Gnai1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnai1
Entrez Gene ID	25686
Full Name	G protein subunit alpha i1
Synonyms	BPGTPB
General protein information	Preferred Names G protein subunit alpha i1 Names guanine nucleotide-binding protein G(i) subunit alpha-1 adenylate cyclase-inhibiting G alpha protein guanine nucleotide binding protein (G protein), alpha inhibiting 1 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 guanine nucleotide binding protein, alpha inhibiting 1 guanine nucleotide-binding protein G(i), alpha-1 subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q11
Summary	plays a role in presynaptic inhibition and regulation of synaptic transmission [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013145.1	NP_037277.1	guanine nucleotide-binding protein G(i) subunit alpha-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAI1	XP_001159548.1
Danio rerio (zebrafish)	gnai1	NP_957265.1
Rattus norvegicus (Norway rat)	Gnai1	NP_037277.1
Mus musculus (house mouse)	Gnai1	NP_034435.1
Gallus gallus (chicken)	GNAI1	NP_990734.1
Drosophila melanogaster (fruit fly)	G-ialpha65A	NP_477502.1
Xenopus tropicalis (tropical clawed frog)	gnai1	NP_001090865.1
Homo sapiens (human)	GNAI1	NP_002060.4
Bos taurus (cattle)	GNAI1	NP_776749.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005904	XP_315935.4
Caenorhabditis elegans (roundworm)	gpa-16	NP_490790.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide-free state of heterotrimeric G proteins ?-subunit adopts a highly stable conformation.
Andhirka SK, Vignesh R, Aradhyam GK
The FEBS journal284(15)2464-2481(2017 Aug)

Effect of N-Terminal Myristoylation on the Active Conformation of G?
van Keulen SC, Rothlisberger U
Biochemistry56(1)271-280(2017 Jan)

Nanosecond Dynamics of G?i1 Bound to Nucleotides or Ric-8A, a G? Chaperone with GEF Activity.
Black LA, Thomas CJ, Nix GN, Terwilliger MC, Sprang SR, Ross JBA
Biophysical journal111(4)722-731(2016 Aug)

SAPCD2 Controls Spindle Orientation and Asymmetric Divisions by Negatively Regulating the G?i-LGN-NuMA Ternary Complex.
Chiu CWN, Monat C, Robitaille M, Lacomme M, Daulat AM, Macleod G, McNeill H, Cayouette M, Angers S
Developmental cell36(1)50-62(2016 Jan)

The Orphan G Protein-coupled Receptor GPR17 Negatively Regulates Oligodendrocyte Differentiation via G?i/o and Its Downstream Effector Molecules.
Simon K, Hennen S, Merten N, Bl?ttermann S, Gillard M, Kostenis E, Gomeza J
The Journal of biological chemistry291(2)705-18(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Gnai1 subunit of GTP-binding protein catalyzes nucleotide exchange by selective perturbance of GDP-bound conformation of Gnai1 to metastable intermediate structural state and accelerates rate-limiting step of GDP release by surpassing kinetic barrier.
Title: The nucleotide-free state of heterotrimeric G proteins ?-subunit adopts a highly stable conformation.

Molecular dynamics simulation of a myristoylated G protein subunit alpha i1 (Galphai1)-GTP complex suggests that the myristoyl-induced conformational changes of the switch II and alpha helical domains create new possibilities for protein-protein interactions.
Title: Effect of N-Terminal Myristoylation on the Active Conformation of G?

Induction of Galphai1 structural heterogeneity by Ric-8A provides a mechanism for nucleotide release
Title: Nanosecond Dynamics of G?i1 Bound to Nucleotides or Ric-8A, a G? Chaperone with GEF Activity.

stimulation of GPR17 by the small molecule agonist MDL29,951 (2-carboxy-4,6-dichloro-1H-indole-3-propionic acid) decreases myelin basic protein expression levels mainly by triggering the Galphai/o signaling pathway.
Title: The Orphan G Protein-coupled Receptor GPR17 Negatively Regulates Oligodendrocyte Differentiation via G?i/o and Its Downstream Effector Molecules.

These results in part explain the mobility of Galphai1 protein upon reversible palmitoylation to meet one or another type of signaling protein partner.
Title: G protein-membrane interactions I: G?i1 myristoyl and palmitoyl modifications in protein-lipid interactions and its implications in membrane microdomain localization.

The following Gnai1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnai1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00044	NM_013145.1 Latest version!	Rattus norvegicus G protein subunit alpha i1 (Gnai1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnai1 ( NM_013145.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037277.1 Rattus norvegicus G protein subunit alpha i1 (Gnai1), mRNA.

