Mphosph8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mphosph8
Entrez Gene ID	290270
Full Name	M-phase phosphoprotein 8
Synonyms	Ab2-008,RGD1305133
General protein information	Preferred Names M-phase phosphoprotein 8 Names M-phase phosphoprotein 8 M-phase phosphoprotein, mpp8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017375.2	NP_001017375.2	M-phase phosphoprotein 8

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MPHOSPH8	NP_001139453.1
Mus musculus (house mouse)	Mphosph8	NP_076262.2
Xenopus tropicalis (tropical clawed frog)	mphosph8	XP_002938003.1
Pan troglodytes (chimpanzee)	MPHOSPH8	XP_001144070.1
Rattus norvegicus (Norway rat)	Mphosph8	NP_001017375.2
Homo sapiens (human)	MPHOSPH8	NP_059990.2
Bos taurus (cattle)	MPHOSPH8	XP_002691897.2
	MPHOSPH8	XP_001117375.2
Gallus gallus (chicken)	MPHOSPH8	NP_001164636.1

Gene Ontology
Bibliography

Related articles in PubMed

Selective silencing of euchromatic L1s revealed by genome-wide screens for L1 regulators.
Liu N, Lee CH, Swigut T, Grow E, Gu B, Bassik MC, Wysocka J
Nature553(7687)228-232(2018 Jan)

Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.
Tchasovnikarova IA, Timms RT, Douse CH, Roberts RC, Dougan G, Kingston RE, Modis Y, Lehner PJ
Nature genetics49(7)1035-1044(2017 Jul)

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
Tchasovnikarova IA, Timms RT, Matheson NJ, Wals K, Antrobus R, G?ttgens B, Dougan G, Dawson MA, Lehner PJ
Science (New York, N.Y.)348(6242)1481-1485(2015 Jun)

Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.
Murata K, Sato S, Haruta M, Goshima T, Chiba Y, Takahashi S, Sharif J, Koseki H, Nakanishi M, Shimada M
Biochemical and biophysical research communications458(3)470-5(2015 Mar)

Methyl-H3K9-binding protein MPP8 mediates E-cadherin gene silencing and promotes tumour cell motility and invasion.
Kokura K, Sun L, Bedford MT, Fang J
The EMBO journal29(21)3673-87(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that MPP8 could have a role for silencing genes that are associated with differentiation of the testis and the mesoderm by interacting with epigenetic repressors modules such as the PRC1 complex.
Title: Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.

The following Mphosph8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mphosph8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04061	NM_001017375.2 Latest version!	Rattus norvegicus M-phase phosphoprotein 8 (Mphosph8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mphosph8 ( NM_001017375.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001017375.2 Rattus norvegicus M-phase phosphoprotein 8 (Mphosph8), mRNA.

