gRNAs
The following Cenpf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cenpf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa30197 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001100827.1 | |
| Accession Version | NM_001100827.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 8958bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-18 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | centromere protein F | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07022106.1, DY549432.1 and EV780064.1. On or before Dec 19, 2009 this sequence version replaced XM_001067266.1, XM_223060.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2689599, SAMN01906349 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAGCTGGG CGTTGGAAGA GTGGAAGGAA GGTCTCCCGA CCAGAGCTCT TCAGAAAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001094297.1 |
| CDS | 143..9100 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001100827.1 |
1 | ATGAGCTGGG CGTTGGAAGA GTGGAAGGAA GGTCTCCCGA CCAGAGCTCT TCAGAAAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The kinetochore protein, CENPF, is mutated in human ciliopathy and microcephaly phenotypes. |
Cenp-F links kinetochores to Ndel1/Nde1/Lis1/dynein microtubule motor complexes. |
The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions. |
Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F. |
Silencing mitosin induces misaligned chromosomes, premature chromosome decondensation before anaphase onset, and mitotic cell death. |
