Smad1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smad1
Entrez Gene ID	25671
Full Name	SMAD family member 1
Synonyms	Madh1
General protein information	Preferred Names SMAD family member 1 Names mothers against decapentaplegic homolog 1 MAD (mothers against decapentaplegic, Drosophila) homolog 1 MAD homolog 1 MAD homolog1 (mothers against decapentaplegic, Drosophila) SMAD 1 SMAD, mothers against DPP homolog 1 mothers against DPP homolog 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	a transcriptional activator; activated by bone morphogenetic protein 4 (BMP4) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255395.3	XP_006255457.1	mothers against decapentaplegic homolog 1 isoform X1
XM_006255397.1	XP_006255459.1	mothers against decapentaplegic homolog 1 isoform X2
XM_006255396.3	XP_006255458.1	mothers against decapentaplegic homolog 1 isoform X1
XM_006255394.1	XP_006255456.1	mothers against decapentaplegic homolog 1 isoform X1
NM_013130.2	NP_037262.2	mothers against decapentaplegic homolog 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMAD1	XP_001148403.1
Xenopus tropicalis (tropical clawed frog)	smad1	NP_001007481.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008551	XP_314661.3
Homo sapiens (human)	SMAD1	NP_001003688.1
Bos taurus (cattle)	SMAD1	NP_001069691.2
Gallus gallus (chicken)	SMAD1	NP_001188384.1
Canis lupus familiaris (dog)	SMAD1	XP_005629368.1
Rattus norvegicus (Norway rat)	Smad1	NP_037262.2
	SMAD1	XP_001096679.1
Mus musculus (house mouse)	Smad1	NP_032565.2
Danio rerio (zebrafish)	smad1	NP_571431.1
Drosophila melanogaster (fruit fly)	Mad	NP_477017.1

Gene Ontology
Bibliography

Related articles in PubMed

miR?203?3p participates in the suppression of diabetes?associated osteogenesis in the jaw bone through targeting Smad1.
Tang Y, Zheng L, Zhou J, Chen Y, Yang L, Deng F, Hu Y
International journal of molecular medicine41(3)1595-1607(2018 Mar)

Actin cytoskeleton mediates BMP2-Smad signaling via calponin 1 in preosteoblast under simulated microgravity.
Xu H, Wu F, Zhang H, Yang C, Li K, Wang H, Yang H, Liu Y, Ding B, Tan Y, Yuan M, Li Y, Dai Z
Biochimie138184-193(2017 Jul)

Cx43- and Smad-Mediated TGF-?/ BMP Signaling Pathway Promotes Cartilage Differentiation of Bone Marrow Mesenchymal Stem Cells and Inhibits Osteoblast Differentiation.
Zhang YD, Zhao SC, Zhu ZS, Wang YF, Liu JX, Zhang ZC, Xue F
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology42(4)1277-1293(2017)

Alteration of TGF-?-ALK-Smad signaling in hyperoxia-induced bronchopulmonary dysplasia model of newborn rats.
Jin M, Lee J, Lee KY, Jin Z, Pak JH, Kim HS
Experimental lung research42(7)354-364(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Actin cytoskeleton depolymerization inhibites BMP2 signaling via blocking of Smad by dephosphorylated CNN1 in osteoblast cells under simulated microgravity.
Title: Actin cytoskeleton mediates BMP2-Smad signaling via calponin 1 in preosteoblast under simulated microgravity.

Cx43 and Smad1 promote BMP2-induced cartilage differentiation of bone marrow mesenchymal stem cells and inhibit osteoblast differentiation, which provide a new strategy for cartilage tissue engineering using exogenous Cx43 and Smad1.
Title: Cx43- and Smad-Mediated TGF-?/ BMP Signaling Pathway Promotes Cartilage Differentiation of Bone Marrow Mesenchymal Stem Cells and Inhibits Osteoblast Differentiation.

exposure to hyperoxia altered the balance between TGF-beta-ALK1-Smad1/5 and TGF-beta-ALK5-Smad2/3 pathways in pulmonary endothelial cells, which may ultimately lead to the development of BPD.
Title: Alteration of TGF-?-ALK-Smad signaling in hyperoxia-induced bronchopulmonary dysplasia model of newborn rats.

Cpd 861 markedly upregulated the gene expression of BMP7 and Smad5, as well as the protein expression of BMP7 and Smad1/5. In addition, p-Smad1/5/8 protein expression was markedly increased by Cpd 861 in the liver fibrosis model.
Title: Attenuation of liver fibrosis by herbal compound?861 via upregulation of BMP-7/Smad signaling in the bile duct ligation model rat.

