Sirpa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sirpa
Entrez Gene ID	25528
Full Name	signal-regulatory protein alpha
Synonyms	Bit,Ptpns1,SHPS-1
General protein information	Preferred Names signal-regulatory protein alpha Names tyrosine-protein phosphatase non-receptor type substrate 1 Brain immunoglobulin like protein with tyrosine - based activation motifs CD172 antigen-like family member A Protein tyrosine phosphatase, non-receptor type substrate 1 (SHP substrate 1) SHP substrate 1 brain Ig-like molecule with tyrosine-based activation motifs inhibitory receptor SHPS-1 macrophage fusion receptor macrophage membrane protein MFP150 signal-regulatory protein alpha-1 sirp-alpha-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36
Summary	plays a role in macrophage fusion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591497.1	XP_017446986.1	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X2
NM_013016.2	NP_037148.2	tyrosine-protein phosphatase non-receptor type substrate 1 precursor
XM_006234951.3	XP_006235013.1	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
XM_006234953.3	XP_006235015.1	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
XM_017591496.1	XP_017446985.1	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
XM_017591498.1	XP_017446987.1	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
XM_006234952.3	XP_006235014.1	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIRPA	XP_514466.4
	SIRPA	XP_001109719.2
Rattus norvegicus (Norway rat)	Sirpa	NP_037148.2
Gallus gallus (chicken)	LOC771734	XP_001234976.3
Mus musculus (house mouse)	Sirpa	NP_001171117.1
Gallus gallus (chicken)	SIRPA	XP_004950046.1
Homo sapiens (human)	SIRPA	NP_001035111.1
Bos taurus (cattle)	SIRPA	NP_786982.1
Canis lupus familiaris (dog)	SIRPA	XP_005634938.1

Gene Ontology
Bibliography

Related articles in PubMed

Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7.
Nagamachi A, Matsui H, Asou H, Ozaki Y, Aki D, Kanai A, Takubo K, Suda T, Nakamura T, Wolff L, Honda H, Inaba T
Cancer cell24(3)305-17(2013 Sep)

MicroRNA-17/20a/106a modulate macrophage inflammatory responses through targeting signal-regulatory protein ?.
Zhu D, Pan C, Li L, Bian Z, Lv Z, Shi L, Zhang J, Li D, Gu H, Zhang CY, Liu Y, Zen K
The Journal of allergy and clinical immunology132(2)426-36.e8(2013 Aug)

Inflammation-induced proteolytic processing of the SIRP? cytoplasmic ITIM in neutrophils propagates a proinflammatory state.
Zen K, Guo Y, Bian Z, Lv Z, Zhu D, Ohnishi H, Matozaki T, Liu Y
Nature communications42436(2013)

SIRP? interacts with nephrin at the podocyte slit diaphragm.
Kajiho Y, Harita Y, Kurihara H, Horita S, Matsunaga A, Tsurumi H, Kanda S, Sugawara N, Miura K, Sekine T, Hattori S, Hattori M, Igarashi T
The FEBS journal279(17)3010-21(2012 Sep)

Disruption of the association of integrin-associated protein (IAP) with tyrosine phosphatase non-receptor type substrate-1 (SHPS)-1 inhibits pathophysiological changes in retinal endothelial function in a rat model of diabetes.
Maile LA, Gollahon K, Wai C, Byfield G, Hartnett ME, Clemmons D
Diabetologia55(3)835-44(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the glomeruli of CNS patients carrying mutations in NPHS1, where SD formation is disrupted, the expression of SIRPalpha as well as Neph1 and nephrin was significantly decreased, indicating that SIRPalpha is closely associated with the nephrin complex
Title: SIRP? interacts with nephrin at the podocyte slit diaphragm.

Data suggest that hyperglycemia induces increase in association of SHPS-1 with IAP (integrin-associated protein); this appears to be a generalized response of endothelial cells that contributes to endothelial cell dysfunction.
Title: Disruption of the association of integrin-associated protein (IAP) with tyrosine phosphatase non-receptor type substrate-1 (SHPS)-1 inhibits pathophysiological changes in retinal endothelial function in a rat model of diabetes.

Data demonstrate that BIT/SHPS-1 promotes the antiapoptotic effect of BDNF on the low potassium-induced cell death of cerebellar granule neuronss.
Title: BIT/SHPS-1 promotes antiapoptotic effect of BDNF on low potassium-induced cell death of cultured cerebellar granule neurons.

Data show that myelin down-regulates its own phagocytosis through CD47-SIRPalpha interactions.
Title: Myelin down-regulates myelin phagocytosis by microglia and macrophages through interactions between CD47 on myelin and SIRP? (signal regulatory protein-?) on phagocytes.

