAACATAC TGCGCCATTG CCAGAGACTG 
TCGGAGGTCC GAGGCGGAGG ACTTACCCGA TATGTCATAA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ska1 ( XM_006254918.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254980.1 Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa38499
Clone ID Related Accession (Same CDS sequence)	XM_006254918.3
Accession Version	XM_006254918.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spindle and kinetochore-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254918.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCGA CTCATAAGCC AGATCTGGAT CCTAAAGAGT CAGTTAAAGC TGAGGAGCCT 
GTACTGCCAA AGAAGCCTCC CAAAGAGCAG AGAGTTATTA AGGAAATGCA CTTTATAACC 
ACGGATGAGT TCAGTGGCGT ACCTGCGTAC ATGAAATCCC GTTTAACTTA TTGTCAAATT 
AATGATGTTA TCAAGGAAAT TAACAAGGCA GTAGTTAGTA AATATAAGAT TATACATCAA 
CCAAAAGCAT CTATGAGCTC TGTGAAGAGA AATCTCTACC AAAGATTCAT TAATGAAGAA 
ACGAAGGACA CGAAAGGTAA AGCGATGGCC TCTGAGGGCG TCAGCTGTTG GAGAATAGCA 
GAGAAATGCA GCTGTTCTAG AGACCGCTGC CCCAGCAGTT ACAACTGTGC CACACATCAC 
CACGCTCCCG GTAGCGTGCC AGGCTTTAGT CCTCGCCACA CCCCTGTCCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254980.1
CDS	210..983
Translation	MASDLEQLCSYVNEKIENIKKILSLRKLGQDPTLKTTLSKIGDEIITVNELLNQFELEIQYQEQTNSSLKELCKSLEEEFKDVEHLKEHIPSHLPQVTVTQSSTHKPDLDPKESVKAEEPVLPKKPPKEQRVIKEMHFITTDEFSGVPAYMKSRLTYCQINDVIKEINKAVVSKYKIIHQPKASMSSVKRNLYQRFINEETKDTKGKAMASEGVSCWRIAEKCSCSRDRCPSSYNCATHHHAPGSVPGFSPRHTPVP

Target ORF information:

RefSeq Version	XM_006254918.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254918.3

ORF Insert Sequence:

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCGA CTCATAAGCC AGATCTGGAT CCTAAAGAGT CAGTTAAAGC TGAGGAGCCT 
GTACTGCCAA AGAAGCCTCC CAAAGAGCAG AGAGTTATTA AGGAAATGCA CTTTATAACC 
ACGGATGAGT TCAGTGGCGT ACCTGCGTAC ATGAAATCCC GTTTAACTTA TTGTCAAATT 
AATGATGTTA TCAAGGAAAT TAACAAGGCA GTAGTTAGTA AATATAAGAT TATACATCAA 
CCAAAAGCAT CTATGAGCTC TGTGAAGAGA AATCTCTACC AAAGATTCAT TAATGAAGAA 
ACGAAGGACA CGAAAGGTAA AGCGATGGCC TCTGAGGGCG TCAGCTGTTG GAGAATAGCA 
GAGAAATGCA GCTGTTCTAG AGACCGCTGC CCCAGCAGTT ACAACTGTGC CACACATCAC 
CACGCTCCCG GTAGCGTGCC AGGCTTTAGT CCTCGCCACA CCCCTGTCCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ska1 ( XM_008772106.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770328.1 Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44951
Clone ID Related Accession (Same CDS sequence)	XM_008772106.1 , XM_008772107.1
Accession Version	XM_008772106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spindle and kinetochore-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCTT TAAAAAGCAT GTTCAGTCTG CTGCTTAATT CTGACACTTG GCTGTCTGCA 
CGTCCATCTC TGTTCTTTAG GACTCATAAG CCAGATCTGG ATCCTAAAGA GTCAGTTAAA 
GCTGAGGAGC CTGTACTGCC AAAGAAGCCT CCCAAAGAGC AGAGAGTTAT TAAGGAAATG 
CACTTTATAA CCACGGATGA GTTCAGTGGC GTACCTGCGT ACATGAAATC CCGTTTAACT 
TATTGTCAAA TTAATGATGT TATCAAGGAA ATTAACAAGG CAGTAGTTAG TAAATATAAG 
ATTATACATC AACCAAAAGC ATCTATGAGC TCTGTGAAGA GAAATCTCTA CCAAAGATTC 
ATTAATGAAG AAACGAAGGA CACGAAAGGT AAAGCGATGG CCTCTGAGGG CGTCAGCTGT 
TGGAGAATAG CAGAGAAATG CAGCTGTTCT AGAGACCGCT GCCCCAGCAG TTACAACTGT 
GCCACACATC ACCACGCTCC CGGTAGCGTG CCAGGCTTTA GTCCTCGCCA CACCCCTGTC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770328.1
CDS	212..1057
Translation	MASDLEQLCSYVNEKIENIKKILSLRKLGQDPTLKTTLSKIGDEIITVNELLNQFELEIQYQEQTNSSLKELCKSLEEEFKDVEHLKEHIPSHLPQVTVTQSSLKSMFSLLLNSDTWLSARPSLFFRTHKPDLDPKESVKAEEPVLPKKPPKEQRVIKEMHFITTDEFSGVPAYMKSRLTYCQINDVIKEINKAVVSKYKIIHQPKASMSSVKRNLYQRFINEETKDTKGKAMASEGVSCWRIAEKCSCSRDRCPSSYNCATHHHAPGSVPGFSPRHTPVP

Target ORF information:

RefSeq Version	XM_008772106.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772106.1

ORF Insert Sequence:

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCTT TAAAAAGCAT GTTCAGTCTG CTGCTTAATT CTGACACTTG GCTGTCTGCA 
CGTCCATCTC TGTTCTTTAG GACTCATAAG CCAGATCTGG ATCCTAAAGA GTCAGTTAAA 
GCTGAGGAGC CTGTACTGCC AAAGAAGCCT CCCAAAGAGC AGAGAGTTAT TAAGGAAATG 
CACTTTATAA CCACGGATGA GTTCAGTGGC GTACCTGCGT ACATGAAATC CCGTTTAACT 
TATTGTCAAA TTAATGATGT TATCAAGGAA ATTAACAAGG CAGTAGTTAG TAAATATAAG 
ATTATACATC AACCAAAAGC ATCTATGAGC TCTGTGAAGA GAAATCTCTA CCAAAGATTC 
ATTAATGAAG AAACGAAGGA CACGAAAGGT AAAGCGATGG CCTCTGAGGG CGTCAGCTGT 
TGGAGAATAG CAGAGAAATG CAGCTGTTCT AGAGACCGCT GCCCCAGCAG TTACAACTGT 
GCCACACATC ACCACGCTCC CGGTAGCGTG CCAGGCTTTA GTCCTCGCCA CACCCCTGTC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ska1 ( XM_008772107.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770329.1 Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44951
Clone ID Related Accession (Same CDS sequence)	XM_008772106.1 , XM_008772107.1
Accession Version	XM_008772107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spindle and kinetochore-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCTT TAAAAAGCAT GTTCAGTCTG CTGCTTAATT CTGACACTTG GCTGTCTGCA 
CGTCCATCTC TGTTCTTTAG GACTCATAAG CCAGATCTGG ATCCTAAAGA GTCAGTTAAA 
GCTGAGGAGC CTGTACTGCC AAAGAAGCCT CCCAAAGAGC AGAGAGTTAT TAAGGAAATG 
CACTTTATAA CCACGGATGA GTTCAGTGGC GTACCTGCGT ACATGAAATC CCGTTTAACT 
TATTGTCAAA TTAATGATGT TATCAAGGAA ATTAACAAGG CAGTAGTTAG TAAATATAAG 
ATTATACATC AACCAAAAGC ATCTATGAGC TCTGTGAAGA GAAATCTCTA CCAAAGATTC 
ATTAATGAAG AAACGAAGGA CACGAAAGGT AAAGCGATGG CCTCTGAGGG CGTCAGCTGT 
TGGAGAATAG CAGAGAAATG CAGCTGTTCT AGAGACCGCT GCCCCAGCAG TTACAACTGT 
GCCACACATC ACCACGCTCC CGGTAGCGTG CCAGGCTTTA GTCCTCGCCA CACCCCTGTC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770329.1
CDS	208..1053
Translation	MASDLEQLCSYVNEKIENIKKILSLRKLGQDPTLKTTLSKIGDEIITVNELLNQFELEIQYQEQTNSSLKELCKSLEEEFKDVEHLKEHIPSHLPQVTVTQSSLKSMFSLLLNSDTWLSARPSLFFRTHKPDLDPKESVKAEEPVLPKKPPKEQRVIKEMHFITTDEFSGVPAYMKSRLTYCQINDVIKEINKAVVSKYKIIHQPKASMSSVKRNLYQRFINEETKDTKGKAMASEGVSCWRIAEKCSCSRDRCPSSYNCATHHHAPGSVPGFSPRHTPVP

