Ncstn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncstn
Entrez Gene ID	289231
Full Name	nicastrin
General protein information	Preferred Names nicastrin Names nicastrin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q24
Summary	component of the gamma-secretase complex, which catalyzes the cleavage of amyloid precursor protein APP [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008769733.1	XP_008767955.1	nicastrin isoform X1
XM_008769734.2	XP_008767956.1	nicastrin isoform X2
XM_008769735.2	XP_008767957.1	nicastrin isoform X3
XM_006250283.1	XP_006250345.1	nicastrin isoform X4
NM_174864.3	NP_777353.1	nicastrin precursor

Pathways from BioSystems

Homologies

	NCSTN	XP_001117549.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001734	XP_321352.4
Caenorhabditis elegans (roundworm)	aph-2	NP_492712.2
Arabidopsis thaliana (thale cress)	AT3G52640	NP_974419.1
Homo sapiens (human)	NCSTN	NP_056146.1
Danio rerio (zebrafish)	ncstn	NP_001009556.1
Pan troglodytes (chimpanzee)	NCSTN	XP_003949661.1
Rattus norvegicus (Norway rat)	Ncstn	NP_777353.1
Drosophila melanogaster (fruit fly)	nct	NP_001262932.1
Bos taurus (cattle)	NCSTN	NP_001029647.1
Mus musculus (house mouse)	Ncstn	NP_067620.3
Canis lupus familiaris (dog)	NCSTN	XP_005640953.1
Gallus gallus (chicken)	NCSTN	NP_001004413.2
Oryza sativa (rice)	Os02g0736500	NP_001048054.1
Xenopus tropicalis (tropical clawed frog)	ncstn	NP_001123711.2

Gene Ontology
Bibliography

Related articles in PubMed

Vitamin D receptor is present on the neuronal plasma membrane and is co-localized with amyloid precursor protein, ADAM10 or Nicastrin.
Dursun E, Gezen-Ak D
PloS one12(11)e0188605(2017)

Loss of Nicastrin from Oligodendrocytes Results in Hypomyelination and Schizophrenia with Compulsive Behavior.
Dries DR, Zhu Y, Brooks MM, Forero DA, Adachi M, Cenik B, West JM, Han YH, Yu C, Arbella J, Nordin A, Adolfsson R, Del-Favero J, Lu QR, Callaerts P, Birnbaum SG, Yu G
The Journal of biological chemistry291(22)11647-56(2016 May)

An atomic structure of human ?-secretase.
Bai XC, Yan C, Yang G, Lu P, Ma D, Sun L, Zhou R, Scheres SHW, Shi Y
Nature525(7568)212-217(2015 Sep)

Pre-synaptic localization of the ?-secretase-inhibiting protein p24?2 in the mammalian brain.
Liu L, Fujino K, Nishimura M
Journal of neurochemistry133(3)422-31(2015 May)

An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
Kizuka Y, Kitazume S, Fujinawa R, Saito T, Iwata N, Saido TC, Nakano M, Yamaguchi Y, Hashimoto Y, Staufenbiel M, Hatsuta H, Murayama S, Manya H, Endo T, Taniguchi N
EMBO molecular medicine7(2)175-89(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrated the localization of VDR on the neuronal plasma membrane and the co-localization of VDR and APP or ADAM10 or Nicastrin and limited co-localization of VDR and PS1.
Title: Vitamin D receptor is present on the neuronal plasma membrane and is co-localized with amyloid precursor protein, ADAM10 or Nicastrin.

Down-regulation of the ATP-binding cassette transporter 2 (Abca2) reduces amyloid-beta production by altering Nicastrin maturation and intracellular localization.
Title: Down-regulation of the ATP-binding cassette transporter 2 (Abca2) reduces amyloid-? production by altering Nicastrin maturation and intracellular localization.

At the cellular level, nicastrin is localized predominantly in neuronal cell bodies at early postnatal stages, but is apparent both in cell bodies and dendrites/neuropil in all brain regions at the later stages.
Title: Cellular distribution of gamma-secretase subunit nicastrin in the developing and adult rat brains.

The amount of presenilin-1 in the nucleus and membrane fraction was more abundantly expressed in the denervated muscle fibers. In contrast, no significant difference in the nicastrin protein level was observed between normal and denervated muscle fibers.
Title: Normal distribution of presenilin-1 and nicastrin in skeletal muscle and the differential responses of these proteins after denervation.

a novel skipped isoform of nicastrin which may suggest a new possible control mechanism based on the alternative splicing and nonsense-mediated mRNA decay to regulate brain protein expression.
Title: Rat nicastrin gene: cDNA isolation, mRNA variants and expression pattern analysis.

