Pcna cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcna
Entrez Gene ID	25737
Full Name	proliferating cell nuclear antigen
Synonyms	PCNAR,Pcna/cyclin
General protein information	Preferred Names proliferating cell nuclear antigen Names proliferating cell nuclear antigen cyclin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36
Summary	cell cycle protein that may contribute to the repair of DNA damage and to cell proliferation caused by exposure to pulmonary toxicants; may influence cell survival in ischaemically compromised tissue [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022381.3	NP_071776.1	proliferating cell nuclear antigen

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PCNA	NP_001029666.1
Mus musculus (house mouse)	Pcna	NP_035175.1
Rattus norvegicus (Norway rat)	Pcna	NP_071776.1
Drosophila melanogaster (fruit fly)	mus209	NP_995904.1
Caenorhabditis elegans (roundworm)	pcn-1	NP_500466.3
Schizosaccharomyces pombe (fission yeast)	pcn1	NP_596504.1
	PCNA	XP_001115746.1
Gallus gallus (chicken)	PCNA	NP_989501.1
Danio rerio (zebrafish)	pcna	XP_005155472.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010220	XP_319407.2
Arabidopsis thaliana (thale cress)	PCNA2	NP_180517.1
Canis lupus familiaris (dog)	PCNA	XP_534355.3
Saccharomyces cerevisiae (baker's yeast)	POL30	NP_009645.1
Arabidopsis thaliana (thale cress)	PCNA1	NP_172217.1
Pan troglodytes (chimpanzee)	PCNA	XP_001165515.1
Homo sapiens (human)	PCNA	NP_872590.1
Kluyveromyces lactis	KLLA0B12078g	XP_452067.1
Eremothecium gossypii	AGOS_ADL309W	NP_983787.2
Magnaporthe oryzae (rice blast fungus)	MGG_00518	XP_003718539.1
Neurospora crassa	NCU09239	XP_964674.1
Oryza sativa (rice)	Os02g0805200	NP_001048438.1
Xenopus tropicalis (tropical clawed frog)	pcna	NP_001007921.1

Gene Ontology
Bibliography

Related articles in PubMed

[Significance of the expression of proliferating cell nuclear antigen and P53 in the regeneration process of an atrophic parotid gland].
Yueduan W, Jinhua Z, Changling D, Yuhong Z, Lifang W, Bing S, Jing W
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology35(6)583-587(2017 Dec)

?-lipoic acid exerts neuroprotective effects on neuronal cells by upregulating the expression of PCNA via the P53 pathway in neurodegenerative conditions.
Li DW, Wang YD, Zhou SY, Sun WP
Molecular medicine reports14(5)4360-4366(2016 Nov)

Damage to dopaminergic neurons is mediated by proliferating cell nuclear antigen through the p53 pathway under conditions of oxidative stress in a cell model of Parkinson's disease.
Li DW, Li GR, Zhang BL, Feng JJ, Zhao H
International journal of molecular medicine37(2)429-35(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken together, these results indicated that ALA protected dopaminergic neurons against MPP+induced neurotoxicity through its ability to upregulate the DNA repair protein, PCNA, via the P53 pathway.
Title: ?-lipoic acid exerts neuroprotective effects on neuronal cells by upregulating the expression of PCNA via the P53 pathway in neurodegenerative conditions.

This indicates that MPP+-induced oxidative damage is mediated by the downregulation of PCNA through the p53 pathway in a cellular model of Parkinson's disease .
Title: Damage to dopaminergic neurons is mediated by proliferating cell nuclear antigen through the p53 pathway under conditions of oxidative stress in a cell model of Parkinson's disease.

gamma-TmT increased expression of PPARgamma and its downstream genes, PTEN and p27, and reduced PCNA expression in mammary tumors
Title: Dietary ?-Tocopherol-Rich Mixture Inhibits Estrogen-Induced Mammary Tumorigenesis by Modulating Estrogen Metabolism, Antioxidant Response, and PPAR?.

Dexamethasone induced placental and embryonal abnormalities which could be associated with reduced expressions of PCNA and cyclin D3, increased expressions of p27 and p57 and increased rate of apoptosis in IUGR placentas
Title: Determination of PCNA, cyclin D3, p27, p57 and apoptosis rate in normal and dexamethasone-induced intrauterine growth restricted rat placentas.

PCNA index decreased from 80 in HCC rats to 32 after boron treatment.
Title: Boron inhibits the proliferating cell nuclear antigen index, molybdenum containing proteins and ameliorates oxidative stress in hepatocellular carcinoma.

The following Pcna gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcna cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05686	NM_022381.3 Latest version!	Rattus norvegicus proliferating cell nuclear antigen (Pcna), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcna ( NM_022381.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071776.1 Rattus norvegicus proliferating cell nuclear antigen (Pcna), mRNA.

