Scn5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scn5a
Entrez Gene ID	25665
Full Name	sodium voltage-gated channel alpha subunit 5
Synonyms	Nav1.5,SCAL
General protein information	Preferred Names sodium voltage-gated channel alpha subunit 5 Names sodium channel protein type 5 subunit alpha sodium channel protein cardiac muscle subunit alpha sodium channel protein type V subunit alpha sodium channel, voltage-gated, type 5, alpha subunit sodium channel, voltage-gated, type V, alpha polypeptide sodium channel, voltage-gated, type V, alpha subunit voltage-gated sodium channel Nav1.5c voltage-gated sodium channel subunit alpha Nav1.5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	acts as a tetrodotoxin-resistant voltage gated sodium channel; may play a role in cardiac function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160162.1	NP_001153634.1	sodium channel protein type 5 subunit alpha isoform 2
XM_017595481.1	XP_017450970.1	sodium channel protein type 5 subunit alpha isoform X2
NM_013125.2	NP_037257.1	sodium channel protein type 5 subunit alpha isoform 1
XM_006244076.3	XP_006244138.1	sodium channel protein type 5 subunit alpha isoform X1
XM_017595482.1	XP_017450971.1	sodium channel protein type 5 subunit alpha isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	scn12ab	NP_001038588.1
Homo sapiens (human)	SCN5A	NP_932173.1
Pan troglodytes (chimpanzee)	SCN5A	XP_001171891.2
	LOC695085	XP_002808375.1
Canis lupus familiaris (dog)	SCN5A	NP_001002994.1
Danio rerio (zebrafish)	scn12aa	NP_001038387.1
Rattus norvegicus (Norway rat)	Scn5a	NP_037257.1
Bos taurus (cattle)	SCN5A	NP_776883.1
Mus musculus (house mouse)	Scn5a	NP_067519.2
Gallus gallus (chicken)	SCN5A	XP_001232818.3
Xenopus tropicalis (tropical clawed frog)	scn5a	XP_002932534.2

Gene Ontology
Bibliography

Related articles in PubMed

Imaging cardiac SCN5A using the novel F-18 radiotracer radiocaine.
Hooker JM, Strebl MG, Schroeder FA, Wey HY, Ambardekar AV, McKinsey TA, Schoenberger M
Scientific reports742136(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinically relevant cardiotoxicity of domperidone and metoclopramide corresponds to a rather potent and local anesthetic-like inhibition of cardiac Na channels including Nav1.5.
Title: Cardiotoxic Antiemetics Metoclopramide and Domperidone Block Cardiac Voltage-Gated Na+ Channels.

Nav1.5 N-terminal domain binding to alpha1-syntrophin increases membrane density of human Kir2.1, Kir2.2 and Nav1.5 channels
Title: Nav1.5 N-terminal domain binding to ?1-syntrophin increases membrane density of human Kir2.1, Kir2.2 and Nav1.5 channels.

Scn5a is expressed in jejunum and colon smooth muscle and it contributes to smooth muscle electrophysiology.
Title: Expression and function of the Scn5a-encoded voltage-gated sodium channel NaV 1.5 in the rat jejunum.

UBR3/6 regulate cardiomyocyte Nav 1.5 channel protein levels via the ubiquitin-proteasome pathway.
Title: Cardiac Nav 1.5 is modulated by ubiquitin protein ligase E3 component n-recognin UBR3 and 6.

As in the brain, epilepsy was associated with an enhanced expression of the neuronal isoform NaV1.1 in cardiomyocyte. Epilepsy was also associated with a lower INa activation threshold resulting in increased cell excitability.
Title: Prolongation of action potential duration and QT interval during epilepsy linked to increased contribution of neuronal sodium channels to cardiac late Na+ current: potential mechanism for sudden death in epilepsy.

The following Scn5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scn5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04606	NM_001160162.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa62328	XM_017595482.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa02072	NM_013125.2 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa62327	XM_017595481.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa31752	XM_006244076.3 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scn5a ( NM_001160162.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001153634.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa04606
Clone ID Related Accession (Same CDS sequence)	NM_001160162.1
Accession Version	NM_001160162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 5 subunit alpha isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M27902.1, AF353637.1, FM058340.1 and CV796639.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF353637.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCATACAC AACTGAATTT GTGGACCTGG GCAATGTCTC AGCCTTACGC 
ACCTTCCGAG TCCTCCGGGC CCTGAAAACT ATATCGGTCA TTTCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTCGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCGTAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCAGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGCGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGTGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GGAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC GGAGCTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGACC 
CCTGAGGACA GTTACTCCGA GGGCAGCACA GCTGACATGA CCAACACCGC CGACCTCCTG 
GAGCAAATCC CAGACCTTGG TGAGGACGTC AAGGACCCAG AGGACTGCTT TACTGAAGGC 
TGCGTCCGAC GCTGTCCCTG CTGCATGGTA GACACAACCC AGTCCCCAGG GAAGGTCTGG 
TGGCGATTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTCGA GACTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTCG AGGACATCTA CCTGGAGGAG 
CGGAAGACCA TCAAGGTTCT GCTGGAGTAC GCGGACAAGA TGTTCACCTA CGTCTTTGTG 
TTGGAGATGC TGCTCAAGTG GGTGGCCTAC GGCTTCAAGA AGTACTTCAC CAACGCCTGG 
TGCTGGCTGG ACTTCCTGAT TGTGGACGTC TCGCTGGTCA GCCTCGTGGC AAACACCTTA 
GGCTTCGCCG AAATGGGTCC CATCAAGTCA CTGAGGACAC TGCGTGCACT TCGACCCCTG 
AGGGCCTTGT CGAGATTTGA GGGCATGCGG GTGGTGGTCA ATGCGCTGGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAGTTCGGT AGGTGCATCA ACCAGACAGA AGGGGACCTG 
CCTCTGAACT ACACCATCGT GAACAACAAG AGTGAGTGCG AGTCCTTCAA CGTGACCGGA 
GAGTTGTACT GGACCAAGGT GAAGGTCAAC TTTGACAACG TGGGAGCCGG GTACCTGGCC 
CTCCTGCAGG TGGCGACATT TAAAGGCTGG ATGGACATCA TGTATGCGGC TGTGGACTCC 
AGAGGGTATG AGGAGCAGCC GCAGTGGGAA GACAACCTCT ACATGTACAT CTACTTTGTC 
GTCTTCATCA TCTTCGGCTC CTTCTTCACC CTCAACCTCT TCATCGGTGT CATCATTGAC 
AACTTCAACC AGCAGAAGAA AAAGTTAGGG GGCCAGGATA TCTTCATGAC GGAGGAGCAG 
AAGAAGTACT ACAATGCCAT GAAGAAGCTG GGCTCCAAGA AACCCCAGAA GCCCATCCCA 
CGGCCCTTGA ACAAGTACCA GGGTTTCATA TTCGACATTG TGACCAAGCA GGCCTTCGAT 
GTCACCATCA TGTTCCTCAT CTGTTTGAAC ATGGTGACCA TGATGGTGGA GACAGATGAC 
CAGAGCCCTG AGAAGGTCAA CATCTTGGCC AAGATCAACC TGCTCTTCGT GGCCATCTTC 
ACAGGCGAGT GTATTGTCAA GATGGCTGCC CTGCGCCACT ATTACTTCAC CAACAGCTGG 
AACATCTTCG ACTTTGTGGT GGTCATCCTC TCCATTGTTG GCACTGTCCT CTCCGACATC 
ATCCAGAAGT ACTTCTTCTC CCCGACACTC TTCCGGGTCA TCCGTCTGGC CAGGATCGGC 
CGCATCCTCA GGCTGATCCG CGGAGCCAAG GGGATTCGCA CGCTGCTCTT CGCCCTCATG 
ATGTCCCTGC CCGCCCTCTT CAACATCGGC CTCCTCCTCT TCCTCGTCAT GTTCATCTAC 
TCCATCTTCG GCATGGCCAA CTTCGCTTAC GTCAAGTGGG AGGCCGGCAT CGATGACATG 
TTCAACTTCC AGACCTTCGC CAACAGCATG CTGTGCCTGT TCCAGATCAC CACATCAGCC 
GGCTGGGACG GCCTCCTCAG CCCCATCCTC AACACGGGGC CTCCCTACTG CGACCCCAAC 
CTGCCCAACA GCAACGGCTC CCGGGGGAAC TGTGGGAGCC CGGCGGTGGG CATCCTCTTC 
TTCACCACCT ACATCATCAT CTCCTTCCTC ATCGTGGTCA ACATGTACAT CGCCATCATC 
CTCGAGAACT TCAGCGTGGC CACCGAGGAG AGCACAGAGC CCCTGAGCGA GGACGACTTC 
GACATGTTCT ATGAGATCTG GGAGAAGTTC GACCCGGAGG CCACCCAGTT CATTGAGTAT 
CTGGCCCTGT CCGACTTTGC AGATGCCTTG TCTGAGCCGC TCCGCATCGC CAAACCCAAC 
CAGATAAGCC TCATCAACAT GGATCTGCCC ATGGTGAGCG GAGACCGTAT CCACTGTATG 
GACATACTGT TCGCTTTCAC CAAGAGGGTG CTCGGCGAGT CTGGGGAGAT GGATGCCCTG 
AAGATCCAGA TGGAGGAGAA GTTCATGGCG GCCAACCCTT CCAAGATCTC CTACGAGCCC 
ATCACCACCA CCCTGAGGAG AAAGCACGAG GAGGTGTCGG CCACGGTCAT CCAGCGTGCC 
TTCCGGAGGC ACCTGCTGCA GCGCTCGGTG AAGCATGCCT CCTTTCTCTT CCGCCAGCAA 
GCGGGCGGCA GTGGCCTCTC CGACGAGGAT GCCCCTGAGC GGGAGGGCCT CATCGCCTAC 
ATGATGAATG GGAACTTCTC TCGGCGCAGT GCTCCGCTCT CCAGCTCCTC CATCTCCTCC 
ACGTCCTTCC CCCCGTCCTA CGACAGCGTC ACGAGAGCCA CCAGTGATAA CCTCCCGGTG 
CGTGCGTCTG ACTATAGCCG CAGCGAAGAT CTTGCAGACT TCCCTCCATC TCCAGATAGG 
GACCGAGAGT CTATCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153634.1
CDS	198..6098
Translation	MANLLLPRGTSSFRRFTRESLAAIEKRMAEKQARGGSATSQESREGLQEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGLVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERKSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRHPSAQGQPGPGASAPGYVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSYLLRPMVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGILRRRPKKPAALATHSQLPSCITAPRSPPPPEVEKVPPARKETRFEEDKRPGQGTPGDSEPVCVPIAVAESDTEDQEEDEENSLGTEEESSKQTPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQSPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGGSGLSDEDAPEREGLIAYMMNGNFSRRSAPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	NM_001160162.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160162.1

