Eif2ak1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif2ak1
Entrez Gene ID	27137
Full Name	eukaryotic translation initiation factor 2 alpha kinase 1
Synonyms	Hri
General protein information	Preferred Names eukaryotic translation initiation factor 2 alpha kinase 1 Names eukaryotic translation initiation factor 2-alpha kinase 1 HCR heme-controlled repressor heme-regulated eukaryotic initiation factor eIF-2-alpha kinase heme-regulated inhibitor hemin-sensitive initiation factor 2-alpha kinase hemin-sensitive initiation factor 2a kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11
Summary	protein kinase, negatively regulated by hemin; may play an important role in translational control of protein synthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013223.1	NP_037355.1	eukaryotic translation initiation factor 2-alpha kinase 1

Pathways from BioSystems

Homologies

	EIF2AK1	XP_001089983.2
Canis lupus familiaris (dog)	EIF2AK1	NP_001155185.1
Rattus norvegicus (Norway rat)	Eif2ak1	NP_037355.1
Danio rerio (zebrafish)	eif2ak1	NP_001071035.1
Schizosaccharomyces pombe (fission yeast)	hri2	NP_593146.1
Gallus gallus (chicken)	EIF2AK1	NP_989979.1
Pan troglodytes (chimpanzee)	EIF2AK1	XP_518962.3
Homo sapiens (human)	EIF2AK1	NP_001127807.1
Mus musculus (house mouse)	Eif2ak1	NP_038585.2
Xenopus tropicalis (tropical clawed frog)	eif2ak1	NP_001039038.1
Bos taurus (cattle)	EIF2AK1	NP_001033142.1
Schizosaccharomyces pombe (fission yeast)	hri1	NP_594738.1

Gene Ontology
Bibliography

Related articles in PubMed

Hepatic heme-regulated inhibitor (HRI) eukaryotic initiation factor 2alpha kinase: a protagonist of heme-mediated translational control of CYP2B enzymes and a modulator of basal endoplasmic reticulum stress tone.
Acharya P, Chen JJ, Correia MA
Molecular pharmacology77(4)575-92(2010 Apr)

The function of heme-regulated eIF2alpha kinase in murine iron homeostasis and macrophage maturation.
Liu S, Suragani RN, Wang F, Han A, Zhao W, Andrews NC, Chen JJ
The Journal of clinical investigation117(11)3296-305(2007 Nov)

Heme-regulated eIF2alpha kinase modifies the phenotypic severity of murine models of erythropoietic protoporphyria and beta-thalassemia.
Han AP, Fleming MD, Chen JJ
The Journal of clinical investigation115(6)1562-70(2005 Jun)

Heme-regulated eIF2alpha kinase (HRI) is required for translational regulation and survival of erythroid precursors in iron deficiency.
Han AP, Yu C, Lu L, Fujiwara Y, Browne C, Chin G, Fleming M, Leboulch P, Orkin SH, Chen JJ
The EMBO journal20(23)6909-18(2001 Dec)

Multiple autophosphorylation is essential for the formation of the active and stable homodimer of heme-regulated eIF2alpha kinase.
Bauer BN, Rafie-Kolpin M, Lu L, Han A, Chen JJ
Biochemistry40(38)11543-51(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

eukaryotic initiation factor 2alpha kinase is a protagonist of heme-mediated translational control of CYP2B enzymes and a modulator of basal endoplasmic reticulum stress tone.
Title: Hepatic heme-regulated inhibitor (HRI) eukaryotic initiation factor 2alpha kinase: a protagonist of heme-mediated translational control of CYP2B enzymes and a modulator of basal endoplasmic reticulum stress tone.

The following Eif2ak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2ak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02381	NM_013223.1 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif2ak1 ( NM_013223.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037355.1 Rattus norvegicus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), mRNA.

