Ctsl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctsl
Entrez Gene ID	25697
Full Name	cathepsin L
Synonyms	CATHL,CatL,Ctsl1
General protein information	Preferred Names cathepsin L Names cathepsin L1 CP-2 MEP cyclic protein 2 major excreted protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17p14
Summary	mouse homolog is deleted in the CD4 T-cell-deficient mouse mutant nacht (nkt); may play a role in CD4+ T-cell selection in the thymus [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013156.2	NP_037288.1	cathepsin L1 preproprotein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT5G43060	NP_568620.1
Canis lupus familiaris (dog)	CTSL	NP_001239115.1
Bos taurus (cattle)	CTSV	NP_776457.1
Mus musculus (house mouse)	Ctsl	NP_034114.1
Caenorhabditis elegans (roundworm)	cpl-1	NP_001256718.1
Arabidopsis thaliana (thale cress)	AT3G19390	NP_566633.1
Xenopus tropicalis (tropical clawed frog)	ctsv	NP_001004869.1
Danio rerio (zebrafish)	ctsla	NP_997749.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011828	XP_001689282.1
Arabidopsis thaliana (thale cress)	RD21	NP_564497.1
Oryza sativa (rice)	Os04g0670500	NP_001054213.1
Oryza sativa (rice)	Os04g0670200	NP_001054211.2
Pan troglodytes (chimpanzee)	CTSL2	XP_520130.2
	CTSL2	XP_002800251.1
Homo sapiens (human)	CTSV	NP_001324.2
Canis lupus familiaris (dog)	LOC102156614	XP_005615836.1
Rattus norvegicus (Norway rat)	Ctsl1	NP_037288.1
Gallus gallus (chicken)	CTSL2	NP_001161481.1
Drosophila melanogaster (fruit fly)	Cp1	NP_523735.2
Arabidopsis thaliana (thale cress)	AT3G19400	NP_566634.2
Arabidopsis thaliana (thale cress)	AT3G43960	NP_566867.1

Gene Ontology
Bibliography

Related articles in PubMed

Lysosomal processing of progranulin.
Zhou X, Paushter DH, Feng T, Sun L, Reinheckel T, Hu F
Molecular neurodegeneration12(1)62(2017 08)

The lysosomal protein cathepsin L is a progranulin protease.
Lee CW, Stankowski JN, Chew J, Cook CN, Lam YW, Almeida S, Carlomagno Y, Lau KF, Prudencio M, Gao FB, Bogyo M, Dickson DW, Petrucelli L
Molecular neurodegeneration12(1)55(2017 07)

Sub-lethal oxidative stress induces lysosome biogenesis via a lysosomal membrane permeabilization-cathepsin-caspase 3-transcription factor EB-dependent pathway.
Leow SM, Chua SX, Venkatachalam G, Shen L, Luo L, Clement MV
Oncotarget8(10)16170-16189(2017 Mar)

Activation of cathepsin L contributes to the irreversible depolarization induced by oxygen and glucose deprivation in rat hippocampal CA1 neurons.
Kikuta S, Murai Y, Tanaka E
Neuroscience letters636120-126(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate the role of a cathepsin-caspase 3 axis in the nuclear translocation of transcription Factor EB (TFEB) leading to lysosome biogenesis under conditions of sub-lethal oxidative stress.
Title: Sub-lethal oxidative stress induces lysosome biogenesis via a lysosomal membrane permeabilization-cathepsin-caspase 3-transcription factor EB-dependent pathway.

the activation of cathepsin L contributes to the irreversible depolarization produced by oxygen and glucose deprivation
Title: Activation of cathepsin L contributes to the irreversible depolarization induced by oxygen and glucose deprivation in rat hippocampal CA1 neurons.

Cathepsins in Rotator Cuff Tendinopathy: Identification in Human Chronic Tears and Temporal Induction in a Rat Model.
Title: Cathepsins in Rotator Cuff Tendinopathy: Identification in Human Chronic Tears and Temporal Induction in a Rat Model.

