Dbh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dbh
Entrez Gene ID	25699
Full Name	dopamine beta-hydroxylase
Synonyms	DOPBHY
General protein information	Preferred Names dopamine beta-hydroxylase Names dopamine beta-hydroxylase Dopamine beta hydroxylase (dopamine beta-monooxygenase) dopamine beta-monooxygenase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12
Summary	oxidoreductase that catalyzes the conversion of 3,4 dihydroxyphenethylamine, ascorbate, and O(2) to noradrenaline, dehydroascorbate, and H(2) in norephinephrine biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013158.2	NP_037290.2	dopamine beta-hydroxylase

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	dbh	XP_002942324.2
Canis lupus familiaris (dog)	DBH	NP_001005263.1
Bos taurus (cattle)	DBH	NP_851338.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010485	XP_307804.4
Pan troglodytes (chimpanzee)	DBH	XP_520341.2
Danio rerio (zebrafish)	dbh	NP_001103164.1
Homo sapiens (human)	DBH	NP_000778.3
	DBH	XP_001100196.2
Mus musculus (house mouse)	Dbh	NP_620392.2
Drosophila melanogaster (fruit fly)	Tbh	NP_788884.1
Rattus norvegicus (Norway rat)	Dbh	NP_037290.2
Gallus gallus (chicken)	DBH	XP_415429.4
Caenorhabditis elegans (roundworm)	tbh-1	NP_001257264.1

Gene Ontology
Bibliography

Related articles in PubMed

Age-related changes in immunoreactivity for dopamine ?-hydroxylase in carotid body glomus cells in spontaneously hypertensive rats.
Kato K, Fushuku S, Yamamoto Y
Autonomic neuroscience : basic & clinical20550-56(2017 Jul)

Gene expression and content of enzymes of noradrenaline synthesis in the rat organ of Zuckerkandl at the critical period of morphogenesis.
Murtazina AR, Nikishina YO, Bondarenko NS, Sapronova AY, Volina EV, Ugrumov MV
Doklady. Biochemistry and biophysics474(1)200-203(2017 May)

Effects of transgenic expression of dopamine beta hydroxylase (Dbh) gene on blood pressure in spontaneously hypertensive rats.
Pravenec M, Landa V, Z?dek V, Mlejnek P, ?ilhav? J, Mir SA, Vaingankar SM, Wang J, Kurtz TW
Physiological research65(6)1039-1044(2016 Dec)

The crystal structure of human dopamine ?-hydroxylase at 2.9 ? resolution.
Vendelboe TV, Harris P, Zhao Y, Walter TS, Harlos K, El Omari K, Christensen HE
Science advances2(4)e1500980(2016 Apr)

Human Bacterial Artificial Chromosome (BAC) Transgenesis Fully Rescues Noradrenergic Function in Dopamine ?-Hydroxylase Knockout Mice.
Cubells JF, Schroeder JP, Barrie ES, Manvich DF, Sadee W, Berg T, Mercer K, Stowe TA, Liles LC, Squires KE, Mezher A, Curtin P, Perdomo DL, Szot P, Weinshenker D
PloS one11(5)e0154864(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study revealed age-related changes in tyrosine hydroxylase and DBH immunoreactivities in the carotid body glomus cells of spontaneously hypertensive (SHR/Izm) and Wistar Kyoto (WKY/Izm) rats at 4, 8, 12, and 16 weeks of age using immunohistochemistry. DBH age-dependently increased in the glomus cells of SHR/Izm in contrast to WKY/Izm.
Title: Age-related changes in immunoreactivity for dopamine ?-hydroxylase in carotid body glomus cells in spontaneously hypertensive rats.

tyrosine hydroxylase and dopamine beta-hydroxylase-was evaluated in the organ of Zuckerkandl of rats in the critical period of morphogenesis
Title: Gene expression and content of enzymes of noradrenaline synthesis in the rat organ of Zuckerkandl at the critical period of morphogenesis.

genetically determined decreases in adrenal expression and activity of DBH do not represent primary determinants of increased blood pressure in the SHR model
Title: Effects of transgenic expression of dopamine beta hydroxylase (Dbh) gene on blood pressure in spontaneously hypertensive rats.

Data suggest that the number of glomus cells with strong DBH immunoreactivity is increased in carotid bodies in spontaneously hypertensive rats compared with control rats; DBH is mainly observed in nerve fibers around blood vessels in control rats.
Title: Increased total volume and dopamine ?-hydroxylase immunoreactivity of carotid body in spontaneously hypertensive rats.