The following Ncstn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncstn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa35628	XM_008769734.2 Latest version!	Rattus norvegicus nicastrin (Ncstn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa35629	XM_008769735.2 Latest version!	Rattus norvegicus nicastrin (Ncstn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa35629	XM_006250283.1 Latest version!	Rattus norvegicus nicastrin (Ncstn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa03780	NM_174864.3 Latest version!	Rattus norvegicus nicastrin (Ncstn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa03780	XM_008769733.1 Latest version!	Rattus norvegicus nicastrin (Ncstn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncstn ( XM_008769734.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767956.1 Rattus norvegicus nicastrin (Ncstn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35628
Clone ID Related Accession (Same CDS sequence)	XM_008769734.2
Accession Version	XM_008769734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nicastrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTGC TGGAGGGCAA GCTCTTTACC AGAGATATAA TGGAAAAGCT GAAGGGGGAA 
ACCAGTAGAA TCGCTGGTCT TGCCGTGACT CTAGCCAAGC CCAATTCAAC GTCAAGCTTC 
TCTCCTAGCG TGCAGTGCCC AAATGATGGG TTTGGTATTT ACTCCAACTC CTATGGGCCA 
GAGTTTGCTC ACTGCAAAAA AACATTGTGG AATGAACTGG GCAATGGCTT GGCTTATGAC 
GACTTCAGTT TCCCCATCTT TCTTCTTGAA GATGAGAACG AAACCAAGGT CATCAAGCAG 
TGCTATCAAG ATCACAACCT GGGTCAGAAT GGCTCTGCAC CGAGCTTCCC ACTGTGTGCT 
ATGCAGCTCT TCTCACACAT GCACGCCGTC ATCAGCACCG CCACCTGCAT GCGACGCAGC 
TTCATCCAGA GCACCTTCAG CATCAACCCA GAAATCGTCT GTGACCCCTT ATCCGACTAC 
AACGTATGGA GCATGCTTAA GCCTATAAAT ACGTCTGGGG GATTAGAACC TGATGTCAGG 
GTTGTGGTTG CAGCCACACG GCTGGATAGC CGGTCCTTCT TCTGGAATGT GGCCCCAGGG 
GCAGAAAGTG CCGTTGCCTC CTTTGTCACT CAGCTGGCCG CAGCTGAAGC TTTGCACAAG 
GCACCTGATG TGACCACCCT ACCCCGAAAT GTGATGTTTG TCTTCTTCCA GGGGGAAACT 
TTTGACTACA TTGGCAGCTC AAGGATGGTC TATGACATGG AGAACGGCAA GTTTCCTGTG 
CGGCTAGAGA ACATTGACTC CTTTGTGGAG CTGGGGCAGG TGGCCCTAAG GACGTCACTA 
GAACTCTGGA TGCACACAGA TCCTATGTCT CAGAAAAATG AATCTGTGAA GAACCAGGTG 
GAGGATCTTC TGGTCACTCT GGAGCAGAGT GGTGCTGATA CCCCTCAAGT TGTCCTGAGC 
AGACTAGTCC AGTCCCAGGC CCTTCCACCA TCATCTCTAC AACGATTTCT TCGGGCTCGA 
AACATCTCTG GTGTGGTCTT GGCTGACCAC TCTGGTTCCT TCCACAATCG GTATTACCAG 
AGCATTTATG ACACTGCTGA GAACATTAAC GTGACCTATC CTGAGTCGCA GAGCCCAGAA 
GAGGACCTCA ACTTTGTAAC AGACACTGCC AAGGCACTGG CAGATGTTGC CACAGTGCTG 
GCACGAGCTT TGTACAAGCT TGCAGGAGGA ACCAACTTCA ACAACTCCAT TCAGGCTGAT 
CCCCAGACAG TTACACGTCT GCTCTATGGG TTCCTGGTCA GAGCTAACAA CTCATGGTTT 
CAGTCTATCC TCAGACATGA TCTGAGGTCC TATTTGGATG ATGGGCCTCT TCAACACTAC 
ATCGCTGTCT CCAGCCCTAC CAACACGACT TACGTTGTGC AGTATGCCTT GGCAAACCTG 
ACTGGCAAGG TGACCAACCT CACCCAAGAG CAGTGCCAGG ATCCAAGTAA AGTCCCAAAT 
GAAAGCAAGG ATTTGTATGA ATACTCGTGG GTACAAGGCC CTTGGAATTC CAACAAGACA 
GAGCGGCTCC CACGGTGTGT GCGCTCCACA GTACGACTGG CTAGGGCCTT GTCCCCCGCC 
TTTGAGCTGA GTCAGTGGAG CTCCACAGAA TACTCTACGT GGGCCGAGAG CCGCTGGAAA 
GATATCCAAG CTCGGATATT CCTCATTGCC AGCAAAGAAC TTGAGTTCAT CACGCTGATC 
GTGGGCTTCA GCATCCTCGT CTTTTCTCTC ATCGTCACTT ATTGTATCAA CGCCAAGGCC 
GATGTCCTTT TCGTTGCTCC CCGAGAGCCA GGAGCTGTGT CTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767956.1
CDS	402..2249
Translation	MVLLEGKLFTRDIMEKLKGETSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVTLEQSGADTPQVVLSRLVQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPESQSPEEDLNFVTDTAKALADVATVLARALYKLAGGTNFNNSIQADPQTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAVSSPTNTTYVVQYALANLTGKVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPRCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSILVFSLIVTYCINAKADVLFVAPREPGAVSY

Target ORF information:

RefSeq Version	XM_008769734.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nicastrin (Ncstn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769734.2

ORF Insert Sequence:

