Ddx5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx5
Entrez Gene ID	287765
Full Name	DEAD-box helicase 5
General protein information	Preferred Names DEAD-box helicase 5 Names probable ATP-dependent RNA helicase DDX5 DEAD (Asp-Glu-Ala-Asp) box helicase 5 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 ddx5 gene protein p68 RNA helicase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	mouse homolog is a RNA helicase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007613.1	NP_001007614.1	probable ATP-dependent RNA helicase DDX5
XM_008768313.2	XP_008766535.1	probable ATP-dependent RNA helicase DDX5 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DDX5	XP_005624321.1
Gallus gallus (chicken)	DDX5	NP_990158.1
Kluyveromyces lactis	KLLA0F23716g	XP_456137.1
Schizosaccharomyces pombe (fission yeast)	dbp2	NP_596523.1
Rattus norvegicus (Norway rat)	Ddx5	NP_001007614.1
Saccharomyces cerevisiae (baker's yeast)	DBP2	NP_014287.3
Eremothecium gossypii	AGOS_AFL221C	NP_985329.2
Arabidopsis thaliana (thale cress)	AT5G63120	NP_974985.1
Homo sapiens (human)	DDX5	NP_004387.1
Bos taurus (cattle)	DDX5	NP_001178324.1
Mus musculus (house mouse)	Ddx5	NP_031866.2
Drosophila melanogaster (fruit fly)	CG10077	NP_648062.2
Neurospora crassa	NCU07839	XP_962960.2
Arabidopsis thaliana (thale cress)	AT1G55150	NP_175911.1
Pan troglodytes (chimpanzee)	DDX5	NP_001138306.1
Magnaporthe oryzae (rice blast fungus)	MGG_16901	XP_003712846.1
Xenopus tropicalis (tropical clawed frog)	ddx5	NP_989229.1
	DDX5	XP_001109860.1
Danio rerio (zebrafish)	ddx5	NP_997777.1

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of RNA helicase p68 in skin wound healing process in rats.
Wang SJ, Du L, Shi CM
Chinese journal of traumatology = Zhonghua chuang shang za zhi20(6)311-317(2017 Dec)

Macrophage migration inhibitory factor triggers vascular smooth muscle cell dedifferentiation by a p68-serum response factor axis.
Fan Y, Zhang J, Chen CY, Xiao YB, Asico LD, Jose PA, Xu JC, Qian GS, Zeng CY
Cardiovascular research113(5)519-530(2017 Apr)

JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.
Berg JM, Lee C, Chen L, Galvan L, Cepeda C, Chen JY, Pe?agarikano O, Stein JL, Li A, Oguro-Ando A, Miller JA, Vashisht AA, Starks ME, Kite EP, Tam E, Gdalyahu A, Al-Sharif NB, Burkett ZD, White SA, Fears SC, Levine MS, Wohlschlegel JA, Geschwind DH
Neuron88(6)1173-91(2015 Dec)

RNA helicases DDX5 and DDX17 dynamically orchestrate transcription, miRNA, and splicing programs in cell differentiation.
Dardenne E, Polay Espinoza M, Fattet L, Germann S, Lambert MP, Neil H, Zonta E, Mortada H, Gratadou L, Deygas M, Chakrama FZ, Samaan S, Desmet FO, Tranchevent LC, Dutertre M, Rimokh R, Bourgeois CF, Auboeuf D
Cell reports7(6)1900-13(2014 Jun)

The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways.
Samaan S, Tranchevent LC, Dardenne E, Polay Espinoza M, Zonta E, Germann S, Gratadou L, Dutertre M, Auboeuf D
Nucleic acids research42(4)2197-207(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MIF triggers vascular smooth muscle cell dedifferentiation by a p68-serum response factor signaling axis.
Title: Macrophage migration inhibitory factor triggers vascular smooth muscle cell dedifferentiation by a p68-serum response factor axis.

The following Ddx5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11133	NM_001007613.1 Latest version!	Rattus norvegicus DEAD-box helicase 5 (Ddx5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa11133	XM_008768313.2 Latest version!	Rattus norvegicus DEAD-box helicase 5 (Ddx5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx5 ( NM_001007613.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007614.1 Rattus norvegicus DEAD-box helicase 5 (Ddx5), mRNA.

