Gpr3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr3
Entrez Gene ID	266769
Full Name	G protein-coupled receptor 3
Synonyms	Gpcr3
General protein information	Preferred Names G protein-coupled receptor 3 Names G-protein coupled receptor 3 G protein-coupled receptor R4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	one of several G-protein coupled receptors for the lysophospholipid ligand sphingosine 1-phosphate, activated adenylate cyclase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239006.3	XP_006239068.1	G-protein coupled receptor 3 isoform X1
NM_153727.1	NP_714949.1	G-protein coupled receptor 3

Pathways from BioSystems

Homologies

	GPR3	XP_001111240.1
Canis lupus familiaris (dog)	GPR3	XP_544470.1
Rattus norvegicus (Norway rat)	Gpr3	NP_714949.1
Homo sapiens (human)	GPR3	NP_005272.1
Mus musculus (house mouse)	Gpr3	NP_032180.1
Pan troglodytes (chimpanzee)	GPR3	XP_001148989.1
Bos taurus (cattle)	GPR3	XP_005203355.1

Gene Ontology
Bibliography

Related articles in PubMed

Mice lacking GPR3 receptors display late-onset obese phenotype due to impaired thermogenic function in brown adipose tissue.
Godlewski G, Jourdan T, Szanda G, Tam J, Cinar R, Harvey-White J, Liu J, Mukhopadhyay B, Pacher P, Ming Mo F, Osei-Hyiaman D, Kunos G
Scientific reports514953(2015 Oct)

Developmental expression of GPR3 in rodent cerebellar granule neurons is associated with cell survival and protects neurons from various apoptotic stimuli.
Tanaka S, Miyagi T, Dohi E, Seki T, Hide I, Sotomaru Y, Saeki Y, Antonio Chiocca E, Matsumoto M, Sakai N
Neurobiology of disease68215-27(2014 Aug)

Immunolocalization and expression pattern of Gpr3 in the ovary and its effect on proliferation of ovarian granulosa cells in pigs.
Zhang B, Wei Q, Shi S, Dong F, Shi F, Xu Y
The Journal of reproduction and development58(4)410-9(2012)

The G protein coupled receptor 3 is involved in cAMP and cGMP signaling and maintenance of meiotic arrest in porcine oocytes.
Yang CR, Wei Y, Qi ST, Chen L, Zhang QH, Ma JY, Luo YB, Wang YP, Hou Y, Schatten H, Liu ZH, Sun QY
PloS one7(6)e38807(2012)

The G-protein-coupled receptors GPR3 and GPR12 are involved in cAMP signaling and maintenance of meiotic arrest in rodent oocytes.
Hinckley M, Vaccari S, Horner K, Chen R, Conti M
Developmental biology287(2)249-61(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrate for the first time that the expression of GPR3 modulates the viability of cultured P7 rat CGNs and mediates an antiapoptotic effect in various apoptosis-inducing models using rat cerebellar granule neurons.
Title: Developmental expression of GPR3 in rodent cerebellar granule neurons is associated with cell survival and protects neurons from various apoptotic stimuli.

the cAMP levels required for maintaining meiotic arrest in mouse and rat oocytes are dependent on the expression of Gpr3 and/or Gpr12
Title: The G-protein-coupled receptors GPR3 and GPR12 are involved in cAMP signaling and maintenance of meiotic arrest in rodent oocytes.

