Acadsb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acadsb
Entrez Gene ID	25618
Full Name	acyl-CoA dehydrogenase, short/branched chain
General protein information	Preferred Names acyl-CoA dehydrogenase, short/branched chain Names short/branched chain specific acyl-CoA dehydrogenase, mitochondrial 2-MEBCAD 2-methyl branched chain acyl-CoA dehydrogenase 2-methylbutyryl-CoA dehydrogenase 2-methylbutyryl-coenzyme A dehydrogenase Acyl-Coenzyme A dehydrogenase short-branched chain SBCAD acyl-Coenzyme A dehydrogenase, short/branched chain
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41
Summary	catalyzes the alpha, beta-dehydrogenation of valproyl-CoA and other acyl-CoA esters in fatty acid metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013084.2	NP_037216.1	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
XM_008759865.2	XP_008758087.1	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform X1
XM_008759867.2	XP_008758089.1	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform X3
XM_008759866.2	XP_008758088.1	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	acadsb	NP_001005724.1
Mus musculus (house mouse)	Acadsb	NP_080102.1
Drosophila melanogaster (fruit fly)	CG3902	NP_649069.2
Caenorhabditis elegans (roundworm)	acdh-3	NP_491859.1
Caenorhabditis elegans (roundworm)	acdh-1	NP_491871.1
Magnaporthe oryzae (rice blast fungus)	MGG_08690	XP_003719291.1
Neurospora crassa	NCU06543	XP_962250.1
Homo sapiens (human)	ACADSB	NP_001600.1
Canis lupus familiaris (dog)	ACADSB	XP_535048.5
Bos taurus (cattle)	ACADSB	NP_001017933.1
Rattus norvegicus (Norway rat)	Acadsb	NP_037216.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009783	XP_318878.3
Gallus gallus (chicken)	ACADSB	NP_001026411.1
Pan troglodytes (chimpanzee)	ACADSB	XP_001151318.2
	ACADSB	XP_001104844.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparative studies of Acyl-CoA dehydrogenases for monomethyl branched chain substrates in amino acid metabolism.
Liu X, Wu L, Deng G, Chen G, Li N, Chu X, Li D
Bioorganic chemistry471-8(2013 Apr)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

A novel approach to the characterization of substrate specificity in short/branched chain Acyl-CoA dehydrogenase.
He M, Burghardt TP, Vockley J
The Journal of biological chemistry278(39)37974-86(2003 Sep)

RefSeq and LocusLink: NCBI gene-centered resources.
Pruitt KD, Maglott DR
Nucleic acids research29(1)137-40(2001 Jan)

Isolated 2-methylbutyrylglycinuria caused by short/branched-chain acyl-CoA dehydrogenase deficiency: identification of a new enzyme defect, resolution of its molecular basis, and evidence for distinct acyl-CoA dehydrogenases in isoleucine and valine metabolism.
Andresen BS, Christensen E, Corydon TJ, Bross P, Pilgaard B, Wanders RJ, Ruiter JP, Simonsen H, Winter V, Knudsen I, Schroeder LD, Gregersen N, Skovby F
American journal of human genetics67(5)1095-103(2000 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Short/branched chain acyl-CoA dehydrogenase has its catalytic properties relatively similar to those of straight-chain acyl-CoA dehydrogenases in terms of their isomerase activity and enzyme inhibition.
Title: Comparative studies of Acyl-CoA dehydrogenases for monomethyl branched chain substrates in amino acid metabolism.

Differences between the rat and human enzyme at positions 383, 222, and 220 alter substrate specificity without affecting substrate binding.
Title: A novel approach to the characterization of substrate specificity in short/branched chain Acyl-CoA dehydrogenase.

The following Acadsb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acadsb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12254	NM_013084.2 Latest version!	Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa43174	XM_008759865.2 Latest version!	Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43175	XM_008759866.2 Latest version!	Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43176	XM_008759867.2 Latest version!	Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acadsb ( NM_013084.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037216.1 Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), mRNA.

