Pde4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde4a
Entrez Gene ID	25638
Full Name	phosphodiesterase 4A
Synonyms	PHOSA
General protein information	Preferred Names phosphodiesterase 4A Names cAMP-specific 3',5'-cyclic phosphodiesterase 4A DPDE2 phosphodiesterase 4A, cAMP specific phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q13
Summary	human homolog catalyzes the hydrolysis of cAMP; activity is inhibited by rolipram [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013101.3	NP_037233.3	cAMP-specific 3',5'-cyclic phosphodiesterase 4A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pde4a	NP_037233.3
Danio rerio (zebrafish)	pde4a	XP_700812.5
Homo sapiens (human)	PDE4A	NP_001104777.1
Canis lupus familiaris (dog)	PDE4A	XP_003432829.2
Mus musculus (house mouse)	Pde4a	NP_899668.1
Xenopus tropicalis (tropical clawed frog)	LOC100488846	XP_004918913.1

Gene Ontology
Bibliography

Related articles in PubMed

Compartmentalized PDE4A5 Signaling Impairs Hippocampal Synaptic Plasticity and Long-Term Memory.
Havekes R, Park AJ, Tolentino RE, Bruinenberg VM, Tudor JC, Lee Y, Hansen RT, Guercio LA, Linton E, Neves-Zaph SR, Meerlo P, Baillie GS, Houslay MD, Abel T
The Journal of neuroscience : the official journal of the Society for Neuroscience36(34)8936-46(2016 08)

Rolipram attenuates bile duct ligation-induced liver injury in rats: a potential pathogenic role of PDE4.
Gobejishvili L, Barve S, Breitkopf-Heinlein K, Li Y, Zhang J, Avila DV, Dooley S, McClain CJ
The Journal of pharmacology and experimental therapeutics347(1)80-90(2013 Oct)

ERK regulation of phosphodiesterase 4 enhances dopamine-stimulated AMPA receptor membrane insertion.
Song RS, Massenburg B, Wenderski W, Jayaraman V, Thompson L, Neves SR
Proceedings of the National Academy of Sciences of the United States of America110(38)15437-42(2013 Sep)

Phosphoinositide 3-kinase ? protects against catecholamine-induced ventricular arrhythmia through protein kinase A-mediated regulation of distinct phosphodiesterases.
Ghigo A, Perino A, Mehel H, Zahradn?kov? A Jr, Morello F, Leroy J, Nikolaev VO, Damilano F, Cimino J, De Luca E, Richter W, Westenbroek R, Catterall WA, Zhang J, Yan C, Conti M, Gomez AM, Vandecasteele G, Hirsch E, Fischmeister R
Circulation126(17)2073-83(2012 Oct)

?-Adrenergic cAMP signals are predominantly regulated by phosphodiesterase type 4 in cultured adult rat aortic smooth muscle cells.
Zhai K, Hubert F, Nicolas V, Ji G, Fischmeister R, Leblais V
PloS one7(10)e47826(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PDE4A upregulation during cholestatic liver injury plays a role in inflammation and fibrosis.
Title: Rolipram attenuates bile duct ligation-induced liver injury in rats: a potential pathogenic role of PDE4.

ERK regulation of phosphodiesterase 4 enhances dopamine-stimulated AMPA receptor membrane insertion.
Title: ERK regulation of phosphodiesterase 4 enhances dopamine-stimulated AMPA receptor membrane insertion.

beta-adrenergic cAMP signals are predominantly regulated by phosphodiesterase type 4 in cultured adult rat aortic smooth muscle cells.
Title: ?-Adrenergic cAMP signals are predominantly regulated by phosphodiesterase type 4 in cultured adult rat aortic smooth muscle cells.

These results indicate that PDE4A and PLAT may be susceptibility genes for schizophrenia in the Japanese population.
Title: Positive association of phencyclidine-responsive genes, PDE4A and PLAT, with schizophrenia.

cGMP and cAMP phosphodiesterase PDE4 and AMPK/NOX2 have a role in early cardiac hypertrophy induced by angiotensin II
Title: Concerted regulation of cGMP and cAMP phosphodiesterases in early cardiac hypertrophy induced by angiotensin II.

