Alpl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alpl
Entrez Gene ID	25586
Full Name	alkaline phosphatase, liver/bone/kidney
Synonyms	Akp2,PHOA
General protein information	Preferred Names alkaline phosphatase, liver/bone/kidney Names alkaline phosphatase, tissue-nonspecific isozyme AP-TNAP TNSALP Tissue-nonspecific ALP alkaline phosphatase alkaline phosphatase 2, liver alkaline phosphatase liver/bone/kidney isozyme tissue-nonspecific alkaline phosphatase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	catalyzes the conversion of an orthophosphoric monoester to an alcohol phosphate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017593165.1	XP_017448654.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
XM_006239136.3	XP_006239198.1	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
NM_013059.1	NP_037191.1	alkaline phosphatase, tissue-nonspecific isozyme precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ALPL	NP_789828.2
Canis lupus familiaris (dog)	ALPL	NP_001184066.1
Rattus norvegicus (Norway rat)	Alpl	NP_037191.1
Gallus gallus (chicken)	ALPL	NP_990691.1
Drosophila melanogaster (fruit fly)	CG8147	NP_649897.4
Homo sapiens (human)	ALPL	NP_000469.3
	ALPL	XP_001109717.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001684	XP_321411.3
Pan troglodytes (chimpanzee)	ALPL	XP_003307915.1
Xenopus tropicalis (tropical clawed frog)	LOC100485467	XP_002943485.2
Mus musculus (house mouse)	Alpl	NP_001274101.1
Danio rerio (zebrafish)	alp3	NP_001265768.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of aluminum trichloride on the cartilage stimulatory growth factors in rats.
Zhang F, Sun X, Yu H, Yang X, Song M, Han Y, Li Y, Zhu Y
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine30(1)143-150(2017 Feb)

Nonylphenol induces liver toxicity and oxidative stress in rat.
Kazemi S, Mousavi Kani SN, Ghasemi-Kasman M, Aghapour F, Khorasani H, Moghadamnia AA
Biochemical and biophysical research communications479(1)17-21(2016 10)

Stratified organization and disorganization of inner plexiform layer revealed by TNAP activity in healthy and diabetic rat retina.
K?ntor O, Varga A, T?th R, ?nzs?ly A, P?lfi E, Kov?cs-?ller T, Nitschke R, Sz?l ?, Sz?kely A, V?lgyi B, N?gyessy L, Somogyv?ri Z, Luk?ts ?
Cell and tissue research359(2)409-421(2015 Feb)

CD73-TNAP crosstalk regulates the hypertrophic response and cardiomyocyte calcification due to ?1 adrenoceptor activation.
Gan XT, Taniai S, Zhao G, Huang CX, Velenosi TJ, Xue J, Urquhart BL, Karmazyn M
Molecular and cellular biochemistry394(1-2)237-46(2014 Sep)

Intestinal alkaline phosphatase is protective to the preterm rat pup intestine.
Heinzerling NP, Liedel JL, Welak SR, Fredrich K, Biesterveld BE, Pritchard KA Jr, Gourlay DM
Journal of pediatric surgery49(6)954-60; discussion 960(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

he rats body weight were decreased, the cartilage histological structure were disrupted, the cartilage and serum contents of Al and the serum level of C-telopeptide of type II collagen were all increased, the serum level of type II collagen (Col II) and alkaline phosphatase (ALP), and the mRNA expressions of TGF-beta1, BMP-2, ALP and Col II were all decreased in the AlCl3-treated groups compared with those in control g...
Title: Effects of aluminum trichloride on the cartilage stimulatory growth factors in rats.

Data show that nonylphenol (NP) increased serum alkaline phosphatase, lead to liver damage and can increase the expression of heme oxygenase-1 (HO1) and growth arrest and DNA-damage-inducible 45 beta protein (Gadd45b) genes and may modify the toxic effects on liver through induction of oxidative stress.
Title: Nonylphenol induces liver toxicity and oxidative stress in rat.

TNAP histochemistry offers a more straightforward, but also more sensitive, method for investigating retinal strata and their diabetes-induced degeneration.
Title: Stratified organization and disorganization of inner plexiform layer revealed by TNAP activity in healthy and diabetic rat retina.

