Smtn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smtn
Entrez Gene ID	289734
Full Name	smoothelin
General protein information	Preferred Names smoothelin Names smoothelin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599120.1	XP_017454609.1	smoothelin isoform X3
NM_001013049.2	NP_001013067.2	smoothelin
XM_006251196.3	XP_006251258.1	smoothelin isoform X2
XM_006251194.3	XP_006251256.1	smoothelin isoform X1
XM_008770259.2	XP_008768481.1	smoothelin isoform X4
XM_006251195.3	XP_006251257.1	smoothelin isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	smtn	NP_001135603.2
Rattus norvegicus (Norway rat)	Smtn	NP_001013067.2
Canis lupus familiaris (dog)	SMTN	XP_005636519.1
Homo sapiens (human)	SMTN	NP_008863.3
Pan troglodytes (chimpanzee)	SMTN	XP_003317249.2
	SMTN	XP_002798381.1
Bos taurus (cattle)	SMTN	NP_001070347.1
Mus musculus (house mouse)	Smtn	NP_001271356.1

Gene Ontology
Bibliography

Related articles in PubMed

Smoothelin-B deficiency results in reduced arterial contractility, hypertension, and cardiac hypertrophy in mice.
Rensen SS, Niessen PM, van Deursen JM, Janssen BJ, Heijman E, Hermeling E, Meens M, Lie N, Gijbels MJ, Strijkers GJ, Doevendans PA, Hofker MH, De Mey JG, van Eys GJ
Circulation118(8)828-36(2008 Aug)

Smoothelin is an indicator of reversible phenotype modulation of smooth muscle cells in balloon-injured rat carotid arteries.
B?r H, Wende P, Watson L, Denger S, van Eys G, Kreuzer J, Jahn L
Basic research in cardiology97(1)9-16(2002 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Smoothelin is an indicator of reversible phenotype modulation of smooth muscle cells in balloon-injured rat carotid arteries.
Title: Smoothelin is an indicator of reversible phenotype modulation of smooth muscle cells in balloon-injured rat carotid arteries.

The following Smtn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smtn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa30220	NM_001013049.2 Latest version!	Rattus norvegicus smoothelin (Smtn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$538.30 $769.00
ORa36233	XM_006251194.3 Latest version!	Rattus norvegicus smoothelin (Smtn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa36233	XM_006251195.3 Latest version!	Rattus norvegicus smoothelin (Smtn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa36234	XM_006251196.3 Latest version!	Rattus norvegicus smoothelin (Smtn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa44819	XM_008770259.2 Latest version!	Rattus norvegicus smoothelin (Smtn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa58256	XM_017599120.1 Latest version!	Rattus norvegicus smoothelin (Smtn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smtn ( NM_001013049.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013067.2 Rattus norvegicus smoothelin (Smtn), mRNA.

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CloneID	ORa30220
Clone ID Related Accession (Same CDS sequence)	NM_001013049.2
Accession Version	NM_001013049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	smoothelin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07015887.1. On or before Aug 29, 2008 this sequence version replaced XM_214078.4, XM_001061663.1, XM_001066720.1, NM_001013049.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGA TGGAGCCCGA TCCAGCAGAG CCCCCCTCTG CCACAGTGGA AGCAGCTAAT 
GGAACAGAGC AGACTCTGGA CAAAGTGAAC AAAGGCCCAG AGGGGCGGAG CCCCCTGAGT 
GCAGAGGAGC TGATGGCCAT TGAGGACGAA GGAATCCTGG ACAAGATGCT GGACCAGGCT 
ACGAACTTTG AAGAGCGGAA GCTCATCCGG GCTGCGCTCC GTGAGCTCCG ACAAAGAAAG 
AGAGACCAGA GGGACAAGGA ACGAGAACGG CGGCTACGAG AGGCACGGGC CCGGCCAGGC 
GAGAGCCGAA GCAATATGGC TACTACAGAG ACCACCACCA GGCACAGCCA GAGGGCGGCT 
GATGGCTCGG CGGTCAGCAC AGTTACCAAA ACTGAGCGGC TCGTCCACTC CAATGACGGC 
ACGCAGACTG CGCGCACCAC CACAGTGGAG TCGAGTTTCG TGAGGCGCTC GGAGAATGGC 
AGCAGCAGCA GCAGCAGCAC CACGGTCCAA ACCAAGACCT TTTCCTCTTC CTCTTCCTCA 
TCCAAAAAAA TGGGCAGTAT CTTCGACCGA GAGGACCAAA CCAGCTCACG TTCTGGCAGC 
CTGGCGGCCC TCGAAAAACG CCAGGCAGAG AAGAAGAAAG AGCTCATGAA GGCACAGAGT 
CTGCCCAAGA CCTCAGCGTC CCAAGCACGC AAGGCCATGA TTGAGAAACT AGAGAAGGAA 
GGCTCTTCGG GCAGTCCTGG CACACCCCGT ACAGCGGTAC AGCGTTCTAC CAGCTTCGGA 
GTCCCCAACG CCAACAGCAT CAAGCAGATG TTGCTGGACT GGTGCCGAGC CAAGACCCGT 
GGCTACGAGC ACGTGGACAT CCAGAACTTC TCCTCCAGCT GGAGTGATGG GATGGCTTTC 
TGTGCCCTGG TGCACAATTT CTTCCCTGAG GCTTTTGACT ATGGACAGCT TAGCCCACAA 
AACCGGCGCC AGAACTTTGA AATGGCCTTC TCATCTGCTG AGACCCATGC GGACTGCCCG 
CAGCTCCTGG ATACAGAGGA CATGGTGCGG CTTCGAGAGC CTGACTGGAA GTGCGTGTAC 
ACGTACATCC AGGAGTTCTA CCGCTGTCTG GTCCAGAAGG GGCTGGTAAA AACCAAAAAG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013067.2
CDS	218..2983
Translation	MADEALAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEGALASKRFRAERQDNKENWLHSQQREAEQQAALARLAGRLESMNDVEELTTLLRSAGEYEERKLIRAAIRRVRAQEIEAATLAGRLCSRLPSSGSREDSRRQAAHTLDPGKVPEEEKQGQQTEVREPTPTPEDTSQDVTTVTLLLRAPPGGTPSSPASPDSSPTTASPEPLLEPAGAQCPAAEAPVSSEPLPHPSEAPSPEPPMSPAPPSSQGRVISKPLLGSTEPSDTVDSTRGSSNTKRADLAEPKPCQRSLSVLSPRQPTQNQEPTPLAGSSQFRRAGSVRDRVRKFTSDSPVVARLQDGPPRTALASPTPTRLPGPSLMSTTLASSSSSSNSSSPSPSDTSSHKKQRELAHSLAQLQSCPQEEGPGGRSLAPRSLENRAGGPRPCSEEPSTPPPVAVGTGEPGGSMKTTFTIEIKDGRGQASTGRVVLPTGNQRAELTLGLRAPPTLLSTSSGGKNTITHVSSPGTVTRLGSVTHVTTFSHASPGNRGGCNFKMEPDPAEPPSATVEAANGTEQTLDKVNKGPEGRSPLSAEELMAIEDEGILDKMLDQATNFEERKLIRAALRELRQRKRDQRDKERERRLREARARPGESRSNMATTETTTRHSQRAADGSAVSTVTKTERLVHSNDGTQTARTTTVESSFVRRSENGSSSSSSTTVQTKTFSSSSSSSKKMGSIFDREDQTSSRSGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGSSGSPGTPRTAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEMAFSSAETHADCPQLLDTEDMVRLREPDWKCVYTYIQEFYRCLVQKGLVKTKKS

