Cyp4f6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp4f6
Entrez Gene ID	266689
Full Name	cytochrome P450, family 4, subfamily f, polypeptide 6
General protein information	Preferred Names cytochrome P450, family 4, subfamily f, polypeptide 6 Names cytochrome P450 4F6 CYPIVF6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	catalyzes the conversion of leukotriene B(4) into 19-hydoxy- and 18-hydroxy-leukotriene B(4); plays a role in inflammation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006241049.1	XP_006241111.1	cytochrome P450 4F6 isoform X2
NM_153318.1	NP_695230.1	cytochrome P450 4F6
XM_008765156.2	XP_008763378.1	cytochrome P450 4F6 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC748117	XP_001159781.2
Canis lupus familiaris (dog)	LOC484867	XP_541983.3
Mus musculus (house mouse)	Cyp4f13	NP_570952.1
Bos taurus (cattle)	LOC534967	NP_001039856.1
Rattus norvegicus (Norway rat)	Cyp4f6	NP_695230.1
	LOC718349	XP_001112302.1
Homo sapiens (human)	CYP4F3	NP_001186137.1

Gene Ontology
Bibliography

Related articles in PubMed

CYP4Fs expression in rat brain correlates with changes in LTB4 levels after traumatic brain injury.
Wang Y, Zhao J, Kalsotra A, Turman CM, Grill RJ, Dash PK, Strobel HW
Journal of neurotrauma25(10)1187-94(2008 Oct)

Renal localization, expression, and developmental regulation of P450 4F cytochromes in three substrains of spontaneously hypertensive rats.
Kalsotra A, Cui X, Anakk S, Hinojos CA, Doris PA, Strobel HW
Biochemical and biophysical research communications338(1)423-31(2005 Dec)

Leukotriene B4 omega-side chain hydroxylation by CYP4F5 and CYP4F6.
Bylund J, Harder AG, Maier KG, Roman RJ, Harder DR
Archives of biochemistry and biophysics412(1)34-41(2003 Apr)

cDNA cloning of three new forms of rat brain cytochrome P450 belonging to the CYP4F subfamily.
Kawashima H, Strobel HW
Biochemical and biophysical research communications217(3)1137-44(1995 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study shows that traumatic brain injury (TBI) triggers inflammation and elicits changes in mRNA expression of CYP4Fs in the frontal and occipital lobes and the hippocampus.
Title: CYP4Fs expression in rat brain correlates with changes in LTB4 levels after traumatic brain injury.

CYP4Fs are regionally distributed in the spontaneously hypertensive rat (SHR) kidney with CYP4F1, 4F4, and 4F5 being more highly expressed in the renal cortex than medulla while CYP4F6 shows higher medullary expression.
Title: Renal localization, expression, and developmental regulation of P450 4F cytochromes in three substrains of spontaneously hypertensive rats.

CYP4F5 and CYP4F6, due to their capacities to metabolize LTB(4), may play important roles in modulating inflammatory response in the lung and to some extent in the brain, kidney, and testis
Title: Leukotriene B4 omega-side chain hydroxylation by CYP4F5 and CYP4F6.

The following Cyp4f6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp4f6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03039	NM_153318.1 Latest version!	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa29946	XM_006241049.1 Latest version!	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa44507	XM_008765156.2 Latest version!	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp4f6 ( NM_153318.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_695230.1 Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), mRNA.

Price & Availability↑

CloneID	ORa03039
Clone ID Related Accession (Same CDS sequence)	NM_153318.1
Accession Version	NM_153318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 4F6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U39208.1. ##Evidence-Data-START## Transcript exon combination :: U39208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906353, SAMN01906357 [ECO:0000348] ##Evidence-Data-END##

