Wnk4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wnk4
Entrez Gene ID	287715
Full Name	WNK lysine deficient protein kinase 4
Synonyms	Ac2-059,Prkwnk4
General protein information	Preferred Names WNK lysine deficient protein kinase 4 Names serine/threonine-protein kinase WNK4 WNK4 Ser/Thr kinase protein kinase lysine-deficient 4 protein kinase with no lysine 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	mutation of the human homolog causes pseudohypoaldosteronism type II [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247279.3	XP_006247341.1	serine/threonine-protein kinase WNK4 isoform X2
XM_006247280.3	XP_006247342.1	serine/threonine-protein kinase WNK4 isoform X3
XM_006247281.3	XP_006247343.1	serine/threonine-protein kinase WNK4 isoform X4
XM_006247278.3	XP_006247340.1	serine/threonine-protein kinase WNK4 isoform X1
NM_175579.3	NP_783169.2	serine/threonine-protein kinase WNK4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC777580	XP_003642875.2
Pan troglodytes (chimpanzee)	WNK4	XP_003315535.1
Rattus norvegicus (Norway rat)	Wnk4	NP_783169.2
Oryza sativa (rice)	Os12g0162100	NP_001066222.1
Bos taurus (cattle)	WNK4	XP_003587489.1
Mus musculus (house mouse)	Wnk4	NP_783569.1
Oryza sativa (rice)	Os11g0160300	NP_001065816.1
Homo sapiens (human)	WNK4	NP_115763.2
	LOC707820	XP_002808177.1
Canis lupus familiaris (dog)	WNK4	XP_548082.3

Gene Ontology
Bibliography

Related articles in PubMed

WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats.
Bhuiyan MIH, Song S, Yuan H, Begum G, Kofler J, Kahle KT, Yang SS, Lin SH, Alper SL, Subramanya AR, Sun D
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism37(8)2780-2794(2017 Aug)

Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses.
Vorontsova I, Lam L, Delpire E, Lim J, Donaldson P
Investigative ophthalmology & visual science56(1)310-21(2014 Dec)

A novel Ste20-related proline/alanine-rich kinase (SPAK)-independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in the activation of Na-K-Cl cotransporters.
Ponce-Coria J, Markadieu N, Austin TM, Flammang L, Rios K, Welling PA, Delpire E
The Journal of biological chemistry289(25)17680-8(2014 Jun)

Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via ubiquitination and degradation of WNK4.
Shibata S, Zhang J, Puthumana J, Stone KL, Lifton RP
Proceedings of the National Academy of Sciences of the United States of America110(19)7838-43(2013 May)

Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension.
Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Matsuo H, Murata T, Nomura S, Asano T, Kawaguchi H, Nonoyama S, Rai T, Sasaki S, Uchida S
Cell reports3(3)858-68(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in addition to SPAK and OSR1, WNK4 is able to anchor itself to the N-terminal domain of NKCC1 and to promote cotransporter activation.
Title: A novel Ste20-related proline/alanine-rich kinase (SPAK)-independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in the activation of Na-K-Cl cotransporters.

results define a previously undescribed function of WNK4 and reveal a unique therapeutic target to control blood pressure in WNK4-related hypertension
Title: Serine-threonine kinase with-no-lysine 4 (WNK4) controls blood pressure via transient receptor potential canonical 3 (TRPC3) in the vasculature.

salt loading suppressed renal WNK4 expression, activated the Na(+)-Cl(-) cotransporter and induced salt-dependent hypertension
Title: Epigenetic modulation of the renal ?-adrenergic-WNK4 pathway in salt-sensitive hypertension.

Wnk4 phosphorylates ser(206) of claudin-7 and promotes paracellular Cl-ion permeability.
Title: WNK4 phosphorylates ser(206) of claudin-7 and promotes paracellular Cl(-) permeability.

WNK4 has a key role in coordinating the activities of diverse flux pathways to achieve integrated fluid and electrolyte homeostasis
Title: WNK4 regulates activity of the epithelial Na+ channel in vitro and in vivo.

The following Wnk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wnk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03791	NM_175579.3 Latest version!	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$888.30 $1269.00
ORa33660	XM_006247278.3 Latest version!	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa33661	XM_006247279.3 Latest version!	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa33662	XM_006247280.3 Latest version!	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa33663	XM_006247281.3 Latest version!	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wnk4 ( NM_175579.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_783169.2 Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), mRNA.

Price & Availability↑

CloneID	ORa03791
Clone ID Related Accession (Same CDS sequence)	NM_175579.3
Accession Version	NM_175579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-06
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase WNK4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY192567.1, AY187039.1 and BQ211864.1. On Oct 1, 2009 this sequence version replaced NM_175579.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY192567.1, AY187039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTAGCAC CTCGAAATAC AGAGACCGGG GTCCACATGT CCCAAACGGA GGCCGACCTG 
GCTTTGCGGC CATCTCCGTC TCTCACCTCC ATGGGGCCGA CCCGCCTGGG GCCACCTCCT 
CGCCGAGTGC GCCGCTTCTC TGGGAAGGCT GAGCCCCGGC CGCGCTCTTC GAGACCGAGC 
CGCCGCAGCT CAGTCGATCT GGGACTGTTG AGCTCCTGGT CTCAACCAGC CTCACTCCTT 
CCGGAACCTC CTGATCCTCC GGACTCCGCT GGTCCCATGA GGAGCCCACC TTCAAACTCT 
AAAGAACACC CCGAGGGCAC ATGGACGGGG GCAGCCCCGG TGAAGGCTGC AGACTCTGCC 
TGTCCTGAGC TTACAGTATC TTCCGGAGGT CCGGGGTCCC GGGAGCCACC AAGGGTTCCT 
GATGCTGCAG CCCGGGAACG GCGGCGGGAG CAGGAAGAAA AAGAGGACAC AGAGACCCAG 
GCTGTGGCAA CGTCTCCCGA CGGCCGATAC CTCAAGTTTG ACATTGAGAT TGGAAGAGGT 
TCCTTCAAGA CCGTGTATCG AGGGCTAGAC ACCGACACCA CGGTAGAGGT GGCCTGGTGT 
GAGCTGCAGG CTCGGAAACT CTCTCGGGCT GAACGGCAGC GTTTCTCTGA GGAAGTTGAG 
ATGCTCAAGG GGCTGCAGCA CCCCAACATC GTCCGCTTCT ATGACTCCTG GAAGTCGGTG 
CTGAGGGGTC AGGTTTGCAT CGTGCTGGTC ACAGAACTCA TGACCTCGGG CACGCTCAAG 
ACGTACCTGA GGAGATTCCG AGAAATGAAG CCCCGAGTCC TTCAGCGCTG GAGTCGCCAG 
ATCCTACGGG GACTTCATTT CTTACATTCC CGAGTGCCCC CCATCCTGCA CCGAGATCTC 
AAATGTGACA ATGTCTTCAT TACCGGCCCT TCAGGCTCTG TCAAAATCGG AGATCTCGGA 
CTGGCCACGC TCAAGCGCGC CTCCTTTGCC AAGAGCGTCA TTGGGACCCC GGAATTCATG 
GCCCCTGAGA TGTATGAGGA AAAGTACGAC GAGGCTGTGG ATGTGTACGC CTTTGGCATG 
TGCATGCTGG AGATGGCTAC GTCTGAGTAT CCCTACTCTG AGTGTCAGAA CGCAGCACAA 
ATCTACCGAA AGGTCACTTC GGGCACAAAG CCCAACAGCT TCTACAAAGT GAAGATGCCA 
GAGGTGAAGG AGATCATTGA AGGCTGCATC CGCACGGATA AGAATGAGAG GTTCACTATC 
CAGGATCTTC TGACCCACGC GTTCTTCCGC GAGGAACGTG GTGTGCATGT GGAGCTGGCA 
GAGGAAGATG ATGGAGAGAA GCCGGGTCTC AAACTCTGGC TGCGCATGGA GGACGCACGA 
CGCGGGGGGC GCCCAAGGGA CAACCAGGCC ATCGAGTTCC TGTTCCAGCT CGGTCGGGAT 
GCAGCGGAAG AGGTGGCTCA GGAGATGGTA GCCTTGGGCT TAGTCTGCGA AGCAGACTAC 
CAGCCAGTGG CCCGTGCAGT TCGAGAACGG GTTGCTGCCA TCCAGCGGAA GCGGGAGAAG 
CTAAGAAAAG CCAGGGAATT GGAGGTTCTC CCACCAGACT CAGGACCTCC TCCAGCAACT 
GTGTCTATGA CTCCTGGTCC CCCCAGTGCC TTCCCCCCAG AGCCCGAGGA GCCAGAGGCT 
GACCAGCACC AATCCTTCCT CTTCCGCCAT GCCAGCTACT CATCCACTAC ATCTGATTGC 
GAGACTGATG GCTACCTCAG CTCCTCCGGC TTCCTGGATG CCTCAGACCC TGCCCTTCAG 
CCCCCCGGGG GGATGCCATC CAGCCCCGCT GAGCCCCATC TCTGCTTGCC CTCGGGTTTT 
GCCTTGTCCA TTCCACGGTC TGGCCCTGGC AGTGACTTTT CTCCTGGGGA CAGCTACGCC 
TCAGATGCAG CCTCAGGCCT CAGTGACATG GGAGAAGGGG GGCAAATGAG GAAAAATCCA 
GTGAAGACTC TGCGACGGAG ACCTCGATCC CGGCTTCGGG TCACGAGTGT CTCAGACCAG 
AGCGACAGAG TCGTGGAGTG CCAGCTGCAA ACTCACAACA GCAAGATGGT GACGTTCCGA 
TTTGATCTGG ATGGGGACAG CCCAGAAGAA ATCGCAGCTG CCATGGTTTA TAATGAATTC 
ATTCTGCCCT CGGAGCGAGA CGGATTCCTG AGCCGGATCC GGGAGATTAT CCAGCGAGTG 
GAGACCCTGC TGAAGAGAGA TGCTGGTCCC TCGGAGGCCA CTGAAGATGC GCTGAGCCCT 
CAGGAAGAGG CAGCAGCCAT GCCTGCCCTC CCAGGCCCAT CCGATGAAGA GCTCCAGAGA 
AGCATCTCCC CGGAACAGAG GAGCTGGGCA GCCTTCTCTA CATCTCCATC TTCTCCTGGC 
ACCCCCTTGT CCCCTGGAAC CCCCTTTTCC CCTGGGACCC CTCCTGTCTT CCCATGCCCC 
ATCTTCCCTA TCACTTCACC CTCATGCCAT CCCTACCCGT TCTCACAGGT CTCTTCAAAT 
CCCTGTCCAC AGGCCCCCAG CTCCCTACTT CCCTCCTCCT CCGGTGCTTC TCAGGTTCCA 
TTCCCATCCC CTTCCCTTCC CACCAGCTCT CCCCTCCCAT TCTCTCCTAG TTATCCCCAA 
GTCCCTCTTC ACCCTGCTTC TCTTCCCACC TGCCCCTCCC CTCCCCCTCT CCCCTCCACC 
ACAGCAGCCC CTCTCCTCTC TCTGGCTAGT GCCTTCTCTC TGGCTGTGAT GACTGTGGCC 
CAGTCCCTGC TGTCCCCATC CCCTGGGCTT CTCTCTCAGT CTCCTCCAGC CCCTCCAGGT 
CCTCTGCCTA GCATGCCCCT TCCCCTTGCT TCTTGTGACC AGGAGAGCCT TTCAGCCCAA 
ACAGCTGAGA CAGAGAATGA GGCTTCCCGA AATCCTGCTC AGCCACTACT GGGTGATGCT 
AGACTGGCAC CCATATCTGA AGAGGGAAAA CCCCAGCTGG TTGGCCGATT CCAAGTGACT 
TCATCCAAGG AACCAGCAGA GCCTCCCCTA CAACCAGCAT CTCCAACTCT CTCCAGATCC 
CTAAAGCTTC CAACCCCTCA GCTGACCTCA GAGAGTTCAG ACACAGAGGA CAGTGCTGCA 
GGAGGACCAG AGACCAGGGA GGCTCTGGCA GAGAGTGACC GTGCAGCCGA GGGCTTGGGG 
GTCGCCATCG ATGAGGAAAA GGATGAAGGG AAGGAACCCC AAATTGGGGG CAGTTCCCCA 
ATCTTGAGCC AGCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTCTAAGC 
AGTGAGGAGT CAGAGAGCAG CGGGGAGGAC GAGGAGTTCT GGGCTGAGCT GCAGAACCTT 
CGGCAGAAGC ACTTGTCGGA AGTGGAAGCA CTACAGACGC TACAGAAGAA GGAAATCGAA 
GACTTGTACA GCCGGCTTGG AAAGCAACCC CCACCTGGTA TTGTAGCTCC AGCGGCTATG 
CTGTCCTGCC GCCAGCGCCG CCTCTCCAAG GGAAGCTTTC CCACCTCCCG CCGCAACAGC 
CTGCAGCGCT CTGATCTCCC TGGCCCTGGC ATCATGCGGA AGAACTCTCT GAGTGGCAGC 
AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG GGATGTTGGC 
AGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783169.2
CDS	27..3695
Translation	MLAPRNTETGVHMSQTEADLALRPSPSLTSMGPTRLGPPPRRVRRFSGKAEPRPRSSRPSRRSSVDLGLLSSWSQPASLLPEPPDPPDSAGPMRSPPSNSKEHPEGTWTGAAPVKAADSACPELTVSSGGPGSREPPRVPDAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQARKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIQDLLTHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSMTPGPPSAFPPEPEEPEADQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGMPSSPAEPHLCLPSGFALSIPRSGPGSDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPSEATEDALSPQEEAAAMPALPGPSDEELQRSISPEQRSWAAFSTSPSSPGTPLSPGTPFSPGTPPVFPCPIFPITSPSCHPYPFSQVSSNPCPQAPSSLLPSSSGASQVPFPSPSLPTSSPLPFSPSYPQVPLHPASLPTCPSPPPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSMPLPLASCDQESLSAQTAETENEASRNPAQPLLGDARLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPTPQLTSESSDTEDSAAGGPETREALAESDRAAEGLGVAIDEEKDEGKEPQIGGSSPILSQPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRKNSLSGSSTGSQEQRASKGVTFAGDVGRM