Target ORF information:

RefSeq Version	NM_001013049.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus smoothelin (Smtn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013049.2

ORF Insert Sequence:

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGA TGGAGCCCGA TCCAGCAGAG CCCCCCTCTG CCACAGTGGA AGCAGCTAAT 
GGAACAGAGC AGACTCTGGA CAAAGTGAAC AAAGGCCCAG AGGGGCGGAG CCCCCTGAGT 
GCAGAGGAGC TGATGGCCAT TGAGGACGAA GGAATCCTGG ACAAGATGCT GGACCAGGCT 
ACGAACTTTG AAGAGCGGAA GCTCATCCGG GCTGCGCTCC GTGAGCTCCG ACAAAGAAAG 
AGAGACCAGA GGGACAAGGA ACGAGAACGG CGGCTACGAG AGGCACGGGC CCGGCCAGGC 
GAGAGCCGAA GCAATATGGC TACTACAGAG ACCACCACCA GGCACAGCCA GAGGGCGGCT 
GATGGCTCGG CGGTCAGCAC AGTTACCAAA ACTGAGCGGC TCGTCCACTC CAATGACGGC 
ACGCAGACTG CGCGCACCAC CACAGTGGAG TCGAGTTTCG TGAGGCGCTC GGAGAATGGC 
AGCAGCAGCA GCAGCAGCAC CACGGTCCAA ACCAAGACCT TTTCCTCTTC CTCTTCCTCA 
TCCAAAAAAA TGGGCAGTAT CTTCGACCGA GAGGACCAAA CCAGCTCACG TTCTGGCAGC 
CTGGCGGCCC TCGAAAAACG CCAGGCAGAG AAGAAGAAAG AGCTCATGAA GGCACAGAGT 
CTGCCCAAGA CCTCAGCGTC CCAAGCACGC AAGGCCATGA TTGAGAAACT AGAGAAGGAA 
GGCTCTTCGG GCAGTCCTGG CACACCCCGT ACAGCGGTAC AGCGTTCTAC CAGCTTCGGA 
GTCCCCAACG CCAACAGCAT CAAGCAGATG TTGCTGGACT GGTGCCGAGC CAAGACCCGT 
GGCTACGAGC ACGTGGACAT CCAGAACTTC TCCTCCAGCT GGAGTGATGG GATGGCTTTC 
TGTGCCCTGG TGCACAATTT CTTCCCTGAG GCTTTTGACT ATGGACAGCT TAGCCCACAA 
AACCGGCGCC AGAACTTTGA AATGGCCTTC TCATCTGCTG AGACCCATGC GGACTGCCCG 
CAGCTCCTGG ATACAGAGGA CATGGTGCGG CTTCGAGAGC CTGACTGGAA GTGCGTGTAC 
ACGTACATCC AGGAGTTCTA CCGCTGTCTG GTCCAGAAGG GGCTGGTAAA AACCAAAAAG 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smtn ( XM_006251194.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251256.1 Rattus norvegicus smoothelin (Smtn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa36233
Clone ID Related Accession (Same CDS sequence)	XM_006251194.3 , XM_006251195.3
Accession Version	XM_006251194.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	smoothelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251194.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGA TGGAGCCCGA TCCAGCAGAG CCCCCCTCTG CCACAGTGGA AGCAGCTAAT 
GGAACAGAGC AGACTCTGGA CAAAGTGAAC AAAGGCCCAG AGGGGCGGAG CCCCCTGAGT 
GCAGAGGAGC TGATGGCCAT TGAGGACGAA GGAATCCTGG ACAAGATGCT GGACCAGGCT 
ACGAACTTTG AAGAGCGGAA GCTCATCCGG GCTGCGCTCC GTGAGCTCCG ACAAAGAAAG 
AGAGACCAGA GGGACAAGGA ACGAGAACGG CGGCTACGAG AGGCACGGGC CCGGCCAGGC 
GAGAGCCGAA GCAATATGGC TACTACAGAG ACCACCACCA GGCACAGCCA GAGGGCGGCT 
GATGGCTCGG CGGTCAGCAC AGTTACCAAA ACTGAGCGGC TCGTCCACTC CAATGACGGC 
ACGCAGACTG CGCGCACCAC CACAGTGGAG TCGAGTTTCG TGAGGCGCTC GGAGAATGGC 
AGCAGCAGCA GCAGCAGCAC CACGGTCCAA ACCAAGACCT TTTCCTCTTC CTCTTCCTCA 
TCCAAAAAAA TGGGCAGTAT CTTCGACCGA GAGGACCAAA CCAGCTCACG TTCTGGCAGC 
CTGGCGGCCC TCGAAAAACG CCAGGCAGAG AAGAAGAAAG AGCTCATGAA GGCACAGAGT 
CTGCCCAAGA CCTCAGCGTC CCAAGCACGC AAGGCCATGA TTGAGAAACT AGAGAAGGAA 
GGCTCTTCGG GCAGTCCTGG CACACCCCGT ACAGCGGTAC AGCGTTCTAC CAGCTTCGGA 
GTCCCCAACG CCAACAGCAT CAAGCAGATG TTGCTGGACT GGTGCCGAGC CAAGACCCGT 
GGCTACGAGC ACGTGGACAT CCAGAACTTC TCCTCCAGCT GGAGTGATGG GATGGCTTTC 
TGTGCCCTGG TGCACAATTT CTTCCCTGAG GCTTTTGACT ATGGACAGCT TAGCCCACAA 
AACCGGCGCC AGAACTTTGA AATGGCCTTC TCATCTGCTG AGATGCTGGT GGACTGTGTA 
CCCTTGGTGG AGGTGGAGGA CATGATGATC ATGGGCAAAA AGCCAGACCC TAAGTGCGTC 
TTCACCTACG TGCAATCGCT GTACAACCAC CTGCGGCGCC ATGAGCTGCG CCTGCGCGGC 
AAGAATGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251256.1
CDS	226..2997
Translation	MADEALAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEGALASKRFRAERQDNKENWLHSQQREAEQQAALARLAGRLESMNDVEELTTLLRSAGEYEERKLIRAAIRRVRAQEIEAATLAGRLCSRLPSSGSREDSRRQAAHTLDPGKVPEEEKQGQQTEVREPTPTPEDTSQDVTTVTLLLRAPPGGTPSSPASPDSSPTTASPEPLLEPAGAQCPAAEAPVSSEPLPHPSEAPSPEPPMSPAPPSSQGRVISKPLLGSTEPSDTVDSTRGSSNTKRADLAEPKPCQRSLSVLSPRQPTQNQEPTPLAGSSQFRRAGSVRDRVRKFTSDSPVVARLQDGPPRTALASPTPTRLPGPSLMSTTLASSSSSSNSSSPSPSDTSSHKKQRELAHSLAQLQSCPQEEGPGGRSLAPRSLENRAGGPRPCSEEPSTPPPVAVGTGEPGGSMKTTFTIEIKDGRGQASTGRVVLPTGNQRAELTLGLRAPPTLLSTSSGGKNTITHVSSPGTVTRLGSVTHVTTFSHASPGNRGGCNFKMEPDPAEPPSATVEAANGTEQTLDKVNKGPEGRSPLSAEELMAIEDEGILDKMLDQATNFEERKLIRAALRELRQRKRDQRDKERERRLREARARPGESRSNMATTETTTRHSQRAADGSAVSTVTKTERLVHSNDGTQTARTTTVESSFVRRSENGSSSSSSTTVQTKTFSSSSSSSKKMGSIFDREDQTSSRSGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGSSGSPGTPRTAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEMAFSSAEMLVDCVPLVEVEDMMIMGKKPDPKCVFTYVQSLYNHLRRHELRLRGKNV

