Chrna4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrna4
Entrez Gene ID	25590
Full Name	cholinergic receptor nicotinic alpha 4 subunit
Synonyms	NARAC
General protein information	Preferred Names cholinergic receptor nicotinic alpha 4 subunit Names neuronal acetylcholine receptor subunit alpha-4 Neuronal nicotinic acetylcholine receptor alpha 4 subunit cholinergic receptor, nicotinic, alpha 4 (neuronal) cholinergic receptor, nicotinic, alpha polypeptide 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q43
Summary	nicotinic acetylcholine receptor subunit responsible for binding calcium sensor protein visinin-like protein-1 (Vilip1), which modulates acetylcholine receptor expression and sensitivity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024354.1	NP_077330.1	neuronal acetylcholine receptor subunit alpha-4 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRNA4	XP_543097.3
Danio rerio (zebrafish)	chrna4b	NP_001041528.1
Xenopus tropicalis (tropical clawed frog)	chrna4	NP_001107315.1
Bos taurus (cattle)	CHRNA4	NP_001179829.1
Danio rerio (zebrafish)	LOC563696	XP_692148.2
Mus musculus (house mouse)	Chrna4	NP_056545.3
Rattus norvegicus (Norway rat)	Chrna4	NP_077330.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000966	XP_309158.3
	CHRNA4	XP_001114265.2
Gallus gallus (chicken)	CHRNA4	NP_990145.1
Homo sapiens (human)	CHRNA4	NP_000735.1
Pan troglodytes (chimpanzee)	CHRNA4	NP_001029286.1
Drosophila melanogaster (fruit fly)	nAcRbeta-64B	NP_523927.2

Gene Ontology
Bibliography

Related articles in PubMed

Acute Ethanol Administration Upregulates Synaptic ?4-Subunit of Neuronal Nicotinic Acetylcholine Receptors within the Nucleus Accumbens and Amygdala.
Tarren JR, Lester HA, Belmer A, Bartlett SE
Frontiers in molecular neuroscience10338(2017)

Molecular simulation study of the unbinding of ?-conotoxin [?4E]GID at the ?7 and ?4?2 neuronal nicotinic acetylcholine receptors.
Suresh A, Hung A
Journal of molecular graphics & modelling70109-121(2016 Nov)

Inhibited Expression of ?
Xiao Y, Zhao L, Kuang SX, Guan ZZ
Cellular and molecular neurobiology36(8)1377-1387(2016 Nov)

Mechanisms of inhibition and potentiation of ?4?2 nicotinic acetylcholine receptors by members of the Ly6 protein family.
Wu M, Puddifoot CA, Taylor P, Joiner WJ
The Journal of biological chemistry290(40)24509-18(2015 Oct)

The modulation of nicotinic acetylcholine receptors on the neuronal network oscillations in rat hippocampal CA3 area.
Wang Y, Wang Z, Wang J, Wang Y, Henderson Z, Wang X, Zhang X, Song J, Lu C
Scientific reports59493(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings indicate that alterations in expression of the alpha4beta2 subtype of nAChR may be involved in the molecular mechanism(s) underlying the cognitive deficit associated with vascular dementia
Title: Inhibited Expression of ?

These results demonstrate that nicotine modulates gamma oscillations via a7 and a4b2 nAChR as well as NMDA activation, suggesting that nAChR activation may have a therapeutic role for the clinical disorder such as schizophrenia
Title: The modulation of nicotinic acetylcholine receptors on the neuronal network oscillations in rat hippocampal CA3 area.

maternal stress alters the development of hippocampal alpha4 beta2 (alpha4beta2 *) and alpha7 (alpha7 *) nicotinic receptor levels in adult offspring.
Title: The effects of prenatal stress on alpha4 beta2 and alpha7 hippocampal nicotinic acetylcholine receptor levels in adult offspring.

Data suggest that alpha4 beta2 nicotinic receptors (Chrna4; Chrnb2) are involved in tolerance to DOPA exhibited as drug-induced dyskinesias in late stages of Parkinson's disease.
Title: ?4?2 Nicotinic receptors play a role in the nAChR-mediated decline in L-dopa-induced dyskinesias in parkinsonian rats.

