Mep1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mep1a
Entrez Gene ID	25684
Full Name	meprin A subunit alpha
General protein information	Preferred Names meprin A subunit alpha Names meprin A subunit alpha E-24.18 MEP-1 endopeptidase-2 endopeptidase-24.18 subunit alpha meprin 1 alpha meprin 1 subunit alpha meprin A alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q13
Summary	alpha subunit of meprin metalloproteases, which form homotetramers and heterodimers with beta subunit Mep1b [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008766838.1	XP_008765060.1	meprin A subunit alpha isoform X1
NM_013143.1	NP_037275.1	meprin A subunit alpha precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MEP1A	XP_003587735.2
Mus musculus (house mouse)	Mep1a	NP_032611.2
Xenopus tropicalis (tropical clawed frog)	mep1a	NP_001015767.1
Gallus gallus (chicken)	MEP1A	NP_001264650.1
	MEP1A	XP_001103444.1
Danio rerio (zebrafish)	mep1a.2	NP_001122199.1
Canis lupus familiaris (dog)	MEP1A	XP_538947.3
Rattus norvegicus (Norway rat)	Mep1a	NP_037275.1
Homo sapiens (human)	MEP1A	NP_005579.2
Pan troglodytes (chimpanzee)	MEP1A	XP_518520.3

Gene Ontology
Bibliography

Related articles in PubMed

Structure of homo- and hetero-oligomeric meprin metalloproteases. Dimers, tetramers, and high molecular mass multimers.
Bertenshaw GP, Norcum MT, Bond JS
The Journal of biological chemistry278(4)2522-32(2003 Jan)

Probing the active sites and mechanisms of rat metalloproteases meprin A and B.
Bertenshaw GP, Villa JP, Hengst JA, Bond JS
Biological chemistry383(7-8)1175-83(2002 Jul-Aug)

Intestinal epithelial cells secrete exosome-like vesicles.
van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M
Gastroenterology121(2)337-49(2001 Aug)

Polarised expression of human intestinal N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase (human meprin) alpha and beta subunits in Madin-Darby canine kidney cells.
Eldering JA, Gr?nberg J, Hahn D, Croes HJ, Fransen JA, Sterchi EE
European journal of biochemistry247(3)920-32(1997 Aug)

Identification of the cysteine residues implicated in the formation of alpha 2 and alpha/beta dimers of rat meprin.
Chevallier S, Ahn J, Boileau G, Crine P
The Biochemical journal317 ( Pt 3)731-8(1996 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

optimization of activity by pH and NaCl concentration and relevance to catalytic mechanism
Title: Probing the active sites and mechanisms of rat metalloproteases meprin A and B.