ATGCTACAAC TAAGCCTGTC CCGGCTGGGA ATGGGGTCCC TGACAGCCTC TCCATGGCAT 
CTACTGCTGC TGGGAGGAGC CTCTTGGATA CTAGCCCGAA TTCTGGCCTG GATCTATACC 
TTCTATGACA ACTGCTGCCG CCTTCGTTGC TTCCCTCAGC CCCCTAAACC AAGTTGGTTT 
TGGGGTCACT TGACCTTGAT GAAGAACAAC GAGGAAGGCA TGCAGTTCAT AGCACATCTG 
GGCCGCAACT TCCGTGATAT CCACCTCTCT TGGGTGGGAC CCGTGTACCC GATCCTGCGA 
CTCGTCCACC CTAACGTCAT TGCTCCCCTG CTCCAAGCCT CAGCTGCTGT TGCACCCAAG 
GAAATGACCC TCTATGGCTT CCTGAAGCCC TGGCTGGGGG ATGGGCTCCT GATGAGCGCT 
GGTGAGAAGT GGAACCACCA CCGACGCCTG CTGACACCCG CCTTCCACTT TGACATCCTG 
AAGTCCTACG TGAAGATTTT TAACAAGAGC GTGAACACCA TGCATGCCAA GTGGCAGCGT 
CTGACTGCCA AGGGCAGTGC CCGTCTGGAC ATGTTCGAGC ACATCAGCCT GATGACCTTG 
GACAGCCTGC AAAAATGCAT CTTCAGCTTC GACAGCAACT GTCAGGAGTC TAACAGTGAA 
TACATAGCTG CGATCCTGGA ACTCAGCTCC CTCATAGTGA AACGGCAACG CCAGCCCTTC 
CTGTACCTGG ACTTCCTGTA TTACCTCACT GCTGATGGGC GGCGCTTCCG CAAGGCCTGC 
GACGTGGTGC ACAACTTCAC AGATGCTGTC ATCAGGGAGA GACGCAGCAC CCTCAATACC 
CAGGGCGTTG ATGAATTCCT AAAGGCCAGG GCTAAGACTA AAACTTTAGA CTTTATTGAT 
GTTCTCTTGC TGGCCAAGGA TGAGCATGGG AAGGGGCTGT CGGATGTGGA CATCCGAGCA 
GAGGCTGACA CCTTCATGTT CGGAGGTCAT GACACCACGG CCAGCGCACT CTCCTGGATC 
CTGTACAACC TGGCAAGGCA CCCGGAATAC CAGGAGCGCT GCCGGCAGGA GGTGCGGGAG 
CTGCTGAGGG ACCGAGAGCC TGAGGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTAACCATGT GCATCAAGGA GAGTCTGCGG CTGCACCCTC CAGTCTTATT AATCTCCCGC 
TGCTGTTCCC AGGACATTGT GCTGCCAGAT GGCCGGGTCA TCCCCAAAGG GAACATCTGT 
GTCATCAGCA TCTTTGGGGT TCACCACAAT CCTTCAGTGT GGCCAGACCC TGAGGTCTAC 
AACCCCTTCC GCTTTGACCC AGAAAACCCA CAGAAGAGGT CACCTCTGGC TTTTATTCCC 
TTCTCAGCGG GACCCAGGAA CTGCATAGGA CAGACTTTCG CCATGAGCGA GATAAAGGTG 
GCGCTGGCGC TGACGCTGCT GCGCTTCTGC GTCCTGCCAG ATGACAAGGA GCCGCGCCGG 
AAGCCGGAGC TGATCCTGCG TGCGGAGGGC GGACTGTGGC TGCGGGTGGA ACCGCTGAGC 
ACAGTGACCT CACAGCTGCC TTGGGACCTC CTCGCCCACC CTCCTACCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_695230.1
CDS	123..1736
Translation	MLQLSLSRLGMGSLTASPWHLLLLGGASWILARILAWIYTFYDNCCRLRCFPQPPKPSWFWGHLTLMKNNEEGMQFIAHLGRNFRDIHLSWVGPVYPILRLVHPNVIAPLLQASAAVAPKEMTLYGFLKPWLGDGLLMSAGEKWNHHRRLLTPAFHFDILKSYVKIFNKSVNTMHAKWQRLTAKGSARLDMFEHISLMTLDSLQKCIFSFDSNCQESNSEYIAAILELSSLIVKRQRQPFLYLDFLYYLTADGRRFRKACDVVHNFTDAVIRERRSTLNTQGVDEFLKARAKTKTLDFIDVLLLAKDEHGKGLSDVDIRAEADTFMFGGHDTTASALSWILYNLARHPEYQERCRQEVRELLRDREPEEIEWDDLAQLPFLTMCIKESLRLHPPVLLISRCCSQDIVLPDGRVIPKGNICVISIFGVHHNPSVWPDPEVYNPFRFDPENPQKRSPLAFIPFSAGPRNCIGQTFAMSEIKVALALTLLRFCVLPDDKEPRRKPELILRAEGGLWLRVEPLSTVTSQLPWDLLAHPPTS