Taken together, these results indicated that FSTL1 protects the SNP-induced injury in cardiac H9c2 cells through, at least in part, the activation of Akt and inhibition of Smad1/5/9 signaling.
Title: Follistatin-like 1 protects cardiomyoblasts from injury induced by sodium nitroprusside through modulating Akt and Smad1/5/9 signaling.

The following Smad1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smad1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02086	NM_013130.2 Latest version!	Rattus norvegicus SMAD family member 1 (Smad1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$272.30 $389.00
ORa38790	XM_006255394.1 Latest version!	Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa38790	XM_006255396.3 Latest version!	Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa38790	XM_006255395.3 Latest version!	Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa38791	XM_006255397.1 Latest version!	Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smad1 ( NM_013130.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037262.2 Rattus norvegicus SMAD family member 1 (Smad1), mRNA.

Price & Availability↑

CloneID	ORa02086
Clone ID Related Accession (Same CDS sequence)	NM_013130.2
Accession Version	NM_013130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Rattus norvegicus(Norway rat)
Product	mothers against decapentaplegic homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061757.1. On Dec 20, 2003 this sequence version replaced NM_013130.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061757.1, U66478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCCAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGATGTTC AAGCAGTTGC TTACGAGGAA CCAAAACACT GGTGCTCTAT TGTGTACTAT 
GAGCTCAACA ACCGTGTGGG CGAAGCGTTC CATGCCTCCT CCACCAGCGT GTTGGTGGAT 
GGTTTCACCG ATCCTTCCAA CAATAAGAAC CGATTCTGCC TTGGGCTGCT CTCCAATGTT 
AACCGGAACT CCACTATTGA AAATACCAGG CGACACATCG GGAAAGGAGT CCACCTTTAT 
TACGTCGGAG GAGAGGTGTA TGCGGAGTGC CTCAGTGACA GCAGCATCTT CGTGCAGAGT 
CGGAACTGCA ACTACCACCA TGGCTTTCAT CCCACCACGG TCTGCAAGAT CCCCAGCGGG 
TGCAGCTTGA AAATCTTCAA CAACCAAGAG TTCGCTCAGC TACTGGCGCA GTCTGTGAAC 
CATGGGTTCG AGACAGTGTA TGAACTCACC AAAATGTGCA CTATTCGAAT GAGCTTCGTG 
AAGGGCTGGG GAGCGGAGTA CCACCGGCAG GATGTCACTA GCACCCCCTG CTGGATTGAG 
ATACACCCGC ATGGCCCTCT CCAGTGGCTG GATAAAGTCC TTACCCAGAT GGGTTCGCCC 
CACAACCCTA TTTCGTCGGT GTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037262.2
CDS	335..1741
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPHSHPFPQSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMTNMTAPTLPAEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHPHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	NM_013130.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD family member 1 (Smad1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013130.2