The following Pde4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12542	NM_013101.3 Latest version!	Rattus norvegicus phosphodiesterase 4A (Pde4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde4a ( NM_013101.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037233.3 Rattus norvegicus phosphodiesterase 4A (Pde4a), mRNA.

Price & Availability↑

CloneID	ORa12542
Clone ID Related Accession (Same CDS sequence)	NM_013101.3
Accession Version	NM_013101.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cAMP-specific 3',5'-cyclic phosphodiesterase 4A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L27057.1. On Oct 21, 2007 this sequence version replaced NM_013101.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27057.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCTC CGGCCGCCCC CTCGGAAAGG AGCCTGTCTC TCTCTCTTCC GGGGCCCCGG 
GAGGGCCAGG CCACCCTGAA GCCGCCCCCC CAGCACCTGT GGCGGCAGCC GAGGACCCCG 
ATCCGCATCC AGCAACGCGG CTACCCGGAC AGTGCCGAGC GCTCAGAGAC AGAGCGCTCA 
CCGCACCGGC CCATAGAGCG CGCCGACGCC GTGGACACTG GCGACCGGCC AGGCCTGCGG 
ACTACCCGCA TGTCCTGGCC CTCGTCCTTC CACGGCACCG GTACCGGCGG AGGCAGCAGT 
AGGCGCTTGG AGGCAGAAAA TGGGCCAACG CCATCCCCTG GCCGCAGCCC CCTGGACTCG 
CAGGCGAGCC CGGGGCTTGT GCTGCATGCT GGGGCCACCA CCAGCCAGCG CCGCGAGTCC 
TTCCTCTACC GCTCAGACAG CGACTATGAC ATGTCACCGA AGGCTGTGTC CAGGAGCTCG 
TCTGTCGCCA GCGAAGCGCA CGCTGAGGAC CTCATTGTGA CACCATTTGC CCAGGTGCTG 
GCCAGTCTCC GCAGCGTTCG AAGCAACTTC TCACTCTTAA CCAATGTGCC CATCCCCAGC 
AACAAGAGGT CTCCACTGGG TGGCCCACCC TCTGTCTGCA AGGCCACACT GTCAGAGGAG 
ACGTGCCAGC AGCTGGCCCG GGAGACCCTG GAAGAGCTGG ATTGGTGCCT GGAGCAGCTG 
GAGACCATGC AGACCTACCG CTCTGTCAGC GAGATGGCCT CACACAAGTT CAAAAGGATG 
CTGAACCGTG AGCTCACACA CCTGTCGGAA ATGAGCAGGT CAGGAAACCA GGTCTCAGAG 
TACATTTCCA ACACATTCCT GGACAAGCAG AATGAAGTGG AGATCCCCTC ACCCACACCT 
CGGCAGAGAG CCTTCCAGCA GCCCCCGCCG TCAGTGCTGC GACAGTCCCA GCCCATGTCT 
CAGATCACAG GGCTGAAAAA GCTGGTACAC ACTGGAAGCT TGAACACCAA CGTCCCACGG 
TTTGGAGTCA AGACAGATCA AGAGGACCTC TTAGCACAAG AACTGGAGAA CTTGAGCAAA 
TGGGGCCTGA ACATCTTTTG TGTGTCGGAG TACGCTGGAG GCCGCTCACT CAGCTGTATC 
ATGTATACGA TATTCCAGGA GCGGGACCTA CTGAAGAAAT TCCACATCCC TGTGGACACC 
ATGATGATGT ACATGCTGAC CCTGGAGGAC CACTACCATG CCGACGTGGC CTACCACAAC 
AGCCTGCACG CAGCGGATGT GCTGCAGTCC ACACACGTGC TGCTGGCCAC GCCCGCACTG 