Target ORF information:

RefSeq Version	XM_006251194.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus smoothelin (Smtn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251194.3

ORF Insert Sequence:

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGA TGGAGCCCGA TCCAGCAGAG CCCCCCTCTG CCACAGTGGA AGCAGCTAAT 
GGAACAGAGC AGACTCTGGA CAAAGTGAAC AAAGGCCCAG AGGGGCGGAG CCCCCTGAGT 
GCAGAGGAGC TGATGGCCAT TGAGGACGAA GGAATCCTGG ACAAGATGCT GGACCAGGCT 
ACGAACTTTG AAGAGCGGAA GCTCATCCGG GCTGCGCTCC GTGAGCTCCG ACAAAGAAAG 
AGAGACCAGA GGGACAAGGA ACGAGAACGG CGGCTACGAG AGGCACGGGC CCGGCCAGGC 
GAGAGCCGAA GCAATATGGC TACTACAGAG ACCACCACCA GGCACAGCCA GAGGGCGGCT 
GATGGCTCGG CGGTCAGCAC AGTTACCAAA ACTGAGCGGC TCGTCCACTC CAATGACGGC 
ACGCAGACTG CGCGCACCAC CACAGTGGAG TCGAGTTTCG TGAGGCGCTC GGAGAATGGC 
AGCAGCAGCA GCAGCAGCAC CACGGTCCAA ACCAAGACCT TTTCCTCTTC CTCTTCCTCA 
TCCAAAAAAA TGGGCAGTAT CTTCGACCGA GAGGACCAAA CCAGCTCACG TTCTGGCAGC 
CTGGCGGCCC TCGAAAAACG CCAGGCAGAG AAGAAGAAAG AGCTCATGAA GGCACAGAGT 
CTGCCCAAGA CCTCAGCGTC CCAAGCACGC AAGGCCATGA TTGAGAAACT AGAGAAGGAA 
GGCTCTTCGG GCAGTCCTGG CACACCCCGT ACAGCGGTAC AGCGTTCTAC CAGCTTCGGA 
GTCCCCAACG CCAACAGCAT CAAGCAGATG TTGCTGGACT GGTGCCGAGC CAAGACCCGT 
GGCTACGAGC ACGTGGACAT CCAGAACTTC TCCTCCAGCT GGAGTGATGG GATGGCTTTC 
TGTGCCCTGG TGCACAATTT CTTCCCTGAG GCTTTTGACT ATGGACAGCT TAGCCCACAA 
AACCGGCGCC AGAACTTTGA AATGGCCTTC TCATCTGCTG AGATGCTGGT GGACTGTGTA 
CCCTTGGTGG AGGTGGAGGA CATGATGATC ATGGGCAAAA AGCCAGACCC TAAGTGCGTC 
TTCACCTACG TGCAATCGCT GTACAACCAC CTGCGGCGCC ATGAGCTGCG CCTGCGCGGC 
AAGAATGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smtn ( XM_006251195.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251257.1 Rattus norvegicus smoothelin (Smtn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa36233
Clone ID Related Accession (Same CDS sequence)	XM_006251194.3 , XM_006251195.3
Accession Version	XM_006251195.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	smoothelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251195.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGA TGGAGCCCGA TCCAGCAGAG CCCCCCTCTG CCACAGTGGA AGCAGCTAAT 
GGAACAGAGC AGACTCTGGA CAAAGTGAAC AAAGGCCCAG AGGGGCGGAG CCCCCTGAGT 
GCAGAGGAGC TGATGGCCAT TGAGGACGAA GGAATCCTGG ACAAGATGCT GGACCAGGCT 
ACGAACTTTG AAGAGCGGAA GCTCATCCGG GCTGCGCTCC GTGAGCTCCG ACAAAGAAAG 
AGAGACCAGA GGGACAAGGA ACGAGAACGG CGGCTACGAG AGGCACGGGC CCGGCCAGGC 
GAGAGCCGAA GCAATATGGC TACTACAGAG ACCACCACCA GGCACAGCCA GAGGGCGGCT 
GATGGCTCGG CGGTCAGCAC AGTTACCAAA ACTGAGCGGC TCGTCCACTC CAATGACGGC 
ACGCAGACTG CGCGCACCAC CACAGTGGAG TCGAGTTTCG TGAGGCGCTC GGAGAATGGC 
AGCAGCAGCA GCAGCAGCAC CACGGTCCAA ACCAAGACCT TTTCCTCTTC CTCTTCCTCA 
TCCAAAAAAA TGGGCAGTAT CTTCGACCGA GAGGACCAAA CCAGCTCACG TTCTGGCAGC 
CTGGCGGCCC TCGAAAAACG CCAGGCAGAG AAGAAGAAAG AGCTCATGAA GGCACAGAGT 
CTGCCCAAGA CCTCAGCGTC CCAAGCACGC AAGGCCATGA TTGAGAAACT AGAGAAGGAA 
GGCTCTTCGG GCAGTCCTGG CACACCCCGT ACAGCGGTAC AGCGTTCTAC CAGCTTCGGA 
GTCCCCAACG CCAACAGCAT CAAGCAGATG TTGCTGGACT GGTGCCGAGC CAAGACCCGT 
GGCTACGAGC ACGTGGACAT CCAGAACTTC TCCTCCAGCT GGAGTGATGG GATGGCTTTC 
TGTGCCCTGG TGCACAATTT CTTCCCTGAG GCTTTTGACT ATGGACAGCT TAGCCCACAA 
AACCGGCGCC AGAACTTTGA AATGGCCTTC TCATCTGCTG AGATGCTGGT GGACTGTGTA 
CCCTTGGTGG AGGTGGAGGA CATGATGATC ATGGGCAAAA AGCCAGACCC TAAGTGCGTC 
TTCACCTACG TGCAATCGCT GTACAACCAC CTGCGGCGCC ATGAGCTGCG CCTGCGCGGC 
AAGAATGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251257.1
CDS	699..3470
Translation	MADEALAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEGALASKRFRAERQDNKENWLHSQQREAEQQAALARLAGRLESMNDVEELTTLLRSAGEYEERKLIRAAIRRVRAQEIEAATLAGRLCSRLPSSGSREDSRRQAAHTLDPGKVPEEEKQGQQTEVREPTPTPEDTSQDVTTVTLLLRAPPGGTPSSPASPDSSPTTASPEPLLEPAGAQCPAAEAPVSSEPLPHPSEAPSPEPPMSPAPPSSQGRVISKPLLGSTEPSDTVDSTRGSSNTKRADLAEPKPCQRSLSVLSPRQPTQNQEPTPLAGSSQFRRAGSVRDRVRKFTSDSPVVARLQDGPPRTALASPTPTRLPGPSLMSTTLASSSSSSNSSSPSPSDTSSHKKQRELAHSLAQLQSCPQEEGPGGRSLAPRSLENRAGGPRPCSEEPSTPPPVAVGTGEPGGSMKTTFTIEIKDGRGQASTGRVVLPTGNQRAELTLGLRAPPTLLSTSSGGKNTITHVSSPGTVTRLGSVTHVTTFSHASPGNRGGCNFKMEPDPAEPPSATVEAANGTEQTLDKVNKGPEGRSPLSAEELMAIEDEGILDKMLDQATNFEERKLIRAALRELRQRKRDQRDKERERRLREARARPGESRSNMATTETTTRHSQRAADGSAVSTVTKTERLVHSNDGTQTARTTTVESSFVRRSENGSSSSSSTTVQTKTFSSSSSSSKKMGSIFDREDQTSSRSGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGSSGSPGTPRTAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEMAFSSAEMLVDCVPLVEVEDMMIMGKKPDPKCVFTYVQSLYNHLRRHELRLRGKNV