ATGGTTCTGC TGGAGGGCAA GCTCTTTACC AGAGATATAA TGGAAAAGCT GAAGGGGGAA 
ACCAGTAGAA TCGCTGGTCT TGCCGTGACT CTAGCCAAGC CCAATTCAAC GTCAAGCTTC 
TCTCCTAGCG TGCAGTGCCC AAATGATGGG TTTGGTATTT ACTCCAACTC CTATGGGCCA 
GAGTTTGCTC ACTGCAAAAA AACATTGTGG AATGAACTGG GCAATGGCTT GGCTTATGAC 
GACTTCAGTT TCCCCATCTT TCTTCTTGAA GATGAGAACG AAACCAAGGT CATCAAGCAG 
TGCTATCAAG ATCACAACCT GGGTCAGAAT GGCTCTGCAC CGAGCTTCCC ACTGTGTGCT 
ATGCAGCTCT TCTCACACAT GCACGCCGTC ATCAGCACCG CCACCTGCAT GCGACGCAGC 
TTCATCCAGA GCACCTTCAG CATCAACCCA GAAATCGTCT GTGACCCCTT ATCCGACTAC 
AACGTATGGA GCATGCTTAA GCCTATAAAT ACGTCTGGGG GATTAGAACC TGATGTCAGG 
GTTGTGGTTG CAGCCACACG GCTGGATAGC CGGTCCTTCT TCTGGAATGT GGCCCCAGGG 
GCAGAAAGTG CCGTTGCCTC CTTTGTCACT CAGCTGGCCG CAGCTGAAGC TTTGCACAAG 
GCACCTGATG TGACCACCCT ACCCCGAAAT GTGATGTTTG TCTTCTTCCA GGGGGAAACT 
TTTGACTACA TTGGCAGCTC AAGGATGGTC TATGACATGG AGAACGGCAA GTTTCCTGTG 
CGGCTAGAGA ACATTGACTC CTTTGTGGAG CTGGGGCAGG TGGCCCTAAG GACGTCACTA 
GAACTCTGGA TGCACACAGA TCCTATGTCT CAGAAAAATG AATCTGTGAA GAACCAGGTG 
GAGGATCTTC TGGTCACTCT GGAGCAGAGT GGTGCTGATA CCCCTCAAGT TGTCCTGAGC 
AGACTAGTCC AGTCCCAGGC CCTTCCACCA TCATCTCTAC AACGATTTCT TCGGGCTCGA 
AACATCTCTG GTGTGGTCTT GGCTGACCAC TCTGGTTCCT TCCACAATCG GTATTACCAG 
AGCATTTATG ACACTGCTGA GAACATTAAC GTGACCTATC CTGAGTCGCA GAGCCCAGAA 
GAGGACCTCA ACTTTGTAAC AGACACTGCC AAGGCACTGG CAGATGTTGC CACAGTGCTG 
GCACGAGCTT TGTACAAGCT TGCAGGAGGA ACCAACTTCA ACAACTCCAT TCAGGCTGAT 
CCCCAGACAG TTACACGTCT GCTCTATGGG TTCCTGGTCA GAGCTAACAA CTCATGGTTT 
CAGTCTATCC TCAGACATGA TCTGAGGTCC TATTTGGATG ATGGGCCTCT TCAACACTAC 
ATCGCTGTCT CCAGCCCTAC CAACACGACT TACGTTGTGC AGTATGCCTT GGCAAACCTG 
ACTGGCAAGG TGACCAACCT CACCCAAGAG CAGTGCCAGG ATCCAAGTAA AGTCCCAAAT 
GAAAGCAAGG ATTTGTATGA ATACTCGTGG GTACAAGGCC CTTGGAATTC CAACAAGACA 
GAGCGGCTCC CACGGTGTGT GCGCTCCACA GTACGACTGG CTAGGGCCTT GTCCCCCGCC 
TTTGAGCTGA GTCAGTGGAG CTCCACAGAA TACTCTACGT GGGCCGAGAG CCGCTGGAAA 
GATATCCAAG CTCGGATATT CCTCATTGCC AGCAAAGAAC TTGAGTTCAT CACGCTGATC 
GTGGGCTTCA GCATCCTCGT CTTTTCTCTC ATCGTCACTT ATTGTATCAA CGCCAAGGCC 
GATGTCCTTT TCGTTGCTCC CCGAGAGCCA GGAGCTGTGT CTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncstn ( XM_008769735.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767957.1 Rattus norvegicus nicastrin (Ncstn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa35629
Clone ID Related Accession (Same CDS sequence)	XM_008769735.2 , XM_006250283.1
Accession Version	XM_008769735.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nicastrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGC TGAAGGGGGA AACCAGTAGA ATCGCTGGTC TTGCCGTGAC TCTAGCCAAG 
CCCAATTCAA CGTCAAGCTT CTCTCCTAGC GTGCAGTGCC CAAATGATGG GTTTGGTATT 
TACTCCAACT CCTATGGGCC AGAGTTTGCT CACTGCAAAA AAACATTGTG GAATGAACTG 
GGCAATGGCT TGGCTTATGA CGACTTCAGT TTCCCCATCT TTCTTCTTGA AGATGAGAAC 
GAAACCAAGG TCATCAAGCA GTGCTATCAA GATCACAACC TGGGTCAGAA TGGCTCTGCA 
CCGAGCTTCC CACTGTGTGC TATGCAGCTC TTCTCACACA TGCACGCCGT CATCAGCACC 
GCCACCTGCA TGCGACGCAG CTTCATCCAG AGCACCTTCA GCATCAACCC AGAAATCGTC 
TGTGACCCCT TATCCGACTA CAACGTATGG AGCATGCTTA AGCCTATAAA TACGTCTGGG 
GGATTAGAAC CTGATGTCAG GGTTGTGGTT GCAGCCACAC GGCTGGATAG CCGGTCCTTC 
TTCTGGAATG TGGCCCCAGG GGCAGAAAGT GCCGTTGCCT CCTTTGTCAC TCAGCTGGCC 
GCAGCTGAAG CTTTGCACAA GGCACCTGAT GTGACCACCC TACCCCGAAA TGTGATGTTT 
GTCTTCTTCC AGGGGGAAAC TTTTGACTAC ATTGGCAGCT CAAGGATGGT CTATGACATG 
GAGAACGGCA AGTTTCCTGT GCGGCTAGAG AACATTGACT CCTTTGTGGA GCTGGGGCAG 
GTGGCCCTAA GGACGTCACT AGAACTCTGG ATGCACACAG ATCCTATGTC TCAGAAAAAT 
GAATCTGTGA AGAACCAGGT GGAGGATCTT CTGGTCACTC TGGAGCAGAG TGGTGCTGAT 
ACCCCTCAAG TTGTCCTGAG CAGACTAGTC CAGTCCCAGG CCCTTCCACC ATCATCTCTA 
CAACGATTTC TTCGGGCTCG AAACATCTCT GGTGTGGTCT TGGCTGACCA CTCTGGTTCC 
TTCCACAATC GGTATTACCA GAGCATTTAT GACACTGCTG AGAACATTAA CGTGACCTAT 
CCTGAGTCGC AGAGCCCAGA AGAGGACCTC AACTTTGTAA CAGACACTGC CAAGGCACTG 
GCAGATGTTG CCACAGTGCT GGCACGAGCT TTGTACAAGC TTGCAGGAGG AACCAACTTC 
AACAACTCCA TTCAGGCTGA TCCCCAGACA GTTACACGTC TGCTCTATGG GTTCCTGGTC 
AGAGCTAACA ACTCATGGTT TCAGTCTATC CTCAGACATG ATCTGAGGTC CTATTTGGAT 
GATGGGCCTC TTCAACACTA CATCGCTGTC TCCAGCCCTA CCAACACGAC TTACGTTGTG 
CAGTATGCCT TGGCAAACCT GACTGGCAAG GTGACCAACC TCACCCAAGA GCAGTGCCAG 
GATCCAAGTA AAGTCCCAAA TGAAAGCAAG GATTTGTATG AATACTCGTG GGTACAAGGC 
CCTTGGAATT CCAACAAGAC AGAGCGGCTC CCACGGTGTG TGCGCTCCAC AGTACGACTG 
GCTAGGGCCT TGTCCCCCGC CTTTGAGCTG AGTCAGTGGA GCTCCACAGA ATACTCTACG 
TGGGCCGAGA GCCGCTGGAA AGATATCCAA GCTCGGATAT TCCTCATTGC CAGCAAAGAA 
CTTGAGTTCA TCACGCTGAT CGTGGGCTTC AGCATCCTCG TCTTTTCTCT CATCGTCACT 
TATTGTATCA ACGCCAAGGC CGATGTCCTT TTCGTTGCTC CCCGAGAGCC AGGAGCTGTG 
TCTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767957.1
CDS	202..2010
Translation	MEKLKGETSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVTLEQSGADTPQVVLSRLVQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPESQSPEEDLNFVTDTAKALADVATVLARALYKLAGGTNFNNSIQADPQTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAVSSPTNTTYVVQYALANLTGKVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPRCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSILVFSLIVTYCINAKADVLFVAPREPGAVSY

Target ORF information:

RefSeq Version	XM_008769735.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nicastrin (Ncstn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769735.2

ORF Insert Sequence:

ATGGAAAAGC TGAAGGGGGA AACCAGTAGA ATCGCTGGTC TTGCCGTGAC TCTAGCCAAG 
CCCAATTCAA CGTCAAGCTT CTCTCCTAGC GTGCAGTGCC CAAATGATGG GTTTGGTATT 
TACTCCAACT CCTATGGGCC AGAGTTTGCT CACTGCAAAA AAACATTGTG GAATGAACTG 
GGCAATGGCT TGGCTTATGA CGACTTCAGT TTCCCCATCT TTCTTCTTGA AGATGAGAAC 
GAAACCAAGG TCATCAAGCA GTGCTATCAA GATCACAACC TGGGTCAGAA TGGCTCTGCA 
CCGAGCTTCC CACTGTGTGC TATGCAGCTC TTCTCACACA TGCACGCCGT CATCAGCACC 
GCCACCTGCA TGCGACGCAG CTTCATCCAG AGCACCTTCA GCATCAACCC AGAAATCGTC 
TGTGACCCCT TATCCGACTA CAACGTATGG AGCATGCTTA AGCCTATAAA TACGTCTGGG 
GGATTAGAAC CTGATGTCAG GGTTGTGGTT GCAGCCACAC GGCTGGATAG CCGGTCCTTC 
TTCTGGAATG TGGCCCCAGG GGCAGAAAGT GCCGTTGCCT CCTTTGTCAC TCAGCTGGCC 
GCAGCTGAAG CTTTGCACAA GGCACCTGAT GTGACCACCC TACCCCGAAA TGTGATGTTT 
GTCTTCTTCC AGGGGGAAAC TTTTGACTAC ATTGGCAGCT CAAGGATGGT CTATGACATG 
GAGAACGGCA AGTTTCCTGT GCGGCTAGAG AACATTGACT CCTTTGTGGA GCTGGGGCAG 
GTGGCCCTAA GGACGTCACT AGAACTCTGG ATGCACACAG ATCCTATGTC TCAGAAAAAT 
GAATCTGTGA AGAACCAGGT GGAGGATCTT CTGGTCACTC TGGAGCAGAG TGGTGCTGAT 
ACCCCTCAAG TTGTCCTGAG CAGACTAGTC CAGTCCCAGG CCCTTCCACC ATCATCTCTA 
CAACGATTTC TTCGGGCTCG AAACATCTCT GGTGTGGTCT TGGCTGACCA CTCTGGTTCC 
TTCCACAATC GGTATTACCA GAGCATTTAT GACACTGCTG AGAACATTAA CGTGACCTAT 
CCTGAGTCGC AGAGCCCAGA AGAGGACCTC AACTTTGTAA CAGACACTGC CAAGGCACTG 
GCAGATGTTG CCACAGTGCT GGCACGAGCT TTGTACAAGC TTGCAGGAGG AACCAACTTC 
AACAACTCCA TTCAGGCTGA TCCCCAGACA GTTACACGTC TGCTCTATGG GTTCCTGGTC 
AGAGCTAACA ACTCATGGTT TCAGTCTATC CTCAGACATG ATCTGAGGTC CTATTTGGAT 
GATGGGCCTC TTCAACACTA CATCGCTGTC TCCAGCCCTA CCAACACGAC TTACGTTGTG 
CAGTATGCCT TGGCAAACCT GACTGGCAAG GTGACCAACC TCACCCAAGA GCAGTGCCAG 
GATCCAAGTA AAGTCCCAAA TGAAAGCAAG GATTTGTATG AATACTCGTG GGTACAAGGC 
CCTTGGAATT CCAACAAGAC AGAGCGGCTC CCACGGTGTG TGCGCTCCAC AGTACGACTG 
GCTAGGGCCT TGTCCCCCGC CTTTGAGCTG AGTCAGTGGA GCTCCACAGA ATACTCTACG 
TGGGCCGAGA GCCGCTGGAA AGATATCCAA GCTCGGATAT TCCTCATTGC CAGCAAAGAA 
CTTGAGTTCA TCACGCTGAT CGTGGGCTTC AGCATCCTCG TCTTTTCTCT CATCGTCACT 
TATTGTATCA ACGCCAAGGC CGATGTCCTT TTCGTTGCTC CCCGAGAGCC AGGAGCTGTG 
TCTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncstn ( XM_006250283.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250345.1 Rattus norvegicus nicastrin (Ncstn), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa35629
Clone ID Related Accession (Same CDS sequence)	XM_008769735.2 , XM_006250283.1
Accession Version	XM_006250283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nicastrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAGC TGAAGGGGGA AACCAGTAGA ATCGCTGGTC TTGCCGTGAC TCTAGCCAAG 
CCCAATTCAA CGTCAAGCTT CTCTCCTAGC GTGCAGTGCC CAAATGATGG GTTTGGTATT 
TACTCCAACT CCTATGGGCC AGAGTTTGCT CACTGCAAAA AAACATTGTG GAATGAACTG 
GGCAATGGCT TGGCTTATGA CGACTTCAGT TTCCCCATCT TTCTTCTTGA AGATGAGAAC 
GAAACCAAGG TCATCAAGCA GTGCTATCAA GATCACAACC TGGGTCAGAA TGGCTCTGCA 
CCGAGCTTCC CACTGTGTGC TATGCAGCTC TTCTCACACA TGCACGCCGT CATCAGCACC 
GCCACCTGCA TGCGACGCAG CTTCATCCAG AGCACCTTCA GCATCAACCC AGAAATCGTC 
TGTGACCCCT TATCCGACTA CAACGTATGG AGCATGCTTA AGCCTATAAA TACGTCTGGG 
GGATTAGAAC CTGATGTCAG GGTTGTGGTT GCAGCCACAC GGCTGGATAG CCGGTCCTTC 
TTCTGGAATG TGGCCCCAGG GGCAGAAAGT GCCGTTGCCT CCTTTGTCAC TCAGCTGGCC 
GCAGCTGAAG CTTTGCACAA GGCACCTGAT GTGACCACCC TACCCCGAAA TGTGATGTTT 
GTCTTCTTCC AGGGGGAAAC TTTTGACTAC ATTGGCAGCT CAAGGATGGT CTATGACATG 
GAGAACGGCA AGTTTCCTGT GCGGCTAGAG AACATTGACT CCTTTGTGGA GCTGGGGCAG 
GTGGCCCTAA GGACGTCACT AGAACTCTGG ATGCACACAG ATCCTATGTC TCAGAAAAAT 
GAATCTGTGA AGAACCAGGT GGAGGATCTT CTGGTCACTC TGGAGCAGAG TGGTGCTGAT 
ACCCCTCAAG TTGTCCTGAG CAGACTAGTC CAGTCCCAGG CCCTTCCACC ATCATCTCTA 
CAACGATTTC TTCGGGCTCG AAACATCTCT GGTGTGGTCT TGGCTGACCA CTCTGGTTCC 
TTCCACAATC GGTATTACCA GAGCATTTAT GACACTGCTG AGAACATTAA CGTGACCTAT 
CCTGAGTCGC AGAGCCCAGA AGAGGACCTC AACTTTGTAA CAGACACTGC CAAGGCACTG 
GCAGATGTTG CCACAGTGCT GGCACGAGCT TTGTACAAGC TTGCAGGAGG AACCAACTTC 
AACAACTCCA TTCAGGCTGA TCCCCAGACA GTTACACGTC TGCTCTATGG GTTCCTGGTC 
AGAGCTAACA ACTCATGGTT TCAGTCTATC CTCAGACATG ATCTGAGGTC CTATTTGGAT 
GATGGGCCTC TTCAACACTA CATCGCTGTC TCCAGCCCTA CCAACACGAC TTACGTTGTG 
CAGTATGCCT TGGCAAACCT GACTGGCAAG GTGACCAACC TCACCCAAGA GCAGTGCCAG 
GATCCAAGTA AAGTCCCAAA TGAAAGCAAG GATTTGTATG AATACTCGTG GGTACAAGGC 
CCTTGGAATT CCAACAAGAC AGAGCGGCTC CCACGGTGTG TGCGCTCCAC AGTACGACTG 
GCTAGGGCCT TGTCCCCCGC CTTTGAGCTG AGTCAGTGGA GCTCCACAGA ATACTCTACG 
TGGGCCGAGA GCCGCTGGAA AGATATCCAA GCTCGGATAT TCCTCATTGC CAGCAAAGAA 
CTTGAGTTCA TCACGCTGAT CGTGGGCTTC AGCATCCTCG TCTTTTCTCT CATCGTCACT 
TATTGTATCA ACGCCAAGGC CGATGTCCTT TTCGTTGCTC CCCGAGAGCC AGGAGCTGTG 
TCTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250345.1
CDS	266..2074
Translation	MEKLKGETSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVTLEQSGADTPQVVLSRLVQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPESQSPEEDLNFVTDTAKALADVATVLARALYKLAGGTNFNNSIQADPQTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAVSSPTNTTYVVQYALANLTGKVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPRCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSILVFSLIVTYCINAKADVLFVAPREPGAVSY

