Eloa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eloa
Entrez Gene ID	25562
Full Name	elongin A
Synonyms	El,Tceb3
General protein information	Preferred Names elongin A Names elongin-A RNA polymerase II transcription factor SIII subunit A RNA polymerase II transcription factor SIII subunit A1 RNA polymerase II transcription factor elongin subunit A (110 kDa) SIII p110 elongin 110 kDa subunit transcription elongation factor B (SIII), polypeptide 3 transcription elongation factor B polypeptide 3 transcription elongation factor B subunit 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	subunit A of the transcription factor B (SIII) complex (also known as elongin); frees RNA Polymerase II from transient pausing at template-encoded arresting sites [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017103.2	NP_058799.2	elongin-A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC100851883	XP_005203441.1
Pan troglodytes (chimpanzee)	TCEB3	XP_513196.3
	TCEB3	XP_001103322.2
Canis lupus familiaris (dog)	TCEB3	XP_005617985.1
Mus musculus (house mouse)	Tceb3	NP_038764.2
Xenopus tropicalis (tropical clawed frog)	tceb3	XP_002933890.2
Homo sapiens (human)	TCEB3	NP_003189.2
Rattus norvegicus (Norway rat)	Tceb3	NP_058799.2
Gallus gallus (chicken)	TCEB3	NP_001026076.1
Danio rerio (zebrafish)	zgc:55573	NP_998620.1

Gene Ontology
Bibliography

Related articles in PubMed

Cockayne syndrome B protein regulates recruitment of the Elongin A ubiquitin ligase to sites of DNA damage.
Weems JC, Slaughter BD, Unruh JR, Boeing S, Hall SM, McLaird MB, Yasukawa T, Aso T, Svejstrup JQ, Conaway JW, Conaway RC
The Journal of biological chemistry292(16)6431-6437(2017 04)

Transcriptional properties of mammalian elongin A and its role in stress response.
Kawauchi J, Inoue M, Fukuda M, Uchida Y, Yasukawa T, Conaway RC, Conaway JW, Aso T, Kitajima S
The Journal of biological chemistry288(34)24302-15(2013 Aug)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase.
Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW
The Journal of biological chemistry276(32)29748-53(2001 Aug)

The inducible elongin A elongation activation domain: structure, function and interaction with the elongin BC complex.
Aso T, Haque D, Barstead RJ, Conaway RC, Conaway JW
The EMBO journal15(20)5557-66(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Elongin A transcription elongation activity, but not its ubiquitination activity, is most important for its function in induction of transcription of ATF3 and p21.
Title: Transcriptional properties of mammalian elongin A and its role in stress response.

The following Eloa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eloa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02846	NM_017103.2 Latest version!	Rattus norvegicus elongin A (Eloa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eloa ( NM_017103.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058799.2 Rattus norvegicus elongin A (Eloa), mRNA.