The following Mep1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mep1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa43198	XM_008766838.1 Latest version!	Rattus norvegicus meprin 1 subunit alpha (Mep1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa02115	NM_013143.1 Latest version!	Rattus norvegicus meprin A subunit alpha (Mep1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mep1a ( XM_008766838.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765060.1 Rattus norvegicus meprin 1 subunit alpha (Mep1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43198
Clone ID Related Accession (Same CDS sequence)	XM_008766838.1
Accession Version	XM_008766838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	meprin A subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCGCC TCAAGTCTTG TGTGGACTTC AAGCCCTATG AGGGGGAGAG CTCGTACATC 
ATCTTCCAGC AGTTTTCTGG GTGCTGGTCT ATGGTCGGTG ATCAACACGT GGGACAGAAT 
ATTTCCATTG GTGAGGGCTG CGACTATAAG GCCATCATTG AACATGAGAT CCTGCATGCT 
CTGGGATTCT TCCATGAGCA GTCAAGGACT GACCGGGATG ATTATGTGAA CATCTGGTGG 
AATGAAATTA TGACAGATTA TGAACATAAT TTCAACACGT ATGATGACAA GACCATCACA 
GACCTCAACA CACCCTATGA TTATGAGTCC TTGATGCACT ATGGGCCATT TTCATTTAAC 
AAGAATGAAA CCATCCCGAC CATCACTACC AAGATCCCTG AGTTCAACGC CATCATTGGA 
CAGCGCCTGG ATTTCAGTGC CACTGATTTA ACGAGACTGA ATCGGATGTA CAACTGCACC 
AGAACACATA CCCTGCTGGA CCACTGTGCG TTTGAGAAGA CCAACATCTG TGGAATGATC 
CAGGGGACCA GAGATGATGC TGACTGGGTT CATGAGGACA GCAGTCAGCC TGGACAAGTG 
GATCACACCT TGGTGGGACG GTGCAAAGCT GCTGGCTACT TCATGTACTT CAATACCAGC 
TCGGGGGTGA CCGGAGAGGT AGCCCTTCTG GAGTCTCGGA TCCTTTACCC CAAGAGGAAG 
CAGCAGTGTT TGCAGTTTTT TTATAAGATG ACAGGAAGCC CTGCAGACAG ACTCCTCATC 
TGGGTGAGAA GGGACGACAA CACGGGCAAC GTGTGCCAGC TGGCCAAGAT CCAGACTTTT 
CAAGGAGATT CTGATCACAA CTGGAAAATT GCTCACGTGA CACTCAACGA AGAGAAGAAA 
TTTCGCTATG TTTTCCAGGG TACCAAAGGT GACCCTGGTA ACTCAGATGG CGGTATTTAC 
