Pcp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcp4
Entrez Gene ID	25510
Full Name	Purkinje cell protein 4
Synonyms	PEPZ19,pep-19
General protein information	Preferred Names Purkinje cell protein 4 Names calmodulin regulator protein PCP4 Neuron specific protein PEP-19 (Purkinje cell protein 4) brain-specific antigen PCP-4 brain-specific polypeptide PEP-19
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q11
Summary	The protein encoded by this gene regulates H1˚ and H3.3 histone synthesis by binding to H1˚ and H3.3 histone mRNAs. The encoded protein can bind calmodulin as well, providung a possible link between calcium-dependent signals and histone metabolism. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013002.4	NP_037134.1	calmodulin regulator protein PCP4 isoform PEP19
NM_001270538.2	NP_001257467.1	calmodulin regulator protein PCP4 isoform LPI

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PCP4	XP_853359.1
Bos taurus (cattle)	PCP4	NP_001071604.1
Mus musculus (house mouse)	Pcp4	NP_032817.1
Pan troglodytes (chimpanzee)	PCP4	XP_531603.2
Rattus norvegicus (Norway rat)	Pcp4	NP_037134.1
Gallus gallus (chicken)	PCP4	NP_001265077.1
Homo sapiens (human)	PCP4	NP_006189.2

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of PEP-19 suppresses angiotensin II-induced cardiomyocyte hypertrophy.
Xie YY, Sun MM, Lou XF, Zhang C, Han F, Zhang BY, Wang P, Lu YM
Journal of pharmacological sciences125(3)274-82(2014)

The calmodulin regulator protein, PEP-19, sensitizes ATP-induced Ca2+ release.
Wang X, Xiong LW, El Ayadi A, Boehning D, Putkey JA
The Journal of biological chemistry288(3)2040-8(2013 Jan)

Peptide 19-containing neurons in the medullary dorsal horn, subnuclei interpolaris and oralis, and nucleus principalis of the rat.
Suzuki T, Sato T, Sasaki R, Ichikawa H
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft194(4)321-8(2012 Jul)

Pcp4l1 contains an auto-inhibitory element that prevents its IQ motif from binding to calmodulin.
Morgan MA, Morgan JI
Journal of neurochemistry121(6)843-51(2012 Jun)

RNA-binding activity of the rat calmodulin-binding PEP-19 protein and of the long PEP-19 isoform.
Saladino P, Di Liegro CM, Proia P, Sala A, Schiera G, Lo Cicero A, Di Liegro I
International journal of molecular medicine29(2)141-5(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of PEP-19 suppresses angiotensin II-induced cardiomyocyte hypertrophy.
Title: Overexpression of PEP-19 suppresses angiotensin II-induced cardiomyocyte hypertrophy.

These findings suggest that PEP 19-ir neurons in the MDH may have a function in modulation of nociceptive and thermo-receptive signaling.
Title: Peptide 19-containing neurons in the medullary dorsal horn, subnuclei interpolaris and oralis, and nucleus principalis of the rat.

PEP-19 and LPI bind H1 histone mRNA and that this binding is affected by calmodulin.
Title: RNA-binding activity of the rat calmodulin-binding PEP-19 protein and of the long PEP-19 isoform.

PCP4 promotes neuroendocrine cell differentiation and neurotransmitter release by activating calmodulin function.
Title: Purkinje cell protein 4 positively regulates neurite outgrowth and neurotransmitter release.

PEP-19 degradation by calpain might produce an aberrant activation of calmodulin functions, which in turn causes neuronal cell death
Title: Degradation of PEP-19, a calmodulin-binding protein, by calpain is implicated in neuronal cell death induced by intracellular Ca2+ overload.

The following Pcp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01615	NM_013002.4 Latest version!	Rattus norvegicus Purkinje cell protein 4 (Pcp4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa42835	NM_001270538.2 Latest version!	Rattus norvegicus Purkinje cell protein 4 (Pcp4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcp4 ( NM_013002.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037134.1 Rattus norvegicus Purkinje cell protein 4 (Pcp4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa01615
Clone ID Related Accession (Same CDS sequence)	NM_013002.4
Accession Version	NM_013002.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	calmodulin regulator protein PCP4 isoform PEP19
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC142238.6 and CB613293.1. On Dec 9, 2014 this sequence version replaced NM_013002.3. Transcript Variant: This variant (1) represents the predominant transcript and encodes the smaller isoform (PEP19). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB613293.1, BC059147.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGAGA GACAAAGTGC TGGAGCGACC AATGGAAAAG ACAAGACATC AGGAGATAAT 
GATGGGCAGA AGAAGGTCCA AGAAGAATTT GATATCGACA TGGATGCACC AGAGACAGAG 
CGTGCAGCTG TGGCCATTCA GTCTCAGTTC AGAAAATTCC AGAAGAAAAA GGCAGGATCA 
CAGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037134.1
CDS	67..255
Translation	MSERQSAGATNGKDKTSGDNDGQKKVQEEFDIDMDAPETERAAVAIQSQFRKFQKKKAGSQS