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCCAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGATGTTC AAGCAGTTGC TTACGAGGAA CCAAAACACT GGTGCTCTAT TGTGTACTAT 
GAGCTCAACA ACCGTGTGGG CGAAGCGTTC CATGCCTCCT CCACCAGCGT GTTGGTGGAT 
GGTTTCACCG ATCCTTCCAA CAATAAGAAC CGATTCTGCC TTGGGCTGCT CTCCAATGTT 
AACCGGAACT CCACTATTGA AAATACCAGG CGACACATCG GGAAAGGAGT CCACCTTTAT 
TACGTCGGAG GAGAGGTGTA TGCGGAGTGC CTCAGTGACA GCAGCATCTT CGTGCAGAGT 
CGGAACTGCA ACTACCACCA TGGCTTTCAT CCCACCACGG TCTGCAAGAT CCCCAGCGGG 
TGCAGCTTGA AAATCTTCAA CAACCAAGAG TTCGCTCAGC TACTGGCGCA GTCTGTGAAC 
CATGGGTTCG AGACAGTGTA TGAACTCACC AAAATGTGCA CTATTCGAAT GAGCTTCGTG 
AAGGGCTGGG GAGCGGAGTA CCACCGGCAG GATGTCACTA GCACCCCCTG CTGGATTGAG 
ATACACCCGC ATGGCCCTCT CCAGTGGCTG GATAAAGTCC TTACCCAGAT GGGTTCGCCC 
CACAACCCTA TTTCGTCGGT GTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smad1 ( XM_006255395.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255457.1 Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38790
Clone ID Related Accession (Same CDS sequence)	XM_006255395.3 , XM_006255396.3 , XM_006255394.1
Accession Version	XM_006255395.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mothers against decapentaplegic homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCGAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGGTGAAG TCAGCTGCCG CTCTCCCTGT GAGAGTTTGT TGTCTGCAGA TGTTCAAGCA 
GTTGCTTACG AGGAACCAAA ACACTGGTGC TCTATTGTGT ACTATGAGCT CAACAACCGT 
GTGGGCGAAG CGTTCCATGC CTCCTCCACC AGCGTGTTGG TGGATGGTTT CACCGATCCT 
TCCAACAATA AGAACCGATT CTGCCTTGGG CTGCTCTCCA ATGTTAACCG GAACTCCACT 
ATTGAAAATA CCAGGCGACA CATCGGGAAA GGAGTCCACC TTTATTACGT CGGAGGAGAG 
GTGTATGCGG AGTGCCTCAG TGACAGCAGC ATCTTCGTGC AGAGTCGGAA CTGCAACTAC 
CACCATGGCT TTCATCCCAC CACGGTCTGC AAGATCCCCA GCGGGTGCAG CTTGAAAATC 
TTCAACAACC AAGAGTTCGC TCAGCTACTG GCGCAGTCTG TGAACCATGG GTTCGAGACA 
GTGTATGAAC TCACCAAAAT GTGCACTATT CGAATGAGCT TCGTGAAGGG CTGGGGAGCA 
GAGTACCACC GGCAGGATGT CACTAGCACC CCCTGCTGGA TTGAGATACA CCTGCATGGC 
CCTCTCCAGT GGCTGGATAA AGTCCTTACC CAGATGGGTT CGCCCCACAA CCCTATTTCG 
TCGGTGTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255457.1
CDS	6430..7881
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPHSHPFPQSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMTNMTAPTLPAEINRGGEVSCRSPCESLLSADVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	XM_006255395.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255395.3

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCGAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGGTGAAG TCAGCTGCCG CTCTCCCTGT GAGAGTTTGT TGTCTGCAGA TGTTCAAGCA 
GTTGCTTACG AGGAACCAAA ACACTGGTGC TCTATTGTGT ACTATGAGCT CAACAACCGT 
GTGGGCGAAG CGTTCCATGC CTCCTCCACC AGCGTGTTGG TGGATGGTTT CACCGATCCT 
TCCAACAATA AGAACCGATT CTGCCTTGGG CTGCTCTCCA ATGTTAACCG GAACTCCACT 
ATTGAAAATA CCAGGCGACA CATCGGGAAA GGAGTCCACC TTTATTACGT CGGAGGAGAG 
GTGTATGCGG AGTGCCTCAG TGACAGCAGC ATCTTCGTGC AGAGTCGGAA CTGCAACTAC 
CACCATGGCT TTCATCCCAC CACGGTCTGC AAGATCCCCA GCGGGTGCAG CTTGAAAATC 
TTCAACAACC AAGAGTTCGC TCAGCTACTG GCGCAGTCTG TGAACCATGG GTTCGAGACA 
GTGTATGAAC TCACCAAAAT GTGCACTATT CGAATGAGCT TCGTGAAGGG CTGGGGAGCA 
GAGTACCACC GGCAGGATGT CACTAGCACC CCCTGCTGGA TTGAGATACA CCTGCATGGC 
CCTCTCCAGT GGCTGGATAA AGTCCTTACC CAGATGGGTT CGCCCCACAA CCCTATTTCG 
TCGGTGTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smad1 ( XM_006255396.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255458.1 Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa38790
Clone ID Related Accession (Same CDS sequence)	XM_006255395.3 , XM_006255396.3 , XM_006255394.1
Accession Version	XM_006255396.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mothers against decapentaplegic homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCGAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGGTGAAG TCAGCTGCCG CTCTCCCTGT GAGAGTTTGT TGTCTGCAGA TGTTCAAGCA 
GTTGCTTACG AGGAACCAAA ACACTGGTGC TCTATTGTGT ACTATGAGCT CAACAACCGT 
GTGGGCGAAG CGTTCCATGC CTCCTCCACC AGCGTGTTGG TGGATGGTTT CACCGATCCT 
TCCAACAATA AGAACCGATT CTGCCTTGGG CTGCTCTCCA ATGTTAACCG GAACTCCACT 
ATTGAAAATA CCAGGCGACA CATCGGGAAA GGAGTCCACC TTTATTACGT CGGAGGAGAG 
GTGTATGCGG AGTGCCTCAG TGACAGCAGC ATCTTCGTGC AGAGTCGGAA CTGCAACTAC 
CACCATGGCT TTCATCCCAC CACGGTCTGC AAGATCCCCA GCGGGTGCAG CTTGAAAATC 
TTCAACAACC AAGAGTTCGC TCAGCTACTG GCGCAGTCTG TGAACCATGG GTTCGAGACA 
GTGTATGAAC TCACCAAAAT GTGCACTATT CGAATGAGCT TCGTGAAGGG CTGGGGAGCA 
GAGTACCACC GGCAGGATGT CACTAGCACC CCCTGCTGGA TTGAGATACA CCTGCATGGC 
CCTCTCCAGT GGCTGGATAA AGTCCTTACC CAGATGGGTT CGCCCCACAA CCCTATTTCG 
TCGGTGTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255458.1
CDS	298..1749
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPHSHPFPQSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMTNMTAPTLPAEINRGGEVSCRSPCESLLSADVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	XM_006255396.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255396.3