Data indicate that compounds showed favorable subtype selectivity for beta2*-nAChRs over beta4*-nAChRs.
Title: Chemistry, pharmacology, and behavioral studies identify chiral cyclopropanes as selective ?4?2-nicotinic acetylcholine receptor partial agonists exhibiting an antidepressant profile. Part II.

The following Chrna4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrna4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13513	NM_024354.1 Latest version!	Rattus norvegicus cholinergic receptor nicotinic alpha 4 subunit (Chrna4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrna4 ( NM_024354.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077330.1 Rattus norvegicus cholinergic receptor nicotinic alpha 4 subunit (Chrna4), mRNA.

Price & Availability↑

CloneID	ORa13513
Clone ID Related Accession (Same CDS sequence)	NM_024354.1
Accession Version	NM_024354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	neuronal acetylcholine receptor subunit alpha-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF007212.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF007212.1, M15682.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAATT CGGGCCCCGG GGCGCCGCCG CCGCTGCTGC TACTGCCGCT GCTGCTGCTC 
CTAGGGACCG GCCTCTTGCC TGCTAGCAGC CACATAGAGA CCCGGGCCCA TGCGGAGGAG 
CGGCTCCTGA AGAGACTCTT CTCCGGTTAC AACAAGTGGT CTCGGCCAGT AGGCAATATC 
TCAGATGTGG TCCTCGTCCG CTTTGGCTTG TCCATTGCTC AGCTCATTGA CGTGGACGAG 
AAGAACCAGA TGATGACAAC CAACGTGTGG GTGAAGCAGG AGTGGCACGA CTACAAGCTG 
CGCTGGGACC CTGGTGACTA CGAGAATGTC ACCTCCATCC GCATCCCCTC TGAACTCATC 
TGGAGGCCTG ACATCGTCCT CTACAACAAT GCGGATGGAG ACTTTGCAGT CACCCACCTG 
ACCAAGGCCC ACCTGTTCTA TGACGGAAGG GTGCAGTGGA CACCCCCAGC CATCTATAAG 
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTTGACC AGCAGAACTG TACCATGAAG 
TTTGGATCCT GGACCTACGA CAAGGCCAAG ATTGACTTAG TGAGCATTCA TAGCCGTGTG 
GACCAACTGG ACTTCTGGGA AAGTGGGGAG TGGGTCATCG TGGATGCTGT GGGCACCTAC 
AACACCAGGA AGTACGAGTG CTGTGCCGAG ATCTATCCTG ACATCACCTA TGCCTTCATC 
ATCCGACGCC TGCCGCTATT CTACACCATC AACCTCATCA TCCCGTGCCT GCTCATCTCC 