Target ORF information:

RefSeq Version	XM_006250283.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nicastrin (Ncstn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250283.1

ORF Insert Sequence:

ATGGAAAAGC TGAAGGGGGA AACCAGTAGA ATCGCTGGTC TTGCCGTGAC TCTAGCCAAG 
CCCAATTCAA CGTCAAGCTT CTCTCCTAGC GTGCAGTGCC CAAATGATGG GTTTGGTATT 
TACTCCAACT CCTATGGGCC AGAGTTTGCT CACTGCAAAA AAACATTGTG GAATGAACTG 
GGCAATGGCT TGGCTTATGA CGACTTCAGT TTCCCCATCT TTCTTCTTGA AGATGAGAAC 
GAAACCAAGG TCATCAAGCA GTGCTATCAA GATCACAACC TGGGTCAGAA TGGCTCTGCA 
CCGAGCTTCC CACTGTGTGC TATGCAGCTC TTCTCACACA TGCACGCCGT CATCAGCACC 
GCCACCTGCA TGCGACGCAG CTTCATCCAG AGCACCTTCA GCATCAACCC AGAAATCGTC 
TGTGACCCCT TATCCGACTA CAACGTATGG AGCATGCTTA AGCCTATAAA TACGTCTGGG 
GGATTAGAAC CTGATGTCAG GGTTGTGGTT GCAGCCACAC GGCTGGATAG CCGGTCCTTC 
TTCTGGAATG TGGCCCCAGG GGCAGAAAGT GCCGTTGCCT CCTTTGTCAC TCAGCTGGCC 
GCAGCTGAAG CTTTGCACAA GGCACCTGAT GTGACCACCC TACCCCGAAA TGTGATGTTT 
GTCTTCTTCC AGGGGGAAAC TTTTGACTAC ATTGGCAGCT CAAGGATGGT CTATGACATG 
GAGAACGGCA AGTTTCCTGT GCGGCTAGAG AACATTGACT CCTTTGTGGA GCTGGGGCAG 
GTGGCCCTAA GGACGTCACT AGAACTCTGG ATGCACACAG ATCCTATGTC TCAGAAAAAT 
GAATCTGTGA AGAACCAGGT GGAGGATCTT CTGGTCACTC TGGAGCAGAG TGGTGCTGAT 
ACCCCTCAAG TTGTCCTGAG CAGACTAGTC CAGTCCCAGG CCCTTCCACC ATCATCTCTA 
CAACGATTTC TTCGGGCTCG AAACATCTCT GGTGTGGTCT TGGCTGACCA CTCTGGTTCC 
TTCCACAATC GGTATTACCA GAGCATTTAT GACACTGCTG AGAACATTAA CGTGACCTAT 
CCTGAGTCGC AGAGCCCAGA AGAGGACCTC AACTTTGTAA CAGACACTGC CAAGGCACTG 
GCAGATGTTG CCACAGTGCT GGCACGAGCT TTGTACAAGC TTGCAGGAGG AACCAACTTC 
AACAACTCCA TTCAGGCTGA TCCCCAGACA GTTACACGTC TGCTCTATGG GTTCCTGGTC 
AGAGCTAACA ACTCATGGTT TCAGTCTATC CTCAGACATG ATCTGAGGTC CTATTTGGAT 
GATGGGCCTC TTCAACACTA CATCGCTGTC TCCAGCCCTA CCAACACGAC TTACGTTGTG 
CAGTATGCCT TGGCAAACCT GACTGGCAAG GTGACCAACC TCACCCAAGA GCAGTGCCAG 
GATCCAAGTA AAGTCCCAAA TGAAAGCAAG GATTTGTATG AATACTCGTG GGTACAAGGC 
CCTTGGAATT CCAACAAGAC AGAGCGGCTC CCACGGTGTG TGCGCTCCAC AGTACGACTG 
GCTAGGGCCT TGTCCCCCGC CTTTGAGCTG AGTCAGTGGA GCTCCACAGA ATACTCTACG 
TGGGCCGAGA GCCGCTGGAA AGATATCCAA GCTCGGATAT TCCTCATTGC CAGCAAAGAA 
CTTGAGTTCA TCACGCTGAT CGTGGGCTTC AGCATCCTCG TCTTTTCTCT CATCGTCACT 
TATTGTATCA ACGCCAAGGC CGATGTCCTT TTCGTTGCTC CCCGAGAGCC AGGAGCTGTG 
TCTTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncstn ( NM_174864.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_777353.1 Rattus norvegicus nicastrin (Ncstn), mRNA.