Target ORF information:

RefSeq Version	NM_153318.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153318.1

ORF Insert Sequence:

ATGCTACAAC TAAGCCTGTC CCGGCTGGGA ATGGGGTCCC TGACAGCCTC TCCATGGCAT 
CTACTGCTGC TGGGAGGAGC CTCTTGGATA CTAGCCCGAA TTCTGGCCTG GATCTATACC 
TTCTATGACA ACTGCTGCCG CCTTCGTTGC TTCCCTCAGC CCCCTAAACC AAGTTGGTTT 
TGGGGTCACT TGACCTTGAT GAAGAACAAC GAGGAAGGCA TGCAGTTCAT AGCACATCTG 
GGCCGCAACT TCCGTGATAT CCACCTCTCT TGGGTGGGAC CCGTGTACCC GATCCTGCGA 
CTCGTCCACC CTAACGTCAT TGCTCCCCTG CTCCAAGCCT CAGCTGCTGT TGCACCCAAG 
GAAATGACCC TCTATGGCTT CCTGAAGCCC TGGCTGGGGG ATGGGCTCCT GATGAGCGCT 
GGTGAGAAGT GGAACCACCA CCGACGCCTG CTGACACCCG CCTTCCACTT TGACATCCTG 
AAGTCCTACG TGAAGATTTT TAACAAGAGC GTGAACACCA TGCATGCCAA GTGGCAGCGT 
CTGACTGCCA AGGGCAGTGC CCGTCTGGAC ATGTTCGAGC ACATCAGCCT GATGACCTTG 
GACAGCCTGC AAAAATGCAT CTTCAGCTTC GACAGCAACT GTCAGGAGTC TAACAGTGAA 
TACATAGCTG CGATCCTGGA ACTCAGCTCC CTCATAGTGA AACGGCAACG CCAGCCCTTC 
CTGTACCTGG ACTTCCTGTA TTACCTCACT GCTGATGGGC GGCGCTTCCG CAAGGCCTGC 
GACGTGGTGC ACAACTTCAC AGATGCTGTC ATCAGGGAGA GACGCAGCAC CCTCAATACC 
CAGGGCGTTG ATGAATTCCT AAAGGCCAGG GCTAAGACTA AAACTTTAGA CTTTATTGAT 
GTTCTCTTGC TGGCCAAGGA TGAGCATGGG AAGGGGCTGT CGGATGTGGA CATCCGAGCA 
GAGGCTGACA CCTTCATGTT CGGAGGTCAT GACACCACGG CCAGCGCACT CTCCTGGATC 
CTGTACAACC TGGCAAGGCA CCCGGAATAC CAGGAGCGCT GCCGGCAGGA GGTGCGGGAG 
CTGCTGAGGG ACCGAGAGCC TGAGGAGATT GAATGGGACG ACCTGGCCCA GCTGCCCTTC 
CTAACCATGT GCATCAAGGA GAGTCTGCGG CTGCACCCTC CAGTCTTATT AATCTCCCGC 
TGCTGTTCCC AGGACATTGT GCTGCCAGAT GGCCGGGTCA TCCCCAAAGG GAACATCTGT 
GTCATCAGCA TCTTTGGGGT TCACCACAAT CCTTCAGTGT GGCCAGACCC TGAGGTCTAC 
AACCCCTTCC GCTTTGACCC AGAAAACCCA CAGAAGAGGT CACCTCTGGC TTTTATTCCC 
TTCTCAGCGG GACCCAGGAA CTGCATAGGA CAGACTTTCG CCATGAGCGA GATAAAGGTG 
GCGCTGGCGC TGACGCTGCT GCGCTTCTGC GTCCTGCCAG ATGACAAGGA GCCGCGCCGG 
AAGCCGGAGC TGATCCTGCG TGCGGAGGGC GGACTGTGGC TGCGGGTGGA ACCGCTGAGC 
ACAGTGACCT CACAGCTGCC TTGGGACCTC CTCGCCCACC CTCCTACCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp4f6 ( XM_006241049.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241111.1 Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa29946
Clone ID Related Accession (Same CDS sequence)	XM_006241049.1
Accession Version	XM_006241049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 4F6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTAC AACTAAGCCT GTCCCGGCTG GGAATGGGGT CCCTGACAGC CTCTCCATGG 