GACGCTGTGT TCACAGACCT GGAGATTCTT GCTGCCCTCT TCGCTGCTGC CATCCACGAT 
GTGGACCACC CTGGCGTCTC CAACCAGTTC CTAATCAACA CCAATTCGGA GCTGGCGTTG 
ATGTACAACG ATGAGTCTGT GCTTGAGAAC CACCACCTGG CTGTGGGATT CAAGCTGCTG 
CAAGAAGAGA ACTGCGACAT CTTCCAGAAC CTCAGCAAGC GCCAGCGGCA GAGCCTGCGC 
AAGATGGTCA TCGACATGGT GCTGGCCACA GACATGTCCA AACACATGAC CCTCCTGGCT 
GACCTGAAGA CTATGGTGGA GACGAAGAAA GTGACCAGCT CCGGAGTTCT CTTGCTGGAC 
AACTACTCTG ACCGTATCCA GGTCCTCAGG AACATGGTGC ACTGTGCAGA CCTCAGCAAT 
CCCACCAAGC CCCTGGAGCT GTACCGACAG TGGACCGACC GCATCATGGC TGAGTTCTTC 
CAGCAGGGCG ACCGAGAACG GGAGCGTGGA ATGGAGATCA GCCCCATGTG CGACAAGCAC 
ACAGCCTCTG TGGAGAAGTC TCAGGTGGGC TTCATCGACT ACATTGTTCA TCCATTGTGG 
GAGACATGGG CAGATCTCGT CCACCCGGAT GCCCAAGACA TCCTGGACAC GCTGGAAGAC 
AACCGGGACT GGTACCACAG TGCCATTCGG CAGAGTCCTT CCCCACCCCT GGAAGAGGAG 
CCAGGGGGGC TTGGCCATCC GTCCCTGCCT GACAAGTTCC AATTTGAGCT CACCTTGGAG 
GAAGAGGAGG AAGAGGATTC CTTGGAGGTT CCAGGATTGC CTACCACTGA GGAAACCTTC 
CTGGCTGCAG AGGATGCCAG AGCTCAAGCT GTGGACTGGT CAAAGGTCAA AGGCCCGAGC 
ACTACAGTGG TGGAAGTGGC AGAGCGCTTG AAGCAGGAGA CCGCCTCAGC ATATGGTGCT 
CCTCAGGAGT CCATGGAGGC TGTAGGCTGT TCCTTCAGCC CTGGGACCCC TATTCTGCCT 
GACGTGAGGA CCCTATCCTC CTCAGAGGAG GCCCCGGGCC TCCTGGGCCT CCCCTCCACG 
GCGGCAGAGG TGGAGGCCCC AAGAGACCAT CTGGCTGCCA CGAGGGCTTG TTCTGCCTGC 
TCTGGGACCT CAGGAGACAA TTCTGCCATC ATCTCTGCTC CAGGCAGGTG GGGGTCAGGC 
GGAGACCCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037233.3
CDS	90..2624
Translation	MEPPAAPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYPDSAERSETERSPHRPIERADAVDTGDRPGLRTTRMSWPSSFHGTGTGGGSSRRLEAENGPTPSPGRSPLDSQASPGLVLHAGATTSQRRESFLYRSDSDYDMSPKAVSRSSSVASEAHAEDLIVTPFAQVLASLRSVRSNFSLLTNVPIPSNKRSPLGGPPSVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNEVEIPSPTPRQRAFQQPPPSVLRQSQPMSQITGLKKLVHTGSLNTNVPRFGVKTDQEDLLAQELENLSKWGLNIFCVSEYAGGRSLSCIMYTIFQERDLLKKFHIPVDTMMMYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEENCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNRDWYHSAIRQSPSPPLEEEPGGLGHPSLPDKFQFELTLEEEEEEDSLEVPGLPTTEETFLAAEDARAQAVDWSKVKGPSTTVVEVAERLKQETASAYGAPQESMEAVGCSFSPGTPILPDVRTLSSSEEAPGLLGLPSTAAEVEAPRDHLAATRACSACSGTSGDNSAIISAPGRWGSGGDPA

Target ORF information:

RefSeq Version	NM_013101.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 4A (Pde4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013101.3

