Lgr4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lgr4
Entrez Gene ID	286994
Full Name	leucine-rich repeat-containing G protein-coupled receptor 4
Synonyms	Gpr48
General protein information	Preferred Names leucine-rich repeat-containing G protein-coupled receptor 4 Names leucine-rich repeat-containing G-protein coupled receptor 4 G-protein coupled receptor 48
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q34
Summary	member of a family of G-protein coupled receptors that contain leucine rich repeats [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_173328.1	NP_775450.1	leucine-rich repeat-containing G-protein coupled receptor 4 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LGR4	XP_005633820.1
Pan troglodytes (chimpanzee)	LGR4	XP_003313024.2
Homo sapiens (human)	LGR4	NP_060960.2
Rattus norvegicus (Norway rat)	Lgr4	NP_775450.1
	LGR4	XP_001090038.2
Bos taurus (cattle)	LGR4	NP_001192440.1
Mus musculus (house mouse)	Lgr4	NP_766259.2
Gallus gallus (chicken)	LGR4	XP_426162.3
Xenopus tropicalis (tropical clawed frog)	lgr4	NP_001119970.1
Danio rerio (zebrafish)	lgr4	XP_687184.4

Gene Ontology
Bibliography

Related articles in PubMed

Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin development in mice.
Kinzel B, Pikiolek M, Orsini V, Sprunger J, Isken A, Zietzling S, Desplanches M, Dubost V, Breustedt D, Valdez R, Liu D, Theil D, M?ller M, Dietrich B, Bouwmeester T, Ruffner H, Tchorz JS
Developmental biology390(2)181-90(2014 Jun)

LGR4 acts as a link between the peripheral circadian clock and lipid metabolism in liver.
Wang F, Zhang X, Wang J, Chen M, Fan N, Ma Q, Liu R, Wang R, Li X, Liu M, Ning G
Journal of molecular endocrinology52(2)133-43(2014 Apr)

LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats.
Li JY, Chai B, Zhang W, Fritze DM, Zhang C, Mulholland MW
Endocrinology155(2)429-40(2014 Feb)

A naturally occurring Lgr4 splice variant encodes a soluble antagonist useful for demonstrating the gonadal roles of Lgr4 in mammals.
Hsu PJ, Wu FJ, Kudo M, Hsiao CL, Hsueh AJ, Luo CW
PloS one9(9)e106804(2014)

Ablation of LGR4 promotes energy expenditure by driving white-to-brown fat switch.
Wang J, Liu R, Wang F, Hong J, Li X, Chen M, Ke Y, Zhang X, Ma Q, Wang R, Shi J, Cui B, Gu W, Zhang Y, Zhang Z, Wang W, Xia X, Liu M, Ning G
Nature cell biology15(12)1455-63(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data indicate that Rspo1 and Rspo3 and their receptor LGR4 form novel circuits in the brain to regulate energy homeostasis.
Title: LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats.

The endogenously expressed Lgr4 may act as an antagonist molecule that helps to fine-tune the R-spondin/norrin-mediated Lgr4-Wnt signaling during gonadal development.
Title: A naturally occurring Lgr4 splice variant encodes a soluble antagonist useful for demonstrating the gonadal roles of Lgr4 in mammals.

The following Lgr4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lgr4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12564	NM_173328.1 Latest version!	Rattus norvegicus leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lgr4 ( NM_173328.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775450.1 Rattus norvegicus leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), mRNA.