CATCTACTGC TGCTGGGAGG AGCCTCTTGG ATACTAGCCC GAATTCTGGC CTGGATCTAT 
ACCTTCTATG ACAACTGCTG CCGCCTTCGT TGCTTCCCTC AGCCCCCTAA ACCAAGTTGG 
TTTTGGGGTC ACTTGACCTT GATGAAGAAC AACGAGGAAG GCATGCAGTT CATAGCACAT 
CTGGGCCGCA ACTTCCGTGA TATCCACCTC TCTTGGGTGG GACCCGTGTA CCCGATCCTG 
CGACTCGTCC ACCCTAACGT CATTGCTCCC CTGCTCCAAG CCTCAGCTGC TGTTGCACCC 
AAGGAAATGA CCCTCTATGG CTTCCTGAAG CCCTGGCTGG GGGATGGGCT CCTGATGAGC 
GCTGGTGAGA AGTGGAACCA CCACCGACGC CTGCTGACAC CCGCCTTCCA CTTTGACATC 
CTGAAGTCCT ACGTGAAGAT TTTTAACAAG AGCGTGAACA CCATGCATGC CAAGTGGCAG 
CGTCTGACTG CCAAGGGCAG TGCCCGTCTG GACATGTTCG AGCACATCAG CCTGATGACC 
TTGGACAGCC TGCAAAAATG CATCTTCAGC TTCGACAGCA ACTGTCAGGA GTCTAACAGT 
GAATACATAG CTGCGATCCT GGAACTCAGC TCCCTCATAG TGAAACGGCA ACGCCAGCCC 
TTCCTGTACC TGGACTTCCT GTATTACCTC ACTGCTGATG GGCGGCGCTT CCGCAAGGCC 
TGCGACGTGG TGCACAACTT CACAGATGCT GTCATCAGGG AGAGACGCAG CACCCTCAAT 
ACCCAGGGCG TTGATGAATT CCTAAAGGCC AGGGCTAAGA CTAAAACTTT AGACTTTATT 
GATGTTCTCT TGCTGGCCAA GGATGAGCAT GGGAAGGGGC TGTCGGATGT GGACATCCGA 
GCAGAGGCTG ACACCTTCAT GTTCGGAGGT CATGACACCA CGGCCAGCGC ACTCTCCTGG 
ATCCTGTACA ACCTGGCAAG GCACCCGGAA TACCAGGAGC GCTGCCGGCA GGAGGTGCGG 
GAGCTGCTGA GGGACCGAGA GCCTGAGGAG ATTGAATGGG ACGACCTGGC CCAGCTGCCC 
TTCCTAACCA TGTGCATCAA GGAGAGTCTG CGGCTGCACC CTCCAGTCTT ATTAATCTCC 
CGCTGCTGTT CCCAGGACAT TGTGCTGCCA GATGGCCGGG TCATCCCCAA AGGGAACATC 
TGTGTCATCA GCATCTTTGG GGTTCACCAC AATCCTTCAG TGTGGCCAGA CCCTGAGGTC 
TACAACCCCT TCCGCTTTGA CCCAGAAAAC CCACAGAAGA GGTCACCTCT GGCTTTTATT 
CCCTTCTCAG CGGGACCCAG GAACTGCATA GGACAGACTT TCGCCATGAG CGAGATAAAG 
GTGGCGCTGG CGCTGACGCT GCTGCGCTTC TGCGTCCTGC CAGATGACAA GGAGCCGCGC 
CGGAAGCCGG AGCTGATCCT GCGTGCGGAG GGCGGACTGT GGCTGCGGGT GGAACCGCTG 
AGCACAGTGA CCTCACAGCT GCCTTGGGAC CTCCTCGCCC ACCCTCCTAC CTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241111.1
CDS	194..1810
Translation	MMLQLSLSRLGMGSLTASPWHLLLLGGASWILARILAWIYTFYDNCCRLRCFPQPPKPSWFWGHLTLMKNNEEGMQFIAHLGRNFRDIHLSWVGPVYPILRLVHPNVIAPLLQASAAVAPKEMTLYGFLKPWLGDGLLMSAGEKWNHHRRLLTPAFHFDILKSYVKIFNKSVNTMHAKWQRLTAKGSARLDMFEHISLMTLDSLQKCIFSFDSNCQESNSEYIAAILELSSLIVKRQRQPFLYLDFLYYLTADGRRFRKACDVVHNFTDAVIRERRSTLNTQGVDEFLKARAKTKTLDFIDVLLLAKDEHGKGLSDVDIRAEADTFMFGGHDTTASALSWILYNLARHPEYQERCRQEVRELLRDREPEEIEWDDLAQLPFLTMCIKESLRLHPPVLLISRCCSQDIVLPDGRVIPKGNICVISIFGVHHNPSVWPDPEVYNPFRFDPENPQKRSPLAFIPFSAGPRNCIGQTFAMSEIKVALALTLLRFCVLPDDKEPRRKPELILRAEGGLWLRVEPLSTVTSQLPWDLLAHPPTS