Price & Availability↑

CloneID	ORa11133
Clone ID Related Accession (Same CDS sequence)	NM_001007613.1 , XM_008768313.2
Accession Version	NM_001007613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DDX5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079036.1. On Nov 29, 2004 this sequence version replaced XM_213508.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC079036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906351 [ECO:0000348] ##Evidence-Data-END##

ATGTCGAGTT ATTCTAGTGA CCGAGACCGC GGCCGGGATC GAGGGTTTGG TGCACCTCGA 
TTTGGAGGGA GTAGAACAGG ACCCCTATCT GGAAAGAAGT TTGGAAATCC TGGGGAGAAA 
CTAGTTAAAA AGAAGTGGAA TCTTGATGAG CTGCCCAAAT TTGAGAAGAA TTTTTATCAG 
GAACACCCTG ATTTGGCAAG GCGCACCGCA CAAGAGGTAG ATACGTACAG AAGAAGCAAG 
GAAATTACAG TCAGAGGTCA CAACTGTCCA AAACCTGTTC TGAATTTTTA TGAAGCAAAC 
TTTCCTGCAA ATGTCATGGA TGTGATTGCA AGGCAGAACT TCACTGAACC CACTGCTATC 
CAAGCTCAGG GCTGGCCAGT TGCTCTCAGT GGATTGGATA TGGTTGGAGT AGCTCAGACT 
GGATCTGGGA AAACATTATC TTATTTGCTG CCTGCCATTG TACACATAAA CCACCAGCCA 
TTCTTAGAGA GAGGTGATGG GCCTATTTGC TTGGTGCTGG CACCAACTCG AGAACTGGCA 
CAGCAGGTGC AGCAGGTCGC TGCTGAATAT TGTAGAGCTT GTCGCTTGAA GTCTACTTGC 
ATCTATGGTG GTGCTCCCAA AGGACCGCAG ATTCGTGATT TGGAGAGAGG TGTGGAAATC 
TGTATTGCAA CACCTGGAAG ACTGATTGAC TTTTTAGAGT GTGGGAAAAC CAATCTGAGA 
AGAACAACTT ACCTTGTCCT TGATGAAGCT GATAGGATGC TTGATATGGG CTTTGAACCC 
CAAATAAGGA AAATTGTGGA TCAAATAAGA CCTGATAGGC AAACACTAAT GTGGAGTGCA 
ACTTGGCCAA AAGAAGTAAG ACAGCTTGCT GAAGATTTCC TGAAAGACTA TATTCATATC 
AATATTGGTG CACTGGAACT GAGTGCAAAC CATAACATTC TTCAGATTGT GGATGTGTGT 
CATGATGTCG AAAAGGATGA AAAACTTATT CGTCTGATGG AAGAGATCAT GAGTGAGAAG 
GAGAATAAAA CCATTGTTTT TGTTGAAACC AAAAGAAGAT GTGATGAACT TACCAGAAAA 
ATGAGGAGAG ATGGGTGGCC TGCCATGGGC ATCCATGGTG ACAAGAGTCA ACAGGAACGA 
GACTGGGTTC TAAATGAATT CAAACATGGA AAAGCTCCTA TTCTGATTGC TACCGATGTG 
GCCTCCAGAG GGCTAGATGT GGAAGATGTG AAATTTGTCA TCAATTATGA CTACCCTAAC 
TCCTCAGAGG ATTATATTCA TCGAATTGGA AGAACTGCTC GCAGTACCAA AACAGGCACA 
GCATACACTT TCTTTACTCC CAATAACATA AAGCAAGTGA GCGACCTCAT CTCTGTGCTT 
CGGGAAGCCA ATCAAGCAAT TAATCCCAAG TTGCTTCAGT TGGTCGAAGA CAGAGGTTCA 
GGTCGTTCCA GGGGTAGAGG AGGCATGAAG GATGATCGTC GTGACAGATA CTCTGCAGGG 
AAAAGGGGTG GATTTAATAC CTTTAGAGAC AGGGAAAACT ATGACAGAGG CTACTCTAGT 
CTGCTTAAGA GAGATTTTGG GGCTAAAACT CAGAATGGTG TTTACAGTGC TGCAAATTAC 
ACCAATGGGA GCTTTGGAAG TAATTTTGTA TCTGCTGGCA TACAGACCAG TTTTAGGACT 
GGTAACCCAA CAGGGACTTA CCAGAACGGT TATGATAGCA CTCAGCAATA TGGAAGTAAT 
GTTGCAAATA TGCACAATGG GATGAACCAA CAGGCATATG CATATCCTGC TACCGCAGCT 
GCTGCGCCTA TGATTGGCTA TCCAATGCCA ACAGGGTATT CTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007614.1
CDS	231..2078
Translation	MSSYSSDRDRGRDRGFGAPRFGGSRTGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVDTYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVANMHNGMNQQAYAYPATAAAAPMIGYPMPTGYSQ

