Clcn5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clcn5
Entrez Gene ID	25749
Full Name	chloride voltage-gated channel 5
Synonyms	CLC5
General protein information	Preferred Names chloride voltage-gated channel 5 Names H(+)/Cl(-) exchange transporter 5 chloride channel 5 chloride channel protein 5 chloride channel, voltage-sensitive 5 chloride transporter ClC-5 clC-5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq12
Summary	acts as an outwardly rectifying chloride channel; plays a role in chloride transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017106.1	NP_058802.1	H(+)/Cl(-) exchange transporter 5

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Clcn5	NP_001230691.1
Rattus norvegicus (Norway rat)	Clcn5	NP_058802.1
	CLCN5	XP_001083186.2
Pan troglodytes (chimpanzee)	CLCN5	XP_003317509.2
Danio rerio (zebrafish)	si:dkey-22f5.8	XP_001920783.2
Homo sapiens (human)	CLCN5	NP_001121371.1
Canis lupus familiaris (dog)	CLCN5	XP_005641410.1
Bos taurus (cattle)	CLCN5	XP_005228237.1
Gallus gallus (chicken)	CLCN5	XP_004940778.1
Danio rerio (zebrafish)	LOC557584	XP_685762.2
Xenopus tropicalis (tropical clawed frog)	clcn5	NP_001039210.1

Gene Ontology
Bibliography

Related articles in PubMed

Diet induced obesity in rats reduces NHE3 and Na(+) /K(+) -ATPase expression in the kidney.
Briffa JF, Grinfeld E, Jenkin KA, Mathai ML, Poronnik P, McAinch AJ, Hryciw DH
Clinical and experimental pharmacology & physiology42(10)1118-26(2015 Oct)

Chloride channel ClC-5 binds to aspartyl aminopeptidase to regulate renal albumin endocytosis.
Lee A, Slattery C, Nikolic-Paterson DJ, Hryciw DH, Wilk S, Wilk E, Zhang Y, Valova VA, Robinson PJ, Kelly DJ, Poronnik P
American journal of physiology. Renal physiology308(7)F784-92(2015 Apr)

WDR72 models of structure and function: a stage-specific regulator of enamel mineralization.
Katsura KA, Horst JA, Chandra D, Le TQ, Nakano Y, Zhang Y, Horst OV, Zhu L, Le MH, DenBesten PK
Matrix biology : journal of the International Society for Matrix Biology3848-58(2014 Sep)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Role of CFTR and ClC-5 in modulating vacuolar H+-ATPase activity in kidney proximal tubule.
Carraro-Lacroix LR, Lessa LM, Bezerra CN, Pessoa TD, Souza-Menezes J, Morales MM, Girardi AC, Malnic G
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology26(4-5)563-76(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified another key molecular component of the albumin endocytic machinery in the renal proximal tubule and describe a new role for aspartyl aminopeptidase in stabilizing the actin cytoskeleton
Title: Chloride channel ClC-5 binds to aspartyl aminopeptidase to regulate renal albumin endocytosis.

ClC-5 chloride-proton exchangers by siRNA-mediated silencing are important modulators of H+-ATPase function in immortalized rat renal proximal tubule cells.
Title: Role of CFTR and ClC-5 in modulating vacuolar H+-ATPase activity in kidney proximal tubule.

ClC-5 is expressed exclusively in H(+)-ATPase-rich cells (narrow and clear cells). ClC-5 also partially colocalizes with the H(+)-ATPase in the subapical pole of clear cells in the epididymis.
Title: Detection of ClC-3 and ClC-5 in epididymal epithelium: immunofluorescence and RT-PCR after LCM.

The following Clcn5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcn5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02865	NM_017106.1 Latest version!	Rattus norvegicus chloride voltage-gated channel 5 (Clcn5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clcn5 ( NM_017106.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058802.1 Rattus norvegicus chloride voltage-gated channel 5 (Clcn5), mRNA.

