Gjc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gjc1
Entrez Gene ID	266706
Full Name	gap junction protein, gamma 1
Synonyms	Cx45,Gja7
General protein information	Preferred Names gap junction protein, gamma 1 Names gap junction gamma-1 protein connexin-45 gap junction alpha-7 protein gap junction channel protein connexin 45 gap junction membrane channel protein alpha 7
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	component of gap junctions; involved in intercellular communication in arterial smooth muscle [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597050.1	XP_017452539.1	gap junction gamma-1 protein isoform X1
NM_001085381.1	NP_001078850.1	gap junction gamma-1 protein
XM_017597049.1	XP_017452538.1	gap junction gamma-1 protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GJC1	XP_511557.1
	GJC1	XP_001114752.1
Mus musculus (house mouse)	Gjc1	NP_032148.1
Canis lupus familiaris (dog)	GJC1	NP_001018648.1
Rattus norvegicus (Norway rat)	Gjc1	NP_001078850.1
Danio rerio (zebrafish)	LOC564307	XP_005163904.1
Xenopus tropicalis (tropical clawed frog)	gjc1	XP_002935609.1
Homo sapiens (human)	GJC1	NP_001073852.1
Gallus gallus (chicken)	GJC1	NP_990834.1
Bos taurus (cattle)	GJC1	NP_001039541.1

Gene Ontology
Bibliography

Related articles in PubMed

Cx36, Cx43 and Cx45 in mouse and rat cerebellar cortex: species-specific expression, compensation in Cx36 null mice and co-localization in neurons vs. glia.
Nagy JI, Rash JE
The European journal of neuroscience46(2)1790-1804(2017 Jul)

Rapid upregulation of the hippocampal connexins 36 and 45 mRNA levels during memory consolidation.
Beheshti S, Zeinali R, Esmaeili A
Behavioural brain research32085-90(2017 03)

Gap junction proteins in the light-damaged albino rat.
Guo CX, Tran H, Green CR, Danesh-Meyer HV, Acosta ML
Molecular vision20670-82(2014)

De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy.
Cea LA, Cisterna BA, Puebla C, Frank M, Figueroa XF, Cardozo C, Willecke K, Latorre R, S?ez JC
Proceedings of the National Academy of Sciences of the United States of America110(40)16229-34(2013 Oct)

Connexin45 provides optimal atrioventricular nodal conduction in the adult mouse heart.
Frank M, Wirth A, Andri? RP, Kreuzberg MM, Dobrowolski R, Seifert G, Offermanns S, Nickenig G, Willecke K, Schrickel JW
Circulation research111(12)1528-38(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of the present study revealed that during memory consolidation in a passive avoidance paradigm, a rapid upregulation of Cx45 mRNAs level occurs in the hippocampus, which declines to the control level by 24 h after training.
Title: Rapid upregulation of the hippocampal connexins 36 and 45 mRNA levels during memory consolidation.

Changes in connexins, including Cx36, Cx43, and Cx45, may contribute to the damage process.
Title: Gap junction proteins in the light-damaged albino rat.

The absence of Cx43/Cx45 hemichannels in skeltal muscles cells greatly attenuates the inflammasome activation and muscle atrophy.
Title: De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy.

Findings demonstrate that overexpression of connexin 45 and subsequent formation of heteromeric connexin 45/connexin 43 gap junction channels in HCN4 expressing MSCs can improve their function as cardiac biological pacemakers in vitro.
Title: Overexpression of connexin 45 in rat mesenchymal stem cells improves the function as cardiac biological pacemakers.

Report regulation of connexin 45 gene expression during skeletal muscle regeneration in the adult rat.
Title: Regulation of connexin gene expression during skeletal muscle regeneration in the adult rat.

The following Gjc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gjc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13153	NM_001085381.1 Latest version!	Rattus norvegicus gap junction protein, gamma 1 (Gjc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa57201	XM_017597050.1 Latest version!	Rattus norvegicus gap junction protein, gamma 1 (Gjc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa57201	XM_017597049.1 Latest version!	Rattus norvegicus gap junction protein, gamma 1 (Gjc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gjc1 ( NM_001085381.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001078850.1 Rattus norvegicus gap junction protein, gamma 1 (Gjc1), mRNA.