CTTGATGACA TCACTCTGAC AGAAACCCCC TGCCCTACAG GGGTTTGGAC CATCCGGAAT 
ATCTCCCAGG TCCTTGAGAA CACAGTGAAA GGGGACAGGC TCGTGAGCCC TAGGTTCTAC 
AATTCCGAGG GTTATGGTTT TGGGGTGACC TTGTACCCCA ATGGTAGAAT CACCAGTAAC 
TCTGGCTACC TGGGACTCGC TTTCCACTTG TACAGTGGGG ATAACGATGT GATTCTGGAG 
TGGCCAGTGG AGAACAGACA GGCCATAATG ACCATCCTGG ACCAGGAACC TGATGCCAGG 
AACAGGATGT CCTTGAGTTT GATGTTCACT ACCTCCAAGT ACCAAACATC TTCAGCTATA 
AATGGCTCTG TCATCTGGGA CAGGCCAACC AAGGTTGGAG TCTATGATAA GGACTGTGAT 
TGTTTTAGAA GCATTGACTG GGGTTGGGGT CAGGCCATTT CCCACCAGAT GCTGATGAGA 
AGAAATTTCC TTAAAGATGA CACCCTCATC ATCTTCGTGG ACTTTAAAGA CCTTACCCAC 
TTGAGGCAGA CTGAAGTCCC CATTCCCAGC AGAAGCGTGA TACCCCGAGG ACTCCTTCTG 
CAAGGCCAGG AGCCTCTTGC CTTGGGAGAC TCCAGAATAG CCATGATGGA GGAATCCCTG 
CCAAGAAGGC TGGACCAGAG ACAGCCCAGC AGACCAAAGC GCTCAGTGGA AAATACTGGT 
CCCATGGAGG ACCACAACTG GCCACAGTAC TTCAGAGACC CATGTGACCC AAACCCTTGC 
CAGAATGAAG GCACCTGTGT GAACGTAAAG GGAATGGCCA GCTGCAGGTG CGTCTCTGGG 
CATGCCTTTT TCTACACAGG GGAGCGCTGT CAGGCTATGC ATGTGCACGG AAGCTTGCTG 
GGCCTGCTCA TTGGCTGCAT CACTGCTTTG ATCTTCTTGA CCTTCATCAC ATTCTCCAAC 
ACTTACCAAA AGCTGAGGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765060.1
CDS	197..2140
Translation	MFRLKSCVDFKPYEGESSYIIFQQFSGCWSMVGDQHVGQNISIGEGCDYKAIIEHEILHALGFFHEQSRTDRDDYVNIWWNEIMTDYEHNFNTYDDKTITDLNTPYDYESLMHYGPFSFNKNETIPTITTKIPEFNAIIGQRLDFSATDLTRLNRMYNCTRTHTLLDHCAFEKTNICGMIQGTRDDADWVHEDSSQPGQVDHTLVGRCKAAGYFMYFNTSSGVTGEVALLESRILYPKRKQQCLQFFYKMTGSPADRLLIWVRRDDNTGNVCQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFQGTKGDPGNSDGGIYLDDITLTETPCPTGVWTIRNISQVLENTVKGDRLVSPRFYNSEGYGFGVTLYPNGRITSNSGYLGLAFHLYSGDNDVILEWPVENRQAIMTILDQEPDARNRMSLSLMFTTSKYQTSSAINGSVIWDRPTKVGVYDKDCDCFRSIDWGWGQAISHQMLMRRNFLKDDTLIIFVDFKDLTHLRQTEVPIPSRSVIPRGLLLQGQEPLALGDSRIAMMEESLPRRLDQRQPSRPKRSVENTGPMEDHNWPQYFRDPCDPNPCQNEGTCVNVKGMASCRCVSGHAFFYTGERCQAMHVHGSLLGLLIGCITALIFLTFITFSNTYQKLRQ