Target ORF information:

RefSeq Version	XM_006241049.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241049.1

ORF Insert Sequence:

ATGATGCTAC AACTAAGCCT GTCCCGGCTG GGAATGGGGT CCCTGACAGC CTCTCCATGG 
CATCTACTGC TGCTGGGAGG AGCCTCTTGG ATACTAGCCC GAATTCTGGC CTGGATCTAT 
ACCTTCTATG ACAACTGCTG CCGCCTTCGT TGCTTCCCTC AGCCCCCTAA ACCAAGTTGG 
TTTTGGGGTC ACTTGACCTT GATGAAGAAC AACGAGGAAG GCATGCAGTT CATAGCACAT 
CTGGGCCGCA ACTTCCGTGA TATCCACCTC TCTTGGGTGG GACCCGTGTA CCCGATCCTG 
CGACTCGTCC ACCCTAACGT CATTGCTCCC CTGCTCCAAG CCTCAGCTGC TGTTGCACCC 
AAGGAAATGA CCCTCTATGG CTTCCTGAAG CCCTGGCTGG GGGATGGGCT CCTGATGAGC 
GCTGGTGAGA AGTGGAACCA CCACCGACGC CTGCTGACAC CCGCCTTCCA CTTTGACATC 
CTGAAGTCCT ACGTGAAGAT TTTTAACAAG AGCGTGAACA CCATGCATGC CAAGTGGCAG 
CGTCTGACTG CCAAGGGCAG TGCCCGTCTG GACATGTTCG AGCACATCAG CCTGATGACC 
TTGGACAGCC TGCAAAAATG CATCTTCAGC TTCGACAGCA ACTGTCAGGA GTCTAACAGT 
GAATACATAG CTGCGATCCT GGAACTCAGC TCCCTCATAG TGAAACGGCA ACGCCAGCCC 
TTCCTGTACC TGGACTTCCT GTATTACCTC ACTGCTGATG GGCGGCGCTT CCGCAAGGCC 
TGCGACGTGG TGCACAACTT CACAGATGCT GTCATCAGGG AGAGACGCAG CACCCTCAAT 
ACCCAGGGCG TTGATGAATT CCTAAAGGCC AGGGCTAAGA CTAAAACTTT AGACTTTATT 
GATGTTCTCT TGCTGGCCAA GGATGAGCAT GGGAAGGGGC TGTCGGATGT GGACATCCGA 
GCAGAGGCTG ACACCTTCAT GTTCGGAGGT CATGACACCA CGGCCAGCGC ACTCTCCTGG 
ATCCTGTACA ACCTGGCAAG GCACCCGGAA TACCAGGAGC GCTGCCGGCA GGAGGTGCGG 
GAGCTGCTGA GGGACCGAGA GCCTGAGGAG ATTGAATGGG ACGACCTGGC CCAGCTGCCC 
TTCCTAACCA TGTGCATCAA GGAGAGTCTG CGGCTGCACC CTCCAGTCTT ATTAATCTCC 
CGCTGCTGTT CCCAGGACAT TGTGCTGCCA GATGGCCGGG TCATCCCCAA AGGGAACATC 
TGTGTCATCA GCATCTTTGG GGTTCACCAC AATCCTTCAG TGTGGCCAGA CCCTGAGGTC 
TACAACCCCT TCCGCTTTGA CCCAGAAAAC CCACAGAAGA GGTCACCTCT GGCTTTTATT 