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCGAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGGTGAAG TCAGCTGCCG CTCTCCCTGT GAGAGTTTGT TGTCTGCAGA TGTTCAAGCA 
GTTGCTTACG AGGAACCAAA ACACTGGTGC TCTATTGTGT ACTATGAGCT CAACAACCGT 
GTGGGCGAAG CGTTCCATGC CTCCTCCACC AGCGTGTTGG TGGATGGTTT CACCGATCCT 
TCCAACAATA AGAACCGATT CTGCCTTGGG CTGCTCTCCA ATGTTAACCG GAACTCCACT 
ATTGAAAATA CCAGGCGACA CATCGGGAAA GGAGTCCACC TTTATTACGT CGGAGGAGAG 
GTGTATGCGG AGTGCCTCAG TGACAGCAGC ATCTTCGTGC AGAGTCGGAA CTGCAACTAC 
CACCATGGCT TTCATCCCAC CACGGTCTGC AAGATCCCCA GCGGGTGCAG CTTGAAAATC 
TTCAACAACC AAGAGTTCGC TCAGCTACTG GCGCAGTCTG TGAACCATGG GTTCGAGACA 
GTGTATGAAC TCACCAAAAT GTGCACTATT CGAATGAGCT TCGTGAAGGG CTGGGGAGCA 
GAGTACCACC GGCAGGATGT CACTAGCACC CCCTGCTGGA TTGAGATACA CCTGCATGGC 
CCTCTCCAGT GGCTGGATAA AGTCCTTACC CAGATGGGTT CGCCCCACAA CCCTATTTCG 
TCGGTGTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smad1 ( XM_006255394.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255456.1 Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X1, mRNA.

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CloneID	ORa38790
Clone ID Related Accession (Same CDS sequence)	XM_006255395.3 , XM_006255396.3 , XM_006255394.1
Accession Version	XM_006255394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mothers against decapentaplegic homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCGAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGGTGAAG TCAGCTGCCG CTCTCCCTGT GAGAGTTTGT TGTCTGCAGA TGTTCAAGCA 
GTTGCTTACG AGGAACCAAA ACACTGGTGC TCTATTGTGT ACTATGAGCT CAACAACCGT 
GTGGGCGAAG CGTTCCATGC CTCCTCCACC AGCGTGTTGG TGGATGGTTT CACCGATCCT 
TCCAACAATA AGAACCGATT CTGCCTTGGG CTGCTCTCCA ATGTTAACCG GAACTCCACT 
ATTGAAAATA CCAGGCGACA CATCGGGAAA GGAGTCCACC TTTATTACGT CGGAGGAGAG 
GTGTATGCGG AGTGCCTCAG TGACAGCAGC ATCTTCGTGC AGAGTCGGAA CTGCAACTAC 
CACCATGGCT TTCATCCCAC CACGGTCTGC AAGATCCCCA GCGGGTGCAG CTTGAAAATC 
TTCAACAACC AAGAGTTCGC TCAGCTACTG GCGCAGTCTG TGAACCATGG GTTCGAGACA 
GTGTATGAAC TCACCAAAAT GTGCACTATT CGAATGAGCT TCGTGAAGGG CTGGGGAGCA 
GAGTACCACC GGCAGGATGT CACTAGCACC CCCTGCTGGA TTGAGATACA CCTGCATGGC 
CCTCTCCAGT GGCTGGATAA AGTCCTTACC CAGATGGGTT CGCCCCACAA CCCTATTTCG 
TCGGTGTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255456.1
CDS	436..1887
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPHSHPFPQSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMTNMTAPTLPAEINRGGEVSCRSPCESLLSADVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	XM_006255394.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255394.1