Target ORF information:

RefSeq Version	XM_008766838.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus meprin 1 subunit alpha (Mep1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766838.1

ORF Insert Sequence:

ATGTTCCGCC TCAAGTCTTG TGTGGACTTC AAGCCCTATG AGGGGGAGAG CTCGTACATC 
ATCTTCCAGC AGTTTTCTGG GTGCTGGTCT ATGGTCGGTG ATCAACACGT GGGACAGAAT 
ATTTCCATTG GTGAGGGCTG CGACTATAAG GCCATCATTG AACATGAGAT CCTGCATGCT 
CTGGGATTCT TCCATGAGCA GTCAAGGACT GACCGGGATG ATTATGTGAA CATCTGGTGG 
AATGAAATTA TGACAGATTA TGAACATAAT TTCAACACGT ATGATGACAA GACCATCACA 
GACCTCAACA CACCCTATGA TTATGAGTCC TTGATGCACT ATGGGCCATT TTCATTTAAC 
AAGAATGAAA CCATCCCGAC CATCACTACC AAGATCCCTG AGTTCAACGC CATCATTGGA 
CAGCGCCTGG ATTTCAGTGC CACTGATTTA ACGAGACTGA ATCGGATGTA CAACTGCACC 
AGAACACATA CCCTGCTGGA CCACTGTGCG TTTGAGAAGA CCAACATCTG TGGAATGATC 
CAGGGGACCA GAGATGATGC TGACTGGGTT CATGAGGACA GCAGTCAGCC TGGACAAGTG 
GATCACACCT TGGTGGGACG GTGCAAAGCT GCTGGCTACT TCATGTACTT CAATACCAGC 
TCGGGGGTGA CCGGAGAGGT AGCCCTTCTG GAGTCTCGGA TCCTTTACCC CAAGAGGAAG 
CAGCAGTGTT TGCAGTTTTT TTATAAGATG ACAGGAAGCC CTGCAGACAG ACTCCTCATC 
TGGGTGAGAA GGGACGACAA CACGGGCAAC GTGTGCCAGC TGGCCAAGAT CCAGACTTTT 
CAAGGAGATT CTGATCACAA CTGGAAAATT GCTCACGTGA CACTCAACGA AGAGAAGAAA 
TTTCGCTATG TTTTCCAGGG TACCAAAGGT GACCCTGGTA ACTCAGATGG CGGTATTTAC 
CTTGATGACA TCACTCTGAC AGAAACCCCC TGCCCTACAG GGGTTTGGAC CATCCGGAAT 
ATCTCCCAGG TCCTTGAGAA CACAGTGAAA GGGGACAGGC TCGTGAGCCC TAGGTTCTAC 
AATTCCGAGG GTTATGGTTT TGGGGTGACC TTGTACCCCA ATGGTAGAAT CACCAGTAAC 
TCTGGCTACC TGGGACTCGC TTTCCACTTG TACAGTGGGG ATAACGATGT GATTCTGGAG 
TGGCCAGTGG AGAACAGACA GGCCATAATG ACCATCCTGG ACCAGGAACC TGATGCCAGG 
AACAGGATGT CCTTGAGTTT GATGTTCACT ACCTCCAAGT ACCAAACATC TTCAGCTATA 
AATGGCTCTG TCATCTGGGA CAGGCCAACC AAGGTTGGAG TCTATGATAA GGACTGTGAT 
TGTTTTAGAA GCATTGACTG GGGTTGGGGT CAGGCCATTT CCCACCAGAT GCTGATGAGA 
AGAAATTTCC TTAAAGATGA CACCCTCATC ATCTTCGTGG ACTTTAAAGA CCTTACCCAC 
TTGAGGCAGA CTGAAGTCCC CATTCCCAGC AGAAGCGTGA TACCCCGAGG ACTCCTTCTG 
CAAGGCCAGG AGCCTCTTGC CTTGGGAGAC TCCAGAATAG CCATGATGGA GGAATCCCTG 
CCAAGAAGGC TGGACCAGAG ACAGCCCAGC AGACCAAAGC GCTCAGTGGA AAATACTGGT 
CCCATGGAGG ACCACAACTG GCCACAGTAC TTCAGAGACC CATGTGACCC AAACCCTTGC 
CAGAATGAAG GCACCTGTGT GAACGTAAAG GGAATGGCCA GCTGCAGGTG CGTCTCTGGG 
CATGCCTTTT TCTACACAGG GGAGCGCTGT CAGGCTATGC ATGTGCACGG AAGCTTGCTG 
GGCCTGCTCA TTGGCTGCAT CACTGCTTTG ATCTTCTTGA CCTTCATCAC ATTCTCCAAC 
ACTTACCAAA AGCTGAGGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mep1a ( NM_013143.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037275.1 Rattus norvegicus meprin A subunit alpha (Mep1a), mRNA.