Price & Availability↑

CloneID	ORa12254
Clone ID Related Accession (Same CDS sequence)	NM_013084.2
Accession Version	NM_013084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U64451.1. On Aug 30, 2012 this sequence version replaced NM_013084.1. ##Evidence-Data-START## Transcript exon combination :: U64451.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGTGT CTGCCTTCCA GCTGTGGCGT GCGGGCGGGC TGCTAAGAAG AAACTTCCTG 
ACTCACTCGT CTTCTTGGAA AATCCCTCCT CGTGTCCTCA AATCCTCACA ACCAGAAGCT 
CTACTCAGTG TAACAAATAA TGCCCTATGT TTCGCACCTC TGCAGACATT TACTGATGAG 
GATATTATGA TGCAGAAGGC AGTCAAAAAA TTTGCACAGG AACAAATTGC TCCTTTGGTT 
TCAACTATGG ATGAAAATTC AAAAATGGAG AAATCAGTGA TACAAGGATT ATTCCAACAA 
GGGATGATGG GCATTGAAGT TGAAGCAAAA TATGGAGGAA CAGAAGCCTC CTTCTTGTGC 
TCTGTCCTGG TGATAGAGGA ACTAGCTAAG GTGGATGCGT CCGTGGCTCT CCTATGTGAC 
ATCCAGAACA CAGTCATTAA CAAACTGTTT AGGAAGCACG GGACAGAAGA ACAGAAGGCC 
ACCTATTTGC CAAAGCTGGT TACGGAAAAA TTAGGGAGTT TTTGCCTCTC TGAAGCTGGA 
GCTGGTAGCG ACTCTTTTGC TTTGAAAACA AGAGCTGATA AAAGTGGAAA TTACTACGTC 
ATCAATGGAT CGAAGATGTG GATCAGCAAT GCTGAACATG CAGAGCTCTT CCTGGTTTTT 
GCAAATGTGG ACCCCCCCTC TGGGTACAGA GGCATCACCT GCTTCTTAGT AGACCGAGAT 
ACAGAAGGTT TTCAAATAGG GAGACGAGAA AACAAAATGG GGATCAGAGC TTCTTCCACC 
TGTCAGTTAA CATTTGAAAA TGTCAAGGTT CCAGAAACCA GTGTTTTGGG GAAAATTGGG 
CACGGTTATA AATATGCCAT AGGAAGTCTT AACGAAGGTA GAATTGGAAT TGCTGCCCAG 
ATGCTAGGAC TGGCCCAAGG ATGTTTTGAC TACACTATTC CATACATAAA AGAAAGGATG 
CAGTTCGGCA AACGAATATT TGATTTTCAG GGGCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCGCG GTTGCTGACG TACAACGCTG CTAGGCTTGT AGAAGCCGGG 
AGGCCATTTA TAAAAGAAGC ATCTATGGCC AAGTATTATG CATCAGAGGT CGCTGGGCTG 
ACAACAAGCA AGTGCATCGA GTGGATGGGA GGGGTCGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATTCT TCCGCGATGC GAAGATCGGT ACAATATATG AAGGAACTTC CAACATCCAG 
CTGAACACCA TTGCAAAGCA CATCGATGCA GAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037216.1
CDS	18..1316
Translation	MAVSAFQLWRAGGLLRRNFLTHSSSWKIPPRVLKSSQPEALLSVTNNALCFAPLQTFTDEDIMMQKAVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQGMMGIEVEAKYGGTEASFLCSVLVIEELAKVDASVALLCDIQNTVINKLFRKHGTEEQKATYLPKLVTEKLGSFCLSEAGAGSDSFALKTRADKSGNYYVINGSKMWISNAEHAELFLVFANVDPPSGYRGITCFLVDRDTEGFQIGRRENKMGIRASSTCQLTFENVKVPETSVLGKIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERMQFGKRIFDFQGLQHQVAHVATQLEAARLLTYNAARLVEAGRPFIKEASMAKYYASEVAGLTTSKCIEWMGGVGYTKDYPVEKFFRDAKIGTIYEGTSNIQLNTIAKHIDAEY

Target ORF information:

RefSeq Version	NM_013084.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013084.2

ORF Insert Sequence:

ATGGCGGTGT CTGCCTTCCA GCTGTGGCGT GCGGGCGGGC TGCTAAGAAG AAACTTCCTG 
ACTCACTCGT CTTCTTGGAA AATCCCTCCT CGTGTCCTCA AATCCTCACA ACCAGAAGCT 
CTACTCAGTG TAACAAATAA TGCCCTATGT TTCGCACCTC TGCAGACATT TACTGATGAG 
GATATTATGA TGCAGAAGGC AGTCAAAAAA TTTGCACAGG AACAAATTGC TCCTTTGGTT 
TCAACTATGG ATGAAAATTC AAAAATGGAG AAATCAGTGA TACAAGGATT ATTCCAACAA 
GGGATGATGG GCATTGAAGT TGAAGCAAAA TATGGAGGAA CAGAAGCCTC CTTCTTGTGC 
TCTGTCCTGG TGATAGAGGA ACTAGCTAAG GTGGATGCGT CCGTGGCTCT CCTATGTGAC 
ATCCAGAACA CAGTCATTAA CAAACTGTTT AGGAAGCACG GGACAGAAGA ACAGAAGGCC 
ACCTATTTGC CAAAGCTGGT TACGGAAAAA TTAGGGAGTT TTTGCCTCTC TGAAGCTGGA 
GCTGGTAGCG ACTCTTTTGC TTTGAAAACA AGAGCTGATA AAAGTGGAAA TTACTACGTC 
ATCAATGGAT CGAAGATGTG GATCAGCAAT GCTGAACATG CAGAGCTCTT CCTGGTTTTT 
GCAAATGTGG ACCCCCCCTC TGGGTACAGA GGCATCACCT GCTTCTTAGT AGACCGAGAT 
ACAGAAGGTT TTCAAATAGG GAGACGAGAA AACAAAATGG GGATCAGAGC TTCTTCCACC 
TGTCAGTTAA CATTTGAAAA TGTCAAGGTT CCAGAAACCA GTGTTTTGGG GAAAATTGGG 
CACGGTTATA AATATGCCAT AGGAAGTCTT AACGAAGGTA GAATTGGAAT TGCTGCCCAG 
ATGCTAGGAC TGGCCCAAGG ATGTTTTGAC TACACTATTC CATACATAAA AGAAAGGATG 
CAGTTCGGCA AACGAATATT TGATTTTCAG GGGCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCGCG GTTGCTGACG TACAACGCTG CTAGGCTTGT AGAAGCCGGG 
AGGCCATTTA TAAAAGAAGC ATCTATGGCC AAGTATTATG CATCAGAGGT CGCTGGGCTG 
ACAACAAGCA AGTGCATCGA GTGGATGGGA GGGGTCGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATTCT TCCGCGATGC GAAGATCGGT ACAATATATG AAGGAACTTC CAACATCCAG 
CTGAACACCA TTGCAAAGCA CATCGATGCA GAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acadsb ( XM_008759865.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758087.1 Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43174
Clone ID Related Accession (Same CDS sequence)	XM_008759865.2
Accession Version	XM_008759865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CTGCCTTCCA GCTGTGGCGT GCGGGCGGGC TGCTAAGAAG AAACTTCCTG 
ACTCACTCGT CTTCTTGGAA AATCCCTCCT CGTGTCCTCA AATCCTCACA ACCAGAAGCT 
CTACTCAGTG TAACAAATAA TGCCCTATGT TTCGCACCTC TGCAGACATT TACTGATGAG 
GATATTATGA TGCAGAAGGC AGTCAAAAAA TTTGCACAGG AACAAATTGC TCCTTTGGTT 
TCAACTATGG ATGAAAATTC AAAAATGGAG AAATCAGTGA TACAAGGATT ATTCCAACAA 
GGGATGATGG GCATTGAAGT TGAAGCAAAA TATGGAGGAA CAGAAGCCTC CTTCTTGTGC 
TCTGTCCTGG TGATAGAGGA ACTAGCTAAG GTGGATGCGT CCGTGGCTCT CCTATGTGAC 
ATCCAGAACA CAGTCATTAA CAAACTGTTT AGGAAGCACG GGACAGAAGA ACAGAAGGCC 
ACCTATTTGC CAAAGCTGGT TACGGAAAAA TTAGGGAGTT TTTGCCTCTC TGAAGCTGGA 
GCTGGTAGCG ACTCTTTTGC TTTGAAAACA AGAGCTGATA AAAGTGGAAA TTACTACGTC 
ATCAATGGAT CGAAGATGTG GATCAGCAAT GCTGAACATG CAGAGCTCTT CCTGGTTTTT 
GCAAATGTGG ACCCCCCCTC TGGGTACAGA GGCATCACCT GCTTCTTAGT AGACCGAGAT 
ACAGAAGGTT TTCAAATAGG GAGACGAGAA AACAAAATGG GGATCAGAGC TTCTTCCACC 
TGTCAGTTAA CATTTGAAAA TGTCAAGGTT CCAGAAACCA GTGTTTTGGG GAAAATTGGG 
CACGGTTATA AATATGCCAT AGGAAGTCTT AACGAAGGTA GAATTGGAAT TGCTGCCCAG 
ATGCTAGGAC TGGCCCAAGG ATGTTTTGAC TACACTATTC CATACATAAA AGAAAGGATG 
CAGTTCGGCA AACGAATATT TGATTTTCAG GGGCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCGCG GTTGCTGACG TACAACGCTG CTAGGCTTGT AGAAGCCGGG 
AGGCCATTTA TAAAAGAAGC ATCTATGGCC AAGTATTATG CATCAGAGGT CGCTGGGCTG 
ACAACAAGCA AGTGCATCGA GTGGATGGGA GGGGTCGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATTCT TCCGCGATGC GAAGATCGGT GAGCAGACTT TCTACGTTCT CTTGTTTTAT 
TTGCCACTGT GGGTATTTGG GCTATTGTGT TTGTCGATTT TATATTACTG GTACTTAGGT 
AAAAGCATTT TAATCTCTAC AGAATGTTTA TTGACTTTTC TTTGTGGCTG GATTTCTTCC 
TTCTTCCTAT CAGAAAAAAA TGAATTTATA TTTCTAAAAA CATCAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758087.1
CDS	108..1538
Translation	MAVSAFQLWRAGGLLRRNFLTHSSSWKIPPRVLKSSQPEALLSVTNNALCFAPLQTFTDEDIMMQKAVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQGMMGIEVEAKYGGTEASFLCSVLVIEELAKVDASVALLCDIQNTVINKLFRKHGTEEQKATYLPKLVTEKLGSFCLSEAGAGSDSFALKTRADKSGNYYVINGSKMWISNAEHAELFLVFANVDPPSGYRGITCFLVDRDTEGFQIGRRENKMGIRASSTCQLTFENVKVPETSVLGKIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERMQFGKRIFDFQGLQHQVAHVATQLEAARLLTYNAARLVEAGRPFIKEASMAKYYASEVAGLTTSKCIEWMGGVGYTKDYPVEKFFRDAKIGEQTFYVLLFYLPLWVFGLLCLSILYYWYLGKSILISTECLLTFLCGWISSFFLSEKNEFIFLKTSN

