Gckr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gckr
Entrez Gene ID	25658
Full Name	glucokinase regulator
Synonyms	GLRE
General protein information	Preferred Names glucokinase regulator Names glucokinase regulatory protein glucokinase (hexokinase 4) regulator
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	inhibits the activity of glucokinase; plays a role in glucose metabolism and homeostasis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013120.2	NP_037252.1	glucokinase regulatory protein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gckr	NP_037252.1
Canis lupus familiaris (dog)	GCKR	XP_532912.3
Mus musculus (house mouse)	Gckr	NP_659158.1
	GCKR	XP_001098190.1
Homo sapiens (human)	GCKR	NP_001477.2
Pan troglodytes (chimpanzee)	GCKR	XP_515364.2
Bos taurus (cattle)	GCKR	XP_003586705.1
Danio rerio (zebrafish)	LOC100333044	XP_002665191.3
Xenopus tropicalis (tropical clawed frog)	LOC100493477	XP_002944386.2

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structure of glucokinase regulatory protein.
Pautsch A, Stadler N, L?hle A, Rist W, Berg A, Glocker L, Nar H, Reinert D, Lenter M, Heckel A, Schnapp G, Kauschke SG
Biochemistry52(20)3523-31(2013 May)

Modulation of glucokinase by glucose, small-molecule activator and glucokinase regulatory protein: steady-state kinetic and cell-based analysis.
Bourbonais FJ, Chen J, Huang C, Zhang Y, Pfefferkorn JA, Landro JA
The Biochemical journal441(3)881-7(2012 Feb)

Association of eGFR-Related Loci Identified by GWAS with Incident CKD and ESRD.
B?ger CA, Gorski M, Li M, Hoffmann MM, Huang C, Yang Q, Teumer A, Krane V, O'Seaghdha CM, Kutalik Z, Wichmann HE, Haak T, Boes E, Coassin S, Coresh J, Kollerits B, Haun M, Paulweber B, K?ttgen A, Li G, Shlipak MG, Powe N, Hwang SJ, Dehghan A, Rivadeneira F, Uitterlinden A, Hofman A, Beckmann JS, Kr?mer BK, Witteman J, Bochud M, Siscovick D, Rettig R, Kronenberg F, Wanner C, Thadhani RI, Heid IM, Fox CS, Kao WH,
PLoS genetics7(9)e1002292(2011 Sep)

Variants of GCKR affect both ?-cell and kidney function in patients with newly diagnosed type 2 diabetes: the Verona newly diagnosed type 2 diabetes study 2.
Bonetti S, Trombetta M, Boselli ML, Turrini F, Malerba G, Trabetti E, Pignatti PF, Bonora E, Bonadonna RC
Diabetes care34(5)1205-10(2011 May)

Effects of GCK, GCKR, G6PC2 and MTNR1B variants on glucose metabolism and insulin secretion.
Hu C, Zhang R, Wang C, Yu W, Lu J, Ma X, Wang J, Jiang F, Tang S, Bao Y, Xiang K, Jia W
PloS one5(7)e11761(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

When GK is pre-incubated with glucose and compound A, the inhibition observed by GKRP is time dependent, but independent of GKRP concentration, reflecting the GKA-controlled transition between closed and open GK conformations.
Title: Modulation of glucokinase by glucose, small-molecule activator and glucokinase regulatory protein: steady-state kinetic and cell-based analysis.

Our findings indicate that both glucokinase and glucokinase regulatory proteins may be functionally active before birth and that they can act within a glucose sensor system involved in controlling food intake.
Title: Glucokinase and glucokinase regulatory proteins are functionally coexpressed before birth in the rat brain.

Prototypical activator of Glucokinase (GK) of the amino-heteroaryl-amide type bound to GK in a glucose-dependent manner and impaired the association of GK with GK regulatory protein
Title: Biophysical characterization of the interaction between hepatic glucokinase and its regulatory protein: impact of physiological and pharmacological effectors.

mechanisms downstream of AMP-activated protein kinase activation, involving phosphorylation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and GKRP are involved in the ATP-independent inhibition of glucose-induced glucokinase translocation
Title: Inhibition of glucokinase translocation by AMP-activated protein kinase is associated with phosphorylation of both GKRP and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

Substrates and glucagon induced adaptive changes in the mitochondrial glucokinase/GKRP ratio suggesting a regulatory role for GKRP.
Title: Glucokinase regulatory protein is associated with mitochondria in hepatocytes.

