Actn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actn2
Entrez Gene ID	291245
Full Name	actinin alpha 2
General protein information	Preferred Names actinin alpha 2 Names alpha-actinin-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170325.1	NP_001163796.1	alpha-actinin-2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ACTN2	XP_001158729.2
	ACTN2	XP_001097795.1
Rattus norvegicus (Norway rat)	Actn2	NP_001163796.1
Gallus gallus (chicken)	ACTN2	NP_990654.1
Danio rerio (zebrafish)	actn2	NP_001032662.1
Xenopus tropicalis (tropical clawed frog)	actn2	NP_001005053.1
Homo sapiens (human)	ACTN2	NP_001094.1
Canis lupus familiaris (dog)	ACTN2	XP_856116.1
Bos taurus (cattle)	ACTN2	NP_001029807.1
Mus musculus (house mouse)	Actn2	NP_150371.4

Gene Ontology
Bibliography

Related articles in PubMed

Z-disc protein CHAPb induces cardiomyopathy and contractile dysfunction in the postnatal heart.
van Eldik W, den Adel B, Monshouwer-Kloots J, Salvatori D, Maas S, van der Made I, Creemers EE, Frank D, Frey N, Boontje N, van der Velden J, Steendijk P, Mummery C, Passier R, Beqqali A
PloS one12(12)e0189139(2017)

Kindlin-2 interacts with ?-actinin-2 and ?1 integrin to maintain the integrity of the Z-disc in cardiac muscles.
Qi L, Yu Y, Chi X, Xu W, Lu D, Song Y, Zhang Y, Zhang H
FEBS letters589(16)2155-62(2015 Jul)

The structure and regulation of human muscle ?-actinin.
Ribeiro Ede A Jr, Pinotsis N, Ghisleni A, Salmazo A, Konarev PV, Kostan J, Sj?blom B, Schreiner C, Polyansky AA, Gkougkoulia EA, Holt MR, Aachmann FL, Zagrovi? B, Bordignon E, Pirker KF, Svergun DI, Gautel M, Djinovi?-Carugo K
Cell159(6)1447-60(2014 Dec)

A cardiomyocyte-specific Wdr1 knockout demonstrates essential functional roles for actin disassembly during myocardial growth and maintenance in mice.
Yuan B, Wan P, Chu D, Nie J, Cao Y, Luo W, Lu S, Chen J, Yang Z
The American journal of pathology184(7)1967-80(2014 Jul)

?-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.
Hodges JL, Vilchez SM, Asmussen H, Whitmore LA, Horwitz AR
PloS one9(7)e101770(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that kindlin-2 (Kind2/Fermt2) interacts with actin alpha 2 (Actn2) and integrin beta 1 (Itgb1) and co-localizes to cardiac sarcomere at Z-disc; knockdown of Kind2 leads to dissociation of Actn2 and Itgb1.
Title: Kindlin-2 interacts with ?-actinin-2 and ?1 integrin to maintain the integrity of the Z-disc in cardiac muscles.

ALpha-actinin-2, partially through its calcium insensitive EF-hand motif, nucleates post-synaptic density formation via F-actin organization and modulates spine maturation to mediate synaptogenesis.
Title: ?-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.

Results support alpha-actinin adaptation at the isoform level and show that alpha-actinin-2 expression is associated with improvement of muscular aerobic capacity.
Title: Effects of ageing and endurance exercise training on alpha-actinin isoforms in rat plantaris muscle.

In contrast to alpha-actinin-3, exercise training increased alpha-actinin-2 expression levels in skeletal muscles, apparently related to the change to muscle with higher aerobic capacity.
Title: Different adaptations of alpha-actinin isoforms to exercise training in rat skeletal muscles.

CAMKII isoforms interact with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2
Title: Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B, densin-180, and alpha-actinin-2.

The following Actn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06135	NM_001170325.1 Latest version!	Rattus norvegicus actinin alpha 2 (Actn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actn2 ( NM_001170325.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001163796.1 Rattus norvegicus actinin alpha 2 (Actn2), mRNA.

