Hspa4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspa4
Entrez Gene ID	266759
Full Name	heat shock protein family A member 4
Synonyms	Hsp110,Hsp70,irp94
General protein information	Preferred Names heat shock protein family A member 4 Names heat shock 70 kDa protein 4 heat shock protein 4 ischemia responsive 94 kDa protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22
Summary	heat shock protein; may have an important role following forebrain ischemia and may allow astrocytes to protect neurons [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_153629.1	NP_705893.1	heat shock 70 kDa protein 4

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	HSPA4	XP_414655.2
Caenorhabditis elegans (roundworm)	C30C11.4	NP_498868.1
Saccharomyces cerevisiae (baker's yeast)	SSE1	NP_015219.1
Eremothecium gossypii	AGOS_AGR212W	NP_986878.1
Magnaporthe oryzae (rice blast fungus)	MGG_06065	XP_003711919.1
Arabidopsis thaliana (thale cress)	AT1G79920	NP_850984.4
Oryza sativa (rice)	Os05g0181000	NP_001054812.1
Homo sapiens (human)	HSPA4	NP_002145.3
Mus musculus (house mouse)	Hspa4	NP_032326.3
Danio rerio (zebrafish)	hspa4a	NP_999881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010331	XP_315285.3
Kluyveromyces lactis	KLLA0E24597g	XP_455059.1
Rattus norvegicus (Norway rat)	Hspa4	NP_705893.1
	HSPA4	XP_001106968.1
Danio rerio (zebrafish)	hspa4b	NP_956151.1
Drosophila melanogaster (fruit fly)	Hsc70Cb	NP_001189103.1
Schizosaccharomyces pombe (fission yeast)	pss1	XP_001713060.1
Xenopus tropicalis (tropical clawed frog)	hspa4	NP_989252.1
Pan troglodytes (chimpanzee)	HSPA4	XP_517930.2
Canis lupus familiaris (dog)	HSPA4	NP_001041481.1
Bos taurus (cattle)	HSPA4	NP_001107664.1
Neurospora crassa	NCU05269	XP_961759.2
Arabidopsis thaliana (thale cress)	HSP91	NP_178111.1
Oryza sativa (rice)	Os01g0180800	NP_001042210.1

Gene Ontology
Bibliography

Related articles in PubMed

Heat adaptation from regular hot water immersion decreases proinflammatory responses, HSP70 expression, and physical heat stress.
Yang FL, Lee CC, Subeq YM, Lee CJ, Ke CY, Lee RP
Journal of thermal biology6995-103(2017 Oct)

Temporal regulation of extracellular signal-regulated kinase 1/2 phosphorylation, heat shock protein 70 and activating transcription factor 3 during prostaglandin F-induced luteal regression in pseudopregnant rats following heat stress.
Bai WJ, Jin PJ, Kuang MQ, Wei QW, Shi FX, Davis JS, Mao DG
Reproduction, fertility, and development29(6)1184-1193(2017 Jun)

Vitamin A Oral Supplementation Induces Oxidative Stress and Suppresses IL-10 and HSP70 in Skeletal Muscle of Trained Rats.
Petiz LL, Girardi CS, Bortolin RC, Kunzler A, Gasparotto J, Rabelo TK, Matt? C, Moreira JC, Gelain DP
Nutrients9(4)(2017 Apr)

Ste20-like kinase, SLK, activates the heat shock factor 1 - Hsp70 pathway.
Cybulsky AV, Guillemette J, Papillon J
Biochimica et biophysica acta1863(9)2147-55(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that vitamin A, when used as an unnecessary dietary supplement, decreases skeletal muscle expression of anti-inflammatory mediators interleukin-10 and heat shock protein 70, important factors for positive exercise adaptations and tissue damage prevention.
Title: Vitamin A Oral Supplementation Induces Oxidative Stress and Suppresses IL-10 and HSP70 in Skeletal Muscle of Trained Rats.

increased expression/activity of SLK activates the HSF1-Hsp70 pathway.
Title: Ste20-like kinase, SLK, activates the heat shock factor 1 - Hsp70 pathway.