TGTCTCACCG TGCTGGTCTT CTATCTGCCT TCAGAGTGTG GCGAGAAGGT CACACTGTGC 
ATCTCGGTGC TGCTTTCTCT CACCGTCTTC CTGCTGCTCA TCACCGAGAT CATCCCGTCC 
ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTCCTCT TCACCATGAT CTTCGTCACC 
CTCTCCATCG TCATCACGGT CTTCGTGCTC AATGTGCACC ACCGCTCGCC ACGCACACAC 
ACGATGCCCG CCTGGGTGCG TAGAGTCTTC CTGGACATCG TGCCTCGCCT CCTCTTCATG 
AAGCGCCCCT CTGTGGTCAA AGACAACTGC CGGAGACTTA TTGAGTCCAT GCACAAGATG 
GCCAACGCCC CCCGCTTCTG GCCAGAGCCT GTGGGCGAGC CCGGCATCTT GAGTGACATC 
TGCAACCAAG GTCTGTCACC TGCCCCAACT TTCTGCAACC CCACGGACAC AGCAGTCGAG 
ACCCAGCCTA CGTGCAGGTC ACCCCCCCTT GAGGTCCCTG ACTTGAAGAC ATCAGAGGTT 
GAGAAGGCCA GTCCCTGTCC ATCGCCTGGC TCCTGTCCTC CACCCAAGAG CAGCAGTGGG 
GCTCCAATGC TCATCAAAGC CAGGTCCCTG AGTGTCCAGC ATGTGCCCAG CTCCCAAGAA 
GCAGCAGAAG ATGGCATCCG CTGCCGGTCT CGGAGTATCC AGTACTGTGT TTCCCAAGAT 
GGAGCTGCCT CCCTGGCTGA CAGCAAGCCC ACCAGCTCCC CGACCTCCCT GAAGGCCCGT 
CCATCCCAGC TTCCCGTGTC AGACCAGGCC TCTCCATGCA AATGCACATG CAAGGAACCA 
TCTCCTGTGT CCCCAGTCAC TGTGCTCAAG GCGGGAGGCA CCAAAGCACC TCCCCAACAC 
CTGCCCCTGT CACCAGCCCT GACACGGGCA GTAGAAGGCG TCCAGTACAT TGCAGACCAC 
CTCAAGGCAG AAGACACTGA CTTCTCGGTG AAGGAGGACT GGAAATACGT GGCCATGGTC 
ATTGACCGAA TCTTCCTCTG GATGTTCATC ATTGTCTGCC TTCTGGGCAC TGTGGGACTC 
TTCCTGCCTC CCTGGCTGGC TGGTATGATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077330.1
CDS	10..1902
Translation	MANSGPGAPPPLLLLPLLLLLGTGLLPASSHIETRAHAEERLLKRLFSGYNKWSRPVGNISDVVLVRFGLSIAQLIDVDEKNQMMTTNVWVKQEWHDYKLRWDPGDYENVTSIRIPSELIWRPDIVLYNNADGDFAVTHLTKAHLFYDGRVQWTPPAIYKSSCSIDVTFFPFDQQNCTMKFGSWTYDKAKIDLVSIHSRVDQLDFWESGEWVIVDAVGTYNTRKYECCAEIYPDITYAFIIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKVTLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPRTHTMPAWVRRVFLDIVPRLLFMKRPSVVKDNCRRLIESMHKMANAPRFWPEPVGEPGILSDICNQGLSPAPTFCNPTDTAVETQPTCRSPPLEVPDLKTSEVEKASPCPSPGSCPPPKSSSGAPMLIKARSLSVQHVPSSQEAAEDGIRCRSRSIQYCVSQDGAASLADSKPTSSPTSLKARPSQLPVSDQASPCKCTCKEPSPVSPVTVLKAGGTKAPPQHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDRIFLWMFIIVCLLGTVGLFLPPWLAGMI