Inhibition of the activation of cathepsin L contributes to neuroprotection against cerebral ischemia.
Title: Inhibition of cysteine cathepsin B and L activation in astrocytes contributes to neuroprotection against cerebral ischemia via blocking the tBid-mitochondrial apoptotic signaling pathway.

Changes in olfaction were less consistent and widespread in the hippocampus, but were again sex specific for three genes: cathepsin-L (CtsL), matrix metalloproteinase-14 (MMP-14) and MMP-16.
Title: Novel odors affect gene expression for cytokines and proteinases in the rat amygdala and hippocampus.

The following Ctsl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctsl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13776	NM_013156.2 Latest version!	Rattus norvegicus cathepsin L (Ctsl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctsl ( NM_013156.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037288.1 Rattus norvegicus cathepsin L (Ctsl), mRNA.

Price & Availability↑

CloneID	ORa13776
Clone ID Related Accession (Same CDS sequence)	NM_013156.2
Accession Version	NM_013156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	cathepsin L1 preproprotein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Y00697.1. On Aug 30, 2012 this sequence version replaced NM_013156.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00697.1, FQ218649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906352, SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGACCCCTT TACTCCTCCT GGCTGTCCTC TGCTTGGGAA CAGCCTTAGC CACTCCAAAA 
TTTGATCAAA CGTTTAATGC ACAGTGGCAC CAGTGGAAGT CCACACACAG AAGACTGTAT 
GGCACGAATG AGGAAGAGTG GAGGAGAGCA GTGTGGGAGA AGAACATGAG GATGATCCAG 
CTACACAATG GGGAGTACAG CAACGGGAAG CACGGCTTTA CCATGGAGAT GAACGCCTTC 
GGTGACATGA CCAATGAGGA ATTCAGGCAG ATAGTGAATG GCTATCGCCA CCAGAAGCAC 
AAGAAGGGAA GGTTATTTCA GGAACCTCTG ATGCTGCAGA TCCCCAAGAC TGTGGACTGG 
AGAGAAAAGG GTTGTGTGAC TCCTGTGAAG AATCAGGGCC AGTGTGGTTC TTGCTGGGCT 
TTTAGCGCAT CGGGTTGCCT AGAAGGACAG ATGTTCCTTA AGACTGGCAA ACTGATCTCA 
CTGAGTGAAC AGAACCTTGT GGACTGTTCT CACGATCAAG GCAATCAGGG CTGTAATGGA 
GGCCTGATGG ATTTTGCTTT CCAGTACATT AAGGAAAATG GAGGTCTGGA CTCAGAGGAG 
TCTTATCCCT ATGAAGCAAA GGATGGATCT TGTAAATACA GAGCTGAGTA TGCTGTGGCT 
AACGACACAG GGTTTGTGGA TATCCCTCAG CAAGAGAAAG CCCTCATGAA GCCTGTAGCG 
ACGGTGGGGC CTATTTCTGT TGCCATGGAT GCAAGCCATC CGTCTCTCCA GTTCTATAGT 
TCAGGTATCT ACTATGAACC CAACTGTAGC AGCAAGGACC TCGACCATGG GGTTCTGGTG 
GTTGGCTATG GTTATGAAGG AACAGATTCA AATAAGGATA AATACTGGCT TGTCAAAAAC 
AGCTGGGGTA AAGAATGGGG TATGGATGGC TACATCAAAA TAGCCAAAGA CCGGAACAAC 
CACTGCGGAC TTGCCACCGC AGCCAGCTAT CCTATCGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037288.1
CDS	74..1078
Translation	MTPLLLLAVLCLGTALATPKFDQTFNAQWHQWKSTHRRLYGTNEEEWRRAVWEKNMRMIQLHNGEYSNGKHGFTMEMNAFGDMTNEEFRQIVNGYRHQKHKKGRLFQEPLMLQIPKTVDWREKGCVTPVKNQGQCGSCWAFSASGCLEGQMFLKTGKLISLSEQNLVDCSHDQGNQGCNGGLMDFAFQYIKENGGLDSEESYPYEAKDGSCKYRAEYAVANDTGFVDIPQQEKALMKPVATVGPISVAMDASHPSLQFYSSGIYYEPNCSSKDLDHGVLVVGYGYEGTDSNKDKYWLVKNSWGKEWGMDGYIKIAKDRNNHCGLATAASYPIVN