Price & Availability↑

CloneID	ORa02115
Clone ID Related Accession (Same CDS sequence)	NM_013143.1
Accession Version	NM_013143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	meprin A subunit alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S43408.1. On Jun 25, 2006 this sequence version replaced XM_001068729.1. ##Evidence-Data-START## Transcript exon combination :: BC081834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642745 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTGGA CACTACCTGT GTGTCTTCTC TCACTGAGTT TTTCTGCCCA CATTGCAGCT 
GTGTCCATTC AGCATCTTTC GACTGGACAT GACCACGATG ATGTAGACGT TGGTGAACAA 
CAGAAGGACA TTTCAGAAAT CAACTCAGCT GCAGGCCTGA ACCTTTTTCA AGGGGACATC 
CTTCTGCCCA GAACCAGAAA TGCCCTGAGA GATCCGTCAA GCAGATGGAA GCCCCCCATT 
CCATACATTC TGGCTGACAA TCTGGATCTG AACGCCAAAG GAGCCATCCT CAATGCCTTT 
GAGATGTTCC GCCTCAAGTC TTGTGTGGAC TTCAAGCCCT ATGAGGGGGA GAGCTCGTAC 
ATCATCTTCC AGCAGTTTTC TGGGTGCTGG TCTATGGTCG GTGATCAACA CGTGGGACAG 
AATATTTCCA TTGGTGAGGG CTGCGACTAT AAGGCCATCA TTGAACATGA GATCCTGCAT 
GCTCTGGGAT TCTTCCATGA GCAGTCAAGG ACTGACCGGG ATGATTATGT GAACATCTGG 
TGGAATGAAA TTATGACAGA TTATGAACAT AATTTCAACA CGTATGATGA CAAGACCATC 
ACAGACCTCA ACACACCCTA TGATTATGAG TCCTTGATGC ACTATGGGCC ATTTTCATTT 
AACAAGAATG AAACCATCCC GACCATCACT ACCAAGATCC CTGAGTTCAA CGCCATCATT 
GGACAGCGCC TGGATTTCAG TGCCACTGAT TTAACGAGAC TGAATCGGAT GTACAACTGC 
ACCAGAACAC ATACCCTGCT GGACCACTGT GCGTTTGAGA AGACCAACAT CTGTGGAATG 
ATCCAGGGGA CCAGAGATGA TGCTGACTGG GTTCATGAGG ACAGCAGTCA GCCTGGACAA 
GTGGATCACA CCTTGGTGGG ACGGTGCAAA GCTGCTGGCT ACTTCATGTA CTTCAATACC 
AGCTCGGGGG TGACCGGAGA GGTAGCCCTT CTGGAGTCTC GGATCCTTTA CCCCAAGAGG 
AAGCAGCAGT GTTTGCAGTT TTTTTATAAG ATGACAGGAA GCCCTTCAGA CAGACTCCTC 
ATCTGGGTGA GAAGGGACGA CAACACGGGC AACGTGCGCC AGCTGGCCAA GATCCAGACT 
TTTCAAGGAG ATTCTGATCA CAACTGGAAA ATTGCTCACG TGACACTCAA CGAAGAGAAG 
AAATTTCGCT ATGTTTTCCA GGGTACCAAA GGTGACCCTG GTAACTCAGA TGGCGGTATT 
