ORF » Species Summary » Rattus norvegicus » Nit1 cDNA ORF clone

Nit1 cDNA ORF clone, Rattus norvegicus(Norway rat)

  • Gene

  • Clones

  • gRNAs

The following Nit1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nit1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa04522 NM_182668.2
Latest version!		 Rattus norvegicus nitrilase 1 (Nit1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
ORa10882 NM_001305647.1
Latest version!		 Rattus norvegicus nitrilase 1 (Nit1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
ORa10882 NM_001082580.1
Latest version!		 Rattus norvegicus nitrilase 1 (Nit1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
ORa44767 NM_001305646.1
Latest version!		 Rattus norvegicus nitrilase 1 (Nit1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    Nit1 ( NM_182668.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_872609.2 Rattus norvegicus nitrilase 1 (Nit1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID ORa04522
    Clone ID Related Accession (Same CDS sequence) NM_182668.2
    Accession Version NM_182668.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Rattus norvegicus(Norway rat)
    Product deaminated glutathione amidase isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC099236.8 and BC087146.1. On Mar 10, 2007 this sequence version replaced NM_182668.1. Transcript Variant: This variant (1) encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC087146.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGGGCT TCATCACCAG GCCTCCTCAT CAACTCCTGT CCCTTCTGTG TACCGGATAC 
    CGATTACCTC AAATCTCAGT ACTTTGTACT CAGCCCAGGC CCAGAGCCAT GGCAGAGTCC 
    TCATCGACTT CCTGGGAGCT GCCCCTGGTG GCTGTGTGCC AGGTAACATC AACACCAAAC 
    AAGCAAGAGA ACTTTAAAAC GTGTGCTGAG CTGGTTCAAG AGGCTACCAG ACTGGGCGCT 
    TGCCTGGCCT TTCTGCCTGA GGCATTTGAC TTTATTGCAC GAAATCCTGC CGAGACATTA 
    CTCCTGTCCG AACCACTGGA TGGGGACCTT TTGGGACAAT ATAGCCAGCT TGCCAGGGAA 
    TGTGGAATCT GGCTGTCCTT GGGTGGTTTC CACGAGCGTG GCCAAGACTG GGAGCAGACT 
    CAGAAAATCT ACAATTGTCA TGTGCTTCTG AACAGCAAGG GATCAGTAGT GGCCAGTTAC 
    AGGAAGACAC ATCTATGTGA TGTAGAGATC CCAGGTCAGG GGCCTATGAG AGAAAGCAAC 
    TATACCATGC CTGGATACGC TCTTGAACCA CCTGTCAAGA CACCAGCAGG CAAGGTTGGT 
    CTAGCAATCT GTTATGACAT GCGGTTCCCT GAACTTTCTC TGAAATTGGC TCAAGCTGGG 
    GCAGAAATAC TTACTTATCC TTCAGCCTTT GGATCTGTTA CCGGCCCAGC CCACTGGGAG 
    GTGCTGCTGC GGGCCCGCGC CATTGAGTCT CAGTGCTACG TGATAGCAGC AGCACAGTGT 
    GGACGCCACC ATGAGACAAG AGCAAGTTAC GGCCACAGCA TGGTGGTTGA CCCCTGGGGC 
    ACAGTGGTGG CCAGCTGTTC CGAGGGACCA GGCCTTTGCC TTGCTCGAAT TGATCTCCAC 
    TTTCTGCAAC AGATGCGCCA ACACTTGCCT GTGTTCCAGC ATCGCAGACC TGACCTTTAT 
    GGCAGTCTCC TGCCACTCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_872609.2
    CDS76..1059
    Translation

    Target ORF information:

    RefSeq Version NM_182668.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus nitrilase 1 (Nit1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_182668.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCTGGGCT TCATCACCAG GCCTCCTCAT CAACTCCTGT CCCTTCTGTG TACCGGATAC 
    CGATTACCTC AAATCTCAGT ACTTTGTACT CAGCCCAGGC CCAGAGCCAT GGCAGAGTCC 
    TCATCGACTT CCTGGGAGCT GCCCCTGGTG GCTGTGTGCC AGGTAACATC AACACCAAAC 
    AAGCAAGAGA ACTTTAAAAC GTGTGCTGAG CTGGTTCAAG AGGCTACCAG ACTGGGCGCT 
    TGCCTGGCCT TTCTGCCTGA GGCATTTGAC TTTATTGCAC GAAATCCTGC CGAGACATTA 
    CTCCTGTCCG AACCACTGGA TGGGGACCTT TTGGGACAAT ATAGCCAGCT TGCCAGGGAA 
    TGTGGAATCT GGCTGTCCTT GGGTGGTTTC CACGAGCGTG GCCAAGACTG GGAGCAGACT 
    CAGAAAATCT ACAATTGTCA TGTGCTTCTG AACAGCAAGG GATCAGTAGT GGCCAGTTAC 
    AGGAAGACAC ATCTATGTGA TGTAGAGATC CCAGGTCAGG GGCCTATGAG AGAAAGCAAC 
    TATACCATGC CTGGATACGC TCTTGAACCA CCTGTCAAGA CACCAGCAGG CAAGGTTGGT 
    CTAGCAATCT GTTATGACAT GCGGTTCCCT GAACTTTCTC TGAAATTGGC TCAAGCTGGG 
    GCAGAAATAC TTACTTATCC TTCAGCCTTT GGATCTGTTA CCGGCCCAGC CCACTGGGAG 
    GTGCTGCTGC GGGCCCGCGC CATTGAGTCT CAGTGCTACG TGATAGCAGC AGCACAGTGT 
    GGACGCCACC ATGAGACAAG AGCAAGTTAC GGCCACAGCA TGGTGGTTGA CCCCTGGGGC 
    ACAGTGGTGG CCAGCTGTTC CGAGGGACCA GGCCTTTGCC TTGCTCGAAT TGATCTCCAC 
    TTTCTGCAAC AGATGCGCCA ACACTTGCCT GTGTTCCAGC ATCGCAGACC TGACCTTTAT 
    GGCAGTCTCC TGCCACTCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nit1 ( NM_001305647.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001292576.1 Rattus norvegicus nitrilase 1 (Nit1), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID ORa10882
    Clone ID Related Accession (Same CDS sequence) NM_001305647.1 , NM_001082580.1
    Accession Version NM_001305647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Rattus norvegicus(Norway rat)
    Product deaminated glutathione amidase isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CV073582.1 and BC087146.1. Transcript Variant: This variant (4) differs in the 5' UTR and uses a downstream start codon compared to variant 1. It encodes isoform b, which has a shorter N-terminus than isoform a. Variants 2 and 4 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: CV073582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00632251, SAMN00632256 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCAGAGT CCTCATCGAC TTCCTGGGAG CTGCCCCTGG TGGCTGTGTG CCAGGTAACA 
    TCAACACCAA ACAAGCAAGA GAACTTTAAA ACGTGTGCTG AGCTGGTTCA AGAGGCTACC 
    AGACTGGGCG CTTGCCTGGC CTTTCTGCCT GAGGCATTTG ACTTTATTGC ACGAAATCCT 
    GCCGAGACAT TACTCCTGTC CGAACCACTG GATGGGGACC TTTTGGGACA ATATAGCCAG 
    CTTGCCAGGG AATGTGGAAT CTGGCTGTCC TTGGGTGGTT TCCACGAGCG TGGCCAAGAC 
    TGGGAGCAGA CTCAGAAAAT CTACAATTGT CATGTGCTTC TGAACAGCAA GGGATCAGTA 
    GTGGCCAGTT ACAGGAAGAC ACATCTATGT GATGTAGAGA TCCCAGGTCA GGGGCCTATG 
    AGAGAAAGCA ACTATACCAT GCCTGGATAC GCTCTTGAAC CACCTGTCAA GACACCAGCA 
    GGCAAGGTTG GTCTAGCAAT CTGTTATGAC ATGCGGTTCC CTGAACTTTC TCTGAAATTG 
    GCTCAAGCTG GGGCAGAAAT ACTTACTTAT CCTTCAGCCT TTGGATCTGT TACCGGCCCA 
    GCCCACTGGG AGGTGCTGCT GCGGGCCCGC GCCATTGAGT CTCAGTGCTA CGTGATAGCA 
    GCAGCACAGT GTGGACGCCA CCATGAGACA AGAGCAAGTT ACGGCCACAG CATGGTGGTT 
    GACCCCTGGG GCACAGTGGT GGCCAGCTGT TCCGAGGGAC CAGGCCTTTG CCTTGCTCGA 
    ATTGATCTCC ACTTTCTGCA ACAGATGCGC CAACACTTGC CTGTGTTCCA GCATCGCAGA 
    CCTGACCTTT ATGGCAGTCT CCTGCCACTC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292576.1
    CDS76..951
    Translation

