Col4a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col4a1
Entrez Gene ID	290905
Full Name	collagen type IV alpha 1 chain
General protein information	Preferred Names collagen type IV alpha 1 chain Names collagen alpha-1(IV) chain alpha 1 type IV collagen collagen, type IV, alpha 1 procollagen, type IV, alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16q12.5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135009.1	NP_001128481.1	collagen alpha-1(IV) chain precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A1	XP_003314261.2
Mus musculus (house mouse)	Col4a1	NP_034061.2
Rattus norvegicus (Norway rat)	Col4a1	NP_001128481.1
Xenopus tropicalis (tropical clawed frog)	col4a1	XP_002933064.2
	COL4A1	XP_001088660.2
Canis lupus familiaris (dog)	COL4A1	XP_005634201.1
Danio rerio (zebrafish)	col4a1	XP_694040.5
Gallus gallus (chicken)	COL4A1	NP_001155871.1
Homo sapiens (human)	COL4A1	NP_001836.2
Bos taurus (cattle)	COL4A1	NP_001159983.1

Gene Ontology
Bibliography

Related articles in PubMed

Parathyroid hormone inhibits TGF-?/Smad signaling and extracellular matrix proteins upregulation in rat mesangial cells.
Peng FF, Xiao ZL, Chen HM, Chen Y, Zhou J, Yu H, Zhang BF
Biochemical and biophysical research communications478(3)1093-8(2016 09)

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Effects of angiotensin II intervention on MMP-2, MMP-9, TIMP-1, and collagen expression in rats with pulmonary hypertension.
Wang XM, Shi K, Li JJ, Chen TT, Guo YH, Liu YL, Yang YF, Yang S
Genetics and molecular research : GMR14(1)1707-17(2015 Mar)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PTH pretreatment prevented TGF-beta1 and high glucose-induced Smad2/3 phosphorylation and consequent upregulation of fibronectin and type IV collagen within 4 h.
Title: Parathyroid hormone inhibits TGF-?/Smad signaling and extracellular matrix proteins upregulation in rat mesangial cells.

The metabolism of individual alpha(IV) chains of Glomerular Basement Membrane is differentially regulated in diabetic conditions.
Title: Regulation of type IV collagen alpha chains of glomerular epithelial cells in diabetic conditions.

The following Col4a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03852	NM_001135009.1 Latest version!	Rattus norvegicus collagen type IV alpha 1 chain (Col4a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col4a1 ( NM_001135009.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001128481.1 Rattus norvegicus collagen type IV alpha 1 chain (Col4a1), mRNA.