Price & Availability↑

CloneID	ORa13153
Clone ID Related Accession (Same CDS sequence)	NM_001085381.1
Accession Version	NM_001085381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	gap junction gamma-1 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ871218.1. On Apr 27, 2007 this sequence version replaced XM_001081521.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGTTGGA GCTTCCTGAC TCGCCTGCTA GAGGAGATAC ACAACCATTC GACGTTTGTA 
GGGAAGATCT GGCTCACTGT GCTGATTGTC TTTCGAATTG TCCTAACTGC GGTAGGAGGA 
GAGTCCATCT ACTATGATGA GCAAAGCAAA TTTGTGTGCA ACACAGAGCA GCCGGGCTGT 
GAGAACGTCT GCTATGATGC CTTTGCCCCG CTCTCCCACG TGCGCTTCTG GGTATTCCAG 
ATCATCCTGG TTGCTACTCC CTCGGTGATG TACCTGGGAT ATGCTATTCA CAAGATTGCC 
AAAATGGAGC ACGGCGAGGC AGACAAGAAG GCAGCTCGGA GCAAACCCTA TGCCATGCGT 
TGGAAGCAGC ACCGGGCTCT GGAAGAAACG GAAGAGGACC ATGAAGAGGA TCCTATGATG 
TACCCAGAGA TGGAGTTAGA AAGCGAGAAA GAAAATAAAG AGCAGAGCCA ACCAAAACCC 
AAGCATGATG GCCGCCGACG CATTCGTGAG GATGGGCTCA TGAAAATCTA TGTGTTGCAG 
CTGCTAGCCA GGACTGTGTT TGAGGTGGGC TTTCTCATAG GGCAGTATTT CCTGTATGGC 
TTCCAAGTAC ACCCATTTTA TGTGTGCAGC AGACTTCCTT GTCCTCATAA GATAGACTGC 
TTTATTTCTA GACCCACTGA AAAGACCATC TTCCTTCTGA TAATGTATGG TGTCACAGGC 
CTCTGCCTAT TGCTTAACAT TTGGGAGATG CTTCATCTAG GGTTTGGGAC CATTCGAGAC 
TCACTAAACA GTAAAAGGAG GGAACTTGAT GATCCGGGTG CTTATAATTA TCCTTTCACT 
TGGAATACAC CATCTGCTCC CCCAGGCTAT AACATTGCTG TCAAACCAGA TCAAATCCAG 
TACACTGAGT TGTCCAATGC TAAGATTGCC TACAAGCAGA ACAAAGCCAA TATCGCCCAG 
GAGCAGCAGT ATGGCAGCCA CGAGGAGCAC CTCCCGGCCG ATCTGGAGAC CCTGCAGCGG 
GAGATCAGAA TGGCCCAGGA ACGCTTGGAT CTAGCAATCC AGGCCTACCA TCACCAGAAC 
AACCCCCATG GTCCTCGGGA AAAGAAGGCC AAAGTGGGGT CCAAATCTGG GTCCAACAAA 
AGCAGTATTA GTAGCAAATC AGGAGATGGG AAGACCTCCG TCTGGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078850.1
CDS	409..1599
Translation	MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAPLSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDHEEDPMMYPEMELESEKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCSRLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELDDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANIAQEQQYGSHEEHLPADLETLQREIRMAQERLDLAIQAYHHQNNPHGPREKKAKVGSKSGSNKSSISSKSGDGKTSVWI

Target ORF information:

RefSeq Version	NM_001085381.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gap junction protein, gamma 1 (Gjc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085381.1

ORF Insert Sequence:

ATGAGTTGGA GCTTCCTGAC TCGCCTGCTA GAGGAGATAC ACAACCATTC GACGTTTGTA 
GGGAAGATCT GGCTCACTGT GCTGATTGTC TTTCGAATTG TCCTAACTGC GGTAGGAGGA 
GAGTCCATCT ACTATGATGA GCAAAGCAAA TTTGTGTGCA ACACAGAGCA GCCGGGCTGT 
GAGAACGTCT GCTATGATGC CTTTGCCCCG CTCTCCCACG TGCGCTTCTG GGTATTCCAG 
ATCATCCTGG TTGCTACTCC CTCGGTGATG TACCTGGGAT ATGCTATTCA CAAGATTGCC 
AAAATGGAGC ACGGCGAGGC AGACAAGAAG GCAGCTCGGA GCAAACCCTA TGCCATGCGT 
TGGAAGCAGC ACCGGGCTCT GGAAGAAACG GAAGAGGACC ATGAAGAGGA TCCTATGATG 
TACCCAGAGA TGGAGTTAGA AAGCGAGAAA GAAAATAAAG AGCAGAGCCA ACCAAAACCC 
AAGCATGATG GCCGCCGACG CATTCGTGAG GATGGGCTCA TGAAAATCTA TGTGTTGCAG 
CTGCTAGCCA GGACTGTGTT TGAGGTGGGC TTTCTCATAG GGCAGTATTT CCTGTATGGC 
TTCCAAGTAC ACCCATTTTA TGTGTGCAGC AGACTTCCTT GTCCTCATAA GATAGACTGC 
TTTATTTCTA GACCCACTGA AAAGACCATC TTCCTTCTGA TAATGTATGG TGTCACAGGC 
CTCTGCCTAT TGCTTAACAT TTGGGAGATG CTTCATCTAG GGTTTGGGAC CATTCGAGAC 
TCACTAAACA GTAAAAGGAG GGAACTTGAT GATCCGGGTG CTTATAATTA TCCTTTCACT 
TGGAATACAC CATCTGCTCC CCCAGGCTAT AACATTGCTG TCAAACCAGA TCAAATCCAG 
TACACTGAGT TGTCCAATGC TAAGATTGCC TACAAGCAGA ACAAAGCCAA TATCGCCCAG 
GAGCAGCAGT ATGGCAGCCA CGAGGAGCAC CTCCCGGCCG ATCTGGAGAC CCTGCAGCGG 
GAGATCAGAA TGGCCCAGGA ACGCTTGGAT CTAGCAATCC AGGCCTACCA TCACCAGAAC 
AACCCCCATG GTCCTCGGGA AAAGAAGGCC AAAGTGGGGT CCAAATCTGG GTCCAACAAA 
AGCAGTATTA GTAGCAAATC AGGAGATGGG AAGACCTCCG TCTGGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjc1 ( XM_017597050.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452539.1 Rattus norvegicus gap junction protein, gamma 1 (Gjc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57201
Clone ID Related Accession (Same CDS sequence)	XM_017597050.1 , XM_017597049.1
Accession Version	XM_017597050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gap junction gamma-1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTGGA GCTTCCTGAC TCGCCTGCTA GAGGAGATAC ACAACCATTC GACGTTTGTA 
GGGAAGATCT GGCTCACTGT GCTGATTGTC TTTCGAATTG TCCTAACTGC GGTAGGAGGA 
GAGTCCATCT ACTATGATGA GCAAAGCAAA TTTGTGTGCA ACACAGAGCA GCCGGGCTGT 
GAGAACGTCT GCTATGATGC CTTTGCCCCG CTCTCCCACG TGCGCTTCTG GGTATTCCAG 
ATCATCCTGG TTGCTACTCC CTCTGTGATG TACCTGGGAT ATGCTATTCA CAAGATTGCC 
AAAATGGAGC ACGGCGAGGC AGACAAGAAG GCAGCTCGGA GCAAACCCTA TGCCATGCGT 
TGGAAGCAGC ACCGGGCTCT GGAAGAAACG GAAGAGGACC ATGAAGAGGA TCCTATGATG 
TACCCAGAGA TGGAGTTAGA AAGCGAGAAA GAAAATAAAG AGCAGAGCCA ACCAAAACCC 
AAGCATGATG GCCGCCGACG CATTCGTGAG GATGGGCTCA TGAAAATCTA TGTGTTGCAG 
CTGCTAGCCA GGACTGTGTT TGAGGTGGGC TTTCTCATAG GGCAGTATTT CCTGTATGGC 
TTCCAAGTAC ACCCATTTTA TGTGTGCAGC AGACTTCCTT GTCCTCATAA GATAGACTGC 
TTTATTTCTA GACCCACTGA AAAGACCATC TTCCTTCTGA TAATGTATGG TGTCACAGGC 
CTCTGCCTAT TGCTTAACAT TTGGGAGATG CTTCATCTAG GGTTTGGGAC CATTCGAGAC 
TCACTAAACA GTAAAAGGAG GGAACTTGAT GATCCGGGTG CTTATAATTA TCCTTTCACT 
TGGAATACAC CATCTGCTCC CCCAGGCTAT AACATTGCTG TCAAACCAGA TCAAATCCAG 
TACACTGAGT TGTCCAATGC TAAGATTGCC TACAAGCAGA ACAAAGCCAA TATCGCCCAG 
GAGCAGCAGT ACGGCAGCCA CGAGGAGCAC CTCCCGGCCG ATCTGGAGAC CCTGCAGCGG 
GAGATCAGAA TGGCCCAGGA ACGCTTGGAT CTAGCAATCC AGGCCTACCA TCACCAGAAC 
AACCCCCATG GTCCTCGGGA AAAGAAGGCC AAAGTGGGGT CCAAATCTGG GTCCAACAAA 
AGCAGTATTA GTAGCAAATC AGGAGATGGG AAGACCTCCG TCTGGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452539.1
CDS	731..1921
Translation	MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAPLSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDHEEDPMMYPEMELESEKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCSRLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELDDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANIAQEQQYGSHEEHLPADLETLQREIRMAQERLDLAIQAYHHQNNPHGPREKKAKVGSKSGSNKSSISSKSGDGKTSVWI

