Krt20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Krt20
Entrez Gene ID	286912
Full Name	keratin 20
Synonyms	Krt21
General protein information	Preferred Names keratin 20 Names keratin, type I cytoskeletal 20 CK-20 CK-21 K20 cytokeratin-20 cytokeratin-21 keratin 20, type I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	novel type I cytokeratin which expresses immunologically specific fusion protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_173128.1	NP_775151.1	keratin, type I cytoskeletal 20

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KRT20	XP_001168931.1
Bos taurus (cattle)	KRT20	NP_001095321.1
Homo sapiens (human)	KRT20	NP_061883.1
Canis lupus familiaris (dog)	KRT20	XP_850091.1
Rattus norvegicus (Norway rat)	Krt20	NP_775151.1
Gallus gallus (chicken)	KRT20	NP_001264910.1
	KRT20	XP_001101772.2
Mus musculus (house mouse)	Krt20	NP_075745.1

Gene Ontology
Bibliography

Related articles in PubMed

Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker.
Zhou Q, Cadrin M, Herrmann H, Chen CH, Chalkley RJ, Burlingame AL, Omary MB
The Journal of biological chemistry281(24)16453-61(2006 Jun)

Keratin 20 helps maintain intermediate filament organization in intestinal epithelia.
Zhou Q, Toivola DM, Feng N, Greenberg HB, Franke WW, Omary MB
Molecular biology of the cell14(7)2959-71(2003 Jul)

An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium.
Barrett AW, Cort EM, Patel P, Berkovitz BK
Archives of oral biology45(10)879-87(2000 Oct)

Cytokeratin profile relates to histological subtypes and intrahepatic location of intrahepatic cholangiocarcinoma and primary sites of metastatic adenocarcinoma of liver.
Shimonishi T, Miyazaki K, Nakanuma Y
Histopathology37(1)55-63(2000 Jul)

Identification and characterization of rat intestinal keratins. Molecular cloning of cDNAs encoding cytokeratins 8, 19, and a new 49-kDa type I cytokeratin (cytokeratin 21) expressed by differentiated intestinal epithelial cells.
Chandler JS, Calnek D, Quaroni A
The Journal of biological chemistry266(18)11932-8(1991 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Krt20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Krt20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03500	NM_173128.1 Latest version!	Rattus norvegicus keratin 20 (Krt20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Krt20 ( NM_173128.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775151.1 Rattus norvegicus keratin 20 (Krt20), mRNA.

Price & Availability↑

CloneID	ORa03500
Clone ID Related Accession (Same CDS sequence)	NM_173128.1
Accession Version	NM_173128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	keratin, type I cytoskeletal 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M63665.1. On Dec 17, 2003 this sequence version replaced XM_346404.1. ##Evidence-Data-START## Transcript exon combination :: M63665.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01906351, SAMN01906356 [ECO:0000350] ##Evidence-Data-END##

ATGGACTTCA GTCGTCGAAG TTTCCACAGA AGTCTGAGTT CCTCCTCACA AGGCCCAGCA 
CTCAGCACGA GTGGCTCCCT GTATAGAAAG GGGACCATGC AGCGTCTGGG GTTGCACAGC 
GTCTACGGTG GCTGGAGGCA CGGCACCCGC ATCTCTGTCT CCAAAACCAC AATGAGCTAC 
GGGAACCATC TCTCCAACGG AGGGGACCTG TTTGGGGGCA ATGAGAAATT GGCAATGCAG 
AACCTCAATG ACAGATTGGC AAGCTACCTA GAAAAAGTGC GCTCCCTGGA GCAATCCAAT 
TCCAAACTTG AAGCCCAGAT CAAGCAGTGG TACGAAACCA ATGCACCGAG CACCATCCGG 
GACTACAGTT CCTACTACGC ACAGATCAAA GAGCTGCAAG ATCAGATTAA GGATGCTCAA 
ATAGAAAACG CCCGATGCGT CTTGCAAATT GACAATGCTA AGCTGGCCGC AGAGGACTTC 
AGATTGAAGT TTGAGACTGA AAGGGGCATG CGGATAACTG TGGAAGCCGA TCTCCAAGGC 
CTGAGCAAGG TCTATGATGA CCTGACCCTT CAAAAGACAG ACTTGGAAAT TCAGATTGAA 
GAACTGAACA AGGACCTGGC CCTCCTCAAA AAAGAGCATC AAGAGGAAGT CGAGGTCCTT 
CGCCGGCAGC TGGGCAACAA TGTCAACGTG GAGGTGGATG CCGCTCCAGG CCTGAACCTG 
GGTGAGATCA TGAATGAGAT GAGACAGAAA TATGAAATCC TGGCCCAGAA GAACCTGCAG 
GAGGCCAAAG AACAGTTTGA GAGACAGACT CAAACTCTGG AGAAACAAGT CACAGTGAAC 
ATTGAGGAGC TGAGAGGAAC CGAGGTTCAA GTCACGGAAC TGAGACGCAG CTACCAGACC 
CTGGAAATAG AGCTCCAGTC CCAACTAAGC ATGAAAGAGT CTCTGGAGCG CACCCTGGAG 
GAGACCAAGG CTCGTTATGC CAGCCAGTTG GCGGCCATCC AGGAAATGCT GAGTTCCCTG 
GAGGCCCAAC TGATGCAGAT CCGGAGCGAC ACTGAACGCC AGAACCAAGA GTACAATATC 
CTCCTTGACA TAAAGACCCG GCTTGAGCAG GAGATCGCCA CCTACCGCCG CCTTCTGGAA 
GGAGAGGACA TAAAAACTAC AGAGTACCAG CTAAACACTT TGGAAGCAAA AGATATCAAG 
AAAACCAGGA AGATTAAGAC GGTCGTGGAG GAAGTGGTAG ACGGCAAGGT CGTGTCCTCT 
GAAGTCAAGG AGATAGAAGA AAACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775151.1
CDS	16..1305
Translation	MDFSRRSFHRSLSSSSQGPALSTSGSLYRKGTMQRLGLHSVYGGWRHGTRISVSKTTMSYGNHLSNGGDLFGGNEKLAMQNLNDRLASYLEKVRSLEQSNSKLEAQIKQWYETNAPSTIRDYSSYYAQIKELQDQIKDAQIENARCVLQIDNAKLAAEDFRLKFETERGMRITVEADLQGLSKVYDDLTLQKTDLEIQIEELNKDLALLKKEHQEEVEVLRRQLGNNVNVEVDAAPGLNLGEIMNEMRQKYEILAQKNLQEAKEQFERQTQTLEKQVTVNIEELRGTEVQVTELRRSYQTLEIELQSQLSMKESLERTLEETKARYASQLAAIQEMLSSLEAQLMQIRSDTERQNQEYNILLDIKTRLEQEIATYRRLLEGEDIKTTEYQLNTLEAKDIKKTRKIKTVVEEVVDGKVVSSEVKEIEENI