TNAP contributes to the hypertrophic effect of phenylephrine, as well as its ability to produce cardiomyocyte calcification. These responses are minimized by CD73-dependent endogenously produced adenosine.
Title: CD73-TNAP crosstalk regulates the hypertrophic response and cardiomyocyte calcification due to ?1 adrenoceptor activation.

Rat pups fed IAP had decreased mRNA expression of the inflammatory cytokines TNFalpha, IL-6 and iNOS.
Title: Intestinal alkaline phosphatase is protective to the preterm rat pup intestine.

The following Alpl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alpl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa28734	XM_006239136.3 Latest version!	Rattus norvegicus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa28734	XM_017593165.1 Latest version!	Rattus norvegicus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa01814	NM_013059.1 Latest version!	Rattus norvegicus alkaline phosphatase, biomineralization associated (Alpl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alpl ( XM_017593165.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448654.1 Rattus norvegicus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28734
Clone ID Related Accession (Same CDS sequence)	XM_017593165.1 , XM_006239136.3
Accession Version	XM_017593165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTTGC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC ATTTGTGCCA 
GAGAAAGAGA AAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAACTCAA CACCAACGTG GCCAAGAACA TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT GACAGCTGCC CGCATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACACGGCT GGAGATGGAC AAGTTCCCCT TTGTGGCTCT CTCCAAGACG 
TACAACACCA ACGCTCAGGT CCCCGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC CGTGGGAGTG AGCGCGGCCA CTGAGCGCAC GCGATGCAAC 
ACCACTCAGG GGAACGAGGT CACGTCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATCG TGACCACCAC TCGGGTGAAC CACGCCACTC CCAGTGCAGC CTATGCGCAC 
TCGGCCGATC GGGACTGGTA CTCGGACAAT GAGATGCCGC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCCTATCA GCTAATGCAC AACATCAAGG ACATCGATGT GATCATGGGT 
GGCGGCCGGA AGTACATGTA CCCCAAGAAC AGAACTGATG TGGAATATGA ACTGGATGAG 
AAGGCCAGGG GCACCAGACT GGATGGCCTG GACCTCATCA GCATTTGGAA GAGCTTCAAA 
CCTAGACACA AGCACTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAGTTGAAT 
CGGAACAACC TGACTGACCC TTCCCTCTCG GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACAAAGAATC CCAAAGGCTT CTTCTTGCTA GTGGAAGGAG GCAGGATTGA CCACGGGCAC 
CATGAAGGCA AGGCCAAGCA GGCGCTGCAT GAGGCCGTGG AGATGGATGA GGCCATCGGA 
AAGGCGGGCA CCATGACTTC CCAGAAAGAC ACGTTGACTG TGGTTACTGC TGATCACTCC 
CACGTTTTCA CGTTTGGTGG CTACACCCCC AGGGGCAACT CCATTTTTGG TCTGGCTCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACAGCCATCC TGTATGGCAA CGGGCCTGGT 
TACAAGGTGG TGGACGGTGA ACGGGAGAAC GTCTCCATGG TGGATTATGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT CCCCCTGCGG CACGAGACCC ACGGTGGGGA AGATGTGGCG 
GTCTTTGCCA AGGGCCCTAT GGCTCACCTG CTTCACGGCG TCCATGAGCA GAACTACATC 
CCCCACGTCA TGGCGTATGC CTCCTGCATT GGAGCCAACC TTGACCACTG TGCCTGGGCC 
AGCTCTGCGA GCAGCCCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTCT GTTCCCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448654.1
CDS	285..1859
Translation	MILPFLVLAIGTCLTNSFVPEKEKDPSYWRQQAQETLKNALKLQKLNTNVAKNIIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDEAIGKAGTMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWASSASSPSPGALLLPLALFPLRTLF

Target ORF information:

RefSeq Version	XM_017593165.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593165.1

ORF Insert Sequence:

ATGATCTTGC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC ATTTGTGCCA 
GAGAAAGAGA AAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAACTCAA CACCAACGTG GCCAAGAACA TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT GACAGCTGCC CGCATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACACGGCT GGAGATGGAC AAGTTCCCCT TTGTGGCTCT CTCCAAGACG 
TACAACACCA ACGCTCAGGT CCCCGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC CGTGGGAGTG AGCGCGGCCA CTGAGCGCAC GCGATGCAAC 
ACCACTCAGG GGAACGAGGT CACGTCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATCG TGACCACCAC TCGGGTGAAC CACGCCACTC CCAGTGCAGC CTATGCGCAC 
TCGGCCGATC GGGACTGGTA CTCGGACAAT GAGATGCCGC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCCTATCA GCTAATGCAC AACATCAAGG ACATCGATGT GATCATGGGT 
GGCGGCCGGA AGTACATGTA CCCCAAGAAC AGAACTGATG TGGAATATGA ACTGGATGAG 
AAGGCCAGGG GCACCAGACT GGATGGCCTG GACCTCATCA GCATTTGGAA GAGCTTCAAA 
CCTAGACACA AGCACTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAGTTGAAT 
CGGAACAACC TGACTGACCC TTCCCTCTCG GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACAAAGAATC CCAAAGGCTT CTTCTTGCTA GTGGAAGGAG GCAGGATTGA CCACGGGCAC 
CATGAAGGCA AGGCCAAGCA GGCGCTGCAT GAGGCCGTGG AGATGGATGA GGCCATCGGA 
AAGGCGGGCA CCATGACTTC CCAGAAAGAC ACGTTGACTG TGGTTACTGC TGATCACTCC 
CACGTTTTCA CGTTTGGTGG CTACACCCCC AGGGGCAACT CCATTTTTGG TCTGGCTCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACAGCCATCC TGTATGGCAA CGGGCCTGGT 
TACAAGGTGG TGGACGGTGA ACGGGAGAAC GTCTCCATGG TGGATTATGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT CCCCCTGCGG CACGAGACCC ACGGTGGGGA AGATGTGGCG 
GTCTTTGCCA AGGGCCCTAT GGCTCACCTG CTTCACGGCG TCCATGAGCA GAACTACATC 
CCCCACGTCA TGGCGTATGC CTCCTGCATT GGAGCCAACC TTGACCACTG TGCCTGGGCC 
AGCTCTGCGA GCAGCCCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTCT GTTCCCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( XM_006239136.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239198.1 Rattus norvegicus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa28734
Clone ID Related Accession (Same CDS sequence)	XM_017593165.1 , XM_006239136.3
Accession Version	XM_006239136.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alkaline phosphatase, tissue-nonspecific isozyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTTGC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC ATTTGTGCCA 
GAGAAAGAGA AAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAACTCAA CACCAACGTG GCCAAGAACA TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT GACAGCTGCC CGCATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACACGGCT GGAGATGGAC AAGTTCCCCT TTGTGGCTCT CTCCAAGACG 
TACAACACCA ACGCTCAGGT CCCCGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC CGTGGGAGTG AGCGCGGCCA CTGAGCGCAC GCGATGCAAC 
ACCACTCAGG GGAACGAGGT CACGTCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATCG TGACCACCAC TCGGGTGAAC CACGCCACTC CCAGTGCAGC CTATGCGCAC 
TCGGCCGATC GGGACTGGTA CTCGGACAAT GAGATGCCGC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCCTATCA GCTAATGCAC AACATCAAGG ACATCGATGT GATCATGGGT 
GGCGGCCGGA AGTACATGTA CCCCAAGAAC AGAACTGATG TGGAATATGA ACTGGATGAG 
AAGGCCAGGG GCACCAGACT GGATGGCCTG GACCTCATCA GCATTTGGAA GAGCTTCAAA 
CCTAGACACA AGCACTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAGTTGAAT 
CGGAACAACC TGACTGACCC TTCCCTCTCG GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACAAAGAATC CCAAAGGCTT CTTCTTGCTA GTGGAAGGAG GCAGGATTGA CCACGGGCAC 
CATGAAGGCA AGGCCAAGCA GGCGCTGCAT GAGGCCGTGG AGATGGATGA GGCCATCGGA 
AAGGCGGGCA CCATGACTTC CCAGAAAGAC ACGTTGACTG TGGTTACTGC TGATCACTCC 
CACGTTTTCA CGTTTGGTGG CTACACCCCC AGGGGCAACT CCATTTTTGG TCTGGCTCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACAGCCATCC TGTATGGCAA CGGGCCTGGT 
TACAAGGTGG TGGACGGTGA ACGGGAGAAC GTCTCCATGG TGGATTATGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT CCCCCTGCGG CACGAGACCC ACGGTGGGGA AGATGTGGCG 
GTCTTTGCCA AGGGCCCTAT GGCTCACCTG CTTCACGGCG TCCATGAGCA GAACTACATC 
CCCCACGTCA TGGCGTATGC CTCCTGCATT GGAGCCAACC TTGACCACTG TGCCTGGGCC 
AGCTCTGCGA GCAGCCCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTCT GTTCCCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239198.1
CDS	384..1958
Translation	MILPFLVLAIGTCLTNSFVPEKEKDPSYWRQQAQETLKNALKLQKLNTNVAKNIIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDEAIGKAGTMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWASSASSPSPGALLLPLALFPLRTLF

