Eif5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif5a
Entrez Gene ID	287444
Full Name	eukaryotic translation initiation factor 5A
General protein information	Preferred Names eukaryotic translation initiation factor 5A Names eukaryotic translation initiation factor 5A-1 eIF-4D eIF-5A eIF-5A-1 eIF-5A1 eukaryotic initiation factor 5A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246637.3	XP_006246699.1	eukaryotic translation initiation factor 5A-1 isoform X1
NM_001033681.1	NP_001028853.1	eukaryotic translation initiation factor 5A-1
XM_006246638.1	XP_006246700.1	eukaryotic translation initiation factor 5A-1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	EIF5A	NP_001003658.1
Pan troglodytes (chimpanzee)	EIF5A	XP_003315395.1
Mus musculus (house mouse)	Eif5a	NP_001160068.1
Xenopus tropicalis (tropical clawed frog)	eif5a	NP_001004855.1
Canis lupus familiaris (dog)	EIF5A	XP_005620026.1
Rattus norvegicus (Norway rat)	Eif5a	NP_001028853.1
Homo sapiens (human)	EIF5A	NP_001137232.1
	LOC721812	XP_001118011.2
Danio rerio (zebrafish)	eif5a2	NP_998427.1

Gene Ontology
Bibliography

Related articles in PubMed

LHCGR Expression During Follicle Stimulating Hormone-Induced Follicle Growth Is Negatively Regulated by Eukaryotic Initiation Factor 5A.
Gulappa T, Menon B, Menon KMJ
Endocrinology158(8)2672-2679(2017 Aug)

miR-434-3p and DNA hypomethylation co-regulate eIF5A1 to increase AChRs and to improve plasticity in SCT rat skeletal muscle.
Shang FF, Xia QJ, Liu W, Xia L, Qian BJ, You L, He M, Yang JL, Wang TH
Scientific reports622884(2016 Mar)

Plasma levels of oxidative stress-responsive apoptosis inducing protein (ORAIP) in rats subjected to physicochemical oxidative stresses.
Yao T, Fujimura T, Murayama K, Seko Y
Bioscience reports36(2)(2016)

eIF5A1/RhoGDI? pathway: a novel therapeutic target for treatment of spinal cord injury identified by a proteomics approach.
Liu W, Shang FF, Xu Y, Belegu V, Xia L, Zhao W, Liu R, Wang W, Liu J, Li CY, Wang TH
Scientific reports516911(2015 Nov)

Hypusination of eukaryotic initiation factor 5A via cAMP-PKA-ERK1/2 pathway is required for ligand-induced downregulation of LH receptor mRNA expression in the ovary.
Gulappa T, Menon B, Menon KM
Molecular and cellular endocrinology41390-5(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study support the notion that by inhibiting eIF5A hypusination, FSH reduces the expression of an LHCGR mRNA-binding protein (LRBP), and then this increases LHCGR mRNA expression by abrogating the inhibitory action of LRBP.
Title: LHCGR Expression During Follicle Stimulating Hormone-Induced Follicle Growth Is Negatively Regulated by Eukaryotic Initiation Factor 5A.

Overexpression or knockdown of miR-434-3p in vivo demonstrated it was a negative post-transcriptional regulator of eIF5A1 expression and influenced AChRs expression.
Title: miR-434-3p and DNA hypomethylation co-regulate eIF5A1 to increase AChRs and to improve plasticity in SCT rat skeletal muscle.

secretion of ORAIP in response to oxidative stress is universal mechanism and plays an essential role. ORAIP will be an important novel biomarker as well as a specific therapeutic target of these oxidative stress-induced cell injuries.
Title: Plasma levels of oxidative stress-responsive apoptosis inducing protein (ORAIP) in rats subjected to physicochemical oxidative stresses.

eIF5A1 upregulates RhoGDIalpha to promote neuroplasticity caudal to the spinal cord injury and functional recovery after spinal cord injury.
Title: eIF5A1/RhoGDI? pathway: a novel therapeutic target for treatment of spinal cord injury identified by a proteomics approach.

these results suggest that hCG-induced LHR mRNA downregulation is mediated by cAMP-PKA-ERK1/2 signaling leading to activation of eIF5A hypusination.
Title: Hypusination of eukaryotic initiation factor 5A via cAMP-PKA-ERK1/2 pathway is required for ligand-induced downregulation of LH receptor mRNA expression in the ovary.

The following Eif5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08550	NM_001033681.1 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa08550	XM_006246638.1 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa08550	XM_006246637.3 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif5a ( NM_001033681.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001028853.1 Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), mRNA.