Price & Availability↑

CloneID	ORa02865
Clone ID Related Accession (Same CDS sequence)	NM_017106.1
Accession Version	NM_017106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	H(+)/Cl(-) exchange transporter 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D50497.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D50497.1, Z56277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACTTCT TGGAGGAGCC AATCCCCGGT GTAGGGACCT ATGATGATTT CAACACAATT 
GATTGGGTGA GAGAGAAATC TCGAGACCGA GATAGGCACC GAGAGATTAC CAATAAAAGC 
AAAGAGTCAA CATGGGCCTT AATTCACAGT GTGAGCGATG CTTTTTCTGG TTGGTTGTTA 
ATGCTCCTTA TTGGCCTTTT ATCAGGTTCC TTAGCTGGCC TCATAGACAT CTCTGCTCAC 
TGGATGACAG ACTTAAAAGA AGGGATATGC ACAGGGGGCT TCTGGTTTAA CCATGAGCAC 
TGCTGCTGGA ATTCCGAGCA TGTCACCTTT GAAGACAGAG ACAAATGTCC GGAGTGGAAT 
AGCTGGTCCC AGCTTATCAT CAGCACAGAT CAGGGAGCCT TTGCCTACAT AGTCAATTAT 
TTCATGTATG TCCTCTGGGC TCTCCTGTTT GCTTTCCTCG CTGTCTCTCT TGTCAAGGCA 
TTTGCACCTT ACGCCTGTGG TTCTGGAATC CCTGAGATCA AAACTATCCT GAGTGGTTTC 
ATTATTAGGG GCTATTTGGG TAAGTGGACC CTTGTCATCA AAACTATTAC TTTGGTGCTG 
GCCGTGTCAT CTGGCTTGAG CCTGGGTAAA GAGGGCCCCC TAGTGCACGT GGCTTGCTGC 
TGTGGGAACA TCCTGTGCCA CTGCTTCAAC AAATACAGGA AGAATGAAGC CAAGCGCAGA 
GAGGTCTTAT CAGCTGCAGC AGCTGCTGGT GTATCTGTAG CCTTTGGGGC CCCTATTGGT 
GGAGTATTGT TCAGCCTGGA AGAGGTCAGC TACTACTTTC CCCTCAAAAC ACTGTGGCGT 
TCCTTCTTCG CTGCTCTCGT GGCAGCATTC ACGCTCCGCT CCATCAATCC ATTTGGAAAC 
AGCCGCTTAG TTCTATTTTA TGTGGAGTTC CACACCCCAT GGTATCTCTT TGAGCTCGTG 
CCATTCATTG TGCTAGGCAT ATTCGGTGGC CTTTGGGGCG CTCTGTTTAT CCGCACAAAC 
ATTGCCTGGT GTCGGAAGCG TAAAACCACA CAGTTGGGCA AGTATCCTGT TGTCGAGGTC 
CTCATTGTGA CAGCCATCAC TGCCATCTTG GCTTTCCCCA ATGAATATAC CCGGATGAGC 
ACAAGTGAGC TCATTTCTGA ACTGTTCAAT GACTGCGGCC TCCTGGACTC CTCCAAGCTC 
TGTGATTATG AGAACCACTT CAACACTAGC AAAGGCGGTG AGCTGCCTGA CAGACCTGCT 
GGCGTGGGAG TCTACAGTGC CATGTGGCAG CTGGCTTTGA CACTCATACT GAAAATAGTC 
ATTACTATAT TCACCTTTGG CATGAAGATT CCTTCTGGTC TCTTTATCCC CAGCATGGCT 
GTCGGTGCTA TAGCAGGCCG CCTTTTAGGA GTCGGAATGG AGCAACTGGC TTATTACCAC 
CACGACTGGG GCATCTTCAA TAGCTGGTGT AGCCAGGGAG CGGATTGCAT CACGCCTGGC 
CTTTATGCCA TGGTGGGGGC TGCCGCCTGC TTAGGCGGTG TGACTCGGAT GACTGTTTCT 
CTCGTTGTCA TAATGTTTGA GCTCACTGGT GGCCTGGAAT ATATTGTACC TCTGATGGCT 
GCAGCCATGA CGAGCAAGTG GGTGGCAGAC GCTCTTGGGC GAGAGGGCAT TTATGATGCC 
CACATCCGTC TCAATGGATA CCCCTTTCTC GAAGCCAAAG AAGAATTTGC TCATAAGACT 
CTGGCAATGG ATGTGATGAA GCCCCGGAGG AATGATCCCT TGCTGACTGT CCTTACTCAG 
GACAGTATGA CTGTGGAAGA TGTAGAGACC ATAATCAGTG AGACCACGTA CAGCGGCTTT 
CCGGTGGTGG TGTCCCGAGA GTCACAAAGA CTTGTAGGCT TTGTTCTTCG GAGAGATCTC 
ATTATTTCAA TTGAAAATGC TCGGAAGAAA CAGGACGGGG TTGTGAGCAC GTCCATCATT 
TATTTCACGG AGCACTCACC CCCAATGCCT CCATACACGC CCCCTACCCT GAAGCTTCGG 
AACATCCTGG ATCTCAGCCC TTTCACTGTG ACAGACCTTA CGCCAATGGA GATCGTAGTG 
GATATCTTCC GCAAGCTGGG ACTGCGCCAG TGCCTGGTTA CACACAATGG GCGCCTACTT 
GGAATCATTA CCAAAAAGGA TGTGTTAAAG CACATAGCGC AGATGGCAAA CCAAGACCCC 
GAGTCCATTC TCTTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058802.1
CDS	211..2451
Translation	MDFLEEPIPGVGTYDDFNTIDWVREKSRDRDRHREITNKSKESTWALIHSVSDAFSGWLLMLLIGLLSGSLAGLIDISAHWMTDLKEGICTGGFWFNHEHCCWNSEHVTFEDRDKCPEWNSWSQLIISTDQGAFAYIVNYFMYVLWALLFAFLAVSLVKAFAPYACGSGIPEIKTILSGFIIRGYLGKWTLVIKTITLVLAVSSGLSLGKEGPLVHVACCCGNILCHCFNKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWYLFELVPFIVLGIFGGLWGALFIRTNIAWCRKRKTTQLGKYPVVEVLIVTAITAILAFPNEYTRMSTSELISELFNDCGLLDSSKLCDYENHFNTSKGGELPDRPAGVGVYSAMWQLALTLILKIVITIFTFGMKIPSGLFIPSMAVGAIAGRLLGVGMEQLAYYHHDWGIFNSWCSQGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADALGREGIYDAHIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQRLVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPMPPYTPPTLKLRNILDLSPFTVTDLTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPESILFN

Target ORF information:

RefSeq Version	NM_017106.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chloride voltage-gated channel 5 (Clcn5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017106.1

ORF Insert Sequence:

ATGGACTTCT TGGAGGAGCC AATCCCCGGT GTAGGGACCT ATGATGATTT CAACACAATT 
GATTGGGTGA GAGAGAAATC TCGAGACCGA GATAGGCACC GAGAGATTAC CAATAAAAGC 
AAAGAGTCAA CATGGGCCTT AATTCACAGT GTGAGCGATG CTTTTTCTGG TTGGTTGTTA 
ATGCTCCTTA TTGGCCTTTT ATCAGGTTCC TTAGCTGGCC TCATAGACAT CTCTGCTCAC 
TGGATGACAG ACTTAAAAGA AGGGATATGC ACAGGGGGCT TCTGGTTTAA CCATGAGCAC 
TGCTGCTGGA ATTCCGAGCA TGTCACCTTT GAAGACAGAG ACAAATGTCC GGAGTGGAAT 
AGCTGGTCCC AGCTTATCAT CAGCACAGAT CAGGGAGCCT TTGCCTACAT AGTCAATTAT 
TTCATGTATG TCCTCTGGGC TCTCCTGTTT GCTTTCCTCG CTGTCTCTCT TGTCAAGGCA 
TTTGCACCTT ACGCCTGTGG TTCTGGAATC CCTGAGATCA AAACTATCCT GAGTGGTTTC 
ATTATTAGGG GCTATTTGGG TAAGTGGACC CTTGTCATCA AAACTATTAC TTTGGTGCTG 
GCCGTGTCAT CTGGCTTGAG CCTGGGTAAA GAGGGCCCCC TAGTGCACGT GGCTTGCTGC 
TGTGGGAACA TCCTGTGCCA CTGCTTCAAC AAATACAGGA AGAATGAAGC CAAGCGCAGA 
GAGGTCTTAT CAGCTGCAGC AGCTGCTGGT GTATCTGTAG CCTTTGGGGC CCCTATTGGT 
GGAGTATTGT TCAGCCTGGA AGAGGTCAGC TACTACTTTC CCCTCAAAAC ACTGTGGCGT 
TCCTTCTTCG CTGCTCTCGT GGCAGCATTC ACGCTCCGCT CCATCAATCC ATTTGGAAAC 
AGCCGCTTAG TTCTATTTTA TGTGGAGTTC CACACCCCAT GGTATCTCTT TGAGCTCGTG 
CCATTCATTG TGCTAGGCAT ATTCGGTGGC CTTTGGGGCG CTCTGTTTAT CCGCACAAAC 
ATTGCCTGGT GTCGGAAGCG TAAAACCACA CAGTTGGGCA AGTATCCTGT TGTCGAGGTC 
CTCATTGTGA CAGCCATCAC TGCCATCTTG GCTTTCCCCA ATGAATATAC CCGGATGAGC 
ACAAGTGAGC TCATTTCTGA ACTGTTCAAT GACTGCGGCC TCCTGGACTC CTCCAAGCTC 
TGTGATTATG AGAACCACTT CAACACTAGC AAAGGCGGTG AGCTGCCTGA CAGACCTGCT 
GGCGTGGGAG TCTACAGTGC CATGTGGCAG CTGGCTTTGA CACTCATACT GAAAATAGTC 
ATTACTATAT TCACCTTTGG CATGAAGATT CCTTCTGGTC TCTTTATCCC CAGCATGGCT 
GTCGGTGCTA TAGCAGGCCG CCTTTTAGGA GTCGGAATGG AGCAACTGGC TTATTACCAC 
CACGACTGGG GCATCTTCAA TAGCTGGTGT AGCCAGGGAG CGGATTGCAT CACGCCTGGC 
CTTTATGCCA TGGTGGGGGC TGCCGCCTGC TTAGGCGGTG TGACTCGGAT GACTGTTTCT 
CTCGTTGTCA TAATGTTTGA GCTCACTGGT GGCCTGGAAT ATATTGTACC TCTGATGGCT 