With social isolation, no changes were observed in protein levels of DBH and NET in the right hippocampus, whereas expression of this norepinephrine synthetizing enzyme and transporter were elevated in the left hippocampus.
Title: Hippocampal asymmetry in expression of catecholamine synthesizing enzyme and transporters in socially isolated rats.

The following Dbh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dbh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02154	NM_013158.2 Latest version!	Rattus norvegicus dopamine beta-hydroxylase (Dbh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dbh ( NM_013158.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037290.2 Rattus norvegicus dopamine beta-hydroxylase (Dbh), mRNA.

Price & Availability↑

CloneID	ORa02154
Clone ID Related Accession (Same CDS sequence)	NM_013158.2
Accession Version	NM_013158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	dopamine beta-hydroxylase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from L12407.1 and BF525224.1. On Apr 5, 2007 this sequence version replaced NM_013158.1. Sequence Note: This sequence has been modified as follows: removed 34 bp suspected to be vector contamination from the 3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02642595, SAMN02642597 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCCTC ACCTCAGCCA CCAGCCTTGC TGGAGCCTCC CCAGCCCCAG CGTCCGTGAG 
GCGGCTTCCA TGTATGGCAC TGCTGTGGCC ATCTTCCTGG TCATCCTGGT GGCTGCACTG 
CAGGGCTCGG AGCCTCCGGA GAGCCCCTTC CCTTACCACA TCCCCCTGGA CCCTGAAGGG 
ACTTTAGAGC TCTCGTGGAA CGTCAGCTAT GACCAGGAGA TCATCCACTT CCAGCTCCAG 
GTGCAAGGGC CGAGGGCTGG GGTCCTGTTC GGAATGTCGG ATCGAGGTGA GATGGAGAAC 
GCAGACCTCG TCATGCTCTG GACTGACGGG GACAGGACCT ACTTTGCGGA CGCCTGGAGT 
GACCAGAAAG GGCAGATCCA TCTGGATACC CATCAGGACT ACCAGCTGCT CCAGGCACAG 
AGGGTGTCAA ACAGCCTATC CCTGCTCTTC AAGAGGCCCT TTGTCACCTG CGACCCCAAG 
GATTATGTCA TTGAGGATGA CACTGTCCAT CTAGTGTATG GGATCCTGGA GGAGCCATTC 
CAGTCCCTGG AGGCCATCAA CACCTCAGGC CTACATACGG GACTACAGCA GGTGCAGCTT 
CTGAAGCCTG AGGTCTCCAC TCCAGCCATG CCTGCGGATG TACAAACCAT GGATATCCGG 