Price & Availability↑

CloneID	ORa12564
Clone ID Related Accession (Same CDS sequence)	NM_173328.1
Accession Version	NM_173328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF061443.1. Sequence Note: This sequence has been modified as follows: removed 24 bp suspected to be vector contamination from the 3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF061443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGAAAGGG GTTGACGGCC GTACCGGAGG GTCTCAGCGC CTTCACCCAA 
GCACTGGATA TCAGTATGAA CAATATCACC CAGTTACCAG AAGATGCATT TAAGAGTTTC 
CCATTTCTAG AGGAGCTACA ACTGGCTGGT AACGACCTTT CTCTTATCCA TCCAAAAGCC 
TTGTCTGGGC TGAAAGAACT CAAAGTCCTA ACACTCCAGA ATAATCAGTT GAGAACAGTG 
CCCAGTGAAG CCATTCACGG ACTGAGTGCT TTGCAGTCTT TACGCTTAGA TGCCAACCAT 
ATTACCTCAG TCCCGGAGGA CAGTTTTGAA GGGCTTGTCC AGTTACGCCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA AGTGCCCGTG CGTCCCCTCA GCAACCTGCC AACCCTGCAG 
GCGCTGACCT TGGCTCTCAA CAACATCTCA AGCATCCCTG ACTTCGCTTT CACCAACCTT 
TCAAGCTTGG TGGTTCTGCA TCTGCATAAC AATAAAATTA AAAGCCTCAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAAACCTTG GACTTGAATT ACAATTACTT GGATGAGTTT 
CCTCAGGCTA TTAAAGCCCT TCCCAGCCTT AAAGAGCTGG GATTTCACAG TAATTCTATT 
TCTGTTATTC CTGATGGAGC ATTTGGTGGT AATCCACTGC TAAGAACTAT TCATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ACCTGTCTGA TCTGCATTGC 
TTAGTCATTC GTGGTGCAAG CCTGGTGCAG TGGTTCCCCA ATCTGACCGG AACTGTCCAT 
TTGGAGAGTC TAACCTTGAC AGGGACAAAA ATAAGCAGCA TACCTGATGA TCTGTGCCAA 
AACCAAAAGA TGCTGAGGAC TCTGGACTTA TCTTATAACA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GTCGTGCATT GGAAGAAATT TCATTGCAGC GTAATCAAAT CTCCCTAATA 
AAGGAAAATA CTTTTCAAGG CCTAACATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATCCGTGAAA TTCACAGTGG AGCTTTTGCG AAGCTTGGGA CAATTACTAA CCTGGATGTA 
AGTTTCAATG AATTAACTTC ATTTCCTACG GAAGGCCTAA ATGGGCTCAA TCAACTAAAG 
CTTGTGGGTA ACTTCAAGCT GAAAGACGCC TTGGCAGCCA GAGACTTTGC TAATCTCAGG 
TCTCTATCAG TACCATATGC TTATCAGTGT TGTGCATTTT GGGGGTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCCCCAA GAACACAGTG TGACAAAAGA GAAAGGTGCT 
ACAGATGCAG CAAATGTCAC CAGCACTGCT GAGAACGAAG AACATAGCCA AATAATTATC 
CACTGTACAC CTTCAACAGG TGCTTTCAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGCCTTA CAGTGTGGTT CATTTTCCTG GTCGCCTTGC TTTTCAACCT GCTTGTCATT 
TTAACAGTGT TTGCGTCTTG TTCATCACTG CCTGCCTCCA AACTCTTCAT AGGCTTGATT 