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CloneID	ORa03852
Clone ID Related Accession (Same CDS sequence)	NM_001135009.1
Accession Version	NM_001135009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(IV) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07026563.1. On Sep 16, 2008 this sequence version replaced XM_214400.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCCCC GGCTCAGCGT CTGGCTTCTG CTGCCGCTCG CCGCCCTTCT GCTCCACGAG 
GAGCGCAGCC GGGCCGCTGC GAAGGGTGAT TGTGGTGGCT CTGGCTGTGG AAAATGTGAC 
TGTCATGGTG TGAAGGGACA AAAGGGAGAG AGAGGCTTTC CAGGGTTACA AGGTGTCATT 
GGATTTCCTG GGATGCAAGG ACCTGAGGGG CCACATGGAC CACCAGGACA AAAGGGTGAT 
GCTGGAGAAC CTGGACTTCC TGGAACGAAA GGGACACGAG GACCCCCTGG AGCAGCTGGC 
TACCCTGGGA ATCCCGGACT TCCTGGTATT CCTGGCCAGG ATGGTCCTCC AGGTCCCCCA 
GGTATCCCAG GATGCAATGG TACAAAGGGA GAGAGAGGGC CACTCGGTCC TCCTGGCTTG 
CCTGGATTCA GCGGGAATCC TGGACCACCG GGGTTACCAG GAATGAAGGG AGATCCCGGT 
GAAATTCTCG GCCATGTTCC TGGGACCCTG CTGAAAGGAG AGAGAGGATT TCCTGGTATC 
CCTGGGGCGC CGGGCTCACC AGGGTTGCCA GGACTCCAGG GACCTGTCGG TCCTCCGGGA 
TTTACTGGAC CACCGGGTCC TCCAGGCCCT CCTGGACCTC CTGGAGAAAA GGGGCAGATG 
GGATCCAGCT TCCAAGGACC AAAAGGCGAC AAGGGAGAAC AAGGGGTCAG TGGTCCCCCG 
GGAGTACCTG GACAGGCACA AGTTAAGGAA AAAGGAGACT TTGCCCCAAC AGGAGAAAAG 
GGTCAGAAAG GTGAACCTGG ATTCCAGGGA ACACCAGGAT ATGGAGAGAA AGGTGAACCT 
GGCAAGCCAG GACCTCGGGG AAAACCTGGA AAAGATGGTG AAAAAGGAGA AAAGGGGAGC 
CCGGGCTTTC CTGGTGAATC CGGATACCCA GGTCTTCCAG GCCGGCAGGG CCCTCAGGGA 
GAGAAGGGTG AACCTGGACT TCCAGGCCCC CCTGGAACTG TGGTAGGCAC CAGGCCATTG 
GGAGAGAAAG GAGAGCGAGG CTACCCGGGA TCCCCAGGGT TAAGAGGAGA GCCCGGTCCT 
AAAGGTTACC CTGGATCGCC AGGCCAACCA GGCCCTCCAG GTTTCGCTGT TCCAGGCCAG 
ACTGGTGCTC CGGGCTTCCC AGGTGAAAGA GGAGAAAAAG GTGAAAGGGG ATCTCCAGGT 
GTGTCTTTAC CAGGACCAAG TGGAAGAGAT GGAGCCCCAG GGCCTCCTGG CCCTCCTGGC 
CCCCCTGGGC AACCAGGCCA CACGAACGGC ATTGTGGAGT GTCAACCTGG ACCACCTGGG 
GACCAGGGTC CTCCAGGAAT TCCAGGACAG CCAGGTTTGA CAGGCGAAGT TGGACAGAAA 
GGTCAGAAAG GAGAGAGCTG CCTTGCCTGT GACACAGAAG GACTTCGAGG CCCCCCAGGG 
CCACAGGGGC CTCCAGGAGA GATTGGTTTC CCTGGACAGC CAGGGGCTAA AGGTGACCGA 
GGCCTGCCAG GCAGAGATGG TCTTGAAGGA TTGCCGGGTC CACAAGGTTC ACCGGGGCTC 
ATAGGCCAGC CTGGAGCTAA GGGAGAGCCC GGCGAGATAT TTTTTGACAT GCGACTCAAA 
GGTGACAAAG GAGACCCAGG TTTTCCAGGA CAGCCAGGGA TGCCAGGAAG AGCAGGAACT 
CCGGGAAGAG ACGGCCACCC AGGACTCCCC GGACCCAAAG GCTCCCCGGG TTCAATAGGA 
TTGAAGGGAG AGAGAGGTCC CCCTGGAGGA GTTGGCTTTC CTGGTAGTCG TGGAGACATT 
GGCCCTCCTG GACCCCCAGG AGTCGGTCCC ATCGGTCCCA TTGGTGAAAA AGGACAAGCA 
GGCCTTCCTG GGGGCCCCGG GTCCCCAGGT CTTCCAGGTC CAAAGGGTGA AGCAGGAAAG 
