Trh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trh
Entrez Gene ID	25569
Full Name	thyrotropin releasing hormone
Synonyms	Pro-TRH,THR,TRH01,Trf
General protein information	Preferred Names thyrotropin releasing hormone Names thyrotropin releasing hormone TSH-releasing factor pro-thyrotropin-releasing hormone prothyroliberin protirelin thyrotropin-releasing factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The rat proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin, and may be involved in cardiac function in heart failure. Disruption of this gene is associated with hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. [provided by RefSeq, May 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013046.3	NP_037178.1	thyrotropin releasing hormone preproprotein
XM_006236899.2	XP_006236961.1	thyrotropin releasing hormone isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRH	NP_009048.1
	TRH	XP_001093550.2
Mus musculus (house mouse)	Trh	NP_033452.2
Rattus norvegicus (Norway rat)	Trh	NP_037178.1
Pan troglodytes (chimpanzee)	TRH	XP_001144565.1
Bos taurus (cattle)	TRH	NP_001094691.1
Canis lupus familiaris (dog)	TRH	XP_005632182.1

Gene Ontology
Bibliography

Related articles in PubMed

Intrauterine Zn Deficiency Favors Thyrotropin-Releasing Hormone-Increasing Effects on Thyrotropin Serum Levels and Induces Subclinical Hypothyroidism in Weaned Rats.
Alc?ntara-Alonso V, Alvarez-Salas E, Matamoros-Trejo G, de Gortari P
Nutrients9(10)(2017 Oct)

Mct8 and trh co-expression throughout the hypothalamic paraventricular nucleus is modified by dehydration-induced anorexia in rats.
Alvarez-Salas E, Mengod G, Garc?a-Luna C, Soberanes-Ch?vez P, Matamoros-Trejo G, de Gortari P
Neuropeptides5633-40(2016 Apr)

Mediobasal hypothalamic and adenohypophyseal TRH-degrading enzyme (PPII) is down-regulated by zinc deficiency.
Alvarez-Salas E, Alc?ntara-Alonso V, Matamoros-Trejo G, Vargas MA, Morales-Mulia M, de Gortari P
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience46115-24(2015 Nov)

Fasting Enhances Pyroglutamyl Peptidase II Activity in Tanycytes of the Mediobasal Hypothalamus of Male Adult Rats.
Lazcano I, Cabral A, Uribe RM, Jaimes-Hoy L, Perello M, Joseph-Bravo P, S?nchez-Jaramillo E, Charli JL
Endocrinology156(7)2713-23(2015 Jul)

TGF?2 regulates hypothalamic Trh expression through the TGF? inducible early gene-1 (TIEG1) during fetal development.
Mart?nez-Armenta M, D?az de Le?n-Guerrero S, Catal?n A, Alvarez-Arellano L, Uribe RM, Subramaniam M, Charli JL, P?rez-Mart?nez L
Molecular and cellular endocrinology400129-39(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that dehydration induced anorexia rats showed increased number of thyrotropin-releasing hormone (TRH) cells in caudal paraventricular hypothalamic nucleus, as well as a decreased percentage of TRH-expressing neurons that co-expressed monocarboxylate transporter-8 mRNA signal.
Title: Mct8 and trh co-expression throughout the hypothalamic paraventricular nucleus is modified by dehydration-induced anorexia in rats.

The results of this study concluded that long-term changes in dietary Zn down-regulate PPII activity
Title: Mediobasal hypothalamic and adenohypophyseal TRH-degrading enzyme (PPII) is down-regulated by zinc deficiency.

Delayed increases in both the median eminence PPII as well as the thyroliberinase activities in fasted male rats may facilitate the maintenance of deep downregulation of the HPT axis function, despite a partial reactivation of TRH expression in the PVN.
Title: Fasting Enhances Pyroglutamyl Peptidase II Activity in Tanycytes of the Mediobasal Hypothalamus of Male Adult Rats.