TCTGTGTCTA ACTTACTCAT GGGCATCTAT ACTGGCATCC TTACTTTTCT TGATGCTGTG 
TCCTGGGGCC GATTTGCCGA ATTTGGCATT TGGTGGGAAA CTGGCAGCGG CTGCAAGGTA 
GCCGGGTCTC TGGCAGTCTT CTCCTCAGAG AGCGCTGTAT TCCTATTAAC ACTGGCAGCT 
GTGGAAAGAA GCGTATTTGC AAAGGATTTG ATGAAACACG GGAAGAGCAG TCACCTCAGA 
CAGTTCCAGG TGGCCGCCCT CTTAGCTTTG CTGGGTGCCG CAGTGGCAGG CTGCTTCCCC 
CTTTTCCACG GAGGGCAATA TTCTGCATCG CCCTTGTGTT TGCCGTTTCC TACAGGAGAA 
ACCCCATCGT TAGGATTCAC TGTGACCTTA GTGCTATTAA ACTCACTGGC ATTTTTACTA 
ATGGCCATTA TCTACACTAA ACTATACTGC AACTTAGAGA AGGAGGACCT GTCGGAAAAC 
TCCCAGTCTA GCGTGATTAA GCACGTTGCC TGGCTCATCT TCACAAACTG CATCTTCTTC 
TGCCCTGTTG CATTTTTCTC ATTTGCACCA TTGATCACGG CAATCTCCAT CAGCCCCGAG 
ATAATGAAGT CTGTTACACT GATATTCTTC CCGTTGCCTG CTTGCCTGAA TCCGGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGC TACTGAAGCG GCGTGTTACC 
AGGAAACACG GATCTGTTTC AGTTTCCATC AGCAGCCAAG GCGGTTGTGG GGAACAGGAT 
TTCTACTATG ACTGTGGCAT GTATTCCCAC TTGCAGGGTA ACCTGACTGT CTGTGACTGC 
TGTGAGTCAT TTCTTTTGAC AAAACCAGTA TCATGCAAAC ACTTAATAAA ATCGCACAGT 
TGTCCTGTAT TGACAGCGGC CTCTTGCCAG AGGCCAGAGG CCTACTGGTC TGATTGTGGT 
ACACAGTCAG CCCATTCTGA CTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGCTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTCGTGGATT CCCTCTGGTG 
CGCTATGCTT ACAATCTACA GAGAGTCAGA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775450.1
CDS	44..2899
Translation	MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNITQLPEDAFKSFPFLEELQLAGNDLSLIHPKALSGLKELKVLTLQNNQLRTVPSEAIHGLSALQSLRLDANHITSVPEDSFEGLVQLRHLWLDDNSLTEVPVRPLSNLPTLQALTLALNNISSIPDFAFTNLSSLVVLHLHNNKIKSLSQHCFDGLDNLETLDLNYNYLDEFPQAIKALPSLKELGFHSNSISVIPDGAFGGNPLLRTIHLYDNPLSFVGNSAFHNLSDLHCLVIRGASLVQWFPNLTGTVHLESLTLTGTKISSIPDDLCQNQKMLRTLDLSYNNIRDLPSFNGCRALEEISLQRNQISLIKENTFQGLTSLRILDLSRNLIREIHSGAFAKLGTITNLDVSFNELTSFPTEGLNGLNQLKLVGNFKLKDALAARDFANLRSLSVPYAYQCCAFWGCDSYANLNTEDNSPQEHSVTKEKGATDAANVTSTAENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALLFNLLVILTVFASCSSLPASKLFIGLISVSNLLMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGSLAVFSSESAVFLLTLAAVERSVFAKDLMKHGKSSHLRQFQVAALLALLGAAVAGCFPLFHGGQYSASPLCLPFPTGETPSLGFTVTLVLLNSLAFLLMAIIYTKLYCNLEKEDLSENSQSSVIKHVAWLIFTNCIFFCPVAFFSFAPLITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTRKHGSVSVSISSQGGCGEQDFYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPVLTAASCQRPEAYWSDCGTQSAHSDYADEEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLQRVRD

Target ORF information:

RefSeq Version	NM_173328.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine-rich repeat-containing G protein-coupled receptor 4 (Lgr4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173328.1

ORF Insert Sequence:

ATGCCGGGCC CGCTAGGGCT GCTCTGCTTC CTCGCCCTGG GGCTGCTCGG CTCGGCCGGG 
CCCAGCGGCG CGGCGCCGCC TCTCTGCGCG GCGCCCTGCA GCTGCGACGG CGACCGTCGG 
GTGGACTGCT CCGGAAAGGG GTTGACGGCC GTACCGGAGG GTCTCAGCGC CTTCACCCAA 
GCACTGGATA TCAGTATGAA CAATATCACC CAGTTACCAG AAGATGCATT TAAGAGTTTC 
CCATTTCTAG AGGAGCTACA ACTGGCTGGT AACGACCTTT CTCTTATCCA TCCAAAAGCC 
TTGTCTGGGC TGAAAGAACT CAAAGTCCTA ACACTCCAGA ATAATCAGTT GAGAACAGTG 
CCCAGTGAAG CCATTCACGG ACTGAGTGCT TTGCAGTCTT TACGCTTAGA TGCCAACCAT 
ATTACCTCAG TCCCGGAGGA CAGTTTTGAA GGGCTTGTCC AGTTACGCCA TCTGTGGCTG 
GATGACAACA GCTTGACGGA AGTGCCCGTG CGTCCCCTCA GCAACCTGCC AACCCTGCAG 
GCGCTGACCT TGGCTCTCAA CAACATCTCA AGCATCCCTG ACTTCGCTTT CACCAACCTT 
TCAAGCTTGG TGGTTCTGCA TCTGCATAAC AATAAAATTA AAAGCCTCAG TCAACACTGT 
TTTGATGGAC TAGATAACCT GGAAACCTTG GACTTGAATT ACAATTACTT GGATGAGTTT 
CCTCAGGCTA TTAAAGCCCT TCCCAGCCTT AAAGAGCTGG GATTTCACAG TAATTCTATT 
TCTGTTATTC CTGATGGAGC ATTTGGTGGT AATCCACTGC TAAGAACTAT TCATTTGTAT 
GATAATCCTC TGTCTTTTGT GGGGAACTCA GCATTTCACA ACCTGTCTGA TCTGCATTGC 
TTAGTCATTC GTGGTGCAAG CCTGGTGCAG TGGTTCCCCA ATCTGACCGG AACTGTCCAT 
TTGGAGAGTC TAACCTTGAC AGGGACAAAA ATAAGCAGCA TACCTGATGA TCTGTGCCAA 
AACCAAAAGA TGCTGAGGAC TCTGGACTTA TCTTATAACA ATATAAGAGA CCTTCCAAGT 
TTTAATGGTT GTCGTGCATT GGAAGAAATT TCATTGCAGC GTAATCAAAT CTCCCTAATA 
AAGGAAAATA CTTTTCAAGG CCTAACATCT CTAAGGATTC TAGATCTGAG TAGAAACCTG 
ATCCGTGAAA TTCACAGTGG AGCTTTTGCG AAGCTTGGGA CAATTACTAA CCTGGATGTA 
AGTTTCAATG AATTAACTTC ATTTCCTACG GAAGGCCTAA ATGGGCTCAA TCAACTAAAG 
CTTGTGGGTA ACTTCAAGCT GAAAGACGCC TTGGCAGCCA GAGACTTTGC TAATCTCAGG 
TCTCTATCAG TACCATATGC TTATCAGTGT TGTGCATTTT GGGGGTGTGA CTCTTATGCA 
AATTTAAACA CAGAAGATAA CAGCCCCCAA GAACACAGTG TGACAAAAGA GAAAGGTGCT 
ACAGATGCAG CAAATGTCAC CAGCACTGCT GAGAACGAAG AACATAGCCA AATAATTATC 
CACTGTACAC CTTCAACAGG TGCTTTCAAG CCCTGTGAAT ATTTACTGGG AAGCTGGATG 
ATTCGCCTTA CAGTGTGGTT CATTTTCCTG GTCGCCTTGC TTTTCAACCT GCTTGTCATT 
TTAACAGTGT TTGCGTCTTG TTCATCACTG CCTGCCTCCA AACTCTTCAT AGGCTTGATT 
TCTGTGTCTA ACTTACTCAT GGGCATCTAT ACTGGCATCC TTACTTTTCT TGATGCTGTG 
TCCTGGGGCC GATTTGCCGA ATTTGGCATT TGGTGGGAAA CTGGCAGCGG CTGCAAGGTA 