GTTGTCCCAC TCCCCGGCCC CCCTGGAGCT GCAGGACTTC CAGGATCCCC TGGGTTCCCA 
GGGCCACAAG GTGACCGAGG CTTCCCAGGA ACCCCAGGAC GGCCAGGCAA CCCAGGAGAG 
AAGGGTGCTG TGGGCCAACC AGGAATTGGA TTTCCCGGGC TTCCTGGCCC CAAAGGTGTT 
GATGGCTTGC CTGGAGAGAT AGGACGACCG GGGAGTCCAG GTCGCCCTGG ATTTAATGGC 
TTACCTGGCA ACCCAGGACC GCAAGGCCAA AAGGGAGAAC CTGGCATTGG GCTTCCAGGA 
CTCAAAGGGC AGCCAGGTCT TCCAGGTATC CCTGGCACAC CCGGAGAGAA AGGCAGCATC 
GGGGGACCTG GCGTTCCAGG AGAACAGGGG TTGACTGGCC CTCCCGGACT CCAGGGGATC 
AGAGGTGACC CAGGACCTCC TGGGGTTCAA GGCCCTGCAG GTCCACCAGG GGTTCCAGGA 
ATAGGACCAC CCGGAGCTAT GGGCCCTCCC GGAGGGCAAG GACCACCAGG TTCATCAGGT 
CCACCTGGAG TTAAAGGAGA GAAGGGATTC CCTGGATTCC CAGGACTGGA TATGCCTGGC 
CCCAAAGGAG ATAAAGGCTC TCAAGGACTT CCTGGCCTCA CAGGACAGTC AGGGCTCCCT 
GGCCTCCCTG GACAGCAGGG GACTCCTGGA GTTCCAGGGT TCCCAGGTTC TAAAGGTGAA 
ATGGGCGTCA TGGGAACCCC GGGACAACCA GGCTCACCAG GACCAGCAGG CGCCCCAGGG 
TTACCCGGAG AGAAAGGAGA CCATGGCTTT CCGGGCTCCT CGGGACCCAG GGGCGACCCT 
GGCTTCAAAG GTGATAAAGG TGATGTCGGG CTTCCTGGCA TGCCAGGATC CATGGAGCAC 
GTGGACATGG GCAGCATGAA GGGGCAGAAA GGAGACCAGG GAGAGAAAGG ACAAATTGGA 
CCCACTGGTG ATAAAGGTTC CCGAGGAGAC CCCGGAACAC CAGGAGTGCC CGGGAAGGAT 
GGGCAGGCAG GGCACCCTGG ACAGCCAGGA CCTAAAGGTG ACCCAGGCCT TAGTGGAATA 
CCAGGATCCC CTGGACTTCC TGGACCCAAA GGATCGGTTG GAGGAATGGG CTTGCCAGGT 
TCGCCCGGAG AGAAAGGCGT TCCGGGCATC CCTGGCTCAC AGGGTGTCCC TGGCTCACCT 
GGAGAGAAAG GAGCTAAGGG AGAGAAAGGG CAGTCAGGTC TCCCTGGCAT TGGAATTCCT 
GGACGGCCTG GTGACAAGGG AGATCAGGGG CTTGCAGGTT TCCCAGGCAG CCCTGGTGAG 
AAGGGAGAGA AAGGCAGTGC CGGAACCCCA GGGATGCCAG GGTCCCCAGG CCCAAGAGGC 
TCTCCAGGGA ACATCGGCCA TCCAGGAAGC CCAGGCCTGC CTGGAGAAAA AGGAGACAAA 
GGCCTCCCAG GACTGGATGG TGTTCCCGGT GTCAAAGGAG AAGCAGGTCT TCCTGGGACT 
CCTGGCCCCA CAGGCCCAGC TGGCCAGAAG GGAGAACCTG GCAGTGATGG AATCCCGGGG 
TCGGCAGGAG AGAAGGGTGA ACAAGGTTTG CCAGGAAGAG GCTTCCCAGG CTTCCCCGGC 
ACCAAAGGAG ACAAAGGTTC CAAGGGTGAA GTGGGTTTCC CTGGCCTTGC CGGAAGTCCT 
GGGATTCCTG GAGCCAAAGG CGAGCAAGGA TTCATGGGTC CTCCCGGCCC TCAAGGACAG 
CCGGGCTTAC CTGGCACTCC TGGCCACCCT GTGGAGGGGC CCAAAGGAGA CCGAGGACCT 
CAGGGTCAAC CCGGCCTGCC AGGGCATCCG GGACCTATGG GGCCACCAGG GTTCCCTGGA 
ATCGATGGGC CAAAAGGTGA CAAGGGAAAT CCAGGTTGGC CAGGAGCTCC TGGGGCTCCA 
GGCCCTAAGG GAGACCCAGG ATTCCAAGGC ATGCCGGGCA TTGGCGGCTC TCCAGGGATC 
ACAGGTTCAA AGGGAGATAT GGGACCGCCT GGCGTTCCAG GATTCCAAGG TCAGAAAGGT 
CTTCCTGGCT TGCAGGGAGT GAAGGGAGAT CAGGGAGATC AAGGCATACC CGGCCCTAAA 
GGTCTCCAAG GTCCCCCTGG GCCCCCAGGT CCCTACGATG TCATCAAAGG AGAACCAGGG 
CTCCCAGGTC CTGAGGGTCC TCCTGGTCTC AAAGGACTTC AAGGACCACC AGGTCCAAAA 
GGACAGCAAG GTGTGACAGG CTCCGTGGGC TTGCCTGGAC CTCCAGGTGT CCCTGGGTTT 