    Target ORF information:

    RefSeq Version NM_001305647.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus nitrilase 1 (Nit1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001305647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCAGAGT CCTCATCGAC TTCCTGGGAG CTGCCCCTGG TGGCTGTGTG CCAGGTAACA 
    TCAACACCAA ACAAGCAAGA GAACTTTAAA ACGTGTGCTG AGCTGGTTCA AGAGGCTACC 
    AGACTGGGCG CTTGCCTGGC CTTTCTGCCT GAGGCATTTG ACTTTATTGC ACGAAATCCT 
    GCCGAGACAT TACTCCTGTC CGAACCACTG GATGGGGACC TTTTGGGACA ATATAGCCAG 
    CTTGCCAGGG AATGTGGAAT CTGGCTGTCC TTGGGTGGTT TCCACGAGCG TGGCCAAGAC 
    TGGGAGCAGA CTCAGAAAAT CTACAATTGT CATGTGCTTC TGAACAGCAA GGGATCAGTA 
    GTGGCCAGTT ACAGGAAGAC ACATCTATGT GATGTAGAGA TCCCAGGTCA GGGGCCTATG 
    AGAGAAAGCA ACTATACCAT GCCTGGATAC GCTCTTGAAC CACCTGTCAA GACACCAGCA 
    GGCAAGGTTG GTCTAGCAAT CTGTTATGAC ATGCGGTTCC CTGAACTTTC TCTGAAATTG 
    GCTCAAGCTG GGGCAGAAAT ACTTACTTAT CCTTCAGCCT TTGGATCTGT TACCGGCCCA 
    GCCCACTGGG AGGTGCTGCT GCGGGCCCGC GCCATTGAGT CTCAGTGCTA CGTGATAGCA 
    GCAGCACAGT GTGGACGCCA CCATGAGACA AGAGCAAGTT ACGGCCACAG CATGGTGGTT 
    GACCCCTGGG GCACAGTGGT GGCCAGCTGT TCCGAGGGAC CAGGCCTTTG CCTTGCTCGA 
    ATTGATCTCC ACTTTCTGCA ACAGATGCGC CAACACTTGC CTGTGTTCCA GCATCGCAGA 
    CCTGACCTTT ATGGCAGTCT CCTGCCACTC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nit1 ( NM_001082580.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001076049.1 Rattus norvegicus nitrilase 1 (Nit1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID ORa10882
    Clone ID Related Accession (Same CDS sequence) NM_001305647.1 , NM_001082580.1
    Accession Version NM_001082580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Rattus norvegicus(Norway rat)
    Product deaminated glutathione amidase isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC099236.8, BC087146.1 and FM045849.1. Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the 5' coding region, which results in initiation of translation from a downstream start codon compared to variant 1. It encodes isoform b, which has a shorter N-terminus compared to isoform a. Variants 2 and 4 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: FQ214471.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCAGAGT CCTCATCGAC TTCCTGGGAG CTGCCCCTGG TGGCTGTGTG CCAGGTAACA 
    TCAACACCAA ACAAGCAAGA GAACTTTAAA ACGTGTGCTG AGCTGGTTCA AGAGGCTACC 
    AGACTGGGCG CTTGCCTGGC CTTTCTGCCT GAGGCATTTG ACTTTATTGC ACGAAATCCT 
    GCCGAGACAT TACTCCTGTC CGAACCACTG GATGGGGACC TTTTGGGACA ATATAGCCAG 
    CTTGCCAGGG AATGTGGAAT CTGGCTGTCC TTGGGTGGTT TCCACGAGCG TGGCCAAGAC 
    TGGGAGCAGA CTCAGAAAAT CTACAATTGT CATGTGCTTC TGAACAGCAA GGGATCAGTA 
    GTGGCCAGTT ACAGGAAGAC ACATCTATGT GATGTAGAGA TCCCAGGTCA GGGGCCTATG 
    AGAGAAAGCA ACTATACCAT GCCTGGATAC GCTCTTGAAC CACCTGTCAA GACACCAGCA 
    GGCAAGGTTG GTCTAGCAAT CTGTTATGAC ATGCGGTTCC CTGAACTTTC TCTGAAATTG 
    GCTCAAGCTG GGGCAGAAAT ACTTACTTAT CCTTCAGCCT TTGGATCTGT TACCGGCCCA 
    GCCCACTGGG AGGTGCTGCT GCGGGCCCGC GCCATTGAGT CTCAGTGCTA CGTGATAGCA 
    GCAGCACAGT GTGGACGCCA CCATGAGACA AGAGCAAGTT ACGGCCACAG CATGGTGGTT 
    GACCCCTGGG GCACAGTGGT GGCCAGCTGT TCCGAGGGAC CAGGCCTTTG CCTTGCTCGA 
    ATTGATCTCC ACTTTCTGCA ACAGATGCGC CAACACTTGC CTGTGTTCCA GCATCGCAGA 
    CCTGACCTTT ATGGCAGTCT CCTGCCACTC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001076049.1
    CDS216..1091
    Translation