Price & Availability↑

CloneID	ORa06135
Clone ID Related Accession (Same CDS sequence)	NM_001170325.1
Accession Version	NM_001170325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	alpha-actinin-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK355337.1, DY315226.1, CK358853.1, CK600096.1, FM118105.1, FM123616.1 and CR461659.1. On or before Dec 18, 2009 this sequence version replaced XM_214499.4, XM_001061664.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAATCAGA TAGAGCCCGG CGTGCAGTAC AACTATGTCT ACGACGAAGA TGAGTACATG 
ATCCAGGAGG AGGAGTGGGA CCGCGACCTG CTCCTCGACC CGGCCTGGGA GAAGCAGCAG 
AGGAAGACTT TCACTGCCTG GTGCAACTCG CACCTGCGCA AAGCCGGCAC CCAGATCGAG 
AACATAGAGG AGGATTTCAG GAATGGCCTC AAGCTCATGC TGCTTCTGGA AGTCATCTCA 
GGGGAAAGGC TTCCCAAACC TGACCGGGGC AAAATGCGGT TCCACAAGAT TGCGAATGTC 
AACAAGGCTT TGGATTACAT AGCCAGCAAG GGAGTGAAAT TGGTATCTAT TGGGGCTGAA 
GAAATCGTCG ATGGCAATGT GAAGATGACC CTGGGTATGA TCTGGACCAT CATCCTCCGC 
TTCGCCATTC AGGACATCTC AGTGGAAGAA ACATCTGCCA AAGAGGGCCT GCTGCTATGG 
TGTCAGAGGA AGACAGCACC ATACCGAAAT GTGAACATCC AGAACTTCCA CACCAGCTGG 
AAAGATGGCC TTGGACTCTG TGCCCTCATC CACAGACACC GGCCTGACCT CATCGACTAC 
TCAAAGCTTA ACAAGGATGA CCCCATAGGA AACATTAACC TGGCCATGGA AATAGCAGAG 
AAGCACTTGG ATATCCCTAA AATGTTGGAT GCTGAAGACA TTGTCAACAC TCCCAAACCC 
GATGAAAGAG CCATCATGAC TTACGTTTCC TGCTTCTACC ATGCTTTCGC GGGAGCGGAG 
CAGGCCGAGA CGGCAGCTAA CAGGATATGT AAGGTTCTTG CTGTGAATCA AGAGAATGAG 
AGGCTGATGG AAGAATATGA GAGGCTAGCG AGTGAGCTCT TGGAATGGAT TCGACGCACA 
ATCCCCTGGC TGGAGAACAG GACACCGGAG AAGACCATGC AAGCCATGCA GAAGAAGCTG 
GAGGACTTCC GGGACTATCG TCGTAAGCAC AAGCCCCCAA AGGTGCAGGA GAAGTGCCAG 
CTGGAGATCA ACTTCAACAC CCTGCAGACC AAGCTTCGGA TCAGCAACCG GCCTGCCTTC 
ATGCCCTCCG AGGGCAAGAT GGTGTCGGAC ATTGCTGGTG CCTGGCAGAG GTTGGAGCAG 
GCCGAGAAGG GCTATGAGGA ATGGCTATTG AATGAGATTC GAAGACTGGA GCGCTTGGAG 
CACTTGGCTG AGAAGTTCAG GCAGAAGGCC TCCACCCATG AGACATGGGC TTACGGCAAA 
GAACAGATCT TGCTGCAGAA GGACTATGAG TCTGCGTCCC TGACGGAAGT TCGAGCCCTA 
CTTCGGAAGC ACGAGGCCTT TGAGAGTGAC CTGGCGGCAC ACCAGGATCG TGTGGAACAG 
ATTGCAGCCA TTGCTCAGGA GCTCAATGAA CTGGACTACC ACGATGCTGT GAACGTCAAT 
GATCGTTGCC AGAAAATTTG CGACCAGTGG GATAGATTGG GGACGCTCAC CCAGAAGAGG 
AGGGAGGCCT TGGAGAGAAC CGAGAAGTTG CTAGAAACAA TTGACCAGCT GCACCTGGAG 