CD47-SIRP-alpha interaction may be functionally important in cell-cell communication in the diseased glomerulus.
Title: SIRP-alpha-CD47 system functions as an intercellular signal in the renal glomerulus.

The following Sirpa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sirpa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa26345	XM_017591498.1 Latest version!	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa26345	XM_006234951.3 Latest version!	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa26345	XM_006234952.3 Latest version!	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa26345	XM_017591496.1 Latest version!	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa26345	XM_006234953.3 Latest version!	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa01657	NM_013016.2 Latest version!	Rattus norvegicus signal-regulatory protein alpha (Sirpa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa60276	XM_017591497.1 Latest version!	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sirpa ( XM_006234951.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235013.1 Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa26345
Clone ID Related Accession (Same CDS sequence)	XM_006234951.3 , XM_006234953.3 , XM_017591496.1 , XM_017591498.1 , XM_006234952.3
Accession Version	XM_006234951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235013.1
CDS	432..1973
Translation	MEPAGPAPGRLGPLLFCLLLSASCFCAGASGKELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAREITQVQSLIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNRAQPTPKPEPSFSEYASVQVQRK

Target ORF information:

RefSeq Version	XM_006234951.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234951.3

ORF Insert Sequence:

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirpa ( XM_006234953.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235015.1 Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26345
Clone ID Related Accession (Same CDS sequence)	XM_006234951.3 , XM_006234953.3 , XM_017591496.1 , XM_017591498.1 , XM_006234952.3
Accession Version	XM_006234953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235015.1
CDS	550..2091
Translation	MEPAGPAPGRLGPLLFCLLLSASCFCAGASGKELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAREITQVQSLIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNRAQPTPKPEPSFSEYASVQVQRK

Target ORF information:

RefSeq Version	XM_006234953.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234953.3

ORF Insert Sequence:

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirpa ( XM_017591496.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446985.1 Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa26345
Clone ID Related Accession (Same CDS sequence)	XM_006234951.3 , XM_006234953.3 , XM_017591496.1 , XM_017591498.1 , XM_006234952.3
Accession Version	XM_017591496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446985.1
CDS	479..2020
Translation	MEPAGPAPGRLGPLLFCLLLSASCFCAGASGKELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAREITQVQSLIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNRAQPTPKPEPSFSEYASVQVQRK

Target ORF information:

RefSeq Version	XM_017591496.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591496.1

ORF Insert Sequence:

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirpa ( XM_017591498.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446987.1 Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X6, mRNA.

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CloneID	ORa26345
Clone ID Related Accession (Same CDS sequence)	XM_006234951.3 , XM_006234953.3 , XM_017591496.1 , XM_017591498.1 , XM_006234952.3
Accession Version	XM_017591498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446987.1
CDS	162..1703
Translation	MEPAGPAPGRLGPLLFCLLLSASCFCAGASGKELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAREITQVQSLIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNRAQPTPKPEPSFSEYASVQVQRK

Target ORF information:

RefSeq Version	XM_017591498.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591498.1

ORF Insert Sequence:

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirpa ( XM_006234952.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235014.1 Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X4, mRNA.

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CloneID	ORa26345
Clone ID Related Accession (Same CDS sequence)	XM_006234951.3 , XM_006234953.3 , XM_017591496.1 , XM_017591498.1 , XM_006234952.3
Accession Version	XM_006234952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235014.1
CDS	368..1909
Translation	MEPAGPAPGRLGPLLFCLLLSASCFCAGASGKELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAREITQVQSLIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNRAQPTPKPEPSFSEYASVQVQRK

Target ORF information:

RefSeq Version	XM_006234952.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234952.3

ORF Insert Sequence:

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGGTACAGTC TTTGATCCAG GACACAAATG ACATCAACGA CATCACATAC 
GCAGACCTGA ATCTGCCCAA AGAGAAGAAG CCCGCCCCCC GGGTCCCCGA GCCCAACAAC 
CACACAGAAT ATGCAAGCAT TGAGACAGGC AAACTGCCTA GGCCAGAGGA TACCCTCACC 
TATGCTGACC TGGACATGGT CCACCTCAAC CGGGCACAGC CAACCCCCAA GCCTGAGCCA 
TCCTTCTCAG AGTATGCCAG TGTCCAAGTC CAGAGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirpa ( NM_013016.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037148.2 Rattus norvegicus signal-regulatory protein alpha (Sirpa), mRNA.