Renal Hsp70 expression is decreased in spontaneously hypertensive rats before the development of hypertension.
Title: Changes in renal WT-1 expression preceding hypertension development.

These results suggest that electromagnetic preconditioning ameliorates calcium homeostasis through activating HSP70, thereby producing the cardioprotective effect and reduction in hypoxic cardiomyocytes damage.
Title: EMF protects cardiomyocytes against hypoxia-induced injury via heat shock protein 70 activation.

Expression of HSP70 reduced pulmonary arterial pressure and pulmonary vascular remodeling in neonatal rats with hypoxic pulmonary hypertension.
Title: [Effect of heat shock protein 70 on pulmonary vascular remodeling in neonatal rats with hypoxic pulmonary hypertension].

The following Hspa4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspa4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03113	NM_153629.1 Latest version!	Rattus norvegicus heat shock protein family A (Hsp70) member 4 (Hspa4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspa4 ( NM_153629.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_705893.1 Rattus norvegicus heat shock protein family A (Hsp70) member 4 (Hspa4), mRNA.

Price & Availability↑

CloneID	ORa03113
Clone ID Related Accession (Same CDS sequence)	NM_153629.1
Accession Version	NM_153629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Rattus norvegicus(Norway rat)
Product	heat shock 70 kDa protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF077354.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF077354.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGGTGG TGGGCATAGA CCTGGGCTTT CAGAGCTGCT ATGTCGCTGT GGCCCGCGCC 
GGCGGCATTG AGACGATCGC TAATGAGTAT AGCGACCGCT GCACGCCGGC TTGTGTTTCT 
TTCGGTCCTA AGAATCGTTC AGTTGGAGCA GCAGCAAAGA GCCAGGTAAT TTCCAATGCC 
AAGAACACAG TTCAAGGGTT TAAAAGATTT CATGGGCGTG CATTCTCAGA TCCATTTGTG 
GAAGCAGAAA AATCTAACCT TGCATATGAT ATTGTACAGT TGCCCACTGG ATTAACGGGC 
ATAAAGGTTA CATATATGGA AGAAGAGAGG AATTTTACCA CTGAACAAGT GACTGCCATG 
CTCTTGTCAA AACTGAAGGA GACAGCAGAA AGTGTTCTTA AGAAGCCTGT TGTAGACTGT 
GTTGTTTCGG TTCCTAGTTT CTACACTGAT GCAGAACGAC GGTCAGTGAT GGATGCCACA 
CAGATTGCTG GTCTCAATTG CTTGCGGTTA ATGAATGAAA CAACTGCAGT TGCTCTTGCT 
TATGGAATCT ATAAACAAGA CCTTCCTGCC TTAGAAGAAA AACCAAGAAA TGTAGTTTTT 
GTAGATATGG GACATTCTGC CTATCAAGTT TCTGTATGTG CATTTAATAG AGGAAAACTG 
AAAGTTTTGG CCACAGCTTT TGATACAACA TTGGGAGGCA GAAAATTTGA TGAGGTGTTG 
GTAAATCACT TCTGTGAAGA ATTTGGAAAG AAATACAAGC TAGACATAAA ATCTAAAGTT 
CGTGCTTTAT TGCGACTCTC TCAAGAGTGT GAGAAACTCA AGAAATTAAT GAGTGCGAAT 
GCTTCAGACC TCCCTTTGAG TATTGAATGC TTTATGAACG ATATTGATGT ATCGGGAACC 
ATGAATAGAG GCAAATTTTT GGAGATGTGT GATGATCTCT TAGCAAGAGT GGAGCCGCCA 