TTTGCCAAGA GAGCTGCGCC CTTCAACAAC TGGATGGAGG GCGCCATGGA GGACCTGCAG 
GACATGTTCA TCGTCCACAG CATTGAGGAG ATCCAGAGTC TGATCACCGC ACATGAGCAG 
TTCAAGGCCA CGCTGCCCGA GGCCGATGGG GAGCGGCAGT CCATCCTAGC CATCCAGAAT 
GAGGTGGAGA AGGTGATCCA GAGCTACAGT ATCCGGATCA GCTCCAGCAA CCCCTACAGC 
ACTGTCACCA TGGACGAGCT GCGCAACAAG TGGGACAAGG TGAAGCAGCT GGTGCCAGTC 
CGGGATCAGT CCCTGCAGGA GGAGCTGGCA CGCCAACATG CTAATGAGCG CCTACGGCGC 
CAGTTTGCTG CCCAGGCCAA TGCCATCGGC CCCTGGATCC AGAACAAGAT GGAGGAGATC 
GCTCGGAGCT CCATCCAGAT CACAGGGGCC CTGGAGGACC AGATGAACCA GCTGAAGCAG 
TACGAGCACA ACATCATCAA CTACAAGAAC AACATTGACA AGCTGGAGGG CGACCACCAG 
CTCATCCAGG AAGCCCTGGT CTTCGACAAC AAGCACACCA ACTACACCAT GGAGCATATC 
CGTGTCGGAT GGGAGTTGCT GCTCACAACT ATCGCCAGAA CCATCAATGA GGTGGAGACC 
CAGATCCTGA CAAGAGATGC CAAGGGCATC ACCCAGGAGC AGATGAATGA GTTCAGAGCC 
TCCTTCAACC ACTTTGACAG GAGGAAGAAT GGCCTGATGG ATCATGAGGA TTTCAGAGCC 
TGCCTCATTT CCATGGGCTA TGACTTGGGT GAAGCTGAGT TTGCCCGAAT TATGACTCTG 
GTTGACCCCA ACGGACAAGG CACGGTCACC TTCCAGTCCT TCATTGACTT CATGACTAGA 
GAGACCGCAG ACACGGACAC TGCAGAACAG GTCATCGCCT CCTTCCGGAT TCTGGCTTCT 
GATAAGCCTT ACATCTTGGC AGAGGAACTT CGTCGAGAGC TGCCTCCGGA TCAGGCCCAG 
TACTGCATCA AGAGAATGCC CCCATACTCA GGCCCAGGCA GTGTCCCCGG GGCCCTGGAC 
TACACTGCCT TCTCCTCTGC CCTCTACGGG GAGAGCGACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163796.1
CDS	190..2874
Translation	MNQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEVISGERLPKPDRGKMRFHKIANVNKALDYIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNIQNFHTSWKDGLGLCALIHRHRPDLIDYSKLNKDDPIGNINLAMEIAEKHLDIPKMLDAEDIVNTPKPDERAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENERLMEEYERLASELLEWIRRTIPWLENRTPEKTMQAMQKKLEDFRDYRRKHKPPKVQEKCQLEINFNTLQTKLRISNRPAFMPSEGKMVSDIAGAWQRLEQAEKGYEEWLLNEIRRLERLEHLAEKFRQKASTHETWAYGKEQILLQKDYESASLTEVRALLRKHEAFESDLAAHQDRVEQIAAIAQELNELDYHDAVNVNDRCQKICDQWDRLGTLTQKRREALERTEKLLETIDQLHLEFAKRAAPFNNWMEGAMEDLQDMFIVHSIEEIQSLITAHEQFKATLPEADGERQSILAIQNEVEKVIQSYSIRISSSNPYSTVTMDELRNKWDKVKQLVPVRDQSLQEELARQHANERLRRQFAAQANAIGPWIQNKMEEIARSSIQITGALEDQMNQLKQYEHNIINYKNNIDKLEGDHQLIQEALVFDNKHTNYTMEHIRVGWELLLTTIARTINEVETQILTRDAKGITQEQMNEFRASFNHFDRRKNGLMDHEDFRACLISMGYDLGEAEFARIMTLVDPNGQGTVTFQSFIDFMTRETADTDTAEQVIASFRILASDKPYILAEELRRELPPDQAQYCIKRMPPYSGPGSVPGALDYTAFSSALYGESDL