Target ORF information:

RefSeq Version	XM_008759865.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759865.2

ORF Insert Sequence:

ATGGCGGTGT CTGCCTTCCA GCTGTGGCGT GCGGGCGGGC TGCTAAGAAG AAACTTCCTG 
ACTCACTCGT CTTCTTGGAA AATCCCTCCT CGTGTCCTCA AATCCTCACA ACCAGAAGCT 
CTACTCAGTG TAACAAATAA TGCCCTATGT TTCGCACCTC TGCAGACATT TACTGATGAG 
GATATTATGA TGCAGAAGGC AGTCAAAAAA TTTGCACAGG AACAAATTGC TCCTTTGGTT 
TCAACTATGG ATGAAAATTC AAAAATGGAG AAATCAGTGA TACAAGGATT ATTCCAACAA 
GGGATGATGG GCATTGAAGT TGAAGCAAAA TATGGAGGAA CAGAAGCCTC CTTCTTGTGC 
TCTGTCCTGG TGATAGAGGA ACTAGCTAAG GTGGATGCGT CCGTGGCTCT CCTATGTGAC 
ATCCAGAACA CAGTCATTAA CAAACTGTTT AGGAAGCACG GGACAGAAGA ACAGAAGGCC 
ACCTATTTGC CAAAGCTGGT TACGGAAAAA TTAGGGAGTT TTTGCCTCTC TGAAGCTGGA 
GCTGGTAGCG ACTCTTTTGC TTTGAAAACA AGAGCTGATA AAAGTGGAAA TTACTACGTC 
ATCAATGGAT CGAAGATGTG GATCAGCAAT GCTGAACATG CAGAGCTCTT CCTGGTTTTT 
GCAAATGTGG ACCCCCCCTC TGGGTACAGA GGCATCACCT GCTTCTTAGT AGACCGAGAT 
ACAGAAGGTT TTCAAATAGG GAGACGAGAA AACAAAATGG GGATCAGAGC TTCTTCCACC 
TGTCAGTTAA CATTTGAAAA TGTCAAGGTT CCAGAAACCA GTGTTTTGGG GAAAATTGGG 
CACGGTTATA AATATGCCAT AGGAAGTCTT AACGAAGGTA GAATTGGAAT TGCTGCCCAG 
ATGCTAGGAC TGGCCCAAGG ATGTTTTGAC TACACTATTC CATACATAAA AGAAAGGATG 
CAGTTCGGCA AACGAATATT TGATTTTCAG GGGCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCGCG GTTGCTGACG TACAACGCTG CTAGGCTTGT AGAAGCCGGG 
AGGCCATTTA TAAAAGAAGC ATCTATGGCC AAGTATTATG CATCAGAGGT CGCTGGGCTG 
ACAACAAGCA AGTGCATCGA GTGGATGGGA GGGGTCGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATTCT TCCGCGATGC GAAGATCGGT GAGCAGACTT TCTACGTTCT CTTGTTTTAT 
TTGCCACTGT GGGTATTTGG GCTATTGTGT TTGTCGATTT TATATTACTG GTACTTAGGT 
AAAAGCATTT TAATCTCTAC AGAATGTTTA TTGACTTTTC TTTGTGGCTG GATTTCTTCC 
TTCTTCCTAT CAGAAAAAAA TGAATTTATA TTTCTAAAAA CATCAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acadsb ( XM_008759866.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758088.1 Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43175
Clone ID Related Accession (Same CDS sequence)	XM_008759866.2
Accession Version	XM_008759866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CTGCCTTCCA GCTGTGGCGT GCGGGCGGGC TGCTAAGAAG AAACTTCCTG 