Target ORF information:

RefSeq Version	XM_006251195.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus smoothelin (Smtn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251195.3

ORF Insert Sequence:

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGA TGGAGCCCGA TCCAGCAGAG CCCCCCTCTG CCACAGTGGA AGCAGCTAAT 
GGAACAGAGC AGACTCTGGA CAAAGTGAAC AAAGGCCCAG AGGGGCGGAG CCCCCTGAGT 
GCAGAGGAGC TGATGGCCAT TGAGGACGAA GGAATCCTGG ACAAGATGCT GGACCAGGCT 
ACGAACTTTG AAGAGCGGAA GCTCATCCGG GCTGCGCTCC GTGAGCTCCG ACAAAGAAAG 
AGAGACCAGA GGGACAAGGA ACGAGAACGG CGGCTACGAG AGGCACGGGC CCGGCCAGGC 
GAGAGCCGAA GCAATATGGC TACTACAGAG ACCACCACCA GGCACAGCCA GAGGGCGGCT 
GATGGCTCGG CGGTCAGCAC AGTTACCAAA ACTGAGCGGC TCGTCCACTC CAATGACGGC 
ACGCAGACTG CGCGCACCAC CACAGTGGAG TCGAGTTTCG TGAGGCGCTC GGAGAATGGC 
AGCAGCAGCA GCAGCAGCAC CACGGTCCAA ACCAAGACCT TTTCCTCTTC CTCTTCCTCA 
TCCAAAAAAA TGGGCAGTAT CTTCGACCGA GAGGACCAAA CCAGCTCACG TTCTGGCAGC 
CTGGCGGCCC TCGAAAAACG CCAGGCAGAG AAGAAGAAAG AGCTCATGAA GGCACAGAGT 
CTGCCCAAGA CCTCAGCGTC CCAAGCACGC AAGGCCATGA TTGAGAAACT AGAGAAGGAA 
GGCTCTTCGG GCAGTCCTGG CACACCCCGT ACAGCGGTAC AGCGTTCTAC CAGCTTCGGA 
GTCCCCAACG CCAACAGCAT CAAGCAGATG TTGCTGGACT GGTGCCGAGC CAAGACCCGT 
GGCTACGAGC ACGTGGACAT CCAGAACTTC TCCTCCAGCT GGAGTGATGG GATGGCTTTC 
TGTGCCCTGG TGCACAATTT CTTCCCTGAG GCTTTTGACT ATGGACAGCT TAGCCCACAA 
AACCGGCGCC AGAACTTTGA AATGGCCTTC TCATCTGCTG AGATGCTGGT GGACTGTGTA 
CCCTTGGTGG AGGTGGAGGA CATGATGATC ATGGGCAAAA AGCCAGACCC TAAGTGCGTC 
TTCACCTACG TGCAATCGCT GTACAACCAC CTGCGGCGCC ATGAGCTGCG CCTGCGCGGC 
AAGAATGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smtn ( XM_006251196.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251258.1 Rattus norvegicus smoothelin (Smtn), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa36234
Clone ID Related Accession (Same CDS sequence)	XM_006251196.3
Accession Version	XM_006251196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	smoothelin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGA TGGAGCCCGA TCCAGCAGAG CCCCCCTCTG CCACAGTGGA AGCAGCTAAT 
GGAACAGAGC AGACTCTGGA CAAAGTGAAC AAAGGCCCAG AGGGGCGGAG CCCCCTGAGT 
GCAGAGGAGC TGATGGCCAT TGAGGACGAA GGAATCCTGG ACAAGATGCT GGACCAGGCT 
ACGAACTTTG AAGAGCGGAA GCTCATCCGG GCTGCGCTCC GTGAGCTCCG ACAAAGAAAG 
AGAGACCAGA GGGACAAGGA ACGAGAACGG CGGCTACGAG AGGCACGGGC CCGGCCAGGC 
GAGAGCCGAA GCAATATGGC TACTACAGAG ACCACCACCA GGCACAGCCA GAGGGCGGCT 
GATGGCTCGG CGGTCAGCAC AGTTACCAAA ACTGAGCGGC TCGTCCACTC CAATGACGGC 
ACGCAGACTG CGCGCACCAC CACAGTGGAG TCGAGTTTCG TGAGGCGCTC GGAGAATGGC 
AGCAGCAGCA GCAGCAGCAC CACGGTCCAA ACCAAGACCT TTTCCTCTTC CTCTTCCTCA 
TCCAAAAAAA TGGGCAGTAT CTTCGACCGA GAGGACCAAA CCAGCTCACG TTCTGGCAGC 
CTGGCGGCCC TCGAAAAACG CCAGGCAGAG AAGAAGAAAG AGCTCATGAA GGCACAGAGT 
CTGCCCAAGA CCTCAGCGTC CCAAGCACGC AAGGCCATGA TTGAGAAACT AGAGAAGGAA 
GGCTCTTCGG GTCCTGGCAC ACCCCGTACA GCGGTACAGC GTTCTACCAG CTTCGGAGTC 
CCCAACGCCA ACAGCATCAA GCAGATGTTG CTGGACTGGT GCCGAGCCAA GACCCGTGGC 
TACGAGCACG TGGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCTTTCTGT 
GCCCTGGTGC ACAATTTCTT CCCTGAGGCT TTTGACTATG GACAGCTTAG CCCACAAAAC 
CGGCGCCAGA ACTTTGAAAT GGCCTTCTCA TCTGCTGAGA TGCTGGTGGA CTGTGTACCC 
TTGGTGGAGG TGGAGGACAT GATGATCATG GGCAAAAAGC CAGACCCTAA GTGCGTCTTC 
ACCTACGTGC AATCGCTGTA CAACCACCTG CGGCGCCATG AGCTGCGCCT GCGCGGCAAG 
AATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251258.1
CDS	226..2994
Translation	MADEALAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEGALASKRFRAERQDNKENWLHSQQREAEQQAALARLAGRLESMNDVEELTTLLRSAGEYEERKLIRAAIRRVRAQEIEAATLAGRLCSRLPSSGSREDSRRQAAHTLDPGKVPEEEKQGQQTEVREPTPTPEDTSQDVTTVTLLLRAPPGGTPSSPASPDSSPTTASPEPLLEPAGAQCPAAEAPVSSEPLPHPSEAPSPEPPMSPAPPSSQGRVISKPLLGSTEPSDTVDSTRGSSNTKRADLAEPKPCQRSLSVLSPRQPTQNQEPTPLAGSSQFRRAGSVRDRVRKFTSDSPVVARLQDGPPRTALASPTPTRLPGPSLMSTTLASSSSSSNSSSPSPSDTSSHKKQRELAHSLAQLQSCPQEEGPGGRSLAPRSLENRAGGPRPCSEEPSTPPPVAVGTGEPGGSMKTTFTIEIKDGRGQASTGRVVLPTGNQRAELTLGLRAPPTLLSTSSGGKNTITHVSSPGTVTRLGSVTHVTTFSHASPGNRGGCNFKMEPDPAEPPSATVEAANGTEQTLDKVNKGPEGRSPLSAEELMAIEDEGILDKMLDQATNFEERKLIRAALRELRQRKRDQRDKERERRLREARARPGESRSNMATTETTTRHSQRAADGSAVSTVTKTERLVHSNDGTQTARTTTVESSFVRRSENGSSSSSSTTVQTKTFSSSSSSSKKMGSIFDREDQTSSRSGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGSSGPGTPRTAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEMAFSSAEMLVDCVPLVEVEDMMIMGKKPDPKCVFTYVQSLYNHLRRHELRLRGKNV