Target ORF information:

RefSeq Version	XM_008772107.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772107.1

ORF Insert Sequence:

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCTT TAAAAAGCAT GTTCAGTCTG CTGCTTAATT CTGACACTTG GCTGTCTGCA 
CGTCCATCTC TGTTCTTTAG GACTCATAAG CCAGATCTGG ATCCTAAAGA GTCAGTTAAA 
GCTGAGGAGC CTGTACTGCC AAAGAAGCCT CCCAAAGAGC AGAGAGTTAT TAAGGAAATG 
CACTTTATAA CCACGGATGA GTTCAGTGGC GTACCTGCGT ACATGAAATC CCGTTTAACT 
TATTGTCAAA TTAATGATGT TATCAAGGAA ATTAACAAGG CAGTAGTTAG TAAATATAAG 
ATTATACATC AACCAAAAGC ATCTATGAGC TCTGTGAAGA GAAATCTCTA CCAAAGATTC 
ATTAATGAAG AAACGAAGGA CACGAAAGGT AAAGCGATGG CCTCTGAGGG CGTCAGCTGT 
TGGAGAATAG CAGAGAAATG CAGCTGTTCT AGAGACCGCT GCCCCAGCAG TTACAACTGT 
GCCACACATC ACCACGCTCC CGGTAGCGTG CCAGGCTTTA GTCCTCGCCA CACCCCTGTC 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ska1 ( XM_008772108.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770330.1 Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44952
Clone ID Related Accession (Same CDS sequence)	XM_008772108.1
Accession Version	XM_008772108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spindle and kinetochore-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCTT TAAAAAGCAT GTTCAGTCTG CTGCTTAATT CTGACACTTG GCTGTCTGCA 
CGTCCATCTC TGTTCTTTAG GACTCATAAG CCAGATCTGG ATCCTAAAGA GTCAGTTAAA 
GCTGAGGAGC CTGTACTGCC AAAGAAGCCT CCCAAAGAGC AGAGAGTTAT TAAGGAAATG 
CACTTTATAA CCACGGATGA GTTCAGTGGC GTACCTGCGT ACATGAAATC CCGTTTAACT 
TATTGTCAAA TTAATGATGT TATCAAGGAA ATTAACAAGG CAGTAGTTAG TAAATATAAG 
ATTATACATC AACCAAAAGC ATCTATGAGC TCTGTGAAGA GAAATCTCTA CCAAAGATTC 
ATTAATGAAG AAACGAAGGA CACGAAAGGT CGTCATTTCA TCGTGGAAGC TGACATAAAG 
GAGTTCACGA CTTTGAAAGT TGACAAGAAG TTTCATGTGA TCATGAACAT ACTGCGCCAT 
TGCCAGAGAC TGTCGGAGGT CCGAGGCGGA GGACTTACCC GATATGTCAT AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770330.1
CDS	210..1046
Translation	MASDLEQLCSYVNEKIENIKKILSLRKLGQDPTLKTTLSKIGDEIITVNELLNQFELEIQYQEQTNSSLKELCKSLEEEFKDVEHLKEHIPSHLPQVTVTQSSLKSMFSLLLNSDTWLSARPSLFFRTHKPDLDPKESVKAEEPVLPKKPPKEQRVIKEMHFITTDEFSGVPAYMKSRLTYCQINDVIKEINKAVVSKYKIIHQPKASMSSVKRNLYQRFINEETKDTKGRHFIVEADIKEFTTLKVDKKFHVIMNILRHCQRLSEVRGGGLTRYVIT