GCCGGGTCTC TGGCAGTCTT CTCCTCAGAG AGCGCTGTAT TCCTATTAAC ACTGGCAGCT 
GTGGAAAGAA GCGTATTTGC AAAGGATTTG ATGAAACACG GGAAGAGCAG TCACCTCAGA 
CAGTTCCAGG TGGCCGCCCT CTTAGCTTTG CTGGGTGCCG CAGTGGCAGG CTGCTTCCCC 
CTTTTCCACG GAGGGCAATA TTCTGCATCG CCCTTGTGTT TGCCGTTTCC TACAGGAGAA 
ACCCCATCGT TAGGATTCAC TGTGACCTTA GTGCTATTAA ACTCACTGGC ATTTTTACTA 
ATGGCCATTA TCTACACTAA ACTATACTGC AACTTAGAGA AGGAGGACCT GTCGGAAAAC 
TCCCAGTCTA GCGTGATTAA GCACGTTGCC TGGCTCATCT TCACAAACTG CATCTTCTTC 
TGCCCTGTTG CATTTTTCTC ATTTGCACCA TTGATCACGG CAATCTCCAT CAGCCCCGAG 
ATAATGAAGT CTGTTACACT GATATTCTTC CCGTTGCCTG CTTGCCTGAA TCCGGTCCTG 
TATGTTTTCT TCAACCCAAA GTTTAAAGAA GACTGGAAGC TACTGAAGCG GCGTGTTACC 
AGGAAACACG GATCTGTTTC AGTTTCCATC AGCAGCCAAG GCGGTTGTGG GGAACAGGAT 
TTCTACTATG ACTGTGGCAT GTATTCCCAC TTGCAGGGTA ACCTGACTGT CTGTGACTGC 
TGTGAGTCAT TTCTTTTGAC AAAACCAGTA TCATGCAAAC ACTTAATAAA ATCGCACAGT 
TGTCCTGTAT TGACAGCGGC CTCTTGCCAG AGGCCAGAGG CCTACTGGTC TGATTGTGGT 
ACACAGTCAG CCCATTCTGA CTATGCAGAT GAAGAAGATT CCTTTGTCTC AGACAGCTCT 
GACCAGGTGC AGGCCTGTGG ACGAGCCTGC TTCTACCAGA GTCGTGGATT CCCTCTGGTG 
CGCTATGCTT ACAATCTACA GAGAGTCAGA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin development in mice.
Developmental biology390(2)181-90(2014 Jun)
Kinzel B,Pikiolek M,Orsini V,Sprunger J,Isken A,Zietzling S,Desplanches M,Dubost V,Breustedt D,Valdez R,Liu D,Theil D,M?ller M,Dietrich B,Bouwmeester T,Ruffner H,Tchorz JS

LGR4 acts as a link between the peripheral circadian clock and lipid metabolism in liver.
Journal of molecular endocrinology52(2)133-43(2014 Apr)
Wang F,Zhang X,Wang J,Chen M,Fan N,Ma Q,Liu R,Wang R,Li X,Liu M,Ning G

LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats.
Endocrinology155(2)429-40(2014 Feb)
Li JY,Chai B,Zhang W,Fritze DM,Zhang C,Mulholland MW

A naturally occurring Lgr4 splice variant encodes a soluble antagonist useful for demonstrating the gonadal roles of Lgr4 in mammals.
PloS one9(9)e106804(2014)
Hsu PJ,Wu FJ,Kudo M,Hsiao CL,Hsueh AJ,Luo CW

Ablation of LGR4 promotes energy expenditure by driving white-to-brown fat switch.
Nature cell biology15(12)1455-63(2013 Dec)
Wang J,Liu R,Wang F,Hong J,Li X,Chen M,Ke Y,Zhang X,Ma Q,Wang R,Shi J,Cui B,Gu W,Zhang Y,Zhang Z,Wang W,Xia X,Liu M,Ning G

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Lgr4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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