Price & Availability↑

CloneID	ORa04061
Clone ID Related Accession (Same CDS sequence)	NM_001017375.2
Accession Version	NM_001017375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	M-phase phosphoprotein 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB700328.1, BC099116.1 and BP474651.1. On Oct 27, 2010 this sequence version replaced NM_001017375.1. ##Evidence-Data-START## Transcript exon combination :: BC099116.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG CTACTGAGGA AAACATGAGT GTGGCCGCGC TAGTTATGTC AGTTCCGGAC 
AACATTGGCC GATCTCCCGA GGTGGAAGGA GGTGGAGCAG CGGGCGAAGA GAAGGACGCA 
GCCACCAAGG GAACGGTGGC GGTTGGGGAC AGCGAGGAGG ATGGGGAGGA CGTTTTCGAG 
GTGGAGAGGA TCCTGGACAT GAAGTGCGAG GGAGGTAAGA ATCTTTATAA GGTTCGATGG 
AAAGGATATA CGTCTGATGA TGACACTTGG GAACCTGAGG TTCATCTGGA GGACTGTAAA 
GAAGTTCTTC TTGAATTTAG GAAGAAAGTT GCTGAGAACA AAGCTAAAGC AGTAAGGAAA 
GATATTCAGA AACTATCTTT AAACAATGAC ATATTTGAGG CAGACAGTGA TATCGACCAG 
CAAGGTGACA CAAAAGAAGA TACGTCACCA AGGAAGAAAA AGAAAAAAAT AAAGTACAAA 
GAAGATAAAA GTCCAGATGA TCTGAGGAAG AAAAGGGCAA AGATGGGAAA ACTAAAAGAT 
AAGTTCAAGA CAGAGCTGGA GAGCACCTCT GAAATTTTAG GTTTTGATGT AAAGACAAAG 
AAAAGAATTT TGGAAGTCAA AGAAGAATTA AAGGACTCCA AAAAACCCAA AAAGGATGAA 
ATAAAGGAAA CCAAGAAAAC TAAAAGGGCT GACATAAGAG ATTTAAAGAT TAAAATAAGA 
GAAGATGTCA AAGACAACCG AAAAACAAAG AAAGAAAGAT ATATAGACTC TCCCCTGGAG 
TCCGAGTCAC CTAATGATTC CTTCACTCTA GAGGATGAGA GTGAAGACTT TCTTTCCGAC 
AACAAAGAGA AACAAAATGT AAGAACTGCA AAGGATAAGA CAGGACAGGA TACAGTGCAA 
GAGAGCATTT TTGAGAAACA TCTGGATGAC CTTATAAGTA TTGAAGAGGC TGGTACCCGA 
GTTAGAAGGA AGAAGAAACA GCCAAGGAAG TTTGAGGAAC CAAAAGAGAT CAAGAAGCTT 
GAAAACACAA ATAACTTTTT AGAAAGAAAA ATGATACCCA AAAAGCAAAG AAATCAAGAC 
AAAGGCAGAA GTAACCCAGA GTTAAGTAAG TTACCATCAC CTGTGTTTGC CCAAACCATG 
AAAAGTTTGA GACTGAGTGG GGAAGAGAAG GGCCTAAAGT CCTCTGACTT GGCAGAAGAG 
GAGAAAGAGA GAAAAAATGA ACCCAAAGAA AAATACCAGA AAAGGTATGA TTTCGACAAA 
GAAGAAAAAG CCAGAAAAGA GCCAAAGGGA TTAAAGTCAT TTAAGGAAAT CAGAAATGCA 
TTTGATTTAT TTAAAAAAAC AGCAGAAGAA AAAAATGATC TTGAAAATAA TTCAAAAAGA 
GAAGAAATCT CACTGGATTA TAAAATTACA CATGATAACA AAACCAAAGA CAAGTGTTCA 
CTTAGAGAAG AGAGAAACAC TAGAGATGAG ACTGACACTT GGGCATACAT TGCTGCAGAA 