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CloneID	ORa03780
Clone ID Related Accession (Same CDS sequence)	XM_008769733.1 , NM_174864.3
Accession Version	NM_174864.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	nicastrin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC074006.1 and AY101378.1. On Oct 8, 2005 this sequence version replaced NM_174864.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY101378.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTACGG CTAGGGGCGG CTCTGGGCCT GACCCAGGAA GTCGGGGTCT CCTTCTTCTG 
TCTTTTTCCG TGGTACTAGC AGGATTGTGT GGGGGAAACT CAGTGGAGAG GAAGATCTAC 
ATTCCCTTAA ATAAAACAGC TCCTTGTGTC CGCCTGCTCA ATGCCACTCA TCAGATTGGC 
TGCCAGTCTT CAATTAGTGG GGATACAGGG GTTATCCACG TAGTGGAGAA AGAAGACGAC 
CTGAAATGGG TATTGACGGA TGGCCCCAAC CCCCCTTACA TGGTTCTGCT GGAGGGCAAG 
CTCTTTACCA GAGATATAAT GGAAAAGCTG AAGGGGGAAA CCAGTAGAAT CGCTGGTCTT 
GCCGTGACTC TAGCCAAGCC CAATTCAACG TCAAGCTTCT CTCCTAGCGT GCAGTGCCCA 
AATGATGGGT TTGGTATTTA CTCCAACTCC TATGGGCCAG AGTTTGCTCA CTGCAAAAAA 
ACATTGTGGA ATGAACTGGG CAATGGCTTG GCTTATGACG ACTTCAGTTT CCCCATCTTT 
CTTCTTGAAG ATGAGAACGA AACCAAGGTC ATCAAGCAGT GCTATCAAGA TCACAACCTG 
GGTCAGAATG GCTCTGCACC GAGCTTCCCA CTGTGTGCTA TGCAGCTCTT CTCACACATG 
CACGCCGTCA TCAGCACCGC CACCTGCATG CGACGCAGCT TCATCCAGAG CACCTTCAGC 
ATCAACCCAG AAATCGTCTG TGACCCCTTA TCCGACTACA ACGTATGGAG CATGCTTAAG 
CCTATAAATA CGTCTGGGGG ATTAGAACCT GATGTCAGGG TTGTGGTTGC AGCCACACGG 
CTGGATAGCC GGTCCTTCTT CTGGAATGTG GCCCCAGGGG CAGAAAGTGC CGTTGCCTCC 
TTTGTCACTC AGCTGGCCGC AGCTGAAGCT TTGCACAAGG CACCTGATGT GACCACCCTA 
CCCCGAAATG TGATGTTTGT CTTCTTCCAG GGGGAAACTT TTGACTACAT TGGCAGCTCA 
AGGATGGTCT ATGACATGGA GAACGGCAAG TTTCCTGTGC GGCTAGAGAA CATTGACTCC 
TTTGTGGAGC TGGGGCAGGT GGCCCTAAGG ACGTCACTAG AACTCTGGAT GCACACAGAT 
CCTATGTCTC AGAAAAATGA ATCTGTGAAG AACCAGGTGG AGGATCTTCT GGTCACTCTG 
GAGCAGAGTG GTGCTGATAC CCCTCAAGTT GTCCTGAGCA GACTAGTCCA GTCCCAGGCC 
CTTCCACCAT CATCTCTACA ACGATTTCTT CGGGCTCGAA ACATCTCTGG TGTGGTCTTG 
GCTGACCACT CTGGTTCCTT CCACAATCGG TATTACCAGA GCATTTATGA CACTGCTGAG 
AACATTAACG TGACCTATCC TGAGTCGCAG AGCCCAGAAG AGGACCTCAA CTTTGTAACA 
GACACTGCCA AGGCACTGGC AGATGTTGCC ACAGTGCTGG CACGAGCTTT GTACAAGCTT 
GCAGGAGGAA CCAACTTCAA CAACTCCATT CAGGCTGATC CCCAGACAGT TACACGTCTG 
CTCTATGGGT TCCTGGTCAG AGCTAACAAC TCATGGTTTC AGTCTATCCT CAGACATGAT 
CTGAGGTCCT ATTTGGATGA TGGGCCTCTT CAACACTACA TCGCTGTCTC CAGCCCTACC 
AACACGACTT ACGTTGTGCA GTATGCCTTG GCAAACCTGA CTGGCAAGGT GACCAACCTC 
ACCCAAGAGC AGTGCCAGGA TCCAAGTAAA GTCCCAAATG AAAGCAAGGA TTTGTATGAA 
TACTCGTGGG TACAAGGCCC TTGGAATTCC AACAAGACAG AGCGGCTCCC ACGGTGTGTG 
CGCTCCACAG TACGACTGGC TAGGGCCTTG TCCCCCGCCT TTGAGCTGAG TCAGTGGAGC 
TCCACAGAAT ACTCTACGTG GGCCGAGAGC CGCTGGAAAG ATATCCAAGC TCGGATATTC 
CTCATTGCCA GCAAAGAACT TGAGTTCATC ACGCTGATCG TGGGCTTCAG CATCCTCGTC 
TTTTCTCTCA TCGTCACTTA TTGTATCAAC GCCAAGGCCG ATGTCCTTTT CGTTGCTCCC 
CGAGAGCCAG GAGCTGTGTC TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777353.1
CDS	93..2219
Translation	MATARGGSGPDPGSRGLLLLSFSVVLAGLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEDDLKWVLTDGPNPPYMVLLEGKLFTRDIMEKLKGETSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVTLEQSGADTPQVVLSRLVQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPESQSPEEDLNFVTDTAKALADVATVLARALYKLAGGTNFNNSIQADPQTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAVSSPTNTTYVVQYALANLTGKVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPRCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSILVFSLIVTYCINAKADVLFVAPREPGAVSY

Target ORF information:

RefSeq Version	NM_174864.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nicastrin (Ncstn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174864.3

ORF Insert Sequence:

ATGGCTACGG CTAGGGGCGG CTCTGGGCCT GACCCAGGAA GTCGGGGTCT CCTTCTTCTG 
TCTTTTTCCG TGGTACTAGC AGGATTGTGT GGGGGAAACT CAGTGGAGAG GAAGATCTAC 
ATTCCCTTAA ATAAAACAGC TCCTTGTGTC CGCCTGCTCA ATGCCACTCA TCAGATTGGC 
TGCCAGTCTT CAATTAGTGG GGATACAGGG GTTATCCACG TAGTGGAGAA AGAAGACGAC 
CTGAAATGGG TATTGACGGA TGGCCCCAAC CCCCCTTACA TGGTTCTGCT GGAGGGCAAG 
CTCTTTACCA GAGATATAAT GGAAAAGCTG AAGGGGGAAA CCAGTAGAAT CGCTGGTCTT 
GCCGTGACTC TAGCCAAGCC CAATTCAACG TCAAGCTTCT CTCCTAGCGT GCAGTGCCCA 
AATGATGGGT TTGGTATTTA CTCCAACTCC TATGGGCCAG AGTTTGCTCA CTGCAAAAAA 
ACATTGTGGA ATGAACTGGG CAATGGCTTG GCTTATGACG ACTTCAGTTT CCCCATCTTT 
CTTCTTGAAG ATGAGAACGA AACCAAGGTC ATCAAGCAGT GCTATCAAGA TCACAACCTG 
GGTCAGAATG GCTCTGCACC GAGCTTCCCA CTGTGTGCTA TGCAGCTCTT CTCACACATG 
CACGCCGTCA TCAGCACCGC CACCTGCATG CGACGCAGCT TCATCCAGAG CACCTTCAGC 
ATCAACCCAG AAATCGTCTG TGACCCCTTA TCCGACTACA ACGTATGGAG CATGCTTAAG 
CCTATAAATA CGTCTGGGGG ATTAGAACCT GATGTCAGGG TTGTGGTTGC AGCCACACGG 
CTGGATAGCC GGTCCTTCTT CTGGAATGTG GCCCCAGGGG CAGAAAGTGC CGTTGCCTCC 
TTTGTCACTC AGCTGGCCGC AGCTGAAGCT TTGCACAAGG CACCTGATGT GACCACCCTA 
CCCCGAAATG TGATGTTTGT CTTCTTCCAG GGGGAAACTT TTGACTACAT TGGCAGCTCA 
AGGATGGTCT ATGACATGGA GAACGGCAAG TTTCCTGTGC GGCTAGAGAA CATTGACTCC 
TTTGTGGAGC TGGGGCAGGT GGCCCTAAGG ACGTCACTAG AACTCTGGAT GCACACAGAT 
CCTATGTCTC AGAAAAATGA ATCTGTGAAG AACCAGGTGG AGGATCTTCT GGTCACTCTG 
GAGCAGAGTG GTGCTGATAC CCCTCAAGTT GTCCTGAGCA GACTAGTCCA GTCCCAGGCC 
CTTCCACCAT CATCTCTACA ACGATTTCTT CGGGCTCGAA ACATCTCTGG TGTGGTCTTG 
GCTGACCACT CTGGTTCCTT CCACAATCGG TATTACCAGA GCATTTATGA CACTGCTGAG 
AACATTAACG TGACCTATCC TGAGTCGCAG AGCCCAGAAG AGGACCTCAA CTTTGTAACA 
GACACTGCCA AGGCACTGGC AGATGTTGCC ACAGTGCTGG CACGAGCTTT GTACAAGCTT 
GCAGGAGGAA CCAACTTCAA CAACTCCATT CAGGCTGATC CCCAGACAGT TACACGTCTG 
CTCTATGGGT TCCTGGTCAG AGCTAACAAC TCATGGTTTC AGTCTATCCT CAGACATGAT 
CTGAGGTCCT ATTTGGATGA TGGGCCTCTT CAACACTACA TCGCTGTCTC CAGCCCTACC 
AACACGACTT ACGTTGTGCA GTATGCCTTG GCAAACCTGA CTGGCAAGGT GACCAACCTC 
ACCCAAGAGC AGTGCCAGGA TCCAAGTAAA GTCCCAAATG AAAGCAAGGA TTTGTATGAA 
TACTCGTGGG TACAAGGCCC TTGGAATTCC AACAAGACAG AGCGGCTCCC ACGGTGTGTG 
CGCTCCACAG TACGACTGGC TAGGGCCTTG TCCCCCGCCT TTGAGCTGAG TCAGTGGAGC 
TCCACAGAAT ACTCTACGTG GGCCGAGAGC CGCTGGAAAG ATATCCAAGC TCGGATATTC 
CTCATTGCCA GCAAAGAACT TGAGTTCATC ACGCTGATCG TGGGCTTCAG CATCCTCGTC 
TTTTCTCTCA TCGTCACTTA TTGTATCAAC GCCAAGGCCG ATGTCCTTTT CGTTGCTCCC 
CGAGAGCCAG GAGCTGTGTC TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ncstn ( XM_008769733.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767955.1 Rattus norvegicus nicastrin (Ncstn), transcript variant X1, mRNA.