The following Gpr3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa28680	XM_006239006.3 Latest version!	Rattus norvegicus G protein-coupled receptor 3 (Gpr3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa42347	NM_153727.1 Latest version!	Rattus norvegicus G protein-coupled receptor 3 (Gpr3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr3 ( XM_006239006.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239068.1 Rattus norvegicus G protein-coupled receptor 3 (Gpr3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28680
Clone ID Related Accession (Same CDS sequence)	XM_006239006.3
Accession Version	XM_006239006.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGGG GTGCAGGAAG ATCCATGGCC TGGTTCTCAG CCGGCTCAGG CAGTGTGAAT 
GTGAGCATAG ACCCAGCAGA GGAACCTACA GGCCCAGCTA CACTGCTGCC CTCTCCCAGG 
GCCTGGGATG TGGTGCTGTG CATCTCAGGC ACCCTGGTGT CCTGCGAGAA TGCTCTGGTG 
GTGGCCATCA TTGTGGGCAC GCCTGCCTTC CGCGCCCCCA TGTTCCTGCT GGTGGGCAGC 
TTGGCCGTAG CAGACCTGCT GGCAGGCCTG GGCCTGGTCC TGCACTTCGC TGCTGACTTC 
TGTATTGGCT CACCAGAGAT GAGCTTGGTG CTGGTTGGCG TGCTAGCAAC GGCCTTTACT 
GCCAGCATCG GCAGCCTGCT GGCCATCACC GTTGACCGCT ACCTTTCCCT GTACAACGCC 
CTCACCTACT ACTCAGAGAC AACAGTAACT CGAACCTACG TGATGCTGGC CTTGGTGTGG 
GTGGGTGCCC TGGGCCTGGG GCTGGTTCCC GTGCTGGCCT GGAACTGCCG GGATGGCCTG 
ACCACGTGCG GCGTAGTCTA TCCACTCTCC AAGAACCATC TGGTGGTTCT GGCCATCGTC 
TTCTTCATGG TGTTTGGCAT CATGCTACAG CTCTATGCCC AGATCTGCCG CATTGTTTGC 
CGCCACGCCC AGCAGATCGC CCTCCAACGA CACCTGCTGC CTGCCTCCCA CTACGTGGCC 
ACCCGCAAGG GCATCGCCAC ATTGGCCGTG GTGCTTGGCG CCTTTGCCGC CTGTTGGTTG 
CCCTTCACTG TCTACTGCCT CCTGGGAGAC GCCAACTCTC CTCCTCTCTA CACCTACCTT 
ACCCTGCTCC CTGCCACTTA CAACTCCATG ATCAACCCGG TCATTTACGC CTTCCGCAAC 
CAAGACGTGC AGAAGGTGCT GTGGGCCATC TGCTGCTGCT GTTCTACTTC CAAGATCCCA 
TTCCGGTCCC GGTCCCCTAG TGATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239068.1
CDS	126..1115
Translation	MMWGAGRSMAWFSAGSGSVNVSIDPAEEPTGPATLLPSPRAWDVVLCISGTLVSCENALVVAIIVGTPAFRAPMFLLVGSLAVADLLAGLGLVLHFAADFCIGSPEMSLVLVGVLATAFTASIGSLLAITVDRYLSLYNALTYYSETTVTRTYVMLALVWVGALGLGLVPVLAWNCRDGLTTCGVVYPLSKNHLVVLAIVFFMVFGIMLQLYAQICRIVCRHAQQIALQRHLLPASHYVATRKGIATLAVVLGAFAACWLPFTVYCLLGDANSPPLYTYLTLLPATYNSMINPVIYAFRNQDVQKVLWAICCCCSTSKIPFRSRSPSDV

Target ORF information:

RefSeq Version	XM_006239006.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 3 (Gpr3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239006.3

ORF Insert Sequence:

ATGATGTGGG GTGCAGGAAG ATCCATGGCC TGGTTCTCAG CCGGCTCAGG CAGTGTGAAT 
GTGAGCATAG ACCCAGCAGA GGAACCTACA GGCCCAGCTA CACTGCTGCC CTCTCCCAGG 
GCCTGGGATG TGGTGCTGTG CATCTCAGGC ACCCTGGTGT CCTGCGAGAA TGCTCTGGTG 
GTGGCCATCA TTGTGGGCAC GCCTGCCTTC CGCGCCCCCA TGTTCCTGCT GGTGGGCAGC 
TTGGCCGTAG CAGACCTGCT GGCAGGCCTG GGCCTGGTCC TGCACTTCGC TGCTGACTTC 
TGTATTGGCT CACCAGAGAT GAGCTTGGTG CTGGTTGGCG TGCTAGCAAC GGCCTTTACT 
GCCAGCATCG GCAGCCTGCT GGCCATCACC GTTGACCGCT ACCTTTCCCT GTACAACGCC 
CTCACCTACT ACTCAGAGAC AACAGTAACT CGAACCTACG TGATGCTGGC CTTGGTGTGG 
GTGGGTGCCC TGGGCCTGGG GCTGGTTCCC GTGCTGGCCT GGAACTGCCG GGATGGCCTG 
ACCACGTGCG GCGTAGTCTA TCCACTCTCC AAGAACCATC TGGTGGTTCT GGCCATCGTC 
TTCTTCATGG TGTTTGGCAT CATGCTACAG CTCTATGCCC AGATCTGCCG CATTGTTTGC 
CGCCACGCCC AGCAGATCGC CCTCCAACGA CACCTGCTGC CTGCCTCCCA CTACGTGGCC 
ACCCGCAAGG GCATCGCCAC ATTGGCCGTG GTGCTTGGCG CCTTTGCCGC CTGTTGGTTG 
CCCTTCACTG TCTACTGCCT CCTGGGAGAC GCCAACTCTC CTCCTCTCTA CACCTACCTT 
ACCCTGCTCC CTGCCACTTA CAACTCCATG ATCAACCCGG TCATTTACGC CTTCCGCAAC 
CAAGACGTGC AGAAGGTGCT GTGGGCCATC TGCTGCTGCT GTTCTACTTC CAAGATCCCA 
TTCCGGTCCC GGTCCCCTAG TGATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr3 ( NM_153727.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_714949.1 Rattus norvegicus G protein-coupled receptor 3 (Gpr3), mRNA.