CCCTTCTCAG CGGGACCCAG GAACTGCATA GGACAGACTT TCGCCATGAG CGAGATAAAG 
GTGGCGCTGG CGCTGACGCT GCTGCGCTTC TGCGTCCTGC CAGATGACAA GGAGCCGCGC 
CGGAAGCCGG AGCTGATCCT GCGTGCGGAG GGCGGACTGT GGCTGCGGGT GGAACCGCTG 
AGCACAGTGA CCTCACAGCT GCCTTGGGAC CTCCTCGCCC ACCCTCCTAC CTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp4f6 ( XM_008765156.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763378.1 Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa44507
Clone ID Related Accession (Same CDS sequence)	XM_008765156.2
Accession Version	XM_008765156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 4F6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAATC CTGAGGATGC CCTACCTGAG TCTCTGACTC TCCCTCTATC TAATTCCTTC 
CCTTTAAGGT TGGACCCGCC CCCGCTCCCC TTGGCTGCCA GCGCAGGAGC AGAGGTTGAC 
GCTGAGGCTG GGAGCGATTG GCTGGTTCAG CCCAGCTCAA CTTCCCGCAC AGCTTCCGGC 
AAGTCGGAAG CCAGGGACAA AAAGTTTTCA AAGAAGATAG GAGGTTGTGG AGGACTCGCT 
GCTCATGAGA GAAGGATGCT ACAACTAAGC CTGTCCCGGC TGGGAATGGG GTCCCTGACA 
GCCTCTCCAT GGCATCTACT GCTGCTGGGA GGAGCCTCTT GGATACTAGC CCGAATTCTG 
GCCTGGATCT ATACCTTCTA TGACAACTGC TGCCGCCTTC GTTGCTTCCC TCAGCCCCCT 
AAACCAAGTT GGTTTTGGGG TCACTTGACC TTGATGAAGA ACAACGAGGA AGGCATGCAG 
TTCATAGCAC ATCTGGGCCG CAACTTCCGT GATATCCACC TCTCTTGGGT GGGACCCGTG 
TACCCGATCC TGCGACTCGT CCACCCTAAC GTCATTGCTC CCCTGCTCCA AGCCTCAGCT 
GCTGTTGCAC CCAAGGAAAT GACCCTCTAT GGCTTCCTGA AGCCCTGGCT GGGGGATGGG 
CTCCTGATGA GCGCTGGTGA GAAGTGGAAC CACCACCGAC GCCTGCTGAC ACCCGCCTTC 
CACTTTGACA TCCTGAAGTC CTACGTGAAG ATTTTTAACA AGAGCGTGAA CACCATGCAT 
GCCAAGTGGC AGCGTCTGAC TGCCAAGGGC AGTGCCCGTC TGGACATGTT CGAGCACATC 
AGCCTGATGA CCTTGGACAG CCTGCAAAAA TGCATCTTCA GCTTCGACAG CAACTGTCAG 
GAGTCTAACA GTGAATACAT AGCTGCGATC CTGGAACTCA GCTCCCTCAT AGTGAAACGG 