The following Gckr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gckr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13470	NM_013120.2 Latest version!	Rattus norvegicus glucokinase regulator (Gckr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gckr ( NM_013120.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037252.1 Rattus norvegicus glucokinase regulator (Gckr), mRNA.

Price & Availability↑

CloneID	ORa13470
Clone ID Related Accession (Same CDS sequence)	NM_013120.2
Accession Version	NM_013120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	glucokinase regulatory protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081843.1. On Sep 16, 2004 this sequence version replaced NM_013120.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081843.1, X68497.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCAGGCA CCAAACGATA TCAGCATGTG ATCGAGACCC CTGAGCCTGG TGAATGGGAG 
TTGTCAGGGT ATGAAGCGGC TGTGCCAATC ACAGAGAAAT CCAACCCACT GACCCGAAAC 
CTGGACAAAG CAGATGCAGA GAAAATTGTC AAACTGCTGG GGCAGTGTGA TGCTGAGATA 
TTCCAGGAGG AGGGGCAGAT TGTGCCCACC TACCAGCGAC TATACAGCGA ATCAGTTCTG 
ACCACCATGT TGCAAGTGGC TGGAAAAGTC CAGGAAGTTC TGAAGGAGCC AGATGGGGGT 
CTGGTAGTGC TGAGTGGAGG GGGAACCTCT GGTCGTATGG CATTTCTCAT GTCTGTGTCT 
TTCAACCAGC TGATGAAAGG CCTGGGACAA AAGCCTCTTT ACACCTACCT CATTGCAGGA 
GGTGACAGGT CTGTTGTGGC CTCTCGTGAA CAGACAGAAG ATAGCGCCCT ACACGGGATC 
GAGGAGCTGA AGAAGGTGGC TGCTGGGAAG AAGAGAGTGG TCGTCATAGG CATCTCTGTG 
GGACTCTCTG CGCCCTTTGT GGCAGGTCAG ATGGACTACT GCATGGATAA CACAGCCGTC 
TTCTTGCCGG TTCTGGTTGG CTTCAATCCA GTGAGCATGG CCAGAAATGA CCCCATTGAA 
GACTGGAGAT CAACATTCCG GCAAGTGGCA GAGCGGATGC AAAAGATGCA GGAGAAACAG 
GAAGCTTTTG TGCTCAATCC TGCCATCGGG CCCGAGGGGC TCAGCGGCTC TTCCCGAATG 
AAAGGTGGAG GTGCCACCAA GATTCTACTG GAAACCCTGC TACTAGCAGC CCATAAGACT 
GTGGACCAGG GTGTTGTGTC CTCTCAAAGA TGCCTTCTGG AAATCCTGAG GACATTTGAG 
CGGGCTCATC AGGTGACCTA CAGTCAAAGT TCCAAAATTG CCACGCTGAT GAAACAAGTC 
GGCATCAGCC TGGAGAAGAA AGGCCGAGTG CACTTGGTTG GCTGGCAGAC TCTCGGCATC 
ATTGCCATTA TGGACGGAGT AGAGTGCATC CACACTTTTG GTGCTGATTT CCAAGATATC 
CGTGGCTTTC TTATTGGTGA CCACAGTGAC ATGTTTAACC AGAAGGATGA ACTCACCAAC 
CAGGGTCCCC AGTTCACCTT CTCCCAGGAT GACTTCCTGA CTTCCATCCT GCCATCCCTC 
ACGGAGACTG ACACCGTGGT CTTCATTTTT ACCCTGGATG ATAACCTCAC AGAAGTACAG 
GCCCTGGCAG AAAGAGTGAG AGAGAAGTGC CAGAACATCC AGGCCCTGGT GCACAGCACT 
GTGGGGCAGT CCTTGCCGGC CCCTCTAAAG AAACTCTTTC CCTCACTCAT CAGTATCACG 
TGGCCACTTC TTTTCTTCGA TTATGAAGGG ACCTATGTTC AGAAGTTCCA GCGTGAGTTA 
AGCACCAAGT GGGTGTTGAA TACAGTGAGT ACTGGGGCCC ATGTACTGCT GGGGAAGATC 
CTACAGAACC ACATGCTGGA CCTCCGCATC GCCAACTCCA AGCTCTTCTG GAGGGCGCTG 
GCCATGTTGC AGAGGTTCTC TGGACAGTCC AAGGCTCGCT GCATTGAGAG CCTCCTTCAA 
GCAATCCACT TTCCTCAACC ACTGTCGGAT GATGTCCGCG CCGCTCCCAT CTCCTGCCAC 
GTCCAGGTTG CCCACGAGAA GGAAAAGGTG ATCCCCACAG CCTTGCTGAG CCTCCTACTC 
CGGTGCTCCA TCTCTGAGGC TAAGGCACGC CTGTCTGCAG CTTCTTCAGT CTGTGAGGTT 
GTTAGGAGCG CCCTCTCTGG GCCGGGTCAG AAGCGCAGCA CGCAAGCCCT TGAAGACCCT 
CCCGCCTGTG GGACCCTGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037252.1
CDS	178..2061
Translation	MPGTKRYQHVIETPEPGEWELSGYEAAVPITEKSNPLTRNLDKADAEKIVKLLGQCDAEIFQEEGQIVPTYQRLYSESVLTTMLQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREQTEDSALHGIEELKKVAAGKKRVVVIGISVGLSAPFVAGQMDYCMDNTAVFLPVLVGFNPVSMARNDPIEDWRSTFRQVAERMQKMQEKQEAFVLNPAIGPEGLSGSSRMKGGGATKILLETLLLAAHKTVDQGVVSSQRCLLEILRTFERAHQVTYSQSSKIATLMKQVGISLEKKGRVHLVGWQTLGIIAIMDGVECIHTFGADFQDIRGFLIGDHSDMFNQKDELTNQGPQFTFSQDDFLTSILPSLTETDTVVFIFTLDDNLTEVQALAERVREKCQNIQALVHSTVGQSLPAPLKKLFPSLISITWPLLFFDYEGTYVQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRIANSKLFWRALAMLQRFSGQSKARCIESLLQAIHFPQPLSDDVRAAPISCHVQVAHEKEKVIPTALLSLLLRCSISEAKARLSAASSVCEVVRSALSGPGQKRSTQALEDPPACGTLN