GCTCCTGACG TCCTCATCCC CAGCACTGAG ACCACATACT GGTGCTATAT CACTGAGCTA 
CCCCTACACT TCCCCCGACA CCACATCATC ATGTATGAGG CCATTGTCAC GGAGGGCAAT 
GAGGCCCTGG TGCACCACAT GGAGGTCTTC CAATGTACAA ATGAGTCTGA GGCCTTCCCC 
ATGTTCAACG GACCCTGCGA CTCCAAGATG AAACCTGACA GACTCAACTA CTGTCGCCAC 
GTGCTGGCGG CATGGGCCCT GGGCGCCAAG GCATTTTACT ACCCAGAAGA AGCCGGTGTC 
CCTTTGGGGA GCTCAGGATC CTCCCGGTTT CTCCGACTGG AAGTTCATTA CCACAATCCA 
CGGAATATAC AAGGCCGGCG CGACTCCTCT GGCATCCGTC TACACTACAC AGCTAGTCTC 
CGACCCAATG AGGCAGGCAT CATGGAGCTT GGACTGGTGT ACACGCCCTT GATGGCCATC 
CCCCCTCAGG AGACCACCTT TGTTTTGACT GGCTACTGCA CAGACAGGTG CACCCAGATG 
GCACTGCCGA AATCTGGAAT CCGCATCTTT GCCTCACAGC TCCACACGCA CCTGACCGGC 
AGGAAGGTGA TTACTGTGCT CGCCAGGGAT GGCCAACAGA GGGAAGTGGT GAACAGAGAC 
AACCACTACA GCCCCCACTT TCAGGAGATC AGAATGCTGA AGAATGCTGT GACTGTCCAC 
CAGGGGGATG TCCTCATCAC TTCGTGCACA TACAACACAG AAAACAGGAC AATGGCCACA 
GTGGGGGGGT TTGGAATCTT GGAGGAGATG TGCGTCAACT ACGTCCACTA CTACCCCAAA 
ACAGAGCTGG AGCTCTGCAA GAGTGCCGTG GATGATGGCT TCCTGCAGAA ATACTTCCAC 
ATAGTAAACC GGTTCGGCAA TGAGGAGGTC TGCACCTGCC CTCAGGCCTC TGTCCCCCAG 
CAGTTCGCCT CTGTGCCCTG GAACTCTTTC AATCGTGATA TGCTCAAGGC TTTGTATAAC 
TATGCCCCTA TCTCTGTGCA CTGTAACAAG ACCTCTGCCG TCCGCTTCCC GGGTAACTGG 
AACCTGCAGC CTCTGCCTAA GATCACTTCC GCAGTGGAAG AACCCGACCC ACGCTGCCCC 
ATCCGACAGA CTCGGGGACC CGCCGGCCCC TTCGTTGTCA TCACCACGGA GGCAGACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037290.2
CDS	7..1872
Translation	MQPHLSHQPCWSLPSPSVREAASMYGTAVAIFLVILVAALQGSEPPESPFPYHIPLDPEGTLELSWNVSYDQEIIHFQLQVQGPRAGVLFGMSDRGEMENADLVMLWTDGDRTYFADAWSDQKGQIHLDTHQDYQLLQAQRVSNSLSLLFKRPFVTCDPKDYVIEDDTVHLVYGILEEPFQSLEAINTSGLHTGLQQVQLLKPEVSTPAMPADVQTMDIRAPDVLIPSTETTYWCYITELPLHFPRHHIIMYEAIVTEGNEALVHHMEVFQCTNESEAFPMFNGPCDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGVPLGSSGSSRFLRLEVHYHNPRNIQGRRDSSGIRLHYTASLRPNEAGIMELGLVYTPLMAIPPQETTFVLTGYCTDRCTQMALPKSGIRIFASQLHTHLTGRKVITVLARDGQQREVVNRDNHYSPHFQEIRMLKNAVTVHQGDVLITSCTYNTENRTMATVGGFGILEEMCVNYVHYYPKTELELCKSAVDDGFLQKYFHIVNRFGNEEVCTCPQASVPQQFASVPWNSFNRDMLKALYNYAPISVHCNKTSAVRFPGNWNLQPLPKITSAVEEPDPRCPIRQTRGPAGPFVVITTEADTE