Target ORF information:

RefSeq Version	NM_013156.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cathepsin L (Ctsl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013156.2

ORF Insert Sequence:

ATGACCCCTT TACTCCTCCT GGCTGTCCTC TGCTTGGGAA CAGCCTTAGC CACTCCAAAA 
TTTGATCAAA CGTTTAATGC ACAGTGGCAC CAGTGGAAGT CCACACACAG AAGACTGTAT 
GGCACGAATG AGGAAGAGTG GAGGAGAGCA GTGTGGGAGA AGAACATGAG GATGATCCAG 
CTACACAATG GGGAGTACAG CAACGGGAAG CACGGCTTTA CCATGGAGAT GAACGCCTTC 
GGTGACATGA CCAATGAGGA ATTCAGGCAG ATAGTGAATG GCTATCGCCA CCAGAAGCAC 
AAGAAGGGAA GGTTATTTCA GGAACCTCTG ATGCTGCAGA TCCCCAAGAC TGTGGACTGG 
AGAGAAAAGG GTTGTGTGAC TCCTGTGAAG AATCAGGGCC AGTGTGGTTC TTGCTGGGCT 
TTTAGCGCAT CGGGTTGCCT AGAAGGACAG ATGTTCCTTA AGACTGGCAA ACTGATCTCA 
CTGAGTGAAC AGAACCTTGT GGACTGTTCT CACGATCAAG GCAATCAGGG CTGTAATGGA 
GGCCTGATGG ATTTTGCTTT CCAGTACATT AAGGAAAATG GAGGTCTGGA CTCAGAGGAG 
TCTTATCCCT ATGAAGCAAA GGATGGATCT TGTAAATACA GAGCTGAGTA TGCTGTGGCT 
AACGACACAG GGTTTGTGGA TATCCCTCAG CAAGAGAAAG CCCTCATGAA GCCTGTAGCG 
ACGGTGGGGC CTATTTCTGT TGCCATGGAT GCAAGCCATC CGTCTCTCCA GTTCTATAGT 
TCAGGTATCT ACTATGAACC CAACTGTAGC AGCAAGGACC TCGACCATGG GGTTCTGGTG 
GTTGGCTATG GTTATGAAGG AACAGATTCA AATAAGGATA AATACTGGCT TGTCAAAAAC 
AGCTGGGGTA AAGAATGGGG TATGGATGGC TACATCAAAA TAGCCAAAGA CCGGAACAAC 
CACTGCGGAC TTGCCACCGC AGCCAGCTAT CCTATCGTGA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lysosomal processing of progranulin.
Molecular neurodegeneration12(1)62(2017 08)
Zhou X,Paushter DH,Feng T,Sun L,Reinheckel T,Hu F

The lysosomal protein cathepsin L is a progranulin protease.
Molecular neurodegeneration12(1)55(2017 07)
Lee CW,Stankowski JN,Chew J,Cook CN,Lam YW,Almeida S,Carlomagno Y,Lau KF,Prudencio M,Gao FB,Bogyo M,Dickson DW,Petrucelli L

Sub-lethal oxidative stress induces lysosome biogenesis via a lysosomal membrane permeabilization-cathepsin-caspase 3-transcription factor EB-dependent pathway.
Oncotarget8(10)16170-16189(2017 Mar)
Leow SM,Chua SX,Venkatachalam G,Shen L,Luo L,Clement MV

Activation of cathepsin L contributes to the irreversible depolarization induced by oxygen and glucose deprivation in rat hippocampal CA1 neurons.
Neuroscience letters636120-126(2017 Jan)
Kikuta S,Murai Y,Tanaka E

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Ctsl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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