Target ORF information:

RefSeq Version	NM_013120.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucokinase regulator (Gckr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013120.2

ORF Insert Sequence:

ATGCCAGGCA CCAAACGATA TCAGCATGTG ATCGAGACCC CTGAGCCTGG TGAATGGGAG 
TTGTCAGGGT ATGAAGCGGC TGTGCCAATC ACAGAGAAAT CCAACCCACT GACCCGAAAC 
CTGGACAAAG CAGATGCAGA GAAAATTGTC AAACTGCTGG GGCAGTGTGA TGCTGAGATA 
TTCCAGGAGG AGGGGCAGAT TGTGCCCACC TACCAGCGAC TATACAGCGA ATCAGTTCTG 
ACCACCATGT TGCAAGTGGC TGGAAAAGTC CAGGAAGTTC TGAAGGAGCC AGATGGGGGT 
CTGGTAGTGC TGAGTGGAGG GGGAACCTCT GGTCGTATGG CATTTCTCAT GTCTGTGTCT 
TTCAACCAGC TGATGAAAGG CCTGGGACAA AAGCCTCTTT ACACCTACCT CATTGCAGGA 
GGTGACAGGT CTGTTGTGGC CTCTCGTGAA CAGACAGAAG ATAGCGCCCT ACACGGGATC 
GAGGAGCTGA AGAAGGTGGC TGCTGGGAAG AAGAGAGTGG TCGTCATAGG CATCTCTGTG 
GGACTCTCTG CGCCCTTTGT GGCAGGTCAG ATGGACTACT GCATGGATAA CACAGCCGTC 
TTCTTGCCGG TTCTGGTTGG CTTCAATCCA GTGAGCATGG CCAGAAATGA CCCCATTGAA 
GACTGGAGAT CAACATTCCG GCAAGTGGCA GAGCGGATGC AAAAGATGCA GGAGAAACAG 
GAAGCTTTTG TGCTCAATCC TGCCATCGGG CCCGAGGGGC TCAGCGGCTC TTCCCGAATG 
AAAGGTGGAG GTGCCACCAA GATTCTACTG GAAACCCTGC TACTAGCAGC CCATAAGACT 
GTGGACCAGG GTGTTGTGTC CTCTCAAAGA TGCCTTCTGG AAATCCTGAG GACATTTGAG 
CGGGCTCATC AGGTGACCTA CAGTCAAAGT TCCAAAATTG CCACGCTGAT GAAACAAGTC 
GGCATCAGCC TGGAGAAGAA AGGCCGAGTG CACTTGGTTG GCTGGCAGAC TCTCGGCATC 
ATTGCCATTA TGGACGGAGT AGAGTGCATC CACACTTTTG GTGCTGATTT CCAAGATATC 
CGTGGCTTTC TTATTGGTGA CCACAGTGAC ATGTTTAACC AGAAGGATGA ACTCACCAAC 
CAGGGTCCCC AGTTCACCTT CTCCCAGGAT GACTTCCTGA CTTCCATCCT GCCATCCCTC 
ACGGAGACTG ACACCGTGGT CTTCATTTTT ACCCTGGATG ATAACCTCAC AGAAGTACAG 
GCCCTGGCAG AAAGAGTGAG AGAGAAGTGC CAGAACATCC AGGCCCTGGT GCACAGCACT 
GTGGGGCAGT CCTTGCCGGC CCCTCTAAAG AAACTCTTTC CCTCACTCAT CAGTATCACG 
TGGCCACTTC TTTTCTTCGA TTATGAAGGG ACCTATGTTC AGAAGTTCCA GCGTGAGTTA 
AGCACCAAGT GGGTGTTGAA TACAGTGAGT ACTGGGGCCC ATGTACTGCT GGGGAAGATC 
CTACAGAACC ACATGCTGGA CCTCCGCATC GCCAACTCCA AGCTCTTCTG GAGGGCGCTG 