Target ORF information:

RefSeq Version	XM_006251196.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus smoothelin (Smtn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251196.3

ORF Insert Sequence:

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGA TGGAGCCCGA TCCAGCAGAG CCCCCCTCTG CCACAGTGGA AGCAGCTAAT 
GGAACAGAGC AGACTCTGGA CAAAGTGAAC AAAGGCCCAG AGGGGCGGAG CCCCCTGAGT 
GCAGAGGAGC TGATGGCCAT TGAGGACGAA GGAATCCTGG ACAAGATGCT GGACCAGGCT 
ACGAACTTTG AAGAGCGGAA GCTCATCCGG GCTGCGCTCC GTGAGCTCCG ACAAAGAAAG 
AGAGACCAGA GGGACAAGGA ACGAGAACGG CGGCTACGAG AGGCACGGGC CCGGCCAGGC 
GAGAGCCGAA GCAATATGGC TACTACAGAG ACCACCACCA GGCACAGCCA GAGGGCGGCT 
GATGGCTCGG CGGTCAGCAC AGTTACCAAA ACTGAGCGGC TCGTCCACTC CAATGACGGC 
ACGCAGACTG CGCGCACCAC CACAGTGGAG TCGAGTTTCG TGAGGCGCTC GGAGAATGGC 
AGCAGCAGCA GCAGCAGCAC CACGGTCCAA ACCAAGACCT TTTCCTCTTC CTCTTCCTCA 
TCCAAAAAAA TGGGCAGTAT CTTCGACCGA GAGGACCAAA CCAGCTCACG TTCTGGCAGC 
CTGGCGGCCC TCGAAAAACG CCAGGCAGAG AAGAAGAAAG AGCTCATGAA GGCACAGAGT 
CTGCCCAAGA CCTCAGCGTC CCAAGCACGC AAGGCCATGA TTGAGAAACT AGAGAAGGAA 
GGCTCTTCGG GTCCTGGCAC ACCCCGTACA GCGGTACAGC GTTCTACCAG CTTCGGAGTC 
CCCAACGCCA ACAGCATCAA GCAGATGTTG CTGGACTGGT GCCGAGCCAA GACCCGTGGC 
TACGAGCACG TGGACATCCA GAACTTCTCC TCCAGCTGGA GTGATGGGAT GGCTTTCTGT 
GCCCTGGTGC ACAATTTCTT CCCTGAGGCT TTTGACTATG GACAGCTTAG CCCACAAAAC 
CGGCGCCAGA ACTTTGAAAT GGCCTTCTCA TCTGCTGAGA TGCTGGTGGA CTGTGTACCC 
TTGGTGGAGG TGGAGGACAT GATGATCATG GGCAAAAAGC CAGACCCTAA GTGCGTCTTC 
ACCTACGTGC AATCGCTGTA CAACCACCTG CGGCGCCATG AGCTGCGCCT GCGCGGCAAG 
AATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smtn ( XM_008770259.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768481.1 Rattus norvegicus smoothelin (Smtn), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa44819
Clone ID Related Accession (Same CDS sequence)	XM_008770259.2
Accession Version	XM_008770259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	smoothelin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGG CAGCTGAGGA TGCTGGGACC CCCGTGGCCC ACCCGCCTGC CTTTAGCACC 
CGCCGCAGCT CTTCTGCGGG CCCCTCTCGA AGCAGCAGTC TCATGGAGCC CGATCCAGCA 
GAGCCCCCCT CTGCCACAGT GGAAGCAGCT AATGGAACAG AGCAGACTCT GGACAAAGTG 
AACAAAGGCC CAGAGGGGCG GAGCCCCCTG AGTGCAGAGG AGCTGATGGC CATTGAGGAC 
GAAGGAATCC TGGACAAGAT GCTGGACCAG GCTACGAACT TTGAAGAGCG GAAGCTCATC 
CGGGCTGCGC TCCGTGAGCT CCGACAAAGA AAGAGAGACC AGAGGGACAA GGAACGAGAA 
CGGCGGCTAC GAGAGGCACG GGCCCGGCCA GGCGAGAGCC GAAGCAATAT GGCTACTACA 
GAGACCACCA CCAGGCACAG CCAGAGGGCG GCTGATGGCT CGGCGGTCAG CACAGTTACC 
AAAACTGAGC GGCTCGTCCA CTCCAATGAC GGCACGCAGA CTGCGCGCAC CACCACAGTG 
GAGTCGAGTT TCGTGAGGCG CTCGGAGAAT GGCAGCAGCA GCAGCAGCAG CACCACGGTC 
CAAACCAAGA CCTTTTCCTC TTCCTCTTCC TCATCCAAAA AAATGGGCAG TATCTTCGAC 
CGAGAGGACC AAACCAGCTC ACGTTCTGGC AGCCTGGCGG CCCTCGAAAA ACGCCAGGCA 
GAGAAGAAGA AAGAGCTCAT GAAGGCACAG AGTCTGCCCA AGACCTCAGC GTCCCAAGCA 
CGCAAGGCCA TGATTGAGAA ACTAGAGAAG GAAGGCTCTT CGGGCAGTCC TGGCACACCC 
CGTACAGCGG TACAGCGTTC TACCAGCTTC GGAGTCCCCA ACGCCAACAG CATCAAGCAG 
ATGTTGCTGG ACTGGTGCCG AGCCAAGACC CGTGGCTACG AGCACGTGGA CATCCAGAAC 
TTCTCCTCCA GCTGGAGTGA TGGGATGGCT TTCTGTGCCC TGGTGCACAA TTTCTTCCCT 
GAGGCTTTTG ACTATGGACA GCTTAGCCCA CAAAACCGGC GCCAGAACTT TGAAATGGCC 
TTCTCATCTG CTGAGATGCT GGTGGACTGT GTACCCTTGG TGGAGGTGGA GGACATGATG 
ATCATGGGCA AAAAGCCAGA CCCTAAGTGC GTCTTCACCT ACGTGCAATC GCTGTACAAC 
CACCTGCGGC GCCATGAGCT GCGCCTGCGC GGCAAGAATG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768481.1
CDS	206..3070
Translation	MADEALAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEGALASKRFRAERQDNKENWLHSQQREAEQQAALARLAGRLESMNDVEELTTLLRSAGEYEERKLIRAAIRRVRAQEIEAATLAGRLCSRLPSSGSREDSRRQAAHTLDPGKVPEEEKQGQQTEVREPTPTPEDTSQDVTTVTLLLRAPPGGTPSSPASPDSSPTTASPEPLLEPAGAQCPAAEAPVSSEPLPHPSEAPSPEPPMSPAPPSSQGRVISKPLLGSTEPSDTVDSTRGSSNTKRADLAEPKPCQRSLSVLSPRQPTQNQEPTPLAGSSQFRRAGSVRDRVRKFTSDSPVVARLQDGPPRTALASPTPTRLPGPSLMSTTLASSSSSSNSSSPSPSDTSSHKKQRELAHSLAQLQSCPQEEGPGGRSLAPRSLENRAGGPRPCSEEPSTPPPVAVGTGEPGGSMKTTFTIEIKDGRGQASTGRVVLPTGNQRAELTLGLRAPPTLLSTSSGGKNTITHVSSPGTVTRLGSVTHVTTFSHASPGNRGGCNFKAAEDAGTPVAHPPAFSTRRSSSAGPSRSSSLMEPDPAEPPSATVEAANGTEQTLDKVNKGPEGRSPLSAEELMAIEDEGILDKMLDQATNFEERKLIRAALRELRQRKRDQRDKERERRLREARARPGESRSNMATTETTTRHSQRAADGSAVSTVTKTERLVHSNDGTQTARTTTVESSFVRRSENGSSSSSSTTVQTKTFSSSSSSSKKMGSIFDREDQTSSRSGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGSSGSPGTPRTAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEMAFSSAEMLVDCVPLVEVEDMMIMGKKPDPKCVFTYVQSLYNHLRRHELRLRGKNV