ACTCACTCGT CTTCTTGGAA AATCCCTCCT CGTGTCCTCA AATCCTCACA ACCAGAAGCT 
CTACTCAGTG TAACAAATAA TGCCCTATGT TTCGCACCTC TGCAGACATT TACTGATGAG 
GATATTATGA TGCAGAAGGC AGTCAAAAAA TTTGCACAGG AACAAATTGC TCCTTTGGTT 
TCAACTATGG ATGAAAATTC AAAAATGGAG AAATCAGTGA TACAAGGATT ATTCCAACAA 
GGGATGATGG GCATTGAAGT TGAAGCAAAA TATGGAGGAA CAGAAGCCTC CTTCTTGTGC 
TCTGTCCTGG TGATAGAGGA ACTAGCTAAG GTGGATGCGT CCGTGGCTCT CCTATGTGAC 
ATCCAGAACA CAGTCATTAA CAAACTGTTT AGGAAGCACG GGACAGAAGA ACAGAAGGCC 
ACCTATTTGC CAAAGCTGGT TACGGAAAAA TTAGGGAGTT TTTGCCTCTC TGAAGCTGGA 
GCTGGTAGCG ACTCTTTTGC TTTGAAAACA AGAGCTGATA AAAGTGGAAA TTACTACGTC 
ATCAATGGAT CGAAGATGTG GATCAGCAAT GCTGAACATG CAGAGCTCTT CCTGGTTTTT 
GCAAATGTGG ACCCCCCCTC TGGGTACAGA GGCATCACCT GCTTCTTAGT AGACCGAGAT 
ACAGAAGGTT TTCAAATAGG GAGACGAGAA AACAAAATGG GGATCAGAGC TTCTTCCACC 
TGTCAGTTAA CATTTGAAAA TGTCAAGGTT CCAGAAACCA GTGTTTTGGG GAAAATTGGG 
CACGGTTATA AATATGCCAT AGGAAGTCTT AACGAAGGTA GAATTGGAAT TGCTGCCCAG 
ATGCTAGGAC TGGCCCAAGG ATGTTTTGAC TACACTATTC CATACATAAA AGAAAGGATG 
CAGTTCGGCA AACGAATATT TGATTTTCAG GGGCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCGCG GTTGCTGACG TACAACGCTG CTAGGCTTGT AGAAGCCGGG 
AGGCCATTTA TAAAAGAAGC ATCTATGGCC AAGTATTATG CATCAGAGGT CGCTGGGCTG 
ACAACAAGCA AGTGCATCGA GTGGATGGGA GGGGTCGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATTCT TCCGCGATGC GAAGATCGAA GGCAGCTGCC TAATCACGCC TGAGTGCTCC 
AGTCCTCCAC CAGCAGCGTG GAGAGCGAAC GGCGGTCCCG AAAGCTGCCA GGTCCTTAGA 
AGCAGCTGGG CTTGCTCACT GATGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758088.1
CDS	108..1457
Translation	MAVSAFQLWRAGGLLRRNFLTHSSSWKIPPRVLKSSQPEALLSVTNNALCFAPLQTFTDEDIMMQKAVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQGMMGIEVEAKYGGTEASFLCSVLVIEELAKVDASVALLCDIQNTVINKLFRKHGTEEQKATYLPKLVTEKLGSFCLSEAGAGSDSFALKTRADKSGNYYVINGSKMWISNAEHAELFLVFANVDPPSGYRGITCFLVDRDTEGFQIGRRENKMGIRASSTCQLTFENVKVPETSVLGKIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERMQFGKRIFDFQGLQHQVAHVATQLEAARLLTYNAARLVEAGRPFIKEASMAKYYASEVAGLTTSKCIEWMGGVGYTKDYPVEKFFRDAKIEGSCLITPECSSPPPAAWRANGGPESCQVLRSSWACSLML