Price & Availability↑

CloneID	ORa00044
Clone ID Related Accession (Same CDS sequence)	NM_013145.1
Accession Version	NM_013145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(i) subunit alpha-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M17527.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M17527.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCTGCA CACTGAGCGC TGAGGACAAG GCGGCCGTGG AGCGCAGCAA GATGATCGAC 
CGCAACCTCC GGGAGGACGG AGAGAAGGCA GCGCGCGAGG TCAAGCTGCT GCTGCTGGGT 
GCTGGTGAAT CCGGGAAGAG CACAATTGTG AAGCAGATGA AAATTATCCA CGAGGCTGGC 
TACTCAGAGG AAGAGTGTAA GCAGTACAAA GCAGTGGTCT ACAGCAACAC CATCCAGTCC 
ATCATTGCCA TCATTAGAGC CATGGGGAGA TTGAAAATCG ACTTTGGAGA CGCTGCTCGT 
GCGGATGATG CTCGCCAACT CTTCGTGCTT GCTGGGGCTG CAGAGGAAGG CTTTATGACC 
GCGGAGCTCG CCGGCGTCAT AAAGAGACTG TGGAAGGACA GCGGTGTGCA AGCCTGCTTC 
AACAGATCCC GGGAGTACCA GCTGAACGAT TCGGCGGCGT ACTACCTGAA TGACTTGGAC 
AGAATAGCAC AACCAAATTA CATCCCAACC CAGCAGGATG TTCTCAGAAC TAGAGTGAAA 
ACGACGGGAA TTGTGGAAAC CCACTTTACT TTCAAAGATC TTCATTTTAA AATGTTTGAC 
GTGGGAGGCC AGAGATCAGA GCGGAAGAAG TGGATTCACT GCTTTGAAGG CGTGACTGCC 
ATCATCTTCT GTGTGGCCCT GAGTGACTAT GACCTGGTTC TTGCTGAGGA TGAAGAAATG 
AACCGGATGC ATGAAAGCAT GAAGCTGTTC GATAGCATAT GTAACAACAA GTGGTTTACG 
GACACATCCA TCATCCTTTT CCTGAACAAG AAGGACCTCT TCGAAGAGAA GATCAAAAAG 
AGTCCCCTCA CGATATGCTA TCCAGAATAT GCAGGCTCAA ACACATATGA AGAGGCGGCT 
GCGTATATCC AGTGTCAGTT TGAAGACCTC AATAAAAGGA AGGACACAAA GGAAATTTAC 
ACCCACTTCA CTTGCGCCAC GGATACGAAG AATGTGCAGT TTGTGTTCGA TGCTGTAACG 
GACGTCATCA TAAAGAATAA CCTAAAAGAC TGTGGTCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037277.1
CDS	219..1283
Translation	MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Target ORF information:

RefSeq Version	NM_013145.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit alpha i1 (Gnai1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013145.1

ORF Insert Sequence:

ATGGGCTGCA CACTGAGCGC TGAGGACAAG GCGGCCGTGG AGCGCAGCAA GATGATCGAC 
CGCAACCTCC GGGAGGACGG AGAGAAGGCA GCGCGCGAGG TCAAGCTGCT GCTGCTGGGT 
GCTGGTGAAT CCGGGAAGAG CACAATTGTG AAGCAGATGA AAATTATCCA CGAGGCTGGC 
TACTCAGAGG AAGAGTGTAA GCAGTACAAA GCAGTGGTCT ACAGCAACAC CATCCAGTCC 
ATCATTGCCA TCATTAGAGC CATGGGGAGA TTGAAAATCG ACTTTGGAGA CGCTGCTCGT 
GCGGATGATG CTCGCCAACT CTTCGTGCTT GCTGGGGCTG CAGAGGAAGG CTTTATGACC 
GCGGAGCTCG CCGGCGTCAT AAAGAGACTG TGGAAGGACA GCGGTGTGCA AGCCTGCTTC 
AACAGATCCC GGGAGTACCA GCTGAACGAT TCGGCGGCGT ACTACCTGAA TGACTTGGAC 
AGAATAGCAC AACCAAATTA CATCCCAACC CAGCAGGATG TTCTCAGAAC TAGAGTGAAA 
ACGACGGGAA TTGTGGAAAC CCACTTTACT TTCAAAGATC TTCATTTTAA AATGTTTGAC 
GTGGGAGGCC AGAGATCAGA GCGGAAGAAG TGGATTCACT GCTTTGAAGG CGTGACTGCC 
ATCATCTTCT GTGTGGCCCT GAGTGACTAT GACCTGGTTC TTGCTGAGGA TGAAGAAATG 
AACCGGATGC ATGAAAGCAT GAAGCTGTTC GATAGCATAT GTAACAACAA GTGGTTTACG 
GACACATCCA TCATCCTTTT CCTGAACAAG AAGGACCTCT TCGAAGAGAA GATCAAAAAG 
AGTCCCCTCA CGATATGCTA TCCAGAATAT GCAGGCTCAA ACACATATGA AGAGGCGGCT 
GCGTATATCC AGTGTCAGTT TGAAGACCTC AATAAAAGGA AGGACACAAA GGAAATTTAC 
ACCCACTTCA CTTGCGCCAC GGATACGAAG AATGTGCAGT TTGTGTTCGA TGCTGTAACG 
GACGTCATCA TAAAGAATAA CCTAAAAGAC TGTGGTCTCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide-free state of heterotrimeric G proteins ?-subunit adopts a highly stable conformation.
The FEBS journal284(15)2464-2481(2017 Aug)
Andhirka SK,Vignesh R,Aradhyam GK

Effect of N-Terminal Myristoylation on the Active Conformation of G?
Biochemistry56(1)271-280(2017 Jan)
van Keulen SC,Rothlisberger U

Nanosecond Dynamics of G?i1 Bound to Nucleotides or Ric-8A, a G? Chaperone with GEF Activity.
Biophysical journal111(4)722-731(2016 Aug)
Black LA,Thomas CJ,Nix GN,Terwilliger MC,Sprang SR,Ross JBA

SAPCD2 Controls Spindle Orientation and Asymmetric Divisions by Negatively Regulating the G?i-LGN-NuMA Ternary Complex.
Developmental cell36(1)50-62(2016 Jan)
Chiu CWN,Monat C,Robitaille M,Lacomme M,Daulat AM,Macleod G,McNeill H,Cayouette M,Angers S

The Orphan G Protein-coupled Receptor GPR17 Negatively Regulates Oligodendrocyte Differentiation via G?i/o and Its Downstream Effector Molecules.
The Journal of biological chemistry291(2)705-18(2016 Jan)
Simon K,Hennen S,Merten N,Bl?ttermann S,Gillard M,Kostenis E,Gomeza J

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Gnai1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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