GGGGATCAGG AAGTTTCAGA CAGTGTATGC CAAACAGATG AGAGTTCTGA TGGCAAGCAA 
CCAATTTTGA GTTTGGGTAT GGACCTGCAG TTGGAGTGGA TGAAATTAGA AGATTTTCAA 
AAGCATCTTG ATGGGGAAGA TGAGCCCTTT ATCACAGCAA ATAGGATTCC CAGCAATTTG 
TTGAGGGATG CTGTGAAAAA TGGAGATTAT ATTGCTGTGA AGGTTGCACT GAACTCAAAT 
GAAGAATACA ACTTGGATCA AGAGGATTCT ACTGGGATGA CACTGGTGAT GTTGGCTGCA 
GCAGGGGGTC AGGATGACCT CCTCAGACTC CTTATCACCA AAGGAGCAAA AGTGAATGGG 
CGCCAGAAAA ACGGGACTAC AGCTCTCATT CATGCTGCTG AGAAGAACTT TCTAACGACG 
GTAGCTATTC TGTTGGAGGC TGGAGCTTTT GTCAATGTTC AGCAAAGCAA CGGTGAGACT 
GCACTCATGA AGGCATGCAA AAGAGGGAAT TCGGACATCG TCCGTCTTGT GATTGAGTGT 
GGAGCTGACT GCAACATCCT GTCAAAGCAC CAGAATAGCG CACTGTACTT TGCAAAGCAG 
TGTAATAATG TGCTTGTATA TGAACTGCTG AAGAGCCACT TGGAGACCCT TTCAAGAGTT 
GCAGAAGAAA CAATCAGGGA TTACTTTGAA TCTCGCCTTG CTCTACTGGA ACCTGTTTTC 
CCAATTGCAT GTCATCGGCT CTGTGAGGGG CCCGATTTCT CAACAGATTT CAATTACATG 
CCCCCTCAGA ACATGCCTGA AGGATCTGGT GTCCTCCTCT TCATTTTCCA TGCAAACTTC 
TTGGGTAAAG ATGTCATTGC TCGGCTTTGT GGACCATGCA GTGTGCAAGC CGTGGTGCTA 
AATGATAAAT TCCAGCTACC TGTTTTTCTG GACAGTCACT TTGTTTATTC ATTCAGCCCT 
GTTGCTGGCC CCAATAAGTT GTTTATAAGG TTGACGGAAG CACCCTTTGC CAAGGTGAAG 
TTGCTAATAG GTGCTTACAG AGTACAGCTG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017375.2
CDS	71..2626
Translation	MAAATEENMSVAALVMSVPDNIGRSPEVEGGGAAGEEKDAATKGTVAVGDSEEDGEDVFEVERILDMKCEGGKNLYKVRWKGYTSDDDTWEPEVHLEDCKEVLLEFRKKVAENKAKAVRKDIQKLSLNNDIFEADSDIDQQGDTKEDTSPRKKKKKIKYKEDKSPDDLRKKRAKMGKLKDKFKTELESTSEILGFDVKTKKRILEVKEELKDSKKPKKDEIKETKKTKRADIRDLKIKIREDVKDNRKTKKERYIDSPLESESPNDSFTLEDESEDFLSDNKEKQNVRTAKDKTGQDTVQESIFEKHLDDLISIEEAGTRVRRKKKQPRKFEEPKEIKKLENTNNFLERKMIPKKQRNQDKGRSNPELSKLPSPVFAQTMKSLRLSGEEKGLKSSDLAEEEKERKNEPKEKYQKRYDFDKEEKARKEPKGLKSFKEIRNAFDLFKKTAEEKNDLENNSKREEISLDYKITHDNKTKDKCSLREERNTRDETDTWAYIAAEGDQEVSDSVCQTDESSDGKQPILSLGMDLQLEWMKLEDFQKHLDGEDEPFITANRIPSNLLRDAVKNGDYIAVKVALNSNEEYNLDQEDSTGMTLVMLAAAGGQDDLLRLLITKGAKVNGRQKNGTTALIHAAEKNFLTTVAILLEAGAFVNVQQSNGETALMKACKRGNSDIVRLVIECGADCNILSKHQNSALYFAKQCNNVLVYELLKSHLETLSRVAEETIRDYFESRLALLEPVFPIACHRLCEGPDFSTDFNYMPPQNMPEGSGVLLFIFHANFLGKDVIARLCGPCSVQAVVLNDKFQLPVFLDSHFVYSFSPVAGPNKLFIRLTEAPFAKVKLLIGAYRVQLQ