ORF Insert Sequence:

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCATACAC AACTGAATTT GTGGACCTGG GCAATGTCTC AGCCTTACGC 
ACCTTCCGAG TCCTCCGGGC CCTGAAAACT ATATCGGTCA TTTCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTCGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCGTAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCAGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGCGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGTGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GGAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC GGAGCTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGACC 
CCTGAGGACA GTTACTCCGA GGGCAGCACA GCTGACATGA CCAACACCGC CGACCTCCTG 
GAGCAAATCC CAGACCTTGG TGAGGACGTC AAGGACCCAG AGGACTGCTT TACTGAAGGC 
TGCGTCCGAC GCTGTCCCTG CTGCATGGTA GACACAACCC AGTCCCCAGG GAAGGTCTGG 
TGGCGATTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTCGA GACTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTCG AGGACATCTA CCTGGAGGAG 
CGGAAGACCA TCAAGGTTCT GCTGGAGTAC GCGGACAAGA TGTTCACCTA CGTCTTTGTG 
TTGGAGATGC TGCTCAAGTG GGTGGCCTAC GGCTTCAAGA AGTACTTCAC CAACGCCTGG 
TGCTGGCTGG ACTTCCTGAT TGTGGACGTC TCGCTGGTCA GCCTCGTGGC AAACACCTTA 
GGCTTCGCCG AAATGGGTCC CATCAAGTCA CTGAGGACAC TGCGTGCACT TCGACCCCTG 
AGGGCCTTGT CGAGATTTGA GGGCATGCGG GTGGTGGTCA ATGCGCTGGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGCGTGAACC TCTTCGCCGG GAAGTTCGGT AGGTGCATCA ACCAGACAGA AGGGGACCTG 
CCTCTGAACT ACACCATCGT GAACAACAAG AGTGAGTGCG AGTCCTTCAA CGTGACCGGA 
GAGTTGTACT GGACCAAGGT GAAGGTCAAC TTTGACAACG TGGGAGCCGG GTACCTGGCC 
CTCCTGCAGG TGGCGACATT TAAAGGCTGG ATGGACATCA TGTATGCGGC TGTGGACTCC 
AGAGGGTATG AGGAGCAGCC GCAGTGGGAA GACAACCTCT ACATGTACAT CTACTTTGTC 
GTCTTCATCA TCTTCGGCTC CTTCTTCACC CTCAACCTCT TCATCGGTGT CATCATTGAC 
AACTTCAACC AGCAGAAGAA AAAGTTAGGG GGCCAGGATA TCTTCATGAC GGAGGAGCAG 
AAGAAGTACT ACAATGCCAT GAAGAAGCTG GGCTCCAAGA AACCCCAGAA GCCCATCCCA 
CGGCCCTTGA ACAAGTACCA GGGTTTCATA TTCGACATTG TGACCAAGCA GGCCTTCGAT 
GTCACCATCA TGTTCCTCAT CTGTTTGAAC ATGGTGACCA TGATGGTGGA GACAGATGAC 
CAGAGCCCTG AGAAGGTCAA CATCTTGGCC AAGATCAACC TGCTCTTCGT GGCCATCTTC 
ACAGGCGAGT GTATTGTCAA GATGGCTGCC CTGCGCCACT ATTACTTCAC CAACAGCTGG 
AACATCTTCG ACTTTGTGGT GGTCATCCTC TCCATTGTTG GCACTGTCCT CTCCGACATC 
ATCCAGAAGT ACTTCTTCTC CCCGACACTC TTCCGGGTCA TCCGTCTGGC CAGGATCGGC 
CGCATCCTCA GGCTGATCCG CGGAGCCAAG GGGATTCGCA CGCTGCTCTT CGCCCTCATG 
ATGTCCCTGC CCGCCCTCTT CAACATCGGC CTCCTCCTCT TCCTCGTCAT GTTCATCTAC 
TCCATCTTCG GCATGGCCAA CTTCGCTTAC GTCAAGTGGG AGGCCGGCAT CGATGACATG 
TTCAACTTCC AGACCTTCGC CAACAGCATG CTGTGCCTGT TCCAGATCAC CACATCAGCC 
GGCTGGGACG GCCTCCTCAG CCCCATCCTC AACACGGGGC CTCCCTACTG CGACCCCAAC 
CTGCCCAACA GCAACGGCTC CCGGGGGAAC TGTGGGAGCC CGGCGGTGGG CATCCTCTTC 
TTCACCACCT ACATCATCAT CTCCTTCCTC ATCGTGGTCA ACATGTACAT CGCCATCATC 
CTCGAGAACT TCAGCGTGGC CACCGAGGAG AGCACAGAGC CCCTGAGCGA GGACGACTTC 
GACATGTTCT ATGAGATCTG GGAGAAGTTC GACCCGGAGG CCACCCAGTT CATTGAGTAT 
CTGGCCCTGT CCGACTTTGC AGATGCCTTG TCTGAGCCGC TCCGCATCGC CAAACCCAAC 
CAGATAAGCC TCATCAACAT GGATCTGCCC ATGGTGAGCG GAGACCGTAT CCACTGTATG 
GACATACTGT TCGCTTTCAC CAAGAGGGTG CTCGGCGAGT CTGGGGAGAT GGATGCCCTG 
AAGATCCAGA TGGAGGAGAA GTTCATGGCG GCCAACCCTT CCAAGATCTC CTACGAGCCC 
ATCACCACCA CCCTGAGGAG AAAGCACGAG GAGGTGTCGG CCACGGTCAT CCAGCGTGCC 
TTCCGGAGGC ACCTGCTGCA GCGCTCGGTG AAGCATGCCT CCTTTCTCTT CCGCCAGCAA 
GCGGGCGGCA GTGGCCTCTC CGACGAGGAT GCCCCTGAGC GGGAGGGCCT CATCGCCTAC 
ATGATGAATG GGAACTTCTC TCGGCGCAGT GCTCCGCTCT CCAGCTCCTC CATCTCCTCC 
ACGTCCTTCC CCCCGTCCTA CGACAGCGTC ACGAGAGCCA CCAGTGATAA CCTCCCGGTG 
CGTGCGTCTG ACTATAGCCG CAGCGAAGAT CTTGCAGACT TCCCTCCATC TCCAGATAGG 
GACCGAGAGT CTATCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( XM_017595482.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450971.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X3, mRNA.