CAACGCCAGC CCTTCCTGTA CCTGGACTTC CTGTATTACC TCACTGCTGA TGGGCGGCGC 
TTCCGCAAGG CCTGCGACGT GGTGCACAAC TTCACAGATG CTGTCATCAG GGAGAGACGC 
AGCACCCTCA ATACCCAGGG CGTTGATGAA TTCCTAAAGG CCAGGGCTAA GACTAAAACT 
TTAGACTTTA TTGATGTTCT CTTGCTGGCC AAGGATGAGC ATGGGAAGGG GCTGTCGGAT 
GTGGACATCC GAGCAGAGGC TGACACCTTC ATGTTCGGAG GTCATGACAC CACGGCCAGC 
GCACTCTCCT GGATCCTGTA CAACCTGGCA AGGCACCCGG AATACCAGGA GCGCTGCCGG 
CAGGAGGTGC GGGAGCTGCT GAGGGACCGA GAGCCTGAGG AGATTGAATG GGACGACCTG 
GCCCAGCTGC CCTTCCTAAC CATGTGCATC AAGGAGAGTC TGCGGCTGCA CCCTCCAGTC 
TTATTAATCT CCCGCTGCTG TTCCCAGGAC ATTGTGCTGC CAGATGGCCG GGTCATCCCC 
AAAGGGAACA TCTGTGTCAT CAGCATCTTT GGGGTTCACC ACAATCCTTC AGTGTGGCCA 
GACCCTGAGG TCTACAACCC CTTCCGCTTT GACCCAGAAA ACCCACAGAA GAGGTCACCT 
CTGGCTTTTA TTCCCTTCTC AGCGGGACCC AGGAACTGCA TAGGACAGAC TTTCGCCATG 
AGCGAGATAA AGGTGGCGCT GGCGCTGACG CTGCTGCGCT TCTGCGTCCT GCCAGATGAC 
AAGGAGCCGC GCCGGAAGCC GGAGCTGATC CTGCGTGCGG AGGGCGGACT GTGGCTGCGG 
GTGGAACCGC TGAGCACAGT GACCTCACAG CTGCCTTGGG ACCTCCTCGC CCACCCTCCT 
ACCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763378.1
CDS	16..1884
Translation	MLNPEDALPESLTLPLSNSFPLRLDPPPLPLAASAGAEVDAEAGSDWLVQPSSTSRTASGKSEARDKKFSKKIGGCGGLAAHERRMLQLSLSRLGMGSLTASPWHLLLLGGASWILARILAWIYTFYDNCCRLRCFPQPPKPSWFWGHLTLMKNNEEGMQFIAHLGRNFRDIHLSWVGPVYPILRLVHPNVIAPLLQASAAVAPKEMTLYGFLKPWLGDGLLMSAGEKWNHHRRLLTPAFHFDILKSYVKIFNKSVNTMHAKWQRLTAKGSARLDMFEHISLMTLDSLQKCIFSFDSNCQESNSEYIAAILELSSLIVKRQRQPFLYLDFLYYLTADGRRFRKACDVVHNFTDAVIRERRSTLNTQGVDEFLKARAKTKTLDFIDVLLLAKDEHGKGLSDVDIRAEADTFMFGGHDTTASALSWILYNLARHPEYQERCRQEVRELLRDREPEEIEWDDLAQLPFLTMCIKESLRLHPPVLLISRCCSQDIVLPDGRVIPKGNICVISIFGVHHNPSVWPDPEVYNPFRFDPENPQKRSPLAFIPFSAGPRNCIGQTFAMSEIKVALALTLLRFCVLPDDKEPRRKPELILRAEGGLWLRVEPLSTVTSQLPWDLLAHPPTS