Target ORF information:

RefSeq Version	NM_001170325.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actinin alpha 2 (Actn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170325.1

ORF Insert Sequence:

ATGAATCAGA TAGAGCCCGG CGTGCAGTAC AACTATGTCT ACGACGAAGA TGAGTACATG 
ATCCAGGAGG AGGAGTGGGA CCGCGACCTG CTCCTCGACC CGGCCTGGGA GAAGCAGCAG 
AGGAAGACTT TCACTGCCTG GTGCAACTCG CACCTGCGCA AAGCCGGCAC CCAGATCGAG 
AACATAGAGG AGGATTTCAG GAATGGCCTC AAGCTCATGC TGCTTCTGGA AGTCATCTCA 
GGGGAAAGGC TTCCCAAACC TGACCGGGGC AAAATGCGGT TCCACAAGAT TGCGAATGTC 
AACAAGGCTT TGGATTACAT AGCCAGCAAG GGAGTGAAAT TGGTATCTAT TGGGGCTGAA 
GAAATCGTCG ATGGCAATGT GAAGATGACC CTGGGTATGA TCTGGACCAT CATCCTCCGC 
TTCGCCATTC AGGACATCTC AGTGGAAGAA ACATCTGCCA AAGAGGGCCT GCTGCTATGG 
TGTCAGAGGA AGACAGCACC ATACCGAAAT GTGAACATCC AGAACTTCCA CACCAGCTGG 
AAAGATGGCC TTGGACTCTG TGCCCTCATC CACAGACACC GGCCTGACCT CATCGACTAC 
TCAAAGCTTA ACAAGGATGA CCCCATAGGA AACATTAACC TGGCCATGGA AATAGCAGAG 
AAGCACTTGG ATATCCCTAA AATGTTGGAT GCTGAAGACA TTGTCAACAC TCCCAAACCC 
GATGAAAGAG CCATCATGAC TTACGTTTCC TGCTTCTACC ATGCTTTCGC GGGAGCGGAG 
CAGGCCGAGA CGGCAGCTAA CAGGATATGT AAGGTTCTTG CTGTGAATCA AGAGAATGAG 
AGGCTGATGG AAGAATATGA GAGGCTAGCG AGTGAGCTCT TGGAATGGAT TCGACGCACA 
ATCCCCTGGC TGGAGAACAG GACACCGGAG AAGACCATGC AAGCCATGCA GAAGAAGCTG 
GAGGACTTCC GGGACTATCG TCGTAAGCAC AAGCCCCCAA AGGTGCAGGA GAAGTGCCAG 
CTGGAGATCA ACTTCAACAC CCTGCAGACC AAGCTTCGGA TCAGCAACCG GCCTGCCTTC 
ATGCCCTCCG AGGGCAAGAT GGTGTCGGAC ATTGCTGGTG CCTGGCAGAG GTTGGAGCAG 
GCCGAGAAGG GCTATGAGGA ATGGCTATTG AATGAGATTC GAAGACTGGA GCGCTTGGAG 
CACTTGGCTG AGAAGTTCAG GCAGAAGGCC TCCACCCATG AGACATGGGC TTACGGCAAA 
GAACAGATCT TGCTGCAGAA GGACTATGAG TCTGCGTCCC TGACGGAAGT TCGAGCCCTA 
CTTCGGAAGC ACGAGGCCTT TGAGAGTGAC CTGGCGGCAC ACCAGGATCG TGTGGAACAG 
ATTGCAGCCA TTGCTCAGGA GCTCAATGAA CTGGACTACC ACGATGCTGT GAACGTCAAT 
GATCGTTGCC AGAAAATTTG CGACCAGTGG GATAGATTGG GGACGCTCAC CCAGAAGAGG 
AGGGAGGCCT TGGAGAGAAC CGAGAAGTTG CTAGAAACAA TTGACCAGCT GCACCTGGAG 
TTTGCCAAGA GAGCTGCGCC CTTCAACAAC TGGATGGAGG GCGCCATGGA GGACCTGCAG 
GACATGTTCA TCGTCCACAG CATTGAGGAG ATCCAGAGTC TGATCACCGC ACATGAGCAG 
TTCAAGGCCA CGCTGCCCGA GGCCGATGGG GAGCGGCAGT CCATCCTAGC CATCCAGAAT 
GAGGTGGAGA AGGTGATCCA GAGCTACAGT ATCCGGATCA GCTCCAGCAA CCCCTACAGC 
ACTGTCACCA TGGACGAGCT GCGCAACAAG TGGGACAAGG TGAAGCAGCT GGTGCCAGTC 