    Target ORF information:

    RefSeq Version NM_001082580.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus nitrilase 1 (Nit1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001082580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGCAGAGT CCTCATCGAC TTCCTGGGAG CTGCCCCTGG TGGCTGTGTG CCAGGTAACA 
    TCAACACCAA ACAAGCAAGA GAACTTTAAA ACGTGTGCTG AGCTGGTTCA AGAGGCTACC 
    AGACTGGGCG CTTGCCTGGC CTTTCTGCCT GAGGCATTTG ACTTTATTGC ACGAAATCCT 
    GCCGAGACAT TACTCCTGTC CGAACCACTG GATGGGGACC TTTTGGGACA ATATAGCCAG 
    CTTGCCAGGG AATGTGGAAT CTGGCTGTCC TTGGGTGGTT TCCACGAGCG TGGCCAAGAC 
    TGGGAGCAGA CTCAGAAAAT CTACAATTGT CATGTGCTTC TGAACAGCAA GGGATCAGTA 
    GTGGCCAGTT ACAGGAAGAC ACATCTATGT GATGTAGAGA TCCCAGGTCA GGGGCCTATG 
    AGAGAAAGCA ACTATACCAT GCCTGGATAC GCTCTTGAAC CACCTGTCAA GACACCAGCA 
    GGCAAGGTTG GTCTAGCAAT CTGTTATGAC ATGCGGTTCC CTGAACTTTC TCTGAAATTG 
    GCTCAAGCTG GGGCAGAAAT ACTTACTTAT CCTTCAGCCT TTGGATCTGT TACCGGCCCA 
    GCCCACTGGG AGGTGCTGCT GCGGGCCCGC GCCATTGAGT CTCAGTGCTA CGTGATAGCA 
    GCAGCACAGT GTGGACGCCA CCATGAGACA AGAGCAAGTT ACGGCCACAG CATGGTGGTT 
    GACCCCTGGG GCACAGTGGT GGCCAGCTGT TCCGAGGGAC CAGGCCTTTG CCTTGCTCGA 
    ATTGATCTCC ACTTTCTGCA ACAGATGCGC CAACACTTGC CTGTGTTCCA GCATCGCAGA 
    CCTGACCTTT ATGGCAGTCT CCTGCCACTC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    Nit1 ( NM_001305646.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001292575.1 Rattus norvegicus nitrilase 1 (Nit1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID ORa44767
    Clone ID Related Accession (Same CDS sequence) NM_001305646.1
    Accession Version NM_001305646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Rattus norvegicus(Norway rat)
    Product deaminated glutathione amidase isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC099236.8 and BC087146.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform c, which is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: CO563648.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCCTCCTC ATCAACTCCT GTCCCTTCTG TGTACCGGAT ACCGATTACC TCAAATCTCA 
    GTACTTTGTA CTCAGCCCAG GCCCAGAGCC ATGGCAGAGT CCTCATCGAC TTCCTGGGAG 
    CTGCCCCTGG TGGCTGTGTG CCAGGTAACA TCAACACCAA ACAAGCAAGA GAACTTTAAA 
    ACGTGTGCTG AGCTGGTTCA AGAGGCTACC AGACTGGGCG CTTGCCTGGC CTTTCTGCCT 
    GAGGCATTTG ACTTTATTGC ACGAAATCCT GCCGAGACAT TACTCCTGTC CGAACCACTG 
    GATGGGGACC TTTTGGGACA ATATAGCCAG CTTGCCAGGG AATGTGGAAT CTGGCTGTCC 
    TTGGGTGGTT TCCACGAGCG TGGCCAAGAC TGGGAGCAGA CTCAGAAAAT CTACAATTGT 
    CATGTGCTTC TGAACAGCAA GGGATCAGTA GTGGCCAGTT ACAGGAAGAC ACATCTATGT 
    GATGTAGAGA TCCCAGGTCA GGGGCCTATG AGAGAAAGCA ACTATACCAT GCCTGGATAC 
    GCTCTTGAAC CACCTGTCAA GACACCAGCA GGCAAGGTTG GTCTAGCAAT CTGTTATGAC 
    ATGCGGTTCC CTGAACTTTC TCTGAAATTG GCTCAAGCTG GGGCAGAAAT ACTTACTTAT 
    CCTTCAGCCT TTGGATCTGT TACCGGCCCA GCCCACTGGG AGGTGCTGCT GCGGGCCCGC 
    GCCATTGAGT CTCAGTGCTA CGTGATAGCA GCAGCACAGT GTGGACGCCA CCATGAGACA 
    AGAGCAAGTT ACGGCCACAG CATGGTGGTT GACCCCTGGG GCACAGTGGT GGCCAGCTGT 
    TCCGAGGGAC CAGGCCTTTG CCTTGCTCGA ATTGATCTCC ACTTTCTGCA ACAGATGCGC 
    CAACACTTGC CTGTGTTCCA GCATCGCAGA CCTGACCTTT ATGGCAGTCT CCTGCCACTC 
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001292575.1
    CDS76..1041
    Translation