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCGAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGGTGAAG TCAGCTGCCG CTCTCCCTGT GAGAGTTTGT TGTCTGCAGA TGTTCAAGCA 
GTTGCTTACG AGGAACCAAA ACACTGGTGC TCTATTGTGT ACTATGAGCT CAACAACCGT 
GTGGGCGAAG CGTTCCATGC CTCCTCCACC AGCGTGTTGG TGGATGGTTT CACCGATCCT 
TCCAACAATA AGAACCGATT CTGCCTTGGG CTGCTCTCCA ATGTTAACCG GAACTCCACT 
ATTGAAAATA CCAGGCGACA CATCGGGAAA GGAGTCCACC TTTATTACGT CGGAGGAGAG 
GTGTATGCGG AGTGCCTCAG TGACAGCAGC ATCTTCGTGC AGAGTCGGAA CTGCAACTAC 
CACCATGGCT TTCATCCCAC CACGGTCTGC AAGATCCCCA GCGGGTGCAG CTTGAAAATC 
TTCAACAACC AAGAGTTCGC TCAGCTACTG GCGCAGTCTG TGAACCATGG GTTCGAGACA 
GTGTATGAAC TCACCAAAAT GTGCACTATT CGAATGAGCT TCGTGAAGGG CTGGGGAGCA 
GAGTACCACC GGCAGGATGT CACTAGCACC CCCTGCTGGA TTGAGATACA CCTGCATGGC 
CCTCTCCAGT GGCTGGATAA AGTCCTTACC CAGATGGGTT CGCCCCACAA CCCTATTTCG 
TCGGTGTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smad1 ( XM_006255397.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255459.1 Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa38791
Clone ID Related Accession (Same CDS sequence)	XM_006255397.1
Accession Version	XM_006255397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mothers against decapentaplegic homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCGAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGGTGAAG TCAGCTGCCG CTCTCCCTAT GTTCAAGCAG TTGCTTACGA GGAACCAAAA 
CACTGGTGCT CTATTGTGTA CTATGAGCTC AACAACCGTG TGGGCGAAGC GTTCCATGCC 
TCCTCCACCA GCGTGTTGGT GGATGGTTTC ACCGATCCTT CCAACAATAA GAACCGATTC 
TGCCTTGGGC TGCTCTCCAA TGTTAACCGG AACTCCACTA TTGAAAATAC CAGGCGACAC 
ATCGGGAAAG GAGTCCACCT TTATTACGTC GGAGGAGAGG TGTATGCGGA GTGCCTCAGT 
GACAGCAGCA TCTTCGTGCA GAGTCGGAAC TGCAACTACC ACCATGGCTT TCATCCCACC 
ACGGTCTGCA AGATCCCCAG CGGGTGCAGC TTGAAAATCT TCAACAACCA AGAGTTCGCT 
CAGCTACTGG CGCAGTCTGT GAACCATGGG TTCGAGACAG TGTATGAACT CACCAAAATG 
TGCACTATTC GAATGAGCTT CGTGAAGGGC TGGGGAGCAG AGTACCACCG GCAGGATGTC 
ACTAGCACCC CCTGCTGGAT TGAGATACAC CTGCATGGCC CTCTCCAGTG GCTGGATAAA 
GTCCTTACCC AGATGGGTTC GCCCCACAAC CCTATTTCGT CGGTGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255459.1
CDS	436..1866
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPHSHPFPQSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMAQDGSQPMDTNMTNMTAPTLPAEINRGGEVSCRSPYVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	XM_006255397.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMAD family member 1 (Smad1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255397.1

ORF Insert Sequence:

ATGAATGTGA CCAGCTTGTT TTCATTCACA AGCCCAGCTG TGAAGAGACT CCTTGGGTGG 
AAACAGGGCG ACGAAGAAGA GAAATGGGCG GAGAAGGCTG TGGACGCTTT GGTGAAGAAA 
CTGAAGAAGA AGAAAGGGGC CATGGAAGAG CTGGAGAAGG CCCTGAGCTG CCCCGGGCAG 
CCGAGTAACT GTGTCACCAT TCCTCGCTCC CTGGATGGCA GGTTGCAGGT GTCCCACCGG 
AAGGGACTGC CTCATGTCAT TTATTGCCGC GTGTGGCGCT GGCCCGACCT CCAGAGCCAT 
CATGAACTAA AGCCTCTGGA ATGCTGCGAG TTCCCATTTG GTTCCAAGCA GAAGGAAGTC 
TGCATCAATC CCTACCACTA TAAGCGAGTG GAGAGCCCCG TTCTCCCACC GGTGCTGGTT 
CCGAGGCACA GCGAGTACAA CCCTCAGCAC AGCCTTCTGG CTCAGTTCCG AAACCTGGGA 
CAAAATGAGC CCCACATGCC ACTGAATGCC ACGTTCCCCG ACTCCTTCCA GCAGCCACAC 
AGCCACCCGT TTCCCCAGTC CCCCAACAGC AGCTACCCCA ACTCTCCTGG CAGCAGCAGC 
AGCACCTACC CTCACTCCCC GACCAGCTCA GACCCGGGCA GCCCTTTTCA GATGCCAGCC 
GACACACCCC CGCCTGCTTA CCTGCCTCCT GAAGACCCCA TGGCCCAGGA TGGCTCTCAG 
CCTATGGACA CGAACATGAC GAACATGACG GCACCGACAC TGCCCGCGGA GATCAATAGA 
GGAGGTGAAG TCAGCTGCCG CTCTCCCTAT GTTCAAGCAG TTGCTTACGA GGAACCAAAA 
CACTGGTGCT CTATTGTGTA CTATGAGCTC AACAACCGTG TGGGCGAAGC GTTCCATGCC 
TCCTCCACCA GCGTGTTGGT GGATGGTTTC ACCGATCCTT CCAACAATAA GAACCGATTC 
TGCCTTGGGC TGCTCTCCAA TGTTAACCGG AACTCCACTA TTGAAAATAC CAGGCGACAC 
ATCGGGAAAG GAGTCCACCT TTATTACGTC GGAGGAGAGG TGTATGCGGA GTGCCTCAGT 
GACAGCAGCA TCTTCGTGCA GAGTCGGAAC TGCAACTACC ACCATGGCTT TCATCCCACC 
ACGGTCTGCA AGATCCCCAG CGGGTGCAGC TTGAAAATCT TCAACAACCA AGAGTTCGCT 
CAGCTACTGG CGCAGTCTGT GAACCATGGG TTCGAGACAG TGTATGAACT CACCAAAATG 
TGCACTATTC GAATGAGCTT CGTGAAGGGC TGGGGAGCAG AGTACCACCG GCAGGATGTC 
ACTAGCACCC CCTGCTGGAT TGAGATACAC CTGCATGGCC CTCTCCAGTG GCTGGATAAA 
GTCCTTACCC AGATGGGTTC GCCCCACAAC CCTATTTCGT CGGTGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR?203?3p participates in the suppression of diabetes?associated osteogenesis in the jaw bone through targeting Smad1.
International journal of molecular medicine41(3)1595-1607(2018 Mar)
Tang Y,Zheng L,Zhou J,Chen Y,Yang L,Deng F,Hu Y

Actin cytoskeleton mediates BMP2-Smad signaling via calponin 1 in preosteoblast under simulated microgravity.
Biochimie138184-193(2017 Jul)
Xu H,Wu F,Zhang H,Yang C,Li K,Wang H,Yang H,Liu Y,Ding B,Tan Y,Yuan M,Li Y,Dai Z

Cx43- and Smad-Mediated TGF-?/ BMP Signaling Pathway Promotes Cartilage Differentiation of Bone Marrow Mesenchymal Stem Cells and Inhibits Osteoblast Differentiation.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology42(4)1277-1293(2017)
Zhang YD,Zhao SC,Zhu ZS,Wang YF,Liu JX,Zhang ZC,Xue F

Alteration of TGF-?-ALK-Smad signaling in hyperoxia-induced bronchopulmonary dysplasia model of newborn rats.
Experimental lung research42(7)354-364(2016 Sep)
Jin M,Lee J,Lee KY,Jin Z,Pak JH,Kim HS

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Smad1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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