Target ORF information:

RefSeq Version	NM_173128.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus keratin 20 (Krt20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173128.1

ORF Insert Sequence:

ATGGACTTCA GTCGTCGAAG TTTCCACAGA AGTCTGAGTT CCTCCTCACA AGGCCCAGCA 
CTCAGCACGA GTGGCTCCCT GTATAGAAAG GGGACCATGC AGCGTCTGGG GTTGCACAGC 
GTCTACGGTG GCTGGAGGCA CGGCACCCGC ATCTCTGTCT CCAAAACCAC AATGAGCTAC 
GGGAACCATC TCTCCAACGG AGGGGACCTG TTTGGGGGCA ATGAGAAATT GGCAATGCAG 
AACCTCAATG ACAGATTGGC AAGCTACCTA GAAAAAGTGC GCTCCCTGGA GCAATCCAAT 
TCCAAACTTG AAGCCCAGAT CAAGCAGTGG TACGAAACCA ATGCACCGAG CACCATCCGG 
GACTACAGTT CCTACTACGC ACAGATCAAA GAGCTGCAAG ATCAGATTAA GGATGCTCAA 
ATAGAAAACG CCCGATGCGT CTTGCAAATT GACAATGCTA AGCTGGCCGC AGAGGACTTC 
AGATTGAAGT TTGAGACTGA AAGGGGCATG CGGATAACTG TGGAAGCCGA TCTCCAAGGC 
CTGAGCAAGG TCTATGATGA CCTGACCCTT CAAAAGACAG ACTTGGAAAT TCAGATTGAA 
GAACTGAACA AGGACCTGGC CCTCCTCAAA AAAGAGCATC AAGAGGAAGT CGAGGTCCTT 
CGCCGGCAGC TGGGCAACAA TGTCAACGTG GAGGTGGATG CCGCTCCAGG CCTGAACCTG 
GGTGAGATCA TGAATGAGAT GAGACAGAAA TATGAAATCC TGGCCCAGAA GAACCTGCAG 
GAGGCCAAAG AACAGTTTGA GAGACAGACT CAAACTCTGG AGAAACAAGT CACAGTGAAC 
ATTGAGGAGC TGAGAGGAAC CGAGGTTCAA GTCACGGAAC TGAGACGCAG CTACCAGACC 
CTGGAAATAG AGCTCCAGTC CCAACTAAGC ATGAAAGAGT CTCTGGAGCG CACCCTGGAG 
GAGACCAAGG CTCGTTATGC CAGCCAGTTG GCGGCCATCC AGGAAATGCT GAGTTCCCTG 
GAGGCCCAAC TGATGCAGAT CCGGAGCGAC ACTGAACGCC AGAACCAAGA GTACAATATC 
CTCCTTGACA TAAAGACCCG GCTTGAGCAG GAGATCGCCA CCTACCGCCG CCTTCTGGAA 
GGAGAGGACA TAAAAACTAC AGAGTACCAG CTAAACACTT TGGAAGCAAA AGATATCAAG 
AAAACCAGGA AGATTAAGAC GGTCGTGGAG GAAGTGGTAG ACGGCAAGGT CGTGTCCTCT 
GAAGTCAAGG AGATAGAAGA AAACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker.
The Journal of biological chemistry281(24)16453-61(2006 Jun)
Zhou Q,Cadrin M,Herrmann H,Chen CH,Chalkley RJ,Burlingame AL,Omary MB

Keratin 20 helps maintain intermediate filament organization in intestinal epithelia.
Molecular biology of the cell14(7)2959-71(2003 Jul)
Zhou Q,Toivola DM,Feng N,Greenberg HB,Franke WW,Omary MB

An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium.
Archives of oral biology45(10)879-87(2000 Oct)
Barrett AW,Cort EM,Patel P,Berkovitz BK

Cytokeratin profile relates to histological subtypes and intrahepatic location of intrahepatic cholangiocarcinoma and primary sites of metastatic adenocarcinoma of liver.
Histopathology37(1)55-63(2000 Jul)
Shimonishi T,Miyazaki K,Nakanuma Y

Identification and characterization of rat intestinal keratins. Molecular cloning of cDNAs encoding cytokeratins 8, 19, and a new 49-kDa type I cytokeratin (cytokeratin 21) expressed by differentiated intestinal epithelial cells.
The Journal of biological chemistry266(18)11932-8(1991 Jun)
Chandler JS,Calnek D,Quaroni A