Price & Availability↑

CloneID	ORa02846
Clone ID Related Accession (Same CDS sequence)	NM_017103.2
Accession Version	NM_017103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	elongin-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081815.1. On Jun 10, 2007 this sequence version replaced NM_017103.1. ##Evidence-Data-START## Transcript exon combination :: BC081815.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGG AGTCGGCGCT CCAAGTTGTG GAGAAGCTGC AGGCGCGCCT GGCTGCGAAC 
CCGGACCCCA AGAAGCTATT GAAATATTTG AAGAAACTTT CCGTCTTACC TATTACAGTA 
GACATTCTTG TGGAGACTGG GGTGGGGAAA ACAGTGAACA GCTTTCGGAA ACATGAGCAA 
GTGGGAAACT TTGCCAGAGA CCTGGTTGCC CAGTGGAAGA AGCTGGTTCC AGTAGAACGA 
AACAATGAGG CTGAGGATCA GGATTTTGAG AAGAGCAATT CCCGCAAGCG TCCCCGAGAT 
GTTCCCCAGC AGGAGGAGGA AGCGGAGGGG AACTACCAGG AAAGCTGGCA AGCCTCAGGC 
AGCCAGCCCT ACAGCCCTGA GCACAGACAG AAAAAGCACA GAAAACTTCC TGAGCTTGAA 
AGGCCTCACA AAGTGGCTCA TGGTCACGAG AGGAGAGATG AAAGGAAGAG GTGTCACAAA 
GTGTCACCAC CATATTCTTC AGACCCCGAG TCGTCTGACT ATGGTCATGT TCAATCTCCT 
CCACCTTCAA GTCCCCATCA AATGTATACA GACCTCTCTA GGTCCCCAGA GATGGACCAG 
GAACCCATCG TCTCACACCC GAAGCCTGGG AAAGTCCACA GTAATACCTT TCAGGACAGA 
CTAGGGGTTA GTCACCTGGG TGAGCACCAA GGGAAAGGGG CTGTTAGCCA AAACAAGCCA 
CACAAATCTT CCCACAAGGA GAAACGCCCT GTGGATGCCA GGGGGGATGA GAAGAGCTCT 
GTCATGGGCA GAGAGAAGTC ACACAAAGCC TCTTCCAAAG AGGAGAGCCG AAGGCTACTC 
TCAGAGGACA GTGCCAAGGA GAAGCTGCCC TCCAGTGTTG TCAAGAAAGA GAAGGACAGA 
GAAGGCAACA GCCTCAAGAA GAAGTTGTCA CCTGCCTTAG ATGTTGCTTC AGACAACCAC 
TTTAAAAAGC CCAAACACAA GGACTCCGAG AAAATCAAGT CTGACAAAAA CAAGCAGAGT 
GTAGATAGCG TGGACTCAGG ACGAGGGACA GGAGACCCAT TACCCAGAGC CAAGGATAAA 
GTTCCCAACA ACCTGAAGGC TCAGGAGGGG AAAGTAAGAA CTAACTCGGA TCGAAAGTCA 
CCAGGCTCAC TCCCTAAAGT AGAAGAGATG GACATGGATG ATGAGTTTGA GCAGCCCACC 
ATGTCCTTTG AGTCATACCT CAGCTATGAC CAGCCCCGCA AGAAAAAGAA GAAGGTTGTG 
AAAACTTCCG GTACAGCACT TGGAGAAAAA GGACTTAAAA AGAAGGACTC TAAAAGCACT 
AGTAAAAACT TGAACTCGGC TCAGAAATTA CCCAAGGTGA ATGAAAACAA GTCAGACAAG 