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CloneID	ORa62328
Clone ID Related Accession (Same CDS sequence)	XM_017595482.1
Accession Version	XM_017595482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 5 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCGTATGT ATCAGAAAAC ATAAAGCTAG GCAATTTGTC GGCTCTTCGA 
ACTTTCAGAG TCCTGAGAGC TCTGAAAACC ATTTCAGTTA TTCCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTTGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCATAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCGGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGTGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGTGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GGAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC GGAGCTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGACC 
CCTGAGGACA GTTACTCCGA GGGCAGCACA GCTGACATGA CCAACACCGC CGACCTCCTG 
GAGCAAATCC CAGACCTTGG TGAGGACGTC AAGGACCCAG AGGACTGCTT TACTGAAGGC 
TGCGTCCGAC GCTGTCCCTG CTGCATGGTA GACACAACCC AGTCCCCAGG GAAGGTCTGG 
TGGCGATTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTCGA GACTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTCG AGGACATCTA CCTGGAGGAG 
CGGAAGACCA TCAAGGTTCT GCTGGAGTAC GCGGACAAGA TGTTCACCTA CGTCTTTGTG 
TTGGAGATGC TGCTCAAGTG GGTGGCCTAC GGCTTCAAGA AGTACTTCAC CAACGCCTGG 
TGCTGGCTGG ACTTCCTGAT TGTGGACGTC TCGCTGGTCA GCCTCGTGGC AAACACCTTA 
GGCTTCGCCG AAATGGGTCC CATCAAGTCA CTGAGGACAC TGCGTGCACT TCGACCCCTG 
AGGGCCTTGT CGAGATTTGA GGGCATGCGG GTGGTGGTCA ATGCGCTGGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGTGTGAACC TCTTCGCCGG GAAGTTCGGT AGGTGCATCA ACCAGACAGA AGGGGACCTG 
CCTCTGAACT ACACCATCGT GAACAACAAG AGTGAGTGCG AGTCCTTCAA CGTGACCGGA 
GAGTTGTACT GGACCAAGGT GAAGGTCAAC TTTGACAACG TGGGAGCCGG GTACCTGGCC 
CTCCTGCAGG TGGCGACATT TAAAGGCTGG ATGGACATCA TGTATGCGGC TGTGGACTCC 
AGAGGGTATG AGGAGCAGCC GCAGTGGGAA GACAACCTCT ACATGTACAT CTACTTTGTC 
GTCTTCATCA TCTTCGGCTC CTTCTTCACC CTCAACCTCT TCATCGGTGT CATCATTGAC 
AACTTCAACC AGCAGAAGAA AAAGTTAGGG GGCCAGGATA TCTTCATGAC GGAGGAGCAG 
AAGAAGTACT ACAATGCCAT GAAGAAGCTG GGCTCCAAGA AACCCCAGAA GCCCATCCCA 
CGGCCCTTGA ACAAGTACCA GGGTTTCATA TTCGACATTG TGACCAAGCA GGCCTTCGAT 
GTCACCATCA TGTTCCTCAT CTGTTTGAAC ATGGTGACCA TGATGGTGGA GACAGATGAC 
CAGAGCCCTG AGAAGGTCAA CATCTTGGCC AAGATCAACC TGCTCTTCGT GGCCATCTTC 
ACAGGCGAGT GTATTGTCAA GATGGCTGCC CTGCGCCACT ATTACTTCAC CAACAGCTGG 
AACATCTTCG ACTTTGTGGT GGTCATCCTC TCCATTGTTG GCACTGTCCT CTCCGACATC 
ATCCAGAAGT ACTTCTTCTC CCCGACACTC TTCCGGGTCA TCCGTCTGGC CAGGATCGGC 
CGCATCCTCA GGCTGATCCG CGGAGCCAAG GGGATTCGCA CGCTGCTCTT CGCCCTCATG 
ATGTCCCTGC CCGCCCTCTT CAACATCGGC CTCCTCCTCT TCCTCGTCAT GTTCATCTAC 
TCCATCTTCG GCATGGCCAA CTTCGCTTAC GTCAAGTGGG AGGCCGGCAT CGATGACATG 
TTCAACTTCC AGACCTTCGC CAACAGCATG CTGTGCCTGT TCCAGATCAC CACATCGGCC 
GGCTGGGACG GCCTCCTCAG CCCCATCCTC AACACGGGGC CTCCCTACTG CGACCCCAAC 
CTGCCCAACA GCAACGGCTC CCGGGGGAAC TGTGGGAGCC CGGCGGTGGG CATCCTCTTC 
TTCACCACCT ACATCATCAT CTCCTTCCTC ATCGTGGTCA ACATGTACAT CGCCATCATC 
CTCGAGAACT TCAGCGTGGC CACCGAGGAG AGCACAGAGC CCCTGAGCGA GGACGACTTC 
GACATGTTCT ATGAGATCTG GGAGAAGTTC GACCCGGAGG CCACCCAGTT CATTGAGTAT 
CTGGCCCTGT CCGACTTTGC AGATGCCTTG TCTGAGCCGC TCCGCATCGC CAAACCCAAC 
CAGATAAGCC TCATCAACAT GGATCTGCCC ATGGTGAGCG GAGACCGTAT CCACTGTATG 
GACATACTGT TCGCTTTCAC CAAGAGGGTG CTCGGCGAGT CTGGGGAGAT GGATGCCCTG 
AAGATCCAGA TGGAGGAGAA GTTCATGGCG GCCAACCCTT CCAAGATCTC CTACGAGCCC 
ATCACCACCA CCCTGAGGAG AAAGCACGAG GAGGTGTCGG CCACGGTCAT CCAGCGTGCC 
TTCCGGAGGC ACCTGCTGCA GCGCTCGGTG AAGCACGCCT CCTTTCTCTT CCGCCAGCAA 
GCGGGCGGCA GTGGCCTCTC CGATGAGGAT GCCCCTGAGC GGGAGGGCCT CATCGCCTAC 
ATGATGAATG GGAACTTCTC TCGGCGCAGT GCTCCGCTCT CCAGCTCCTC CATCTCCTCC 
ACGTCCTTCC CCCCGTCCTA CGACAGCGTC ACGAGAGCCA CCAGTGATAA CCTCCCGGTG 
CGTGCGTCTG ACTATAGCCG CAGCGAAGAT CTTGCAGACT TCCCTCCATC TCCAGATAGG 
GACCGAGAGT CTATCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450971.1
CDS	1..5901
Translation	MANLLLPRGTSSFRRFTRESLAAIEKRMAEKQARGGSATSQESREGLQEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYVSENIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGLVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSHSSLEMSPLAPVTNHERKSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRHPSAQGQPGPGASAPGYVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSYLLRPMVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGILRRRPKKPAALATHSQLPSCITAPRSPPPPEVEKVPPARKETRFEEDKRPGQGTPGDSEPVCVPIAVAESDTEDQEEDEENSLGTEEESSKQTPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQSPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGGSGLSDEDAPEREGLIAYMMNGNFSRRSAPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	XM_017595482.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595482.1

ORF Insert Sequence:

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCGTATGT ATCAGAAAAC ATAAAGCTAG GCAATTTGTC GGCTCTTCGA 
ACTTTCAGAG TCCTGAGAGC TCTGAAAACC ATTTCAGTTA TTCCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTTGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCATAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCGGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGTGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGTGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GGAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC GGAGCTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGACC 
CCTGAGGACA GTTACTCCGA GGGCAGCACA GCTGACATGA CCAACACCGC CGACCTCCTG 
GAGCAAATCC CAGACCTTGG TGAGGACGTC AAGGACCCAG AGGACTGCTT TACTGAAGGC 
TGCGTCCGAC GCTGTCCCTG CTGCATGGTA GACACAACCC AGTCCCCAGG GAAGGTCTGG 
TGGCGATTGC GCAAGACCTG CTACCGCATC GTGGAGCACA GCTGGTTCGA GACTTTCATC 
ATCTTCATGA TCCTGCTCAG CAGTGGAGCG CTGGCCTTCG AGGACATCTA CCTGGAGGAG 
CGGAAGACCA TCAAGGTTCT GCTGGAGTAC GCGGACAAGA TGTTCACCTA CGTCTTTGTG 
TTGGAGATGC TGCTCAAGTG GGTGGCCTAC GGCTTCAAGA AGTACTTCAC CAACGCCTGG 
TGCTGGCTGG ACTTCCTGAT TGTGGACGTC TCGCTGGTCA GCCTCGTGGC AAACACCTTA 
GGCTTCGCCG AAATGGGTCC CATCAAGTCA CTGAGGACAC TGCGTGCACT TCGACCCCTG 
AGGGCCTTGT CGAGATTTGA GGGCATGCGG GTGGTGGTCA ATGCGCTGGT GGGCGCCATC 
CCCTCCATCA TGAACGTCCT CCTCGTCTGC CTCATCTTCT GGCTCATCTT CAGCATCATG 
GGTGTGAACC TCTTCGCCGG GAAGTTCGGT AGGTGCATCA ACCAGACAGA AGGGGACCTG 
CCTCTGAACT ACACCATCGT GAACAACAAG AGTGAGTGCG AGTCCTTCAA CGTGACCGGA 
GAGTTGTACT GGACCAAGGT GAAGGTCAAC TTTGACAACG TGGGAGCCGG GTACCTGGCC 
CTCCTGCAGG TGGCGACATT TAAAGGCTGG ATGGACATCA TGTATGCGGC TGTGGACTCC 
AGAGGGTATG AGGAGCAGCC GCAGTGGGAA GACAACCTCT ACATGTACAT CTACTTTGTC 
GTCTTCATCA TCTTCGGCTC CTTCTTCACC CTCAACCTCT TCATCGGTGT CATCATTGAC 
AACTTCAACC AGCAGAAGAA AAAGTTAGGG GGCCAGGATA TCTTCATGAC GGAGGAGCAG 
AAGAAGTACT ACAATGCCAT GAAGAAGCTG GGCTCCAAGA AACCCCAGAA GCCCATCCCA 
CGGCCCTTGA ACAAGTACCA GGGTTTCATA TTCGACATTG TGACCAAGCA GGCCTTCGAT 
GTCACCATCA TGTTCCTCAT CTGTTTGAAC ATGGTGACCA TGATGGTGGA GACAGATGAC 
CAGAGCCCTG AGAAGGTCAA CATCTTGGCC AAGATCAACC TGCTCTTCGT GGCCATCTTC 
ACAGGCGAGT GTATTGTCAA GATGGCTGCC CTGCGCCACT ATTACTTCAC CAACAGCTGG 
AACATCTTCG ACTTTGTGGT GGTCATCCTC TCCATTGTTG GCACTGTCCT CTCCGACATC 
ATCCAGAAGT ACTTCTTCTC CCCGACACTC TTCCGGGTCA TCCGTCTGGC CAGGATCGGC 
CGCATCCTCA GGCTGATCCG CGGAGCCAAG GGGATTCGCA CGCTGCTCTT CGCCCTCATG 
ATGTCCCTGC CCGCCCTCTT CAACATCGGC CTCCTCCTCT TCCTCGTCAT GTTCATCTAC 
TCCATCTTCG GCATGGCCAA CTTCGCTTAC GTCAAGTGGG AGGCCGGCAT CGATGACATG 
TTCAACTTCC AGACCTTCGC CAACAGCATG CTGTGCCTGT TCCAGATCAC CACATCGGCC 
GGCTGGGACG GCCTCCTCAG CCCCATCCTC AACACGGGGC CTCCCTACTG CGACCCCAAC 
CTGCCCAACA GCAACGGCTC CCGGGGGAAC TGTGGGAGCC CGGCGGTGGG CATCCTCTTC 
TTCACCACCT ACATCATCAT CTCCTTCCTC ATCGTGGTCA ACATGTACAT CGCCATCATC 
CTCGAGAACT TCAGCGTGGC CACCGAGGAG AGCACAGAGC CCCTGAGCGA GGACGACTTC 
GACATGTTCT ATGAGATCTG GGAGAAGTTC GACCCGGAGG CCACCCAGTT CATTGAGTAT 
CTGGCCCTGT CCGACTTTGC AGATGCCTTG TCTGAGCCGC TCCGCATCGC CAAACCCAAC 
CAGATAAGCC TCATCAACAT GGATCTGCCC ATGGTGAGCG GAGACCGTAT CCACTGTATG 
GACATACTGT TCGCTTTCAC CAAGAGGGTG CTCGGCGAGT CTGGGGAGAT GGATGCCCTG 
AAGATCCAGA TGGAGGAGAA GTTCATGGCG GCCAACCCTT CCAAGATCTC CTACGAGCCC 
ATCACCACCA CCCTGAGGAG AAAGCACGAG GAGGTGTCGG CCACGGTCAT CCAGCGTGCC 
TTCCGGAGGC ACCTGCTGCA GCGCTCGGTG AAGCACGCCT CCTTTCTCTT CCGCCAGCAA 
GCGGGCGGCA GTGGCCTCTC CGATGAGGAT GCCCCTGAGC GGGAGGGCCT CATCGCCTAC 
ATGATGAATG GGAACTTCTC TCGGCGCAGT GCTCCGCTCT CCAGCTCCTC CATCTCCTCC 
ACGTCCTTCC CCCCGTCCTA CGACAGCGTC ACGAGAGCCA CCAGTGATAA CCTCCCGGTG 
CGTGCGTCTG ACTATAGCCG CAGCGAAGAT CTTGCAGACT TCCCTCCATC TCCAGATAGG 
GACCGAGAGT CTATCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( NM_013125.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037257.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant 1, mRNA.