Target ORF information:

RefSeq Version	NM_175579.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175579.3

ORF Insert Sequence:

ATGCTAGCAC CTCGAAATAC AGAGACCGGG GTCCACATGT CCCAAACGGA GGCCGACCTG 
GCTTTGCGGC CATCTCCGTC TCTCACCTCC ATGGGGCCGA CCCGCCTGGG GCCACCTCCT 
CGCCGAGTGC GCCGCTTCTC TGGGAAGGCT GAGCCCCGGC CGCGCTCTTC GAGACCGAGC 
CGCCGCAGCT CAGTCGATCT GGGACTGTTG AGCTCCTGGT CTCAACCAGC CTCACTCCTT 
CCGGAACCTC CTGATCCTCC GGACTCCGCT GGTCCCATGA GGAGCCCACC TTCAAACTCT 
AAAGAACACC CCGAGGGCAC ATGGACGGGG GCAGCCCCGG TGAAGGCTGC AGACTCTGCC 
TGTCCTGAGC TTACAGTATC TTCCGGAGGT CCGGGGTCCC GGGAGCCACC AAGGGTTCCT 
GATGCTGCAG CCCGGGAACG GCGGCGGGAG CAGGAAGAAA AAGAGGACAC AGAGACCCAG 
GCTGTGGCAA CGTCTCCCGA CGGCCGATAC CTCAAGTTTG ACATTGAGAT TGGAAGAGGT 
TCCTTCAAGA CCGTGTATCG AGGGCTAGAC ACCGACACCA CGGTAGAGGT GGCCTGGTGT 
GAGCTGCAGG CTCGGAAACT CTCTCGGGCT GAACGGCAGC GTTTCTCTGA GGAAGTTGAG 
ATGCTCAAGG GGCTGCAGCA CCCCAACATC GTCCGCTTCT ATGACTCCTG GAAGTCGGTG 
CTGAGGGGTC AGGTTTGCAT CGTGCTGGTC ACAGAACTCA TGACCTCGGG CACGCTCAAG 
ACGTACCTGA GGAGATTCCG AGAAATGAAG CCCCGAGTCC TTCAGCGCTG GAGTCGCCAG 
ATCCTACGGG GACTTCATTT CTTACATTCC CGAGTGCCCC CCATCCTGCA CCGAGATCTC 
AAATGTGACA ATGTCTTCAT TACCGGCCCT TCAGGCTCTG TCAAAATCGG AGATCTCGGA 
CTGGCCACGC TCAAGCGCGC CTCCTTTGCC AAGAGCGTCA TTGGGACCCC GGAATTCATG 
GCCCCTGAGA TGTATGAGGA AAAGTACGAC GAGGCTGTGG ATGTGTACGC CTTTGGCATG 
TGCATGCTGG AGATGGCTAC GTCTGAGTAT CCCTACTCTG AGTGTCAGAA CGCAGCACAA 
ATCTACCGAA AGGTCACTTC GGGCACAAAG CCCAACAGCT TCTACAAAGT GAAGATGCCA 
GAGGTGAAGG AGATCATTGA AGGCTGCATC CGCACGGATA AGAATGAGAG GTTCACTATC 
CAGGATCTTC TGACCCACGC GTTCTTCCGC GAGGAACGTG GTGTGCATGT GGAGCTGGCA 
GAGGAAGATG ATGGAGAGAA GCCGGGTCTC AAACTCTGGC TGCGCATGGA GGACGCACGA 
CGCGGGGGGC GCCCAAGGGA CAACCAGGCC ATCGAGTTCC TGTTCCAGCT CGGTCGGGAT 
GCAGCGGAAG AGGTGGCTCA GGAGATGGTA GCCTTGGGCT TAGTCTGCGA AGCAGACTAC 
CAGCCAGTGG CCCGTGCAGT TCGAGAACGG GTTGCTGCCA TCCAGCGGAA GCGGGAGAAG 
CTAAGAAAAG CCAGGGAATT GGAGGTTCTC CCACCAGACT CAGGACCTCC TCCAGCAACT 
GTGTCTATGA CTCCTGGTCC CCCCAGTGCC TTCCCCCCAG AGCCCGAGGA GCCAGAGGCT 
GACCAGCACC AATCCTTCCT CTTCCGCCAT GCCAGCTACT CATCCACTAC ATCTGATTGC 
GAGACTGATG GCTACCTCAG CTCCTCCGGC TTCCTGGATG CCTCAGACCC TGCCCTTCAG 
CCCCCCGGGG GGATGCCATC CAGCCCCGCT GAGCCCCATC TCTGCTTGCC CTCGGGTTTT 
GCCTTGTCCA TTCCACGGTC TGGCCCTGGC AGTGACTTTT CTCCTGGGGA CAGCTACGCC 
TCAGATGCAG CCTCAGGCCT CAGTGACATG GGAGAAGGGG GGCAAATGAG GAAAAATCCA 
GTGAAGACTC TGCGACGGAG ACCTCGATCC CGGCTTCGGG TCACGAGTGT CTCAGACCAG 
AGCGACAGAG TCGTGGAGTG CCAGCTGCAA ACTCACAACA GCAAGATGGT GACGTTCCGA 
TTTGATCTGG ATGGGGACAG CCCAGAAGAA ATCGCAGCTG CCATGGTTTA TAATGAATTC 
ATTCTGCCCT CGGAGCGAGA CGGATTCCTG AGCCGGATCC GGGAGATTAT CCAGCGAGTG 
GAGACCCTGC TGAAGAGAGA TGCTGGTCCC TCGGAGGCCA CTGAAGATGC GCTGAGCCCT 
CAGGAAGAGG CAGCAGCCAT GCCTGCCCTC CCAGGCCCAT CCGATGAAGA GCTCCAGAGA 
AGCATCTCCC CGGAACAGAG GAGCTGGGCA GCCTTCTCTA CATCTCCATC TTCTCCTGGC 
ACCCCCTTGT CCCCTGGAAC CCCCTTTTCC CCTGGGACCC CTCCTGTCTT CCCATGCCCC 
ATCTTCCCTA TCACTTCACC CTCATGCCAT CCCTACCCGT TCTCACAGGT CTCTTCAAAT 
CCCTGTCCAC AGGCCCCCAG CTCCCTACTT CCCTCCTCCT CCGGTGCTTC TCAGGTTCCA 
TTCCCATCCC CTTCCCTTCC CACCAGCTCT CCCCTCCCAT TCTCTCCTAG TTATCCCCAA 
GTCCCTCTTC ACCCTGCTTC TCTTCCCACC TGCCCCTCCC CTCCCCCTCT CCCCTCCACC 
ACAGCAGCCC CTCTCCTCTC TCTGGCTAGT GCCTTCTCTC TGGCTGTGAT GACTGTGGCC 
CAGTCCCTGC TGTCCCCATC CCCTGGGCTT CTCTCTCAGT CTCCTCCAGC CCCTCCAGGT 
CCTCTGCCTA GCATGCCCCT TCCCCTTGCT TCTTGTGACC AGGAGAGCCT TTCAGCCCAA 
ACAGCTGAGA CAGAGAATGA GGCTTCCCGA AATCCTGCTC AGCCACTACT GGGTGATGCT 
AGACTGGCAC CCATATCTGA AGAGGGAAAA CCCCAGCTGG TTGGCCGATT CCAAGTGACT 
TCATCCAAGG AACCAGCAGA GCCTCCCCTA CAACCAGCAT CTCCAACTCT CTCCAGATCC 
CTAAAGCTTC CAACCCCTCA GCTGACCTCA GAGAGTTCAG ACACAGAGGA CAGTGCTGCA 
GGAGGACCAG AGACCAGGGA GGCTCTGGCA GAGAGTGACC GTGCAGCCGA GGGCTTGGGG 
GTCGCCATCG ATGAGGAAAA GGATGAAGGG AAGGAACCCC AAATTGGGGG CAGTTCCCCA 
ATCTTGAGCC AGCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTCTAAGC 
AGTGAGGAGT CAGAGAGCAG CGGGGAGGAC GAGGAGTTCT GGGCTGAGCT GCAGAACCTT 
CGGCAGAAGC ACTTGTCGGA AGTGGAAGCA CTACAGACGC TACAGAAGAA GGAAATCGAA 
GACTTGTACA GCCGGCTTGG AAAGCAACCC CCACCTGGTA TTGTAGCTCC AGCGGCTATG 
CTGTCCTGCC GCCAGCGCCG CCTCTCCAAG GGAAGCTTTC CCACCTCCCG CCGCAACAGC 
CTGCAGCGCT CTGATCTCCC TGGCCCTGGC ATCATGCGGA AGAACTCTCT GAGTGGCAGC 
AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG GGATGTTGGC 
AGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnk4 ( XM_006247278.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247340.1 Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33660
Clone ID Related Accession (Same CDS sequence)	XM_006247278.3
Accession Version	XM_006247278.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247278.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAC CTCGAAATAC AGAGACCGGG GTCCACATGT CCCAAACGGA GGCCGACCTG 
GCTTTGCGGC CATCTCCGTC TCTCACCTCC ATGGGGCCGA CCCGCCTGGG GCCACCTCCT 
CGCCGAGTGC GCCGCTTCTC TGGGAAGGCT GAGCCCCGGC CGCGCTCTTC GAGACCGAGC 
CGCCGCAGCT CAGTCGATCT GGGACTGTTG AGCTCCTGGT CTCAACCAGC CTCACTCCTT 
CCGGAACCTC CTGATCCTCC GGACTCCGCT GGTCCCATGA GGAGCCCACC TTCAAACTCT 