ORF Insert Sequence:

ATGGAGCCTC CGGCCGCCCC CTCGGAAAGG AGCCTGTCTC TCTCTCTTCC GGGGCCCCGG 
GAGGGCCAGG CCACCCTGAA GCCGCCCCCC CAGCACCTGT GGCGGCAGCC GAGGACCCCG 
ATCCGCATCC AGCAACGCGG CTACCCGGAC AGTGCCGAGC GCTCAGAGAC AGAGCGCTCA 
CCGCACCGGC CCATAGAGCG CGCCGACGCC GTGGACACTG GCGACCGGCC AGGCCTGCGG 
ACTACCCGCA TGTCCTGGCC CTCGTCCTTC CACGGCACCG GTACCGGCGG AGGCAGCAGT 
AGGCGCTTGG AGGCAGAAAA TGGGCCAACG CCATCCCCTG GCCGCAGCCC CCTGGACTCG 
CAGGCGAGCC CGGGGCTTGT GCTGCATGCT GGGGCCACCA CCAGCCAGCG CCGCGAGTCC 
TTCCTCTACC GCTCAGACAG CGACTATGAC ATGTCACCGA AGGCTGTGTC CAGGAGCTCG 
TCTGTCGCCA GCGAAGCGCA CGCTGAGGAC CTCATTGTGA CACCATTTGC CCAGGTGCTG 
GCCAGTCTCC GCAGCGTTCG AAGCAACTTC TCACTCTTAA CCAATGTGCC CATCCCCAGC 
AACAAGAGGT CTCCACTGGG TGGCCCACCC TCTGTCTGCA AGGCCACACT GTCAGAGGAG 
ACGTGCCAGC AGCTGGCCCG GGAGACCCTG GAAGAGCTGG ATTGGTGCCT GGAGCAGCTG 
GAGACCATGC AGACCTACCG CTCTGTCAGC GAGATGGCCT CACACAAGTT CAAAAGGATG 
CTGAACCGTG AGCTCACACA CCTGTCGGAA ATGAGCAGGT CAGGAAACCA GGTCTCAGAG 
TACATTTCCA ACACATTCCT GGACAAGCAG AATGAAGTGG AGATCCCCTC ACCCACACCT 
CGGCAGAGAG CCTTCCAGCA GCCCCCGCCG TCAGTGCTGC GACAGTCCCA GCCCATGTCT 
CAGATCACAG GGCTGAAAAA GCTGGTACAC ACTGGAAGCT TGAACACCAA CGTCCCACGG 
TTTGGAGTCA AGACAGATCA AGAGGACCTC TTAGCACAAG AACTGGAGAA CTTGAGCAAA 
TGGGGCCTGA ACATCTTTTG TGTGTCGGAG TACGCTGGAG GCCGCTCACT CAGCTGTATC 
ATGTATACGA TATTCCAGGA GCGGGACCTA CTGAAGAAAT TCCACATCCC TGTGGACACC 
ATGATGATGT ACATGCTGAC CCTGGAGGAC CACTACCATG CCGACGTGGC CTACCACAAC 
AGCCTGCACG CAGCGGATGT GCTGCAGTCC ACACACGTGC TGCTGGCCAC GCCCGCACTG 
GACGCTGTGT TCACAGACCT GGAGATTCTT GCTGCCCTCT TCGCTGCTGC CATCCACGAT 
GTGGACCACC CTGGCGTCTC CAACCAGTTC CTAATCAACA CCAATTCGGA GCTGGCGTTG 
ATGTACAACG ATGAGTCTGT GCTTGAGAAC CACCACCTGG CTGTGGGATT CAAGCTGCTG 
CAAGAAGAGA ACTGCGACAT CTTCCAGAAC CTCAGCAAGC GCCAGCGGCA GAGCCTGCGC 
AAGATGGTCA TCGACATGGT GCTGGCCACA GACATGTCCA AACACATGAC CCTCCTGGCT 
GACCTGAAGA CTATGGTGGA GACGAAGAAA GTGACCAGCT CCGGAGTTCT CTTGCTGGAC 
AACTACTCTG ACCGTATCCA GGTCCTCAGG AACATGGTGC ACTGTGCAGA CCTCAGCAAT 
CCCACCAAGC CCCTGGAGCT GTACCGACAG TGGACCGACC GCATCATGGC TGAGTTCTTC 
CAGCAGGGCG ACCGAGAACG GGAGCGTGGA ATGGAGATCA GCCCCATGTG CGACAAGCAC 
ACAGCCTCTG TGGAGAAGTC TCAGGTGGGC TTCATCGACT ACATTGTTCA TCCATTGTGG 
GAGACATGGG CAGATCTCGT CCACCCGGAT GCCCAAGACA TCCTGGACAC GCTGGAAGAC 
AACCGGGACT GGTACCACAG TGCCATTCGG CAGAGTCCTT CCCCACCCCT GGAAGAGGAG 
CCAGGGGGGC TTGGCCATCC GTCCCTGCCT GACAAGTTCC AATTTGAGCT CACCTTGGAG 
GAAGAGGAGG AAGAGGATTC CTTGGAGGTT CCAGGATTGC CTACCACTGA GGAAACCTTC 
CTGGCTGCAG AGGATGCCAG AGCTCAAGCT GTGGACTGGT CAAAGGTCAA AGGCCCGAGC 
ACTACAGTGG TGGAAGTGGC AGAGCGCTTG AAGCAGGAGA CCGCCTCAGC ATATGGTGCT 
CCTCAGGAGT CCATGGAGGC TGTAGGCTGT TCCTTCAGCC CTGGGACCCC TATTCTGCCT 
GACGTGAGGA CCCTATCCTC CTCAGAGGAG GCCCCGGGCC TCCTGGGCCT CCCCTCCACG 
GCGGCAGAGG TGGAGGCCCC AAGAGACCAT CTGGCTGCCA CGAGGGCTTG TTCTGCCTGC 
TCTGGGACCT CAGGAGACAA TTCTGCCATC ATCTCTGCTC CAGGCAGGTG GGGGTCAGGC 
GGAGACCCTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Compartmentalized PDE4A5 Signaling Impairs Hippocampal Synaptic Plasticity and Long-Term Memory.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(34)8936-46(2016 08)
Havekes R,Park AJ,Tolentino RE,Bruinenberg VM,Tudor JC,Lee Y,Hansen RT,Guercio LA,Linton E,Neves-Zaph SR,Meerlo P,Baillie GS,Houslay MD,Abel T

