Alas2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alas2
Entrez Gene ID	25748
Full Name	5'-aminolevulinate synthase 2
General protein information	Preferred Names 5'-aminolevulinate synthase 2 Names 5-aminolevulinate synthase, erythroid-specific, mitochondrial 5-aminolevulinic acid synthase 2 ALAS-E Aminolevulinate synthase 2, delta aminolevulinate, delta-, synthase 2 aminolevulinic acid synthase 2, erythroid aminolevulinic acid synthase 2, erythroid-like delta-ALA synthase 2 delta-ALA synthetase delta-aminolevulinate synthase 2 erythroid-specific delta-aminolevulinate synthase ALAS-E
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq13
Summary	erythroid-specific isozyme that catalyzes the conversion of glycine and succinyl-CoA to 5-aminolevulinate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013197.1	NP_037329.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Alas	NP_477281.1
Xenopus tropicalis (tropical clawed frog)	alas2	NP_001006926.1
Canis lupus familiaris (dog)	ALAS2	XP_548619.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003184	XP_312882.5
Saccharomyces cerevisiae (baker's yeast)	HEM1	NP_010518.1
Eremothecium gossypii	AGOS_ABL104C	NP_982843.1
Danio rerio (zebrafish)	alas2	NP_571757.1
Magnaporthe oryzae (rice blast fungus)	MGG_06446	XP_003717121.1
Bos taurus (cattle)	ALAS2	NP_001030275.1
Rattus norvegicus (Norway rat)	Alas2	NP_037329.1
Schizosaccharomyces pombe (fission yeast)	hem1	NP_594388.1
Neurospora crassa	NCU06189	XP_963001.2
Homo sapiens (human)	ALAS2	NP_000023.2
Pan troglodytes (chimpanzee)	ALAS2	XP_528998.2
	ALAS2	XP_002806298.1
Mus musculus (house mouse)	Alas2	NP_033783.1
Kluyveromyces lactis	KLLA0C15059g	XP_452875.1

Gene Ontology
Bibliography

Related articles in PubMed

Synthesis, delivery and regulation of eukaryotic heme and Fe-S cluster cofactors.
Barupala DP, Dzul SP, Riggs-Gelasco PJ, Stemmler TL
Archives of biochemistry and biophysics59260-75(2016 Feb)

ALAS2 acts as a modifier gene in patients with congenital erythropoietic porphyria.
To-Figueras J, Ducamp S, Clayton J, Badenas C, Delaby C, Ged C, Lyoumi S, Gouya L, de Verneuil H, Beaumont C, Ferreira GC, Deybach JC, Herrero C, Puy H
Blood118(6)1443-51(2011 Aug)

A toxicogenomic approach for identifying biomarkers for myelosuppressive anemia in rats.
Uehara T, Kondo C, Yamate J, Torii M, Maruyama T
Toxicology282(3)139-45(2011 Apr)

New mutation in erythroid-specific delta-aminolevulinate synthase as the cause of X-linked sideroblastic anemia responsive to pyridoxine.
Kucerova J, Horvathova M, Mojzikova R, Belohlavkova P, Cermak J, Divoky V
Acta haematologica125(4)193-7(2011)

Fish oil feeding up-regulates the expression of 5-aminolevulinate synthase 2 mRNA in rat brain.
Haraguchi T, Yanaka N, Eguchi Y, Kudo T, Hirata A, Kato N
Bioscience, biotechnology, and biochemistry75(7)1383-5(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Alas2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alas2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12927	NM_013197.1 Latest version!	Rattus norvegicus 5'-aminolevulinate synthase 2 (Alas2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alas2 ( NM_013197.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037329.1 Rattus norvegicus 5'-aminolevulinate synthase 2 (Alas2), mRNA.