TACCTTGATG ACATCACTCT GACAGAAACC CCCTGCCCTA CAGGGGTTTG GACCATCCGG 
AATATCTCCC AGGTCCTTGA GAACACAGTG AAAGGGGACA GGCTCGTGAG CCCTAGGTTC 
TACAATTCCG AGGGTTATGG TTTTGGGGTG ACCTTGTACC CCAATGGTAG AATCACCAGT 
AACTCTGGCT ACCTGGGACT CGCTTTCCAC TTGTACAGTG GGGATAACGA TGTGATTCTG 
GAGTGGCCAG TGGAGAACGA GCAGGCCATA ATGACCATCC TGGACCAGGA ACCTGATGCC 
AGGAACAGGA TGTCCTTGAG TTTGATGTTC ACTACCTCCA AGTACCAAAC ATCTTCAGCT 
ATAAATGGCT CTGTCATCTG GGACAGGCCA ACCAAGGTTG GAGTCTATGA TAAGGACTGT 
GATTGTTTTA GAAGCATTGA CTGGGGTTGG GGTCAGGCCA TTTCCCACCA GATGCTGATG 
AGAAGAAATT TCCTTAAAGA TGACACCCTC ATCATCTTCG TGGACTTTAA AGACCTTACC 
CACTTGAGGC AGACTGAAGT CCCCATTTCC AGCAGAAGCG TGATACCCCG AGGACTCCTT 
CTGCAAGGCC AGGAGCCTCT TGCCTTGGGA GACTCCAGAA TAGCCATGAT GGAGGAATCC 
CTGCCAAGAA GGCTGGACCA GAGACAGCCC AGCAGACCAA AGCGCTCAGT GGAAAATACT 
GGTCCCATGG AGGACCACAA CTGGCCACAG TACTTCAGAG ACCCATGTGA CCCAAACCCT 
TGCCAGAATG AAGGCACCTG TGTGAACGTA AAGGGAATGG CCAGCTGCAG GTGCGTCTCT 
GGGCATGCCT TTTTCTACAC AGGGGAGCGC TGTCAGGCTA TGCATGTGCA CGGAAGCTTG 
CTGGGCCTGC TCATTGGCTG CATCACTGCT TTGATCTTCT TGACCTTCAT CACATTCTCC 
AACACTTACC AAAAGCTGAG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037275.1
CDS	33..2279
Translation	MLWTLPVCLLSLSFSAHIAAVSIQHLSTGHDHDDVDVGEQQKDISEINSAAGLNLFQGDILLPRTRNALRDPSSRWKPPIPYILADNLDLNAKGAILNAFEMFRLKSCVDFKPYEGESSYIIFQQFSGCWSMVGDQHVGQNISIGEGCDYKAIIEHEILHALGFFHEQSRTDRDDYVNIWWNEIMTDYEHNFNTYDDKTITDLNTPYDYESLMHYGPFSFNKNETIPTITTKIPEFNAIIGQRLDFSATDLTRLNRMYNCTRTHTLLDHCAFEKTNICGMIQGTRDDADWVHEDSSQPGQVDHTLVGRCKAAGYFMYFNTSSGVTGEVALLESRILYPKRKQQCLQFFYKMTGSPSDRLLIWVRRDDNTGNVRQLAKIQTFQGDSDHNWKIAHVTLNEEKKFRYVFQGTKGDPGNSDGGIYLDDITLTETPCPTGVWTIRNISQVLENTVKGDRLVSPRFYNSEGYGFGVTLYPNGRITSNSGYLGLAFHLYSGDNDVILEWPVENEQAIMTILDQEPDARNRMSLSLMFTTSKYQTSSAINGSVIWDRPTKVGVYDKDCDCFRSIDWGWGQAISHQMLMRRNFLKDDTLIIFVDFKDLTHLRQTEVPISSRSVIPRGLLLQGQEPLALGDSRIAMMEESLPRRLDQRQPSRPKRSVENTGPMEDHNWPQYFRDPCDPNPCQNEGTCVNVKGMASCRCVSGHAFFYTGERCQAMHVHGSLLGLLIGCITALIFLTFITFSNTYQKLRQ