TGFbeta2 regulates hypothalamic TRH expression through TIEG1 during fetal development.
Title: TGF?2 regulates hypothalamic Trh expression through the TGF? inducible early gene-1 (TIEG1) during fetal development.

a specific TRH increase in the left ventricle induced structural changes in the normal heart
Title: Thyrotropin-releasing hormone overexpression induces structural changes of the left ventricle in the normal rat heart.

The following Trh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12660	NM_013046.3 Latest version!	Rattus norvegicus thyrotropin releasing hormone (Trh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa12660	XM_006236899.2 Latest version!	Rattus norvegicus thyrotropin releasing hormone (Trh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trh ( NM_013046.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037178.1 Rattus norvegicus thyrotropin releasing hormone (Trh), mRNA.

Price & Availability↑

CloneID	ORa12660
Clone ID Related Accession (Same CDS sequence)	NM_013046.3 , XM_006236899.2
Accession Version	NM_013046.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Rattus norvegicus(Norway rat)
Product	thyrotropin releasing hormone preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FQ100942.1, FQ223091.1 and FQ221609.1. On May 2, 2013 this sequence version replaced NM_013046.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ100942.1, FQ222015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689595, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGGAC CTTGGTTGCT GCTGGCTCTG GCTTTGATCT TCACCCTAAC TGGTATCCCT 
GAATCCTGCG CCTTGCCGGA GGCAGCCCAG GAGGAAGGTG CAGTGACTCC TGACCTTCCT 
GGCCTGGAGA ATGTTCAGGT CCGGCCAGAA CGTCGATTCT TGTGGAAAGA CCTCCAGCGG 
GTGAGAGGGG ACCTCGGTGC TGCCTTAGAC TCCTGGATCA CAAAACGCCA GCATCCAGGC 
AAAAGGGAGG AGGAGGAAAA AGACATTGAA GCTGAAGAGA GGGGAGACTT GGGAGAAGGG 
GGAGCCTGGA GACTCCACAA ACGACAGCAC CCCGGCCGAC GTGCCAACCA GGACAAGTAT 
TCATGGGCAG ATGAGGAGGA CAGTGACTGG ATGCCACGGT CCTGGTTACC AGATTTCTTT 
CTGGATTCCT GGTTCTCAGA TGTCCCCCAA GTCAAGCGGC AGCACCCTGG CAGGCGATCC 
TTCCCCTGGA TGGAGTCTGA TGTCACCAAG AGGCAACATC CAGGCCGGAG GTTCATAGAT 
CCCGAGCTCC AAAGAAGCTG GGAAGAAAAA GAGGGAGAGG GTGTCTTAAT GCCTGAGAAA 
CGCCAGCATC CTGGCAAAAG GGCATTGGGT CATCCCTGTG GGCCCCAGGG GACTTGTGGT 
CAAACAGGCC TGCTCCAGCT TCTAGGTGAC CTGAGCAGGG GTCAGGAGAC CCTGGTGAAG 
CAAAGCCCAC AAGTGGAACC CTGGGACAAG GAACCTCTGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037178.1
CDS	136..903
Translation	MPGPWLLLALALIFTLTGIPESCALPEAAQEEGAVTPDLPGLENVQVRPERRFLWKDLQRVRGDLGAALDSWITKRQHPGKREEEEKDIEAEERGDLGEGGAWRLHKRQHPGRRANQDKYSWADEEDSDWMPRSWLPDFFLDSWFSDVPQVKRQHPGRRSFPWMESDVTKRQHPGRRFIDPELQRSWEEKEGEGVLMPEKRQHPGKRALGHPCGPQGTCGQTGLLQLLGDLSRGQETLVKQSPQVEPWDKEPLEE

Target ORF information:

RefSeq Version	NM_013046.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thyrotropin releasing hormone (Trh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013046.3

ORF Insert Sequence:

ATGCCGGGAC CTTGGTTGCT GCTGGCTCTG GCTTTGATCT TCACCCTAAC TGGTATCCCT 
GAATCCTGCG CCTTGCCGGA GGCAGCCCAG GAGGAAGGTG CAGTGACTCC TGACCTTCCT 
GGCCTGGAGA ATGTTCAGGT CCGGCCAGAA CGTCGATTCT TGTGGAAAGA CCTCCAGCGG 
GTGAGAGGGG ACCTCGGTGC TGCCTTAGAC TCCTGGATCA CAAAACGCCA GCATCCAGGC 
AAAAGGGAGG AGGAGGAAAA AGACATTGAA GCTGAAGAGA GGGGAGACTT GGGAGAAGGG 
GGAGCCTGGA GACTCCACAA ACGACAGCAC CCCGGCCGAC GTGCCAACCA GGACAAGTAT 
TCATGGGCAG ATGAGGAGGA CAGTGACTGG ATGCCACGGT CCTGGTTACC AGATTTCTTT 
CTGGATTCCT GGTTCTCAGA TGTCCCCCAA GTCAAGCGGC AGCACCCTGG CAGGCGATCC 
TTCCCCTGGA TGGAGTCTGA TGTCACCAAG AGGCAACATC CAGGCCGGAG GTTCATAGAT 
CCCGAGCTCC AAAGAAGCTG GGAAGAAAAA GAGGGAGAGG GTGTCTTAAT GCCTGAGAAA 
CGCCAGCATC CTGGCAAAAG GGCATTGGGT CATCCCTGTG GGCCCCAGGG GACTTGTGGT 
CAAACAGGCC TGCTCCAGCT TCTAGGTGAC CTGAGCAGGG GTCAGGAGAC CCTGGTGAAG 
CAAAGCCCAC AAGTGGAACC CTGGGACAAG GAACCTCTGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trh ( XM_006236899.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236961.1 Rattus norvegicus thyrotropin releasing hormone (Trh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12660
Clone ID Related Accession (Same CDS sequence)	NM_013046.3 , XM_006236899.2
Accession Version	XM_006236899.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	thyrotropin releasing hormone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006236899.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAC CTTGGTTGCT GCTGGCTCTG GCTTTGATCT TCACCCTAAC TGGTATCCCT 
GAATCCTGCG CCTTGCCGGA GGCAGCCCAG GAGGAAGGTG CAGTGACTCC TGACCTTCCT 
GGCCTGGAGA ATGTTCAGGT CCGGCCAGAA CGTCGATTCT TGTGGAAAGA CCTCCAGCGG 
GTGAGAGGGG ACCTCGGTGC TGCCTTAGAC TCCTGGATCA CAAAACGCCA GCATCCAGGC 
AAAAGGGAGG AGGAGGAAAA AGACATTGAA GCTGAAGAGA GGGGAGACTT GGGAGAAGGG 
GGAGCCTGGA GACTCCACAA ACGACAGCAC CCCGGCCGAC GTGCCAACCA GGACAAGTAT 
TCATGGGCAG ATGAGGAGGA CAGTGACTGG ATGCCACGGT CCTGGTTACC AGATTTCTTT 
CTGGATTCCT GGTTCTCAGA TGTCCCCCAA GTCAAGCGGC AGCACCCTGG CAGGCGATCC 
TTCCCCTGGA TGGAGTCTGA TGTCACCAAG AGGCAACATC CAGGCCGGAG GTTCATAGAT 
CCCGAGCTCC AAAGAAGCTG GGAAGAAAAA GAGGGAGAGG GTGTCTTAAT GCCTGAGAAA 
CGCCAGCATC CTGGCAAAAG GGCATTGGGT CATCCCTGTG GGCCCCAGGG GACTTGTGGT 
CAAACAGGCC TGCTCCAGCT TCTAGGTGAC CTGAGCAGGG GTCAGGAGAC CCTGGTGAAG 
CAAAGCCCAC AAGTGGAACC CTGGGACAAG GAACCTCTGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236961.1
CDS	567..1334
Translation	MPGPWLLLALALIFTLTGIPESCALPEAAQEEGAVTPDLPGLENVQVRPERRFLWKDLQRVRGDLGAALDSWITKRQHPGKREEEEKDIEAEERGDLGEGGAWRLHKRQHPGRRANQDKYSWADEEDSDWMPRSWLPDFFLDSWFSDVPQVKRQHPGRRSFPWMESDVTKRQHPGRRFIDPELQRSWEEKEGEGVLMPEKRQHPGKRALGHPCGPQGTCGQTGLLQLLGDLSRGQETLVKQSPQVEPWDKEPLEE