Price & Availability↑

CloneID	ORa08550
Clone ID Related Accession (Same CDS sequence)	XM_006246637.3 , NM_001033681.1 , XM_006246638.1
Accession Version	NM_001033681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic translation initiation factor 5A-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC101891.1. On Sep 25, 2005 this sequence version replaced XM_213368.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC101891.1, FQ231501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGATG ATTTGGACTT CGAGACAGGA GATGCAGGGG CCTCAGCCAC CTTCCCAATG 
CAGTGCTCAG CATTACGTAA GAATGGTTTT GTGGTGCTCA AGGGCCGGCC ATGTAAGATC 
GTCGAGATGT CTACTTCGAA GACTGGCAAG CATGGCCATG CCAAGGTCCA TCTGGTTGGT 
ATTGATATTT TTACTGGGAA GAAATATGAA GATATCTGCC CGTCGACTCA TAACATGGAT 
GTCCCCAACA TCAAAAGGAA TGATTTCCAG CTGATTGGCA TCCAGGATGG GTACCTATCC 
CTGCTCCAGG ACAGTGGGGA GGTACGAGAG GACCTTCGTC TGCCTGAGGG AGACCTTGGC 
AAGGAGATTG AGCAGAAGTA TGACTGTGGA GAAGAGATCC TGATCACAGT GCTGTCCGCC 
ATGACAGAGG AGGCAGCTGT TGCAATCAAG GCCATGGCAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001028853.1
CDS	113..577
Translation	MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

Target ORF information:

RefSeq Version	NM_001033681.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033681.1

ORF Insert Sequence:

ATGGCAGATG ATTTGGACTT CGAGACAGGA GATGCAGGGG CCTCAGCCAC CTTCCCAATG 
CAGTGCTCAG CATTACGTAA GAATGGTTTT GTGGTGCTCA AGGGCCGGCC ATGTAAGATC 
GTCGAGATGT CTACTTCGAA GACTGGCAAG CATGGCCATG CCAAGGTCCA TCTGGTTGGT 
ATTGATATTT TTACTGGGAA GAAATATGAA GATATCTGCC CGTCGACTCA TAACATGGAT 
GTCCCCAACA TCAAAAGGAA TGATTTCCAG CTGATTGGCA TCCAGGATGG GTACCTATCC 
CTGCTCCAGG ACAGTGGGGA GGTACGAGAG GACCTTCGTC TGCCTGAGGG AGACCTTGGC 
AAGGAGATTG AGCAGAAGTA TGACTGTGGA GAAGAGATCC TGATCACAGT GCTGTCCGCC 
ATGACAGAGG AGGCAGCTGT TGCAATCAAG GCCATGGCAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif5a ( XM_006246637.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246699.1 Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa08550
Clone ID Related Accession (Same CDS sequence)	XM_006246637.3 , NM_001033681.1 , XM_006246638.1
Accession Version	XM_006246637.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic translation initiation factor 5A-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATTTGGACTT CGAGACAGGA GATGCAGGGG CCTCAGCCAC CTTCCCAATG 
CAGTGCTCAG CATTACGTAA GAATGGTTTT GTGGTGCTCA AGGGCCGGCC ATGTAAGATC 
GTCGAGATGT CTACTTCGAA GACTGGCAAG CATGGCCATG CCAAGGTCCA TCTGGTTGGT 
ATTGATATTT TTACTGGGAA GAAATATGAA GATATCTGCC CGTCGACTCA TAACATGGAT 
GTCCCCAACA TCAAAAGGAA TGATTTCCAG CTGATTGGCA TCCAGGATGG GTACCTATCC 
CTGCTCCAGG ACAGTGGGGA GGTACGAGAG GACCTTCGTC TGCCTGAGGG AGACCTTGGC 
AAGGAGATTG AGCAGAAGTA TGACTGTGGA GAAGAGATCC TGATCACAGT GCTGTCCGCC 
ATGACAGAGG AGGCAGCTGT TGCAATCAAG GCCATGGCAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246699.1
CDS	339..803
Translation	MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

Target ORF information:

RefSeq Version	XM_006246637.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246637.3

ORF Insert Sequence:

ATGGCAGATG ATTTGGACTT CGAGACAGGA GATGCAGGGG CCTCAGCCAC CTTCCCAATG 
CAGTGCTCAG CATTACGTAA GAATGGTTTT GTGGTGCTCA AGGGCCGGCC ATGTAAGATC 
GTCGAGATGT CTACTTCGAA GACTGGCAAG CATGGCCATG CCAAGGTCCA TCTGGTTGGT 
ATTGATATTT TTACTGGGAA GAAATATGAA GATATCTGCC CGTCGACTCA TAACATGGAT 
GTCCCCAACA TCAAAAGGAA TGATTTCCAG CTGATTGGCA TCCAGGATGG GTACCTATCC 
CTGCTCCAGG ACAGTGGGGA GGTACGAGAG GACCTTCGTC TGCCTGAGGG AGACCTTGGC 
AAGGAGATTG AGCAGAAGTA TGACTGTGGA GAAGAGATCC TGATCACAGT GCTGTCCGCC 
ATGACAGAGG AGGCAGCTGT TGCAATCAAG GCCATGGCAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eif5a ( XM_006246638.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246700.1 Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa08550
Clone ID Related Accession (Same CDS sequence)	XM_006246637.3 , NM_001033681.1 , XM_006246638.1
Accession Version	XM_006246638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic translation initiation factor 5A-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATTTGGACTT CGAGACAGGA GATGCAGGGG CCTCAGCCAC CTTCCCAATG 
CAGTGCTCAG CATTACGTAA GAATGGTTTT GTGGTGCTCA AGGGCCGGCC ATGTAAGATC 
GTCGAGATGT CTACTTCGAA GACTGGCAAG CATGGCCATG CCAAGGTCCA TCTGGTTGGT 
ATTGATATTT TTACTGGGAA GAAATATGAA GATATCTGCC CGTCGACTCA TAACATGGAT 
GTCCCCAACA TCAAAAGGAA TGATTTCCAG CTGATTGGCA TCCAGGATGG GTACCTATCC 
CTGCTCCAGG ACAGTGGGGA GGTACGAGAG GACCTTCGTC TGCCTGAGGG AGACCTTGGC 
AAGGAGATTG AGCAGAAGTA TGACTGTGGA GAAGAGATCC TGATCACAGT GCTGTCCGCC 
ATGACAGAGG AGGCAGCTGT TGCAATCAAG GCCATGGCAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246700.1
CDS	332..796
Translation	MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

Target ORF information:

RefSeq Version	XM_006246638.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 5A (Eif5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246638.1

ORF Insert Sequence:

ATGGCAGATG ATTTGGACTT CGAGACAGGA GATGCAGGGG CCTCAGCCAC CTTCCCAATG 
CAGTGCTCAG CATTACGTAA GAATGGTTTT GTGGTGCTCA AGGGCCGGCC ATGTAAGATC 
GTCGAGATGT CTACTTCGAA GACTGGCAAG CATGGCCATG CCAAGGTCCA TCTGGTTGGT 
ATTGATATTT TTACTGGGAA GAAATATGAA GATATCTGCC CGTCGACTCA TAACATGGAT 
GTCCCCAACA TCAAAAGGAA TGATTTCCAG CTGATTGGCA TCCAGGATGG GTACCTATCC 
CTGCTCCAGG ACAGTGGGGA GGTACGAGAG GACCTTCGTC TGCCTGAGGG AGACCTTGGC 
AAGGAGATTG AGCAGAAGTA TGACTGTGGA GAAGAGATCC TGATCACAGT GCTGTCCGCC 
ATGACAGAGG AGGCAGCTGT TGCAATCAAG GCCATGGCAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LHCGR Expression During Follicle Stimulating Hormone-Induced Follicle Growth Is Negatively Regulated by Eukaryotic Initiation Factor 5A.
Endocrinology158(8)2672-2679(2017 Aug)
Gulappa T,Menon B,Menon KMJ

miR-434-3p and DNA hypomethylation co-regulate eIF5A1 to increase AChRs and to improve plasticity in SCT rat skeletal muscle.
Scientific reports622884(2016 Mar)
Shang FF,Xia QJ,Liu W,Xia L,Qian BJ,You L,He M,Yang JL,Wang TH

Plasma levels of oxidative stress-responsive apoptosis inducing protein (ORAIP) in rats subjected to physicochemical oxidative stresses.
Bioscience reports36(2)(2016)
Yao T,Fujimura T,Murayama K,Seko Y

eIF5A1/RhoGDI? pathway: a novel therapeutic target for treatment of spinal cord injury identified by a proteomics approach.
Scientific reports516911(2015 Nov)
Liu W,Shang FF,Xu Y,Belegu V,Xia L,Zhao W,Liu R,Wang W,Liu J,Li CY,Wang TH

Hypusination of eukaryotic initiation factor 5A via cAMP-PKA-ERK1/2 pathway is required for ligand-induced downregulation of LH receptor mRNA expression in the ovary.
Molecular and cellular endocrinology41390-5(2015 Sep)
Gulappa T,Menon B,Menon KM

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Eif5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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