TTGCAGCCAG CTGGAGCCGA ACCCACGAGG CCTAGAAAGG TCCCTACTGA TGTGCTGCCA 
GCATTGCCAG ACATCCCCTT GCCCGCCATA CAAACCAACT ATCGTCCCCT TCCCTCCCTC 
GAGTTGATCT CCTCCTTTCA GCCAAAGCGA AAAGCTTTCT CTTCACCCCA GGAAGAAGAA 
GAAGCTGGGT TCACAGGACG CAGAATGAAT TCTAAGATGC AGGTGTATTC AGGTTCCAAG 
TGTGCCTATC TCCCCAAAAT GATGACCTTG CACCAGCAGT GTATCCGGGT GCTTAAGAAT 
AATATTGACT CCATCTTTGA AGTGGGAGGA GTCCCCTATT CTGTTCTTGA ACCTGTCTTG 
GAGAGGTGCA CACCCGATCA GCTCTACCGA ATAGAGGAAT GCAATCATGT ATTAATTGAG 
GAAACAGATC AGTTGTGGAA AGTTCACTGT CACCGGGACT TTAAGGAAGA AAGACCAGAA 
GAGTATGAGT CTTGGAGGGA GATGTACCTG AGGCTTCAGG ACGCCCGAGA GCAGCGGCTG 
CGCCTGCTCA CAAACAACAT CCGGTCTGCA CATGCCAATA AGCCAAAAGG ACGACAAGCA 
AAGATGGCCT TTGTGAACTC TGTGGCCAAG CCACCGAGAG ATGTTCGACG GAGGCAGGAG 
AAGTTTGGAA CCGGGGGAGC AGCTGTCCCT GAGAAAGTCA GGATTAAGCC AGCACCATAT 
ACAACAGGAA GCAGCCATGT TCCTGCCAGC AACAGCAGCA GCAGCTTCCA CTCAAGTCCT 
GAGGAGCTGG CCTACGAAGG GCCCAGTACC AGCAGTGCCC ACTTAGCTCC TGTGGCCAGC 
AGCTCTGTTT CCTATGATCC CAGGAAACCA GCTGTGAAGA AAATTGCCCC GATGATGGCC 
AAGACCATTA AAGCATTCAA GAACAGATTT TCCCGACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058799.2
CDS	67..2388
Translation	MAAESALQVVEKLQARLAANPDPKKLLKYLKKLSVLPITVDILVETGVGKTVNSFRKHEQVGNFARDLVAQWKKLVPVERNNEAEDQDFEKSNSRKRPRDVPQQEEEAEGNYQESWQASGSQPYSPEHRQKKHRKLPELERPHKVAHGHERRDERKRCHKVSPPYSSDPESSDYGHVQSPPPSSPHQMYTDLSRSPEMDQEPIVSHPKPGKVHSNTFQDRLGVSHLGEHQGKGAVSQNKPHKSSHKEKRPVDARGDEKSSVMGREKSHKASSKEESRRLLSEDSAKEKLPSSVVKKEKDREGNSLKKKLSPALDVASDNHFKKPKHKDSEKIKSDKNKQSVDSVDSGRGTGDPLPRAKDKVPNNLKAQEGKVRTNSDRKSPGSLPKVEEMDMDDEFEQPTMSFESYLSYDQPRKKKKKVVKTSGTALGEKGLKKKDSKSTSKNLNSAQKLPKVNENKSDKLQPAGAEPTRPRKVPTDVLPALPDIPLPAIQTNYRPLPSLELISSFQPKRKAFSSPQEEEEAGFTGRRMNSKMQVYSGSKCAYLPKMMTLHQQCIRVLKNNIDSIFEVGGVPYSVLEPVLERCTPDQLYRIEECNHVLIEETDQLWKVHCHRDFKEERPEEYESWREMYLRLQDAREQRLRLLTNNIRSAHANKPKGRQAKMAFVNSVAKPPRDVRRRQEKFGTGGAAVPEKVRIKPAPYTTGSSHVPASNSSSSFHSSPEELAYEGPSTSSAHLAPVASSSVSYDPRKPAVKKIAPMMAKTIKAFKNRFSRR