Target ORF information:

RefSeq Version	XM_008770259.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus smoothelin (Smtn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770259.2

ORF Insert Sequence:

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGAGC CAACCCCTCT TGCAGGATCA 
TCCCAGTTCC GTCGAGCTGG CTCTGTGAGA GACCGAGTGC GCAAGTTCAC ATCTGACTCT 
CCTGTGGTTG CCAGGCTCCA GGATGGCCCA CCCCGAACAG CCCTCGCTTC ACCGACCCCC 
ACAAGGCTCC CGGGCCCTTC CCTCATGAGC ACCACCCTTG CCTCCTCCTC CTCCTCCAGC 
AACTCCTCCT CTCCAAGTCC CAGTGACACT TCCTCCCACA AGAAGCAAAG AGAACTTGCT 
CATTCCCTGG CCCAGCTTCA GAGTTGCCCT CAAGAGGAGG GCCCTGGGGG GCGGAGCTTG 
GCTCCCAGGT CCCTTGAAAA CAGAGCAGGG GGGCCCAGGC CCTGCTCAGA AGAGCCCAGT 
ACCCCACCAC CCGTGGCCGT CGGCACTGGG GAGCCAGGGG GCAGTATGAA GACTACATTT 
ACCATTGAGA TCAAGGATGG CCGTGGTCAG GCCTCCACAG GCCGGGTGGT GCTGCCTACA 
GGCAACCAGA GAGCAGAACT GACTCTGGGA CTGCGGGCAC CCCCTACCCT TCTCAGCACC 
AGCAGTGGGG GCAAGAACAC CATCACCCAC GTCAGCAGCC CTGGGACTGT GACCCGACTG 
GGCAGTGTCA CTCATGTCAC TACCTTCAGC CATGCCTCCC CTGGTAACCG AGGAGGCTGC 
AACTTTAAGG CAGCTGAGGA TGCTGGGACC CCCGTGGCCC ACCCGCCTGC CTTTAGCACC 
CGCCGCAGCT CTTCTGCGGG CCCCTCTCGA AGCAGCAGTC TCATGGAGCC CGATCCAGCA 
GAGCCCCCCT CTGCCACAGT GGAAGCAGCT AATGGAACAG AGCAGACTCT GGACAAAGTG 
AACAAAGGCC CAGAGGGGCG GAGCCCCCTG AGTGCAGAGG AGCTGATGGC CATTGAGGAC 
GAAGGAATCC TGGACAAGAT GCTGGACCAG GCTACGAACT TTGAAGAGCG GAAGCTCATC 
CGGGCTGCGC TCCGTGAGCT CCGACAAAGA AAGAGAGACC AGAGGGACAA GGAACGAGAA 
CGGCGGCTAC GAGAGGCACG GGCCCGGCCA GGCGAGAGCC GAAGCAATAT GGCTACTACA 
GAGACCACCA CCAGGCACAG CCAGAGGGCG GCTGATGGCT CGGCGGTCAG CACAGTTACC 
AAAACTGAGC GGCTCGTCCA CTCCAATGAC GGCACGCAGA CTGCGCGCAC CACCACAGTG 
GAGTCGAGTT TCGTGAGGCG CTCGGAGAAT GGCAGCAGCA GCAGCAGCAG CACCACGGTC 
CAAACCAAGA CCTTTTCCTC TTCCTCTTCC TCATCCAAAA AAATGGGCAG TATCTTCGAC 
CGAGAGGACC AAACCAGCTC ACGTTCTGGC AGCCTGGCGG CCCTCGAAAA ACGCCAGGCA 
GAGAAGAAGA AAGAGCTCAT GAAGGCACAG AGTCTGCCCA AGACCTCAGC GTCCCAAGCA 
CGCAAGGCCA TGATTGAGAA ACTAGAGAAG GAAGGCTCTT CGGGCAGTCC TGGCACACCC 
CGTACAGCGG TACAGCGTTC TACCAGCTTC GGAGTCCCCA ACGCCAACAG CATCAAGCAG 
ATGTTGCTGG ACTGGTGCCG AGCCAAGACC CGTGGCTACG AGCACGTGGA CATCCAGAAC 
TTCTCCTCCA GCTGGAGTGA TGGGATGGCT TTCTGTGCCC TGGTGCACAA TTTCTTCCCT 
GAGGCTTTTG ACTATGGACA GCTTAGCCCA CAAAACCGGC GCCAGAACTT TGAAATGGCC 
TTCTCATCTG CTGAGATGCT GGTGGACTGT GTACCCTTGG TGGAGGTGGA GGACATGATG 
ATCATGGGCA AAAAGCCAGA CCCTAAGTGC GTCTTCACCT ACGTGCAATC GCTGTACAAC 
CACCTGCGGC GCCATGAGCT GCGCCTGCGC GGCAAGAATG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smtn ( XM_017599120.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454609.1 Rattus norvegicus smoothelin (Smtn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa58256
Clone ID Related Accession (Same CDS sequence)	XM_017599120.1
Accession Version	XM_017599120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	smoothelin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGGAT CATCCCAGTT CCGTCGAGCT 
GGCTCTGTGA GAGACCGAGT GCGCAAGTTC ACATCTGACT CTCCTGTGGT TGCCAGGCTC 
CAGGATGGCC CACCCCGAAC AGCCCTCGCT TCACCGACCC CCACAAGGCT CCCGGGCCCT 
TCCCTCATGA GCACCACCCT TGCCTCCTCC TCCTCCTCCA GCAACTCCTC CTCTCCAAGT 