Target ORF information:

RefSeq Version	NM_001007613.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 5 (Ddx5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007613.1

ORF Insert Sequence:

ATGTCGAGTT ATTCTAGTGA CCGAGACCGC GGCCGGGATC GAGGGTTTGG TGCACCTCGA 
TTTGGAGGGA GTAGAACAGG ACCCCTATCT GGAAAGAAGT TTGGAAATCC TGGGGAGAAA 
CTAGTTAAAA AGAAGTGGAA TCTTGATGAG CTGCCCAAAT TTGAGAAGAA TTTTTATCAG 
GAACACCCTG ATTTGGCAAG GCGCACCGCA CAAGAGGTAG ATACGTACAG AAGAAGCAAG 
GAAATTACAG TCAGAGGTCA CAACTGTCCA AAACCTGTTC TGAATTTTTA TGAAGCAAAC 
TTTCCTGCAA ATGTCATGGA TGTGATTGCA AGGCAGAACT TCACTGAACC CACTGCTATC 
CAAGCTCAGG GCTGGCCAGT TGCTCTCAGT GGATTGGATA TGGTTGGAGT AGCTCAGACT 
GGATCTGGGA AAACATTATC TTATTTGCTG CCTGCCATTG TACACATAAA CCACCAGCCA 
TTCTTAGAGA GAGGTGATGG GCCTATTTGC TTGGTGCTGG CACCAACTCG AGAACTGGCA 
CAGCAGGTGC AGCAGGTCGC TGCTGAATAT TGTAGAGCTT GTCGCTTGAA GTCTACTTGC 
ATCTATGGTG GTGCTCCCAA AGGACCGCAG ATTCGTGATT TGGAGAGAGG TGTGGAAATC 
TGTATTGCAA CACCTGGAAG ACTGATTGAC TTTTTAGAGT GTGGGAAAAC CAATCTGAGA 
AGAACAACTT ACCTTGTCCT TGATGAAGCT GATAGGATGC TTGATATGGG CTTTGAACCC 
CAAATAAGGA AAATTGTGGA TCAAATAAGA CCTGATAGGC AAACACTAAT GTGGAGTGCA 
ACTTGGCCAA AAGAAGTAAG ACAGCTTGCT GAAGATTTCC TGAAAGACTA TATTCATATC 
AATATTGGTG CACTGGAACT GAGTGCAAAC CATAACATTC TTCAGATTGT GGATGTGTGT 
CATGATGTCG AAAAGGATGA AAAACTTATT CGTCTGATGG AAGAGATCAT GAGTGAGAAG 
GAGAATAAAA CCATTGTTTT TGTTGAAACC AAAAGAAGAT GTGATGAACT TACCAGAAAA 
ATGAGGAGAG ATGGGTGGCC TGCCATGGGC ATCCATGGTG ACAAGAGTCA ACAGGAACGA 
GACTGGGTTC TAAATGAATT CAAACATGGA AAAGCTCCTA TTCTGATTGC TACCGATGTG 
GCCTCCAGAG GGCTAGATGT GGAAGATGTG AAATTTGTCA TCAATTATGA CTACCCTAAC 
TCCTCAGAGG ATTATATTCA TCGAATTGGA AGAACTGCTC GCAGTACCAA AACAGGCACA 
GCATACACTT TCTTTACTCC CAATAACATA AAGCAAGTGA GCGACCTCAT CTCTGTGCTT 
CGGGAAGCCA ATCAAGCAAT TAATCCCAAG TTGCTTCAGT TGGTCGAAGA CAGAGGTTCA 
GGTCGTTCCA GGGGTAGAGG AGGCATGAAG GATGATCGTC GTGACAGATA CTCTGCAGGG 
AAAAGGGGTG GATTTAATAC CTTTAGAGAC AGGGAAAACT ATGACAGAGG CTACTCTAGT 
CTGCTTAAGA GAGATTTTGG GGCTAAAACT CAGAATGGTG TTTACAGTGC TGCAAATTAC 
ACCAATGGGA GCTTTGGAAG TAATTTTGTA TCTGCTGGCA TACAGACCAG TTTTAGGACT 
GGTAACCCAA CAGGGACTTA CCAGAACGGT TATGATAGCA CTCAGCAATA TGGAAGTAAT 
GTTGCAAATA TGCACAATGG GATGAACCAA CAGGCATATG CATATCCTGC TACCGCAGCT 
GCTGCGCCTA TGATTGGCTA TCCAATGCCA ACAGGGTATT CTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx5 ( XM_008768313.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766535.1 Rattus norvegicus DEAD-box helicase 5 (Ddx5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11133