AAAGAACACC CCGAGGGCAC ATGGACGGGG GCAGCCCCGG TGAAGGCTGC AGACTCTGCC 
TGTCCTGAGC TTACAGTATC TTCCGGAGGT CCGGGGTCCC GGGAGCCACC AAGGGTTCCT 
GATGCTGCAG CCCGGGAACG GCGGCGGGAG CAGGAAGAAA AAGAGGACAC AGAGACCCAG 
GCTGTGGCAA CGTCTCCCGA CGGCCGATAC CTCAAGTTTG ACATTGAGAT TGGAAGAGGT 
TCCTTCAAGA CCGTGTATCG AGGGCTAGAC ACCGACACCA CGGTAGAGGT GGCCTGGTGT 
GAGCTGCAGA CTCGGAAACT CTCTCGGGCT GAACGGCAGC GTTTCTCTGA GGAAGTTGAG 
ATGCTCAAGG GGCTGCAGCA CCCCAACATC GTCCGCTTCT ATGACTCCTG GAAGTCGGTG 
CTGAGGGGTC AGGTTTGCAT CGTGCTGGTC ACAGAACTCA TGACCTCGGG CACGCTCAAG 
ACGTACCTGA GGAGATTCCG AGAAATGAAG CCCCGAGTCC TTCAGCGCTG GAGTCGCCAG 
ATCCTACGGG GACTTCATTT CTTACATTCC CGAGTGCCCC CCATCCTGCA CCGAGATCTC 
AAATGTGACA ATGTCTTCAT TACCGGCCCT TCAGGCTCTG TCAAAATCGG AGATCTCGGA 
CTGGCCACGC TCAAGCGCGC CTCCTTTGCC AAGAGCGTCA TTGGGACCCC GGAATTCATG 
GCCCCTGAGA TGTATGAGGA AAAGTACGAC GAGGCTGTGG ATGTGTACGC CTTTGGCATG 
TGCATGCTGG AGATGGCTAC GTCTGAGTAT CCCTACTCTG AGTGTCAGAA CGCAGCACAA 
ATCTACCGAA AGGTCACTTC GGGCACAAAG CCCAACAGCT TCTACAAAGT GAAGATGCCA 
GAGGTGAAGG AGATCATTGA AGGCTGCATC CGCACGGATA AGAATGAGAG GTTCACTATC 
CAGGATCTTC TGACCCACGC GTTCTTCCGC GAGGAACGTG GTGTGCATGT GGAGCTGGCA 
GAGGAAGATG ATGGAGAGAA GCCGGGTCTC AAACTCTGGC TGCGCATGGA GGACGCACGA 
CGCGGGGGGC GCCCAAGGGA CAACCAGGCC ATCGAGTTCC TGTTCCAGCT CGGTCGGGAT 
GCAGCGGAAG AGGTGGCTCA GGAGATGGTA GCCTTGGGCT TAGTCTGCGA AGCAGACTAC 
CAGCCAGTGG CCCGTGCAGT TCGAGAACGG GTTGCTGCCA TCCAGCGGAA GCGGGAGAAG 
CTAAGAAAAG CCAGGGAATT GGAGGTTCTC CCACCAGACT CAGGACCTCC TCCAGCAACT 
GTGTCTATGA CTCCTGGTCC CCCCAGTGCC TTCCCCCCAG AGCCCGAGGA GCCAGAGGCT 
GACCAGCACC AATCCTTCCT CTTCCGCCAT GCCAGCTACT CATCCACTAC ATCTGATTGC 
GAGACTGATG GCTACCTCAG CTCCTCCGGC TTCCTGGATG CCTCAGACCC TGCCCTTCAG 
CCCCCCGGGG GGATGCCATC CAGCCCCGCT GAGCCCCATC TCTGCTTGCC CTCGGGTTTT 
GCCTTGTCCA TTCCACGGTC TGGCCCTGGC AGTGACTTTT CTCCTGGGGA CAGCTACGCC 
TCAGATGCAG CCTCAGGCCT CAGTGACATG GGAGAAGGGG GGCAAATGAG GAAAAATCCA 
GTGAAGACTC TGCGACGGAG ACCTCGATCC CGGCTTCGGG TCACGAGTGT CTCAGACCAG 
AGCGACAGAG TCGTGGAGTG CCAGCTGCAA ACTCACAACA GCAAGATGGT GACGTTCCGA 
TTTGATCTGG ATGGGGACAG CCCAGAAGAA ATCGCAGCTG CCATGGTTTA TAATGAATTC 
ATTCTGCCCT CGGAGCGAGA CGGATTCCTG AGCCGGATCC GGGAGATTAT CCAGCGAGTG 
GAGACCCTGC TGAAGAGAGA TGCTGGTCCC TCGGAGGCCA CTGAAGATGC GCTGAGCCCT 
CAGGAAGAGC CAGCAGCCAT GCCTGCCCTC CCAGGCCCAT CCGATGCAGA GCTCCAGAGA 
AGCATCTCCC CGGAACAGAG GAGCTGGGCA GCCTTCTCTA CATCTCCATC TTCTCCTGGC 
ACCCCCTTGT CCCCTGGAAC CCCCTTTTCC CCTGGGACCC CTCCTGTCTT CCCATGCCCC 
ATCTTCCCTA TCACTTCACC CTCATGCCAT CCCTACCCGT TCTCACAGGT CTCTTCAAAT 
CCCTGTCCAC AGGCCCCCAG CTCCCTACTT CCCTCCTCCT CCGGTGCTTC TCAGGTTCCA 
TTCCCATCCC CTTCCCTTCC CACCAGCTCT CCCCTCCCAT TCTCTCCTAG TTATCCCCAA 
GTCCCTCTTC ACCCTGCTTC TCTTCCCACC TGCCCCTCCC CTCCCCCTCT CCCCTCCACC 
ACAGCAGCCC CTCTCCTCTC TCTGGCTAGT GCCTTCTCTC TGGCTGTGAT GACTGTGGCC 
CAGTCCCTGC TGTCCCCATC CCCTGGGCTT CTCTCTCAGT CTCCTCCAGC CCCTCCAGGT 
CCTCTGCCTA GCATGCCCCT TCCCCTTGCT TCTTGTGACC AGGAGAGCCT TTCAGCCCAA 
ACAGCTGAGA CAGAGAATGA GGCTTCCCGA AATCCTGCTC AGCCACTACT GGGTGATGCT 
AGACTGGCAC CCATATCTGA AGAGGGAAAA CCCCAGCTGG TTGGCCGATT CCAAGTGACT 
TCATCCAAGG AACCAGCAGA GCCTCCCCTA CAACCAGCAT CTCCAACTCT CTCCAGATCC 
CTAAAGCTTC CAACCCCTCA GCTGACCTCA GAGAGTTCAG ACACAGAGGA CAGTGCTGCA 
GGAGGACCAG AGACCAGGGA GGCTCTGGCA GAGAGTGACC GTGCAGCCGA GGGCTTGGGG 
GTCGCCATCG ATGAGGAAAA GGATGAAGGG AAGGAACCCC AAATTGGGGG CAGTTCCCCA 
ATCTTGAGCC AGCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTCTAAGC 
AGTGAGGAGT CAGAGAGCAG CGGGGAGGAC GAGGAGTTCT GGGCTGAGCT GCAGAACCTT 
CGGCAGAAGC ACTTGTCGGA AGTGGAAGCA CTACAGACGC TACAGAAGAA GGAAATCGAA 
GACTTGTACA GCCGGCTTGG AAAGCAACCC CCACCTGGTA TTGTAGCTCC AGCGGCTATG 
CTGTCCTGCC GCCAGCGCCG CCTCTCCAAG GGAAGCTTTC CCACCTCCCG CCGCAACAGC 
CTGCAGCGCT CTGATCTCCC TGGCCCTGGC ATCATGCGAA GGAACTCTCT GAGTGGCAGC 
AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG GGATGTTGGC 
AGGATGGTGA GGGGCGGGCC CAAGGGAGGG AGAGCTGAGG GAATGGTGGC TGGCAGCAGC 
TCTGCCTTCC TCTTCTTCGG ACATTTCTTC AGCACTTTTC TTTTCCCCTC CAGTGAATTC 
AGACCAGAAG TCCTATGTCT CCCCAACACC AGGGCCCCAC CATGGCCCGT GGCCTCTCAG 
AATCTGATGT TTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247340.1
CDS	141..3998
Translation	MLAPRNTETGVHMSQTEADLALRPSPSLTSMGPTRLGPPPRRVRRFSGKAEPRPRSSRPSRRSSVDLGLLSSWSQPASLLPEPPDPPDSAGPMRSPPSNSKEHPEGTWTGAAPVKAADSACPELTVSSGGPGSREPPRVPDAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIQDLLTHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSMTPGPPSAFPPEPEEPEADQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGMPSSPAEPHLCLPSGFALSIPRSGPGSDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPSEATEDALSPQEEPAAMPALPGPSDAELQRSISPEQRSWAAFSTSPSSPGTPLSPGTPFSPGTPPVFPCPIFPITSPSCHPYPFSQVSSNPCPQAPSSLLPSSSGASQVPFPSPSLPTSSPLPFSPSYPQVPLHPASLPTCPSPPPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSMPLPLASCDQESLSAQTAETENEASRNPAQPLLGDARLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPTPQLTSESSDTEDSAAGGPETREALAESDRAAEGLGVAIDEEKDEGKEPQIGGSSPILSQPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRMVRGGPKGGRAEGMVAGSSSAFLFFGHFFSTFLFPSSEFRPEVLCLPNTRAPPWPVASQNLMFL