Target ORF information:

RefSeq Version	NM_013002.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Purkinje cell protein 4 (Pcp4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013002.4

ORF Insert Sequence:

ATGAGTGAGA GACAAAGTGC TGGAGCGACC AATGGAAAAG ACAAGACATC AGGAGATAAT 
GATGGGCAGA AGAAGGTCCA AGAAGAATTT GATATCGACA TGGATGCACC AGAGACAGAG 
CGTGCAGCTG TGGCCATTCA GTCTCAGTTC AGAAAATTCC AGAAGAAAAA GGCAGGATCA 
CAGTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pcp4 ( NM_001270538.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257467.1 Rattus norvegicus Purkinje cell protein 4 (Pcp4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa42835
Clone ID Related Accession (Same CDS sequence)	NM_001270538.2
Accession Version	NM_001270538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Rattus norvegicus(Norway rat)
Product	calmodulin regulator protein PCP4 isoform LPI
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC142238.6, CB613293.1 and AABR07033707.1. On Dec 9, 2014 this sequence version replaced NM_001270538.1. Transcript Variant: This variant (2) contains an alternate exon compared to variant 1, which causes a frameshift and use of a different start codon compared to variant 1. The resulting isoform (LPI) has a longer and distinct N-terminus compared to isoform PEP19. Experimental support for this transcript and isoform is provided in PMID:22020791 and PMID:17273800. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ493658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAAGA CAAGACATCA GGAGATAATG GCAAAGTGCT CACTGCCAGA GGAGGAATGG 
AGAAGTGCCT GGCACGATCC AGCCAGAACC TGCTCGGTCT CTAAGAAAGC CAAGAGGATC 
TGCAGTGGAA ATGCCTCGCT GGAGGCTCCT GAAATATATG GGCAGAAGAA GGTCCAAGAA 
GAATTTGATA TCGACATGGA TGCACCAGAG ACAGAGCGTG CAGCTGTGGC CATTCAGTCT 
CAGTTCAGAA AATTCCAGAA GAAAAAGGCA GGATCACAGT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257467.1
CDS	98..382
Translation	MEKTRHQEIMAKCSLPEEEWRSAWHDPARTCSVSKKAKRICSGNASLEAPEIYGQKKVQEEFDIDMDAPETERAAVAIQSQFRKFQKKKAGSQS

Target ORF information:

RefSeq Version	NM_001270538.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Purkinje cell protein 4 (Pcp4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270538.2

ORF Insert Sequence:

ATGGAAAAGA CAAGACATCA GGAGATAATG GCAAAGTGCT CACTGCCAGA GGAGGAATGG 
AGAAGTGCCT GGCACGATCC AGCCAGAACC TGCTCGGTCT CTAAGAAAGC CAAGAGGATC 
TGCAGTGGAA ATGCCTCGCT GGAGGCTCCT GAAATATATG GGCAGAAGAA GGTCCAAGAA 
GAATTTGATA TCGACATGGA TGCACCAGAG ACAGAGCGTG CAGCTGTGGC CATTCAGTCT 
CAGTTCAGAA AATTCCAGAA GAAAAAGGCA GGATCACAGT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of PEP-19 suppresses angiotensin II-induced cardiomyocyte hypertrophy.
Journal of pharmacological sciences125(3)274-82(2014)
Xie YY,Sun MM,Lou XF,Zhang C,Han F,Zhang BY,Wang P,Lu YM

The calmodulin regulator protein, PEP-19, sensitizes ATP-induced Ca2+ release.
The Journal of biological chemistry288(3)2040-8(2013 Jan)
Wang X,Xiong LW,El Ayadi A,Boehning D,Putkey JA

Peptide 19-containing neurons in the medullary dorsal horn, subnuclei interpolaris and oralis, and nucleus principalis of the rat.
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft194(4)321-8(2012 Jul)
Suzuki T,Sato T,Sasaki R,Ichikawa H

Pcp4l1 contains an auto-inhibitory element that prevents its IQ motif from binding to calmodulin.
Journal of neurochemistry121(6)843-51(2012 Jun)
Morgan MA,Morgan JI

RNA-binding activity of the rat calmodulin-binding PEP-19 protein and of the long PEP-19 isoform.
International journal of molecular medicine29(2)141-5(2012 Feb)
Saladino P,Di Liegro CM,Proia P,Sala A,Schiera G,Lo Cicero A,Di Liegro I

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Pcp4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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