Price & Availability↑

CloneID	ORa42347
Clone ID Related Accession (Same CDS sequence)	NM_153727.1
Accession Version	NM_153727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled receptor 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ427482.1. ##Evidence-Data-START## Transcript exon combination :: AJ427482.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCTGGT TCTCAGCCGG CTCAGGCAGT GTGAATGTGA GCATAGACCC AGCAGAGGAA 
CCTACAGGCC CAGCTACACT GCTGCCCTCT CCCAGGGCCT GGGATGTGGT GCTGTGCATC 
TCAGGCACCC TGGTGTCCTG CGAGAATGCT CTGGTGRTGG CCATCATTGT GGGCACGCCT 
GCCTTCCGCG CCCCCATGTT CCTGCTGGTG GGCAGCTTGG CCGTAGCAGA CCTGCTGGCA 
GGCCTGGGCC TGGTCCTGCA CTTCGCTGCT GACTTCTGTA TTGGCTCACC AGAGATGAGC 
TTGGTGCTGG TTGGCGTGCT AGCAACGGCC TTTACTGCCA GCATCGGCAG CCTGCTGGCC 
ATCACCGTTG ACCGCTACCT TTCCCTGTAC AACGCCCTCA CCTACTACTC AGAGACAACA 
GTAACTCGAA CCTACGTGAT GCTGGCCTTG GTGTGGGTGG GTGCCCTGGG CCTGGGGCTG 
GTTCCCGTGC TGGCCTGGAA CTGCCGGGAT GGCCTGACCA CGTGCGGCGT AGTCTATCCA 
CTCTCCAAGA ACCATCTGGT GGTTCTGGCC ATCGTCTTCT TCATGGTGTT TGGCATCATG 
CTACAAGCTC TATGCCCAGA TCTGCCGCAT TGTTTGCCGC CACGGCCCAA GCAGATCGCC 
CTCCAACGAC ACCTGCTGCC TGCCTCCCAC TACGTGGCCA CCCGCAAGGG CATCGCCACA 
TTGGCCGTGG TGCTTGGCGC CTTTGCCGCC TGTTGGTTGC CCTTCACTGT CTACTGCCTC 
CTGGGAGACG CCAACTCTCC TCCTCTCTAC ACCTACCTTA CCCTGCTCCC TGCCACTTAC 
AACTCCATGA TCAACCCGGT CATTTACGCC TTCCGCAACC AAGACGTGCA GAAGGTGCTG 
TGGGCCATCT GCTGCTGCTG TTCTACTTCC AAGATCCCAT TCCGGTCCCG GTCCCCTAGT 
GATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_714949.1
CDS	1..969
Translation	MAWFSAGSGSVNVSIDPAEEPTGPATLLPSPRAWDVVLCISGTLVSCENALVnullAIIVGTPAFRAPMFLLVGSLAVADLLAGLGLVLHFAADFCIGSPEMSLVLVGVLATAFTASIGSLLAITVDRYLSLYNALTYYSETTVTRTYVMLALVWVGALGLGLVPVLAWNCRDGLTTCGVVYPLSKNHLVVLAIVFFMVFGIMLQALCPDLPHCLPPRPKQIALQRHLLPASHYVATRKGIATLAVVLGAFAACWLPFTVYCLLGDANSPPLYTYLTLLPATYNSMINPVIYAFRNQDVQKVLWAICCCCSTSKIPFRSRSPSDV