Price & Availability↑

CloneID	ORa02381
Clone ID Related Accession (Same CDS sequence)	NM_013223.1
Accession Version	NM_013223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic translation initiation factor 2-alpha kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L27707.1. ##Evidence-Data-START## Transcript exon combination :: L27707.1, BC081838.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGGGG GCGGCTCCGT GGATGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG 
GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG 
TCCGATGTCC CAGCAGAGCT CCAAGTGTTC AAAGAGCCCT TGCAGCAGCC CACGTTCCCT 
TTCTTAGTGG CAAACCAGCT GCTGCTGGTC TCCTTGCTGG AACACTTGAG CCATGTGCAC 
GAGCCGAACC CACTTCACTC AAAACAGGTC TTTAAATTAC TGTGCCAGAC TTTTATCAAG 
ATGGGGCTGC TCTCTTCCTT TACCTGCAGC GATGAGTTCA GCTCCCTGAG ACTCCACCAC 
AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAGGAGA GAGTCCGTCA GGATCCTTGT 
CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGGGAGA TAGCTCTCGA AGCACAGACT 
TCACGCTACT TAAATGAATT TGAAGAGCTC GCCATCTTAG GAAAAGGCGG ATATGGAAGA 
GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT 
AAGAGTGCAA CTAAAACGGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGC 
CTCCAGCACC CCAATATCGT TGGCTACCAC ACTGCATGGA TAGAGCACGT TCACGTGCTT 
CAGCCACAAG ACAGAGTTCC CATTCAACTG CCCTCTCTTG AAGTGTTGTC AGAGCATGAA 
GGGGACAGAA ATCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TATCTTTGCT 
GAACTCACCC CAGAAAAAGA AAACCCTTTA GCGGAATCTG ATGTTAAGAA TGAGAATAAC 
AACTTGGTGA GCTACAGGGC CAACTTAGTG ATCAGGAGCA GCAGTGAAAG CGAATCGTCC 
ATTGAGCTCC AAGAAGATGG CTTGAACGAG TCGCCTCTCA GACCAGTTGT CAAGCACCAG 
CTGCCGCTGG GGCATAGCTC AGACGTGGAA GGGAATTTTA CCTCCACGGA TGAGTCTTCT 
GAAGACAATT TGAACCTGCT GGGGCAGACA GAGGCGCGGT ACCACCTGAT GCTGCACATC 
CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAG CTGAGAGGAA CAAGCGGAGC 
CGGAAGTGCG TGGATGAAGC AGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAGATT 
TTTCAAGAAC TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATCGT CCACAGAGAT 
CTGAAGCCTA GAAATATTTT TCTTCATGGT CCTGATCAAC AAGTGAAAAT AGGAGACTTT 
GGTCTGGCCT GTGCAGACAT CATTCAGAAG AGCGCAGATT GGACCAACAG AAACGGGAAA 
GGAACGCCGA CACACACGTC CCGAGTGGGG ACTTGTCTCT ACGCGTCACC TGAGCAGTTG 
GAAGGATCCG AGTATGATGC CAAGTCTGAT ATGTACAGCT TGGGTGTGAT CCTGCTCGAG 
CTCTTCCAGC CATTCGGGAC AGAAATGGAG CGAGCAACAG TCCTAACAGG TGTGAGGACT 
GGTCGGATAC CAGAGTCCCT CAGTAAAAGG TGTCCAGTGC AAGCCAAGTA TATCCAGCTC 
CTGACTGGGA GGAACGCGGC CCAGAGACCA TCTGCTCTTC AGCTACTGCA GAGCGAACTG 
TTCCAAACAA CGGGAAATGT TAATCTCACA TTGCAGATGA AGATAATGGA GCAAGAAAAG 
GAAATTGAAG AATTAAAGAA GCAACTAAGC CTCCTTTCGC AGGACAAAGG GCTGAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037355.1
CDS	13..1875
Translation	MLGGGSVDGERDTDDDAAGAVAAPPAIDFPAEVSDPKYDESDVPAELQVFKEPLQQPTFPFLVANQLLLVSLLEHLSHVHEPNPLHSKQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPCQDNSYMQKIRSREIALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSATKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVLQPQDRVPIQLPSLEVLSEHEGDRNQGGVKDNESSSSIIFAELTPEKENPLAESDVKNENNNLVSYRANLVIRSSSESESSIELQEDGLNESPLRPVVKHQLPLGHSSDVEGNFTSTDESSEDNLNLLGQTEARYHLMLHIQMQLCELSLWDWIAERNKRSRKCVDEAACPYVMASVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACADIIQKSADWTNRNGKGTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVILLELFQPFGTEMERATVLTGVRTGRIPESLSKRCPVQAKYIQLLTGRNAAQRPSALQLLQSELFQTTGNVNLTLQMKIMEQEKEIEELKKQLSLLSQDKGLKR

Target ORF information:

RefSeq Version	NM_013223.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 2 alpha kinase 1 (Eif2ak1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013223.1

ORF Insert Sequence:

ATGCTGGGGG GCGGCTCCGT GGATGGCGAG CGCGACACGG ACGACGACGC GGCGGGGGCG 
GTGGCCGCGC CTCCTGCCAT CGACTTCCCC GCAGAGGTGT CGGACCCCAA GTATGATGAG 
TCCGATGTCC CAGCAGAGCT CCAAGTGTTC AAAGAGCCCT TGCAGCAGCC CACGTTCCCT 
TTCTTAGTGG CAAACCAGCT GCTGCTGGTC TCCTTGCTGG AACACTTGAG CCATGTGCAC 
GAGCCGAACC CACTTCACTC AAAACAGGTC TTTAAATTAC TGTGCCAGAC TTTTATCAAG 
ATGGGGCTGC TCTCTTCCTT TACCTGCAGC GATGAGTTCA GCTCCCTGAG ACTCCACCAC 
AACAGAGCCA TCACTCATTT AATGAGGTCT GCCAAGGAGA GAGTCCGTCA GGATCCTTGT 
CAAGATAATT CTTACATGCA GAAAATCAGA TCCAGGGAGA TAGCTCTCGA AGCACAGACT 
TCACGCTACT TAAATGAATT TGAAGAGCTC GCCATCTTAG GAAAAGGCGG ATATGGAAGA 
GTTTACAAGG TCCGGAACAA ATTAGATGGT CAGCATTATG CAATTAAGAA AATCCTGATT 
AAGAGTGCAA CTAAAACGGA TTGTATGAAG GTGCTACGGG AAGTGAAGGT TCTGGCAGGC 
CTCCAGCACC CCAATATCGT TGGCTACCAC ACTGCATGGA TAGAGCACGT TCACGTGCTT 
CAGCCACAAG ACAGAGTTCC CATTCAACTG CCCTCTCTTG AAGTGTTGTC AGAGCATGAA 
GGGGACAGAA ATCAAGGTGG TGTTAAAGAT AATGAAAGCA GTTCGTCCAT TATCTTTGCT 
GAACTCACCC CAGAAAAAGA AAACCCTTTA GCGGAATCTG ATGTTAAGAA TGAGAATAAC 
AACTTGGTGA GCTACAGGGC CAACTTAGTG ATCAGGAGCA GCAGTGAAAG CGAATCGTCC 
ATTGAGCTCC AAGAAGATGG CTTGAACGAG TCGCCTCTCA GACCAGTTGT CAAGCACCAG 
CTGCCGCTGG GGCATAGCTC AGACGTGGAA GGGAATTTTA CCTCCACGGA TGAGTCTTCT 
GAAGACAATT TGAACCTGCT GGGGCAGACA GAGGCGCGGT ACCACCTGAT GCTGCACATC 
CAGATGCAGC TGTGTGAGCT CTCCCTGTGG GACTGGATAG CTGAGAGGAA CAAGCGGAGC 
CGGAAGTGCG TGGATGAAGC AGCTTGTCCC TATGTTATGG CCAGTGTTGC AACAAAGATT 
TTTCAAGAAC TGGTGGAAGG TGTCTTTTAC ATACATAACA TGGGCATCGT CCACAGAGAT 
CTGAAGCCTA GAAATATTTT TCTTCATGGT CCTGATCAAC AAGTGAAAAT AGGAGACTTT 
GGTCTGGCCT GTGCAGACAT CATTCAGAAG AGCGCAGATT GGACCAACAG AAACGGGAAA 
GGAACGCCGA CACACACGTC CCGAGTGGGG ACTTGTCTCT ACGCGTCACC TGAGCAGTTG 
GAAGGATCCG AGTATGATGC CAAGTCTGAT ATGTACAGCT TGGGTGTGAT CCTGCTCGAG 
CTCTTCCAGC CATTCGGGAC AGAAATGGAG CGAGCAACAG TCCTAACAGG TGTGAGGACT 
GGTCGGATAC CAGAGTCCCT CAGTAAAAGG TGTCCAGTGC AAGCCAAGTA TATCCAGCTC 
CTGACTGGGA GGAACGCGGC CCAGAGACCA TCTGCTCTTC AGCTACTGCA GAGCGAACTG 
TTCCAAACAA CGGGAAATGT TAATCTCACA TTGCAGATGA AGATAATGGA GCAAGAAAAG 
GAAATTGAAG AATTAAAGAA GCAACTAAGC CTCCTTTCGC AGGACAAAGG GCTGAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hepatic heme-regulated inhibitor (HRI) eukaryotic initiation factor 2alpha kinase: a protagonist of heme-mediated translational control of CYP2B enzymes and a modulator of basal endoplasmic reticulum stress tone.
Molecular pharmacology77(4)575-92(2010 Apr)
Acharya P,Chen JJ,Correia MA

The function of heme-regulated eIF2alpha kinase in murine iron homeostasis and macrophage maturation.
The Journal of clinical investigation117(11)3296-305(2007 Nov)
Liu S,Suragani RN,Wang F,Han A,Zhao W,Andrews NC,Chen JJ

Heme-regulated eIF2alpha kinase modifies the phenotypic severity of murine models of erythropoietic protoporphyria and beta-thalassemia.
The Journal of clinical investigation115(6)1562-70(2005 Jun)
Han AP,Fleming MD,Chen JJ

Heme-regulated eIF2alpha kinase (HRI) is required for translational regulation and survival of erythroid precursors in iron deficiency.
The EMBO journal20(23)6909-18(2001 Dec)
Han AP,Yu C,Lu L,Fujiwara Y,Browne C,Chin G,Fleming M,Leboulch P,Orkin SH,Chen JJ

Multiple autophosphorylation is essential for the formation of the active and stable homodimer of heme-regulated eIF2alpha kinase.
Biochemistry40(38)11543-51(2001 Sep)
Bauer BN,Rafie-Kolpin M,Lu L,Han A,Chen JJ

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Eif2ak1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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