gRNAs
The following Clcn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clcn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa02126 | |
| Clone ID Related Accession (Same CDS sequence) | NM_013147.1 | |
| Accession Version | NM_013147.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-11-15 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | chloride channel protein 1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X62894.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X62894.1, AY112740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689600, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGCGGT CCCAGTCCCA GCAACATGGA GGTGAACAAA GCTGGTGGGG CACTGCCCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_037279.1 |
| CDS | 84..3068 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_013147.1 |
1 | ATGGAGCGGT CCCAGTCCCA GCAACATGGA GGTGAACAAA GCTGGTGGGG CACTGCCCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa43200 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008762873.2 | |
| Accession Version | XM_008762873.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | chloride channel protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCGGT CCCAGTCCCA GCAACATGGA GGTGAACAAA GCTGGTGGGG CACTGCCCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008761095.1 |
| CDS | 1249..4131 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008762873.2 |
1 | ATGGAGCGGT CCCAGTCCCA GCAACATGGA GGTGAACAAA GCTGGTGGGG CACTGCCCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Impaired surface membrane insertion of homo- and heterodimeric human muscle chloride channels carrying amino-terminal myotonia-causing mutations. |
Clinical, Molecular, and Functional Characterization of CLCN1 Mutations in Three Families with Recessive Myotonia Congenita. |
Myotonia congenita: novel mutations in CLCN1 gene and functional characterizations in Italian patients. |
Regulation of ClC-1 and KATP channels in action potential-firing fast-twitch muscle fibers. |
Statins and fenofibrate affect skeletal muscle chloride conductance in rats by differently impairing ClC-1 channel regulation and expression. |