GATGGTGCCC CTGGCCAGAA AGGAGAGACT GGACCATTTG GACCACCTGG TCCGAGAGGG 
TTTCCTGGTC CACCAGGCCC CGATGGGCTG CCAGGATCCA TGGGTCCCCC GGGTACCCCA 
TCTGTGGACC ATGGCTTCCT TGTGACCAGG CATAGTCAGA CAACAGATGA CCCGCTGTGT 
CCCCCAGGGA CCAAAATTCT TTACCATGGG TACTCTCTGC TCTATGTCCA AGGAAACGAG 
CGGGCCCATG GTCAGGACTT GGGTACGGCT GGCAGCTGTC TGCGCAAGTT CAGCACCATG 
CCCTTCCTCT TCTGCAACAT CAACAACGTC TGCAACTTCG CCTCCAGGAA CGACTACTCC 
TACTGGCTGT CCACTCCGGA GCCCATGCCC ATGTCCATGG CACCTATCTC TGGGGACAAC 
ATCCGGCCCT TCATTAGCAG GTGTGCAGTT TGCGAAGCGC CCGCCATGGT GATGGCGGTG 
CACAGTCAGA CCATCCAGAT CCCGCAGTGC CCCAACGGTT GGTCCTCACT GTGGATTGGC 
TATTCCTTTG TGATGCACAC CAGCGCTGGT GCTGAAGGTT CTGGCCAAGC CCTTGCATCT 
CCCGGGTCCT GTCTGGAAGA GTTTAGAAGT GCCCCATTCA TCGAGTGCCA TGGCAGAGGA 
ACGTGCAATT ACTACGCGAA TGCTTACAGC TTTTGGCTCG CCACCATAGA GAGAAGCGAG 
ATGTTCAAGA AGCCCACGCC GTCCACCTTG AAGGCAGGGG AGCTGCGCAC GCATGTCAGC 
CGCTGCCAAG TGTGCATGAG AAGAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128481.1
CDS	203..5212
Translation	MGPRLSVWLLLPLAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGFPGLQGVIGFPGMQGPEGPHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGLPGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGAPGSPGLPGLQGPVGPPGFTGPPGPPGPPGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFQGTPGYGEKGEPGKPGPRGKPGKDGEKGEKGSPGFPGESGYPGLPGRQGPQGEKGEPGLPGPPGTVVGTRPLGEKGERGYPGSPGLRGEPGPKGYPGSPGQPGPPGFAVPGQTGAPGFPGERGEKGERGSPGVSLPGPSGRDGAPGPPGPPGPPGQPGHTNGIVECQPGPPGDQGPPGIPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGFPGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGTPGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPIGEKGQAGLPGGPGSPGLPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGNPGEKGAVGQPGIGFPGLPGPKGVDGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQGLTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGVKGEKGFPGFPGLDMPGPKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGAPGLPGEKGDHGFPGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKDGQAGHPGQPGPKGDPGLSGIPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKGQSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDKGLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGLPGRGFPGFPGTKGDKGSKGEVGFPGLAGSPGIPGAKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPGIDGPKGDKGNPGWPGAPGAPGPKGDPGFQGMPGIGGSPGITGSKGDMGPPGVPGFQGQKGLPGLQGVKGDQGDQGIPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGFDGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHGYSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDNIRPFISRCAVCEAPAMVMAVHSQTIQIPQCPNGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT

Target ORF information:

RefSeq Version	NM_001135009.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 1 chain (Col4a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135009.1

ORF Insert Sequence:

ATGGGGCCCC GGCTCAGCGT CTGGCTTCTG CTGCCGCTCG CCGCCCTTCT GCTCCACGAG 
GAGCGCAGCC GGGCCGCTGC GAAGGGTGAT TGTGGTGGCT CTGGCTGTGG AAAATGTGAC 
TGTCATGGTG TGAAGGGACA AAAGGGAGAG AGAGGCTTTC CAGGGTTACA AGGTGTCATT 
GGATTTCCTG GGATGCAAGG ACCTGAGGGG CCACATGGAC CACCAGGACA AAAGGGTGAT 
GCTGGAGAAC CTGGACTTCC TGGAACGAAA GGGACACGAG GACCCCCTGG AGCAGCTGGC 
TACCCTGGGA ATCCCGGACT TCCTGGTATT CCTGGCCAGG ATGGTCCTCC AGGTCCCCCA 
GGTATCCCAG GATGCAATGG TACAAAGGGA GAGAGAGGGC CACTCGGTCC TCCTGGCTTG 
CCTGGATTCA GCGGGAATCC TGGACCACCG GGGTTACCAG GAATGAAGGG AGATCCCGGT 
GAAATTCTCG GCCATGTTCC TGGGACCCTG CTGAAAGGAG AGAGAGGATT TCCTGGTATC 
CCTGGGGCGC CGGGCTCACC AGGGTTGCCA GGACTCCAGG GACCTGTCGG TCCTCCGGGA 
TTTACTGGAC CACCGGGTCC TCCAGGCCCT CCTGGACCTC CTGGAGAAAA GGGGCAGATG 
GGATCCAGCT TCCAAGGACC AAAAGGCGAC AAGGGAGAAC AAGGGGTCAG TGGTCCCCCG 
GGAGTACCTG GACAGGCACA AGTTAAGGAA AAAGGAGACT TTGCCCCAAC AGGAGAAAAG 
GGTCAGAAAG GTGAACCTGG ATTCCAGGGA ACACCAGGAT ATGGAGAGAA AGGTGAACCT 
GGCAAGCCAG GACCTCGGGG AAAACCTGGA AAAGATGGTG AAAAAGGAGA AAAGGGGAGC 
CCGGGCTTTC CTGGTGAATC CGGATACCCA GGTCTTCCAG GCCGGCAGGG CCCTCAGGGA 
GAGAAGGGTG AACCTGGACT TCCAGGCCCC CCTGGAACTG TGGTAGGCAC CAGGCCATTG 
GGAGAGAAAG GAGAGCGAGG CTACCCGGGA TCCCCAGGGT TAAGAGGAGA GCCCGGTCCT 
AAAGGTTACC CTGGATCGCC AGGCCAACCA GGCCCTCCAG GTTTCGCTGT TCCAGGCCAG 
ACTGGTGCTC CGGGCTTCCC AGGTGAAAGA GGAGAAAAAG GTGAAAGGGG ATCTCCAGGT 
GTGTCTTTAC CAGGACCAAG TGGAAGAGAT GGAGCCCCAG GGCCTCCTGG CCCTCCTGGC 
CCCCCTGGGC AACCAGGCCA CACGAACGGC ATTGTGGAGT GTCAACCTGG ACCACCTGGG 
GACCAGGGTC CTCCAGGAAT TCCAGGACAG CCAGGTTTGA CAGGCGAAGT TGGACAGAAA 
GGTCAGAAAG GAGAGAGCTG CCTTGCCTGT GACACAGAAG GACTTCGAGG CCCCCCAGGG 
CCACAGGGGC CTCCAGGAGA GATTGGTTTC CCTGGACAGC CAGGGGCTAA AGGTGACCGA 
GGCCTGCCAG GCAGAGATGG TCTTGAAGGA TTGCCGGGTC CACAAGGTTC ACCGGGGCTC 
ATAGGCCAGC CTGGAGCTAA GGGAGAGCCC GGCGAGATAT TTTTTGACAT GCGACTCAAA 
GGTGACAAAG GAGACCCAGG TTTTCCAGGA CAGCCAGGGA TGCCAGGAAG AGCAGGAACT 
CCGGGAAGAG ACGGCCACCC AGGACTCCCC GGACCCAAAG GCTCCCCGGG TTCAATAGGA 
TTGAAGGGAG AGAGAGGTCC CCCTGGAGGA GTTGGCTTTC CTGGTAGTCG TGGAGACATT 