Target ORF information:

RefSeq Version	XM_006247278.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247278.3

ORF Insert Sequence:

ATGCTAGCAC CTCGAAATAC AGAGACCGGG GTCCACATGT CCCAAACGGA GGCCGACCTG 
GCTTTGCGGC CATCTCCGTC TCTCACCTCC ATGGGGCCGA CCCGCCTGGG GCCACCTCCT 
CGCCGAGTGC GCCGCTTCTC TGGGAAGGCT GAGCCCCGGC CGCGCTCTTC GAGACCGAGC 
CGCCGCAGCT CAGTCGATCT GGGACTGTTG AGCTCCTGGT CTCAACCAGC CTCACTCCTT 
CCGGAACCTC CTGATCCTCC GGACTCCGCT GGTCCCATGA GGAGCCCACC TTCAAACTCT 
AAAGAACACC CCGAGGGCAC ATGGACGGGG GCAGCCCCGG TGAAGGCTGC AGACTCTGCC 
TGTCCTGAGC TTACAGTATC TTCCGGAGGT CCGGGGTCCC GGGAGCCACC AAGGGTTCCT 
GATGCTGCAG CCCGGGAACG GCGGCGGGAG CAGGAAGAAA AAGAGGACAC AGAGACCCAG 
GCTGTGGCAA CGTCTCCCGA CGGCCGATAC CTCAAGTTTG ACATTGAGAT TGGAAGAGGT 
TCCTTCAAGA CCGTGTATCG AGGGCTAGAC ACCGACACCA CGGTAGAGGT GGCCTGGTGT 
GAGCTGCAGA CTCGGAAACT CTCTCGGGCT GAACGGCAGC GTTTCTCTGA GGAAGTTGAG 
ATGCTCAAGG GGCTGCAGCA CCCCAACATC GTCCGCTTCT ATGACTCCTG GAAGTCGGTG 
CTGAGGGGTC AGGTTTGCAT CGTGCTGGTC ACAGAACTCA TGACCTCGGG CACGCTCAAG 
ACGTACCTGA GGAGATTCCG AGAAATGAAG CCCCGAGTCC TTCAGCGCTG GAGTCGCCAG 
ATCCTACGGG GACTTCATTT CTTACATTCC CGAGTGCCCC CCATCCTGCA CCGAGATCTC 
AAATGTGACA ATGTCTTCAT TACCGGCCCT TCAGGCTCTG TCAAAATCGG AGATCTCGGA 
CTGGCCACGC TCAAGCGCGC CTCCTTTGCC AAGAGCGTCA TTGGGACCCC GGAATTCATG 
GCCCCTGAGA TGTATGAGGA AAAGTACGAC GAGGCTGTGG ATGTGTACGC CTTTGGCATG 
TGCATGCTGG AGATGGCTAC GTCTGAGTAT CCCTACTCTG AGTGTCAGAA CGCAGCACAA 
ATCTACCGAA AGGTCACTTC GGGCACAAAG CCCAACAGCT TCTACAAAGT GAAGATGCCA 
GAGGTGAAGG AGATCATTGA AGGCTGCATC CGCACGGATA AGAATGAGAG GTTCACTATC 
CAGGATCTTC TGACCCACGC GTTCTTCCGC GAGGAACGTG GTGTGCATGT GGAGCTGGCA 
GAGGAAGATG ATGGAGAGAA GCCGGGTCTC AAACTCTGGC TGCGCATGGA GGACGCACGA 
CGCGGGGGGC GCCCAAGGGA CAACCAGGCC ATCGAGTTCC TGTTCCAGCT CGGTCGGGAT 
GCAGCGGAAG AGGTGGCTCA GGAGATGGTA GCCTTGGGCT TAGTCTGCGA AGCAGACTAC 
CAGCCAGTGG CCCGTGCAGT TCGAGAACGG GTTGCTGCCA TCCAGCGGAA GCGGGAGAAG 
CTAAGAAAAG CCAGGGAATT GGAGGTTCTC CCACCAGACT CAGGACCTCC TCCAGCAACT 
GTGTCTATGA CTCCTGGTCC CCCCAGTGCC TTCCCCCCAG AGCCCGAGGA GCCAGAGGCT 
GACCAGCACC AATCCTTCCT CTTCCGCCAT GCCAGCTACT CATCCACTAC ATCTGATTGC 
GAGACTGATG GCTACCTCAG CTCCTCCGGC TTCCTGGATG CCTCAGACCC TGCCCTTCAG 
CCCCCCGGGG GGATGCCATC CAGCCCCGCT GAGCCCCATC TCTGCTTGCC CTCGGGTTTT 
GCCTTGTCCA TTCCACGGTC TGGCCCTGGC AGTGACTTTT CTCCTGGGGA CAGCTACGCC 
TCAGATGCAG CCTCAGGCCT CAGTGACATG GGAGAAGGGG GGCAAATGAG GAAAAATCCA 
GTGAAGACTC TGCGACGGAG ACCTCGATCC CGGCTTCGGG TCACGAGTGT CTCAGACCAG 
AGCGACAGAG TCGTGGAGTG CCAGCTGCAA ACTCACAACA GCAAGATGGT GACGTTCCGA 
TTTGATCTGG ATGGGGACAG CCCAGAAGAA ATCGCAGCTG CCATGGTTTA TAATGAATTC 
ATTCTGCCCT CGGAGCGAGA CGGATTCCTG AGCCGGATCC GGGAGATTAT CCAGCGAGTG 
GAGACCCTGC TGAAGAGAGA TGCTGGTCCC TCGGAGGCCA CTGAAGATGC GCTGAGCCCT 
CAGGAAGAGC CAGCAGCCAT GCCTGCCCTC CCAGGCCCAT CCGATGCAGA GCTCCAGAGA 
AGCATCTCCC CGGAACAGAG GAGCTGGGCA GCCTTCTCTA CATCTCCATC TTCTCCTGGC 
ACCCCCTTGT CCCCTGGAAC CCCCTTTTCC CCTGGGACCC CTCCTGTCTT CCCATGCCCC 
ATCTTCCCTA TCACTTCACC CTCATGCCAT CCCTACCCGT TCTCACAGGT CTCTTCAAAT 
CCCTGTCCAC AGGCCCCCAG CTCCCTACTT CCCTCCTCCT CCGGTGCTTC TCAGGTTCCA 
TTCCCATCCC CTTCCCTTCC CACCAGCTCT CCCCTCCCAT TCTCTCCTAG TTATCCCCAA 
GTCCCTCTTC ACCCTGCTTC TCTTCCCACC TGCCCCTCCC CTCCCCCTCT CCCCTCCACC 
ACAGCAGCCC CTCTCCTCTC TCTGGCTAGT GCCTTCTCTC TGGCTGTGAT GACTGTGGCC 
CAGTCCCTGC TGTCCCCATC CCCTGGGCTT CTCTCTCAGT CTCCTCCAGC CCCTCCAGGT 
CCTCTGCCTA GCATGCCCCT TCCCCTTGCT TCTTGTGACC AGGAGAGCCT TTCAGCCCAA 
ACAGCTGAGA CAGAGAATGA GGCTTCCCGA AATCCTGCTC AGCCACTACT GGGTGATGCT 
AGACTGGCAC CCATATCTGA AGAGGGAAAA CCCCAGCTGG TTGGCCGATT CCAAGTGACT 
TCATCCAAGG AACCAGCAGA GCCTCCCCTA CAACCAGCAT CTCCAACTCT CTCCAGATCC 
CTAAAGCTTC CAACCCCTCA GCTGACCTCA GAGAGTTCAG ACACAGAGGA CAGTGCTGCA 
GGAGGACCAG AGACCAGGGA GGCTCTGGCA GAGAGTGACC GTGCAGCCGA GGGCTTGGGG 
GTCGCCATCG ATGAGGAAAA GGATGAAGGG AAGGAACCCC AAATTGGGGG CAGTTCCCCA 
ATCTTGAGCC AGCCCAGCCC AGTGTGGATG AACTACTCCT ACAGCAGCCT GTGTCTAAGC 
AGTGAGGAGT CAGAGAGCAG CGGGGAGGAC GAGGAGTTCT GGGCTGAGCT GCAGAACCTT 
CGGCAGAAGC ACTTGTCGGA AGTGGAAGCA CTACAGACGC TACAGAAGAA GGAAATCGAA 
GACTTGTACA GCCGGCTTGG AAAGCAACCC CCACCTGGTA TTGTAGCTCC AGCGGCTATG 
CTGTCCTGCC GCCAGCGCCG CCTCTCCAAG GGAAGCTTTC CCACCTCCCG CCGCAACAGC 
CTGCAGCGCT CTGATCTCCC TGGCCCTGGC ATCATGCGAA GGAACTCTCT GAGTGGCAGC 
AGCACCGGCT CCCAGGAGCA GCGGGCAAGC AAGGGGGTGA CATTCGCCGG GGATGTTGGC 
AGGATGGTGA GGGGCGGGCC CAAGGGAGGG AGAGCTGAGG GAATGGTGGC TGGCAGCAGC 
TCTGCCTTCC TCTTCTTCGG ACATTTCTTC AGCACTTTTC TTTTCCCCTC CAGTGAATTC 
AGACCAGAAG TCCTATGTCT CCCCAACACC AGGGCCCCAC CATGGCCCGT GGCCTCTCAG 
AATCTGATGT TTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnk4 ( XM_006247279.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247341.1 Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X2, mRNA.