Target ORF information:

RefSeq Version	NM_024354.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor nicotinic alpha 4 subunit (Chrna4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024354.1

ORF Insert Sequence:

ATGGCCAATT CGGGCCCCGG GGCGCCGCCG CCGCTGCTGC TACTGCCGCT GCTGCTGCTC 
CTAGGGACCG GCCTCTTGCC TGCTAGCAGC CACATAGAGA CCCGGGCCCA TGCGGAGGAG 
CGGCTCCTGA AGAGACTCTT CTCCGGTTAC AACAAGTGGT CTCGGCCAGT AGGCAATATC 
TCAGATGTGG TCCTCGTCCG CTTTGGCTTG TCCATTGCTC AGCTCATTGA CGTGGACGAG 
AAGAACCAGA TGATGACAAC CAACGTGTGG GTGAAGCAGG AGTGGCACGA CTACAAGCTG 
CGCTGGGACC CTGGTGACTA CGAGAATGTC ACCTCCATCC GCATCCCCTC TGAACTCATC 
TGGAGGCCTG ACATCGTCCT CTACAACAAT GCGGATGGAG ACTTTGCAGT CACCCACCTG 
ACCAAGGCCC ACCTGTTCTA TGACGGAAGG GTGCAGTGGA CACCCCCAGC CATCTATAAG 
AGCTCCTGCA GCATCGACGT CACCTTCTTC CCCTTTGACC AGCAGAACTG TACCATGAAG 
TTTGGATCCT GGACCTACGA CAAGGCCAAG ATTGACTTAG TGAGCATTCA TAGCCGTGTG 
GACCAACTGG ACTTCTGGGA AAGTGGGGAG TGGGTCATCG TGGATGCTGT GGGCACCTAC 
AACACCAGGA AGTACGAGTG CTGTGCCGAG ATCTATCCTG ACATCACCTA TGCCTTCATC 
ATCCGACGCC TGCCGCTATT CTACACCATC AACCTCATCA TCCCGTGCCT GCTCATCTCC 
TGTCTCACCG TGCTGGTCTT CTATCTGCCT TCAGAGTGTG GCGAGAAGGT CACACTGTGC 
ATCTCGGTGC TGCTTTCTCT CACCGTCTTC CTGCTGCTCA TCACCGAGAT CATCCCGTCC 
ACCTCGCTGG TCATCCCGCT CATCGGCGAG TACCTCCTCT TCACCATGAT CTTCGTCACC 
CTCTCCATCG TCATCACGGT CTTCGTGCTC AATGTGCACC ACCGCTCGCC ACGCACACAC 
ACGATGCCCG CCTGGGTGCG TAGAGTCTTC CTGGACATCG TGCCTCGCCT CCTCTTCATG 
AAGCGCCCCT CTGTGGTCAA AGACAACTGC CGGAGACTTA TTGAGTCCAT GCACAAGATG 
GCCAACGCCC CCCGCTTCTG GCCAGAGCCT GTGGGCGAGC CCGGCATCTT GAGTGACATC 
TGCAACCAAG GTCTGTCACC TGCCCCAACT TTCTGCAACC CCACGGACAC AGCAGTCGAG 
ACCCAGCCTA CGTGCAGGTC ACCCCCCCTT GAGGTCCCTG ACTTGAAGAC ATCAGAGGTT 
GAGAAGGCCA GTCCCTGTCC ATCGCCTGGC TCCTGTCCTC CACCCAAGAG CAGCAGTGGG 
GCTCCAATGC TCATCAAAGC CAGGTCCCTG AGTGTCCAGC ATGTGCCCAG CTCCCAAGAA 
GCAGCAGAAG ATGGCATCCG CTGCCGGTCT CGGAGTATCC AGTACTGTGT TTCCCAAGAT 
GGAGCTGCCT CCCTGGCTGA CAGCAAGCCC ACCAGCTCCC CGACCTCCCT GAAGGCCCGT 
CCATCCCAGC TTCCCGTGTC AGACCAGGCC TCTCCATGCA AATGCACATG CAAGGAACCA 
TCTCCTGTGT CCCCAGTCAC TGTGCTCAAG GCGGGAGGCA CCAAAGCACC TCCCCAACAC 
CTGCCCCTGT CACCAGCCCT GACACGGGCA GTAGAAGGCG TCCAGTACAT TGCAGACCAC 
CTCAAGGCAG AAGACACTGA CTTCTCGGTG AAGGAGGACT GGAAATACGT GGCCATGGTC 
ATTGACCGAA TCTTCCTCTG GATGTTCATC ATTGTCTGCC TTCTGGGCAC TGTGGGACTC 
TTCCTGCCTC CCTGGCTGGC TGGTATGATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acute Ethanol Administration Upregulates Synaptic ?4-Subunit of Neuronal Nicotinic Acetylcholine Receptors within the Nucleus Accumbens and Amygdala.
Frontiers in molecular neuroscience10338(2017)
Tarren JR,Lester HA,Belmer A,Bartlett SE

Molecular simulation study of the unbinding of ?-conotoxin [?4E]GID at the ?7 and ?4?2 neuronal nicotinic acetylcholine receptors.
Journal of molecular graphics & modelling70109-121(2016 Nov)
Suresh A,Hung A

Inhibited Expression of ?
Cellular and molecular neurobiology36(8)1377-1387(2016 Nov)
Xiao Y,Zhao L,Kuang SX,Guan ZZ

Mechanisms of inhibition and potentiation of ?4?2 nicotinic acetylcholine receptors by members of the Ly6 protein family.
The Journal of biological chemistry290(40)24509-18(2015 Oct)
Wu M,Puddifoot CA,Taylor P,Joiner WJ

The modulation of nicotinic acetylcholine receptors on the neuronal network oscillations in rat hippocampal CA3 area.
Scientific reports59493(2015 Mar)
Wang Y,Wang Z,Wang J,Wang Y,Henderson Z,Wang X,Zhang X,Song J,Lu C

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Chrna4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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