Target ORF information:

RefSeq Version	XM_006236899.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thyrotropin releasing hormone (Trh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236899.2

ORF Insert Sequence:

ATGCCGGGAC CTTGGTTGCT GCTGGCTCTG GCTTTGATCT TCACCCTAAC TGGTATCCCT 
GAATCCTGCG CCTTGCCGGA GGCAGCCCAG GAGGAAGGTG CAGTGACTCC TGACCTTCCT 
GGCCTGGAGA ATGTTCAGGT CCGGCCAGAA CGTCGATTCT TGTGGAAAGA CCTCCAGCGG 
GTGAGAGGGG ACCTCGGTGC TGCCTTAGAC TCCTGGATCA CAAAACGCCA GCATCCAGGC 
AAAAGGGAGG AGGAGGAAAA AGACATTGAA GCTGAAGAGA GGGGAGACTT GGGAGAAGGG 
GGAGCCTGGA GACTCCACAA ACGACAGCAC CCCGGCCGAC GTGCCAACCA GGACAAGTAT 
TCATGGGCAG ATGAGGAGGA CAGTGACTGG ATGCCACGGT CCTGGTTACC AGATTTCTTT 
CTGGATTCCT GGTTCTCAGA TGTCCCCCAA GTCAAGCGGC AGCACCCTGG CAGGCGATCC 
TTCCCCTGGA TGGAGTCTGA TGTCACCAAG AGGCAACATC CAGGCCGGAG GTTCATAGAT 
CCCGAGCTCC AAAGAAGCTG GGAAGAAAAA GAGGGAGAGG GTGTCTTAAT GCCTGAGAAA 
CGCCAGCATC CTGGCAAAAG GGCATTGGGT CATCCCTGTG GGCCCCAGGG GACTTGTGGT 
CAAACAGGCC TGCTCCAGCT TCTAGGTGAC CTGAGCAGGG GTCAGGAGAC CCTGGTGAAG 
CAAAGCCCAC AAGTGGAACC CTGGGACAAG GAACCTCTGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intrauterine Zn Deficiency Favors Thyrotropin-Releasing Hormone-Increasing Effects on Thyrotropin Serum Levels and Induces Subclinical Hypothyroidism in Weaned Rats.
Nutrients9(10)(2017 Oct)
Alc?ntara-Alonso V,Alvarez-Salas E,Matamoros-Trejo G,de Gortari P

Mct8 and trh co-expression throughout the hypothalamic paraventricular nucleus is modified by dehydration-induced anorexia in rats.
Neuropeptides5633-40(2016 Apr)
Alvarez-Salas E,Mengod G,Garc?a-Luna C,Soberanes-Ch?vez P,Matamoros-Trejo G,de Gortari P

Mediobasal hypothalamic and adenohypophyseal TRH-degrading enzyme (PPII) is down-regulated by zinc deficiency.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience46115-24(2015 Nov)
Alvarez-Salas E,Alc?ntara-Alonso V,Matamoros-Trejo G,Vargas MA,Morales-Mulia M,de Gortari P

Fasting Enhances Pyroglutamyl Peptidase II Activity in Tanycytes of the Mediobasal Hypothalamus of Male Adult Rats.
Endocrinology156(7)2713-23(2015 Jul)
Lazcano I,Cabral A,Uribe RM,Jaimes-Hoy L,Perello M,Joseph-Bravo P,S?nchez-Jaramillo E,Charli JL

TGF?2 regulates hypothalamic Trh expression through the TGF? inducible early gene-1 (TIEG1) during fetal development.
Molecular and cellular endocrinology400129-39(2015 Jan)
Mart?nez-Armenta M,D?az de Le?n-Guerrero S,Catal?n A,Alvarez-Arellano L,Uribe RM,Subramaniam M,Charli JL,P?rez-Mart?nez L

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Trh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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