Target ORF information:

RefSeq Version	NM_153727.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled receptor 3 (Gpr3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153727.1

ORF Insert Sequence:

ATGGCCTGGT TCTCAGCCGG CTCAGGCAGT GTGAATGTGA GCATAGACCC AGCAGAGGAA 
CCTACAGGCC CAGCTACACT GCTGCCCTCT CCCAGGGCCT GGGATGTGGT GCTGTGCATC 
TCAGGCACCC TGGTGTCCTG CGAGAATGCT CTGGTGRTGG CCATCATTGT GGGCACGCCT 
GCCTTCCGCG CCCCCATGTT CCTGCTGGTG GGCAGCTTGG CCGTAGCAGA CCTGCTGGCA 
GGCCTGGGCC TGGTCCTGCA CTTCGCTGCT GACTTCTGTA TTGGCTCACC AGAGATGAGC 
TTGGTGCTGG TTGGCGTGCT AGCAACGGCC TTTACTGCCA GCATCGGCAG CCTGCTGGCC 
ATCACCGTTG ACCGCTACCT TTCCCTGTAC AACGCCCTCA CCTACTACTC AGAGACAACA 
GTAACTCGAA CCTACGTGAT GCTGGCCTTG GTGTGGGTGG GTGCCCTGGG CCTGGGGCTG 
GTTCCCGTGC TGGCCTGGAA CTGCCGGGAT GGCCTGACCA CGTGCGGCGT AGTCTATCCA 
CTCTCCAAGA ACCATCTGGT GGTTCTGGCC ATCGTCTTCT TCATGGTGTT TGGCATCATG 
CTACAAGCTC TATGCCCAGA TCTGCCGCAT TGTTTGCCGC CACGGCCCAA GCAGATCGCC 
CTCCAACGAC ACCTGCTGCC TGCCTCCCAC TACGTGGCCA CCCGCAAGGG CATCGCCACA 
TTGGCCGTGG TGCTTGGCGC CTTTGCCGCC TGTTGGTTGC CCTTCACTGT CTACTGCCTC 
CTGGGAGACG CCAACTCTCC TCCTCTCTAC ACCTACCTTA CCCTGCTCCC TGCCACTTAC 
AACTCCATGA TCAACCCGGT CATTTACGCC TTCCGCAACC AAGACGTGCA GAAGGTGCTG 
TGGGCCATCT GCTGCTGCTG TTCTACTTCC AAGATCCCAT TCCGGTCCCG GTCCCCTAGT 
GATGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mice lacking GPR3 receptors display late-onset obese phenotype due to impaired thermogenic function in brown adipose tissue.
Scientific reports514953(2015 Oct)
Godlewski G,Jourdan T,Szanda G,Tam J,Cinar R,Harvey-White J,Liu J,Mukhopadhyay B,Pacher P,Ming Mo F,Osei-Hyiaman D,Kunos G

Developmental expression of GPR3 in rodent cerebellar granule neurons is associated with cell survival and protects neurons from various apoptotic stimuli.
Neurobiology of disease68215-27(2014 Aug)
Tanaka S,Miyagi T,Dohi E,Seki T,Hide I,Sotomaru Y,Saeki Y,Antonio Chiocca E,Matsumoto M,Sakai N

Immunolocalization and expression pattern of Gpr3 in the ovary and its effect on proliferation of ovarian granulosa cells in pigs.
The Journal of reproduction and development58(4)410-9(2012)
Zhang B,Wei Q,Shi S,Dong F,Shi F,Xu Y

The G protein coupled receptor 3 is involved in cAMP and cGMP signaling and maintenance of meiotic arrest in porcine oocytes.
PloS one7(6)e38807(2012)
Yang CR,Wei Y,Qi ST,Chen L,Zhang QH,Ma JY,Luo YB,Wang YP,Hou Y,Schatten H,Liu ZH,Sun QY

The G-protein-coupled receptors GPR3 and GPR12 are involved in cAMP signaling and maintenance of meiotic arrest in rodent oocytes.
Developmental biology287(2)249-61(2005 Nov)
Hinckley M,Vaccari S,Horner K,Chen R,Conti M

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Gpr3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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