CCCAGTGACA CTTCCTCCCA CAAGAAGCAA AGAGAACTTG CTCATTCCCT GGCCCAGCTT 
CAGAGTTGCC CTCAAGAGGA GGGCCCTGGG GGGCGGAGCT TGGCTCCCAG GTCCCTTGAA 
AACAGAGCAG GGGGGCCCAG GCCCTGCTCA GAAGAGCCCA GTACCCCACC ACCCGTGGCC 
GTCGGCACTG GGGAGCCAGG GGGCAGTATG AAGACTACAT TTACCATTGA GATCAAGGAT 
GGCCGTGGTC AGGCCTCCAC AGGCCGGGTG GTGCTGCCTA CAGGCAACCA GAGAGCAGAA 
CTGACTCTGG GACTGCGGGC ACCCCCTACC CTTCTCAGCA CCAGCAGTGG GGGCAAGAAC 
ACCATCACCC ACGTCAGCAG CCCTGGGACT GTGACCCGAC TGGGCAGTGT CACTCATGTC 
ACTACCTTCA GCCATGCCTC CCCTGGTAAC CGAGGAGGCT GCAACTTTAA GATGGAGCCC 
GATCCAGCAG AGCCCCCCTC TGCCACAGTG GAAGCAGCTA ATGGAACAGA GCAGACTCTG 
GACAAAGTGA ACAAAGGCCC AGAGGGGCGG AGCCCCCTGA GTGCAGAGGA GCTGATGGCC 
ATTGAGGACG AAGGAATCCT GGACAAGATG CTGGACCAGG CTACGAACTT TGAAGAGCGG 
AAGCTCATCC GGGCTGCGCT CCGTGAGCTC CGACAAAGAA AGAGAGACCA GAGGGACAAG 
GAACGAGAAC GGCGGCTACG AGAGGCACGG GCCCGGCCAG GCGAGAGCCG AAGCAATATG 
GCTACTACAG AGACCACCAC CAGGCACAGC CAGAGGGCGG CTGATGGCTC GGCGGTCAGC 
ACAGTTACCA AAACTGAGCG GCTCGTCCAC TCCAATGACG GCACGCAGAC TGCGCGCACC 
ACCACAGTGG AGTCGAGTTT CGTGAGGCGC TCGGAGAATG GCAGCAGCAG CAGCAGCAGC 
ACCACGGTCC AAACCAAGAC CTTTTCCTCT TCCTCTTCCT CATCCAAAAA AATGGGCAGT 
ATCTTCGACC GAGAGGACCA AACCAGCTCA CGTTCTGGCA GCCTGGCGGC CCTCGAAAAA 
CGCCAGGCAG AGAAGAAGAA AGAGCTCATG AAGGCACAGA GTCTGCCCAA GACCTCAGCG 
TCCCAAGCAC GCAAGGCCAT GATTGAGAAA CTAGAGAAGG AAGGCTCTTC GGGCAGTCCT 
GGCACACCCC GTACAGCGGT ACAGCGTTCT ACCAGCTTCG GAGTCCCCAA CGCCAACAGC 
ATCAAGCAGA TGTTGCTGGA CTGGTGCCGA GCCAAGACCC GTGGCTACGA GCACGTGGAC 
ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCTT TCTGTGCCCT GGTGCACAAT 
TTCTTCCCTG AGGCTTTTGA CTATGGACAG CTTAGCCCAC AAAACCGGCG CCAGAACTTT 
GAAATGGCCT TCTCATCTGC TGAGATGCTG GTGGACTGTG TACCCTTGGT GGAGGTGGAG 
GACATGATGA TCATGGGCAA AAAGCCAGAC CCTAAGTGCG TCTTCACCTA CGTGCAATCG 
CTGTACAACC ACCTGCGGCG CCATGAGCTG CGCCTGCGCG GCAAGAATGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454609.1
CDS	226..2979
Translation	MADEALAGLDEGALRKLLEVTADLAERRRIRSAIRELQRQELEREEGALASKRFRAERQDNKENWLHSQQREAEQQAALARLAGRLESMNDVEELTTLLRSAGEYEERKLIRAAIRRVRAQEIEAATLAGRLCSRLPSSGSREDSRRQAAHTLDPGKVPEEEKQGQQTEVREPTPTPEDTSQDVTTVTLLLRAPPGGTPSSPASPDSSPTTASPEPLLEPAGAQCPAAEAPVSSEPLPHPSEAPSPEPPMSPAPPSSQGRVISKPLLGSTEPSDTVDSTRGSSNTKRADLAEPKPCQRSLSVLSPRQPTQNQGSSQFRRAGSVRDRVRKFTSDSPVVARLQDGPPRTALASPTPTRLPGPSLMSTTLASSSSSSNSSSPSPSDTSSHKKQRELAHSLAQLQSCPQEEGPGGRSLAPRSLENRAGGPRPCSEEPSTPPPVAVGTGEPGGSMKTTFTIEIKDGRGQASTGRVVLPTGNQRAELTLGLRAPPTLLSTSSGGKNTITHVSSPGTVTRLGSVTHVTTFSHASPGNRGGCNFKMEPDPAEPPSATVEAANGTEQTLDKVNKGPEGRSPLSAEELMAIEDEGILDKMLDQATNFEERKLIRAALRELRQRKRDQRDKERERRLREARARPGESRSNMATTETTTRHSQRAADGSAVSTVTKTERLVHSNDGTQTARTTTVESSFVRRSENGSSSSSSTTVQTKTFSSSSSSSKKMGSIFDREDQTSSRSGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGSSGSPGTPRTAVQRSTSFGVPNANSIKQMLLDWCRAKTRGYEHVDIQNFSSSWSDGMAFCALVHNFFPEAFDYGQLSPQNRRQNFEMAFSSAEMLVDCVPLVEVEDMMIMGKKPDPKCVFTYVQSLYNHLRRHELRLRGKNV

Target ORF information:

RefSeq Version	XM_017599120.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus smoothelin (Smtn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599120.1

ORF Insert Sequence:

ATGGCAGATG AGGCCTTGGC TGGGCTGGAT GAAGGAGCCC TCCGAAAACT GCTGGAGGTC 
ACAGCAGATC TGGCAGAACG GCGGCGGATC CGTTCAGCCA TCCGGGAGCT GCAGCGGCAG 
GAGCTAGAAC GAGAAGAGGG GGCTCTGGCA TCCAAACGTT TCCGTGCTGA GCGGCAAGAC 
AACAAGGAGA ACTGGCTACA CTCTCAACAG CGAGAAGCCG AGCAACAGGC CGCCCTCGCA 
CGGTTGGCAG GGAGACTGGA GTCCATGAAC GATGTAGAGG AGTTGACCAC ACTGCTTCGG 
AGTGCTGGTG AATACGAGGA ACGCAAGTTG ATCCGAGCCG CCATCCGCCG TGTGCGAGCT 
CAGGAGATCG AAGCTGCCAC CTTGGCTGGG AGACTATGCA GTAGACTTCC CAGTAGTGGG 
TCCCGAGAAG ACAGCAGGAG GCAGGCAGCA CACACATTGG ACCCTGGTAA GGTGCCAGAG 
GAAGAGAAAC AGGGACAGCA GACAGAAGTA CGAGAGCCAA CTCCGACACC AGAGGACACC 
AGCCAGGATG TGACCACAGT GACACTCCTG CTGAGGGCCC CGCCTGGGGG CACGCCCAGC 
TCACCTGCCT CCCCTGACAG TTCGCCCACC ACGGCCTCTC CAGAGCCTCT GCTGGAGCCT 
GCTGGGGCCC AGTGTCCTGC TGCTGAGGCT CCAGTCAGCT CTGAACCACT TCCTCACCCT 
TCAGAAGCTC CCAGCCCTGA GCCTCCCATG TCGCCGGCAC CCCCAAGCTC TCAGGGGCGG 
GTCATCAGCA AGCCCCTGCT TGGCTCCACA GAGCCCTCTG ATACCGTGGA CTCCACCAGA 
GGCTCCTCTA ACACTAAGAG AGCAGACCTG GCTGAACCGA AACCCTGCCA ACGCTCCCTG 
TCTGTGCTCA GTCCCCGACA GCCAACCCAA AATCAAGGAT CATCCCAGTT CCGTCGAGCT 
GGCTCTGTGA GAGACCGAGT GCGCAAGTTC ACATCTGACT CTCCTGTGGT TGCCAGGCTC 
CAGGATGGCC CACCCCGAAC AGCCCTCGCT TCACCGACCC CCACAAGGCT CCCGGGCCCT 
TCCCTCATGA GCACCACCCT TGCCTCCTCC TCCTCCTCCA GCAACTCCTC CTCTCCAAGT 
CCCAGTGACA CTTCCTCCCA CAAGAAGCAA AGAGAACTTG CTCATTCCCT GGCCCAGCTT 
CAGAGTTGCC CTCAAGAGGA GGGCCCTGGG GGGCGGAGCT TGGCTCCCAG GTCCCTTGAA 
AACAGAGCAG GGGGGCCCAG GCCCTGCTCA GAAGAGCCCA GTACCCCACC ACCCGTGGCC 
GTCGGCACTG GGGAGCCAGG GGGCAGTATG AAGACTACAT TTACCATTGA GATCAAGGAT 
GGCCGTGGTC AGGCCTCCAC AGGCCGGGTG GTGCTGCCTA CAGGCAACCA GAGAGCAGAA 
CTGACTCTGG GACTGCGGGC ACCCCCTACC CTTCTCAGCA CCAGCAGTGG GGGCAAGAAC 
ACCATCACCC ACGTCAGCAG CCCTGGGACT GTGACCCGAC TGGGCAGTGT CACTCATGTC 
ACTACCTTCA GCCATGCCTC CCCTGGTAAC CGAGGAGGCT GCAACTTTAA GATGGAGCCC 
GATCCAGCAG AGCCCCCCTC TGCCACAGTG GAAGCAGCTA ATGGAACAGA GCAGACTCTG 
GACAAAGTGA ACAAAGGCCC AGAGGGGCGG AGCCCCCTGA GTGCAGAGGA GCTGATGGCC 
ATTGAGGACG AAGGAATCCT GGACAAGATG CTGGACCAGG CTACGAACTT TGAAGAGCGG 
AAGCTCATCC GGGCTGCGCT CCGTGAGCTC CGACAAAGAA AGAGAGACCA GAGGGACAAG 
GAACGAGAAC GGCGGCTACG AGAGGCACGG GCCCGGCCAG GCGAGAGCCG AAGCAATATG 
GCTACTACAG AGACCACCAC CAGGCACAGC CAGAGGGCGG CTGATGGCTC GGCGGTCAGC 
ACAGTTACCA AAACTGAGCG GCTCGTCCAC TCCAATGACG GCACGCAGAC TGCGCGCACC 
ACCACAGTGG AGTCGAGTTT CGTGAGGCGC TCGGAGAATG GCAGCAGCAG CAGCAGCAGC 
ACCACGGTCC AAACCAAGAC CTTTTCCTCT TCCTCTTCCT CATCCAAAAA AATGGGCAGT 
ATCTTCGACC GAGAGGACCA AACCAGCTCA CGTTCTGGCA GCCTGGCGGC CCTCGAAAAA 
CGCCAGGCAG AGAAGAAGAA AGAGCTCATG AAGGCACAGA GTCTGCCCAA GACCTCAGCG 
TCCCAAGCAC GCAAGGCCAT GATTGAGAAA CTAGAGAAGG AAGGCTCTTC GGGCAGTCCT 
GGCACACCCC GTACAGCGGT ACAGCGTTCT ACCAGCTTCG GAGTCCCCAA CGCCAACAGC 
ATCAAGCAGA TGTTGCTGGA CTGGTGCCGA GCCAAGACCC GTGGCTACGA GCACGTGGAC 
ATCCAGAACT TCTCCTCCAG CTGGAGTGAT GGGATGGCTT TCTGTGCCCT GGTGCACAAT 
TTCTTCCCTG AGGCTTTTGA CTATGGACAG CTTAGCCCAC AAAACCGGCG CCAGAACTTT 
GAAATGGCCT TCTCATCTGC TGAGATGCTG GTGGACTGTG TACCCTTGGT GGAGGTGGAG 
GACATGATGA TCATGGGCAA AAAGCCAGAC CCTAAGTGCG TCTTCACCTA CGTGCAATCG 
CTGTACAACC ACCTGCGGCG CCATGAGCTG CGCCTGCGCG GCAAGAATGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Smoothelin-B deficiency results in reduced arterial contractility, hypertension, and cardiac hypertrophy in mice.
Circulation118(8)828-36(2008 Aug)
Rensen SS,Niessen PM,van Deursen JM,Janssen BJ,Heijman E,Hermeling E,Meens M,Lie N,Gijbels MJ,Strijkers GJ,Doevendans PA,Hofker MH,De Mey JG,van Eys GJ

Smoothelin is an indicator of reversible phenotype modulation of smooth muscle cells in balloon-injured rat carotid arteries.
Basic research in cardiology97(1)9-16(2002 Jan)
B?r H,Wende P,Watson L,Denger S,van Eys G,Kreuzer J,Jahn L

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Smtn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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