Target ORF information:

RefSeq Version	NM_001017375.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus M-phase phosphoprotein 8 (Mphosph8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017375.2

ORF Insert Sequence:

ATGGCGGCGG CTACTGAGGA AAACATGAGT GTGGCCGCGC TAGTTATGTC AGTTCCGGAC 
AACATTGGCC GATCTCCCGA GGTGGAAGGA GGTGGAGCAG CGGGCGAAGA GAAGGACGCA 
GCCACCAAGG GAACGGTGGC GGTTGGGGAC AGCGAGGAGG ATGGGGAGGA CGTTTTCGAG 
GTGGAGAGGA TCCTGGACAT GAAGTGCGAG GGAGGTAAGA ATCTTTATAA GGTTCGATGG 
AAAGGATATA CGTCTGATGA TGACACTTGG GAACCTGAGG TTCATCTGGA GGACTGTAAA 
GAAGTTCTTC TTGAATTTAG GAAGAAAGTT GCTGAGAACA AAGCTAAAGC AGTAAGGAAA 
GATATTCAGA AACTATCTTT AAACAATGAC ATATTTGAGG CAGACAGTGA TATCGACCAG 
CAAGGTGACA CAAAAGAAGA TACGTCACCA AGGAAGAAAA AGAAAAAAAT AAAGTACAAA 
GAAGATAAAA GTCCAGATGA TCTGAGGAAG AAAAGGGCAA AGATGGGAAA ACTAAAAGAT 
AAGTTCAAGA CAGAGCTGGA GAGCACCTCT GAAATTTTAG GTTTTGATGT AAAGACAAAG 
AAAAGAATTT TGGAAGTCAA AGAAGAATTA AAGGACTCCA AAAAACCCAA AAAGGATGAA 
ATAAAGGAAA CCAAGAAAAC TAAAAGGGCT GACATAAGAG ATTTAAAGAT TAAAATAAGA 
GAAGATGTCA AAGACAACCG AAAAACAAAG AAAGAAAGAT ATATAGACTC TCCCCTGGAG 
TCCGAGTCAC CTAATGATTC CTTCACTCTA GAGGATGAGA GTGAAGACTT TCTTTCCGAC 
AACAAAGAGA AACAAAATGT AAGAACTGCA AAGGATAAGA CAGGACAGGA TACAGTGCAA 
GAGAGCATTT TTGAGAAACA TCTGGATGAC CTTATAAGTA TTGAAGAGGC TGGTACCCGA 
GTTAGAAGGA AGAAGAAACA GCCAAGGAAG TTTGAGGAAC CAAAAGAGAT CAAGAAGCTT 
GAAAACACAA ATAACTTTTT AGAAAGAAAA ATGATACCCA AAAAGCAAAG AAATCAAGAC 
AAAGGCAGAA GTAACCCAGA GTTAAGTAAG TTACCATCAC CTGTGTTTGC CCAAACCATG 
AAAAGTTTGA GACTGAGTGG GGAAGAGAAG GGCCTAAAGT CCTCTGACTT GGCAGAAGAG 
GAGAAAGAGA GAAAAAATGA ACCCAAAGAA AAATACCAGA AAAGGTATGA TTTCGACAAA 
GAAGAAAAAG CCAGAAAAGA GCCAAAGGGA TTAAAGTCAT TTAAGGAAAT CAGAAATGCA 
TTTGATTTAT TTAAAAAAAC AGCAGAAGAA AAAAATGATC TTGAAAATAA TTCAAAAAGA 
GAAGAAATCT CACTGGATTA TAAAATTACA CATGATAACA AAACCAAAGA CAAGTGTTCA 
CTTAGAGAAG AGAGAAACAC TAGAGATGAG ACTGACACTT GGGCATACAT TGCTGCAGAA 
GGGGATCAGG AAGTTTCAGA CAGTGTATGC CAAACAGATG AGAGTTCTGA TGGCAAGCAA 
CCAATTTTGA GTTTGGGTAT GGACCTGCAG TTGGAGTGGA TGAAATTAGA AGATTTTCAA 
AAGCATCTTG ATGGGGAAGA TGAGCCCTTT ATCACAGCAA ATAGGATTCC CAGCAATTTG 
TTGAGGGATG CTGTGAAAAA TGGAGATTAT ATTGCTGTGA AGGTTGCACT GAACTCAAAT 
GAAGAATACA ACTTGGATCA AGAGGATTCT ACTGGGATGA CACTGGTGAT GTTGGCTGCA 
GCAGGGGGTC AGGATGACCT CCTCAGACTC CTTATCACCA AAGGAGCAAA AGTGAATGGG 
CGCCAGAAAA ACGGGACTAC AGCTCTCATT CATGCTGCTG AGAAGAACTT TCTAACGACG 
GTAGCTATTC TGTTGGAGGC TGGAGCTTTT GTCAATGTTC AGCAAAGCAA CGGTGAGACT 
GCACTCATGA AGGCATGCAA AAGAGGGAAT TCGGACATCG TCCGTCTTGT GATTGAGTGT 
GGAGCTGACT GCAACATCCT GTCAAAGCAC CAGAATAGCG CACTGTACTT TGCAAAGCAG 
TGTAATAATG TGCTTGTATA TGAACTGCTG AAGAGCCACT TGGAGACCCT TTCAAGAGTT 
GCAGAAGAAA CAATCAGGGA TTACTTTGAA TCTCGCCTTG CTCTACTGGA ACCTGTTTTC 
CCAATTGCAT GTCATCGGCT CTGTGAGGGG CCCGATTTCT CAACAGATTT CAATTACATG 
CCCCCTCAGA ACATGCCTGA AGGATCTGGT GTCCTCCTCT TCATTTTCCA TGCAAACTTC 
TTGGGTAAAG ATGTCATTGC TCGGCTTTGT GGACCATGCA GTGTGCAAGC CGTGGTGCTA 
AATGATAAAT TCCAGCTACC TGTTTTTCTG GACAGTCACT TTGTTTATTC ATTCAGCCCT 
GTTGCTGGCC CCAATAAGTT GTTTATAAGG TTGACGGAAG CACCCTTTGC CAAGGTGAAG 
TTGCTAATAG GTGCTTACAG AGTACAGCTG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective silencing of euchromatic L1s revealed by genome-wide screens for L1 regulators.
Nature553(7687)228-232(2018 Jan)
Liu N,Lee CH,Swigut T,Grow E,Gu B,Bassik MC,Wysocka J

Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.
Nature genetics49(7)1035-1044(2017 Jul)
Tchasovnikarova IA,Timms RT,Douse CH,Roberts RC,Dougan G,Kingston RE,Modis Y,Lehner PJ

GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
Science (New York, N.Y.)348(6242)1481-1485(2015 Jun)
Tchasovnikarova IA,Timms RT,Matheson NJ,Wals K,Antrobus R,G?ttgens B,Dougan G,Dawson MA,Lehner PJ

Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.
Biochemical and biophysical research communications458(3)470-5(2015 Mar)
Murata K,Sato S,Haruta M,Goshima T,Chiba Y,Takahashi S,Sharif J,Koseki H,Nakanishi M,Shimada M

Methyl-H3K9-binding protein MPP8 mediates E-cadherin gene silencing and promotes tumour cell motility and invasion.
The EMBO journal29(21)3673-87(2010 Nov)
Kokura K,Sun L,Bedford MT,Fang J

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Mphosph8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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