Price & Availability↑

CloneID	ORa12927
Clone ID Related Accession (Same CDS sequence)	NM_013197.1
Accession Version	NM_013197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D86297.1. On Jun 25, 2006 this sequence version replaced XM_001062452.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86297.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349, SAMN01906350 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGTGGCAG CAGCTATGTT GCTACGGTCC TGTCCAGTGC TCTCTAAGGG CCCCACAGGC 
CTCCTAGGCA AAGTGGCTAA AACTTACCAG TTCCTATTTG GTATTGGACG CTGCCCCATC 
CTGGCCACTC AAGGACCAAC CTGTTCTCAA ATCCATCTTA AGGCAACCAA GGCTGGAGCA 
GACTCTCCGT CTTGGACTAA GAGCCATTGT CCTTTCATGC TGTCAGAACT CCAAGACAGG 
AAGAGCAAGA TTGTGCAGAG GGCAGCTCCA GAAGTCCAAG AGGATGTCAA GACTTTCAAG 
ACAGACCTGC TGAGCTTCAT GGAGTCAACT ACCCGAAGCC AATCAGTGCC TCGTTTCCAG 
GATCCAGAGC AGACTGGAGG GGCACCTCCC CTGCTGATTC AGAATAATAT GACTGGAAGC 
CAGGCTTTTG GTTATGACCA ATTTTTTAGA GACAAGATCA TGGAAAAGAA GCAGGACCAC 
ACATACCGTG TGTTCAAGAC TGTGAATCGC TGGGCTAATG CCTACCCCTT TGCCCAACAC 
TTCTCCGAGG CATCTATGGA CTCAAAGGAT GTTTCTGTCT GGTGCAGTAA TGACTATTTG 
GGCATAAGCA GGCACCCTCG TGTCTTGCAG GCCATAGAGG AGACCCTGAA GAATCATGGA 
GCTGGAGCTG GGGGCACTCG CAATATCTCA GGTACAAGCA AGTTTCATGT GGAGCTTGAA 
CAGGAGCTGG CTGAACTACA TCATAAGGAC TCGGCTCTGC TCTTCTCCTC CTGTTTTGTG 
GCCAATGATT CTACTCTCTT TACACTGGCC AAGCTTCTGC CAGGGTGTGA GATCTACTCA 
GACGCAGGCA ATCATGCCTC CATGATCCAA GGCATTCGTA ACAGTGGTGC AGTCAAGTTT 
GTCTTCAGAC ACAATGACCC AGGCCACCTG AAGAAACTTC TTGAGAAGTC CGATCCCAAG 
ACACCAAAAA TTGTGGCTTT TGAGACTGTT CACTCCATGG ATGGTGCCAT CTGTCCTCTG 
GAGGAATTGT GTGATGTGGC CCACCAGTAC GGGGCCCTGA CCTTCGTGGA TGAAGTCCAT 
GCCGTAGGAC TATATGGAAC CCGGGGTGCA GGGATCGGGG AGCGTGATGG AATTATGCAC 
AAGCTTGACA TCATCTCTGG AACTCTTGGC AAGGCCTTTG GCTGTGTCGG TGGCTATATC 
GCCAGCACTC GGGACTTGGT GGACATGGTG CGCTCCTACG CTGCAGGCTT CATCTTTACC 
ACTTCACTGC CTCCCATGGT GCTCTCTGGA GCTCTAGAGT CTGTGCGTCT ACTCAAGGGA 
GAGGAGGGTC AAGCCCTGAG GCGTGCACAC CAGCGCAACG TCAAACATAT GCGCCAGCTA 
CTAATGGACA GGGGCTTTCC TGTTATCCCC TGTCCCAGCC ACATCATCCC CATAAGGGTG 
GGTAATGCGG CACTCAACAG CAAGATCTGT GATCTTCTGC TCGCCAAGCA CAGCATCTAT 
GTGCAGGCCA TCAACTACCC AACTGTGCCT CGTGGTGAGG AGCTGCTGCG CTTGGCCCCC 
TCCCCCCACC ACAGCCCTCA GATGATGGAA AACTTTGTGG AGAAGTTGCT GCTGGCCTGG 
ACTGAGGTGG GGTTGCCCCT CCAAGATGTG TCTGTGGCTG CATGCAACTT CTGCCGTCGT 
CCTGTGCACT TTGAGCTTAT GAGCGAGTGG GAGCGATCCT ACTTTGGGAA CATGGGACCC 
CAATATGTTA CTACCTATGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037329.1
CDS	16..1779
Translation	MVAAAMLLRSCPVLSKGPTGLLGKVAKTYQFLFGIGRCPILATQGPTCSQIHLKATKAGADSPSWTKSHCPFMLSELQDRKSKIVQRAAPEVQEDVKTFKTDLLSFMESTTRSQSVPRFQDPEQTGGAPPLLIQNNMTGSQAFGYDQFFRDKIMEKKQDHTYRVFKTVNRWANAYPFAQHFSEASMDSKDVSVWCSNDYLGISRHPRVLQAIEETLKNHGAGAGGTRNISGTSKFHVELEQELAELHHKDSALLFSSCFVANDSTLFTLAKLLPGCEIYSDAGNHASMIQGIRNSGAVKFVFRHNDPGHLKKLLEKSDPKTPKIVAFETVHSMDGAICPLEELCDVAHQYGALTFVDEVHAVGLYGTRGAGIGERDGIMHKLDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGFPVIPCPSHIIPIRVGNAALNSKICDLLLAKHSIYVQAINYPTVPRGEELLRLAPSPHHSPQMMENFVEKLLLAWTEVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_013197.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5'-aminolevulinate synthase 2 (Alas2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013197.1

ORF Insert Sequence:

ATGGTGGCAG CAGCTATGTT GCTACGGTCC TGTCCAGTGC TCTCTAAGGG CCCCACAGGC 
CTCCTAGGCA AAGTGGCTAA AACTTACCAG TTCCTATTTG GTATTGGACG CTGCCCCATC 
CTGGCCACTC AAGGACCAAC CTGTTCTCAA ATCCATCTTA AGGCAACCAA GGCTGGAGCA 
GACTCTCCGT CTTGGACTAA GAGCCATTGT CCTTTCATGC TGTCAGAACT CCAAGACAGG 
AAGAGCAAGA TTGTGCAGAG GGCAGCTCCA GAAGTCCAAG AGGATGTCAA GACTTTCAAG 
ACAGACCTGC TGAGCTTCAT GGAGTCAACT ACCCGAAGCC AATCAGTGCC TCGTTTCCAG 
GATCCAGAGC AGACTGGAGG GGCACCTCCC CTGCTGATTC AGAATAATAT GACTGGAAGC 
CAGGCTTTTG GTTATGACCA ATTTTTTAGA GACAAGATCA TGGAAAAGAA GCAGGACCAC 
ACATACCGTG TGTTCAAGAC TGTGAATCGC TGGGCTAATG CCTACCCCTT TGCCCAACAC 
TTCTCCGAGG CATCTATGGA CTCAAAGGAT GTTTCTGTCT GGTGCAGTAA TGACTATTTG 
GGCATAAGCA GGCACCCTCG TGTCTTGCAG GCCATAGAGG AGACCCTGAA GAATCATGGA 
GCTGGAGCTG GGGGCACTCG CAATATCTCA GGTACAAGCA AGTTTCATGT GGAGCTTGAA 
CAGGAGCTGG CTGAACTACA TCATAAGGAC TCGGCTCTGC TCTTCTCCTC CTGTTTTGTG 
GCCAATGATT CTACTCTCTT TACACTGGCC AAGCTTCTGC CAGGGTGTGA GATCTACTCA 
GACGCAGGCA ATCATGCCTC CATGATCCAA GGCATTCGTA ACAGTGGTGC AGTCAAGTTT 
GTCTTCAGAC ACAATGACCC AGGCCACCTG AAGAAACTTC TTGAGAAGTC CGATCCCAAG 
ACACCAAAAA TTGTGGCTTT TGAGACTGTT CACTCCATGG ATGGTGCCAT CTGTCCTCTG 
GAGGAATTGT GTGATGTGGC CCACCAGTAC GGGGCCCTGA CCTTCGTGGA TGAAGTCCAT 
GCCGTAGGAC TATATGGAAC CCGGGGTGCA GGGATCGGGG AGCGTGATGG AATTATGCAC 
AAGCTTGACA TCATCTCTGG AACTCTTGGC AAGGCCTTTG GCTGTGTCGG TGGCTATATC 
GCCAGCACTC GGGACTTGGT GGACATGGTG CGCTCCTACG CTGCAGGCTT CATCTTTACC 
ACTTCACTGC CTCCCATGGT GCTCTCTGGA GCTCTAGAGT CTGTGCGTCT ACTCAAGGGA 
GAGGAGGGTC AAGCCCTGAG GCGTGCACAC CAGCGCAACG TCAAACATAT GCGCCAGCTA 
CTAATGGACA GGGGCTTTCC TGTTATCCCC TGTCCCAGCC ACATCATCCC CATAAGGGTG 
GGTAATGCGG CACTCAACAG CAAGATCTGT GATCTTCTGC TCGCCAAGCA CAGCATCTAT 
GTGCAGGCCA TCAACTACCC AACTGTGCCT CGTGGTGAGG AGCTGCTGCG CTTGGCCCCC 
TCCCCCCACC ACAGCCCTCA GATGATGGAA AACTTTGTGG AGAAGTTGCT GCTGGCCTGG 
ACTGAGGTGG GGTTGCCCCT CCAAGATGTG TCTGTGGCTG CATGCAACTT CTGCCGTCGT 
CCTGTGCACT TTGAGCTTAT GAGCGAGTGG GAGCGATCCT ACTTTGGGAA CATGGGACCC 
CAATATGTTA CTACCTATGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Synthesis, delivery and regulation of eukaryotic heme and Fe-S cluster cofactors.
Archives of biochemistry and biophysics59260-75(2016 Feb)
Barupala DP,Dzul SP,Riggs-Gelasco PJ,Stemmler TL

ALAS2 acts as a modifier gene in patients with congenital erythropoietic porphyria.
Blood118(6)1443-51(2011 Aug)
To-Figueras J,Ducamp S,Clayton J,Badenas C,Delaby C,Ged C,Lyoumi S,Gouya L,de Verneuil H,Beaumont C,Ferreira GC,Deybach JC,Herrero C,Puy H

A toxicogenomic approach for identifying biomarkers for myelosuppressive anemia in rats.
Toxicology282(3)139-45(2011 Apr)
Uehara T,Kondo C,Yamate J,Torii M,Maruyama T

New mutation in erythroid-specific delta-aminolevulinate synthase as the cause of X-linked sideroblastic anemia responsive to pyridoxine.
Acta haematologica125(4)193-7(2011)
Kucerova J,Horvathova M,Mojzikova R,Belohlavkova P,Cermak J,Divoky V

Fish oil feeding up-regulates the expression of 5-aminolevulinate synthase 2 mRNA in rat brain.
Bioscience, biotechnology, and biochemistry75(7)1383-5(2011)
Haraguchi T,Yanaka N,Eguchi Y,Kudo T,Hirata A,Kato N