Clone ID Related Accession (Same CDS sequence)	NM_001007613.1 , XM_008768313.2
Accession Version	XM_008768313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DDX5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAGTT ATTCTAGTGA CCGAGACCGC GGCCGGGATC GAGGGTTTGG TGCACCTCGA 
TTTGGAGGGA GTAGAACAGG ACCCCTATCT GGAAAGAAGT TTGGAAATCC TGGGGAGAAA 
CTAGTTAAAA AGAAGTGGAA TCTTGATGAG CTGCCCAAAT TTGAGAAGAA TTTTTATCAG 
GAACACCCTG ATTTGGCAAG GCGCACCGCA CAAGAGGTAG ATACGTACAG AAGAAGCAAG 
GAAATTACAG TCAGAGGTCA CAACTGTCCA AAACCTGTTC TGAATTTTTA TGAAGCAAAC 
TTTCCTGCAA ATGTCATGGA TGTGATTGCA AGGCAGAACT TCACTGAACC CACTGCTATC 
CAAGCTCAGG GCTGGCCAGT TGCTCTCAGT GGATTGGATA TGGTTGGAGT AGCTCAGACT 
GGATCTGGGA AAACATTATC TTATTTGCTG CCTGCCATTG TACACATAAA CCACCAGCCA 
TTCTTAGAGA GAGGTGATGG GCCTATTTGC TTGGTGCTGG CACCAACTCG AGAACTGGCA 
CAGCAGGTGC AGCAGGTCGC TGCTGAATAT TGTAGAGCTT GTCGCTTGAA GTCTACTTGC 
ATCTATGGTG GTGCTCCCAA AGGACCGCAG ATTCGTGATT TGGAGAGAGG TGTGGAAATC 
TGTATTGCAA CACCTGGAAG ACTGATTGAC TTTTTAGAGT GTGGGAAAAC CAATCTGAGA 
AGAACAACTT ACCTTGTCCT TGATGAAGCT GATAGGATGC TTGATATGGG CTTTGAACCC 
CAAATAAGGA AAATTGTGGA TCAAATAAGA CCTGATAGGC AAACACTAAT GTGGAGTGCA 
ACTTGGCCAA AAGAAGTAAG ACAGCTTGCT GAAGATTTCC TGAAAGACTA TATTCATATC 
AATATTGGTG CACTGGAACT GAGTGCAAAC CATAACATTC TTCAGATTGT GGATGTGTGT 
CATGATGTCG AAAAGGATGA AAAACTTATT CGTCTGATGG AAGAGATCAT GAGTGAGAAG 
GAGAATAAAA CCATTGTTTT TGTTGAAACC AAAAGAAGAT GTGATGAACT TACCAGAAAA 
ATGAGGAGAG ATGGGTGGCC TGCCATGGGC ATCCATGGTG ACAAGAGTCA ACAGGAACGA 
GACTGGGTTC TAAATGAATT CAAACATGGA AAAGCTCCTA TTCTGATTGC TACCGATGTG 
GCCTCCAGAG GGCTAGATGT GGAAGATGTG AAATTTGTCA TCAATTATGA CTACCCTAAC 
TCCTCAGAGG ATTATATTCA TCGAATTGGA AGAACTGCTC GCAGTACCAA AACAGGCACA 
GCATACACTT TCTTTACTCC CAATAACATA AAGCAAGTGA GCGACCTCAT CTCTGTGCTT 
CGGGAAGCCA ATCAAGCAAT TAATCCCAAG TTGCTTCAGT TGGTCGAAGA CAGAGGTTCA 
GGTCGTTCCA GGGGTAGAGG AGGCATGAAG GATGATCGTC GTGACAGATA CTCTGCAGGG 
AAAAGGGGTG GATTTAATAC CTTTAGAGAC AGGGAAAACT ATGACAGAGG CTACTCTAGT 
CTGCTTAAGA GAGATTTTGG GGCTAAAACT CAGAATGGTG TTTACAGTGC TGCAAATTAC 
ACCAATGGGA GCTTTGGAAG TAATTTTGTA TCTGCTGGCA TACAGACCAG TTTTAGGACT 
GGTAACCCAA CAGGGACTTA CCAGAACGGT TATGATAGCA CTCAGCAATA TGGAAGTAAT 
GTTGCAAATA TGCACAATGG GATGAACCAA CAGGCATATG CATATCCTGC TACCGCAGCT 
GCTGCGCCTA TGATTGGCTA TCCAATGCCA ACAGGGTATT CTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766535.1
CDS	238..2085
Translation	MSSYSSDRDRGRDRGFGAPRFGGSRTGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVDTYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVANMHNGMNQQAYAYPATAAAAPMIGYPMPTGYSQ