Target ORF information:

RefSeq Version	NM_013143.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus meprin A subunit alpha (Mep1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013143.1

ORF Insert Sequence:

ATGCTGTGGA CACTACCTGT GTGTCTTCTC TCACTGAGTT TTTCTGCCCA CATTGCAGCT 
GTGTCCATTC AGCATCTTTC GACTGGACAT GACCACGATG ATGTAGACGT TGGTGAACAA 
CAGAAGGACA TTTCAGAAAT CAACTCAGCT GCAGGCCTGA ACCTTTTTCA AGGGGACATC 
CTTCTGCCCA GAACCAGAAA TGCCCTGAGA GATCCGTCAA GCAGATGGAA GCCCCCCATT 
CCATACATTC TGGCTGACAA TCTGGATCTG AACGCCAAAG GAGCCATCCT CAATGCCTTT 
GAGATGTTCC GCCTCAAGTC TTGTGTGGAC TTCAAGCCCT ATGAGGGGGA GAGCTCGTAC 
ATCATCTTCC AGCAGTTTTC TGGGTGCTGG TCTATGGTCG GTGATCAACA CGTGGGACAG 
AATATTTCCA TTGGTGAGGG CTGCGACTAT AAGGCCATCA TTGAACATGA GATCCTGCAT 
GCTCTGGGAT TCTTCCATGA GCAGTCAAGG ACTGACCGGG ATGATTATGT GAACATCTGG 
TGGAATGAAA TTATGACAGA TTATGAACAT AATTTCAACA CGTATGATGA CAAGACCATC 
ACAGACCTCA ACACACCCTA TGATTATGAG TCCTTGATGC ACTATGGGCC ATTTTCATTT 
AACAAGAATG AAACCATCCC GACCATCACT ACCAAGATCC CTGAGTTCAA CGCCATCATT 
GGACAGCGCC TGGATTTCAG TGCCACTGAT TTAACGAGAC TGAATCGGAT GTACAACTGC 
ACCAGAACAC ATACCCTGCT GGACCACTGT GCGTTTGAGA AGACCAACAT CTGTGGAATG 
ATCCAGGGGA CCAGAGATGA TGCTGACTGG GTTCATGAGG ACAGCAGTCA GCCTGGACAA 
GTGGATCACA CCTTGGTGGG ACGGTGCAAA GCTGCTGGCT ACTTCATGTA CTTCAATACC 
AGCTCGGGGG TGACCGGAGA GGTAGCCCTT CTGGAGTCTC GGATCCTTTA CCCCAAGAGG 
AAGCAGCAGT GTTTGCAGTT TTTTTATAAG ATGACAGGAA GCCCTTCAGA CAGACTCCTC 
ATCTGGGTGA GAAGGGACGA CAACACGGGC AACGTGCGCC AGCTGGCCAA GATCCAGACT 
TTTCAAGGAG ATTCTGATCA CAACTGGAAA ATTGCTCACG TGACACTCAA CGAAGAGAAG 
AAATTTCGCT ATGTTTTCCA GGGTACCAAA GGTGACCCTG GTAACTCAGA TGGCGGTATT 
TACCTTGATG ACATCACTCT GACAGAAACC CCCTGCCCTA CAGGGGTTTG GACCATCCGG 
AATATCTCCC AGGTCCTTGA GAACACAGTG AAAGGGGACA GGCTCGTGAG CCCTAGGTTC 
TACAATTCCG AGGGTTATGG TTTTGGGGTG ACCTTGTACC CCAATGGTAG AATCACCAGT 
AACTCTGGCT ACCTGGGACT CGCTTTCCAC TTGTACAGTG GGGATAACGA TGTGATTCTG 
GAGTGGCCAG TGGAGAACGA GCAGGCCATA ATGACCATCC TGGACCAGGA ACCTGATGCC 
AGGAACAGGA TGTCCTTGAG TTTGATGTTC ACTACCTCCA AGTACCAAAC ATCTTCAGCT 
ATAAATGGCT CTGTCATCTG GGACAGGCCA ACCAAGGTTG GAGTCTATGA TAAGGACTGT 
GATTGTTTTA GAAGCATTGA CTGGGGTTGG GGTCAGGCCA TTTCCCACCA GATGCTGATG 
AGAAGAAATT TCCTTAAAGA TGACACCCTC ATCATCTTCG TGGACTTTAA AGACCTTACC 
CACTTGAGGC AGACTGAAGT CCCCATTTCC AGCAGAAGCG TGATACCCCG AGGACTCCTT 
CTGCAAGGCC AGGAGCCTCT TGCCTTGGGA GACTCCAGAA TAGCCATGAT GGAGGAATCC 
CTGCCAAGAA GGCTGGACCA GAGACAGCCC AGCAGACCAA AGCGCTCAGT GGAAAATACT 
GGTCCCATGG AGGACCACAA CTGGCCACAG TACTTCAGAG ACCCATGTGA CCCAAACCCT 
TGCCAGAATG AAGGCACCTG TGTGAACGTA AAGGGAATGG CCAGCTGCAG GTGCGTCTCT 
GGGCATGCCT TTTTCTACAC AGGGGAGCGC TGTCAGGCTA TGCATGTGCA CGGAAGCTTG 
CTGGGCCTGC TCATTGGCTG CATCACTGCT TTGATCTTCT TGACCTTCAT CACATTCTCC 
AACACTTACC AAAAGCTGAG GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of homo- and hetero-oligomeric meprin metalloproteases. Dimers, tetramers, and high molecular mass multimers.
The Journal of biological chemistry278(4)2522-32(2003 Jan)
Bertenshaw GP,Norcum MT,Bond JS

Probing the active sites and mechanisms of rat metalloproteases meprin A and B.
Biological chemistry383(7-8)1175-83(2002 Jul-Aug)
Bertenshaw GP,Villa JP,Hengst JA,Bond JS

Intestinal epithelial cells secrete exosome-like vesicles.
Gastroenterology121(2)337-49(2001 Aug)
van Niel G,Raposo G,Candalh C,Boussac M,Hershberg R,Cerf-Bensussan N,Heyman M

Polarised expression of human intestinal N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase (human meprin) alpha and beta subunits in Madin-Darby canine kidney cells.
European journal of biochemistry247(3)920-32(1997 Aug)
Eldering JA,Gr?nberg J,Hahn D,Croes HJ,Fransen JA,Sterchi EE

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