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CloneID	ORa33661
Clone ID Related Accession (Same CDS sequence)	XM_006247279.3
Accession Version	XM_006247279.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247279.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGCAC CTCGAAATAC AGAGACCGGG GTCCACATGT CCCAAACGGA GGCCGACCTG 
GCTTTGCGGC CATCTCCGTC TCTCACCTCC ATGGGGCCGA CCCGCCTGGG GCCACCTCCT 
CGCCGAGTGC GCCGCTTCTC TGGGAAGGCT GAGCCCCGGC CGCGCTCTTC GAGACCGAGC 
CGCCGCAGCT CAGTCGATCT GGGACTGTTG AGCTCCTGGT CTCAACCAGC CTCACTCCTT 
CCGGAACCTC CTGATCCTCC GGACTCCGCT GGTCCCATGA GGAGCCCACC TTCAAACTCT 
AAAGAACACC CCGAGGGCAC ATGGACGGGG GCAGCCCCGG TGAAGGCTGC AGACTCTGCC 
TGTCCTGAGC TTACAGTATC TTCCGGAGGT CCGGGGTCCC GGGAGCCACC AAGGGTTCCT 
GATGCTGCAG CCCGGGAACG GCGGCGGGAG CAGGAAGAAA AAGAGGACAC AGAGACCCAG 
GCTGTGGCAA CGTCTCCCGA CGGCCGATAC CTCAAGTTTG ACATTGAGAT TGGAAGAGGT 
TCCTTCAAGA CCGTGTATCG AGGGCTAGAC ACCGACACCA CGGTAGAGGT GGCCTGGTGT 
GAGCTGCAGA CTCGGAAACT CTCTCGGGCT GAACGGCAGC GTTTCTCTGA GGAAGTTGAG 
ATGCTCAAGG GGCTGCAGCA CCCCAACATC GTCCGCTTCT ATGACTCCTG GAAGTCGGTG 
CTGAGGGGTC AGGTTTGCAT CGTGCTGGTC ACAGAACTCA TGACCTCGGG CACGCTCAAG 
ACGTACCTGA GGAGATTCCG AGAAATGAAG CCCCGAGTCC TTCAGCGCTG GAGTCGCCAG 
ATCCTACGGG GACTTCATTT CTTACATTCC CGAGTGCCCC CCATCCTGCA CCGAGATCTC 
AAATGTGACA ATGTCTTCAT TACCGGCCCT TCAGGCTCTG TCAAAATCGG AGATCTCGGA 
CTGGCCACGC TCAAGCGCGC CTCCTTTGCC AAGAGCGTCA TTGGGACCCC GGAATTCATG 
GCCCCTGAGA TGTATGAGGA AAAGTACGAC GAGGCTGTGG ATGTGTACGC CTTTGGCATG 
TGCATGCTGG AGATGGCTAC GTCTGAGTAT CCCTACTCTG AGTGTCAGAA CGCAGCACAA 
ATCTACCGAA AGGTCACTTC GGGCACAAAG CCCAACAGCT TCTACAAAGT GAAGATGCCA 
GAGGTGAAGG AGATCATTGA AGGCTGCATC CGCACGGATA AGAATGAGAG GTTCACTATC 
CAGGATCTTC TGACCCACGC GTTCTTCCGC GAGGAACGTG GTGTGCATGT GGAGCTGGCA 
GAGGAAGATG ATGGAGAGAA GCCGGGTCTC AAACTCTGGC TGCGCATGGA GGACGCACGA 
CGCGGGGGGC GCCCAAGGGA CAACCAGGCC ATCGAGTTCC TGTTCCAGCT CGGTCGGGAT 
GCAGCGGAAG AGGTGGCTCA GGAGATGGTA GCCTTGGGCT TAGTCTGCGA AGCAGACTAC 
CAGCCAGTGG CCCGTGCAGT TCGAGAACGG GTTGCTGCCA TCCAGCGGAA GCGGGAGAAG 
CTAAGAAAAG CCAGGGAATT GGAGGTTCTC CCACCAGACT CAGGACCTCC TCCAGCAACT 
GTGTCTATGA CTCCTGGTCC CCCCAGTGCC TTCCCCCCAG AGCCCGAGGA GCCAGAGGCT 
GACCAGCACC AATCCTTCCT CTTCCGCCAT GCCAGCTACT CATCCACTAC ATCTGATTGC 
GAGACTGATG GCTACCTCAG CTCCTCCGGC TTCCTGGATG CCTCAGACCC TGCCCTTCAG 
CCCCCCGGGG GGATGCCATC CAGCCCCGCT GAGCCCCATC TCTGCTTGCC CTCGGGTTTT 
GCCTTGTCCA TTCCACGGTC TGGCCCTGGC AGTGACTTTT CTCCTGGGGA CAGCTACGCC 
TCAGATGCAG CCTCAGGCCT CAGTGACATG GGAGAAGGGG GGCAAATGAG GAAAAATCCA 
GTGAAGACTC TGCGACGGAG ACCTCGATCC CGGCTTCGGG TCACGAGTGT CTCAGACCAG 
AGCGACAGAG TCGTGGAGTG CCAGCTGCAA ACTCACAACA GCAAGATGGT GACGTTCCGA 
TTTGATCTGG ATGGGGACAG CCCAGAAGAA ATCGCAGCTG CCATGGTTTA TAATGAATTC 
ATTCTGCCCT CGGAGCGAGA CGGATTCCTG AGCCGGATCC GGGAGATTAT CCAGCGAGTG 
GAGACCCTGC TGAAGAGAGA TGCTGGTCCC TCGGAGGCCA CTGAAGATGC GCTGAGCCCT 
CAGGAAGAGC CAGCAGCCAT GCCTGCCCTC CCAGGCCCAT CCGATGAGCT CCAGAGAAGC 
ATCTCCCCGG AACAGAGGAG CTGGGCAGCC TTCTCTACAT CTCCATCTTC TCCTGGCACC 
CCCTTGTCCC CTGGAACCCC CTTTTCCCCT GGGACCCCTC CTGTCTTCCC ATGCCCCATC 
TTCCCTATCA CTTCACCCTC ATGCCATCCC TACCCGTTCT CACAGGTCTC TTCAAATCCC 
TGTCCACAGG CCCCCAGCTC CCTACTTCCC TCCTCCTCCG GTGCTTCTCA GGTTCCATTC 
CCATCCCCTT CCCTTCCCAC CAGCTCTCCC CTCCCATTCT CTCCTAGTTA TCCCCAAGTC 
CCTCTTCACC CTGCTTCTCT TCCCACCTGC CCCTCCCCTC CCCCTCTCCC CTCCACCACA 
GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC TTCTCTCTGG CTGTGATGAC TGTGGCCCAG 
TCCCTGCTGT CCCCATCCCC TGGGCTTCTC TCTCAGTCTC CTCCAGCCCC TCCAGGTCCT 
CTGCCTAGCA TGCCCCTTCC CCTTGCTTCT TGTGACCAGG AGAGCCTTTC AGCCCAAACA 
GCTGAGACAG AGAATGAGGC TTCCCGAAAT CCTGCTCAGC CACTACTGGG TGATGCTAGA 
CTGGCACCCA TATCTGAAGA GGGAAAACCC CAGCTGGTTG GCCGATTCCA AGTGACTTCA 
TCCAAGGAAC CAGCAGAGCC TCCCCTACAA CCAGCATCTC CAACTCTCTC CAGATCCCTA 
AAGCTTCCAA CCCCTCAGCT GACCTCAGAG AGTTCAGACA CAGAGGACAG TGCTGCAGGA 
GGACCAGAGA CCAGGGAGGC TCTGGCAGAG AGTGACCGTG CAGCCGAGGG CTTGGGGGTC 
GCCATCGATG AGGAAAAGGA TGAAGGGAAG GAACCCCAAA TTGGGGGCAG TTCCCCAATC 
TTGAGCCAGC CCAGCCCAGT GTGGATGAAC TACTCCTACA GCAGCCTGTG TCTAAGCAGT 
GAGGAGTCAG AGAGCAGCGG GGAGGACGAG GAGTTCTGGG CTGAGCTGCA GAACCTTCGG 
CAGAAGCACT TGTCGGAAGT GGAAGCACTA CAGACGCTAC AGAAGAAGGA AATCGAAGAC 
TTGTACAGCC GGCTTGGAAA GCAACCCCCA CCTGGTATTG TAGCTCCAGC GGCTATGCTG 
TCCTGCCGCC AGCGCCGCCT CTCCAAGGGA AGCTTTCCCA CCTCCCGCCG CAACAGCCTG 
CAGCGCTCTG ATCTCCCTGG CCCTGGCATC ATGCGAAGGA ACTCTCTGAG TGGCAGCAGC 
ACCGGCTCCC AGGAGCAGCG GGCAAGCAAG GGGGTGACAT TCGCCGGGGA TGTTGGCAGG 
ATGGTGAGGG GCGGGCCCAA GGGAGGGAGA GCTGAGGGAA TGGTGGCTGG CAGCAGCTCT 
GCCTTCCTCT TCTTCGGACA TTTCTTCAGC ACTTTTCTTT TCCCCTCCAG TGAATTCAGA 
CCAGAAGTCC TATGTCTCCC CAACACCAGG GCCCCACCAT GGCCCGTGGC CTCTCAGAAT 
CTGATGTTTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247341.1
CDS	141..3995
Translation	MLAPRNTETGVHMSQTEADLALRPSPSLTSMGPTRLGPPPRRVRRFSGKAEPRPRSSRPSRRSSVDLGLLSSWSQPASLLPEPPDPPDSAGPMRSPPSNSKEHPEGTWTGAAPVKAADSACPELTVSSGGPGSREPPRVPDAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIQDLLTHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSMTPGPPSAFPPEPEEPEADQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGMPSSPAEPHLCLPSGFALSIPRSGPGSDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPSEATEDALSPQEEPAAMPALPGPSDELQRSISPEQRSWAAFSTSPSSPGTPLSPGTPFSPGTPPVFPCPIFPITSPSCHPYPFSQVSSNPCPQAPSSLLPSSSGASQVPFPSPSLPTSSPLPFSPSYPQVPLHPASLPTCPSPPPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSMPLPLASCDQESLSAQTAETENEASRNPAQPLLGDARLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPTPQLTSESSDTEDSAAGGPETREALAESDRAAEGLGVAIDEEKDEGKEPQIGGSSPILSQPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRMVRGGPKGGRAEGMVAGSSSAFLFFGHFFSTFLFPSSEFRPEVLCLPNTRAPPWPVASQNLMFL

Target ORF information:

RefSeq Version	XM_006247279.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247279.3

ORF Insert Sequence:

ATGCTAGCAC CTCGAAATAC AGAGACCGGG GTCCACATGT CCCAAACGGA GGCCGACCTG 
GCTTTGCGGC CATCTCCGTC TCTCACCTCC ATGGGGCCGA CCCGCCTGGG GCCACCTCCT 
CGCCGAGTGC GCCGCTTCTC TGGGAAGGCT GAGCCCCGGC CGCGCTCTTC GAGACCGAGC 
CGCCGCAGCT CAGTCGATCT GGGACTGTTG AGCTCCTGGT CTCAACCAGC CTCACTCCTT 
CCGGAACCTC CTGATCCTCC GGACTCCGCT GGTCCCATGA GGAGCCCACC TTCAAACTCT 
AAAGAACACC CCGAGGGCAC ATGGACGGGG GCAGCCCCGG TGAAGGCTGC AGACTCTGCC 
TGTCCTGAGC TTACAGTATC TTCCGGAGGT CCGGGGTCCC GGGAGCCACC AAGGGTTCCT 
GATGCTGCAG CCCGGGAACG GCGGCGGGAG CAGGAAGAAA AAGAGGACAC AGAGACCCAG 
GCTGTGGCAA CGTCTCCCGA CGGCCGATAC CTCAAGTTTG ACATTGAGAT TGGAAGAGGT 
TCCTTCAAGA CCGTGTATCG AGGGCTAGAC ACCGACACCA CGGTAGAGGT GGCCTGGTGT 
GAGCTGCAGA CTCGGAAACT CTCTCGGGCT GAACGGCAGC GTTTCTCTGA GGAAGTTGAG 
ATGCTCAAGG GGCTGCAGCA CCCCAACATC GTCCGCTTCT ATGACTCCTG GAAGTCGGTG 
CTGAGGGGTC AGGTTTGCAT CGTGCTGGTC ACAGAACTCA TGACCTCGGG CACGCTCAAG 
ACGTACCTGA GGAGATTCCG AGAAATGAAG CCCCGAGTCC TTCAGCGCTG GAGTCGCCAG 
ATCCTACGGG GACTTCATTT CTTACATTCC CGAGTGCCCC CCATCCTGCA CCGAGATCTC 
AAATGTGACA ATGTCTTCAT TACCGGCCCT TCAGGCTCTG TCAAAATCGG AGATCTCGGA 
CTGGCCACGC TCAAGCGCGC CTCCTTTGCC AAGAGCGTCA TTGGGACCCC GGAATTCATG 
GCCCCTGAGA TGTATGAGGA AAAGTACGAC GAGGCTGTGG ATGTGTACGC CTTTGGCATG 
TGCATGCTGG AGATGGCTAC GTCTGAGTAT CCCTACTCTG AGTGTCAGAA CGCAGCACAA 
ATCTACCGAA AGGTCACTTC GGGCACAAAG CCCAACAGCT TCTACAAAGT GAAGATGCCA 
GAGGTGAAGG AGATCATTGA AGGCTGCATC CGCACGGATA AGAATGAGAG GTTCACTATC 
CAGGATCTTC TGACCCACGC GTTCTTCCGC GAGGAACGTG GTGTGCATGT GGAGCTGGCA 
GAGGAAGATG ATGGAGAGAA GCCGGGTCTC AAACTCTGGC TGCGCATGGA GGACGCACGA 
CGCGGGGGGC GCCCAAGGGA CAACCAGGCC ATCGAGTTCC TGTTCCAGCT CGGTCGGGAT 
GCAGCGGAAG AGGTGGCTCA GGAGATGGTA GCCTTGGGCT TAGTCTGCGA AGCAGACTAC 
CAGCCAGTGG CCCGTGCAGT TCGAGAACGG GTTGCTGCCA TCCAGCGGAA GCGGGAGAAG 
CTAAGAAAAG CCAGGGAATT GGAGGTTCTC CCACCAGACT CAGGACCTCC TCCAGCAACT 
GTGTCTATGA CTCCTGGTCC CCCCAGTGCC TTCCCCCCAG AGCCCGAGGA GCCAGAGGCT 
GACCAGCACC AATCCTTCCT CTTCCGCCAT GCCAGCTACT CATCCACTAC ATCTGATTGC 
GAGACTGATG GCTACCTCAG CTCCTCCGGC TTCCTGGATG CCTCAGACCC TGCCCTTCAG 
CCCCCCGGGG GGATGCCATC CAGCCCCGCT GAGCCCCATC TCTGCTTGCC CTCGGGTTTT 
GCCTTGTCCA TTCCACGGTC TGGCCCTGGC AGTGACTTTT CTCCTGGGGA CAGCTACGCC 
TCAGATGCAG CCTCAGGCCT CAGTGACATG GGAGAAGGGG GGCAAATGAG GAAAAATCCA 
GTGAAGACTC TGCGACGGAG ACCTCGATCC CGGCTTCGGG TCACGAGTGT CTCAGACCAG 
AGCGACAGAG TCGTGGAGTG CCAGCTGCAA ACTCACAACA GCAAGATGGT GACGTTCCGA 
TTTGATCTGG ATGGGGACAG CCCAGAAGAA ATCGCAGCTG CCATGGTTTA TAATGAATTC 
ATTCTGCCCT CGGAGCGAGA CGGATTCCTG AGCCGGATCC GGGAGATTAT CCAGCGAGTG 
GAGACCCTGC TGAAGAGAGA TGCTGGTCCC TCGGAGGCCA CTGAAGATGC GCTGAGCCCT 
CAGGAAGAGC CAGCAGCCAT GCCTGCCCTC CCAGGCCCAT CCGATGAGCT CCAGAGAAGC 
ATCTCCCCGG AACAGAGGAG CTGGGCAGCC TTCTCTACAT CTCCATCTTC TCCTGGCACC 
CCCTTGTCCC CTGGAACCCC CTTTTCCCCT GGGACCCCTC CTGTCTTCCC ATGCCCCATC 
TTCCCTATCA CTTCACCCTC ATGCCATCCC TACCCGTTCT CACAGGTCTC TTCAAATCCC 
TGTCCACAGG CCCCCAGCTC CCTACTTCCC TCCTCCTCCG GTGCTTCTCA GGTTCCATTC 
CCATCCCCTT CCCTTCCCAC CAGCTCTCCC CTCCCATTCT CTCCTAGTTA TCCCCAAGTC 
CCTCTTCACC CTGCTTCTCT TCCCACCTGC CCCTCCCCTC CCCCTCTCCC CTCCACCACA 
GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC TTCTCTCTGG CTGTGATGAC TGTGGCCCAG 
TCCCTGCTGT CCCCATCCCC TGGGCTTCTC TCTCAGTCTC CTCCAGCCCC TCCAGGTCCT 
CTGCCTAGCA TGCCCCTTCC CCTTGCTTCT TGTGACCAGG AGAGCCTTTC AGCCCAAACA 
GCTGAGACAG AGAATGAGGC TTCCCGAAAT CCTGCTCAGC CACTACTGGG TGATGCTAGA 
CTGGCACCCA TATCTGAAGA GGGAAAACCC CAGCTGGTTG GCCGATTCCA AGTGACTTCA 
TCCAAGGAAC CAGCAGAGCC TCCCCTACAA CCAGCATCTC CAACTCTCTC CAGATCCCTA 
AAGCTTCCAA CCCCTCAGCT GACCTCAGAG AGTTCAGACA CAGAGGACAG TGCTGCAGGA 
GGACCAGAGA CCAGGGAGGC TCTGGCAGAG AGTGACCGTG CAGCCGAGGG CTTGGGGGTC 
GCCATCGATG AGGAAAAGGA TGAAGGGAAG GAACCCCAAA TTGGGGGCAG TTCCCCAATC 
TTGAGCCAGC CCAGCCCAGT GTGGATGAAC TACTCCTACA GCAGCCTGTG TCTAAGCAGT 
GAGGAGTCAG AGAGCAGCGG GGAGGACGAG GAGTTCTGGG CTGAGCTGCA GAACCTTCGG 
CAGAAGCACT TGTCGGAAGT GGAAGCACTA CAGACGCTAC AGAAGAAGGA AATCGAAGAC 
TTGTACAGCC GGCTTGGAAA GCAACCCCCA CCTGGTATTG TAGCTCCAGC GGCTATGCTG 
TCCTGCCGCC AGCGCCGCCT CTCCAAGGGA AGCTTTCCCA CCTCCCGCCG CAACAGCCTG 
CAGCGCTCTG ATCTCCCTGG CCCTGGCATC ATGCGAAGGA ACTCTCTGAG TGGCAGCAGC 
ACCGGCTCCC AGGAGCAGCG GGCAAGCAAG GGGGTGACAT TCGCCGGGGA TGTTGGCAGG 
ATGGTGAGGG GCGGGCCCAA GGGAGGGAGA GCTGAGGGAA TGGTGGCTGG CAGCAGCTCT 
GCCTTCCTCT TCTTCGGACA TTTCTTCAGC ACTTTTCTTT TCCCCTCCAG TGAATTCAGA 
CCAGAAGTCC TATGTCTCCC CAACACCAGG GCCCCACCAT GGCCCGTGGC CTCTCAGAAT 
CTGATGTTTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnk4 ( XM_006247280.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247342.1 Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa33662
Clone ID Related Accession (Same CDS sequence)	XM_006247280.3
Accession Version	XM_006247280.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase WNK4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247280.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGATTG GCAGTTTCTT CCTCCCGTGT CTCCCACTGT TATGTACTCG GAAACTCTCT 
CGGGCTGAAC GGCAGCGTTT CTCTGAGGAA GTTGAGATGC TCAAGGGGCT GCAGCACCCC 
AACATCGTCC GCTTCTATGA CTCCTGGAAG TCGGTGCTGA GGGGTCAGGT TTGCATCGTG 
CTGGTCACAG AACTCATGAC CTCGGGCACG CTCAAGACGT ACCTGAGGAG ATTCCGAGAA 
ATGAAGCCCC GAGTCCTTCA GCGCTGGAGT CGCCAGATCC TACGGGGACT TCATTTCTTA 
CATTCCCGAG TGCCCCCCAT CCTGCACCGA GATCTCAAAT GTGACAATGT CTTCATTACC 
GGCCCTTCAG GCTCTGTCAA AATCGGAGAT CTCGGACTGG CCACGCTCAA GCGCGCCTCC 
TTTGCCAAGA GCGTCATTGG GACCCCGGAA TTCATGGCCC CTGAGATGTA TGAGGAAAAG 
TACGACGAGG CTGTGGATGT GTACGCCTTT GGCATGTGCA TGCTGGAGAT GGCTACGTCT 
GAGTATCCCT ACTCTGAGTG TCAGAACGCA GCACAAATCT ACCGAAAGGT CACTTCGGGC 
ACAAAGCCCA ACAGCTTCTA CAAAGTGAAG ATGCCAGAGG TGAAGGAGAT CATTGAAGGC 
TGCATCCGCA CGGATAAGAA TGAGAGGTTC ACTATCCAGG ATCTTCTGAC CCACGCGTTC 
TTCCGCGAGG AACGTGGTGT GCATGTGGAG CTGGCAGAGG AAGATGATGG AGAGAAGCCG 
GGTCTCAAAC TCTGGCTGCG CATGGAGGAC GCACGACGCG GGGGGCGCCC AAGGGACAAC 
CAGGCCATCG AGTTCCTGTT CCAGCTCGGT CGGGATGCAG CGGAAGAGGT GGCTCAGGAG 
ATGGTAGCCT TGGGCTTAGT CTGCGAAGCA GACTACCAGC CAGTGGCCCG TGCAGTTCGA 
GAACGGGTTG CTGCCATCCA GCGGAAGCGG GAGAAGCTAA GAAAAGCCAG GGAATTGGAG 
GTTCTCCCAC CAGACTCAGG ACCTCCTCCA GCAACTGTGT CTATGACTCC TGGTCCCCCC 
AGTGCCTTCC CCCCAGAGCC CGAGGAGCCA GAGGCTGACC AGCACCAATC CTTCCTCTTC 
CGCCATGCCA GCTACTCATC CACTACATCT GATTGCGAGA CTGATGGCTA CCTCAGCTCC 
TCCGGCTTCC TGGATGCCTC AGACCCTGCC CTTCAGCCCC CCGGGGGGAT GCCATCCAGC 
CCCGCTGAGC CCCATCTCTG CTTGCCCTCG GGTTTTGCCT TGTCCATTCC ACGGTCTGGC 
CCTGGCAGTG ACTTTTCTCC TGGGGACAGC TACGCCTCAG ATGCAGCCTC AGGCCTCAGT 
GACATGGGAG AAGGGGGGCA AATGAGGAAA AATCCAGTGA AGACTCTGCG ACGGAGACCT 
CGATCCCGGC TTCGGGTCAC GAGTGTCTCA GACCAGAGCG ACAGAGTCGT GGAGTGCCAG 
CTGCAAACTC ACAACAGCAA GATGGTGACG TTCCGATTTG ATCTGGATGG GGACAGCCCA 
GAAGAAATCG CAGCTGCCAT GGTTTATAAT GAATTCATTC TGCCCTCGGA GCGAGACGGA 
TTCCTGAGCC GGATCCGGGA GATTATCCAG CGAGTGGAGA CCCTGCTGAA GAGAGATGCT 
GGTCCCTCGG AGGCCACTGA AGATGCGCTG AGCCCTCAGG AAGAGCCAGC AGCCATGCCT 
GCCCTCCCAG GCCCATCCGA TGCAGAGCTC CAGAGAAGCA TCTCCCCGGA ACAGAGGAGC 
TGGGCAGCCT TCTCTACATC TCCATCTTCT CCTGGCACCC CCTTGTCCCC TGGAACCCCC 
TTTTCCCCTG GGACCCCTCC TGTCTTCCCA TGCCCCATCT TCCCTATCAC TTCACCCTCA 
TGCCATCCCT ACCCGTTCTC ACAGGTCTCT TCAAATCCCT GTCCACAGGC CCCCAGCTCC 
CTACTTCCCT CCTCCTCCGG TGCTTCTCAG GTTCCATTCC CATCCCCTTC CCTTCCCACC 
AGCTCTCCCC TCCCATTCTC TCCTAGTTAT CCCCAAGTCC CTCTTCACCC TGCTTCTCTT 
CCCACCTGCC CCTCCCCTCC CCCTCTCCCC TCCACCACAG CAGCCCCTCT CCTCTCTCTG 
GCTAGTGCCT TCTCTCTGGC TGTGATGACT GTGGCCCAGT CCCTGCTGTC CCCATCCCCT 
GGGCTTCTCT CTCAGTCTCC TCCAGCCCCT CCAGGTCCTC TGCCTAGCAT GCCCCTTCCC 
CTTGCTTCTT GTGACCAGGA GAGCCTTTCA GCCCAAACAG CTGAGACAGA GAATGAGGCT 
TCCCGAAATC CTGCTCAGCC ACTACTGGGT GATGCTAGAC TGGCACCCAT ATCTGAAGAG 
GGAAAACCCC AGCTGGTTGG CCGATTCCAA GTGACTTCAT CCAAGGAACC AGCAGAGCCT 
CCCCTACAAC CAGCATCTCC AACTCTCTCC AGATCCCTAA AGCTTCCAAC CCCTCAGCTG 
ACCTCAGAGA GTTCAGACAC AGAGGACAGT GCTGCAGGAG GACCAGAGAC CAGGGAGGCT 
CTGGCAGAGA GTGACCGTGC AGCCGAGGGC TTGGGGGTCG CCATCGATGA GGAAAAGGAT 
GAAGGGAAGG AACCCCAAAT TGGGGGCAGT TCCCCAATCT TGAGCCAGCC CAGCCCAGTG 
TGGATGAACT ACTCCTACAG CAGCCTGTGT CTAAGCAGTG AGGAGTCAGA GAGCAGCGGG 
GAGGACGAGG AGTTCTGGGC TGAGCTGCAG AACCTTCGGC AGAAGCACTT GTCGGAAGTG 
GAAGCACTAC AGACGCTACA GAAGAAGGAA ATCGAAGACT TGTACAGCCG GCTTGGAAAG 
CAACCCCCAC CTGGTATTGT AGCTCCAGCG GCTATGCTGT CCTGCCGCCA GCGCCGCCTC 
TCCAAGGGAA GCTTTCCCAC CTCCCGCCGC AACAGCCTGC AGCGCTCTGA TCTCCCTGGC 
CCTGGCATCA TGCGAAGGAA CTCTCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGGTGAGGGG CGGGCCCAAG 
GGAGGGAGAG CTGAGGGAAT GGTGGCTGGC AGCAGCTCTG CCTTCCTCTT CTTCGGACAT 
TTCTTCAGCA CTTTTCTTTT CCCCTCCAGT GAATTCAGAC CAGAAGTCCT ATGTCTCCCC 
AACACCAGGG CCCCACCATG GCCCGTGGCC TCTCAGAATC TGATGTTTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247342.1
CDS	114..3407
Translation	MLIGSFFLPCLPLLCTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIQDLLTHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSMTPGPPSAFPPEPEEPEADQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGMPSSPAEPHLCLPSGFALSIPRSGPGSDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPSEATEDALSPQEEPAAMPALPGPSDAELQRSISPEQRSWAAFSTSPSSPGTPLSPGTPFSPGTPPVFPCPIFPITSPSCHPYPFSQVSSNPCPQAPSSLLPSSSGASQVPFPSPSLPTSSPLPFSPSYPQVPLHPASLPTCPSPPPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSMPLPLASCDQESLSAQTAETENEASRNPAQPLLGDARLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPTPQLTSESSDTEDSAAGGPETREALAESDRAAEGLGVAIDEEKDEGKEPQIGGSSPILSQPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRMVRGGPKGGRAEGMVAGSSSAFLFFGHFFSTFLFPSSEFRPEVLCLPNTRAPPWPVASQNLMFL

Target ORF information:

RefSeq Version	XM_006247280.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247280.3

ORF Insert Sequence:

ATGTTGATTG GCAGTTTCTT CCTCCCGTGT CTCCCACTGT TATGTACTCG GAAACTCTCT 
CGGGCTGAAC GGCAGCGTTT CTCTGAGGAA GTTGAGATGC TCAAGGGGCT GCAGCACCCC 
AACATCGTCC GCTTCTATGA CTCCTGGAAG TCGGTGCTGA GGGGTCAGGT TTGCATCGTG 
CTGGTCACAG AACTCATGAC CTCGGGCACG CTCAAGACGT ACCTGAGGAG ATTCCGAGAA 
ATGAAGCCCC GAGTCCTTCA GCGCTGGAGT CGCCAGATCC TACGGGGACT TCATTTCTTA 
CATTCCCGAG TGCCCCCCAT CCTGCACCGA GATCTCAAAT GTGACAATGT CTTCATTACC 
GGCCCTTCAG GCTCTGTCAA AATCGGAGAT CTCGGACTGG CCACGCTCAA GCGCGCCTCC 
TTTGCCAAGA GCGTCATTGG GACCCCGGAA TTCATGGCCC CTGAGATGTA TGAGGAAAAG 
TACGACGAGG CTGTGGATGT GTACGCCTTT GGCATGTGCA TGCTGGAGAT GGCTACGTCT 
GAGTATCCCT ACTCTGAGTG TCAGAACGCA GCACAAATCT ACCGAAAGGT CACTTCGGGC 
ACAAAGCCCA ACAGCTTCTA CAAAGTGAAG ATGCCAGAGG TGAAGGAGAT CATTGAAGGC 
TGCATCCGCA CGGATAAGAA TGAGAGGTTC ACTATCCAGG ATCTTCTGAC CCACGCGTTC 
TTCCGCGAGG AACGTGGTGT GCATGTGGAG CTGGCAGAGG AAGATGATGG AGAGAAGCCG 
GGTCTCAAAC TCTGGCTGCG CATGGAGGAC GCACGACGCG GGGGGCGCCC AAGGGACAAC 
CAGGCCATCG AGTTCCTGTT CCAGCTCGGT CGGGATGCAG CGGAAGAGGT GGCTCAGGAG 
ATGGTAGCCT TGGGCTTAGT CTGCGAAGCA GACTACCAGC CAGTGGCCCG TGCAGTTCGA 
GAACGGGTTG CTGCCATCCA GCGGAAGCGG GAGAAGCTAA GAAAAGCCAG GGAATTGGAG 
GTTCTCCCAC CAGACTCAGG ACCTCCTCCA GCAACTGTGT CTATGACTCC TGGTCCCCCC 
AGTGCCTTCC CCCCAGAGCC CGAGGAGCCA GAGGCTGACC AGCACCAATC CTTCCTCTTC 
CGCCATGCCA GCTACTCATC CACTACATCT GATTGCGAGA CTGATGGCTA CCTCAGCTCC 
TCCGGCTTCC TGGATGCCTC AGACCCTGCC CTTCAGCCCC CCGGGGGGAT GCCATCCAGC 
CCCGCTGAGC CCCATCTCTG CTTGCCCTCG GGTTTTGCCT TGTCCATTCC ACGGTCTGGC 
CCTGGCAGTG ACTTTTCTCC TGGGGACAGC TACGCCTCAG ATGCAGCCTC AGGCCTCAGT 
GACATGGGAG AAGGGGGGCA AATGAGGAAA AATCCAGTGA AGACTCTGCG ACGGAGACCT 
CGATCCCGGC TTCGGGTCAC GAGTGTCTCA GACCAGAGCG ACAGAGTCGT GGAGTGCCAG 
CTGCAAACTC ACAACAGCAA GATGGTGACG TTCCGATTTG ATCTGGATGG GGACAGCCCA 
GAAGAAATCG CAGCTGCCAT GGTTTATAAT GAATTCATTC TGCCCTCGGA GCGAGACGGA 
TTCCTGAGCC GGATCCGGGA GATTATCCAG CGAGTGGAGA CCCTGCTGAA GAGAGATGCT 
GGTCCCTCGG AGGCCACTGA AGATGCGCTG AGCCCTCAGG AAGAGCCAGC AGCCATGCCT 
GCCCTCCCAG GCCCATCCGA TGCAGAGCTC CAGAGAAGCA TCTCCCCGGA ACAGAGGAGC 
TGGGCAGCCT TCTCTACATC TCCATCTTCT CCTGGCACCC CCTTGTCCCC TGGAACCCCC 
TTTTCCCCTG GGACCCCTCC TGTCTTCCCA TGCCCCATCT TCCCTATCAC TTCACCCTCA 
TGCCATCCCT ACCCGTTCTC ACAGGTCTCT TCAAATCCCT GTCCACAGGC CCCCAGCTCC 
CTACTTCCCT CCTCCTCCGG TGCTTCTCAG GTTCCATTCC CATCCCCTTC CCTTCCCACC 
AGCTCTCCCC TCCCATTCTC TCCTAGTTAT CCCCAAGTCC CTCTTCACCC TGCTTCTCTT 
CCCACCTGCC CCTCCCCTCC CCCTCTCCCC TCCACCACAG CAGCCCCTCT CCTCTCTCTG 
GCTAGTGCCT TCTCTCTGGC TGTGATGACT GTGGCCCAGT CCCTGCTGTC CCCATCCCCT 
GGGCTTCTCT CTCAGTCTCC TCCAGCCCCT CCAGGTCCTC TGCCTAGCAT GCCCCTTCCC 
CTTGCTTCTT GTGACCAGGA GAGCCTTTCA GCCCAAACAG CTGAGACAGA GAATGAGGCT 
TCCCGAAATC CTGCTCAGCC ACTACTGGGT GATGCTAGAC TGGCACCCAT ATCTGAAGAG 
GGAAAACCCC AGCTGGTTGG CCGATTCCAA GTGACTTCAT CCAAGGAACC AGCAGAGCCT 
CCCCTACAAC CAGCATCTCC AACTCTCTCC AGATCCCTAA AGCTTCCAAC CCCTCAGCTG 
ACCTCAGAGA GTTCAGACAC AGAGGACAGT GCTGCAGGAG GACCAGAGAC CAGGGAGGCT 
CTGGCAGAGA GTGACCGTGC AGCCGAGGGC TTGGGGGTCG CCATCGATGA GGAAAAGGAT 
GAAGGGAAGG AACCCCAAAT TGGGGGCAGT TCCCCAATCT TGAGCCAGCC CAGCCCAGTG 
TGGATGAACT ACTCCTACAG CAGCCTGTGT CTAAGCAGTG AGGAGTCAGA GAGCAGCGGG 
GAGGACGAGG AGTTCTGGGC TGAGCTGCAG AACCTTCGGC AGAAGCACTT GTCGGAAGTG 
GAAGCACTAC AGACGCTACA GAAGAAGGAA ATCGAAGACT TGTACAGCCG GCTTGGAAAG 
CAACCCCCAC CTGGTATTGT AGCTCCAGCG GCTATGCTGT CCTGCCGCCA GCGCCGCCTC 
TCCAAGGGAA GCTTTCCCAC CTCCCGCCGC AACAGCCTGC AGCGCTCTGA TCTCCCTGGC 
CCTGGCATCA TGCGAAGGAA CTCTCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGGTGAGGGG CGGGCCCAAG 
GGAGGGAGAG CTGAGGGAAT GGTGGCTGGC AGCAGCTCTG CCTTCCTCTT CTTCGGACAT 
TTCTTCAGCA CTTTTCTTTT CCCCTCCAGT GAATTCAGAC CAGAAGTCCT ATGTCTCCCC 
AACACCAGGG CCCCACCATG GCCCGTGGCC TCTCAGAATC TGATGTTTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wnk4 ( XM_006247281.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247343.1 Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa33663
Clone ID Related Accession (Same CDS sequence)	XM_006247281.3
Accession Version	XM_006247281.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase WNK4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCC GAGTCCTTCA GCGCTGGAGT CGCCAGATCC TACGGGGACT TCATTTCTTA 
CATTCCCGAG TGCCCCCCAT CCTGCACCGA GATCTCAAAT GTGACAATGT CTTCATTACC 
GGCCCTTCAG GCTCTGTCAA AATCGGAGAT CTCGGACTGG CCACGCTCAA GCGCGCCTCC 
TTTGCCAAGA GCGTCATTGG GACCCCGGAA TTCATGGCCC CTGAGATGTA TGAGGAAAAG 
TACGACGAGG CTGTGGATGT GTACGCCTTT GGCATGTGCA TGCTGGAGAT GGCTACGTCT 
GAGTATCCCT ACTCTGAGTG TCAGAACGCA GCACAAATCT ACCGAAAGGT CACTTCGGGC 
ACAAAGCCCA ACAGCTTCTA CAAAGTGAAG ATGCCAGAGG TGAAGGAGAT CATTGAAGGC 
TGCATCCGCA CGGATAAGAA TGAGAGGTTC ACTATCCAGG ATCTTCTGAC CCACGCGTTC 
TTCCGCGAGG AACGTGGTGT GCATGTGGAG CTGGCAGAGG AAGATGATGG AGAGAAGCCG 
GGTCTCAAAC TCTGGCTGCG CATGGAGGAC GCACGACGCG GGGGGCGCCC AAGGGACAAC 
CAGGCCATCG AGTTCCTGTT CCAGCTCGGT CGGGATGCAG CGGAAGAGGT GGCTCAGGAG 
ATGGTAGCCT TGGGCTTAGT CTGCGAAGCA GACTACCAGC CAGTGGCCCG TGCAGTTCGA 
GAACGGGTTG CTGCCATCCA GCGGAAGCGG GAGAAGCTAA GAAAAGCCAG GGAATTGGAG 
GTTCTCCCAC CAGACTCAGG ACCTCCTCCA GCAACTGTGT CTATGACTCC TGGTCCCCCC 
AGTGCCTTCC CCCCAGAGCC CGAGGAGCCA GAGGCTGACC AGCACCAATC CTTCCTCTTC 
CGCCATGCCA GCTACTCATC CACTACATCT GATTGCGAGA CTGATGGCTA CCTCAGCTCC 
TCCGGCTTCC TGGATGCCTC AGACCCTGCC CTTCAGCCCC CCGGGGGGAT GCCATCCAGC 
CCCGCTGAGC CCCATCTCTG CTTGCCCTCG GGTTTTGCCT TGTCCATTCC ACGGTCTGGC 
CCTGGCAGTG ACTTTTCTCC TGGGGACAGC TACGCCTCAG ATGCAGCCTC AGGCCTCAGT 
GACATGGGAG AAGGGGGGCA AATGAGGAAA AATCCAGTGA AGACTCTGCG ACGGAGACCT 
CGATCCCGGC TTCGGGTCAC GAGTGTCTCA GACCAGAGCG ACAGAGTCGT GGAGTGCCAG 
CTGCAAACTC ACAACAGCAA GATGGTGACG TTCCGATTTG ATCTGGATGG GGACAGCCCA 
GAAGAAATCG CAGCTGCCAT GGTTTATAAT GAATTCATTC TGCCCTCGGA GCGAGACGGA 
TTCCTGAGCC GGATCCGGGA GATTATCCAG CGAGTGGAGA CCCTGCTGAA GAGAGATGCT 
GGTCCCTCGG AGGCCACTGA AGATGCGCTG AGCCCTCAGG AAGAGCCAGC AGCCATGCCT 
GCCCTCCCAG GCCCATCCGA TGCAGAGCTC CAGAGAAGCA TCTCCCCGGA ACAGAGGAGC 
TGGGCAGCCT TCTCTACATC TCCATCTTCT CCTGGCACCC CCTTGTCCCC TGGAACCCCC 
TTTTCCCCTG GGACCCCTCC TGTCTTCCCA TGCCCCATCT TCCCTATCAC TTCACCCTCA 
TGCCATCCCT ACCCGTTCTC ACAGGTCTCT TCAAATCCCT GTCCACAGGC CCCCAGCTCC 
CTACTTCCCT CCTCCTCCGG TGCTTCTCAG GTTCCATTCC CATCCCCTTC CCTTCCCACC 
AGCTCTCCCC TCCCATTCTC TCCTAGTTAT CCCCAAGTCC CTCTTCACCC TGCTTCTCTT 
CCCACCTGCC CCTCCCCTCC CCCTCTCCCC TCCACCACAG CAGCCCCTCT CCTCTCTCTG 
GCTAGTGCCT TCTCTCTGGC TGTGATGACT GTGGCCCAGT CCCTGCTGTC CCCATCCCCT 
GGGCTTCTCT CTCAGTCTCC TCCAGCCCCT CCAGGTCCTC TGCCTAGCAT GCCCCTTCCC 
CTTGCTTCTT GTGACCAGGA GAGCCTTTCA GCCCAAACAG CTGAGACAGA GAATGAGGCT 
TCCCGAAATC CTGCTCAGCC ACTACTGGGT GATGCTAGAC TGGCACCCAT ATCTGAAGAG 
GGAAAACCCC AGCTGGTTGG CCGATTCCAA GTGACTTCAT CCAAGGAACC AGCAGAGCCT 
CCCCTACAAC CAGCATCTCC AACTCTCTCC AGATCCCTAA AGCTTCCAAC CCCTCAGCTG 
ACCTCAGAGA GTTCAGACAC AGAGGACAGT GCTGCAGGAG GACCAGAGAC CAGGGAGGCT 
CTGGCAGAGA GTGACCGTGC AGCCGAGGGC TTGGGGGTCG CCATCGATGA GGAAAAGGAT 
GAAGGGAAGG AACCCCAAAT TGGGGGCAGT TCCCCAATCT TGAGCCAGCC CAGCCCAGTG 
TGGATGAACT ACTCCTACAG CAGCCTGTGT CTAAGCAGTG AGGAGTCAGA GAGCAGCGGG 
GAGGACGAGG AGTTCTGGGC TGAGCTGCAG AACCTTCGGC AGAAGCACTT GTCGGAAGTG 
GAAGCACTAC AGACGCTACA GAAGAAGGAA ATCGAAGACT TGTACAGCCG GCTTGGAAAG 
CAACCCCCAC CTGGTATTGT AGCTCCAGCG GCTATGCTGT CCTGCCGCCA GCGCCGCCTC 
TCCAAGGGAA GCTTTCCCAC CTCCCGCCGC AACAGCCTGC AGCGCTCTGA TCTCCCTGGC 
CCTGGCATCA TGCGAAGGAA CTCTCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGGTGAGGGG CGGGCCCAAG 
GGAGGGAGAG CTGAGGGAAT GGTGGCTGGC AGCAGCTCTG CCTTCCTCTT CTTCGGACAT 
TTCTTCAGCA CTTTTCTTTT CCCCTCCAGT GAATTCAGAC CAGAAGTCCT ATGTCTCCCC 
AACACCAGGG CCCCACCATG GCCCGTGGCC TCTCAGAATC TGATGTTTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247343.1
CDS	100..3153
Translation	MKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIQDLLTHAFFREERGVHVELAEEDDGEKPGLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEVLPPDSGPPPATVSMTPGPPSAFPPEPEEPEADQHQSFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGMPSSPAEPHLCLPSGFALSIPRSGPGSDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRVETLLKRDAGPSEATEDALSPQEEPAAMPALPGPSDAELQRSISPEQRSWAAFSTSPSSPGTPLSPGTPFSPGTPPVFPCPIFPITSPSCHPYPFSQVSSNPCPQAPSSLLPSSSGASQVPFPSPSLPTSSPLPFSPSYPQVPLHPASLPTCPSPPPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSMPLPLASCDQESLSAQTAETENEASRNPAQPLLGDARLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPTPQLTSESSDTEDSAAGGPETREALAESDRAAEGLGVAIDEEKDEGKEPQIGGSSPILSQPSPVWMNYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRLGKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRMVRGGPKGGRAEGMVAGSSSAFLFFGHFFSTFLFPSSEFRPEVLCLPNTRAPPWPVASQNLMFL