    Target ORF information:

    RefSeq Version NM_001305646.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus nitrilase 1 (Nit1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001305646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCCTCCTC ATCAACTCCT GTCCCTTCTG TGTACCGGAT ACCGATTACC TCAAATCTCA 
    GTACTTTGTA CTCAGCCCAG GCCCAGAGCC ATGGCAGAGT CCTCATCGAC TTCCTGGGAG 
    CTGCCCCTGG TGGCTGTGTG CCAGGTAACA TCAACACCAA ACAAGCAAGA GAACTTTAAA 
    ACGTGTGCTG AGCTGGTTCA AGAGGCTACC AGACTGGGCG CTTGCCTGGC CTTTCTGCCT 
    GAGGCATTTG ACTTTATTGC ACGAAATCCT GCCGAGACAT TACTCCTGTC CGAACCACTG 
    GATGGGGACC TTTTGGGACA ATATAGCCAG CTTGCCAGGG AATGTGGAAT CTGGCTGTCC 
    TTGGGTGGTT TCCACGAGCG TGGCCAAGAC TGGGAGCAGA CTCAGAAAAT CTACAATTGT 
    CATGTGCTTC TGAACAGCAA GGGATCAGTA GTGGCCAGTT ACAGGAAGAC ACATCTATGT 
    GATGTAGAGA TCCCAGGTCA GGGGCCTATG AGAGAAAGCA ACTATACCAT GCCTGGATAC 
    GCTCTTGAAC CACCTGTCAA GACACCAGCA GGCAAGGTTG GTCTAGCAAT CTGTTATGAC 
    ATGCGGTTCC CTGAACTTTC TCTGAAATTG GCTCAAGCTG GGGCAGAAAT ACTTACTTAT 
    CCTTCAGCCT TTGGATCTGT TACCGGCCCA GCCCACTGGG AGGTGCTGCT GCGGGCCCGC 
    GCCATTGAGT CTCAGTGCTA CGTGATAGCA GCAGCACAGT GTGGACGCCA CCATGAGACA 
    AGAGCAAGTT ACGGCCACAG CATGGTGGTT GACCCCTGGG GCACAGTGGT GGCCAGCTGT 
    TCCGAGGGAC CAGGCCTTTG CCTTGCTCGA ATTGATCTCC ACTTTCTGCA ACAGATGCGC 
    CAACACTTGC CTGTGTTCCA GCATCGCAGA CCTGACCTTT ATGGCAGTCT CCTGCCACTC 
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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