CTTCGTAGCA TCTTGGACCA ATCCAAGTTA AAGAAAGAAG ATATTTATGC AGTGGAGATA 
GTTGGTGGAG CCACACGGAT CCCTGCCGTG AAAGAGAAGA TCAGCAAGTT CTTTGGTAAA 
GAACTCAGCA CAACATTAAA TGCGGATGAA GCAGTCACTC GAGGCTGTGC TCTGCAGTGT 
GCAATCTTAT CACCTGCTTT CAAAGTCAGA GAATTTTCTA TCACTGATGT GGTACCATAT 
CCAATATCTT TGAGGTGGAA TTCTCCAGCT GAAGAGGGGT CAAGTGACTG TGAAGTCTTC 
CCCAAAAACC ATGCTGCTCC TTTCTCCAAA GTCCTCACCT TTTATAGAAA GGAACCGTTC 
ACTCTGGAGG CCTACTATAG TTCTCCTCAG GATTTGCCCT ATCCAGATCC TGCTATAGCT 
CAGTTTTCAG TTCAGAAAGT CACTCCACAG TCTGATGGCT CCAGCTCAAA AGTGAAAGTC 
AAGGTCCGGG TAAATGTGCA TGGCATTTTC AGTGTGTCCA GTGCGGCCTT AGTAGAGGTG 
CACAAGTCCG AGGAGAGCGA GGAGCCAATG GAGACTGATC AGAATGCAAA GGAAGAAGAG 
AAGATGCAGG TGGACCAGGA AGAACCACAT ACTGAAGAGC AACAGCCACA GACACCAGCA 
GAAAACAAGG CGGAGTCTGA GGAGATGGAG ACCTCTCAAG CTGGATCAAA AGATAAAAAG 
ATGGACCAAC CACCTCAAGC AAAGAAGGCA AAAGTGAAGA CCAGCACCGT GGACCTGCCC 
ATTGAGAGCC AGCTGCTGTG GCAGCTGGAC CGGGAAATGC TCGGCCTGTA CACAGAGAAT 
GAGGGTAAGA TGATCATGCA GGATAAACTG GAGAAGGAAC GGAATGACGC CAAAAATGCG 
GTGGAAGAGT ATGTGTATGA GATGAGAGAC AAACTTAGTG GTGAATATGA GAAGTTTGTG 
AGTGAAGATG ATCGTAATAA TTTTACTTTG AAATTAGAGG ATACAGAAAA CTGGCTGTAT 
GAAGATGGAG AAGATCAACC AAAGCAAGTT TATGTAGACA AATTGGCTGA GTTAAGAACT 
TTAGGTCAAC CTATTAAGAC ACGGTTCCAG GAATCTGAAG AAAGACCAAA ATTATTTGAA 
GAATTAGGGA AGCAAATCCA ACAGTATATG AAAGTGATCA GCTCTTTCAA AAACAAGGAG 
GACCAGTATG AACACTTGGA TGCTGCTGAC ATGACAAAGG TAGAGAAAAG CACCAATGAG 
GCGATGGAGT GGATGAATAG TAAGCTGAAC CTGCAGAACA AACAGAGCCT GACGGCGGAT 
CCAGTTGTCA AGACAAAAGA GATTGAAGCT AAAATTAAGG AGCTGACAAA TATTTGCAGT 
CCTATAATTT CAAAGCCCAA ACCCAAAGTG GAACCCCCTA AGGAGGAACC AAAACATGCT 
GAGCAGAATG GACCAGTGGA TGGCCAAGGA GACAACCCAG GCACCCAAGC TGCTGAGCAC 
GGTGCAGACA CAGCTGTGCC TTCTGATGGT GACAAGAAGC TTCCTGAGAT GGACATTGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705893.1
CDS	226..2748
Translation	MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACVSFGPKNRSVGAAAKSQVISNAKNTVQGFKRFHGRAFSDPFVEAEKSNLAYDIVQLPTGLTGIKVTYMEEERNFTTEQVTAMLLSKLKETAESVLKKPVVDCVVSVPSFYTDAERRSVMDATQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVFVDMGHSAYQVSVCAFNRGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKVRALLRLSQECEKLKKLMSANASDLPLSIECFMNDIDVSGTMNRGKFLEMCDDLLARVEPPLRSILDQSKLKKEDIYAVEIVGGATRIPAVKEKISKFFGKELSTTLNADEAVTRGCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGSSDCEVFPKNHAAPFSKVLTFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIFSVSSAALVEVHKSEESEEPMETDQNAKEEEKMQVDQEEPHTEEQQPQTPAENKAESEEMETSQAGSKDKKMDQPPQAKKAKVKTSTVDLPIESQLLWQLDREMLGLYTENEGKMIMQDKLEKERNDAKNAVEEYVYEMRDKLSGEYEKFVSEDDRNNFTLKLEDTENWLYEDGEDQPKQVYVDKLAELRTLGQPIKTRFQESEERPKLFEELGKQIQQYMKVISSFKNKEDQYEHLDAADMTKVEKSTNEAMEWMNSKLNLQNKQSLTADPVVKTKEIEAKIKELTNICSPIISKPKPKVEPPKEEPKHAEQNGPVDGQGDNPGTQAAEHGADTAVPSDGDKKLPEMDID