GCCATGTTGC AGAGGTTCTC TGGACAGTCC AAGGCTCGCT GCATTGAGAG CCTCCTTCAA 
GCAATCCACT TTCCTCAACC ACTGTCGGAT GATGTCCGCG CCGCTCCCAT CTCCTGCCAC 
GTCCAGGTTG CCCACGAGAA GGAAAAGGTG ATCCCCACAG CCTTGCTGAG CCTCCTACTC 
CGGTGCTCCA TCTCTGAGGC TAAGGCACGC CTGTCTGCAG CTTCTTCAGT CTGTGAGGTT 
GTTAGGAGCG CCCTCTCTGG GCCGGGTCAG AAGCGCAGCA CGCAAGCCCT TGAAGACCCT 
CCCGCCTGTG GGACCCTGAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structure of glucokinase regulatory protein.
Biochemistry52(20)3523-31(2013 May)
Pautsch A,Stadler N,L?hle A,Rist W,Berg A,Glocker L,Nar H,Reinert D,Lenter M,Heckel A,Schnapp G,Kauschke SG

Modulation of glucokinase by glucose, small-molecule activator and glucokinase regulatory protein: steady-state kinetic and cell-based analysis.
The Biochemical journal441(3)881-7(2012 Feb)
Bourbonais FJ,Chen J,Huang C,Zhang Y,Pfefferkorn JA,Landro JA

Association of eGFR-Related Loci Identified by GWAS with Incident CKD and ESRD.
PLoS genetics7(9)e1002292(2011 Sep)
B?ger CA,Gorski M,Li M,Hoffmann MM,Huang C,Yang Q,Teumer A,Krane V,O'Seaghdha CM,Kutalik Z,Wichmann HE,Haak T,Boes E,Coassin S,Coresh J,Kollerits B,Haun M,Paulweber B,K?ttgen A,Li G,Shlipak MG,Powe N,Hwang SJ,Dehghan A,Rivadeneira F,Uitterlinden A,Hofman A,Beckmann JS,Kr?mer BK,Witteman J,Bochud M,Siscovick D,Rettig R,Kronenberg F,Wanner C,Thadhani RI,Heid IM,Fox CS,Kao WH,

Variants of GCKR affect both ?-cell and kidney function in patients with newly diagnosed type 2 diabetes: the Verona newly diagnosed type 2 diabetes study 2.
Diabetes care34(5)1205-10(2011 May)
Bonetti S,Trombetta M,Boselli ML,Turrini F,Malerba G,Trabetti E,Pignatti PF,Bonora E,Bonadonna RC

Effects of GCK, GCKR, G6PC2 and MTNR1B variants on glucose metabolism and insulin secretion.
PloS one5(7)e11761(2010 Jul)
Hu C,Zhang R,Wang C,Yu W,Lu J,Ma X,Wang J,Jiang F,Tang S,Bao Y,Xiang K,Jia W

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Gckr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報