Spaca3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spaca3
Entrez Gene ID	287557
Full Name	sperm acrosome associated 3
Synonyms	Lyzc
General protein information	Preferred Names sperm acrosome associated 3 Names sperm acrosome membrane-associated protein 3 lysozyme c
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105820.1	NP_001099290.1	sperm acrosome membrane-associated protein 3 precursor
XM_006246983.3	XP_006247045.1	sperm acrosome membrane-associated protein 3 isoform X1

Pathways from BioSystems

Homologies

	LOC714491	XP_001113262.1
Bos taurus (cattle)	SPACA3	NP_001098477.1
Homo sapiens (human)	SPACA3	NP_776246.1
Mus musculus (house mouse)	Spaca3	NP_083643.1
Rattus norvegicus (Norway rat)	Spaca3	NP_001099290.1
Pan troglodytes (chimpanzee)	SPACA3	XP_001174564.1
Canis lupus familiaris (dog)	SPACA3	NP_001184016.1

Gene Ontology
Bibliography

Related articles in PubMed

Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization.
Herrero MB, Mandal A, Digilio LC, Coonrod SA, Maier B, Herr JC
Developmental biology284(1)126-42(2005 Aug)

SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa.
Mandal A, Klotz KL, Shetty J, Jayes FL, Wolkowicz MJ, Bolling LC, Coonrod SA, Black MB, Diekman AB, Haystead TA, Flickinger CJ, Herr JC
Biology of reproduction68(5)1525-37(2003 May)

Ultrastructural localization of lysozyme in human neutrophils by immunogold.
Cramer EM, Breton-Gorius J
Journal of leukocyte biology41(3)242-7(1987 Mar)

Distribution of lysosomal enzymes, cationic proteins, and bactericidal substances in subcellular fractions of human polymorphonuclear leukocytes.
Welsh IR, Spitznagel JK
Infection and immunity4(2)97-102(1971 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spaca3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spaca3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa33503	NM_001105820.1 Latest version!	Rattus norvegicus sperm acrosome associated 3 (Spaca3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa33503	XM_006246983.3 Latest version!	Rattus norvegicus sperm acrosome associated 3 (Spaca3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spaca3 ( NM_001105820.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099290.1 Rattus norvegicus sperm acrosome associated 3 (Spaca3), mRNA.

Price & Availability↑

CloneID	ORa33503
Clone ID Related Accession (Same CDS sequence)	NM_001105820.1 , XM_006246983.3
Accession Version	NM_001105820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	sperm acrosome membrane-associated protein 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 3, 2007 this sequence version replaced XM_213416.3, XM_001080990.1.

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCCGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGTGCT GCATGACTTC GGTCTGGAAG GCTACAGGGG ATATAACCTG 
GCTGACTGGA TCTGCCTTGC GTACTACACA AGCGGCTTCA ATACGGATGC TGTGGATCAC 
GAAGCTGACG GAAGCACCAA CAACGGCATC TTCCAGATCA GCAGCCGGAA GTGGTGCAAA 
AACCTCGCCC CGAACGGCCC CAATCTTTGC AGGATATACT GCACGGATTT GTTGAGCAAT 
GATCTCAAAG ATTCTGTCGC CTGTGTCATG AAGATAGCGC AAGAGCCCCA GGGCCTGGGC 
TACTGGGAAT CCTGGAAGCA CCATTGTCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099290.1
CDS	333..824
Translation	MEARSRAPRRQPCPPGITWLALAYLLSCLLASSKAKVFSRCELAKVLHDFGLEGYRGYNLADWICLAYYTSGFNTDAVDHEADGSTNNGIFQISSRKWCKNLAPNGPNLCRIYCTDLLSNDLKDSVACVMKIAQEPQGLGYWESWKHHCQGRDLSDWVDGCDF

Target ORF information:

RefSeq Version	NM_001105820.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm acrosome associated 3 (Spaca3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105820.1

ORF Insert Sequence:

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCCGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGTGCT GCATGACTTC GGTCTGGAAG GCTACAGGGG ATATAACCTG 
GCTGACTGGA TCTGCCTTGC GTACTACACA AGCGGCTTCA ATACGGATGC TGTGGATCAC 
GAAGCTGACG GAAGCACCAA CAACGGCATC TTCCAGATCA GCAGCCGGAA GTGGTGCAAA 
AACCTCGCCC CGAACGGCCC CAATCTTTGC AGGATATACT GCACGGATTT GTTGAGCAAT 
GATCTCAAAG ATTCTGTCGC CTGTGTCATG AAGATAGCGC AAGAGCCCCA GGGCCTGGGC 
TACTGGGAAT CCTGGAAGCA CCATTGTCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spaca3 ( XM_006246983.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247045.1 Rattus norvegicus sperm acrosome associated 3 (Spaca3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33503
Clone ID Related Accession (Same CDS sequence)	NM_001105820.1 , XM_006246983.3
Accession Version	XM_006246983.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sperm acrosome membrane-associated protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCCGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGTGCT GCATGACTTC GGTCTGGAAG GCTACAGGGG ATATAACCTG 
GCTGACTGGA TCTGCCTTGC GTACTACACA AGCGGCTTCA ATACGGATGC TGTGGATCAC 
GAAGCTGACG GAAGCACCAA CAACGGCATC TTCCAGATCA GCAGCCGGAA GTGGTGCAAA 
AACCTCGCCC CGAACGGCCC CAATCTTTGC AGGATATACT GCACGGATTT GTTGAGCAAT 
GATCTCAAAG ATTCTGTCGC CTGTGTCATG AAGATAGCGC AAGAGCCCCA GGGCCTGGGC 
TACTGGGAAT CCTGGAAGCA CCATTGTCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247045.1
CDS	229..720
Translation	MEARSRAPRRQPCPPGITWLALAYLLSCLLASSKAKVFSRCELAKVLHDFGLEGYRGYNLADWICLAYYTSGFNTDAVDHEADGSTNNGIFQISSRKWCKNLAPNGPNLCRIYCTDLLSNDLKDSVACVMKIAQEPQGLGYWESWKHHCQGRDLSDWVDGCDF

Target ORF information:

RefSeq Version	XM_006246983.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm acrosome associated 3 (Spaca3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246983.3

ORF Insert Sequence:

ATGGAAGCTA GGAGCCGGGC TCCCAGAAGA CAGCCGTGCC CGCCTGGGAT CACTTGGCTG 
GCCCTGGCCT ATCTGCTCAG CTGCCTGCTT GCCTCCAGCA AGGCCAAGGT CTTCAGTCGC 
TGTGAGCTGG CCAAAGTGCT GCATGACTTC GGTCTGGAAG GCTACAGGGG ATATAACCTG 
GCTGACTGGA TCTGCCTTGC GTACTACACA AGCGGCTTCA ATACGGATGC TGTGGATCAC 
GAAGCTGACG GAAGCACCAA CAACGGCATC TTCCAGATCA GCAGCCGGAA GTGGTGCAAA 
AACCTCGCCC CGAACGGCCC CAATCTTTGC AGGATATACT GCACGGATTT GTTGAGCAAT 
GATCTCAAAG ATTCTGTCGC CTGTGTCATG AAGATAGCGC AAGAGCCCCA GGGCCTGGGC 
TACTGGGAAT CCTGGAAGCA CCATTGTCAG GGCAGGGACC TCAGTGACTG GGTGGATGGC 
TGTGACTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg binding and fertilization.
Developmental biology284(1)126-42(2005 Aug)
Herrero MB,Mandal A,Digilio LC,Coonrod SA,Maier B,Herr JC

SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa.
Biology of reproduction68(5)1525-37(2003 May)
Mandal A,Klotz KL,Shetty J,Jayes FL,Wolkowicz MJ,Bolling LC,Coonrod SA,Black MB,Diekman AB,Haystead TA,Flickinger CJ,Herr JC

Ultrastructural localization of lysozyme in human neutrophils by immunogold.
Journal of leukocyte biology41(3)242-7(1987 Mar)
Cramer EM,Breton-Gorius J

Distribution of lysosomal enzymes, cationic proteins, and bactericidal substances in subcellular fractions of human polymorphonuclear leukocytes.
Infection and immunity4(2)97-102(1971 Aug)
Welsh IR,Spitznagel JK