Target ORF information:

RefSeq Version	XM_008759866.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759866.2

ORF Insert Sequence:

ATGGCGGTGT CTGCCTTCCA GCTGTGGCGT GCGGGCGGGC TGCTAAGAAG AAACTTCCTG 
ACTCACTCGT CTTCTTGGAA AATCCCTCCT CGTGTCCTCA AATCCTCACA ACCAGAAGCT 
CTACTCAGTG TAACAAATAA TGCCCTATGT TTCGCACCTC TGCAGACATT TACTGATGAG 
GATATTATGA TGCAGAAGGC AGTCAAAAAA TTTGCACAGG AACAAATTGC TCCTTTGGTT 
TCAACTATGG ATGAAAATTC AAAAATGGAG AAATCAGTGA TACAAGGATT ATTCCAACAA 
GGGATGATGG GCATTGAAGT TGAAGCAAAA TATGGAGGAA CAGAAGCCTC CTTCTTGTGC 
TCTGTCCTGG TGATAGAGGA ACTAGCTAAG GTGGATGCGT CCGTGGCTCT CCTATGTGAC 
ATCCAGAACA CAGTCATTAA CAAACTGTTT AGGAAGCACG GGACAGAAGA ACAGAAGGCC 
ACCTATTTGC CAAAGCTGGT TACGGAAAAA TTAGGGAGTT TTTGCCTCTC TGAAGCTGGA 
GCTGGTAGCG ACTCTTTTGC TTTGAAAACA AGAGCTGATA AAAGTGGAAA TTACTACGTC 
ATCAATGGAT CGAAGATGTG GATCAGCAAT GCTGAACATG CAGAGCTCTT CCTGGTTTTT 
GCAAATGTGG ACCCCCCCTC TGGGTACAGA GGCATCACCT GCTTCTTAGT AGACCGAGAT 
ACAGAAGGTT TTCAAATAGG GAGACGAGAA AACAAAATGG GGATCAGAGC TTCTTCCACC 
TGTCAGTTAA CATTTGAAAA TGTCAAGGTT CCAGAAACCA GTGTTTTGGG GAAAATTGGG 
CACGGTTATA AATATGCCAT AGGAAGTCTT AACGAAGGTA GAATTGGAAT TGCTGCCCAG 
ATGCTAGGAC TGGCCCAAGG ATGTTTTGAC TACACTATTC CATACATAAA AGAAAGGATG 
CAGTTCGGCA AACGAATATT TGATTTTCAG GGGCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCGCG GTTGCTGACG TACAACGCTG CTAGGCTTGT AGAAGCCGGG 
AGGCCATTTA TAAAAGAAGC ATCTATGGCC AAGTATTATG CATCAGAGGT CGCTGGGCTG 
ACAACAAGCA AGTGCATCGA GTGGATGGGA GGGGTCGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATTCT TCCGCGATGC GAAGATCGAA GGCAGCTGCC TAATCACGCC TGAGTGCTCC 
AGTCCTCCAC CAGCAGCGTG GAGAGCGAAC GGCGGTCCCG AAAGCTGCCA GGTCCTTAGA 
AGCAGCTGGG CTTGCTCACT GATGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acadsb ( XM_008759867.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758089.1 Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X3, mRNA.