Rolipram attenuates bile duct ligation-induced liver injury in rats: a potential pathogenic role of PDE4.
The Journal of pharmacology and experimental therapeutics347(1)80-90(2013 Oct)
Gobejishvili L,Barve S,Breitkopf-Heinlein K,Li Y,Zhang J,Avila DV,Dooley S,McClain CJ

ERK regulation of phosphodiesterase 4 enhances dopamine-stimulated AMPA receptor membrane insertion.
Proceedings of the National Academy of Sciences of the United States of America110(38)15437-42(2013 Sep)
Song RS,Massenburg B,Wenderski W,Jayaraman V,Thompson L,Neves SR

Phosphoinositide 3-kinase ? protects against catecholamine-induced ventricular arrhythmia through protein kinase A-mediated regulation of distinct phosphodiesterases.
Circulation126(17)2073-83(2012 Oct)
Ghigo A,Perino A,Mehel H,Zahradn?kov? A Jr,Morello F,Leroy J,Nikolaev VO,Damilano F,Cimino J,De Luca E,Richter W,Westenbroek R,Catterall WA,Zhang J,Yan C,Conti M,Gomez AM,Vandecasteele G,Hirsch E,Fischmeister R

?-Adrenergic cAMP signals are predominantly regulated by phosphodiesterase type 4 in cultured adult rat aortic smooth muscle cells.
PloS one7(10)e47826(2012)
Zhai K,Hubert F,Nicolas V,Ji G,Fischmeister R,Leblais V

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Pde4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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