Target ORF information:

RefSeq Version	XM_008772108.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772108.1

ORF Insert Sequence:

ATGGCCTCAG ATCTAGAACA GCTATGCTCT TATGTTAATG AGAAGATTGA GAATATTAAG 
AAAATTCTGT CGTTAAGAAA ACTTGGCCAA GACCCTACCT TGAAGACCAC CTTGAGTAAA 
ATAGGAGATG AGATCATTAC GGTCAATGAG CTTCTAAATC AATTTGAATT GGAAATTCAG 
TATCAAGAAC AAACTAACAG TTCACTCAAG GAGCTCTGCA AATCTCTTGA AGAAGAGTTT 
AAAGATGTGG AACATCTCAA AGAACACATT CCTTCCCATT TGCCTCAAGT GACAGTGACC 
CAGAGCTCTT TAAAAAGCAT GTTCAGTCTG CTGCTTAATT CTGACACTTG GCTGTCTGCA 
CGTCCATCTC TGTTCTTTAG GACTCATAAG CCAGATCTGG ATCCTAAAGA GTCAGTTAAA 
GCTGAGGAGC CTGTACTGCC AAAGAAGCCT CCCAAAGAGC AGAGAGTTAT TAAGGAAATG 
CACTTTATAA CCACGGATGA GTTCAGTGGC GTACCTGCGT ACATGAAATC CCGTTTAACT 
TATTGTCAAA TTAATGATGT TATCAAGGAA ATTAACAAGG CAGTAGTTAG TAAATATAAG 
ATTATACATC AACCAAAAGC ATCTATGAGC TCTGTGAAGA GAAATCTCTA CCAAAGATTC 
ATTAATGAAG AAACGAAGGA CACGAAAGGT CGTCATTTCA TCGTGGAAGC TGACATAAAG 
GAGTTCACGA CTTTGAAAGT TGACAAGAAG TTTCATGTGA TCATGAACAT ACTGCGCCAT 
TGCCAGAGAC TGTCGGAGGT CCGAGGCGGA GGACTTACCC GATATGTCAT AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The human kinetochore Ska1 complex facilitates microtubule depolymerization-coupled motility.
Developmental cell16(3)374-85(2009 Mar)
Welburn JP,Grishchuk EL,Backer CB,Wilson-Kubalek EM,Yates JR 3rd,Cheeseman IM

Whole genome functional analysis identifies novel components required for mitotic spindle integrity in human cells.
Genome biology9(2)R44(2008)
Rines DR,Gomez-Ferreria MA,Zhou Y,DeJesus P,Grob S,Batalov S,Labow M,Huesken D,Mickanin C,Hall J,Reinhardt M,Natt F,Lange J,Sharp DJ,Chanda SK,Caldwell JS

Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2.
The EMBO journal25(23)5504-15(2006 Nov)
Hanisch A,Sillj? HH,Nigg EA

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Ska1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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