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CloneID	ORa01657
Clone ID Related Accession (Same CDS sequence)	NM_013016.2
Accession Version	NM_013016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type substrate 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U62328.1. On Jun 9, 2003 this sequence version replaced NM_013016.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U62328.1, D38468.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTTCTGCTC 
TCCGCCTCCT GTATCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAAATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGATCCAGGA CACAAATGAC ATCAACGACA TCACATACGC AGACCTGAAT 
CTGCCCAAAG AGAAGAAGCC CGCCCCCCGG GTCCCCGAGC CCAACAACCA CACAGAATAT 
GCAAGCATTG AGACAGGCAA ACTGCCTAGG CCAGAGGATA CCCTCACCTA TGCTGACCTG 
GACATGGTCC ACCTCAACCG GGCACAGCCA ACCCCCAAGC CTGAGCCATC CTTCTCAGAG 
TATGCCAGTG TCCAAGTCCA GAGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037148.2
CDS	234..1763
Translation	MEPAGPAPGRLGPLLFCLLLSASCICAGASGKELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKNGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAREITQIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNRAQPTPKPEPSFSEYASVQVQRK

Target ORF information:

RefSeq Version	NM_013016.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus signal-regulatory protein alpha (Sirpa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013016.2

ORF Insert Sequence:

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTTCTGCTC 
TCCGCCTCCT GTATCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAAATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGATCCAGGA CACAAATGAC ATCAACGACA TCACATACGC AGACCTGAAT 
CTGCCCAAAG AGAAGAAGCC CGCCCCCCGG GTCCCCGAGC CCAACAACCA CACAGAATAT 
GCAAGCATTG AGACAGGCAA ACTGCCTAGG CCAGAGGATA CCCTCACCTA TGCTGACCTG 
GACATGGTCC ACCTCAACCG GGCACAGCCA ACCCCCAAGC CTGAGCCATC CTTCTCAGAG 
TATGCCAGTG TCCAAGTCCA GAGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sirpa ( XM_017591497.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446986.1 Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X5, mRNA.

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CloneID	ORa60276
Clone ID Related Accession (Same CDS sequence)	XM_017591497.1
Accession Version	XM_017591497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tyrosine-protein phosphatase non-receptor type substrate 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGATCCAGGA CACAAATGAC ATCAACGACA TCACATACGC AGACCTGAAT 
CTGCCCAAAG AGAAGAAGCC CGCCCCCCGG GTCCCCGAGC CCAACAACCA CACAGAATAT 
GCAAGCATTG AGACAGGCAA ACTGCCTAGG CCAGAGGATA CCCTCACCTA TGCTGACCTG 
GACATGGTCC ACCTCAACCG GGCACAGCCA ACCCCCAAGC CTGAGCCATC CTTCTCAGAG 
TATGCCAGTG TCCAAGTCCA GAGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446986.1
CDS	549..2078
Translation	MEPAGPAPGRLGPLLFCLLLSASCFCAGASGKELKVTQADKSVSVAAGDSATLNCTVSSLTPVGPIKWFKGEGQNRSPIYSFIGGEHFPRITNVSDATKRNNMDFSICISNVTPEDAGTYYCVKFQKGIVEPDTEIKSGGGTTLYVLAKPSSPEVSGPDSRGSPGQTVNFTCKSYGFSPRNITLKWLKDGKELSHLETTISSKSNVSYNISSTVSVKLSPEDIHSRVICEVAHVTLEGRPLNGTANFSNIIRVSPTLKITQQPLTPASQVNLTCQVQKFYPKALQLNWLENGNLSRTDKPEHFTDNRDGTYNYTSLFLVNSSAHREDVVFTCQVEHDSQPAITENHTVRAFAHSSSGGSMETIPDNNAYYNWNVFIGVGVACALLVVLLMAALYLLRIKQKKAKGSTSSTRLHEPEKNAREITQIQDTNDINDITYADLNLPKEKKPAPRVPEPNNHTEYASIETGKLPRPEDTLTYADLDMVHLNRAQPTPKPEPSFSEYASVQVQRK

Target ORF information:

RefSeq Version	XM_017591497.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus signal-regulatory protein alpha (Sirpa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591497.1

ORF Insert Sequence:

ATGGAGCCCG CCGGCCCGGC CCCTGGCCGC CTAGGGCCGC TGCTGTTCTG CCTGCTGCTC 
TCCGCGTCCT GTTTCTGTGC AGGAGCCAGC GGGAAAGAAC TGAAGGTGAC TCAGGCTGAC 
AAATCAGTGT CTGTTGCTGC TGGAGATTCG GCCACTCTGA ACTGCACTGT GTCCTCCCTG 
ACGCCTGTGG GACCCATTAA GTGGTTCAAA GGAGAAGGGC AAAATCGGAG CCCGATCTAC 
AGTTTCATAG GAGGAGAACA CTTTCCTCGA ATTACAAATG TTTCAGATGC TACTAAGAGA 
AACAATATGG ACTTTAGCAT CTGTATCAGT AATGTCACCC CAGAAGATGC TGGCACCTAC 
TACTGTGTGA AGTTCCAGAA AGGAATAGTA GAGCCTGACA CAGAAATTAA ATCTGGAGGG 
GGAACAACGC TCTATGTACT CGCCAAACCT TCTTCACCGG AAGTATCGGG CCCAGACTCC 
AGGGGCTCTC CTGGACAGAC AGTGAACTTC ACCTGCAAGT CTTACGGCTT CTCTCCCCGG 
AATATCACCC TGAAGTGGCT CAAAGATGGG AAAGAACTCT CCCATTTGGA GACCACCATC 
TCCAGTAAAA GCAATGTCTC CTACAACATC TCCAGCACAG TCAGCGTGAA ACTAAGCCCC 
GAGGACATTC ATTCTCGGGT CATCTGCGAG GTAGCCCACG TCACCTTGGA AGGACGCCCG 
CTTAATGGGA CCGCTAACTT TTCTAACATC ATCCGAGTTT CACCCACCTT GAAGATCACC 
CAACAGCCCC TGACGCCCGC GAGCCAGGTG AACCTCACCT GCCAGGTGCA GAAGTTCTAC 
CCCAAGGCTC TCCAGCTGAA CTGGCTGGAG AATGGAAACT TATCACGGAC GGACAAGCCC 
GAGCATTTCA CAGACAACAG GGATGGGACC TATAATTACA CAAGCCTGTT CCTGGTGAAC 
TCATCTGCTC ACAGAGAGGA TGTGGTATTC ACGTGCCAGG TGGAGCATGA CAGTCAGCCA 
GCGATCACCG AAAACCATAC CGTGCGGGCA TTTGCCCACT CGAGTAGTGG AGGCAGCATG 
GAAACCATCC CTGATAATAA TGCTTACTAC AACTGGAACG TCTTCATCGG TGTGGGTGTG 
GCGTGTGCTT TGCTAGTAGT CCTGCTGATG GCTGCCCTCT ACCTCCTCCG AATCAAACAG 
AAGAAAGCCA AGGGCTCAAC TTCTTCCACA CGGTTGCACG AGCCCGAGAA GAATGCCAGG 
GAAATAACCC AGATCCAGGA CACAAATGAC ATCAACGACA TCACATACGC AGACCTGAAT 
CTGCCCAAAG AGAAGAAGCC CGCCCCCCGG GTCCCCGAGC CCAACAACCA CACAGAATAT 
GCAAGCATTG AGACAGGCAA ACTGCCTAGG CCAGAGGATA CCCTCACCTA TGCTGACCTG 
GACATGGTCC ACCTCAACCG GGCACAGCCA ACCCCCAAGC CTGAGCCATC CTTCTCAGAG 
TATGCCAGTG TCCAAGTCCA GAGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Haploinsufficiency of SAMD9L, an endosome fusion facilitator, causes myeloid malignancies in mice mimicking human diseases with monosomy 7.
Cancer cell24(3)305-17(2013 Sep)
Nagamachi A,Matsui H,Asou H,Ozaki Y,Aki D,Kanai A,Takubo K,Suda T,Nakamura T,Wolff L,Honda H,Inaba T

MicroRNA-17/20a/106a modulate macrophage inflammatory responses through targeting signal-regulatory protein ?.
The Journal of allergy and clinical immunology132(2)426-36.e8(2013 Aug)
Zhu D,Pan C,Li L,Bian Z,Lv Z,Shi L,Zhang J,Li D,Gu H,Zhang CY,Liu Y,Zen K

Inflammation-induced proteolytic processing of the SIRP? cytoplasmic ITIM in neutrophils propagates a proinflammatory state.
Nature communications42436(2013)
Zen K,Guo Y,Bian Z,Lv Z,Zhu D,Ohnishi H,Matozaki T,Liu Y

SIRP? interacts with nephrin at the podocyte slit diaphragm.
The FEBS journal279(17)3010-21(2012 Sep)
Kajiho Y,Harita Y,Kurihara H,Horita S,Matsunaga A,Tsurumi H,Kanda S,Sugawara N,Miura K,Sekine T,Hattori S,Hattori M,Igarashi T

Disruption of the association of integrin-associated protein (IAP) with tyrosine phosphatase non-receptor type substrate-1 (SHPS)-1 inhibits pathophysiological changes in retinal endothelial function in a rat model of diabetes.
Diabetologia55(3)835-44(2012 Mar)
Maile LA,Gollahon K,Wai C,Byfield G,Hartnett ME,Clemmons D

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Sirpa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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