GCAGCCATGA CGAGCAAGTG GGTGGCAGAC GCTCTTGGGC GAGAGGGCAT TTATGATGCC 
CACATCCGTC TCAATGGATA CCCCTTTCTC GAAGCCAAAG AAGAATTTGC TCATAAGACT 
CTGGCAATGG ATGTGATGAA GCCCCGGAGG AATGATCCCT TGCTGACTGT CCTTACTCAG 
GACAGTATGA CTGTGGAAGA TGTAGAGACC ATAATCAGTG AGACCACGTA CAGCGGCTTT 
CCGGTGGTGG TGTCCCGAGA GTCACAAAGA CTTGTAGGCT TTGTTCTTCG GAGAGATCTC 
ATTATTTCAA TTGAAAATGC TCGGAAGAAA CAGGACGGGG TTGTGAGCAC GTCCATCATT 
TATTTCACGG AGCACTCACC CCCAATGCCT CCATACACGC CCCCTACCCT GAAGCTTCGG 
AACATCCTGG ATCTCAGCCC TTTCACTGTG ACAGACCTTA CGCCAATGGA GATCGTAGTG 
GATATCTTCC GCAAGCTGGG ACTGCGCCAG TGCCTGGTTA CACACAATGG GCGCCTACTT 
GGAATCATTA CCAAAAAGGA TGTGTTAAAG CACATAGCGC AGATGGCAAA CCAAGACCCC 
GAGTCCATTC TCTTCAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diet induced obesity in rats reduces NHE3 and Na(+) /K(+) -ATPase expression in the kidney.
Clinical and experimental pharmacology & physiology42(10)1118-26(2015 Oct)
Briffa JF,Grinfeld E,Jenkin KA,Mathai ML,Poronnik P,McAinch AJ,Hryciw DH

Chloride channel ClC-5 binds to aspartyl aminopeptidase to regulate renal albumin endocytosis.
American journal of physiology. Renal physiology308(7)F784-92(2015 Apr)
Lee A,Slattery C,Nikolic-Paterson DJ,Hryciw DH,Wilk S,Wilk E,Zhang Y,Valova VA,Robinson PJ,Kelly DJ,Poronnik P

WDR72 models of structure and function: a stage-specific regulator of enamel mineralization.
Matrix biology : journal of the International Society for Matrix Biology3848-58(2014 Sep)
Katsura KA,Horst JA,Chandra D,Le TQ,Nakano Y,Zhang Y,Horst OV,Zhu L,Le MH,DenBesten PK

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Role of CFTR and ClC-5 in modulating vacuolar H+-ATPase activity in kidney proximal tubule.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology26(4-5)563-76(2010)
Carraro-Lacroix LR,Lessa LM,Bezerra CN,Pessoa TD,Souza-Menezes J,Morales MM,Girardi AC,Malnic G

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Clcn5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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