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CloneID	ORa03780
Clone ID Related Accession (Same CDS sequence)	XM_008769733.1 , NM_174864.3
Accession Version	XM_008769733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nicastrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGG CTAGGGGCGG CTCTGGGCCT GACCCAGGAA GTCGGGGTCT CCTTCTTCTG 
TCTTTTTCCG TGGTACTAGC AGGATTGTGT GGGGGAAACT CAGTGGAGAG GAAGATCTAC 
ATTCCCTTAA ATAAAACAGC TCCTTGTGTC CGCCTGCTCA ATGCCACTCA TCAGATTGGC 
TGCCAGTCTT CAATTAGTGG GGATACAGGG GTTATCCACG TAGTGGAGAA AGAAGACGAC 
CTGAAATGGG TATTGACGGA TGGCCCCAAC CCCCCTTACA TGGTTCTGCT GGAGGGCAAG 
CTCTTTACCA GAGATATAAT GGAAAAGCTG AAGGGGGAAA CCAGTAGAAT CGCTGGTCTT 
GCCGTGACTC TAGCCAAGCC CAATTCAACG TCAAGCTTCT CTCCTAGCGT GCAGTGCCCA 
AATGATGGGT TTGGTATTTA CTCCAACTCC TATGGGCCAG AGTTTGCTCA CTGCAAAAAA 
ACATTGTGGA ATGAACTGGG CAATGGCTTG GCTTATGACG ACTTCAGTTT CCCCATCTTT 
CTTCTTGAAG ATGAGAACGA AACCAAGGTC ATCAAGCAGT GCTATCAAGA TCACAACCTG 
GGTCAGAATG GCTCTGCACC GAGCTTCCCA CTGTGTGCTA TGCAGCTCTT CTCACACATG 
CACGCCGTCA TCAGCACCGC CACCTGCATG CGACGCAGCT TCATCCAGAG CACCTTCAGC 
ATCAACCCAG AAATCGTCTG TGACCCCTTA TCCGACTACA ACGTATGGAG CATGCTTAAG 
CCTATAAATA CGTCTGGGGG ATTAGAACCT GATGTCAGGG TTGTGGTTGC AGCCACACGG 
CTGGATAGCC GGTCCTTCTT CTGGAATGTG GCCCCAGGGG CAGAAAGTGC CGTTGCCTCC 
TTTGTCACTC AGCTGGCCGC AGCTGAAGCT TTGCACAAGG CACCTGATGT GACCACCCTA 
CCCCGAAATG TGATGTTTGT CTTCTTCCAG GGGGAAACTT TTGACTACAT TGGCAGCTCA 
AGGATGGTCT ATGACATGGA GAACGGCAAG TTTCCTGTGC GGCTAGAGAA CATTGACTCC 
TTTGTGGAGC TGGGGCAGGT GGCCCTAAGG ACGTCACTAG AACTCTGGAT GCACACAGAT 
CCTATGTCTC AGAAAAATGA ATCTGTGAAG AACCAGGTGG AGGATCTTCT GGTCACTCTG 
GAGCAGAGTG GTGCTGATAC CCCTCAAGTT GTCCTGAGCA GACTAGTCCA GTCCCAGGCC 
CTTCCACCAT CATCTCTACA ACGATTTCTT CGGGCTCGAA ACATCTCTGG TGTGGTCTTG 
GCTGACCACT CTGGTTCCTT CCACAATCGG TATTACCAGA GCATTTATGA CACTGCTGAG 
AACATTAACG TGACCTATCC TGAGTCGCAG AGCCCAGAAG AGGACCTCAA CTTTGTAACA 
GACACTGCCA AGGCACTGGC AGATGTTGCC ACAGTGCTGG CACGAGCTTT GTACAAGCTT 
GCAGGAGGAA CCAACTTCAA CAACTCCATT CAGGCTGATC CCCAGACAGT TACACGTCTG 
CTCTATGGGT TCCTGGTCAG AGCTAACAAC TCATGGTTTC AGTCTATCCT CAGACATGAT 
CTGAGGTCCT ATTTGGATGA TGGGCCTCTT CAACACTACA TCGCTGTCTC CAGCCCTACC 
AACACGACTT ACGTTGTGCA GTATGCCTTG GCAAACCTGA CTGGCAAGGT GACCAACCTC 
ACCCAAGAGC AGTGCCAGGA TCCAAGTAAA GTCCCAAATG AAAGCAAGGA TTTGTATGAA 
TACTCGTGGG TACAAGGCCC TTGGAATTCC AACAAGACAG AGCGGCTCCC ACGGTGTGTG 
CGCTCCACAG TACGACTGGC TAGGGCCTTG TCCCCCGCCT TTGAGCTGAG TCAGTGGAGC 
TCCACAGAAT ACTCTACGTG GGCCGAGAGC CGCTGGAAAG ATATCCAAGC TCGGATATTC 
CTCATTGCCA GCAAAGAACT TGAGTTCATC ACGCTGATCG TGGGCTTCAG CATCCTCGTC 
TTTTCTCTCA TCGTCACTTA TTGTATCAAC GCCAAGGCCG ATGTCCTTTT CGTTGCTCCC 
CGAGAGCCAG GAGCTGTGTC TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767955.1
CDS	78..2204
Translation	MATARGGSGPDPGSRGLLLLSFSVVLAGLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEDDLKWVLTDGPNPPYMVLLEGKLFTRDIMEKLKGETSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVTLEQSGADTPQVVLSRLVQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPESQSPEEDLNFVTDTAKALADVATVLARALYKLAGGTNFNNSIQADPQTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAVSSPTNTTYVVQYALANLTGKVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPRCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSILVFSLIVTYCINAKADVLFVAPREPGAVSY