Target ORF information:

RefSeq Version	NM_017103.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus elongin A (Eloa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017103.2

ORF Insert Sequence:

ATGGCGGCGG AGTCGGCGCT CCAAGTTGTG GAGAAGCTGC AGGCGCGCCT GGCTGCGAAC 
CCGGACCCCA AGAAGCTATT GAAATATTTG AAGAAACTTT CCGTCTTACC TATTACAGTA 
GACATTCTTG TGGAGACTGG GGTGGGGAAA ACAGTGAACA GCTTTCGGAA ACATGAGCAA 
GTGGGAAACT TTGCCAGAGA CCTGGTTGCC CAGTGGAAGA AGCTGGTTCC AGTAGAACGA 
AACAATGAGG CTGAGGATCA GGATTTTGAG AAGAGCAATT CCCGCAAGCG TCCCCGAGAT 
GTTCCCCAGC AGGAGGAGGA AGCGGAGGGG AACTACCAGG AAAGCTGGCA AGCCTCAGGC 
AGCCAGCCCT ACAGCCCTGA GCACAGACAG AAAAAGCACA GAAAACTTCC TGAGCTTGAA 
AGGCCTCACA AAGTGGCTCA TGGTCACGAG AGGAGAGATG AAAGGAAGAG GTGTCACAAA 
GTGTCACCAC CATATTCTTC AGACCCCGAG TCGTCTGACT ATGGTCATGT TCAATCTCCT 
CCACCTTCAA GTCCCCATCA AATGTATACA GACCTCTCTA GGTCCCCAGA GATGGACCAG 
GAACCCATCG TCTCACACCC GAAGCCTGGG AAAGTCCACA GTAATACCTT TCAGGACAGA 
CTAGGGGTTA GTCACCTGGG TGAGCACCAA GGGAAAGGGG CTGTTAGCCA AAACAAGCCA 
CACAAATCTT CCCACAAGGA GAAACGCCCT GTGGATGCCA GGGGGGATGA GAAGAGCTCT 
GTCATGGGCA GAGAGAAGTC ACACAAAGCC TCTTCCAAAG AGGAGAGCCG AAGGCTACTC 
TCAGAGGACA GTGCCAAGGA GAAGCTGCCC TCCAGTGTTG TCAAGAAAGA GAAGGACAGA 
GAAGGCAACA GCCTCAAGAA GAAGTTGTCA CCTGCCTTAG ATGTTGCTTC AGACAACCAC 
TTTAAAAAGC CCAAACACAA GGACTCCGAG AAAATCAAGT CTGACAAAAA CAAGCAGAGT 
GTAGATAGCG TGGACTCAGG ACGAGGGACA GGAGACCCAT TACCCAGAGC CAAGGATAAA 
GTTCCCAACA ACCTGAAGGC TCAGGAGGGG AAAGTAAGAA CTAACTCGGA TCGAAAGTCA 
CCAGGCTCAC TCCCTAAAGT AGAAGAGATG GACATGGATG ATGAGTTTGA GCAGCCCACC 
ATGTCCTTTG AGTCATACCT CAGCTATGAC CAGCCCCGCA AGAAAAAGAA GAAGGTTGTG 
AAAACTTCCG GTACAGCACT TGGAGAAAAA GGACTTAAAA AGAAGGACTC TAAAAGCACT 
AGTAAAAACT TGAACTCGGC TCAGAAATTA CCCAAGGTGA ATGAAAACAA GTCAGACAAG 
TTGCAGCCAG CTGGAGCCGA ACCCACGAGG CCTAGAAAGG TCCCTACTGA TGTGCTGCCA 
GCATTGCCAG ACATCCCCTT GCCCGCCATA CAAACCAACT ATCGTCCCCT TCCCTCCCTC 
GAGTTGATCT CCTCCTTTCA GCCAAAGCGA AAAGCTTTCT CTTCACCCCA GGAAGAAGAA 
GAAGCTGGGT TCACAGGACG CAGAATGAAT TCTAAGATGC AGGTGTATTC AGGTTCCAAG 
TGTGCCTATC TCCCCAAAAT GATGACCTTG CACCAGCAGT GTATCCGGGT GCTTAAGAAT 
AATATTGACT CCATCTTTGA AGTGGGAGGA GTCCCCTATT CTGTTCTTGA ACCTGTCTTG 
GAGAGGTGCA CACCCGATCA GCTCTACCGA ATAGAGGAAT GCAATCATGT ATTAATTGAG 
GAAACAGATC AGTTGTGGAA AGTTCACTGT CACCGGGACT TTAAGGAAGA AAGACCAGAA 
GAGTATGAGT CTTGGAGGGA GATGTACCTG AGGCTTCAGG ACGCCCGAGA GCAGCGGCTG 
CGCCTGCTCA CAAACAACAT CCGGTCTGCA CATGCCAATA AGCCAAAAGG ACGACAAGCA 
AAGATGGCCT TTGTGAACTC TGTGGCCAAG CCACCGAGAG ATGTTCGACG GAGGCAGGAG 
AAGTTTGGAA CCGGGGGAGC AGCTGTCCCT GAGAAAGTCA GGATTAAGCC AGCACCATAT 
ACAACAGGAA GCAGCCATGT TCCTGCCAGC AACAGCAGCA GCAGCTTCCA CTCAAGTCCT 
GAGGAGCTGG CCTACGAAGG GCCCAGTACC AGCAGTGCCC ACTTAGCTCC TGTGGCCAGC 
AGCTCTGTTT CCTATGATCC CAGGAAACCA GCTGTGAAGA AAATTGCCCC GATGATGGCC 
AAGACCATTA AAGCATTCAA GAACAGATTT TCCCGACGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cockayne syndrome B protein regulates recruitment of the Elongin A ubiquitin ligase to sites of DNA damage.
The Journal of biological chemistry292(16)6431-6437(2017 04)
Weems JC,Slaughter BD,Unruh JR,Boeing S,Hall SM,McLaird MB,Yasukawa T,Aso T,Svejstrup JQ,Conaway JW,Conaway RC

Transcriptional properties of mammalian elongin A and its role in stress response.
The Journal of biological chemistry288(34)24302-15(2013 Aug)
Kawauchi J,Inoue M,Fukuda M,Uchida Y,Yasukawa T,Conaway RC,Conaway JW,Aso T,Kitajima S

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase.
The Journal of biological chemistry276(32)29748-53(2001 Aug)
Kamura T,Burian D,Yan Q,Schmidt SL,Lane WS,Querido E,Branton PE,Shilatifard A,Conaway RC,Conaway JW

The inducible elongin A elongation activation domain: structure, function and interaction with the elongin BC complex.
The EMBO journal15(20)5557-66(1996 Oct)
Aso T,Haque D,Barstead RJ,Conaway RC,Conaway JW

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Eloa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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