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CloneID	ORa02072
Clone ID Related Accession (Same CDS sequence)	NM_013125.2
Accession Version	NM_013125.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 5 subunit alpha isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M27902.1, FM058340.1 and CV796639.1. On May 14, 2009 this sequence version replaced NM_013125.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M27902.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCATACAC AACTGAATTT GTGGACCTGG GCAATGTCTC AGCCTTACGC 
ACCTTCCGAG TCCTCCGGGC CCTGAAAACT ATATCGGTCA TTTCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTCGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCGTAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCGGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGCGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGCGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GAAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC AGAACTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGGAA 
TCCCAAGTTG TGTCTGGTGG CCACGAGCCC TACCAGGAGC CCAGGGCCTG GAGCCAGGTG 
TCAGAGACCA CGTCCTCTGA AGCTGGGGCC AGTACATCTC AGGCAGACTG GCAGCAAGAG 
CAGAAAACGG AGCCCCAGGC CCCGGGGTGC GGTGAGACCC CTGAGGACAG TTACTCCGAG 
GGCAGCACAG CTGACATGAC CAACACCGCC GACCTCCTGG AGCAAATCCC AGACCTTGGT 
GAGGACGTCA AGGACCCAGA GGACTGCTTT ACTGAAGGCT GCGTCCGACG CTGTCCCTGC 
TGCATGGTAG ACACAACCCA GTCCCCAGGG AAGGTCTGGT GGCGATTGCG CAAGACCTGC 
TACCGCATCG TGGAGCACAG CTGGTTCGAG ACTTTCATCA TCTTCATGAT CCTGCTCAGC 
AGTGGAGCGC TGGCCTTCGA GGACATCTAC CTGGAGGAGC GGAAGACCAT CAAGGTTCTG 
CTGGAGTACG CGGACAAGAT GTTCACCTAC GTCTTTGTGT TGGAGATGCT GCTCAAGTGG 
GTGGCCTACG GCTTCAAGAA GTACTTCACC AACGCCTGGT GCTGGCTGGA CTTCCTGATT 
GTGGACGTCT CGCTGGTCAG CCTCGTGGCA AACACCTTAG GCTTCGCCGA AATGGGTCCC 
ATCAAGTCAC TGAGGACACT GCGTGCACTT CGACCCCTGA GGGCCTTGTC GAGATTTGAG 
GGCATGCGGG TGGTGGTCAA TGCGCTGGTG GGCGCCATCC CCTCCATCAT GAACGTCCTC 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GCGTGAACCT CTTCGCCGGG 
AAGTTCGGTA GGTGCATCAA CCAGACAGAA GGGGACCTGC CTCTGAACTA CACCATCGTG 
AACAACAAGA GTGAGTGCGA GTCCTTCAAC GTGACCGGAG AGTTGTACTG GACCAAGGTG 
AAGGTCAACT TTGACAACGT GGGAGCCGGG TACCTGGCCC TCCTGCAGGT GGCGACATTT 
AAAGGCTGGA TGGACATCAT GTATGCGGCT GTGGACTCCA GAGGGTATGA GGAGCAGCCG 
CAGTGGGAAG ACAACCTCTA CATGTACATC TACTTTGTCG TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATCGGTGTC ATCATTGACA ACTTCAACCA GCAGAAGAAA 
AAGTTAGGGG GCCAGGATAT CTTCATGACG GAGGAGCAGA AGAAGTACTA CAATGCCATG 
AAGAAGCTGG GCTCCAAGAA ACCCCAGAAG CCCATCCCAC GGCCCTTGAA CAAGTACCAG 
GGTTTCATAT TCGACATTGT GACCAAGCAG GCCTTCGATG TCACCATCAT GTTCCTCATC 
TGTTTGAACA TGGTGACCAT GATGGTGGAG ACAGATGACC AGAGCCCTGA GAAGGTCAAC 
ATCTTGGCCA AGATCAACCT GCTCTTCGTG GCCATCTTCA CAGGCGAGTG TATTGTCAAG 
ATGGCTGCCC TGCGCCACTA TTACTTCACC AACAGCTGGA ACATCTTCGA CTTTGTGGTG 
GTCATCCTCT CCATTGTTGG CACTGTCCTC TCCGACATCA TCCAGAAGTA CTTCTTCTCC 
CCGACACTCT TCCGGGTCAT CCGTCTGGCC AGGATCGGCC GCATCCTCAG GCTGATCCGC 
GGAGCCAAGG GGATTCGCAC GCTGCTCTTC GCCCTCATGA TGTCCCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTCGTCATG TTCATCTACT CCATCTTCGG CATGGCCAAC 
TTCGCTTACG TCAAGTGGGA GGCCGGCATC GATGACATGT TCAACTTCCA GACCTTCGCC 
AACAGCATGC TGTGCCTGTT CCAGATCACC ACATCAGCCG GCTGGGACGG CCTCCTCAGC 
CCCATCCTCA ACACGGGGCC TCCCTACTGC GACCCCAACC TGCCCAACAG CAACGGCTCC 
CGGGGGAACT GTGGGAGCCC GGCGGTGGGC ATCCTCTTCT TCACCACCTA CATCATCATC 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TCGAGAACTT CAGCGTGGCC 
ACCGAGGAGA GCACAGAGCC CCTGAGCGAG GACGACTTCG ACATGTTCTA TGAGATCTGG 
GAGAAGTTCG ACCCGGAGGC CACCCAGTTC ATTGAGTATC TGGCCCTGTC CGACTTTGCA 
GATGCCTTGT CTGAGCCGCT CCGCATCGCC AAACCCAACC AGATAAGCCT CATCAACATG 
GATCTGCCCA TGGTGAGCGG AGACCGTATC CACTGTATGG ACATACTGTT CGCTTTCACC 
AAGAGGGTGC TCGGCGAGTC TGGGGAGATG GATGCCCTGA AGATCCAGAT GGAGGAGAAG 
TTCATGGCGG CCAACCCTTC CAAGATCTCC TACGAGCCCA TCACCACCAC CCTGAGGAGA 
AAGCACGAGG AGGTGTCGGC CACGGTCATC CAGCGTGCCT TCCGGAGGCA CCTGCTGCAG 
CGCTCGGTGA AGCATGCCTC CTTTCTCTTC CGCCAGCAAG CGGGCGGCAG TGGCCTCTCC 
GACGAGGATG CCCCTGAGCG GGAGGGCCTC ATCGCCTACA TGATGAATGG GAACTTCTCT 
CGGCGCAGTG CTCCGCTCTC CAGCTCCTCC ATCTCCTCCA CGTCCTTCCC CCCGTCCTAC 
GACAGCGTCA CGAGAGCCAC CAGTGATAAC CTCCCGGTGC GTGCGTCTGA CTATAGCCGC 
AGCGAAGATC TTGCAGACTT CCCTCCATCT CCAGATAGGG ACCGAGAGTC TATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037257.1
CDS	198..6257
Translation	MANLLLPRGTSSFRRFTRESLAAIEKRMAEKQARGGSATSQESREGLQEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGLVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERKSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRHPSAQGQPGPGASAPGYVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSYLLRPMVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGILRRRPKKPAALATHSQLPSCITAPRSPPPPEVEKVPPARKETRFEEDKRPGQGTPGDSEPVCVPIAVAESDTEDQEEDEENSLGTEEESSKQESQVVSGGHEPYQEPRAWSQVSETTSSEAGASTSQADWQQEQKTEPQAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQSPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGGSGLSDEDAPEREGLIAYMMNGNFSRRSAPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	NM_013125.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013125.2

ORF Insert Sequence:

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCATACAC AACTGAATTT GTGGACCTGG GCAATGTCTC AGCCTTACGC 
ACCTTCCGAG TCCTCCGGGC CCTGAAAACT ATATCGGTCA TTTCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTCGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCGTAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCGGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGCGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGCGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GAAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC AGAACTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGGAA 
TCCCAAGTTG TGTCTGGTGG CCACGAGCCC TACCAGGAGC CCAGGGCCTG GAGCCAGGTG 
TCAGAGACCA CGTCCTCTGA AGCTGGGGCC AGTACATCTC AGGCAGACTG GCAGCAAGAG 
CAGAAAACGG AGCCCCAGGC CCCGGGGTGC GGTGAGACCC CTGAGGACAG TTACTCCGAG 
GGCAGCACAG CTGACATGAC CAACACCGCC GACCTCCTGG AGCAAATCCC AGACCTTGGT 
GAGGACGTCA AGGACCCAGA GGACTGCTTT ACTGAAGGCT GCGTCCGACG CTGTCCCTGC 
TGCATGGTAG ACACAACCCA GTCCCCAGGG AAGGTCTGGT GGCGATTGCG CAAGACCTGC 
TACCGCATCG TGGAGCACAG CTGGTTCGAG ACTTTCATCA TCTTCATGAT CCTGCTCAGC 
AGTGGAGCGC TGGCCTTCGA GGACATCTAC CTGGAGGAGC GGAAGACCAT CAAGGTTCTG 
CTGGAGTACG CGGACAAGAT GTTCACCTAC GTCTTTGTGT TGGAGATGCT GCTCAAGTGG 
GTGGCCTACG GCTTCAAGAA GTACTTCACC AACGCCTGGT GCTGGCTGGA CTTCCTGATT 
GTGGACGTCT CGCTGGTCAG CCTCGTGGCA AACACCTTAG GCTTCGCCGA AATGGGTCCC 
ATCAAGTCAC TGAGGACACT GCGTGCACTT CGACCCCTGA GGGCCTTGTC GAGATTTGAG 
GGCATGCGGG TGGTGGTCAA TGCGCTGGTG GGCGCCATCC CCTCCATCAT GAACGTCCTC 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GCGTGAACCT CTTCGCCGGG 
AAGTTCGGTA GGTGCATCAA CCAGACAGAA GGGGACCTGC CTCTGAACTA CACCATCGTG 
AACAACAAGA GTGAGTGCGA GTCCTTCAAC GTGACCGGAG AGTTGTACTG GACCAAGGTG 
AAGGTCAACT TTGACAACGT GGGAGCCGGG TACCTGGCCC TCCTGCAGGT GGCGACATTT 
AAAGGCTGGA TGGACATCAT GTATGCGGCT GTGGACTCCA GAGGGTATGA GGAGCAGCCG 
CAGTGGGAAG ACAACCTCTA CATGTACATC TACTTTGTCG TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATCGGTGTC ATCATTGACA ACTTCAACCA GCAGAAGAAA 
AAGTTAGGGG GCCAGGATAT CTTCATGACG GAGGAGCAGA AGAAGTACTA CAATGCCATG 
AAGAAGCTGG GCTCCAAGAA ACCCCAGAAG CCCATCCCAC GGCCCTTGAA CAAGTACCAG 
GGTTTCATAT TCGACATTGT GACCAAGCAG GCCTTCGATG TCACCATCAT GTTCCTCATC 
TGTTTGAACA TGGTGACCAT GATGGTGGAG ACAGATGACC AGAGCCCTGA GAAGGTCAAC 
ATCTTGGCCA AGATCAACCT GCTCTTCGTG GCCATCTTCA CAGGCGAGTG TATTGTCAAG 
ATGGCTGCCC TGCGCCACTA TTACTTCACC AACAGCTGGA ACATCTTCGA CTTTGTGGTG 
GTCATCCTCT CCATTGTTGG CACTGTCCTC TCCGACATCA TCCAGAAGTA CTTCTTCTCC 
CCGACACTCT TCCGGGTCAT CCGTCTGGCC AGGATCGGCC GCATCCTCAG GCTGATCCGC 
GGAGCCAAGG GGATTCGCAC GCTGCTCTTC GCCCTCATGA TGTCCCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTCGTCATG TTCATCTACT CCATCTTCGG CATGGCCAAC 
TTCGCTTACG TCAAGTGGGA GGCCGGCATC GATGACATGT TCAACTTCCA GACCTTCGCC 
AACAGCATGC TGTGCCTGTT CCAGATCACC ACATCAGCCG GCTGGGACGG CCTCCTCAGC 
CCCATCCTCA ACACGGGGCC TCCCTACTGC GACCCCAACC TGCCCAACAG CAACGGCTCC 
CGGGGGAACT GTGGGAGCCC GGCGGTGGGC ATCCTCTTCT TCACCACCTA CATCATCATC 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TCGAGAACTT CAGCGTGGCC 
ACCGAGGAGA GCACAGAGCC CCTGAGCGAG GACGACTTCG ACATGTTCTA TGAGATCTGG 
GAGAAGTTCG ACCCGGAGGC CACCCAGTTC ATTGAGTATC TGGCCCTGTC CGACTTTGCA 
GATGCCTTGT CTGAGCCGCT CCGCATCGCC AAACCCAACC AGATAAGCCT CATCAACATG 
GATCTGCCCA TGGTGAGCGG AGACCGTATC CACTGTATGG ACATACTGTT CGCTTTCACC 
AAGAGGGTGC TCGGCGAGTC TGGGGAGATG GATGCCCTGA AGATCCAGAT GGAGGAGAAG 
TTCATGGCGG CCAACCCTTC CAAGATCTCC TACGAGCCCA TCACCACCAC CCTGAGGAGA 
AAGCACGAGG AGGTGTCGGC CACGGTCATC CAGCGTGCCT TCCGGAGGCA CCTGCTGCAG 
CGCTCGGTGA AGCATGCCTC CTTTCTCTTC CGCCAGCAAG CGGGCGGCAG TGGCCTCTCC 
GACGAGGATG CCCCTGAGCG GGAGGGCCTC ATCGCCTACA TGATGAATGG GAACTTCTCT 
CGGCGCAGTG CTCCGCTCTC CAGCTCCTCC ATCTCCTCCA CGTCCTTCCC CCCGTCCTAC 
GACAGCGTCA CGAGAGCCAC CAGTGATAAC CTCCCGGTGC GTGCGTCTGA CTATAGCCGC 
AGCGAAGATC TTGCAGACTT CCCTCCATCT CCAGATAGGG ACCGAGAGTC TATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( XM_017595481.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450970.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X2, mRNA.

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CloneID	ORa62327
Clone ID Related Accession (Same CDS sequence)	XM_017595481.1
Accession Version	XM_017595481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 5 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCGTATGT ATCAGAAAAC ATAAAGCTAG GCAATTTGTC GGCTCTTCGA 
ACTTTCAGAG TCCTGAGAGC TCTGAAAACC ATTTCAGTTA TTCCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTTGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCATAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCGGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGTGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGTGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GGAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC GGAGCTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGGAA 
TCCCAAGTTG TGTCTGGTGG CCACGAGCCC TACCAGGAGC CCAGGGCCTG GAGCCAGGTG 
TCAGAGACCA CGTCCTCTGA AGCTGGGGCC AGTACATCTC AGGCAGACTG GCAGCAAGAG 
CAGAAAACGG AGCCCCAGGC CCCGGGGTGC GGTGAGACCC CTGAGGACAG TTACTCCGAG 
GGCAGCACAG CTGACATGAC CAACACCGCC GACCTCCTGG AGCAAATCCC AGACCTTGGT 
GAGGACGTCA AGGACCCAGA GGACTGCTTT ACTGAAGGCT GCGTCCGACG CTGTCCCTGC 
TGCATGGTAG ACACAACCCA GTCCCCAGGG AAGGTCTGGT GGCGATTGCG CAAGACCTGC 
TACCGCATCG TGGAGCACAG CTGGTTCGAG ACTTTCATCA TCTTCATGAT CCTGCTCAGC 
AGTGGAGCGC TGGCCTTCGA GGACATCTAC CTGGAGGAGC GGAAGACCAT CAAGGTTCTG 
CTGGAGTACG CGGACAAGAT GTTCACCTAC GTCTTTGTGT TGGAGATGCT GCTCAAGTGG 
GTGGCCTACG GCTTCAAGAA GTACTTCACC AACGCCTGGT GCTGGCTGGA CTTCCTGATT 
GTGGACGTCT CGCTGGTCAG CCTCGTGGCA AACACCTTAG GCTTCGCCGA AATGGGTCCC 
ATCAAGTCAC TGAGGACACT GCGTGCACTT CGACCCCTGA GGGCCTTGTC GAGATTTGAG 
GGCATGCGGG TGGTGGTCAA TGCGCTGGTG GGCGCCATCC CCTCCATCAT GAACGTCCTC 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GTGTGAACCT CTTCGCCGGG 
AAGTTCGGTA GGTGCATCAA CCAGACAGAA GGGGACCTGC CTCTGAACTA CACCATCGTG 
AACAACAAGA GTGAGTGCGA GTCCTTCAAC GTGACCGGAG AGTTGTACTG GACCAAGGTG 
AAGGTCAACT TTGACAACGT GGGAGCCGGG TACCTGGCCC TCCTGCAGGT GGCGACATTT 
AAAGGCTGGA TGGACATCAT GTATGCGGCT GTGGACTCCA GAGGGTATGA GGAGCAGCCG 
CAGTGGGAAG ACAACCTCTA CATGTACATC TACTTTGTCG TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATCGGTGTC ATCATTGACA ACTTCAACCA GCAGAAGAAA 
AAGTTAGGGG GCCAGGATAT CTTCATGACG GAGGAGCAGA AGAAGTACTA CAATGCCATG 
AAGAAGCTGG GCTCCAAGAA ACCCCAGAAG CCCATCCCAC GGCCCTTGAA CAAGTACCAG 
GGTTTCATAT TCGACATTGT GACCAAGCAG GCCTTCGATG TCACCATCAT GTTCCTCATC 
TGTTTGAACA TGGTGACCAT GATGGTGGAG ACAGATGACC AGAGCCCTGA GAAGGTCAAC 
ATCTTGGCCA AGATCAACCT GCTCTTCGTG GCCATCTTCA CAGGCGAGTG TATTGTCAAG 
ATGGCTGCCC TGCGCCACTA TTACTTCACC AACAGCTGGA ACATCTTCGA CTTTGTGGTG 
GTCATCCTCT CCATTGTTGG CACTGTCCTC TCCGACATCA TCCAGAAGTA CTTCTTCTCC 
CCGACACTCT TCCGGGTCAT CCGTCTGGCC AGGATCGGCC GCATCCTCAG GCTGATCCGC 
GGAGCCAAGG GGATTCGCAC GCTGCTCTTC GCCCTCATGA TGTCCCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTCGTCATG TTCATCTACT CCATCTTCGG CATGGCCAAC 
TTCGCTTACG TCAAGTGGGA GGCCGGCATC GATGACATGT TCAACTTCCA GACCTTCGCC 
AACAGCATGC TGTGCCTGTT CCAGATCACC ACATCGGCCG GCTGGGACGG CCTCCTCAGC 
CCCATCCTCA ACACGGGGCC TCCCTACTGC GACCCCAACC TGCCCAACAG CAACGGCTCC 
CGGGGGAACT GTGGGAGCCC GGCGGTGGGC ATCCTCTTCT TCACCACCTA CATCATCATC 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TCGAGAACTT CAGCGTGGCC 
ACCGAGGAGA GCACAGAGCC CCTGAGCGAG GACGACTTCG ACATGTTCTA TGAGATCTGG 
GAGAAGTTCG ACCCGGAGGC CACCCAGTTC ATTGAGTATC TGGCCCTGTC CGACTTTGCA 
GATGCCTTGT CTGAGCCGCT CCGCATCGCC AAACCCAACC AGATAAGCCT CATCAACATG 
GATCTGCCCA TGGTGAGCGG AGACCGTATC CACTGTATGG ACATACTGTT CGCTTTCACC 
AAGAGGGTGC TCGGCGAGTC TGGGGAGATG GATGCCCTGA AGATCCAGAT GGAGGAGAAG 
TTCATGGCGG CCAACCCTTC CAAGATCTCC TACGAGCCCA TCACCACCAC CCTGAGGAGA 
AAGCACGAGG AGGTGTCGGC CACGGTCATC CAGCGTGCCT TCCGGAGGCA CCTGCTGCAG 
CGCTCGGTGA AGCACGCCTC CTTTCTCTTC CGCCAGCAAG CGGGCGGCAG TGGCCTCTCC 
GATGAGGATG CCCCTGAGCG GGAGGGCCTC ATCGCCTACA TGATGAATGG GAACTTCTCT 
CGGCGCAGTG CTCCGCTCTC CAGCTCCTCC ATCTCCTCCA CGTCCTTCCC CCCGTCCTAC 
GACAGCGTCA CGAGAGCCAC CAGTGATAAC CTCCCGGTGC GTGCGTCTGA CTATAGCCGC 
AGCGAAGATC TTGCAGACTT CCCTCCATCT CCAGATAGGG ACCGAGAGTC TATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450970.1
CDS	240..6299
Translation	MANLLLPRGTSSFRRFTRESLAAIEKRMAEKQARGGSATSQESREGLQEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYVSENIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGLVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSHSSLEMSPLAPVTNHERKSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRHPSAQGQPGPGASAPGYVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSYLLRPMVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGILRRRPKKPAALATHSQLPSCITAPRSPPPPEVEKVPPARKETRFEEDKRPGQGTPGDSEPVCVPIAVAESDTEDQEEDEENSLGTEEESSKQESQVVSGGHEPYQEPRAWSQVSETTSSEAGASTSQADWQQEQKTEPQAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQSPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGGSGLSDEDAPEREGLIAYMMNGNFSRRSAPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	XM_017595481.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595481.1