Price & Availability↑

CloneID	ORa05686
Clone ID Related Accession (Same CDS sequence)	NM_022381.3
Accession Version	NM_022381.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	proliferating cell nuclear antigen
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y00047.1. On Jun 10, 2007 this sequence version replaced NM_022381.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00047.1, FQ220101.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGTTTGAGG CACGCCTGAT CCAGGGCTCC ATCCTGAAGA AGGTGCTGGA GGCCCTCAAA 
GACCTCATCA ATGAGGCCTG CTGGGACATC AGTTCGGGCG GCGTGAACCT ACAGAGCATG 
GATTCGTCTC ACGTCTCCTT AGTGCAGCTT ACTCTGCGCT CCGAAGGCTT CGACACATAC 
CGCTGCGATC GCAACCTGGC CATGGGCGTG AACCTCACCA GCATGTCCAA AATTCTAAAA 
TGTGCTGGTA ATGAAGACAT CATTACATTA AGGGCTGAAG ATAATGCTGA TACCTTAGCA 
CTAGTATTTG AAGCACCAAA TCAAGAGAAA GTTTCAGACT ATGAGATGAA GTTAATGGAC 
TTAGACGTTG AGCAACTTGG AATCCCAGAA CAGGAGTACA GCTGCGTAGT AAAGATGCCA 
TCTGGTGAAT TTGCACGTAT ATGCCGGGAC CTTAGCCATA TTGGAGATGC TGTGGTGATC 
TCCTGTGCAA AGGACGGGGT GAAGTTTTCT GCGAGTGGGG AGCTTGGCAA TGGGAACATT 
AAGTTGTCCC AGACAAGCAA TGTTGATAAA GAAGAGGAAG CTGTGTCCAT AGAGATGAAT 
GAGCCAGTTC AGCTAACTTT TGCTCTGAGG TACCTGAACT TTTTCACAAA AGCCACTCCA 
CTGTCTCCTA CAGTAACACT CAGTATGTCT GCAGATGTAC CCCTTGTTGT AGAGTATAAA 
ATTGCTGACA TGGGACACTT AAAGTATTAT TTGGCTCCCA AGATCGAAGA TGAAGAAGGA 
TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071776.1
CDS	63..848
Translation	MFEARLIQGSILKKVLEALKDLINEACWDISSGGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVSIEMNEPVQLTFALRYLNFFTKATPLSPTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS

Target ORF information:

RefSeq Version	NM_022381.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proliferating cell nuclear antigen (Pcna), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022381.3

ORF Insert Sequence:

ATGTTTGAGG CACGCCTGAT CCAGGGCTCC ATCCTGAAGA AGGTGCTGGA GGCCCTCAAA 
GACCTCATCA ATGAGGCCTG CTGGGACATC AGTTCGGGCG GCGTGAACCT ACAGAGCATG 
GATTCGTCTC ACGTCTCCTT AGTGCAGCTT ACTCTGCGCT CCGAAGGCTT CGACACATAC 
CGCTGCGATC GCAACCTGGC CATGGGCGTG AACCTCACCA GCATGTCCAA AATTCTAAAA 
TGTGCTGGTA ATGAAGACAT CATTACATTA AGGGCTGAAG ATAATGCTGA TACCTTAGCA 
CTAGTATTTG AAGCACCAAA TCAAGAGAAA GTTTCAGACT ATGAGATGAA GTTAATGGAC 
TTAGACGTTG AGCAACTTGG AATCCCAGAA CAGGAGTACA GCTGCGTAGT AAAGATGCCA 
TCTGGTGAAT TTGCACGTAT ATGCCGGGAC CTTAGCCATA TTGGAGATGC TGTGGTGATC 
TCCTGTGCAA AGGACGGGGT GAAGTTTTCT GCGAGTGGGG AGCTTGGCAA TGGGAACATT 
AAGTTGTCCC AGACAAGCAA TGTTGATAAA GAAGAGGAAG CTGTGTCCAT AGAGATGAAT 
GAGCCAGTTC AGCTAACTTT TGCTCTGAGG TACCTGAACT TTTTCACAAA AGCCACTCCA 
CTGTCTCCTA CAGTAACACT CAGTATGTCT GCAGATGTAC CCCTTGTTGT AGAGTATAAA 
ATTGCTGACA TGGGACACTT AAAGTATTAT TTGGCTCCCA AGATCGAAGA TGAAGAAGGA 
TCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Significance of the expression of proliferating cell nuclear antigen and P53 in the regeneration process of an atrophic parotid gland].
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology35(6)583-587(2017 Dec)
Yueduan W,Jinhua Z,Changling D,Yuhong Z,Lifang W,Bing S,Jing W

?-lipoic acid exerts neuroprotective effects on neuronal cells by upregulating the expression of PCNA via the P53 pathway in neurodegenerative conditions.
Molecular medicine reports14(5)4360-4366(2016 Nov)
Li DW,Wang YD,Zhou SY,Sun WP

Damage to dopaminergic neurons is mediated by proliferating cell nuclear antigen through the p53 pathway under conditions of oxidative stress in a cell model of Parkinson's disease.
International journal of molecular medicine37(2)429-35(2016 Feb)
Li DW,Li GR,Zhang BL,Feng JJ,Zhao H

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Pcna cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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