Target ORF information:

RefSeq Version	NM_153629.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein family A (Hsp70) member 4 (Hspa4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153629.1

ORF Insert Sequence:

ATGTCGGTGG TGGGCATAGA CCTGGGCTTT CAGAGCTGCT ATGTCGCTGT GGCCCGCGCC 
GGCGGCATTG AGACGATCGC TAATGAGTAT AGCGACCGCT GCACGCCGGC TTGTGTTTCT 
TTCGGTCCTA AGAATCGTTC AGTTGGAGCA GCAGCAAAGA GCCAGGTAAT TTCCAATGCC 
AAGAACACAG TTCAAGGGTT TAAAAGATTT CATGGGCGTG CATTCTCAGA TCCATTTGTG 
GAAGCAGAAA AATCTAACCT TGCATATGAT ATTGTACAGT TGCCCACTGG ATTAACGGGC 
ATAAAGGTTA CATATATGGA AGAAGAGAGG AATTTTACCA CTGAACAAGT GACTGCCATG 
CTCTTGTCAA AACTGAAGGA GACAGCAGAA AGTGTTCTTA AGAAGCCTGT TGTAGACTGT 
GTTGTTTCGG TTCCTAGTTT CTACACTGAT GCAGAACGAC GGTCAGTGAT GGATGCCACA 
CAGATTGCTG GTCTCAATTG CTTGCGGTTA ATGAATGAAA CAACTGCAGT TGCTCTTGCT 
TATGGAATCT ATAAACAAGA CCTTCCTGCC TTAGAAGAAA AACCAAGAAA TGTAGTTTTT 
GTAGATATGG GACATTCTGC CTATCAAGTT TCTGTATGTG CATTTAATAG AGGAAAACTG 
AAAGTTTTGG CCACAGCTTT TGATACAACA TTGGGAGGCA GAAAATTTGA TGAGGTGTTG 
GTAAATCACT TCTGTGAAGA ATTTGGAAAG AAATACAAGC TAGACATAAA ATCTAAAGTT 
CGTGCTTTAT TGCGACTCTC TCAAGAGTGT GAGAAACTCA AGAAATTAAT GAGTGCGAAT 
GCTTCAGACC TCCCTTTGAG TATTGAATGC TTTATGAACG ATATTGATGT ATCGGGAACC 
ATGAATAGAG GCAAATTTTT GGAGATGTGT GATGATCTCT TAGCAAGAGT GGAGCCGCCA 
CTTCGTAGCA TCTTGGACCA ATCCAAGTTA AAGAAAGAAG ATATTTATGC AGTGGAGATA 
GTTGGTGGAG CCACACGGAT CCCTGCCGTG AAAGAGAAGA TCAGCAAGTT CTTTGGTAAA 
GAACTCAGCA CAACATTAAA TGCGGATGAA GCAGTCACTC GAGGCTGTGC TCTGCAGTGT 
GCAATCTTAT CACCTGCTTT CAAAGTCAGA GAATTTTCTA TCACTGATGT GGTACCATAT 
CCAATATCTT TGAGGTGGAA TTCTCCAGCT GAAGAGGGGT CAAGTGACTG TGAAGTCTTC 
CCCAAAAACC ATGCTGCTCC TTTCTCCAAA GTCCTCACCT TTTATAGAAA GGAACCGTTC 
ACTCTGGAGG CCTACTATAG TTCTCCTCAG GATTTGCCCT ATCCAGATCC TGCTATAGCT 
CAGTTTTCAG TTCAGAAAGT CACTCCACAG TCTGATGGCT CCAGCTCAAA AGTGAAAGTC 
AAGGTCCGGG TAAATGTGCA TGGCATTTTC AGTGTGTCCA GTGCGGCCTT AGTAGAGGTG 
CACAAGTCCG AGGAGAGCGA GGAGCCAATG GAGACTGATC AGAATGCAAA GGAAGAAGAG 
AAGATGCAGG TGGACCAGGA AGAACCACAT ACTGAAGAGC AACAGCCACA GACACCAGCA 
GAAAACAAGG CGGAGTCTGA GGAGATGGAG ACCTCTCAAG CTGGATCAAA AGATAAAAAG 