Target ORF information:

RefSeq Version	XM_008768313.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 5 (Ddx5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768313.2

ORF Insert Sequence:

ATGTCGAGTT ATTCTAGTGA CCGAGACCGC GGCCGGGATC GAGGGTTTGG TGCACCTCGA 
TTTGGAGGGA GTAGAACAGG ACCCCTATCT GGAAAGAAGT TTGGAAATCC TGGGGAGAAA 
CTAGTTAAAA AGAAGTGGAA TCTTGATGAG CTGCCCAAAT TTGAGAAGAA TTTTTATCAG 
GAACACCCTG ATTTGGCAAG GCGCACCGCA CAAGAGGTAG ATACGTACAG AAGAAGCAAG 
GAAATTACAG TCAGAGGTCA CAACTGTCCA AAACCTGTTC TGAATTTTTA TGAAGCAAAC 
TTTCCTGCAA ATGTCATGGA TGTGATTGCA AGGCAGAACT TCACTGAACC CACTGCTATC 
CAAGCTCAGG GCTGGCCAGT TGCTCTCAGT GGATTGGATA TGGTTGGAGT AGCTCAGACT 
GGATCTGGGA AAACATTATC TTATTTGCTG CCTGCCATTG TACACATAAA CCACCAGCCA 
TTCTTAGAGA GAGGTGATGG GCCTATTTGC TTGGTGCTGG CACCAACTCG AGAACTGGCA 
CAGCAGGTGC AGCAGGTCGC TGCTGAATAT TGTAGAGCTT GTCGCTTGAA GTCTACTTGC 
ATCTATGGTG GTGCTCCCAA AGGACCGCAG ATTCGTGATT TGGAGAGAGG TGTGGAAATC 
TGTATTGCAA CACCTGGAAG ACTGATTGAC TTTTTAGAGT GTGGGAAAAC CAATCTGAGA 
AGAACAACTT ACCTTGTCCT TGATGAAGCT GATAGGATGC TTGATATGGG CTTTGAACCC 
CAAATAAGGA AAATTGTGGA TCAAATAAGA CCTGATAGGC AAACACTAAT GTGGAGTGCA 
ACTTGGCCAA AAGAAGTAAG ACAGCTTGCT GAAGATTTCC TGAAAGACTA TATTCATATC 
AATATTGGTG CACTGGAACT GAGTGCAAAC CATAACATTC TTCAGATTGT GGATGTGTGT 
CATGATGTCG AAAAGGATGA AAAACTTATT CGTCTGATGG AAGAGATCAT GAGTGAGAAG 
GAGAATAAAA CCATTGTTTT TGTTGAAACC AAAAGAAGAT GTGATGAACT TACCAGAAAA 
ATGAGGAGAG ATGGGTGGCC TGCCATGGGC ATCCATGGTG ACAAGAGTCA ACAGGAACGA 
GACTGGGTTC TAAATGAATT CAAACATGGA AAAGCTCCTA TTCTGATTGC TACCGATGTG 
GCCTCCAGAG GGCTAGATGT GGAAGATGTG AAATTTGTCA TCAATTATGA CTACCCTAAC 
TCCTCAGAGG ATTATATTCA TCGAATTGGA AGAACTGCTC GCAGTACCAA AACAGGCACA 
GCATACACTT TCTTTACTCC CAATAACATA AAGCAAGTGA GCGACCTCAT CTCTGTGCTT 
CGGGAAGCCA ATCAAGCAAT TAATCCCAAG TTGCTTCAGT TGGTCGAAGA CAGAGGTTCA 
GGTCGTTCCA GGGGTAGAGG AGGCATGAAG GATGATCGTC GTGACAGATA CTCTGCAGGG 