ORF Insert Sequence:

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCGTATGT ATCAGAAAAC ATAAAGCTAG GCAATTTGTC GGCTCTTCGA 
ACTTTCAGAG TCCTGAGAGC TCTGAAAACC ATTTCAGTTA TTCCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTTGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCATAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCGGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGTGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGTGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GGAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC GGAGCTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGGAA 
TCCCAAGTTG TGTCTGGTGG CCACGAGCCC TACCAGGAGC CCAGGGCCTG GAGCCAGGTG 
TCAGAGACCA CGTCCTCTGA AGCTGGGGCC AGTACATCTC AGGCAGACTG GCAGCAAGAG 
CAGAAAACGG AGCCCCAGGC CCCGGGGTGC GGTGAGACCC CTGAGGACAG TTACTCCGAG 
GGCAGCACAG CTGACATGAC CAACACCGCC GACCTCCTGG AGCAAATCCC AGACCTTGGT 
GAGGACGTCA AGGACCCAGA GGACTGCTTT ACTGAAGGCT GCGTCCGACG CTGTCCCTGC 
TGCATGGTAG ACACAACCCA GTCCCCAGGG AAGGTCTGGT GGCGATTGCG CAAGACCTGC 
TACCGCATCG TGGAGCACAG CTGGTTCGAG ACTTTCATCA TCTTCATGAT CCTGCTCAGC 
AGTGGAGCGC TGGCCTTCGA GGACATCTAC CTGGAGGAGC GGAAGACCAT CAAGGTTCTG 
CTGGAGTACG CGGACAAGAT GTTCACCTAC GTCTTTGTGT TGGAGATGCT GCTCAAGTGG 
GTGGCCTACG GCTTCAAGAA GTACTTCACC AACGCCTGGT GCTGGCTGGA CTTCCTGATT 
GTGGACGTCT CGCTGGTCAG CCTCGTGGCA AACACCTTAG GCTTCGCCGA AATGGGTCCC 
ATCAAGTCAC TGAGGACACT GCGTGCACTT CGACCCCTGA GGGCCTTGTC GAGATTTGAG 
GGCATGCGGG TGGTGGTCAA TGCGCTGGTG GGCGCCATCC CCTCCATCAT GAACGTCCTC 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GTGTGAACCT CTTCGCCGGG 
AAGTTCGGTA GGTGCATCAA CCAGACAGAA GGGGACCTGC CTCTGAACTA CACCATCGTG 
AACAACAAGA GTGAGTGCGA GTCCTTCAAC GTGACCGGAG AGTTGTACTG GACCAAGGTG 
AAGGTCAACT TTGACAACGT GGGAGCCGGG TACCTGGCCC TCCTGCAGGT GGCGACATTT 
AAAGGCTGGA TGGACATCAT GTATGCGGCT GTGGACTCCA GAGGGTATGA GGAGCAGCCG 
CAGTGGGAAG ACAACCTCTA CATGTACATC TACTTTGTCG TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATCGGTGTC ATCATTGACA ACTTCAACCA GCAGAAGAAA 
AAGTTAGGGG GCCAGGATAT CTTCATGACG GAGGAGCAGA AGAAGTACTA CAATGCCATG 
AAGAAGCTGG GCTCCAAGAA ACCCCAGAAG CCCATCCCAC GGCCCTTGAA CAAGTACCAG 
GGTTTCATAT TCGACATTGT GACCAAGCAG GCCTTCGATG TCACCATCAT GTTCCTCATC 
TGTTTGAACA TGGTGACCAT GATGGTGGAG ACAGATGACC AGAGCCCTGA GAAGGTCAAC 
ATCTTGGCCA AGATCAACCT GCTCTTCGTG GCCATCTTCA CAGGCGAGTG TATTGTCAAG 
ATGGCTGCCC TGCGCCACTA TTACTTCACC AACAGCTGGA ACATCTTCGA CTTTGTGGTG 
GTCATCCTCT CCATTGTTGG CACTGTCCTC TCCGACATCA TCCAGAAGTA CTTCTTCTCC 
CCGACACTCT TCCGGGTCAT CCGTCTGGCC AGGATCGGCC GCATCCTCAG GCTGATCCGC 
GGAGCCAAGG GGATTCGCAC GCTGCTCTTC GCCCTCATGA TGTCCCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTCGTCATG TTCATCTACT CCATCTTCGG CATGGCCAAC 
TTCGCTTACG TCAAGTGGGA GGCCGGCATC GATGACATGT TCAACTTCCA GACCTTCGCC 
AACAGCATGC TGTGCCTGTT CCAGATCACC ACATCGGCCG GCTGGGACGG CCTCCTCAGC 
CCCATCCTCA ACACGGGGCC TCCCTACTGC GACCCCAACC TGCCCAACAG CAACGGCTCC 
CGGGGGAACT GTGGGAGCCC GGCGGTGGGC ATCCTCTTCT TCACCACCTA CATCATCATC 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TCGAGAACTT CAGCGTGGCC 
ACCGAGGAGA GCACAGAGCC CCTGAGCGAG GACGACTTCG ACATGTTCTA TGAGATCTGG 
GAGAAGTTCG ACCCGGAGGC CACCCAGTTC ATTGAGTATC TGGCCCTGTC CGACTTTGCA 
GATGCCTTGT CTGAGCCGCT CCGCATCGCC AAACCCAACC AGATAAGCCT CATCAACATG 
GATCTGCCCA TGGTGAGCGG AGACCGTATC CACTGTATGG ACATACTGTT CGCTTTCACC 
AAGAGGGTGC TCGGCGAGTC TGGGGAGATG GATGCCCTGA AGATCCAGAT GGAGGAGAAG 
TTCATGGCGG CCAACCCTTC CAAGATCTCC TACGAGCCCA TCACCACCAC CCTGAGGAGA 
AAGCACGAGG AGGTGTCGGC CACGGTCATC CAGCGTGCCT TCCGGAGGCA CCTGCTGCAG 
CGCTCGGTGA AGCACGCCTC CTTTCTCTTC CGCCAGCAAG CGGGCGGCAG TGGCCTCTCC 
GATGAGGATG CCCCTGAGCG GGAGGGCCTC ATCGCCTACA TGATGAATGG GAACTTCTCT 
CGGCGCAGTG CTCCGCTCTC CAGCTCCTCC ATCTCCTCCA CGTCCTTCCC CCCGTCCTAC 
GACAGCGTCA CGAGAGCCAC CAGTGATAAC CTCCCGGTGC GTGCGTCTGA CTATAGCCGC 
AGCGAAGATC TTGCAGACTT CCCTCCATCT CCAGATAGGG ACCGAGAGTC TATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn5a ( XM_006244076.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244138.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X1, mRNA.