Target ORF information:

RefSeq Version	XM_006239136.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkaline phosphatase, liver/bone/kidney (Alpl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239136.3

ORF Insert Sequence:

ATGATCTTGC CATTTTTAGT ACTGGCCATC GGCACCTGCC TTACCAACTC ATTTGTGCCA 
GAGAAAGAGA AAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAACTCAA CACCAACGTG GCCAAGAACA TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT GACAGCTGCC CGCATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACACGGCT GGAGATGGAC AAGTTCCCCT TTGTGGCTCT CTCCAAGACG 
TACAACACCA ACGCTCAGGT CCCCGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC CGTGGGAGTG AGCGCGGCCA CTGAGCGCAC GCGATGCAAC 
ACCACTCAGG GGAACGAGGT CACGTCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATCG TGACCACCAC TCGGGTGAAC CACGCCACTC CCAGTGCAGC CTATGCGCAC 
TCGGCCGATC GGGACTGGTA CTCGGACAAT GAGATGCCGC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCCTATCA GCTAATGCAC AACATCAAGG ACATCGATGT GATCATGGGT 
GGCGGCCGGA AGTACATGTA CCCCAAGAAC AGAACTGATG TGGAATATGA ACTGGATGAG 
AAGGCCAGGG GCACCAGACT GGATGGCCTG GACCTCATCA GCATTTGGAA GAGCTTCAAA 
CCTAGACACA AGCACTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAGTTGAAT 
CGGAACAACC TGACTGACCC TTCCCTCTCG GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACAAAGAATC CCAAAGGCTT CTTCTTGCTA GTGGAAGGAG GCAGGATTGA CCACGGGCAC 
CATGAAGGCA AGGCCAAGCA GGCGCTGCAT GAGGCCGTGG AGATGGATGA GGCCATCGGA 
AAGGCGGGCA CCATGACTTC CCAGAAAGAC ACGTTGACTG TGGTTACTGC TGATCACTCC 
CACGTTTTCA CGTTTGGTGG CTACACCCCC AGGGGCAACT CCATTTTTGG TCTGGCTCCC 
ATGGTGAGTG ACACGGACAA GAAGCCCTTC ACAGCCATCC TGTATGGCAA CGGGCCTGGT 
TACAAGGTGG TGGACGGTGA ACGGGAGAAC GTCTCCATGG TGGATTATGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT CCCCCTGCGG CACGAGACCC ACGGTGGGGA AGATGTGGCG 
GTCTTTGCCA AGGGCCCTAT GGCTCACCTG CTTCACGGCG TCCATGAGCA GAACTACATC 
CCCCACGTCA TGGCGTATGC CTCCTGCATT GGAGCCAACC TTGACCACTG TGCCTGGGCC 
AGCTCTGCGA GCAGCCCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTCT GTTCCCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpl ( NM_013059.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037191.1 Rattus norvegicus alkaline phosphatase, biomineralization associated (Alpl), mRNA.