ATGGACCAAC CACCTCAAGC AAAGAAGGCA AAAGTGAAGA CCAGCACCGT GGACCTGCCC 
ATTGAGAGCC AGCTGCTGTG GCAGCTGGAC CGGGAAATGC TCGGCCTGTA CACAGAGAAT 
GAGGGTAAGA TGATCATGCA GGATAAACTG GAGAAGGAAC GGAATGACGC CAAAAATGCG 
GTGGAAGAGT ATGTGTATGA GATGAGAGAC AAACTTAGTG GTGAATATGA GAAGTTTGTG 
AGTGAAGATG ATCGTAATAA TTTTACTTTG AAATTAGAGG ATACAGAAAA CTGGCTGTAT 
GAAGATGGAG AAGATCAACC AAAGCAAGTT TATGTAGACA AATTGGCTGA GTTAAGAACT 
TTAGGTCAAC CTATTAAGAC ACGGTTCCAG GAATCTGAAG AAAGACCAAA ATTATTTGAA 
GAATTAGGGA AGCAAATCCA ACAGTATATG AAAGTGATCA GCTCTTTCAA AAACAAGGAG 
GACCAGTATG AACACTTGGA TGCTGCTGAC ATGACAAAGG TAGAGAAAAG CACCAATGAG 
GCGATGGAGT GGATGAATAG TAAGCTGAAC CTGCAGAACA AACAGAGCCT GACGGCGGAT 
CCAGTTGTCA AGACAAAAGA GATTGAAGCT AAAATTAAGG AGCTGACAAA TATTTGCAGT 
CCTATAATTT CAAAGCCCAA ACCCAAAGTG GAACCCCCTA AGGAGGAACC AAAACATGCT 
GAGCAGAATG GACCAGTGGA TGGCCAAGGA GACAACCCAG GCACCCAAGC TGCTGAGCAC 
GGTGCAGACA CAGCTGTGCC TTCTGATGGT GACAAGAAGC TTCCTGAGAT GGACATTGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heat adaptation from regular hot water immersion decreases proinflammatory responses, HSP70 expression, and physical heat stress.
Journal of thermal biology6995-103(2017 Oct)
Yang FL,Lee CC,Subeq YM,Lee CJ,Ke CY,Lee RP

Temporal regulation of extracellular signal-regulated kinase 1/2 phosphorylation, heat shock protein 70 and activating transcription factor 3 during prostaglandin F-induced luteal regression in pseudopregnant rats following heat stress.
Reproduction, fertility, and development29(6)1184-1193(2017 Jun)
Bai WJ,Jin PJ,Kuang MQ,Wei QW,Shi FX,Davis JS,Mao DG

Vitamin A Oral Supplementation Induces Oxidative Stress and Suppresses IL-10 and HSP70 in Skeletal Muscle of Trained Rats.
Nutrients9(4)(2017 Apr)
Petiz LL,Girardi CS,Bortolin RC,Kunzler A,Gasparotto J,Rabelo TK,Matt? C,Moreira JC,Gelain DP

Ste20-like kinase, SLK, activates the heat shock factor 1 - Hsp70 pathway.
Biochimica et biophysica acta1863(9)2147-55(2016 09)
Cybulsky AV,Guillemette J,Papillon J

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Hspa4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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