Target ORF information:

RefSeq Version	NM_013158.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dopamine beta-hydroxylase (Dbh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013158.2

ORF Insert Sequence:

ATGCAGCCTC ACCTCAGCCA CCAGCCTTGC TGGAGCCTCC CCAGCCCCAG CGTCCGTGAG 
GCGGCTTCCA TGTATGGCAC TGCTGTGGCC ATCTTCCTGG TCATCCTGGT GGCTGCACTG 
CAGGGCTCGG AGCCTCCGGA GAGCCCCTTC CCTTACCACA TCCCCCTGGA CCCTGAAGGG 
ACTTTAGAGC TCTCGTGGAA CGTCAGCTAT GACCAGGAGA TCATCCACTT CCAGCTCCAG 
GTGCAAGGGC CGAGGGCTGG GGTCCTGTTC GGAATGTCGG ATCGAGGTGA GATGGAGAAC 
GCAGACCTCG TCATGCTCTG GACTGACGGG GACAGGACCT ACTTTGCGGA CGCCTGGAGT 
GACCAGAAAG GGCAGATCCA TCTGGATACC CATCAGGACT ACCAGCTGCT CCAGGCACAG 
AGGGTGTCAA ACAGCCTATC CCTGCTCTTC AAGAGGCCCT TTGTCACCTG CGACCCCAAG 
GATTATGTCA TTGAGGATGA CACTGTCCAT CTAGTGTATG GGATCCTGGA GGAGCCATTC 
CAGTCCCTGG AGGCCATCAA CACCTCAGGC CTACATACGG GACTACAGCA GGTGCAGCTT 
CTGAAGCCTG AGGTCTCCAC TCCAGCCATG CCTGCGGATG TACAAACCAT GGATATCCGG 
GCTCCTGACG TCCTCATCCC CAGCACTGAG ACCACATACT GGTGCTATAT CACTGAGCTA 
CCCCTACACT TCCCCCGACA CCACATCATC ATGTATGAGG CCATTGTCAC GGAGGGCAAT 
GAGGCCCTGG TGCACCACAT GGAGGTCTTC CAATGTACAA ATGAGTCTGA GGCCTTCCCC 
ATGTTCAACG GACCCTGCGA CTCCAAGATG AAACCTGACA GACTCAACTA CTGTCGCCAC 
GTGCTGGCGG CATGGGCCCT GGGCGCCAAG GCATTTTACT ACCCAGAAGA AGCCGGTGTC 
CCTTTGGGGA GCTCAGGATC CTCCCGGTTT CTCCGACTGG AAGTTCATTA CCACAATCCA 
CGGAATATAC AAGGCCGGCG CGACTCCTCT GGCATCCGTC TACACTACAC AGCTAGTCTC 
CGACCCAATG AGGCAGGCAT CATGGAGCTT GGACTGGTGT ACACGCCCTT GATGGCCATC 
CCCCCTCAGG AGACCACCTT TGTTTTGACT GGCTACTGCA CAGACAGGTG CACCCAGATG 
GCACTGCCGA AATCTGGAAT CCGCATCTTT GCCTCACAGC TCCACACGCA CCTGACCGGC 
AGGAAGGTGA TTACTGTGCT CGCCAGGGAT GGCCAACAGA GGGAAGTGGT GAACAGAGAC 
AACCACTACA GCCCCCACTT TCAGGAGATC AGAATGCTGA AGAATGCTGT GACTGTCCAC 
CAGGGGGATG TCCTCATCAC TTCGTGCACA TACAACACAG AAAACAGGAC AATGGCCACA 
GTGGGGGGGT TTGGAATCTT GGAGGAGATG TGCGTCAACT ACGTCCACTA CTACCCCAAA 
ACAGAGCTGG AGCTCTGCAA GAGTGCCGTG GATGATGGCT TCCTGCAGAA ATACTTCCAC 
ATAGTAAACC GGTTCGGCAA TGAGGAGGTC TGCACCTGCC CTCAGGCCTC TGTCCCCCAG 
CAGTTCGCCT CTGTGCCCTG GAACTCTTTC AATCGTGATA TGCTCAAGGC TTTGTATAAC 
TATGCCCCTA TCTCTGTGCA CTGTAACAAG ACCTCTGCCG TCCGCTTCCC GGGTAACTGG 
AACCTGCAGC CTCTGCCTAA GATCACTTCC GCAGTGGAAG AACCCGACCC ACGCTGCCCC 
ATCCGACAGA CTCGGGGACC CGCCGGCCCC TTCGTTGTCA TCACCACGGA GGCAGACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Age-related changes in immunoreactivity for dopamine ?-hydroxylase in carotid body glomus cells in spontaneously hypertensive rats.
Autonomic neuroscience : basic & clinical20550-56(2017 Jul)
Kato K,Fushuku S,Yamamoto Y

Gene expression and content of enzymes of noradrenaline synthesis in the rat organ of Zuckerkandl at the critical period of morphogenesis.
Doklady. Biochemistry and biophysics474(1)200-203(2017 May)
Murtazina AR,Nikishina YO,Bondarenko NS,Sapronova AY,Volina EV,Ugrumov MV

Effects of transgenic expression of dopamine beta hydroxylase (Dbh) gene on blood pressure in spontaneously hypertensive rats.
Physiological research65(6)1039-1044(2016 Dec)
Pravenec M,Landa V,Z?dek V,Mlejnek P,?ilhav? J,Mir SA,Vaingankar SM,Wang J,Kurtz TW

The crystal structure of human dopamine ?-hydroxylase at 2.9 ? resolution.
Science advances2(4)e1500980(2016 Apr)
Vendelboe TV,Harris P,Zhao Y,Walter TS,Harlos K,El Omari K,Christensen HE

Human Bacterial Artificial Chromosome (BAC) Transgenesis Fully Rescues Noradrenergic Function in Dopamine ?-Hydroxylase Knockout Mice.
PloS one11(5)e0154864(2016)
Cubells JF,Schroeder JP,Barrie ES,Manvich DF,Sadee W,Berg T,Mercer K,Stowe TA,Liles LC,Squires KE,Mezher A,Curtin P,Perdomo DL,Szot P,Weinshenker D

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Dbh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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