Target ORF information:

RefSeq Version	XM_017597050.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gap junction protein, gamma 1 (Gjc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597050.1

ORF Insert Sequence:

ATGAGTTGGA GCTTCCTGAC TCGCCTGCTA GAGGAGATAC ACAACCATTC GACGTTTGTA 
GGGAAGATCT GGCTCACTGT GCTGATTGTC TTTCGAATTG TCCTAACTGC GGTAGGAGGA 
GAGTCCATCT ACTATGATGA GCAAAGCAAA TTTGTGTGCA ACACAGAGCA GCCGGGCTGT 
GAGAACGTCT GCTATGATGC CTTTGCCCCG CTCTCCCACG TGCGCTTCTG GGTATTCCAG 
ATCATCCTGG TTGCTACTCC CTCTGTGATG TACCTGGGAT ATGCTATTCA CAAGATTGCC 
AAAATGGAGC ACGGCGAGGC AGACAAGAAG GCAGCTCGGA GCAAACCCTA TGCCATGCGT 
TGGAAGCAGC ACCGGGCTCT GGAAGAAACG GAAGAGGACC ATGAAGAGGA TCCTATGATG 
TACCCAGAGA TGGAGTTAGA AAGCGAGAAA GAAAATAAAG AGCAGAGCCA ACCAAAACCC 
AAGCATGATG GCCGCCGACG CATTCGTGAG GATGGGCTCA TGAAAATCTA TGTGTTGCAG 
CTGCTAGCCA GGACTGTGTT TGAGGTGGGC TTTCTCATAG GGCAGTATTT CCTGTATGGC 
TTCCAAGTAC ACCCATTTTA TGTGTGCAGC AGACTTCCTT GTCCTCATAA GATAGACTGC 
TTTATTTCTA GACCCACTGA AAAGACCATC TTCCTTCTGA TAATGTATGG TGTCACAGGC 
CTCTGCCTAT TGCTTAACAT TTGGGAGATG CTTCATCTAG GGTTTGGGAC CATTCGAGAC 
TCACTAAACA GTAAAAGGAG GGAACTTGAT GATCCGGGTG CTTATAATTA TCCTTTCACT 
TGGAATACAC CATCTGCTCC CCCAGGCTAT AACATTGCTG TCAAACCAGA TCAAATCCAG 
TACACTGAGT TGTCCAATGC TAAGATTGCC TACAAGCAGA ACAAAGCCAA TATCGCCCAG 
GAGCAGCAGT ACGGCAGCCA CGAGGAGCAC CTCCCGGCCG ATCTGGAGAC CCTGCAGCGG 
GAGATCAGAA TGGCCCAGGA ACGCTTGGAT CTAGCAATCC AGGCCTACCA TCACCAGAAC 
AACCCCCATG GTCCTCGGGA AAAGAAGGCC AAAGTGGGGT CCAAATCTGG GTCCAACAAA 
AGCAGTATTA GTAGCAAATC AGGAGATGGG AAGACCTCCG TCTGGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjc1 ( XM_017597049.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452538.1 Rattus norvegicus gap junction protein, gamma 1 (Gjc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57201
Clone ID Related Accession (Same CDS sequence)	XM_017597050.1 , XM_017597049.1
Accession Version	XM_017597049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gap junction gamma-1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTGGA GCTTCCTGAC TCGCCTGCTA GAGGAGATAC ACAACCATTC GACGTTTGTA 