Target ORF information:

RefSeq Version	XM_008765156.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 4, subfamily f, polypeptide 6 (Cyp4f6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765156.2

ORF Insert Sequence:

ATGTTAAATC CTGAGGATGC CCTACCTGAG TCTCTGACTC TCCCTCTATC TAATTCCTTC 
CCTTTAAGGT TGGACCCGCC CCCGCTCCCC TTGGCTGCCA GCGCAGGAGC AGAGGTTGAC 
GCTGAGGCTG GGAGCGATTG GCTGGTTCAG CCCAGCTCAA CTTCCCGCAC AGCTTCCGGC 
AAGTCGGAAG CCAGGGACAA AAAGTTTTCA AAGAAGATAG GAGGTTGTGG AGGACTCGCT 
GCTCATGAGA GAAGGATGCT ACAACTAAGC CTGTCCCGGC TGGGAATGGG GTCCCTGACA 
GCCTCTCCAT GGCATCTACT GCTGCTGGGA GGAGCCTCTT GGATACTAGC CCGAATTCTG 
GCCTGGATCT ATACCTTCTA TGACAACTGC TGCCGCCTTC GTTGCTTCCC TCAGCCCCCT 
AAACCAAGTT GGTTTTGGGG TCACTTGACC TTGATGAAGA ACAACGAGGA AGGCATGCAG 
TTCATAGCAC ATCTGGGCCG CAACTTCCGT GATATCCACC TCTCTTGGGT GGGACCCGTG 
TACCCGATCC TGCGACTCGT CCACCCTAAC GTCATTGCTC CCCTGCTCCA AGCCTCAGCT 
GCTGTTGCAC CCAAGGAAAT GACCCTCTAT GGCTTCCTGA AGCCCTGGCT GGGGGATGGG 
CTCCTGATGA GCGCTGGTGA GAAGTGGAAC CACCACCGAC GCCTGCTGAC ACCCGCCTTC 
CACTTTGACA TCCTGAAGTC CTACGTGAAG ATTTTTAACA AGAGCGTGAA CACCATGCAT 
GCCAAGTGGC AGCGTCTGAC TGCCAAGGGC AGTGCCCGTC TGGACATGTT CGAGCACATC 
AGCCTGATGA CCTTGGACAG CCTGCAAAAA TGCATCTTCA GCTTCGACAG CAACTGTCAG 
GAGTCTAACA GTGAATACAT AGCTGCGATC CTGGAACTCA GCTCCCTCAT AGTGAAACGG 
CAACGCCAGC CCTTCCTGTA CCTGGACTTC CTGTATTACC TCACTGCTGA TGGGCGGCGC 
TTCCGCAAGG CCTGCGACGT GGTGCACAAC TTCACAGATG CTGTCATCAG GGAGAGACGC 
AGCACCCTCA ATACCCAGGG CGTTGATGAA TTCCTAAAGG CCAGGGCTAA GACTAAAACT 
TTAGACTTTA TTGATGTTCT CTTGCTGGCC AAGGATGAGC ATGGGAAGGG GCTGTCGGAT 
GTGGACATCC GAGCAGAGGC TGACACCTTC ATGTTCGGAG GTCATGACAC CACGGCCAGC 
GCACTCTCCT GGATCCTGTA CAACCTGGCA AGGCACCCGG AATACCAGGA GCGCTGCCGG 
CAGGAGGTGC GGGAGCTGCT GAGGGACCGA GAGCCTGAGG AGATTGAATG GGACGACCTG 
GCCCAGCTGC CCTTCCTAAC CATGTGCATC AAGGAGAGTC TGCGGCTGCA CCCTCCAGTC 
TTATTAATCT CCCGCTGCTG TTCCCAGGAC ATTGTGCTGC CAGATGGCCG GGTCATCCCC 
AAAGGGAACA TCTGTGTCAT CAGCATCTTT GGGGTTCACC ACAATCCTTC AGTGTGGCCA 
GACCCTGAGG TCTACAACCC CTTCCGCTTT GACCCAGAAA ACCCACAGAA GAGGTCACCT 
CTGGCTTTTA TTCCCTTCTC AGCGGGACCC AGGAACTGCA TAGGACAGAC TTTCGCCATG 
AGCGAGATAA AGGTGGCGCT GGCGCTGACG CTGCTGCGCT TCTGCGTCCT GCCAGATGAC 
AAGGAGCCGC GCCGGAAGCC GGAGCTGATC CTGCGTGCGG AGGGCGGACT GTGGCTGCGG 
GTGGAACCGC TGAGCACAGT GACCTCACAG CTGCCTTGGG ACCTCCTCGC CCACCCTCCT 
ACCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CYP4Fs expression in rat brain correlates with changes in LTB4 levels after traumatic brain injury.
Journal of neurotrauma25(10)1187-94(2008 Oct)
Wang Y,Zhao J,Kalsotra A,Turman CM,Grill RJ,Dash PK,Strobel HW

Renal localization, expression, and developmental regulation of P450 4F cytochromes in three substrains of spontaneously hypertensive rats.
Biochemical and biophysical research communications338(1)423-31(2005 Dec)
Kalsotra A,Cui X,Anakk S,Hinojos CA,Doris PA,Strobel HW

Leukotriene B4 omega-side chain hydroxylation by CYP4F5 and CYP4F6.
Archives of biochemistry and biophysics412(1)34-41(2003 Apr)
Bylund J,Harder AG,Maier KG,Roman RJ,Harder DR

cDNA cloning of three new forms of rat brain cytochrome P450 belonging to the CYP4F subfamily.
Biochemical and biophysical research communications217(3)1137-44(1995 Dec)
Kawashima H,Strobel HW

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Cyp4f6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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