Target ORF information:

RefSeq Version	XM_006247281.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247281.3

ORF Insert Sequence:

ATGAAGCCCC GAGTCCTTCA GCGCTGGAGT CGCCAGATCC TACGGGGACT TCATTTCTTA 
CATTCCCGAG TGCCCCCCAT CCTGCACCGA GATCTCAAAT GTGACAATGT CTTCATTACC 
GGCCCTTCAG GCTCTGTCAA AATCGGAGAT CTCGGACTGG CCACGCTCAA GCGCGCCTCC 
TTTGCCAAGA GCGTCATTGG GACCCCGGAA TTCATGGCCC CTGAGATGTA TGAGGAAAAG 
TACGACGAGG CTGTGGATGT GTACGCCTTT GGCATGTGCA TGCTGGAGAT GGCTACGTCT 
GAGTATCCCT ACTCTGAGTG TCAGAACGCA GCACAAATCT ACCGAAAGGT CACTTCGGGC 
ACAAAGCCCA ACAGCTTCTA CAAAGTGAAG ATGCCAGAGG TGAAGGAGAT CATTGAAGGC 
TGCATCCGCA CGGATAAGAA TGAGAGGTTC ACTATCCAGG ATCTTCTGAC CCACGCGTTC 
TTCCGCGAGG AACGTGGTGT GCATGTGGAG CTGGCAGAGG AAGATGATGG AGAGAAGCCG 
GGTCTCAAAC TCTGGCTGCG CATGGAGGAC GCACGACGCG GGGGGCGCCC AAGGGACAAC 
CAGGCCATCG AGTTCCTGTT CCAGCTCGGT CGGGATGCAG CGGAAGAGGT GGCTCAGGAG 
ATGGTAGCCT TGGGCTTAGT CTGCGAAGCA GACTACCAGC CAGTGGCCCG TGCAGTTCGA 
GAACGGGTTG CTGCCATCCA GCGGAAGCGG GAGAAGCTAA GAAAAGCCAG GGAATTGGAG 
GTTCTCCCAC CAGACTCAGG ACCTCCTCCA GCAACTGTGT CTATGACTCC TGGTCCCCCC 
AGTGCCTTCC CCCCAGAGCC CGAGGAGCCA GAGGCTGACC AGCACCAATC CTTCCTCTTC 
CGCCATGCCA GCTACTCATC CACTACATCT GATTGCGAGA CTGATGGCTA CCTCAGCTCC 
TCCGGCTTCC TGGATGCCTC AGACCCTGCC CTTCAGCCCC CCGGGGGGAT GCCATCCAGC 
CCCGCTGAGC CCCATCTCTG CTTGCCCTCG GGTTTTGCCT TGTCCATTCC ACGGTCTGGC 
CCTGGCAGTG ACTTTTCTCC TGGGGACAGC TACGCCTCAG ATGCAGCCTC AGGCCTCAGT 
GACATGGGAG AAGGGGGGCA AATGAGGAAA AATCCAGTGA AGACTCTGCG ACGGAGACCT 
CGATCCCGGC TTCGGGTCAC GAGTGTCTCA GACCAGAGCG ACAGAGTCGT GGAGTGCCAG 
CTGCAAACTC ACAACAGCAA GATGGTGACG TTCCGATTTG ATCTGGATGG GGACAGCCCA 
GAAGAAATCG CAGCTGCCAT GGTTTATAAT GAATTCATTC TGCCCTCGGA GCGAGACGGA 
TTCCTGAGCC GGATCCGGGA GATTATCCAG CGAGTGGAGA CCCTGCTGAA GAGAGATGCT 
GGTCCCTCGG AGGCCACTGA AGATGCGCTG AGCCCTCAGG AAGAGCCAGC AGCCATGCCT 
GCCCTCCCAG GCCCATCCGA TGCAGAGCTC CAGAGAAGCA TCTCCCCGGA ACAGAGGAGC 
TGGGCAGCCT TCTCTACATC TCCATCTTCT CCTGGCACCC CCTTGTCCCC TGGAACCCCC 
TTTTCCCCTG GGACCCCTCC TGTCTTCCCA TGCCCCATCT TCCCTATCAC TTCACCCTCA 
TGCCATCCCT ACCCGTTCTC ACAGGTCTCT TCAAATCCCT GTCCACAGGC CCCCAGCTCC 
CTACTTCCCT CCTCCTCCGG TGCTTCTCAG GTTCCATTCC CATCCCCTTC CCTTCCCACC 
AGCTCTCCCC TCCCATTCTC TCCTAGTTAT CCCCAAGTCC CTCTTCACCC TGCTTCTCTT 
CCCACCTGCC CCTCCCCTCC CCCTCTCCCC TCCACCACAG CAGCCCCTCT CCTCTCTCTG 
GCTAGTGCCT TCTCTCTGGC TGTGATGACT GTGGCCCAGT CCCTGCTGTC CCCATCCCCT 
GGGCTTCTCT CTCAGTCTCC TCCAGCCCCT CCAGGTCCTC TGCCTAGCAT GCCCCTTCCC 
CTTGCTTCTT GTGACCAGGA GAGCCTTTCA GCCCAAACAG CTGAGACAGA GAATGAGGCT 
TCCCGAAATC CTGCTCAGCC ACTACTGGGT GATGCTAGAC TGGCACCCAT ATCTGAAGAG 
GGAAAACCCC AGCTGGTTGG CCGATTCCAA GTGACTTCAT CCAAGGAACC AGCAGAGCCT 
CCCCTACAAC CAGCATCTCC AACTCTCTCC AGATCCCTAA AGCTTCCAAC CCCTCAGCTG 
ACCTCAGAGA GTTCAGACAC AGAGGACAGT GCTGCAGGAG GACCAGAGAC CAGGGAGGCT 
CTGGCAGAGA GTGACCGTGC AGCCGAGGGC TTGGGGGTCG CCATCGATGA GGAAAAGGAT 
GAAGGGAAGG AACCCCAAAT TGGGGGCAGT TCCCCAATCT TGAGCCAGCC CAGCCCAGTG 
TGGATGAACT ACTCCTACAG CAGCCTGTGT CTAAGCAGTG AGGAGTCAGA GAGCAGCGGG 
GAGGACGAGG AGTTCTGGGC TGAGCTGCAG AACCTTCGGC AGAAGCACTT GTCGGAAGTG 
GAAGCACTAC AGACGCTACA GAAGAAGGAA ATCGAAGACT TGTACAGCCG GCTTGGAAAG 
CAACCCCCAC CTGGTATTGT AGCTCCAGCG GCTATGCTGT CCTGCCGCCA GCGCCGCCTC 
TCCAAGGGAA GCTTTCCCAC CTCCCGCCGC AACAGCCTGC AGCGCTCTGA TCTCCCTGGC 
CCTGGCATCA TGCGAAGGAA CTCTCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGGTGAGGGG CGGGCCCAAG 
GGAGGGAGAG CTGAGGGAAT GGTGGCTGGC AGCAGCTCTG CCTTCCTCTT CTTCGGACAT 
TTCTTCAGCA CTTTTCTTTT CCCCTCCAGT GAATTCAGAC CAGAAGTCCT ATGTCTCCCC 
AACACCAGGG CCCCACCATG GCCCGTGGCC TCTCAGAATC TGATGTTTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism37(8)2780-2794(2017 Aug)
Bhuiyan MIH,Song S,Yuan H,Begum G,Kofler J,Kahle KT,Yang SS,Lin SH,Alper SL,Subramanya AR,Sun D

Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and human lenses.
Investigative ophthalmology & visual science56(1)310-21(2014 Dec)
Vorontsova I,Lam L,Delpire E,Lim J,Donaldson P

A novel Ste20-related proline/alanine-rich kinase (SPAK)-independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in the activation of Na-K-Cl cotransporters.
The Journal of biological chemistry289(25)17680-8(2014 Jun)
Ponce-Coria J,Markadieu N,Austin TM,Flammang L,Rios K,Welling PA,Delpire E

Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via ubiquitination and degradation of WNK4.
Proceedings of the National Academy of Sciences of the United States of America110(19)7838-43(2013 May)
Shibata S,Zhang J,Puthumana J,Stone KL,Lifton RP

Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension.
Cell reports3(3)858-68(2013 Mar)
Wakabayashi M,Mori T,Isobe K,Sohara E,Susa K,Araki Y,Chiga M,Kikuchi E,Nomura N,Mori Y,Matsuo H,Murata T,Nomura S,Asano T,Kawaguchi H,Nonoyama S,Rai T,Sasaki S,Uchida S

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Wnk4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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