GGGAAGATCT GGCTCACTGT GCTGATTGTC TTTCGAATTG TCCTAACTGC GGTAGGAGGA 
GAGTCCATCT ACTATGATGA GCAAAGCAAA TTTGTGTGCA ACACAGAGCA GCCGGGCTGT 
GAGAACGTCT GCTATGATGC CTTTGCCCCG CTCTCCCACG TGCGCTTCTG GGTATTCCAG 
ATCATCCTGG TTGCTACTCC CTCTGTGATG TACCTGGGAT ATGCTATTCA CAAGATTGCC 
AAAATGGAGC ACGGCGAGGC AGACAAGAAG GCAGCTCGGA GCAAACCCTA TGCCATGCGT 
TGGAAGCAGC ACCGGGCTCT GGAAGAAACG GAAGAGGACC ATGAAGAGGA TCCTATGATG 
TACCCAGAGA TGGAGTTAGA AAGCGAGAAA GAAAATAAAG AGCAGAGCCA ACCAAAACCC 
AAGCATGATG GCCGCCGACG CATTCGTGAG GATGGGCTCA TGAAAATCTA TGTGTTGCAG 
CTGCTAGCCA GGACTGTGTT TGAGGTGGGC TTTCTCATAG GGCAGTATTT CCTGTATGGC 
TTCCAAGTAC ACCCATTTTA TGTGTGCAGC AGACTTCCTT GTCCTCATAA GATAGACTGC 
TTTATTTCTA GACCCACTGA AAAGACCATC TTCCTTCTGA TAATGTATGG TGTCACAGGC 
CTCTGCCTAT TGCTTAACAT TTGGGAGATG CTTCATCTAG GGTTTGGGAC CATTCGAGAC 
TCACTAAACA GTAAAAGGAG GGAACTTGAT GATCCGGGTG CTTATAATTA TCCTTTCACT 
TGGAATACAC CATCTGCTCC CCCAGGCTAT AACATTGCTG TCAAACCAGA TCAAATCCAG 
TACACTGAGT TGTCCAATGC TAAGATTGCC TACAAGCAGA ACAAAGCCAA TATCGCCCAG 
GAGCAGCAGT ACGGCAGCCA CGAGGAGCAC CTCCCGGCCG ATCTGGAGAC CCTGCAGCGG 
GAGATCAGAA TGGCCCAGGA ACGCTTGGAT CTAGCAATCC AGGCCTACCA TCACCAGAAC 
AACCCCCATG GTCCTCGGGA AAAGAAGGCC AAAGTGGGGT CCAAATCTGG GTCCAACAAA 
AGCAGTATTA GTAGCAAATC AGGAGATGGG AAGACCTCCG TCTGGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452538.1
CDS	433..1623
Translation	MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAPLSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDHEEDPMMYPEMELESEKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCSRLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELDDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANIAQEQQYGSHEEHLPADLETLQREIRMAQERLDLAIQAYHHQNNPHGPREKKAKVGSKSGSNKSSISSKSGDGKTSVWI

Target ORF information:

RefSeq Version	XM_017597049.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gap junction protein, gamma 1 (Gjc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597049.1

ORF Insert Sequence:

ATGAGTTGGA GCTTCCTGAC TCGCCTGCTA GAGGAGATAC ACAACCATTC GACGTTTGTA 
GGGAAGATCT GGCTCACTGT GCTGATTGTC TTTCGAATTG TCCTAACTGC GGTAGGAGGA 
GAGTCCATCT ACTATGATGA GCAAAGCAAA TTTGTGTGCA ACACAGAGCA GCCGGGCTGT 
GAGAACGTCT GCTATGATGC CTTTGCCCCG CTCTCCCACG TGCGCTTCTG GGTATTCCAG 
ATCATCCTGG TTGCTACTCC CTCTGTGATG TACCTGGGAT ATGCTATTCA CAAGATTGCC 
AAAATGGAGC ACGGCGAGGC AGACAAGAAG GCAGCTCGGA GCAAACCCTA TGCCATGCGT 
TGGAAGCAGC ACCGGGCTCT GGAAGAAACG GAAGAGGACC ATGAAGAGGA TCCTATGATG 
TACCCAGAGA TGGAGTTAGA AAGCGAGAAA GAAAATAAAG AGCAGAGCCA ACCAAAACCC 
AAGCATGATG GCCGCCGACG CATTCGTGAG GATGGGCTCA TGAAAATCTA TGTGTTGCAG 
CTGCTAGCCA GGACTGTGTT TGAGGTGGGC TTTCTCATAG GGCAGTATTT CCTGTATGGC 
TTCCAAGTAC ACCCATTTTA TGTGTGCAGC AGACTTCCTT GTCCTCATAA GATAGACTGC 
TTTATTTCTA GACCCACTGA AAAGACCATC TTCCTTCTGA TAATGTATGG TGTCACAGGC 
CTCTGCCTAT TGCTTAACAT TTGGGAGATG CTTCATCTAG GGTTTGGGAC CATTCGAGAC 
TCACTAAACA GTAAAAGGAG GGAACTTGAT GATCCGGGTG CTTATAATTA TCCTTTCACT 
TGGAATACAC CATCTGCTCC CCCAGGCTAT AACATTGCTG TCAAACCAGA TCAAATCCAG 
TACACTGAGT TGTCCAATGC TAAGATTGCC TACAAGCAGA ACAAAGCCAA TATCGCCCAG 
GAGCAGCAGT ACGGCAGCCA CGAGGAGCAC CTCCCGGCCG ATCTGGAGAC CCTGCAGCGG 
GAGATCAGAA TGGCCCAGGA ACGCTTGGAT CTAGCAATCC AGGCCTACCA TCACCAGAAC 
AACCCCCATG GTCCTCGGGA AAAGAAGGCC AAAGTGGGGT CCAAATCTGG GTCCAACAAA 
AGCAGTATTA GTAGCAAATC AGGAGATGGG AAGACCTCCG TCTGGATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cx36, Cx43 and Cx45 in mouse and rat cerebellar cortex: species-specific expression, compensation in Cx36 null mice and co-localization in neurons vs. glia.
The European journal of neuroscience46(2)1790-1804(2017 Jul)
Nagy JI,Rash JE

Rapid upregulation of the hippocampal connexins 36 and 45 mRNA levels during memory consolidation.
Behavioural brain research32085-90(2017 03)
Beheshti S,Zeinali R,Esmaeili A

Gap junction proteins in the light-damaged albino rat.
Molecular vision20670-82(2014)
Guo CX,Tran H,Green CR,Danesh-Meyer HV,Acosta ML

De novo expression of connexin hemichannels in denervated fast skeletal muscles leads to atrophy.
Proceedings of the National Academy of Sciences of the United States of America110(40)16229-34(2013 Oct)
Cea LA,Cisterna BA,Puebla C,Frank M,Figueroa XF,Cardozo C,Willecke K,Latorre R,S?ez JC

Connexin45 provides optimal atrioventricular nodal conduction in the adult mouse heart.
Circulation research111(12)1528-38(2012 Dec)
Frank M,Wirth A,Andri? RP,Kreuzberg MM,Dobrowolski R,Seifert G,Offermanns S,Nickenig G,Willecke K,Schrickel JW

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Gjc1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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