GGCCCTCCTG GACCCCCAGG AGTCGGTCCC ATCGGTCCCA TTGGTGAAAA AGGACAAGCA 
GGCCTTCCTG GGGGCCCCGG GTCCCCAGGT CTTCCAGGTC CAAAGGGTGA AGCAGGAAAG 
GTTGTCCCAC TCCCCGGCCC CCCTGGAGCT GCAGGACTTC CAGGATCCCC TGGGTTCCCA 
GGGCCACAAG GTGACCGAGG CTTCCCAGGA ACCCCAGGAC GGCCAGGCAA CCCAGGAGAG 
AAGGGTGCTG TGGGCCAACC AGGAATTGGA TTTCCCGGGC TTCCTGGCCC CAAAGGTGTT 
GATGGCTTGC CTGGAGAGAT AGGACGACCG GGGAGTCCAG GTCGCCCTGG ATTTAATGGC 
TTACCTGGCA ACCCAGGACC GCAAGGCCAA AAGGGAGAAC CTGGCATTGG GCTTCCAGGA 
CTCAAAGGGC AGCCAGGTCT TCCAGGTATC CCTGGCACAC CCGGAGAGAA AGGCAGCATC 
GGGGGACCTG GCGTTCCAGG AGAACAGGGG TTGACTGGCC CTCCCGGACT CCAGGGGATC 
AGAGGTGACC CAGGACCTCC TGGGGTTCAA GGCCCTGCAG GTCCACCAGG GGTTCCAGGA 
ATAGGACCAC CCGGAGCTAT GGGCCCTCCC GGAGGGCAAG GACCACCAGG TTCATCAGGT 
CCACCTGGAG TTAAAGGAGA GAAGGGATTC CCTGGATTCC CAGGACTGGA TATGCCTGGC 
CCCAAAGGAG ATAAAGGCTC TCAAGGACTT CCTGGCCTCA CAGGACAGTC AGGGCTCCCT 
GGCCTCCCTG GACAGCAGGG GACTCCTGGA GTTCCAGGGT TCCCAGGTTC TAAAGGTGAA 
ATGGGCGTCA TGGGAACCCC GGGACAACCA GGCTCACCAG GACCAGCAGG CGCCCCAGGG 
TTACCCGGAG AGAAAGGAGA CCATGGCTTT CCGGGCTCCT CGGGACCCAG GGGCGACCCT 
GGCTTCAAAG GTGATAAAGG TGATGTCGGG CTTCCTGGCA TGCCAGGATC CATGGAGCAC 
GTGGACATGG GCAGCATGAA GGGGCAGAAA GGAGACCAGG GAGAGAAAGG ACAAATTGGA 
CCCACTGGTG ATAAAGGTTC CCGAGGAGAC CCCGGAACAC CAGGAGTGCC CGGGAAGGAT 
GGGCAGGCAG GGCACCCTGG ACAGCCAGGA CCTAAAGGTG ACCCAGGCCT TAGTGGAATA 
CCAGGATCCC CTGGACTTCC TGGACCCAAA GGATCGGTTG GAGGAATGGG CTTGCCAGGT 
TCGCCCGGAG AGAAAGGCGT TCCGGGCATC CCTGGCTCAC AGGGTGTCCC TGGCTCACCT 
GGAGAGAAAG GAGCTAAGGG AGAGAAAGGG CAGTCAGGTC TCCCTGGCAT TGGAATTCCT 
GGACGGCCTG GTGACAAGGG AGATCAGGGG CTTGCAGGTT TCCCAGGCAG CCCTGGTGAG 
AAGGGAGAGA AAGGCAGTGC CGGAACCCCA GGGATGCCAG GGTCCCCAGG CCCAAGAGGC 
TCTCCAGGGA ACATCGGCCA TCCAGGAAGC CCAGGCCTGC CTGGAGAAAA AGGAGACAAA 
GGCCTCCCAG GACTGGATGG TGTTCCCGGT GTCAAAGGAG AAGCAGGTCT TCCTGGGACT 
CCTGGCCCCA CAGGCCCAGC TGGCCAGAAG GGAGAACCTG GCAGTGATGG AATCCCGGGG 
TCGGCAGGAG AGAAGGGTGA ACAAGGTTTG CCAGGAAGAG GCTTCCCAGG CTTCCCCGGC 
ACCAAAGGAG ACAAAGGTTC CAAGGGTGAA GTGGGTTTCC CTGGCCTTGC CGGAAGTCCT 
GGGATTCCTG GAGCCAAAGG CGAGCAAGGA TTCATGGGTC CTCCCGGCCC TCAAGGACAG 
CCGGGCTTAC CTGGCACTCC TGGCCACCCT GTGGAGGGGC CCAAAGGAGA CCGAGGACCT 
CAGGGTCAAC CCGGCCTGCC AGGGCATCCG GGACCTATGG GGCCACCAGG GTTCCCTGGA 