CGGGATCAGT CCCTGCAGGA GGAGCTGGCA CGCCAACATG CTAATGAGCG CCTACGGCGC 
CAGTTTGCTG CCCAGGCCAA TGCCATCGGC CCCTGGATCC AGAACAAGAT GGAGGAGATC 
GCTCGGAGCT CCATCCAGAT CACAGGGGCC CTGGAGGACC AGATGAACCA GCTGAAGCAG 
TACGAGCACA ACATCATCAA CTACAAGAAC AACATTGACA AGCTGGAGGG CGACCACCAG 
CTCATCCAGG AAGCCCTGGT CTTCGACAAC AAGCACACCA ACTACACCAT GGAGCATATC 
CGTGTCGGAT GGGAGTTGCT GCTCACAACT ATCGCCAGAA CCATCAATGA GGTGGAGACC 
CAGATCCTGA CAAGAGATGC CAAGGGCATC ACCCAGGAGC AGATGAATGA GTTCAGAGCC 
TCCTTCAACC ACTTTGACAG GAGGAAGAAT GGCCTGATGG ATCATGAGGA TTTCAGAGCC 
TGCCTCATTT CCATGGGCTA TGACTTGGGT GAAGCTGAGT TTGCCCGAAT TATGACTCTG 
GTTGACCCCA ACGGACAAGG CACGGTCACC TTCCAGTCCT TCATTGACTT CATGACTAGA 
GAGACCGCAG ACACGGACAC TGCAGAACAG GTCATCGCCT CCTTCCGGAT TCTGGCTTCT 
GATAAGCCTT ACATCTTGGC AGAGGAACTT CGTCGAGAGC TGCCTCCGGA TCAGGCCCAG 
TACTGCATCA AGAGAATGCC CCCATACTCA GGCCCAGGCA GTGTCCCCGG GGCCCTGGAC 
TACACTGCCT TCTCCTCTGC CCTCTACGGG GAGAGCGACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Z-disc protein CHAPb induces cardiomyopathy and contractile dysfunction in the postnatal heart.
PloS one12(12)e0189139(2017)
van Eldik W,den Adel B,Monshouwer-Kloots J,Salvatori D,Maas S,van der Made I,Creemers EE,Frank D,Frey N,Boontje N,van der Velden J,Steendijk P,Mummery C,Passier R,Beqqali A

Kindlin-2 interacts with ?-actinin-2 and ?1 integrin to maintain the integrity of the Z-disc in cardiac muscles.
FEBS letters589(16)2155-62(2015 Jul)
Qi L,Yu Y,Chi X,Xu W,Lu D,Song Y,Zhang Y,Zhang H

The structure and regulation of human muscle ?-actinin.
Cell159(6)1447-60(2014 Dec)
Ribeiro Ede A Jr,Pinotsis N,Ghisleni A,Salmazo A,Konarev PV,Kostan J,Sj?blom B,Schreiner C,Polyansky AA,Gkougkoulia EA,Holt MR,Aachmann FL,Zagrovi? B,Bordignon E,Pirker KF,Svergun DI,Gautel M,Djinovi?-Carugo K

A cardiomyocyte-specific Wdr1 knockout demonstrates essential functional roles for actin disassembly during myocardial growth and maintenance in mice.
The American journal of pathology184(7)1967-80(2014 Jul)
Yuan B,Wan P,Chu D,Nie J,Cao Y,Luo W,Lu S,Chen J,Yang Z

?-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons.
PloS one9(7)e101770(2014)
Hodges JL,Vilchez SM,Asmussen H,Whitmore LA,Horwitz AR

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Actn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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