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CloneID	ORa31752
Clone ID Related Accession (Same CDS sequence)	XM_006244076.3
Accession Version	XM_006244076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 5 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCATACAC AACTGAATTT GTGGACCTGG GCAATGTCTC AGCCTTACGC 
ACCTTCCGAG TCCTCCGGGC CCTGAAAACT ATATCGGTCA TTTCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTTGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCATAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCGGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGTGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGTGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GGAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC GGAGCTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGGAA 
TCCCAAGTTG TGTCTGGTGG CCACGAGCCC TACCAGGAGC CCAGGGCCTG GAGCCAGGTG 
TCAGAGACCA CGTCCTCTGA AGCTGGGGCC AGTACATCTC AGGCAGACTG GCAGCAAGAG 
CAGAAAACGG AGCCCCAGGC CCCGGGGTGC GGTGAGACCC CTGAGGACAG TTACTCCGAG 
GGCAGCACAG CTGACATGAC CAACACCGCC GACCTCCTGG AGCAAATCCC AGACCTTGGT 
GAGGACGTCA AGGACCCAGA GGACTGCTTT ACTGAAGGCT GCGTCCGACG CTGTCCCTGC 
TGCATGGTAG ACACAACCCA GTCCCCAGGG AAGGTCTGGT GGCGATTGCG CAAGACCTGC 
TACCGCATCG TGGAGCACAG CTGGTTCGAG ACTTTCATCA TCTTCATGAT CCTGCTCAGC 
AGTGGAGCGC TGGCCTTCGA GGACATCTAC CTGGAGGAGC GGAAGACCAT CAAGGTTCTG 
CTGGAGTACG CGGACAAGAT GTTCACCTAC GTCTTTGTGT TGGAGATGCT GCTCAAGTGG 
GTGGCCTACG GCTTCAAGAA GTACTTCACC AACGCCTGGT GCTGGCTGGA CTTCCTGATT 
GTGGACGTCT CGCTGGTCAG CCTCGTGGCA AACACCTTAG GCTTCGCCGA AATGGGTCCC 
ATCAAGTCAC TGAGGACACT GCGTGCACTT CGACCCCTGA GGGCCTTGTC GAGATTTGAG 
GGCATGCGGG TGGTGGTCAA TGCGCTGGTG GGCGCCATCC CCTCCATCAT GAACGTCCTC 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GTGTGAACCT CTTCGCCGGG 
AAGTTCGGTA GGTGCATCAA CCAGACAGAA GGGGACCTGC CTCTGAACTA CACCATCGTG 
AACAACAAGA GTGAGTGCGA GTCCTTCAAC GTGACCGGAG AGTTGTACTG GACCAAGGTG 
AAGGTCAACT TTGACAACGT GGGAGCCGGG TACCTGGCCC TCCTGCAGGT GGCGACATTT 
AAAGGCTGGA TGGACATCAT GTATGCGGCT GTGGACTCCA GAGGGTATGA GGAGCAGCCG 
CAGTGGGAAG ACAACCTCTA CATGTACATC TACTTTGTCG TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATCGGTGTC ATCATTGACA ACTTCAACCA GCAGAAGAAA 
AAGTTAGGGG GCCAGGATAT CTTCATGACG GAGGAGCAGA AGAAGTACTA CAATGCCATG 
AAGAAGCTGG GCTCCAAGAA ACCCCAGAAG CCCATCCCAC GGCCCTTGAA CAAGTACCAG 
GGTTTCATAT TCGACATTGT GACCAAGCAG GCCTTCGATG TCACCATCAT GTTCCTCATC 
TGTTTGAACA TGGTGACCAT GATGGTGGAG ACAGATGACC AGAGCCCTGA GAAGGTCAAC 
ATCTTGGCCA AGATCAACCT GCTCTTCGTG GCCATCTTCA CAGGCGAGTG TATTGTCAAG 
ATGGCTGCCC TGCGCCACTA TTACTTCACC AACAGCTGGA ACATCTTCGA CTTTGTGGTG 
GTCATCCTCT CCATTGTTGG CACTGTCCTC TCCGACATCA TCCAGAAGTA CTTCTTCTCC 
CCGACACTCT TCCGGGTCAT CCGTCTGGCC AGGATCGGCC GCATCCTCAG GCTGATCCGC 
GGAGCCAAGG GGATTCGCAC GCTGCTCTTC GCCCTCATGA TGTCCCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTCGTCATG TTCATCTACT CCATCTTCGG CATGGCCAAC 
TTCGCTTACG TCAAGTGGGA GGCCGGCATC GATGACATGT TCAACTTCCA GACCTTCGCC 
AACAGCATGC TGTGCCTGTT CCAGATCACC ACATCGGCCG GCTGGGACGG CCTCCTCAGC 
CCCATCCTCA ACACGGGGCC TCCCTACTGC GACCCCAACC TGCCCAACAG CAACGGCTCC 
CGGGGGAACT GTGGGAGCCC GGCGGTGGGC ATCCTCTTCT TCACCACCTA CATCATCATC 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TCGAGAACTT CAGCGTGGCC 
ACCGAGGAGA GCACAGAGCC CCTGAGCGAG GACGACTTCG ACATGTTCTA TGAGATCTGG 
GAGAAGTTCG ACCCGGAGGC CACCCAGTTC ATTGAGTATC TGGCCCTGTC CGACTTTGCA 
GATGCCTTGT CTGAGCCGCT CCGCATCGCC AAACCCAACC AGATAAGCCT CATCAACATG 
GATCTGCCCA TGGTGAGCGG AGACCGTATC CACTGTATGG ACATACTGTT CGCTTTCACC 
AAGAGGGTGC TCGGCGAGTC TGGGGAGATG GATGCCCTGA AGATCCAGAT GGAGGAGAAG 
TTCATGGCGG CCAACCCTTC CAAGATCTCC TACGAGCCCA TCACCACCAC CCTGAGGAGA 
AAGCACGAGG AGGTGTCGGC CACGGTCATC CAGCGTGCCT TCCGGAGGCA CCTGCTGCAG 
CGCTCGGTGA AGCACGCCTC CTTTCTCTTC CGCCAGCAAG CGGGCGGCAG TGGCCTCTCC 
GATGAGGATG CCCCTGAGCG GGAGGGCCTC ATCGCCTACA TGATGAATGG GAACTTCTCT 
CGGCGCAGTG CTCCGCTCTC CAGCTCCTCC ATCTCCTCCA CGTCCTTCCC CCCGTCCTAC 
GACAGCGTCA CGAGAGCCAC CAGTGATAAC CTCCCGGTGC GTGCGTCTGA CTATAGCCGC 
AGCGAAGATC TTGCAGACTT CCCTCCATCT CCAGATAGGG ACCGAGAGTC TATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244138.1
CDS	361..6420
Translation	MANLLLPRGTSSFRRFTRESLAAIEKRMAEKQARGGSATSQESREGLQEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTELNGTNGSVEADGLVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSHSSLEMSPLAPVTNHERKSKRRKRLSSGTEDGGDDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTSLLVPWPLRHPSAQGQPGPGASAPGYVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSYLLRPMVLDRPPDTTTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGILRRRPKKPAALATHSQLPSCITAPRSPPPPEVEKVPPARKETRFEEDKRPGQGTPGDSEPVCVPIAVAESDTEDQEEDEENSLGTEEESSKQESQVVSGGHEPYQEPRAWSQVSETTSSEAGASTSQADWQQEQKTEPQAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQSPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGGSGLSDEDAPEREGLIAYMMNGNFSRRSAPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV

Target ORF information:

RefSeq Version	XM_006244076.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 5 (Scn5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244076.3

ORF Insert Sequence:

ATGGCAAACC TCCTGTTACC TCGGGGCACC AGCAGCTTCC GTAGGTTCAC CCGGGAGTCA 
CTGGCGGCCA TCGAGAAGCG AATGGCTGAA AAGCAAGCCC GAGGAGGTTC GGCCACCTCA 
CAGGAGAGCC GTGAGGGCCT GCAGGAGGAG GAGGCTCCCC GGCCCCAGCT GGACCTACAG 
GCCTCCAAAA AGCTGCCAGA TCTCTATGGC AACCCACCCC GAGAGCTCAT CGGGGAGCCC 
CTGGAAGACC TGGACCCTTT CTATAGTACC CAGAAGACCT TCATCGTGCT GAATAAGGGC 
AAAACCATCT TCCGGTTCAG TGCCACCAAT GCCTTGTATG TCCTCAGCCC CTTCCACCCC 
GTGCGCCGAG CGGCCGTGAA GATCCTGGTA CACTCGCTCT TTAGCATGCT CATCATGTGC 
ACCATCCTGA CCAACTGCGT GTTCATGGCC CAGCACGACC CTCCGCCTTG GACCAAATAT 
GTTGAGTACA CCTTCACTGC CATCTACACC TTTGAGTCTC TGGTCAAGAT TCTAGCTCGA 
GGCTTCTGCC TGCATGCATT CACCTTCCTT CGGGACCCGT GGAACTGGCT AGACTTCAGT 
GTGATCGTCA TGGCATACAC AACTGAATTT GTGGACCTGG GCAATGTCTC AGCCTTACGC 
ACCTTCCGAG TCCTCCGGGC CCTGAAAACT ATATCGGTCA TTTCAGGCCT GAAGACCATC 
GTGGGAGCCC TAATCCAGTC TGTGAAGAAA CTGGCCGATG TGATGGTCCT CACTGTCTTC 
TGCCTCAGTG TCTTTGCCCT CATTGGCCTG CAGCTCTTCA TGGGCAACCT GAGGCACAAG 
TGTGTGCGTA ACTTCACCGA GCTCAATGGC ACCAATGGTT CCGTGGAGGC CGACGGCCTA 
GTCTGGAACT CCCTGGACGT CTACCTCAAT GACCCAGCCA ATTACCTGCT CAAGAATGGC 
ACCACGGATG TGTTACTATG TGGGAACAGC TCTGATGCCG GGACATGCCC TGAGGGCTAT 
CGGTGCCTGA AGGCAGGTGA GAACCCAGAC CACGGTTACA CCAGCTTCGA CTCCTTCGCC 
TGGGCCTTCC TTGCACTCTT CCGCCTGATG ACACAGGACT GCTGGGAACG CCTATACCAG 
CAGACCCTGA GGTCCGCAGG AAAGATCTAC ATGATCTTCT TCATGCTTGT CATCTTTCTG 
GGCTCCTTCT ACCTGGTGAA CTTGATCCTG GCTGTGGTGG CCATGGCCTA CGAGGAGCAA 
AACCAAGCCA CCATCGCCGA GACGGAAGAG AAGGAGAAGC GCTTCCAGGA GGCCATGGAG 
ATGCTCAAGA AGGAACACGA GGCTCTCACC ATCAGGGGTG TGGATACCGT GTCCCATAGC 
TCTCTGGAGA TGTCTCCTTT GGCCCCAGTA ACCAACCATG AGAGAAAGAG CAAAAGGAGG 
AAACGACTAT CTTCGGGGAC AGAGGATGGT GGGGATGACA GGCTCCCCAA GTCGGACTCA 
GAAGATGGTC CCAGAGCATT GAATCAGCTC AGCCTCACCC ATGGGCTCAG CCGGACATCC 
ATGAGGCCCC GCTCGAGCCG AGGGAGCATT TTCACGTTCC GAAGACGGGA CCAAGGCTCT 
GAGGCGGACT TCGCAGATGA CGAGAACAGC ACTGCGGGGG AGAGTGAGAG CCACCGCACA 
TCGCTGCTGG TACCCTGGCC CCTGCGCCAT CCCAGCGCCC AAGGACAGCC CGGCCCTGGA 
GCCTCAGCTC CCGGTTACGT TCTCAATGGC AAAAGGAACA GCACCGTGGA CTGCAATGGG 
GTGGTTTCCT TGCTGGGGGC AGGTGACGCA GAGGCCACCT CCCCAGGGAG CTACCTTCTC 
CGCCCTATGG TGCTGGACCG ACCCCCAGAC ACGACCACTC CGTCAGAGGA GCCCGGTGGG 
CCCCAGATGC TGACACCTCA GGCTCCGTGT GCAGATGGTT TTGAGGAGCC CGGAGCACGG 
CAACGGGCAC TCAGCGCTGT CAGTGTCCTC ACCAGTGCCC TGGAAGAGTT GGAGGAGTCC 
CATCGGAAGT GTCCACCATG CTGGAACCGC TTTGCCCAGC ACTACCTCAT CTGGGAGTGC 
TGTCCACTCT GGATGTCCAT CAAGCAGAAG GTGAAGTTTG TGGTCATGGA CCCATTTGCC 
GACCTCACTA TCACCATGTG CATCGTGCTC AATACGCTCT TCATGGCTCT GGAGCATTAC 
AACATGACGG CAGAGTTTGA GGAGATGCTG CAGGTCGGAA ACCTGGTCTT CACGGGAATC 
TTCACAGCGG AGATGACCTT CAAGATCATC GCCCTTGACC CCTACTACTA CTTCCAGCAG 
GGCTGGAATA TCTTCGACAG CATCATCGTC ATCCTCAGTC TCATGGAGCT GGGGCTGTCC 
CGCATGGGCA ACTTGTCTGT GCTACGTTCC TTCCGTCTGC TGCGGGTCTT CAAGCTGGCC 
AAGTCCTGGC CCACCCTGAA CACGCTCATC AAGATCATCG GGAACTCCGT GGGCGCCCTG 
GGGAACCTGA CCCTGGTGCT GGCCATCATC GTCTTCATCT TCGCCGTGGT GGGCATGCAG 
CTCTTCGGCA AGAACTACTC GGAGCTGAGG CACCGCATCA GCGACTCCGG CCTGCTGCCC 
CGCTGGCACA TGATGGACTT TTTCCACGCC TTCCTCATCA TCTTCCGCAT CCTCTGTGGG 
GAGTGGATCG AGACCATGTG GGACTGCATG GAGGTGTCTG GGCAGTCGCT GTGCTTGCTG 
GTCTTCCTGC TCGTCATGGT CATTGGCAAC CTTGTGGTCC TGAATCTCTT CTTGGCCTTG 
CTGCTCAGCT CCTTCAGCGC AGACAACCTC ACAGCCCCTG ACGAGGATGG GGAGATGAAC 
AACCTCCAGC TGGCCCTGGC TCGCATCCAG AGGGGCCTGC GCTTTGTCAA GCGGACCACC 
TGGGACTTCT GCTGCGGGAT CCTGCGGCGG CGACCTAAGA AGCCCGCGGC TCTTGCCACC 
CACAGCCAGC TGCCCAGCTG TATCACCGCC CCCAGGTCCC CACCACCCCC AGAGGTGGAG 
AAGGTGCCCC CAGCCCGCAA GGAAACACGA TTCGAGGAGG ACAAGCGACC CGGCCAGGGC 
ACCCCTGGGG ATTCGGAGCC TGTGTGTGTG CCCATCGCCG TGGCTGAGTC AGACACTGAA 
GACCAGGAAG AGGATGAAGA GAACAGCCTT GGCACAGAGG AAGAGTCCAG CAAACAGGAA 
TCCCAAGTTG TGTCTGGTGG CCACGAGCCC TACCAGGAGC CCAGGGCCTG GAGCCAGGTG 
TCAGAGACCA CGTCCTCTGA AGCTGGGGCC AGTACATCTC AGGCAGACTG GCAGCAAGAG 
CAGAAAACGG AGCCCCAGGC CCCGGGGTGC GGTGAGACCC CTGAGGACAG TTACTCCGAG 
GGCAGCACAG CTGACATGAC CAACACCGCC GACCTCCTGG AGCAAATCCC AGACCTTGGT 
GAGGACGTCA AGGACCCAGA GGACTGCTTT ACTGAAGGCT GCGTCCGACG CTGTCCCTGC 
TGCATGGTAG ACACAACCCA GTCCCCAGGG AAGGTCTGGT GGCGATTGCG CAAGACCTGC 
TACCGCATCG TGGAGCACAG CTGGTTCGAG ACTTTCATCA TCTTCATGAT CCTGCTCAGC 
AGTGGAGCGC TGGCCTTCGA GGACATCTAC CTGGAGGAGC GGAAGACCAT CAAGGTTCTG 
CTGGAGTACG CGGACAAGAT GTTCACCTAC GTCTTTGTGT TGGAGATGCT GCTCAAGTGG 
GTGGCCTACG GCTTCAAGAA GTACTTCACC AACGCCTGGT GCTGGCTGGA CTTCCTGATT 
GTGGACGTCT CGCTGGTCAG CCTCGTGGCA AACACCTTAG GCTTCGCCGA AATGGGTCCC 
ATCAAGTCAC TGAGGACACT GCGTGCACTT CGACCCCTGA GGGCCTTGTC GAGATTTGAG 
GGCATGCGGG TGGTGGTCAA TGCGCTGGTG GGCGCCATCC CCTCCATCAT GAACGTCCTC 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GTGTGAACCT CTTCGCCGGG 
AAGTTCGGTA GGTGCATCAA CCAGACAGAA GGGGACCTGC CTCTGAACTA CACCATCGTG 
AACAACAAGA GTGAGTGCGA GTCCTTCAAC GTGACCGGAG AGTTGTACTG GACCAAGGTG 
AAGGTCAACT TTGACAACGT GGGAGCCGGG TACCTGGCCC TCCTGCAGGT GGCGACATTT 
AAAGGCTGGA TGGACATCAT GTATGCGGCT GTGGACTCCA GAGGGTATGA GGAGCAGCCG 
CAGTGGGAAG ACAACCTCTA CATGTACATC TACTTTGTCG TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATCGGTGTC ATCATTGACA ACTTCAACCA GCAGAAGAAA 
AAGTTAGGGG GCCAGGATAT CTTCATGACG GAGGAGCAGA AGAAGTACTA CAATGCCATG 
AAGAAGCTGG GCTCCAAGAA ACCCCAGAAG CCCATCCCAC GGCCCTTGAA CAAGTACCAG 
GGTTTCATAT TCGACATTGT GACCAAGCAG GCCTTCGATG TCACCATCAT GTTCCTCATC 
TGTTTGAACA TGGTGACCAT GATGGTGGAG ACAGATGACC AGAGCCCTGA GAAGGTCAAC 
ATCTTGGCCA AGATCAACCT GCTCTTCGTG GCCATCTTCA CAGGCGAGTG TATTGTCAAG 
ATGGCTGCCC TGCGCCACTA TTACTTCACC AACAGCTGGA ACATCTTCGA CTTTGTGGTG 
GTCATCCTCT CCATTGTTGG CACTGTCCTC TCCGACATCA TCCAGAAGTA CTTCTTCTCC 
CCGACACTCT TCCGGGTCAT CCGTCTGGCC AGGATCGGCC GCATCCTCAG GCTGATCCGC 
GGAGCCAAGG GGATTCGCAC GCTGCTCTTC GCCCTCATGA TGTCCCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTCGTCATG TTCATCTACT CCATCTTCGG CATGGCCAAC 
TTCGCTTACG TCAAGTGGGA GGCCGGCATC GATGACATGT TCAACTTCCA GACCTTCGCC 
AACAGCATGC TGTGCCTGTT CCAGATCACC ACATCGGCCG GCTGGGACGG CCTCCTCAGC 
CCCATCCTCA ACACGGGGCC TCCCTACTGC GACCCCAACC TGCCCAACAG CAACGGCTCC 
CGGGGGAACT GTGGGAGCCC GGCGGTGGGC ATCCTCTTCT TCACCACCTA CATCATCATC 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TCGAGAACTT CAGCGTGGCC 
ACCGAGGAGA GCACAGAGCC CCTGAGCGAG GACGACTTCG ACATGTTCTA TGAGATCTGG 
GAGAAGTTCG ACCCGGAGGC CACCCAGTTC ATTGAGTATC TGGCCCTGTC CGACTTTGCA 
GATGCCTTGT CTGAGCCGCT CCGCATCGCC AAACCCAACC AGATAAGCCT CATCAACATG 
GATCTGCCCA TGGTGAGCGG AGACCGTATC CACTGTATGG ACATACTGTT CGCTTTCACC 
AAGAGGGTGC TCGGCGAGTC TGGGGAGATG GATGCCCTGA AGATCCAGAT GGAGGAGAAG 
TTCATGGCGG CCAACCCTTC CAAGATCTCC TACGAGCCCA TCACCACCAC CCTGAGGAGA 
AAGCACGAGG AGGTGTCGGC CACGGTCATC CAGCGTGCCT TCCGGAGGCA CCTGCTGCAG 
CGCTCGGTGA AGCACGCCTC CTTTCTCTTC CGCCAGCAAG CGGGCGGCAG TGGCCTCTCC 
GATGAGGATG CCCCTGAGCG GGAGGGCCTC ATCGCCTACA TGATGAATGG GAACTTCTCT 
CGGCGCAGTG CTCCGCTCTC CAGCTCCTCC ATCTCCTCCA CGTCCTTCCC CCCGTCCTAC 
GACAGCGTCA CGAGAGCCAC CAGTGATAAC CTCCCGGTGC GTGCGTCTGA CTATAGCCGC 
AGCGAAGATC TTGCAGACTT CCCTCCATCT CCAGATAGGG ACCGAGAGTC TATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Imaging cardiac SCN5A using the novel F-18 radiotracer radiocaine.
Scientific reports742136(2017 Feb)
Hooker JM,Strebl MG,Schroeder FA,Wey HY,Ambardekar AV,McKinsey TA,Schoenberger M

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Scn5a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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