gRNAs
The following Smg6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa44589 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008767960.2 | |
| Accession Version | XM_008767960.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | telomerase-binding protein EST1A isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTTGGCT ACCCAGACAC CTGGAATCCT CCGCCCACAT CTTTGGATCT GCCCTTGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008766182.1 |
| CDS | 1832..2995 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_008767960.2 |
1 | ATGCTTGGCT ACCCAGACAC CTGGAATCCT CCGCCCACAT CTTTGGATCT GCCCTTGCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa33422 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006246866.3 | |
| Accession Version | XM_006246866.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | telomerase-binding protein EST1A isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTTTTGC TCCCAGTGCG GCCACCGTCG TTTGGGGAGG GCAGGCAGGA GGGGGCGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006246928.1 |
| CDS | 1279..4110 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006246866.3 |
1 | ATGCTTTTGC TCCCAGTGCG GCCACCGTCG TTTGGGGAGG GCAGGCAGGA GGGGGCGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa11455 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001105808.1 | |
| Accession Version | NM_001105808.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-27 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | telomerase-binding protein EST1A | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473948.1. On or before Oct 3, 2007 this sequence version replaced XM_001080444.1, XM_220702.4. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCTTTTGC TCCCAGTGCG GCCACCGTCG TTTGGGGAGG GCAGGCAGGA GGGGGCGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001099278.1 |
| CDS | 420..4838 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001105808.1 |
1 | ATGCTTTTGC TCCCAGTGCG GCCACCGTCG TTTGGGGAGG GCAGGCAGGA GGGGGCGGGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The TPR-containing domain within Est1 homologs exhibits species-specific roles in telomerase interaction and telomere length homeostasis. |
SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay. |
SMG6 promotes endonucleolytic cleavage of nonsense mRNA in human cells. |
SMG6 is the catalytic endonuclease that cleaves mRNAs containing nonsense codons in metazoan. |
Protein RNA and protein protein interactions mediate association of human EST1A/SMG6 with telomerase. |