Target ORF information:

RefSeq Version	XM_008769733.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nicastrin (Ncstn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769733.1

ORF Insert Sequence:

ATGGCTACGG CTAGGGGCGG CTCTGGGCCT GACCCAGGAA GTCGGGGTCT CCTTCTTCTG 
TCTTTTTCCG TGGTACTAGC AGGATTGTGT GGGGGAAACT CAGTGGAGAG GAAGATCTAC 
ATTCCCTTAA ATAAAACAGC TCCTTGTGTC CGCCTGCTCA ATGCCACTCA TCAGATTGGC 
TGCCAGTCTT CAATTAGTGG GGATACAGGG GTTATCCACG TAGTGGAGAA AGAAGACGAC 
CTGAAATGGG TATTGACGGA TGGCCCCAAC CCCCCTTACA TGGTTCTGCT GGAGGGCAAG 
CTCTTTACCA GAGATATAAT GGAAAAGCTG AAGGGGGAAA CCAGTAGAAT CGCTGGTCTT 
GCCGTGACTC TAGCCAAGCC CAATTCAACG TCAAGCTTCT CTCCTAGCGT GCAGTGCCCA 
AATGATGGGT TTGGTATTTA CTCCAACTCC TATGGGCCAG AGTTTGCTCA CTGCAAAAAA 
ACATTGTGGA ATGAACTGGG CAATGGCTTG GCTTATGACG ACTTCAGTTT CCCCATCTTT 
CTTCTTGAAG ATGAGAACGA AACCAAGGTC ATCAAGCAGT GCTATCAAGA TCACAACCTG 
GGTCAGAATG GCTCTGCACC GAGCTTCCCA CTGTGTGCTA TGCAGCTCTT CTCACACATG 
CACGCCGTCA TCAGCACCGC CACCTGCATG CGACGCAGCT TCATCCAGAG CACCTTCAGC 
ATCAACCCAG AAATCGTCTG TGACCCCTTA TCCGACTACA ACGTATGGAG CATGCTTAAG 
CCTATAAATA CGTCTGGGGG ATTAGAACCT GATGTCAGGG TTGTGGTTGC AGCCACACGG 
CTGGATAGCC GGTCCTTCTT CTGGAATGTG GCCCCAGGGG CAGAAAGTGC CGTTGCCTCC 
TTTGTCACTC AGCTGGCCGC AGCTGAAGCT TTGCACAAGG CACCTGATGT GACCACCCTA 
CCCCGAAATG TGATGTTTGT CTTCTTCCAG GGGGAAACTT TTGACTACAT TGGCAGCTCA 
AGGATGGTCT ATGACATGGA GAACGGCAAG TTTCCTGTGC GGCTAGAGAA CATTGACTCC 
TTTGTGGAGC TGGGGCAGGT GGCCCTAAGG ACGTCACTAG AACTCTGGAT GCACACAGAT 
CCTATGTCTC AGAAAAATGA ATCTGTGAAG AACCAGGTGG AGGATCTTCT GGTCACTCTG 
GAGCAGAGTG GTGCTGATAC CCCTCAAGTT GTCCTGAGCA GACTAGTCCA GTCCCAGGCC 
CTTCCACCAT CATCTCTACA ACGATTTCTT CGGGCTCGAA ACATCTCTGG TGTGGTCTTG 
GCTGACCACT CTGGTTCCTT CCACAATCGG TATTACCAGA GCATTTATGA CACTGCTGAG 
AACATTAACG TGACCTATCC TGAGTCGCAG AGCCCAGAAG AGGACCTCAA CTTTGTAACA 
GACACTGCCA AGGCACTGGC AGATGTTGCC ACAGTGCTGG CACGAGCTTT GTACAAGCTT 
GCAGGAGGAA CCAACTTCAA CAACTCCATT CAGGCTGATC CCCAGACAGT TACACGTCTG 
CTCTATGGGT TCCTGGTCAG AGCTAACAAC TCATGGTTTC AGTCTATCCT CAGACATGAT 
CTGAGGTCCT ATTTGGATGA TGGGCCTCTT CAACACTACA TCGCTGTCTC CAGCCCTACC 
AACACGACTT ACGTTGTGCA GTATGCCTTG GCAAACCTGA CTGGCAAGGT GACCAACCTC 
ACCCAAGAGC AGTGCCAGGA TCCAAGTAAA GTCCCAAATG AAAGCAAGGA TTTGTATGAA 
TACTCGTGGG TACAAGGCCC TTGGAATTCC AACAAGACAG AGCGGCTCCC ACGGTGTGTG 
CGCTCCACAG TACGACTGGC TAGGGCCTTG TCCCCCGCCT TTGAGCTGAG TCAGTGGAGC 
TCCACAGAAT ACTCTACGTG GGCCGAGAGC CGCTGGAAAG ATATCCAAGC TCGGATATTC 
CTCATTGCCA GCAAAGAACT TGAGTTCATC ACGCTGATCG TGGGCTTCAG CATCCTCGTC 
TTTTCTCTCA TCGTCACTTA TTGTATCAAC GCCAAGGCCG ATGTCCTTTT CGTTGCTCCC 
CGAGAGCCAG GAGCTGTGTC TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vitamin D receptor is present on the neuronal plasma membrane and is co-localized with amyloid precursor protein, ADAM10 or Nicastrin.
PloS one12(11)e0188605(2017)
Dursun E,Gezen-Ak D

Loss of Nicastrin from Oligodendrocytes Results in Hypomyelination and Schizophrenia with Compulsive Behavior.
The Journal of biological chemistry291(22)11647-56(2016 May)
Dries DR,Zhu Y,Brooks MM,Forero DA,Adachi M,Cenik B,West JM,Han YH,Yu C,Arbella J,Nordin A,Adolfsson R,Del-Favero J,Lu QR,Callaerts P,Birnbaum SG,Yu G

An atomic structure of human ?-secretase.
Nature525(7568)212-217(2015 Sep)
Bai XC,Yan C,Yang G,Lu P,Ma D,Sun L,Zhou R,Scheres SHW,Shi Y

Pre-synaptic localization of the ?-secretase-inhibiting protein p24?2 in the mammalian brain.
Journal of neurochemistry133(3)422-31(2015 May)
Liu L,Fujino K,Nishimura M

An aberrant sugar modification of BACE1 blocks its lysosomal targeting in Alzheimer's disease.
EMBO molecular medicine7(2)175-89(2015 Feb)
Kizuka Y,Kitazume S,Fujinawa R,Saito T,Iwata N,Saido TC,Nakano M,Yamaguchi Y,Hashimoto Y,Staufenbiel M,Hatsuta H,Murayama S,Manya H,Endo T,Taniguchi N

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Ncstn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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