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CloneID	ORa43176
Clone ID Related Accession (Same CDS sequence)	XM_008759867.2
Accession Version	XM_008759867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGT CTGCCTTCCA GCTGTGGCGT GCGGGCGGGC TGCTAAGAAG AAACTTCCTG 
ACTCACTCGT CTTCTTGGAA AATCCCTCCT CGTGTCCTCA AATCCTCACA ACCAGAAGCT 
CTACTCAGTG TAACAAATAA TGCCCTATGT TTCGCACCTC TGCAGACATT TACTGATGAG 
GATATTATGA TGCAGAAGGC AGTCAAAAAA TTTGCACAGG AACAAATTGC TCCTTTGGTT 
TCAACTATGG ATGAAAATTC AAAAATGGAG AAATCAGTGA TACAAGGATT ATTCCAACAA 
GGGATGATGG GCATTGAAGT TGAAGCAAAA TATGGAGGAA CAGAAGCCTC CTTCTTGTGC 
TCTGTCCTGG TGATAGAGGA ACTAGCTAAG GTGGATGCGT CCGTGGCTCT CCTATGTGAC 
ATCCAGAACA CAGTCATTAA CAAACTGTTT AGGAAGCACG GGACAGAAGA ACAGAAGGCC 
ACCTATTTGC CAAAGCTGGT TACGGAAAAA TTAGGGAGTT TTTGCCTCTC TGAAGCTGGA 
GCTGGTAGCG ACTCTTTTGC TTTGAAAACA AGAGCTGATA AAAGTGGAAA TTACTACGTC 
ATCAATGGAT CGAAGATGTG GATCAGCAAT GCTGAACATG CAGAGCTCTT CCTGGTTTTT 
GCAAATGTGG ACCCCCCCTC TGGGTACAGA GGCATCACCT GCTTCTTAGT AGACCGAGAT 
ACAGAAGGTT TTCAAATAGG GAGACGAGAA AACAAAATGG GGATCAGAGC TTCTTCCACC 
TGTCAGTTAA CATTTGAAAA TGTCAAGGTT CCAGAAACCA GTGTTTTGGG GAAAATTGGG 
CACGGTTATA AATATGCCAT AGGAAGTCTT AACGAAGGTA GAATTGGAAT TGCTGCCCAG 
GGGCTCCAAC ACCAAGTGGC TCACGTGGCC ACCCAGCTGG AAGCTGCGCG GTTGCTGACG 
TACAACGCTG CTAGGCTTGT AGAAGCCGGG AGGCCATTTA TAAAAGAAGC ATCTATGGCC 
AAGTATTATG CATCAGAGGT CGCTGGGCTG ACAACAAGCA AGTGCATCGA GTGGATGGGA 
GGGGTCGGCT ACACCAAAGA TTACCCTGTG GAGAAATTCT TCCGCGATGC GAAGATCGGT 
GAGCAGACTT TCTACGTTCT CTTGTTTTAT TTGCCACTGT GGGTATTTGG GCTATTGTGT 
TTGTCGATTT TATATTACTG GTACTTAGGT AAAAGCATTT TAATCTCTAC AGAATGTTTA 
TTGACTTTTC TTTGTGGCTG GATTTCTTCC TTCTTCCTAT CAGAAAAAAA TGAATTTATA 
TTTCTAAAAA CATCAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758089.1
CDS	109..1449
Translation	MAVSAFQLWRAGGLLRRNFLTHSSSWKIPPRVLKSSQPEALLSVTNNALCFAPLQTFTDEDIMMQKAVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQGMMGIEVEAKYGGTEASFLCSVLVIEELAKVDASVALLCDIQNTVINKLFRKHGTEEQKATYLPKLVTEKLGSFCLSEAGAGSDSFALKTRADKSGNYYVINGSKMWISNAEHAELFLVFANVDPPSGYRGITCFLVDRDTEGFQIGRRENKMGIRASSTCQLTFENVKVPETSVLGKIGHGYKYAIGSLNEGRIGIAAQGLQHQVAHVATQLEAARLLTYNAARLVEAGRPFIKEASMAKYYASEVAGLTTSKCIEWMGGVGYTKDYPVEKFFRDAKIGEQTFYVLLFYLPLWVFGLLCLSILYYWYLGKSILISTECLLTFLCGWISSFFLSEKNEFIFLKTSN

Target ORF information:

RefSeq Version	XM_008759867.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA dehydrogenase, short/branched chain (Acadsb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759867.2

ORF Insert Sequence:

ATGGCGGTGT CTGCCTTCCA GCTGTGGCGT GCGGGCGGGC TGCTAAGAAG AAACTTCCTG 
ACTCACTCGT CTTCTTGGAA AATCCCTCCT CGTGTCCTCA AATCCTCACA ACCAGAAGCT 
CTACTCAGTG TAACAAATAA TGCCCTATGT TTCGCACCTC TGCAGACATT TACTGATGAG 
GATATTATGA TGCAGAAGGC AGTCAAAAAA TTTGCACAGG AACAAATTGC TCCTTTGGTT 
TCAACTATGG ATGAAAATTC AAAAATGGAG AAATCAGTGA TACAAGGATT ATTCCAACAA 
GGGATGATGG GCATTGAAGT TGAAGCAAAA TATGGAGGAA CAGAAGCCTC CTTCTTGTGC 
TCTGTCCTGG TGATAGAGGA ACTAGCTAAG GTGGATGCGT CCGTGGCTCT CCTATGTGAC 
ATCCAGAACA CAGTCATTAA CAAACTGTTT AGGAAGCACG GGACAGAAGA ACAGAAGGCC 
ACCTATTTGC CAAAGCTGGT TACGGAAAAA TTAGGGAGTT TTTGCCTCTC TGAAGCTGGA 
GCTGGTAGCG ACTCTTTTGC TTTGAAAACA AGAGCTGATA AAAGTGGAAA TTACTACGTC 
ATCAATGGAT CGAAGATGTG GATCAGCAAT GCTGAACATG CAGAGCTCTT CCTGGTTTTT 
GCAAATGTGG ACCCCCCCTC TGGGTACAGA GGCATCACCT GCTTCTTAGT AGACCGAGAT 
ACAGAAGGTT TTCAAATAGG GAGACGAGAA AACAAAATGG GGATCAGAGC TTCTTCCACC 
TGTCAGTTAA CATTTGAAAA TGTCAAGGTT CCAGAAACCA GTGTTTTGGG GAAAATTGGG 
CACGGTTATA AATATGCCAT AGGAAGTCTT AACGAAGGTA GAATTGGAAT TGCTGCCCAG 
GGGCTCCAAC ACCAAGTGGC TCACGTGGCC ACCCAGCTGG AAGCTGCGCG GTTGCTGACG 
TACAACGCTG CTAGGCTTGT AGAAGCCGGG AGGCCATTTA TAAAAGAAGC ATCTATGGCC 
AAGTATTATG CATCAGAGGT CGCTGGGCTG ACAACAAGCA AGTGCATCGA GTGGATGGGA 
GGGGTCGGCT ACACCAAAGA TTACCCTGTG GAGAAATTCT TCCGCGATGC GAAGATCGGT 
GAGCAGACTT TCTACGTTCT CTTGTTTTAT TTGCCACTGT GGGTATTTGG GCTATTGTGT 
TTGTCGATTT TATATTACTG GTACTTAGGT AAAAGCATTT TAATCTCTAC AGAATGTTTA 
TTGACTTTTC TTTGTGGCTG GATTTCTTCC TTCTTCCTAT CAGAAAAAAA TGAATTTATA 
TTTCTAAAAA CATCAAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative studies of Acyl-CoA dehydrogenases for monomethyl branched chain substrates in amino acid metabolism.
Bioorganic chemistry471-8(2013 Apr)
Liu X,Wu L,Deng G,Chen G,Li N,Chu X,Li D

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

A novel approach to the characterization of substrate specificity in short/branched chain Acyl-CoA dehydrogenase.
The Journal of biological chemistry278(39)37974-86(2003 Sep)
He M,Burghardt TP,Vockley J

RefSeq and LocusLink: NCBI gene-centered resources.
Nucleic acids research29(1)137-40(2001 Jan)
Pruitt KD,Maglott DR

Isolated 2-methylbutyrylglycinuria caused by short/branched-chain acyl-CoA dehydrogenase deficiency: identification of a new enzyme defect, resolution of its molecular basis, and evidence for distinct acyl-CoA dehydrogenases in isoleucine and valine metabolism.
American journal of human genetics67(5)1095-103(2000 Nov)
Andresen BS,Christensen E,Corydon TJ,Bross P,Pilgaard B,Wanders RJ,Ruiter JP,Simonsen H,Winter V,Knudsen I,Schroeder LD,Gregersen N,Skovby F

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Acadsb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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