ATCGATGGGC CAAAAGGTGA CAAGGGAAAT CCAGGTTGGC CAGGAGCTCC TGGGGCTCCA 
GGCCCTAAGG GAGACCCAGG ATTCCAAGGC ATGCCGGGCA TTGGCGGCTC TCCAGGGATC 
ACAGGTTCAA AGGGAGATAT GGGACCGCCT GGCGTTCCAG GATTCCAAGG TCAGAAAGGT 
CTTCCTGGCT TGCAGGGAGT GAAGGGAGAT CAGGGAGATC AAGGCATACC CGGCCCTAAA 
GGTCTCCAAG GTCCCCCTGG GCCCCCAGGT CCCTACGATG TCATCAAAGG AGAACCAGGG 
CTCCCAGGTC CTGAGGGTCC TCCTGGTCTC AAAGGACTTC AAGGACCACC AGGTCCAAAA 
GGACAGCAAG GTGTGACAGG CTCCGTGGGC TTGCCTGGAC CTCCAGGTGT CCCTGGGTTT 
GATGGTGCCC CTGGCCAGAA AGGAGAGACT GGACCATTTG GACCACCTGG TCCGAGAGGG 
TTTCCTGGTC CACCAGGCCC CGATGGGCTG CCAGGATCCA TGGGTCCCCC GGGTACCCCA 
TCTGTGGACC ATGGCTTCCT TGTGACCAGG CATAGTCAGA CAACAGATGA CCCGCTGTGT 
CCCCCAGGGA CCAAAATTCT TTACCATGGG TACTCTCTGC TCTATGTCCA AGGAAACGAG 
CGGGCCCATG GTCAGGACTT GGGTACGGCT GGCAGCTGTC TGCGCAAGTT CAGCACCATG 
CCCTTCCTCT TCTGCAACAT CAACAACGTC TGCAACTTCG CCTCCAGGAA CGACTACTCC 
TACTGGCTGT CCACTCCGGA GCCCATGCCC ATGTCCATGG CACCTATCTC TGGGGACAAC 
ATCCGGCCCT TCATTAGCAG GTGTGCAGTT TGCGAAGCGC CCGCCATGGT GATGGCGGTG 
CACAGTCAGA CCATCCAGAT CCCGCAGTGC CCCAACGGTT GGTCCTCACT GTGGATTGGC 
TATTCCTTTG TGATGCACAC CAGCGCTGGT GCTGAAGGTT CTGGCCAAGC CCTTGCATCT 
CCCGGGTCCT GTCTGGAAGA GTTTAGAAGT GCCCCATTCA TCGAGTGCCA TGGCAGAGGA 
ACGTGCAATT ACTACGCGAA TGCTTACAGC TTTTGGCTCG CCACCATAGA GAGAAGCGAG 
ATGTTCAAGA AGCCCACGCC GTCCACCTTG AAGGCAGGGG AGCTGCGCAC GCATGTCAGC 
CGCTGCCAAG TGTGCATGAG AAGAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Parathyroid hormone inhibits TGF-?/Smad signaling and extracellular matrix proteins upregulation in rat mesangial cells.
Biochemical and biophysical research communications478(3)1093-8(2016 09)
Peng FF,Xiao ZL,Chen HM,Chen Y,Zhou J,Yu H,Zhang BF

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Effects of angiotensin II intervention on MMP-2, MMP-9, TIMP-1, and collagen expression in rats with pulmonary hypertension.
Genetics and molecular research : GMR14(1)1707-17(2015 Mar)
Wang XM,Shi K,Li JJ,Chen TT,Guo YH,Liu YL,Yang YF,Yang S

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

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Col4a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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