Price & Availability↑

CloneID	ORa01814
Clone ID Related Accession (Same CDS sequence)	NM_013059.1
Accession Version	NM_013059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	alkaline phosphatase, tissue-nonspecific isozyme precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03572.1. On Nov 4, 2002 this sequence version replaced NM_134342.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03572.1, BC088399.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATCTTGC CATTTTTAGT ACTGGCCATC GGACCCTGCC TTACCAACTC ATTTGTGCCA 
GAGAAAGAGA AAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAACTCAA CACCAACGTG GCCAAGAACA TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT GACAGCTGCC CGCATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACACGGCT GGAGATGGAC AAGTTCCCCT TTGTGGCTCT CTCCAAGACG 
TACAACACCA ACGCTCAGGT CCCCGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC CGTGGGAGTG AGCGCGGCCA CTGAGCGCAC GCGATGCAAC 
ACCACTCAGG GGAACGAGGT CACGTCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATCG TGACCACCAC TCGGGTGAAC CACGCCACTC CCAGTGCAGC CTATGCGCAC 
TCGGCCGATC GGGACTGGTA CTCGGACAAT GAGATGCGCC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCCTATCA GCTAATGCAC AACATCAAGG ACATCGATGT GATCATGGGT 
GGTGGCCGGA AGTACATGTA CCCCAAGAAC AGAACTGATG TGGAATATGA ACTGGATGAG 
AAGGCCAGGG GCACCAGACT GGATGGCCTG GACCTCATCA GCATTTGGAA GAGCTTCAAA 
CCTAGACACA AGCACTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAGTTGAAT 
CGGAACAACC TGACTGACCC TTCCCTCTCG GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACAAAGAATC CCAAAGGCTT CTTCTTGCTA GTGGAAGGAG GCAGGATTGA CCACGGGCAC 
CATGAAGGCA AGGCCAAGCA GGCGCTGCAT GAGGCCGTGG AGATGGATGA GGCCATCGGA 
AAGGCGGGCA CCATGACTTC CCAGAAAGAC ACGTTGACTG TGGTTACTGC TGATCACTCC 
CACGTTTTCA CGTTTGGTGG CTACACCCCC AGGGGCAACT CCATTTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACAGCCATCC TGTATGGCAA CGGGCCTGGT 
TACAAGGTGG TGGACGGTGA ACGGGAGAAC GTCTCCATGG TGGATTATGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT CCCCCTGCGG CACGAGACCC ACGGTGGGGA AGATGTGGCG 
GTCTTTGCCA AGGGCCCTAT GGCTCACCTG CTTCACGGCG TCCATGAGCA GAACTACATC 
CCCCACGTCA TGGCGTATGC CTCCTGCATT GGAGCCAACC TTGACCACTG TGCCTGGGCC 
AGCTCTGCGA GCAGCCCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTCT GTTCCCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037191.1
CDS	153..1727
Translation	MILPFLVLAIGPCLTNSFVPEKEKDPSYWRQQAQETLKNALKLQKLNTNVAKNIIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMRPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDEAIGKAGTMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWASSASSPSPGALLLPLALFPLRTLF

Target ORF information:

RefSeq Version	NM_013059.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkaline phosphatase, biomineralization associated (Alpl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013059.1

ORF Insert Sequence:

ATGATCTTGC CATTTTTAGT ACTGGCCATC GGACCCTGCC TTACCAACTC ATTTGTGCCA 
GAGAAAGAGA AAGACCCCAG TTACTGGCGA CAGCAAGCCC AAGAGACCTT GAAAAATGCC 
CTGAAACTCC AAAAACTCAA CACCAACGTG GCCAAGAACA TCATCATGTT CCTGGGAGAT 
GGTATGGGCG TCTCCACAGT GACAGCTGCC CGCATCCTTA AGGGCCAGCT ACACCACAAC 
ACGGGCGAGG AGACACGGCT GGAGATGGAC AAGTTCCCCT TTGTGGCTCT CTCCAAGACG 
TACAACACCA ACGCTCAGGT CCCCGACAGC GCGGGCACTG CCACTGCCTA CTTGTGTGGC 
GTGAAGGCCA ACGAGGGCAC CGTGGGAGTG AGCGCGGCCA CTGAGCGCAC GCGATGCAAC 
ACCACTCAGG GGAACGAGGT CACGTCCATC CTGCGCTGGG CCAAGGATGC TGGGAAGTCC 
GTGGGCATCG TGACCACCAC TCGGGTGAAC CACGCCACTC CCAGTGCAGC CTATGCGCAC 
TCGGCCGATC GGGACTGGTA CTCGGACAAT GAGATGCGCC CAGAGGCTCT GAGCCAGGGC 
TGCAAGGACA TCGCCTATCA GCTAATGCAC AACATCAAGG ACATCGATGT GATCATGGGT 
GGTGGCCGGA AGTACATGTA CCCCAAGAAC AGAACTGATG TGGAATATGA ACTGGATGAG 
AAGGCCAGGG GCACCAGACT GGATGGCCTG GACCTCATCA GCATTTGGAA GAGCTTCAAA 
CCTAGACACA AGCACTCCCA CTATGTCTGG AACCGCACTG AACTGCTGGC CCTTGACCCC 
TCCAGGGTGG ACTACCTCTT AGGTCTCTTT GAGCCCGGGG ACATGCAGTA TGAGTTGAAT 
CGGAACAACC TGACTGACCC TTCCCTCTCG GAGATGGTGG AGGTGGCCCT CCGGATCCTG 
ACAAAGAATC CCAAAGGCTT CTTCTTGCTA GTGGAAGGAG GCAGGATTGA CCACGGGCAC 
CATGAAGGCA AGGCCAAGCA GGCGCTGCAT GAGGCCGTGG AGATGGATGA GGCCATCGGA 
AAGGCGGGCA CCATGACTTC CCAGAAAGAC ACGTTGACTG TGGTTACTGC TGATCACTCC 
CACGTTTTCA CGTTTGGTGG CTACACCCCC AGGGGCAACT CCATTTTTGG TCTGGCTCCC 
ATGGTGAGCG ACACGGACAA GAAGCCCTTC ACAGCCATCC TGTATGGCAA CGGGCCTGGT 
TACAAGGTGG TGGACGGTGA ACGGGAGAAC GTCTCCATGG TGGATTATGC TCACAACAAC 
TACCAGGCCC AGTCCGCTGT CCCCCTGCGG CACGAGACCC ACGGTGGGGA AGATGTGGCG 
GTCTTTGCCA AGGGCCCTAT GGCTCACCTG CTTCACGGCG TCCATGAGCA GAACTACATC 
CCCCACGTCA TGGCGTATGC CTCCTGCATT GGAGCCAACC TTGACCACTG TGCCTGGGCC 
AGCTCTGCGA GCAGCCCCTC CCCAGGGGCC CTGCTGCTTC CACTGGCTCT GTTCCCCCTA 
CGCACCCTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of aluminum trichloride on the cartilage stimulatory growth factors in rats.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine30(1)143-150(2017 Feb)
Zhang F,Sun X,Yu H,Yang X,Song M,Han Y,Li Y,Zhu Y

Nonylphenol induces liver toxicity and oxidative stress in rat.
Biochemical and biophysical research communications479(1)17-21(2016 10)
Kazemi S,Mousavi Kani SN,Ghasemi-Kasman M,Aghapour F,Khorasani H,Moghadamnia AA

Stratified organization and disorganization of inner plexiform layer revealed by TNAP activity in healthy and diabetic rat retina.
Cell and tissue research359(2)409-421(2015 Feb)
K?ntor O,Varga A,T?th R,?nzs?ly A,P?lfi E,Kov?cs-?ller T,Nitschke R,Sz?l ?,Sz?kely A,V?lgyi B,N?gyessy L,Somogyv?ri Z,Luk?ts ?

CD73-TNAP crosstalk regulates the hypertrophic response and cardiomyocyte calcification due to ?1 adrenoceptor activation.
Molecular and cellular biochemistry394(1-2)237-46(2014 Sep)
Gan XT,Taniai S,Zhao G,Huang CX,Velenosi TJ,Xue J,Urquhart BL,Karmazyn M

Intestinal alkaline phosphatase is protective to the preterm rat pup intestine.
Journal of pediatric surgery49(6)954-60; discussion 960(2014 Jun)
Heinzerling NP,Liedel JL,Welak SR,Fredrich K,Biesterveld BE,Pritchard KA Jr,Gourlay DM

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Alpl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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