AAAAGGGGTG GATTTAATAC CTTTAGAGAC AGGGAAAACT ATGACAGAGG CTACTCTAGT 
CTGCTTAAGA GAGATTTTGG GGCTAAAACT CAGAATGGTG TTTACAGTGC TGCAAATTAC 
ACCAATGGGA GCTTTGGAAG TAATTTTGTA TCTGCTGGCA TACAGACCAG TTTTAGGACT 
GGTAACCCAA CAGGGACTTA CCAGAACGGT TATGATAGCA CTCAGCAATA TGGAAGTAAT 
GTTGCAAATA TGCACAATGG GATGAACCAA CAGGCATATG CATATCCTGC TACCGCAGCT 
GCTGCGCCTA TGATTGGCTA TCCAATGCCA ACAGGGTATT CTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Involvement of RNA helicase p68 in skin wound healing process in rats.
Chinese journal of traumatology = Zhonghua chuang shang za zhi20(6)311-317(2017 Dec)
Wang SJ,Du L,Shi CM

Macrophage migration inhibitory factor triggers vascular smooth muscle cell dedifferentiation by a p68-serum response factor axis.
Cardiovascular research113(5)519-530(2017 Apr)
Fan Y,Zhang J,Chen CY,Xiao YB,Asico LD,Jose PA,Xu JC,Qian GS,Zeng CY

JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.
Neuron88(6)1173-91(2015 Dec)
Berg JM,Lee C,Chen L,Galvan L,Cepeda C,Chen JY,Pe?agarikano O,Stein JL,Li A,Oguro-Ando A,Miller JA,Vashisht AA,Starks ME,Kite EP,Tam E,Gdalyahu A,Al-Sharif NB,Burkett ZD,White SA,Fears SC,Levine MS,Wohlschlegel JA,Geschwind DH

RNA helicases DDX5 and DDX17 dynamically orchestrate transcription, miRNA, and splicing programs in cell differentiation.
Cell reports7(6)1900-13(2014 Jun)
Dardenne E,Polay Espinoza M,Fattet L,Germann S,Lambert MP,Neil H,Zonta E,Mortada H,Gratadou L,Deygas M,Chakrama FZ,Samaan S,Desmet FO,Tranchevent LC,Dutertre M,Rimokh R,Bourgeois CF,Auboeuf D

The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways.
Nucleic acids research42(4)2197-207(2014 Feb)
Samaan S,Tranchevent LC,Dardenne E,Polay Espinoza M,Zonta E,Germann S,Gratadou L,Dutertre M,Auboeuf D

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Ddx5 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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